|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Parkin deficiency accentuates chronic alcohol intake-induced tissue injury and autophagy defects in brain,liver and skeletal muscle显示文摘Alcoholism leads to organ injury including mitochondrial defect and apoptosis with evidence favoring a role for autophagy dysregulation in alcoholic damage.Parkin represents an autosomal recessive in herited gene for Parkins on's disease and an important member of selective autophagy for mitoch on dria.The associati on between Parkins on's disease and alcoholic injury remains elusive.This study aimed to examine the effect of parki n deficiency on chr onic alcohol in takeinduced organ injury in brain,liver and skeletal muscle(rectus femoris muscle).Adult parkinknockout(PRK-/-)and wild-type mice were placed on Liber-De Carli alcohol liquid diet(4%)for 12 weeks prior to assessment of liver enzymes,intraperitoneal glucose toleranee,protein carbonyl content apoptosis,hematoxylin and eosin morphological staining,and mitochondrial respiration(cytochrome c oxidase,NADH:cytochrome c reductase and succinate:cytochrome c reductase).Autophagy protein markers were monitored by western blot analysis.Our data revealed that chronic alcohol intake imposed liver injury as evidenced by elevated aspartate aminotransferase and alanine transaminase,glucose intoleranee,elevated protein carbonyl formation,apoptosis,focal inflammation,necrosis,microvesiculation,autophagy/mitophagy failure and dampened mitochondrial respiration(complexⅣ,complexesⅠandⅢ,and complexesⅡandⅢ)in the brain,liver and rectus femoris skeletal muscle.Although parkin ablation itself did not generate any notable effects on liver enzymes,insulin sensitivity,tissue carbonyl damage,apoptosis,tissue morphology,autophagy or mitochondrial respiration,it accentuated alcohol intake-induced tissue damage,apoptosis,morphological change,autophagy/mitophagy failure and mitochondrial injury without affecting insulin sensitivity.These data suggest that parkin plays an integral role in the preservation against alcohol-induced organ injury,apoptosis and mitochondrial damage. | Hu Peng Xing Qin Sainan Chen Asli E Ceylan Maolong Dong Zhaofen Lin Jun Ren | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 3 |
| 2 | Toxic Effects of Three Heavy Metallic Ions on Rana zhenhaiensis Tadpoles显示文摘Heavy metal pollution is widespread in some areas of China and results in contamination of land, water, and air with which all living organisms interact. In this study, we used three heavy metallic ions(Cu2+, Pb2+ and Zn2+) to assess their toxicity effects on mortality, blood biomarker and growth traits(body length and body mass) of Rana zhenhaiensis tadpoles. The results showed that the toxicity levels of the three metallic ions were different when conducted with different experiment designs. For acute toxicity tests, Cu2+ was the most toxic with the highest tadpole mortality. The mortalities of tadpoles showed significant differences among the treatments at the same exposure time endpoints(24, 48, 72 and 96h). Results from repeated measures ANOVA indicated that metallic ion concentration, exposure time and their interactions significantly affected the mortalities of R. zhenhaiensis tadpoles. Also, the toxicity effects of all binary combinations of the three metallic ion treatments showed synergism. The half lethal concentrations(LC50) decreased with increasing exposure time during the experimental period, and the safe concentration(SC) values of Cu2+, Pb2+ and Zn2+ were different from each other. Combined and compared LC50 values with previous data reported, it is suggestes that the toxicity levels of metal pollution to anuran tadpoles should be species-and age-related. For blood biomarker tests, Zn2+ was the most toxic with the highest total frequencies of abnormal erythrocytic nucleus. All three metallic ions caused higher abnormal erythrocytic nucleus compared with control groups. In a chronic toxicity test, Pb2+ was the most toxic with lowest growth traits. Survival rate(except for 18 days), total body length and body mass showed significant differences among the treatments. These findings indicated that tadpoles of R. zhenhaiensis should be as a bioindicator of heavy metals pollution. | Li WEI Guohua DING Sainan GUO Meiling TONG Wenjun CHEN Jon FLANDERS Weiwei SHAO Zhihua LIN | 2015 | Asian Herpetological Research2015，6，2： | 3 |
