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| 1 | Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis显示文摘We report that a solo single-guide RNA(sg RNA) seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPRàassociated 9(CRISRP/Cas_9) to simultaneously edit multiple genes At RPL_(10)A, At RPL_(10)B and At RPL_(10)C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas_9 technology to create At RPL_(10) triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sg RNA seed has the advantage of reducing off-target gene-editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less-homologous sequences for multiple gene-editing in plants and other organisms. | Zhiming Yu Qiyuan Chen Weiwei Chen Xian Zhang Fengling Mei Pengcheng Zhang Mei Zhao Xiaohong Wang Nongnong Shi Stephen Jackson Yiguo Hong | 2018 | Journal of Integrative Plant Biology2018，60，5： | 10 |
| 2 | Synthesis of hexaaluminate catalysts for methane combustion by reverse microemulsion medium显示文摘The aim of this study is to synthesize the catalysts of Fe-and Mn-substituted hexaaluminate by reverse microemulsion medium for methane catalytic combustion application.Pseudo-ternary phase diagrams in quaternary microemulsion systems of cetyltrimethylammonium bromide(CTAB),n-butanol,n-octane,and water [or Al(NO3)3 solution] were presented.The effects of the alcohol chain length,ratio of sur-factant to cosurfactant,and salt concentration on the formation and stability of microemulsion systems were studied.The phase behavior of microemulsion systems was confirmed through the varying of the conductivity with the water content.The performance and structure of the catalysts,La(Mnx/Fex)Al12-x-O19-δ synthesized with the optimal parameter in the phase diagrams of microemulsions systems were characterized by BET,TG-DTA,and XRD.The micro fix-bed reactor was used to measure the catalytic activities of catalysts to methane combustion.The results showed that this synthesis method could yield non-agglomerated and highly dispersed precursors that would undergo crystallization at the lower temperature of 950℃.When temperature was raised up to 1050℃,the complete crystalline La-hexaaluminate was shaped.The hexaaluminate substituted with Fe had high-catalytic activity and stability at high temperature,while the Mn-substituted had higher catalytic activity at lower temperature.When the cooperation of Fe and Mn occurred,i.e.,LaFeMnAl10O19-δ exhibited a high surface area and catalytic activity to CH4 combustion,the CH4 light-off temperature was only 475℃ and the complete combustion temperature was 660℃.This was attributed to the synergistic effect between Fe and Mn. | BAI ShouLi WANG LiangShi SHI BingJie YANG PengCheng CUI MeiSheng LONG ZhiQi LI DianQing CHEN AiFan | 2009 | Science China Chemistry2009，52，1： | 6 |
| 3 | Au-Ag alloy nanoparticles with tunable cavity for plasmon-enhanced photocatalytic H2 evolution显示文摘Au-Ag alloy nanoparticles with different cavity sizes have great potential for improving photocatalytic performance due to their tunable plasmon effect.In this study,galvanic replacement was combined with co-reduction with the reaction kinetics processes regulated to rapidly synthesize Au-Ag hollow alloy nanoparticles with tunable cavity sizes.The position of the localized surface plasmon resonance(LSPR)peak could be effectively adjusted between 490 nm and 713 nm by decreasing the cavity size of the Au-Ag hollow nanoparticles from 35 nm to 20 nm.The plasmon-enhanced photocatalytic H2 evolution of alloy nanoparticles with different cavity sizes was investigated.Compared with pure P25(TiO2),intact and thin-shelled Au-Ag hollow nanoparticles(HNPs)-supported photocatalyst exhibited an increase in the photocatalytic H2 evolution rate from 0.48μmol h^−1 to 4μmol h^−1 under full-spectrum irradiation.This improved photocatalytic performance was likely due to the plasmon-induced electromagnetic field effect,which caused strong photogenerated charge separation,rather than the generation of hot electrons. | Xuanyu Yue Juan Hou Haifeng Zhao Pengcheng Wu Yali Guo Qin Shi Long Chen Shanglong Peng Zhiyong Liu Guozhong Cao | 2020 | Journal of Energy Chemistry2020，29，10： | 4 |
