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| 1 | Clinical features and treatment status of hemifacial spasm in China显示文摘 | Wang Lin Hu Xingyue Dong Hongjuan Wang Wenzhao Huang Yue Jin Lingjing Luo Yumin Zhang Weixi Lian Yajun Liang Zhanhua Shang Huifang Feng Yabo wu Yiwen Chen Jun Luo Weifeng Wan Xinhua | 2014 | Chinese Medical Journal2014，，5： | 20 |
| 2 | Genetic diversity in Chinese modern wheat varieties revealed by microsatellite markers显示文摘Genetic diversity of 1680 modern varieties in Chinese candidate core collections was analyzed at 78 SSR loci by fluorescence detection system. A total of 1336 alleles were detected, of which 1253 alleles could be annotated into 71 loci. For these 71 loci, the alleles ranged from 4 to 44 with an average of 17.6, and the PIC values changed from 0.19 to 0.89 with an average of 0.69. (1) In the three genomes of wheat, the average genetic richness was B>A>D, and the genetic diversity indexes were B>D>A. (2) Among the seven homoeologous groups, the average genetic richness was 2=7>3>4>6>5>1, and the genetic diversity indexes were 7>3>2>4>6>5>1. As a whole, group 7 possessed the highest genetic diversity, while groups 1 and 5 were the lowest. (3) In the 21 wheat chromosomes, 7A, 3B and 2D possessed much higher genetic diversity, while 2A, 1B, 4D, 5D and 1D were the lowest. (4) The highest average genetic diversity index existed in varieties bred in the 1950s, and then it declined continually. However, the change tendency of genetic diversity among decades was not greatly sharp. This was further illustrated by changes of the average genetic distance between varieties. In the 1950s it was the largest (0.731). Since the 1960s, it has decreased gradually (0.711, 0.706, 0.696, 0.695). The genetic base of modern varieties is becoming narrower and narrower. This should be given enough attention by breeders and policy makers. | HAO Chenyang, WANG Lanfen, ZHANG Xueyong, YOU Guangxia, DONG Yushen, JIA Jizeng, LIU Xu, SHANG Xunwu, LIU Sancai & CAO Yongsheng Key Laboratory of Crop Germplasm & Biotechnology, MOA, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China Agronomy College, Gansu Agricultural University, Lanzhou 730070, China | 2006 | Science China(Life Sciences)2006，49，3： | 19 |
| 3 | Nonlinear Adaptive Robust Force Control of Hydraulic Load Simulator显示文摘This paper deals with the high performance force control of hydraulic load simulator. Many previous works for hydraulic force control are based on their linearization equations, but hydraulic inherent nonlinear properties and uncertainties make the conventional feedback proportional-integral-derivative control not yield to high-performance requirements. In this paper, a nonlinear system model is derived and linear parameterization is made for adaptive control. Then a discontinuous projection-based nonlinear adaptive robust force controller is developed for hydraulic load simulator. The proposed controller constructs an asymptotically stable adaptive controller and adaptation laws, which can compensate for the system nonlinearities and uncertain parameters. Meanwhile a well-designed robust controller is also developed to cope with the hydraulic system uncertain nonlinearities. The controller achieves a guaranteed transient performance and final tracking accuracy in the presence of both parametric uncertainties and uncertain nonlinearities; in the absence of uncertain nonlinearities, the scheme also achieves asymptotic tracking performance. Simulation and experiment comparative results are obtained to verify the high-performance nature of the proposed control strategy and the tracking accuracy is greatly improved. | YAO Jianyong JIAO Zongxia YAO Bin SHANG Yaoxing DONG Wenbin | 2012 | Chinese Journal of Aeronautics2012，25，5： | 17 |
| 4 | Effect of advanced age on plasma homocysteine levels and its association with ischemic stroke in non-valvular atrial fibrillation显示文摘BackgroundElevated homocysteine (Hcy ) 被报导了在 atrial 与心血管的事件被联系纤维性颤动(AF ) 病人,当在 AF 的血浆 Hcy 的年龄相关的表示模式仍然保持未知时。学习被瞄准与非瓣膜的 AF 在 2562 个连续病人全部的非瓣膜的 AF patients.MethodsA 与 ischemic 击在血浆 Hcy 层次和它的协会上调查先进年龄的效果, 535 控制被注册并且把年龄组划分了成六。血浆 Hcy 层次在不同年龄组之中被分析,并且 Hcy 上的先进年龄的效果是 investigated.ResultsPlasma Hcy 层次没证明在组之中的任何差别在 65 年下面变老,当它 65-74 年并且超过 75 年在病人严厉地增加了时(15. | Yan YAO Li-Jian GAO Yong ZHOU Jing-Hua ZHAO Qiang LV Jian-Zeng DONG Mei-Sheng SHANG | 2017 | Journal of Geriatric Cardiology2017，14，12： | 13 |
