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| 1 | Methionine-dependence and combination chemotherapy on human gastric cancer cells in vitro显示文摘AIM: To elucidate whether human primary gastric cancer and gastric mucosa epithelial calls in vitro can grow normally in a rnethionine (Met) depleted environment, i.e.Met-dependence, and whether Met-depleting status can enhance the killing effect of chemotherapy on gastric cancer cells.lMETHODS: Fresh human gastric cancer and mucosal tissueswere managed to form monocellular suspensions, whichwere then cultured in the Met-free but homocysteine-containing ( MetHcy+ ) medium, with differentchemotherapeutic drugs. The proliferation of the cells wasexamined by cell counter, flow cytometry (FCM) andmicrocytotoxicity assay (MTT).RESULTS: The growth of human primary gastric cancer cellsin Met Hcy+ was suppressed, manifested by the decrease oftotal cell counts [1.46±0.42 ( x 109@L-1) in Met-Hcy+ vs .64±0.44 ( x l09@L-1) in Met+ Hcy, P<0.01], the decline inthe percentage of G0G1 phase cells (0.69±0.24 in Met-Hey+vs 0.80±0.18 in Met+ Hcy, P<0.01) and the increase of Scells(0.24±0.20inMet-Hcy+ vs 0.17 ± 0.16 in Met+ Hcy-, P< 0.01); however, gastric mucusal cells grew normally. IfMet-Hcy+ medium was used in combination withchemotherapeutic drugs, the number of surviving gastriccancer cells dropped significantly.CONCLUSION: Human primary gastric cancer cells in vitroare Met-dependent; however, gastric mucosal cells have notshown the same characteristica. Met- Hcy+ environment maystrengthen the killing effect of chemotherapy on humanprimary gastric cancer cells. | Wei-Xin Cao Jing-Min Ou Xu-Feng Fei,Department of Clinical Nutrition,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Zheng-Gang Zhu Hao-Ran Yin Min Yan Yan-Zhen Lin,Department of Surgery,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Second Medical University,Shangha 200025,China | 2002 | World Journal of Gastroenterology2002，8，2： | 24 |
| 2 | Nerve Growth Factor Prevents the Apoptosis-associated Increase in Acetylcholinesterase Activity after Hydrogen Peroxide Treatment by Activating Akt显示文摘Acetylcholinesterase (疼痛) 被认为玩结果显示出的重要角色 duringapoptosis.Our 那 H_2O_2 导致了疼痛活动,功能的标记 inapoptosis,象 neuronal 一样 PC 12 房间的增加。谷胱甘肽,涉及细胞的氧化还原作用动态平衡,禁止了疼痛活动的增加,建议在这个过程的那个反应的氧种类(ROS ) 千盐湖钥匙角色。进一步的调查证明疼痛的举起在 Akt,从进 cytosol 的线粒体的细胞色素 c 的版本,和 caspase 家庭成员的激活的降级以后被观察。神经生长因素(NGF ) 什么时候是在场的,与 Akt 水平,疼痛的举起,细胞色素 c 散开,以及 apoptosis 的维护,显著地在对待 H_2O_2 的 PC12 细胞被稀释。然而, wortmannin, PI3K/Aktpathway 的一个禁止者,加速了 apoptosis 并且增加了疼痛活动。过去表示 ofconstitutively 激活 Akt,它是 NGF receptorTrkA,推迟的 mitochondrial 倒塌和疼痛活动的禁止的举起的一个下游的发信号元素。因此, NGF preventedapoptosis 和由激活 Akt 小径并且稳定线粒体的功能的疼痛活动的举起。 | Hua JIANG~(1,3) Jingya ZHANG~(1,3) Hui ZHU~(1,3) Hong LI~2 and Xuejun ZHANG~1 ~1Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Shangha Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China ~2DEFEMA,Faculté de Medecine et de Pharmacie,University of Rouen,Rouen 76821,France ~3Graduate school of the Chinese Academy of Sciences,Beijing 100080,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，1： | 9 |
| 3 | Gankyrin promotes tumor growth and metastasis through activation of IL‐6/STAT3 signaling in human cholangiocarcinoma显示文摘 | Tongsen Zheng Xuehui Hong Jiabei Wang Tiemin Pei Yingjian Liang Dalong Yin Ruipeng Song Xuan Song Zhaoyang Lu Shuyi Qi Jiaren Liu Boshi Sun Changming Xie Shangha Pan Yuejin Li Xiaohe Luo Shuai Li Xiang Fang Nishant Bhatta Hongchi Jiang Lianxin Liu | 2014 | Hepatology2014，，： | 2 |
| 4 | In vitro characterizetion of prostanoid receptors onhuman myometrrium at termpregnancy显示文摘 | Marshall K Shangha R Clayton JK | 1993 | Senior Br J Pharmacol1993，108，2： | 1 |
| 5 | The Strategy of Combined Ischemia Preconditioning and Salvianolic Acid-B Pretreatment to Prevent Hepatic Ischemia-Reperfusion Injury in Rats显示文摘 | Rui Kong Yue Gao Bei Sun Hua Chen Gang Wang Xiuyun Wang Hong Zhu Shangha Pan Dongbo Xue Hongchi Jiang | 2009 | Digestive Diseases and Sciences2009，，12： | 1 |
| 6 | In vitro characterization of prostanoid receptors on human myometrrium at termpregnancy显示文摘 | Marshall K Shangha R Clayton JK | 1993 | Senior Br J Pharmacol1993，108，2： | 1 |