| 4 | Activation of various brain areas during acupuncture at Taixi (KI 3) and Qiuxu (GB 40) acupoints显示文摘A number of previous studies of acupuncture acupoint specificity have used sham acupoints,sham acupuncture or meridian acupoints at a great distance from each other as controls in functional MRI (fMRI) experiments.However,few studies have compared different meridian acupoints within the same segment,which are associated with similarly intense needle sensations.We performed fMRI on 12 healthy young volunteers and observed differences in brain activation elicited by acupuncture of the Taixi (KI 3) and Qiuxu (GB 40) acupoints.Acupuncture was applied at the Taixi and Qiuxu acupoints,using a multiple-block fMRI design with three blocks,involving three alternations of resting and task phases.After scanning,needle sensation was assessed.The behavioral results revealed that the subjective needle sensation was similar between the Taixi and Qiuxu acupoints.The fMRI results revealed that acupuncture at the right Taixi acupoint activated the right superior temporal gyrus (BA 22),left middle frontal gyrus (BA 46) and inferior frontal gyrus (BA 45),bilateral parietal lobe postcentral gyrus (BA 2),right parietal lobe (BA 3),and left parietal lobe (BA 40).Acupuncture at the right Qiuxu acupoint activated the left superior temporal gyrus (BA 42),right parietal lobe postcentral gyrus (BA 40,BA 43),right inferior frontal gyrus (BA 47),bilateral superior temporal gyrus (BA 22),and right insula BA13.These results suggest that the right Taixi and Qiuxu acupoints activated different brain areas. | Shangjie Chen Huajun Cheng Shanshan Wu Jun Chen Pengcheng Ran Xuemin Shi | 2011 | Neural Regeneration Research2011，6，12： | 4 |
| 5 | Integration of homogeneous and heterogeneous nucleation growth via 3D alloy framework for stable Na/K metal anode显示文摘Sodium/Potassium(Na/K)metal anodes have been considered as the promising anodes for next-generation Na/K secondary batteries owing to their ultrahigh specific capacity,low redox potential and low cost.However,their practical application is still hampered due to unstable solid electrolyte interphase,infinite volume change,and dendrite growth.Herein,we design a 3D-Na_(3)Bi/3D-K_(3)Bi alloy host which enables the homogeneous and heterogeneous nucleation growth of Na/K metal.The unique structure with periodic alternating of electron and ion conductivity improves the mass transfer kinetics and prevents the volume expansion during cycling.Meanwhile,the sodiophilicity of Na_(3)Bi/potassiophilicity of K_(3)Bi framework can avoid dendritic growth.Cycling lifespans over 700 h with 1 mAh cm^(−2)for 3D-Na_(3)Bi@Na electrode and about 450 h with 1 mAh cm^(−2)for 3D-K_(3)Bi@K electrode are achieved,respectively.3D-Na_(3)Bi@Na||Na_(3)V_(2)(PO_(4))3 full battery shows sustainable cycle performance over 400 cycles.This design provides a simple but effective approach for achieving safety of sodium/potassium metal anodes. | Shufen Ye Lifeng Wang Fanfan Liu Pengcheng Shi Yan Yu | 2021 | eScience2021，1，1： | 3 |
| 6 | Stretchable,Rehealable,Recyclable,and Reconfigurable Integrated Strain Sensor for Joint Motion and Respiration Monitoring显示文摘Cutting-edge technologies of stretchable,skin-mountable,and wearable electronics have attracted tremendous attention recently due to their very wide applications and promising performances.One direction of particular interest is to investigate novel properties in stretchable electronics by exploring multifunctional materials.Here,we report an integrated strain sensing system that is highly stretchable,rehealable,fully recyclable,and reconfigurable.This system consists of dynamic covalent thermoset polyimine as the moldable substrate and encapsulation,eutectic liquid metal alloy as the strain sensing unit and interconnects,and off-the-shelf chip components for measuring and magnifying functions.The device can be attached on different parts of the human body for accurately monitoring joint motion and respiration.Such a strain sensing system provides a reliable,economical,and ecofriendly solution to wearable technologies,with wide applications in health care,prosthetics,robotics,and biomedical devices. | Chuanqian Shi Zhanan Zou Zepeng Lei Pengcheng Zhu Guohua Nie Wei Zhang Jianliang Xiao | 2021 | Research2021，，1： | 3 |