| 5 | Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents,β-ionone and linalool显示文摘Sweet osmanthus(Osmanthus fragrans Lour.)is among the top ten most well-known flowers in China and is recognized as both an aromatic plant and ornamental flower.Here,manual sectioning,scanning electron microscopy,and transmission electron microscopy of sweet osmanthus petals revealed that large amounts of lipids are present inside the petal cells and on the cell surfaces.However,no secretory structures were observed.Instead,the petal cells protrude slightly outward,and the surfaces of the cells are adorned with highly regular brush-shaped hairs.The surfaces of the‘Yingui’petals possessed mostly curled and more numerous hairs,whereas the‘Dangui’petals possessed fewer brush-shaped and more sparsely arranged hairs.In addition,many granular substances were attached to the brush-shaped hairs,and the granules were denser on the hairs of the‘Yingui’petals compared to the hairs on the‘Dangui’petals.Furthermore,35 aromatic components in the‘Yingui’petals and 30 aromatic components in the‘Dangui’petals were detected via GC-MS.The main aromatic component of the‘Yingui’petals wasβ-ionone,whereas that of the‘Dangui’petals was linalool and its oxides.Transcriptome sequencing and qRT-PCR indicated that the highβ-ionone content in the‘Yingui’petals was due to the overexpression of CCD1 and CCD4 and that the high linalool content in the‘Dangui’petals was due to the overexpression of MECS,HDR,IDI1,and LIS1,which function upstream of the linalool synthetic pathway.In particular,the expression levels of CCD4 and LIS1 were upregulated by 5.5-and 5.1-fold in the‘Yingui’and‘Dangui’petals,respectively.One transcription factor(ERF61)was cloned and named,and the expression pattern of ERF61 in sweet osmanthus petals was found to be generally consistent with that of CCD4.Tobacco transformation experiments,yeast one-hybrid experiments,and electrophoretic mobility shift assays indicated that ERF61 binds to the CCD4 promoter and stimulates CCD4 expression,thereby regulating the synthesis ofβ-ionone in sweet osmanthus petals. | Yuanji Han Hongyun Wang Xiaodan Wang Ke Li Meifang Dong Yong Li Qian Zhu Fude Shang | 2019 | Horticulture Research2019，6，1： | 13 |
| 6 | 一种仿生水下机器人的设计与动力学分析(英文)显示文摘A biomimetic underwater vehicle propelled by two undulating long-fins is introduced in this paper. The concerned vehicle is propelled by two symmetrical undulating long-fins installed on both sides. Ten servo motors are employed to drive the long-fins and cosine wave function is employed for motor control. A real-time control system is designed for controlling the long-fins by adjusting its oscillating frequency and oscillating amplitude. An inertial measurement unit is installed to collect the accelerations and angular velocity. To obtain the relationship between oscillating frequency/amplitude and swimming performance, kinematic analysis and hydromechanic analysis are given. By dividing the long-fin into many small elements and computing the hydrodynamic force acting on each element, the instantaneous thrust generated by the long-fin is obtained. Then the average thrust of the long-fin is obtained by summing up the forces acted on the elements in one undulating period. Then swimming experiments are carried out to validate the vehicle design and kinematic analysis and hydromechanic analysis. And two swimming motion modes including marching and rotating locomotion are chosen. Finally, discussions between the swimming performance and the oscillating parameters are given. | WEI Qing-Ping WANG Shuo DONG Xiang SHANG Liu-Ji TAN Min | 2013 | 自动化学报2013，39，8： | 11 |