| 7 | Laminaria gametophyte clone culture and its application in sporeling cultivation显示文摘In China, the total annual production of cultured Laminaria japonica reached half million tons in dry weight there few years. The routine sporeling culture technique conducted in the greenhouse took at least three and half months. In such a case, sometimes the sporelings died within a few days due to destructive diseases. In Order to overcome the mentioned problems, a new sporeling culture technique, the clone technique, is developed. The method includes three stead: (1) Gametophyte clone culture. The spores and the gametophytes are cloned in flasks under favorable environments. (2) Sporeling cul ture. Male and female clones are crushed and spread onto a frame to allow the gametophytes to attach to the substrata. The frames are cultured in tanks, and the sporophytes reach 1 cm in length within one and a half months. (3) Outgrowing of the plant. The frames are put in the open sea when seawater temperature decreased to 20℃. After one month, the sporelings are large enough to be transplanted. It is concluded that the clone technique has the following advantages: (1)Large amount of clones can be produced in a short period of time. (2) Clone seeding method makes it free from the biological rhythm, one can seed the plant anytime all the year round. (3) It takes only one and a half months to complete the process of sporeling cultivation in the greenhouse. At present, this technique is used in the breeding of new strains of Laminaria. | Zhou Zhigang1 Li Dapeng2 Wu Chaoyuan2 Liu Haihang2 (1. Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Prasent address: College of Fishery Science, Shanghai Fisheries University, 334 Jungong Road, Shangha 20 | 2000 | Acta Oceanologica Sinica2000，19，2： | 1 |
| 8 | Downregulation of nuclear factor-κB p65 subunit by small interfering RNA synergizes with gemcitabine to inhibit the growth of pancreatic cancer显示文摘 | Rui Kong Bei Sun Hongchi Jiang Shangha Pan Hua Chen Shuangjia Wang Geoffrey W. Krissansen Xueying Sun | 2009 | Cancer Letters2009，，1： | 1 |
| 9 | Upregulation of HIF-2α induced by sorafenib contributes to the resistance by activating the TGF-α/EGFR pathway in hepatocellular carcinoma cells显示文摘 | Dali Zhao Bo Zhai Changjun He Gang Tan Xian Jiang Shangha Pan Xuesong Dong Zheng Wei Lixin Ma Haiquan Qiao Hongchi Jiang Xueying Sun | 2014 | Cellular Signalling2014，，： | 1 |
| 10 | Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway显示文摘 | Gang Wang Bing Han Haoxin Zhou Linfeng Wu Yongwei Wang Guang Jia Jiachen Lv Zhuoxin Cheng Shangha Pan Ji Liu Yinan Zhou Bei Sun | 2013 | Apoptosis2013，，1： | 1 |
| 11 | Upregulation of HIF-2α induced by sorafenib contributes to the resistance by activating the TGF-α/EGFR pathway in hepatocellular carcinoma cells显示文摘 | Dali Zhao Bo Zhai Changjun He Gang Tan Xian Jiang Shangha Pan Xuesong Dong Zheng Wei Lixin Ma Haiquan Qiao Hongchi Jiang Xueying Sun | 2014 | Cellular Signalling2014，，： | 1 |
| 12 | Complete eradication of hepatocellular carcinomas by combined vasostatin gene therapy and B7H3-mediated immunotherapy显示文摘 | Lixin Ma Liqiong Luo Haiquan Qiao Xuesong Dong Shangha Pan Hongchi Jiang Geoffrey W. Krissansen Xueying Sun | 2006 | Journal of Hepatology2006，，： | 1 |
| 13 | Genistein potentiates the effect of arsenic trioxide against human hepatocellular carcinoma: Role of Akt and nuclear factor-κB显示文摘 | Yong Ma Jizhou Wang Lianxin Liu Huaqiang Zhu Xiaoning Chen Shangha Pan Xueying Sun Hongchi Jiang | 2010 | Cancer Letters2010，，1： | 1 |
| 14 | Matrine induces programmed cell death and regulates expression of relevant genes based on PCR array analysis in C6 glioma cells显示文摘 | Shujun Zhang Jiping Qi Libo Sun Binglin Cheng Shangha Pan Min Zhou Xueying Sun | 2009 | Molecular Biology Reports2009，，4： | 1 |
| 15 | IDENTIFICATION OF ALTERNARIOL, ALTERNARIOL MONOMETHYL ETHER AND ZEARALENONE BY PARTICLE BEAM LC/MS显示文摘A method for simultaneously analyzing altemariol(AOH), altemariol monomethyl ether (AME) and zearalenone(ZEA) by particle beam LC/MS was established, LC separation was accompiished with a solvent system of methanol and water (80:20 v/v). The followed particle beam LC/MS analysis gave searchable spectra of AOH. AME and ZEA. Application of this technique to analysis of an alternaria culture confirmed the presence of AOH and AME. | Feng LIU*, Yi LUO and Li Han ZHANG Institute of Pharmacology and Toxicology, 100850 Beijing Kai Yuan KUANG and Shi Ying SHI Institute of Plant and Protection, Academy of Agriculture, 201106 Shangha | 1993 | Chinese Chemical Letters1993，4，11： | 0 |
| 16 | In-situ calibration of acoustic measurements of suspended sediment显示文摘I^IONInseawatertherearevariousedirnentswhichcanaffectmarineenvi^t.Forexample,P8rticularlyinestuaryarea,theto^hyofchannelandseabedisccingcontinuouslybecauseofthedespitionofagreatquantityofmudsandsandsbroughtbyilifluentriver.In^talareasandharassthere-s... | Guo Jijie Ren inifa and Li Yunwu( Institute of Ocean Technology, State Oceanic Administratiod, Tianjin 300ill, China) (Shangha caustics Labortory, Chinese Academy of thences, Shangha 200032, China) | 1999 | Acta Oceanologica Sinica1999，18，4： | 0 |
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