| 3 | How can the Dual-atom Catalyst FeCo-NC Surpass Singleatom Catalysts Fe-NC/Co-NC in CO_(2) RR?-CO Intermediate Assisted Promotion via a Synergistic Effect显示文摘Atomically dispersed catalysts are widely adopted in CO_(2)reduction reaction(CO_(2)RR)due to maximal atomic utilization and high catalytic activity.Dual-atom catalysts(DACs),with more dispersed active sites and distinct electronic structures compared with single-atom catalysts(SACs),may exhibit diverse catalytic performance.Herein,the DAC FeCo-NC and SAC Fe-NC/Co-NC are employed as probes to explore DACs advantage in CO_(2)RR.Results show that the moderate interaction between the dual-atom center and N coordination balances structural stability and catalytic activity.CO is the only product on Fe-NC/Co-NC,and the high limiting potentials from−1.22 to−1.67 V inhibit further reduction.FeCo-NC assisted with CO intermediate exhibits low limiting potentials of−0.64 V for both CH_(3)OH and CH 4,comparable to those on Cu-based catalysts.Under circumstance of applied potentials,CO_(2)RR on FeCo-NC has greater advantages in yielding CH_(3)OH and CH 4 than that on Fe-NC/Co-NC,and hydrogen evolution reaction is severely inhibited.The intrinsic essence is that dual-atom center can provide large spin-polarization and multi-electron transfer capability,rendering CO intermediates as effective electronic and geometric modifiers in CO_(2)RR.This work highlights FeCo-NC as a high-performance CO_(2)RR catalyst toward deep-reduction C1 products and elucidates CO intermediate assisted promotion mechanism via a dual-atom synergistic effect. | Shoufu Cao Sainan Zhou Hongyu Chen Shuxian Wei Siyuan Liu Xiaojing Lin Xiaodong Chen Zhaojie Wang Wenyue Guo Xiaoqing Lu | 2023 | Energy & Environmental Materials2023，6，1： | 1 |
| 4 | A study of emissions and marker gases from smouldering combustion in Larix gmelinii plantations of the Daxing'an Mountains显示文摘Underground fires are characterized by smouldering combustion with a slow rate of spread rate and without flames.Although smouldering combustion releases large amounts of gaseous pollutants,it is difficult to discover by today's forest fire monitoring technologies.Carbon monoxide(CO),nitrogen oxides(NO_(x))and sulfur dioxide(SO_(2))were identified as high concentration marker gases of smouldering combustion-easily-be monitored.According to a two-way ANOVA,combustion time had a significant impact on CO and NO_(x) emissions;smoldering-depth also had a significant impact on NO_(x) emissions but not on CO emissions.Gas emission equations were established by multiple linear regression,C_(co)=156.989-16.626 t and C_(NOx)=3.637-0.252 t-0.039 h. | Shuyuan Tang Sainan Yin Yanlong Shan Bo Gao Long Sun Xiyue Han Mingxia Wang Mingyu Wang Zongshi Chen | 2022 | Journal of Forestry Research2022，33，1： | 1 |
| 5 | Qi-Shen-Yi-Qi Dripping Pills for the Secondary Prevention of Myocardial Infarction: A Randomised Clinical Trial显示文摘 | Hongcai Shang Junhua Zhang Chen Yao Baoyan Liu Xiumei Gao Ming Ren Hongbao Cao Guohua Dai Weiliang Weng Sainan Zhu Hui Wang Hongjuan Xu Boli Zhang Hao Xu | 2013 | Evidence-Based Complementary and Alternative Medicine2013，，： | 1 |
| 6 | Risk factors for atypical endometrial hyperplasia in infertile women:possible association with polycystic ovary syndrome显示文摘Objective:Endometrial hyperplasia is considered as a precursor of endometrial carcinoma,in which oncogenic potential is low in hyperplasia without atypia,but high in atypical hyperplasia.The objective of this study was to identify the risk factors for atypical endometrial hyperplasia in infertile women.Methods:Fifty four infertile women with endometrial hyperplasia,which were selected from a large cohort of 2 098 women who desired for the future childbearing in our center,were diagnosed by hysteroscopy with directed biopsies or dilation and curettage (D&C),including 44 with hyperplasia without atypia,10 with atypical hyperplasia.Clinical characteristics were recorded in terms of age,body mass index (BMI),parity,insulin resistance,polycystic ovary syndrome (PCOS).Statistical comparison was made between women with hyperplasia without atypia and atypical hyperplasia.Logistic regression analysis was employed to assess the contribution of PCOS,obesity and insulin resistance to atypical hyperplasia.Results:The incidence of endometrial hyperplasia in infertile women was 2.57% (54/2098),which included 10 women (0.48%) were diagnosed as atypical hyperplasia.PCOS in women with atypical hyperplasia (70%,7/10) was significantly higher than those of hyperplasia without atypia (27.27%,12/44).Stepwise regression analysis showed that PCOS contributed maximally to atypical endometrial hyperplasia in infertile women.Conclusion:PCOS is an independent risk factor for atypical endometrial hyperplasia in women with infertility.The infertile women with PCOS are at an increased risk for atypical endometrial hyperplasia and endometrial cancer. | Lu Qun Shen Huan Tian Li Zhu Sainan Chen Xi | 2008 | 现代妇产科进展2008，17，2： | 0 |
| 7 | Chemotransformation of Bacterial Cells Without Heat-shock显示文摘Transformation of DNA into microbial cells via heat-shock approach has been well established in the fieldof molecular biology for decades. Herein we described an unexpected finding that heat-shock may not play an essen-tial role in the transformation process. This observation was verified via UV-Vis and fluorescence spectroscopies, andconfocal microscopy images for various DNAs and bacterial cells. The non-heat-shock approach proposed in thisstudy can be a convenient and beneficial modification for DNA transformation, especially for those laboratorieslacking ice machine and heat-shock equipment. | WANG Sainan LI Zhenhua CHEN Zhijun | 2017 | Chemical Research in Chinese Universities2017，33，2： | 0 |