| 7 | Xiaowei, a New Rice Germplasm for Large-Scale Indoor Research显示文摘Dear Editor,Rice (Oryza sativa)is a model monocot plant for biological studies due to its relatively small genome,rich germplasm resources,and high-efficiency transformation methods.Although significant progress has been made in rice genomics and functional genomics research (Li et al.,2018),large-scale indoor research toward better understanding of rice biology is hampered by its long growth period and extreme dependence on the natural environment for cultivation.There are some rice germplasms that have a short growth period and short plant height,such as Kitaake. | Shikai Hu Xingming Hu Jiang Hu Lianguang Shang Guojun Dong Dali Zeng Longbiao Guo Qian Qian | 2018 | Molecular Plant2018，11，11： | 10 |
| 8 | The High Energy X-ray telescope (HE) onboard the Insight-HXMT astronomy satellite显示文摘The Insight-Hard X-ray Modulation Telescope(Insight-HXMT) is a broadband X-ray and γ-ray(1-3000 ke V) astronomy satellite. One of its three main telescopes is the High Energy X-ray telescope(HE). The main detector plane of HE comprises 18 Na I(Tl)/Cs I(Na) phoswich detectors, where Na I(Tl) is used as the primary detector to measure ~ 20-250 ke V photons incident from the field of view(FOV) defined by collimators, and Cs I(Na) is used as the active shielding detector to Na I(Tl) by pulse shape discrimination. Additionally, Cs I(Na) is used as an omnidirectional γ-ray monitor. The HE collimators have a diverse FOV,i.e. 1.1°×5.7°(15 units), 5.7°×5.7°(2 units), and blocked(1 unit). Therefore, the combined FOV of HE is approximately5.7°×5.7°. Each HE detector has a diameter of 190 mm resulting in a total geometrical area of approximately 5100 cm2, and the energy resolution is ~15% at 60 ke V. For each recorded X-ray event by HE, the timing accuracy is less than 10 μs and the deadtime is less than 10 μs. HE is used for observing spectra and temporal variability of X-ray sources in the 20-250 ke V band either by pointing observations for known sources or scanning observations to unveil new sources. Additionally, HE is used for monitoring the γ-ray burst in 0.2-3 Me V band. This paper not only presents the design and performance of HE instruments but also reports results of the on-ground calibration experiments. | CongZhan Liu YiFei Zhang XuFang Li XueFeng Lu Zhi Chang ZhengWei Li AiMei Zhang YongJie Jin HuiMing Yu Zhao Zhang MinXue Fu YiBao Chen JianFeng Ji YuPeng Xu JingKang Deng RenCheng Shang GuoQing Liu FangJun Lu ShuangNan Zhang YongWei Dong TiPei Li Mei Wu YanGuo Li HuanYu Wang BoBing Wu YongJie Zhang Zhi Zhang ShaoLin Xiong Yuan Liu Shu Zhang HongWei Liu YiJung Yang Fan Zhang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 9 |
| 9 | Global Existence of Solutions for the Cauchy Problem of the Kawahara Equation with L^2 Initial Data显示文摘在这份报纸,我们与 L 2 起始的数据学习 Kawahara 方程的 Cauchy 问题的解决之可能性。由研究 Bourgain 空间 X r, s (R 2 ) 与这个方程联系了,如果,我们证明 Kawahara 方程的 Cauchy 问题是局部地可解决的起始的数据属于 H r (R) 并且 ? 1 < r 鈮 ? 0。如果,这结果与全球答案存在的 Kawahara 方程收益的精力能量守恒定律结合了起始的数据属于 L 2 (R) 。关键词 Kawahara 方程 - Cauchy 问题 - 全球答案先生(2000 ) 题目分类 35Q53 - 中国自然科学基础支持的 35Q35 工程(同意 10171111, 10171112 ) | Shang Bin CUI Dong Gao DENG Shuang Ping TAO | 2006 | Acta Mathematica Sinica,English Series2006，22，5： | 9 |
| 10 | Expert consensus on the glycemic management of critically ill patients显示文摘Introduction The incidence of hyperglycemia is 40-60%in critically ill patients and is up to 60-80%in those who have undergone car-diac surgery.[1]The results of an epidemiological study in the United States showed that 28.6%of diabetic patients and 9.3%of non-diabetic patients had elevated mean daily glucose on the day of ICU admission.[2]In critically ill patients,elevated blood glucose is primarily the result of stress,and stress-induced hy-perglycemia is an independent risk factor associated with prog-nosis,regardless of a previous diagnosis of diabetes.Nutritional therapy has become an integral treatment option for patients in the ICU,[3,4]though nearly 30%of patients with enteral nu-trition and 44-50%with parenteral nutrition(PN)experience elevated glucose.[5,6]Intensive insulin therapy(IIT)is an impor-tant treatment for controlling hyperglycemia in critically ill pa-tients,but it also carries a corresponding risk of hypoglycemia. | Zhixiong Wu Jiao Liu Dong Zhang Kai Kang Xiangrong Zuo Qianghong Xu Aijun Pan Wei Fang Fen Liu You Shang Haiyan Yin Juntao Hu Jinglun Liu Jiangquan Fu Wei Zhang Yuan Zong Min Shao Feng Zhao Mei Meng Yanfei Mao Yingchuan Li Dechang Chen | 2022 | Journal of Intensive Medicine2022，2，3： | 9 |