| 7 | Comparative WGBS identifies genes that influence non-ripe phenotype in tomato epimutant Colourless non-ripening显示文摘Whole-genome bisulfite sequencing(WGBS)allows single-base resolution and genome-wide profiling of DNA methylation in plants and animals.This technology provides a powerful tool to identify genes that are potentially controlled by dynamic changes of DNA methylation and demethylation.However,naturally occurring epimutants are rare and genes under epigenetic regulation as well as their biological relevances are often difficult to define.In tomato,fruit development and ripening are a complex process that involves epigenetic control.We have taken the advantage of the tomato epimutant Colourless non-ripening(Cnr)and performed comparative mining of the WGBS datasets for the Cnr and Sl CMT3-silenced Cnr fruits.We compared DNA methylation profiles for the promoter sequences of approximately 5,000 bp immediately upstream of the coding region of a list of20 genes.Differentially methylated regions were found for some of these genes.Virus-induced gene silencing(VIGS)of differentially methylated gene Sl DET1 or Sl PDS resulted in unusual brown pigmentation in Cnr fruits.These results suggest that comparative WGBS coupled with VIGS can be used to identify genes that may contribute to the colourless unripe phenotype of fruit in the Cnr epimutant. | Weiwei Chen Zhiming Yu Junhua Kong Hui Wang Yichen Li Mei Zhao Xiaohong Wang Qianqian Zheng Nongnong Shi Pengcheng Zhang Silin Zhong Paul Hunter Mahmut Tor Yiguo Hong | 2018 | Science China(Life Sciences)2018，61，2： | 3 |
| 8 | Microstructural evolution and hardness of as-cast Be-Al-Sc-Zr alloy processed by laser surface remelting显示文摘As-cast beryllium-aluminum(Be-Al)alloy exhibits a coarse microstructure with pore defects due to a large solidification interval,greatly limiting its mechanical properties.In this research,the relationship between laser surface remelting process and microstructure and hardness of as-cast Be-Al-Sc-Zr alloy was established.The experimental results demonstrated that a pore-free refined microstructure of remelted layer was obtained by controlling the parameter of effective laser energy input.The microstructure of as-cast Be-Al-Sc-Zr alloy consisted of equiaxed grains with Al phase forming a continuous frame wrapping Be phase,which was significantly refined in the remelted zone(from 25μm to 2μm).The Vickers hardness in the remelted zone(approximately 210 HV)was approximately 3 times that of as-cast Be-Al-Sc-Zr alloy.Analysis of the Vickers hardness and the Be phase size showed a good agreement with a Hall-Petch equation.In addition,transmission electron microscopy(TEM),auger electron spectroscopy(AES)and X-ray diffraction(XRD)analysis evidenced that Sc and Zr elements formed a single blocky phase Be13(Scx,Zr1-x),which was also greatly refined from 8μm to 1.5μm in the remelted zone.The results obtained in this study indicate that the laser surface remelting allowed refining the microstructure and further strengthening the Vickers hardness of Be-Al-Sc-Zr alloy. | Qingdong XU Yu LUO Xiangdong LIU Lei YANG Shixiong HE Xin WANG Wenyuan WANG Tao SHI Ruiwen LI Pengcheng ZHANG | 2021 | Chinese Journal of Aeronautics2021，34，8： | 2 |
| 9 | Osteosarcoma metastasis: prospective role of ezrin显示文摘 | Yuanjin Zhang Ling Zhang Guofu Zhang Songbai Li Jun Duan Jie Cheng Guozhen Ding Chibing Zhou Jie Zhang Pengcheng Luo Dongbing Cai Lianghong Kuang Yichun Zhou Liqun Tong Xiangdong Yu Lifang Zhang Lijun Xu Li Yu Xiaomei Shi Aihong Ke | 2014 | Tumor Biology2014，，6： | 1 |
| 10 | Emerging phagocytosis checkpoints in cancer immunotherapy显示文摘Cancer immunotherapy,mainly including immune checkpoints-targeted therapy and the adoptive transfer of engineered immune cells,has revolutionized the oncology landscape as it utilizes patients’own immune systems in combating the cancer cells.Cancer cells escape immune surveillance by hijacking the corresponding inhibitory pathways via overexpressing checkpoint genes.Phagocytosis checkpoints,such as CD47,CD24,MHC-I,PD-L1,STC-1 and GD2,have emerged as essential checkpoints for cancer immunotherapy by functioning as“don’t eat me”signals or interacting with“eat me”signals to suppress immune responses.Phagocytosis checkpoints link innate immunity and adaptive immunity in cancer immunotherapy.Genetic ablation of these phagocytosis checkpoints,as well as blockade of their signaling pathways,robustly augments phagocytosis and reduces tumor size.Among all phagocytosis checkpoints,CD47 is the most thoroughly studied and has emerged as a rising star among targets for cancer treatment.CD47-targeting antibodies and inhibitors have been investigated in various preclinical and clinical trials.However,anemia and thrombocytopenia appear to be formidable challenges since CD47 is ubiquitously expressed on erythrocytes.Here,we review the reported phagocytosis checkpoints by discussing their mechanisms and functions in cancer immunotherapy,highlight clinical progress in targeting these checkpoints and discuss challenges and potential solutions to smooth the way for combination immunotherapeutic strategies that involve both innate and adaptive immune responses. | Yu’e Liu Yanjin Wang Yanrong Yang Linjun Weng Qi Wu Jin Zhang Pengcheng Zhao Lan Fang Yufeng Shi Ping Wang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 1 |
