|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | 胡萝卜软腐果胶杆菌巴西亚种(Pectobacterium carotovorum subsp.brasiliense)引起的黄瓜细菌性软腐病在中国发生显示文摘2014—2015年,在中国山东、山西、河北、河南、辽宁等省的黄瓜茎叶上发生了毁灭性的细菌性软腐病,造成了黄瓜产业严重的经济损失。黄瓜叶片、茎、叶柄和果实表面出现流胶;茎基部呈深褐色进而出现湿腐。受侵染的黄瓜叶边缘出现黄斑和湿腐症状,并逐渐扩散至叶片中心。从受侵染组织中分离出45个细菌菌株,在形态学特性鉴定、生理学、生物化学和16Sr RNA基因序列分析的基础上,将病原体鉴定为胡萝卜软腐果胶杆菌(Pectobacterium carotovorum)。多位点序列分析(MLSA)证实,分离菌株是胡萝卜软腐果胶杆菌巴西亚种[Pectobacterium carotovorum subsp.brasiliensis(Pcb)],病原菌归入进化枝II。重新分离获得与原始菌株相同的菌株,证实了分离菌株的致病力。寄主范围测试表明,该菌株具有广泛的寄主范围。目前为止,这是对Pcb引起的黄瓜软腐病——这一对黄瓜生产具有重要经济影响的病害在中国乃至全球范围内的首次报道。 | 冯志琴(译) meng x l chai a l shi y x xie x w ma z h li b j | 2017 | 中国植保导刊2017，37，7： | 10 |
| 4 | Isolation of GIF from porcine brain and studies of its zinc transfer kinetics with apo-carbonic anhydrase显示文摘 | Shi Y B Du L Zheng W J | 2002 | Biometal2002，15，4： | 2 |
| 5 | 纳米光子结构软膜紫外光固化纳米压印(英文)显示文摘紫外光固化纳米压印是实现纳米结构批量复制的一种新技术.其特点是低成本和高分辨,而且可以达到极高的套刻精度.为了得到大面积图案的均匀复制,可用聚二甲基硅氧烷(PDMS)制备透光的压印软模板.其母版图案可由高分辨率电子束曝光和反应离子刻蚀的方法在硅片基底上获得,然后用浇注的方法将图案转移到PDMS上.本实验特别发展了紫外光固化纳米压印适用于软膜压印的双层膜图型转移技术.该双层膜由廉价的光胶和聚甲基丙烯酸甲脂(PMMA)构成.对光胶层的压印可用普通的光学曝光仪实现.然后再将图案用反应离子刻蚀的方法转移到PMMA层中.为了证明方案的可行性,在两种不同材料的半导体基片上压印了三角晶格的光子晶体和准晶结构的图案,并用剥离的方法将它们转移到金属薄膜上,最后成功地进行了硅片刻蚀实验.相信这一纳米制做方法对大面积纳米光子结构和光学集成芯片的制造是普遍适用的. | SHI J WANG Z M BAO K PEROZ C BELOTTI M XU L P LUO C X SUN M H ZHANG B JI H OUYANG Q CHEN Y | 2006 | 纳米技术与精密工程2006，4，3： | 2 |
| 6 | Effect of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and FAS activity 显示文摘 | Zhang C B Teng L Shi Y | 2002 | Chin Med J2002，115，7： | 2 |
| 7 | Bioreactivity of particulate matter in Beijing air: Results from plasmid DNA assay 显示文摘 | Shao L Y Shi Z B Jones T P | 2006 | Scienceof the Total Environment2006，367，： | 1 |
| 8 | Quantitative analysis of monofluoroacetate in biologicalsamples by high-performance liquid chromatography using fluorescence labeling with 9-chloromethylanthracene显示文摘 | Xie Z M Shi W B Liu L | 2007 | J Chromatogr B2007，857，： | 1 |
| 9 | Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry 显示文摘 | Shi B X Wang Y Lam T L | 2010 | Biomicrofluidics2010，4，： | 1 |
| 10 | Flow-based packet-mode load-balancing for paral- lel packet switches 显示文摘 | Shi L Li W J Liu B | 2010 | Journal of High Speed Networks2010，17，2： | 1 |
| 11 | Estimating optical flow with cellular neural networks 显示文摘 | SHI B E ROSKA T CHUA L O | 1998 | International Journal of Circuit Theory And Applications1998，26，4： | 1 |
| 12 | Molecularly Imprinted Polymer Microspheres for Solid-phase Extraction of Chloramphenicol Residues in Foods 显示文摘 | Shi X Z Wu A B Zheng S L | 2007 | J Chromatogr B2007，850，12： | 1 |
| 13 | 显示文摘 | Wang L J Sang W B Shi W M | 2000 | Nucl Instrum Methods Phys Res Sect A2000，448，3： | 1 |
| 14 | Diplatinum alkynyl chromophores as sensitisers for lanthanide It from lanthanides using a in Pt2 Ln2 and Pt2 Ln4 ( Ln = Eu, Nd, :b) arrays with acetylide-functionalized bipyridine/phenanthroline 显示文摘 | XU H B SHI L X MA E | 2006 | Chemical Communications2006，21，15： | 1 |
| 15 | Comparative study of anion polyacrylamide (APAM) adsorption-related fouling o a PVDF UF membrane and a modified PVDF UF membrane 显示文摘 | YiXS Shi W X Yu S L Sun N Jin L M Wang S Zhang B Ma C Sun L P | 2012 | Desalination2012，286，： | 1 |
| 16 | Beneficial effect of galanin on insulin sensitivity in muscle of type 2diabetic rats显示文摘 | He B Shi M Zhang L | 2011 | Physiol Behav2011，103，1： | 1 |
| 17 | Contribution of Primary Care to Health Systems and Health显示文摘 | Startfield B Shi L Macinko J | 2005 | The Milbank Quarterly2005，83，3： | 1 |
| 18 | Dosage effects of EGb761 on hydrogen peroxide-induced cell death in SH-SY5Y cells显示文摘 | Shi C Zhao L Zhu B | 2009 | Chem Biol Interact2009，180，3： | 1 |
| 19 | Nirl promotes in- vasion of breast cancer cells by binding to chemokine( C-C mo- tif) ligand 18 through the PI3K/AM/GSK3/Snall signalling pathway显示文摘 | Zhang B Yin C Li H Shi L Liu N Sun Y | 2013 | Eur J Cancer2013，49，18： | 1 |
| 20 | Grain yield and water use efficiency of two types of winter wheat cultivars under different water regimes显示文摘 | Dong B D Shi L Shi C H | 2011 | Agricultural Water Management2011，99，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员