| 11 | Efficacy and safety of vitamin D3 in patients with diabetic nephropathy: a meta-analysis of randomized controlled trials显示文摘 | Zhao Junyu Dong Jianjun Wang Haipeng Shang Hongxia Zhang Dongmei Liao Lin | 2014 | Chinese Medical Journal2014，，15： | 9 |
| 12 | Relationship between composite structures and compressive properties in CuZr-based bulk metallic glass system显示文摘Bulk metallic glass (BMG) composites with the austenite B2 phase as reinforcement macroscopically showed strain hardening behavior due to the plasticity induced by martensitic transformation during deformation. Relationship between characteristics of the B2-CuZr reinforcing phase and uniaxial compressive properties of CuZr-based BMG composites was studied. Mechanical properties of these BMG composites were found to depend on not only the reinforced phases but also the amorphous matrix,and the yield and fracture strength can be roughly estimated by the rule of mixture principle. Distribution of the reinforced B2-CuZr phase has an important impact on the compressive plasticity even for the composites with a similar volume fraction of the crystalline phase. | WU Yuan SONG WenLi ZHANG ZhongYan HUI XiDong MA Dong WANG XunLi SHANG XinCun LU ZhaoPing | 2011 | Chinese Science Bulletin2011，56，36： | 8 |
| 13 | Microbial diversity accumulates in a downstream direction in the Three Gorges Reservoir显示文摘Organic and inorganic materials migrate downstream and have important roles in regulating environmental health in the river networks.However,it remains unclear whether and how a mixture of materials(i.e.,microbial species)from various upstream habitats contribute to microbial community coalescence upstream of a dam.Here we track the spatial variation in microbial abundance and diversity in the Three Gorges Reservoir based on quantitative PCR and 16 S rRNA gene high-throughput sequencing data.We further quantitatively assess the relative contributions of microbial species from mainstem,its tributaries,and the surrounding riverbank soils to the area immediately upstream of the Three Gorges Dam(TGD).We found an increase of microbial diversity and the convergent microbial distribution pattern in areas immediately upstream of TGD,suggesting this area become a new confluence for microbial diversity immigrating from upstream.Indeed,the number of shared species increased from upstream to TGD but unique species decreased,indicating immigration of various sources of microbial species overwhelms local environmental conditions in structuring microbial community close to TGD.By quantifying the sources of microbial species close to TGD,we found little contribution from soils as compared to tributaries,especially for sites closer to TGD,suggesting tributary microbes have greater influence on microbial diversity and environmental health in the Three Gorges Reservoir.Collectively,our results suggest that tracking microbial geographic origin and evaluating accumulating effects of microbial diversity shed light on the ecological processes in microbial communities and provide information for regulating aquatic ecological health. | Shang Wang Weiguo Hou Hongchen Jiang Liuqin Huang Hailiang Dong Shu Chen Bin Wang Yongcan Chen Binliang Lin Ye Deng | 2021 | Journal of Environmental Sciences2021，33，3： | 7 |
| 14 | Genomic insights into the fast growth of paulownias and the formation of Paulownia witches' broom显示文摘Paulownias are among the fastest growing trees in the world,but they often suffer tremendous loss of wood production due to infection by Paulownia witches'broom(PaWB)phytoplasmas.In this study,we have sequenced and assembled a high-quality nuclear genome of Paulownia fortunei,a commonly cultivated paulownia species.The assembled genome of P.fortunei is 511.6 Mb in size,with 93.2%of its sequences anchored to 20 pseudo-chromosomes,and it contains 31985 protein-coding genes.Phylogenomic analyses show that the family Paulowniaceae is sister to a clade composed of Phrymaceae and Orobanchaceae.Higher