| 8 | Expression and Purification of Human Coagulation Factor X in Mammalian CHO-DG44 Cells显示文摘[Objectives]This study was conducted to obtain a Chinese hamster ovary cell line that stably expresses recombinant human coagulation factor X(rhFX),and to induce efficient expression of the target gene with different concentrations of methotrexate(MTX).[Methods]PCR was performed to obtain the rhFX gene,and a recombinant expression plasmid pOptiVEC-rhFX was constructed and subjected to double restriction endonuclease digestion and sequencing identification.CHO-DG44(DHFR-)cells were transfected by the liposome method,and the target protein was purified by affinity chromatography and detected by SDS-PAGE electrophoresis and Western blot.A cell line with efficient and stable expression of the target gene was obtained by increasing the concentration of MTX to select positive clones.[Results]PCR yielded a 1509 bp rhFX sequence,and the results of double digestion and sequencing showed that the constructed pOptiVEC-rhFX plasmid was correct.After transfection of cells,MTX significantly increased protein expression.When MTX reached 1.0μmol/L,the expression efficiency of the target protein was(9±0.27)μg/ml.The purity of the target protein purified by affinity chromatography was 93%,which could be used for subsequent experiments.The expression efficiency of rhFX in eukaryotic mammalian cells was improved by increasing MTX concentration,and an affinity chromatography purification process for the target protein was preliminarily established.[Conclusions]The results of this study provide data support for the expression and purification of rhFX,and will lay a solid foundation for the development of drugs related to rhFX. | Jinwu CHEN Yi LI Mei LIU Sainan WANG Zilong XIAO Junjie XIA Lulu QI | 2023 | Agricultural Biotechnology2023，12，3： | 0 |
| 9 | Biomolecule@COF:Natural-artificial hybrid microcapsules for controllable biocatalysis显示文摘The assembly of biomacromole particles with artificial solid matrixes to fabricate hybrid composites has been demonstrated as an efficient strategy to promote both components'applications.Elaborating matrixes as well as appropriate assembly method is essential for optimal performances of the obtained natural-artificial composites.Herein,we fabricated the Cyt c@COF-42-B microcapsule through a sacrificial templating method and researched their adjustable biocatalysis by precisely adjusting the microenvironments of these assembled enzyme@COF particles.As a result,the hydrophobicity and mass transfer of the COF microcapsule can be controlled by modifying the different amounts of PEG moieties,thus improving the composite's bioactivity.We found that compared with pristine Cyt c@COF-42-B without PEG,the Cyt c@COF-42-B-PEG exhibited a~2.2 times higher activity.This study provides a significant step toward designing customizable functional natural-artificial composites for enhanced biomacromolecule's activity. | Shan Qiao Mingmin Li Jiangyue Yu Sainan Zhang Dong Yan Zhenjie Zhang Yao Chen | 2022 | Particuology2022，20，5： | 0 |
| 10 | Protein@PP-Zn nanocomplex assembled by coordination of zinc ions used for intracellular protein delivery显示文摘In recent years,intracellular delivery of protein drugs has attracted great attention,and polymer-based systems have been extensively exploited to develop efficient and safe carriers.However,efficient intracellular delivery of protein drugs remains a challenge because of the cell membrane barrier and endosome entrapment.Herein,we report a protein@PP-Zn nanocomplex,which consists of an imidazole-containing block polymer poly(ethylene glycol)-block-poly(β-amino ester)(PEG-b-PAE(Im),PP),zinc ions,and protein drugs,for efficient intracellular protein delivery.PEG-b-PAE(Im)could conjugate proteins via the bridging effect of zinc ions which simultaneously coordinate with imidazole groups on polymer and electron donor groups,such as imidazole and primary amine groups,on protein to improve the loading stability of proteins.Under a slightly acidic environment near cancer cells,the protonation of PAE(Im)backbone increases the positive charge density of the nanocomplex and promotes endocytosis.While under a more acidic environment in endosomes,further protonation of imidazole groups leads to the disintegration of the nanocomplex and the breakdown of endosomes because of the proton sponge effect.Finally,protein is released into the cytoplasm.With the assistance of the nanocomplex,proteins with different sizes and isoelectric points are effectively delivered into cells.This work provides a stable,efficient and universal strategy for intracellular protein delivery. | Liyun Cui Sainan Liu Fan Wu Hua Chen Yuanfeng Li Linqi Shi Yong Liu Rujiang Ma | 2023 | Science China Chemistry2023，66，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员