| 11 | Circ_ASPH promotes cholangiocarcinoma growth and metastasis through the miR-581/ATP-binding cassette transporter G1 signaling pathway显示文摘Dear Editor,Cholangiocarcinoma(CCA)is an aggressive malignant tumor of the hepatobiliary system,mainly originating from the cholangiocytes of small intrahepatic bile ducts[1].The overall survival of patients with CCA is still unfavorable[1].Circular RNAs(circRNAs)are a type of non-coding RNAs with limited protein-coding capacity[2]and have shown to exert regulatory effects in multiple diseases by interacting with certain miRNAs to inhibit their expression and functions[3].Emerging evidence indicates that circRNAs can directly bind to several RNA-binding proteins to execute their biological functions.Hence,determining the mechanisms underlying the progression of CCA and searching for new drugs from the perspective of circRNAs are imperative. | Yi Xu Pengcheng Kang Kaiming Leng Yue Yao Guanqun Liao Yi Han Guangjun Shi Xiangyu Zhong Yunfu Cui | 2020 | Cancer Communications2020，40，10： | 1 |
| 12 | Enhancing Anisotropy Barriers of Dysprosium(III) Single‐Ion Magnets显示文摘 | Gong‐JunChen Yun‐NanGuo Jin‐LeiTian JinkuiTang WenGu XinLiu Shi‐PingYan PengCheng Dai‐ZhengLiao | 2012 | Chem. Eur. J2012，，9： | 1 |
| 13 | Manipulating selenium molecular configuration in N/O dual-doped porous carbon for high performance potassium-ion storage显示文摘Potassium-selenium(K-Se)batteries have attracted more and more attention because of their high theoretical specific capacity and natural abundance of K resources.However,dissolution of polyselenides,large volume expansion during cycling and low utilization of Se remain great challenges,leading to poor rate capability and cycle life.Herein,N/O dual-doped carbon nanofibers with interconnected micro/mesopores(MMCFs)are designed as hosts to manipulate Se molecular configuration for advanced flexible K-Se batteries.The micropores play a role in confining small Se molecule(Se_(2–3)),which could inhibit the formation of polyselenides and work as physical barrier to stabilize the cycle performance.While the mesopores can confine long-chain Se(Se_(4–7)),promising sufficient Se loading and contributing to higher discharge voltage of the whole Se@MMCFs composite.The N/O co-doping and the 3D interpenetrating nanostructure improve electrical conductivity and keep the structure integrity after cycling.The obtained Se_(2–3)/Se_(4–7)@MMCFs electrode exhibits an unprecedented cycle life(395 mA h g^(−1) at 1 A g^(−1) after 2000 cycles)and high specific energy density(400 Wh kg^(−1),nearly twice the specific energy density of the Se_(2–3)@MMCFs).This study offers a rational design for the realization of a high energy density and long cycle life chalcogen cathode for energy storage. | Dongjun Li Lifeng Wang Xiaolong Cheng Yu Yao Yu Jiang Pengcheng Shi Ying Wu Xiaojun Wu Cheng Ma Yan Yua | 2021 | Journal of Energy Chemistry2021，30，11： | 1 |
| 14 | Meshfree Representation and Computation:Applications to Cardiac Motion Analysis显示文摘 | Huafeng Liu Pengcheng Shi | 2003 | Inf Process Med Imaging2003，18，7： | 1 |
| 15 | Meshfree Representation and Computation: Applications to Cardiac Motion Analysis显示文摘 | Huafeng Liu Pengcheng Shi | 2003 | Inf Process Med Imaging2003，18，7： | 1 |
| 16 | SEM studies on Leaves leaf cells of some mosses显示文摘 | Wu Pengcheng Shi Dingji Wang Meizhi | 1990 | Cathaya1990，2，： | 1 |
| 17 | SEM studies on Leaves and leaf cells of some mosses显示文摘 | Wu Pengcheng Shi Dingjii Wang Meizhi | 1990 | Cathaya1990，2，： | 1 |
| 18 | Microwave-treated graphite felt as the positive electrode for all-vanadium redox flow battery显示文摘 | Xiaoxin Wu Hongfeng Xu Pengcheng Xu Yang Shen Lu Lu Jicheng Shi Jie Fu Hong Zhao | 2014 | Journal of Power Sources2014，，： | 1 |
| 19 | Nonlocal Prior Bayesian Tomographic Reconstruction显示文摘 | Yang Chen Jianhua Ma Qianjin Feng Limin Luo Pengcheng Shi Wufan Chen | 2008 | Journal of Mathematical Imaging and Vision2008，，2： | 1 |
| 20 | Volumetric deformation analysis using mechanics-based data fusion : Applications in cardiac motion recovery 显示文摘 | Shi Pengcheng Sinusas AJ Constable RT | 1999 | International Journal of Computer Vision1999，35，1： | 1 |
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