photosynthetic efficiency is achieved by integrating C3 photosynthesis and the crassulacean acid metabolism pathway,which may contribute to the extremely fast growth habit of paulownia trees.Comparative transcriptome analyses reveal modules related to cambial growth and development,photosynthesis,and defense responses.Additional genome sequencing of PaWB phytoplasma,combined with functional analyses,indicates that the effector PaWB-SAP54 interacts directly with Paulownia PfSPLa,which in turn causes the degradation of PfSPLa by the ubiquitin-mediated pathway and leads to the formation of witches'broom.Taken together,these results provide significant insights into the biology of paulownias and the regulatory mechanism for the formation of PaWB. | Yabing Cao Guiling Sun Xiaoqiao Zhai Pingluo Xu Liming Ma Minjie Deng Zhenli Zhao Haibo Yang Yanpeng Dong Zhonghai Shang Yujie Lv Lijun Yan Haifang Liu Xibing Cao Bingbing Li Zhe Wang Xiaogai Zhao Haiyan Yu Fan Wang Wen Ma Jinling Huang Guoqiang Fan | 2021 | Molecular Plant2021，14，10： | 7 |
| 15 | High-effciency aircraft antiskid brake control algorithm via runway condition identification based on an on-off valve array显示文摘The aircraft antiskid braking system is an important hydraulic system for preventing tire bursts and ensuring safe take-off and landing. The brake system adjusts the force applied on the brake discs by controlling the brake pressure. Traditional aircraft antiskid braking systems achieve antiskid performance by controlling the braking pressure with an electrohydraulic servo valve.Because the pilot stage of an electrohydraulic servo valve is easily blocked by carbonized hydraulic oil, the servo valve would become a dangerous weak point for aircraft safety. This paper proposes a new approach that uses an on-off valve array to replace the servo valve for pressure control. Based on this new pressure control component, an efficient antiskid control algorithm that can utilize this discontinuous feature is proposed. Furthermore, the algorithm has the ability to identify the runway circumstances. To overcome the discontinuity in the process of using an on-off valve array, the Filippov framework is introduced. The conditions of convergence of the system are also discussed.The results of the digital simulations and the hardware-in-the-loop(HIL) braking experiments are used to verify the efficiency and stability of the proposed control algorithm. The method also proves that the on-off valve array can replace the servo valve perfectly as a new type of antiskid braking pressure control component. | Dong SUN Zongxia JIAO Yaoxing SHANG Shuai WU Xiaochao LIU | 2019 | Chinese Journal of Aeronautics2019，32，11： | 7 |
| 16 | Evolution and molecular epidemiology of foot-and-mouth disease virus in China显示文摘Foot-and-mouth disease (FMD),caused by foot-and-mouth disease virus (FMDV),is considered one of the most important viral diseases of cloven-hoofed animals,causing severe economic losses in affected regions of the world.Three serotypes (A,O,and Asia 1) of FMDV have been identified in China since 1958.In addition,the occurrence of novel subtypes within these serotypes has made the epidemiology of FMDV more complicated over the last few years.In this review,we summarize the history and the current epidemiological situation in China,genetic diversity (e.g.,quasispecies dynamics,antigenic heterogeneity,and functional constraints),intertypic recombination,and the evidence for positive selection of different FMDV serotypes.We also assess these genetic data to understand the origin,evolution,and transmission of FMDV,the findings of which may be useful in developing control measures for future epidemics. | BAI XingWen LI PingHua BAO HuiFang LIU ZaiXin LI Dong LU ZengJun CAO YiMei SHANG YouJun SHAO JunJun CHANG HuiYun LUO JianXun LIU XiangTao | 2011 | Chinese Science Bulletin2011，56，21： | 7 |
| 17 | Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals显示文摘Previous studies have shown that high light intensity can induce anthocyanin synthesis(AS)in petunia plants.To identifywhich kind of light quality plays a role in inducing such metabolic process,and what transcripts participate in controlling it,we carried out whole-transcriptome sequencing and analysis of petunia petals treated with different light-quality conditions.Among the red and white light treatments,a total of 2205 differentially expressed genes and 15,22,and 20 differentially expressed circRNAs,miRNAs,and lncRNAs,were identified respectively.The AS-related genes,including the structural genes CHSj,F3H,F35H,DFR,and ANS,and the regulatory genes AN4,DPL,PHZ and MYBx were found to be downregulated under red light condition compared with their levels under white light condition.Furthermore,the light photoreceptor Cryptochrome 3(CRY3)and a series of light-dependent genes,such as PIF,HY5,andBBXs,were also determined to respond to the light treatments.The anthocyanin contents in early petunia petals under red light were significantly lower than that under white and blue light.The results of qRT-PCR further confirmed the expression pattern of some AS-related and light-response genes in response to different light quality.Yeast two-hybrid results showed that the key elements in the light signal pathway,HY5 can interact with BBX19,BBX24 and BBX25.And PHZ,the important AS regulator can induce anthocyanin synthesis in response to blue light quality fromtransient expression analysis in petunia petals.These findings presented here not only deepen our understanding of how light quality controls anthocyanin synthesis,but also allow us to explore potential target genes for improving pigment production in petunia flower petals. | Zhenzhu Fu Hongquan Shang Hui Jiang Jie Gao Xiaoyu Dong Huijuan Wang Yanmin Li Limin Wang Jing Zhang Qingyan Shu Yacong Chao Menglan Xu Rui Wang Liangsheng Wang Hechen Zhang | 2020 | Horticultural Plant Journal2020，6，6： | 7 |
| 18 | In-situ electrochemical activation designed hybrid electrocatalysts for water electrolysis显示文摘Developing transition metal-based electrocatalysts with rich active sites for water electrolysis plays important roles in renewable energy fields. So far, some strategies including designing nanostructures,incorporating conductive support or foreign elements have been adopted to develop efficient electrocatalysts. Herein, we summarize recent progresses and propose in-situ electrochemical activation as a new pretreating technique for enhanced catalytic performances. The activation techniques mainly comprise facile electrochemical processes such as anodic oxidation, cathodic reduction, etching, lithium-assisted tuning and counter electrode electro-dissolution. During these electrochemical treatments, the catalyst surfaces are modified from bulk phase, which can tune local electronic structures, create more active species, enlarge surface area and thus improve the catalytic performances. Meanwhile, this technique can couple the atomic, electronic structures with electrocatalysis mechanisms for water splitting.Compared to traditional chemical treatment, the in-situ electrochemical activation techniques have superior advantages such as facile operation, mild environment, variable control, high efficiency and flexibility. This review may provide guidance for improving water electrolysis efficiencies and hold promising for application in many other energy-conversion fields such as supercapacitors, fuel cells and batteries. | Xiao Shang Bin Dong Yong-Ming Chai Chen-Guang Liu | 2018 | Science Bulletin2018，63，13： | 6 |
| 19 | Shugan-decoction relieves visceral hyperalgesia and reduces TRPV1 and SP colon expression显示文摘AIM:To evaluate the therapeutic effect of Shugan-decoction(SGD)on visceral hyperalgesia and colon gene expressions using a rat model.METHODS:Ninety-six adult male Wistar rats were randomized into six equal groups for assessment of SGD effects on psychological stress-induced changes using the classic water avoidance stress(WAS)test.Untreated model rats were exposed to chronic(1 h/d for 10 d consecutive)WAS conditions;experimental treatment model rats were administered with intragastric SGD at1 h before WAS on consecutive days 4-10(low-dose:0.1g/mL;mid-dose:0.2 g/mL;high-dose:0.4 g/mL);control treatment model rats were similarly administered with the irritable bowel syndrome drug,dicetel(0.0042g/mL);untreated normal control rats received no drug and were not subjected to the WAS test.At the end of the 10-d WAS testing period,a semi-quantitative measurement of visceral sensitivity was made by assessing the abdominal withdrawal reflex(AWR)to colorectal balloon-induced distension(at 5 mmHg increments)to determine the pain pressure threshold(PPT,evidenced by pain behavior).Subsequently,the animals were sacrificed and colonic tissues collected for assessment of changes in expressions of proteins related to visceral hypersensitivity(transient receptor potential vanilloid 1,TRPV1)and sustained visceral hyperalgesia(substance P,SP)by immunohistochemistry and real-time polymerase chain reaction.Inter-group differences were assessed by paired t test or repeated measures analysis of variance.RESULTS:The WAS test successfully induced visceral hypersensitivity,as evidenced by a significantly reduced AWR pressure in the untreated model group as compared to the untreated normal control group(190.4±3.48 mmHg vs 224.0±4.99 mmHg,P<0.001).SGD treatments at mid-dose and high-dose and the dicetel treatment significantly increased the WAS-reduced PPT(212.5±2.54,216.5±3.50 and 217.7±2.83 mmHg respectively,all P<0.001);however,the low-dose SGD treatment produced no significant effect on the WAS-reduced PPT(198.3±1.78 mmHg,P>0.05).These trends corresponded to the differential expressions observed for both TRPV1 protein(mid-dose:1.64±0.08 and high-dose:1.69±0.12 vs untreated model:3.65±0.32,P<0.001)and mRNA(0.44±0.16 and0.15±0.03 vs 1.39±0.15,P<0.001)and SP protein(0.99±0.20 and 1.03±0.23 vs 2.03±0.12,P<0.01)and mRNA(1.64±0.19 and 1.32±0.14 vs 2.60±0.33,P<0.05).These differential expressions of TRPV1 and SP related to mid-and high-dose SGD treatments were statistically similar to the changes induced by dicetel treatment.No signs of overt damage to the rat system were observed for any of the SGD dosages.CONCLUSION:Shugan-decoction can reduce chronic stress-induced visceral hypersensitivity in rats,and the regulatory mechanism may involve mediating the expressions of TRPV1 and SP in colon tissues. | Jing-Juan Shang Jian-Ye Yuan Hui Xu Rong-Zhu Tang Yue-Bin Dong Jian-Qun Xie | 2013 | World Journal of Gastroenterology2013，19，44： | 6 |
| 20 | Analysis of aristolochic acids, aristololactams and their analogues using liquid chromatography tandem mass spectrometry显示文摘More than 80 aristolochic acids(AAs) and aristololactams(ALs) have been found in plants of the Aristolochiaceae family, but relatively few have been fully studied. The present study aimed at developing and validating a liquid chromatography tandem mass spectrometry(LC/MS^n) for the analysis of these compounds. We characterized the fragmentation behaviors of 31 AAs, ALs, and their analogues via high performance liquid chromatography coupled with electrospray ionization mass spectrometry. We summarized their fragmentation rules and used these rules to identify the constituents contained in Aristolochia contorta, Ar. debilis, Ar. manshurensis, Ar. fangchi, Ar. cinnabarina, and Ar. mollissima. The AAs and ALs showed very different MS behaviors. In MS1 of AAs, the characteristic pseudomolecular ions were [M + NH_4]^+, [M + H]^+, and [M + H - H_2O]^+. However, only [M + H]^+ was found in the MS1 of ALs, which was simpler than that of AAs. Distinct MSn fragmentation patterns were found for AAs and ALs, showing the same skeleton among the different substituent groups. The distribution of the 31 constituents in the 6 species of Aristolochia genus was reported for the first time. 25 Analogues of AAs and ALs were detected in this genus. A hierarchical schemes and a calculating formula of the molecular formula of these nitrophenanthrene carboxylic acids and their lactams were proposed. In conclusion, this method could be applied to identification of similar unknown constituents in other plants. | YU Jie MA Chao-Mei WANG Xuan SHANG Ming-Ying Masao Hattori XU Feng JING Yu DONG Shi-Wen XU Yu-Qiong ZHANG Cui-Ying CAI Shao-Qing | 2016 | Chinese Journal of Natural Medicines2016，14，8： | 6 |
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