|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 2 | CsWRKY13,a novel WRKY transcription factor of Camellia sinensis,involved in lignin biosynthesis and accumulation显示文摘Lignin is an aromatic polymer that provides the necessary mechanical strength for the transport of water and nutrients in higher plants.Lignin biosynthesis and accumulation affect growth and development of tea plants.The degree of lignification related to the tenderness of fresh tea leaves determines the quality of tea.WRKY transcription factors play central roles in plant development and physiological processes.However,the roles of WRKY transcription factors in lignin biosynthesis of tea plants remain unclear.In this study,a WRKY gene,CsWRKY13,was cloned from tea plant'Longjing 43'.The open reading frame(ORF)of CsWRKY13 gene was 708 bp,encoding 235 amino acids.Sequence analysis showed that CsWRKY13 contained a conserved WRKYGQK amino acid sequence and a zinc-finger-like motif CX4CX23HXH.Subcellular localization showed that CsWRKY13 was localized in the nucleus.The yeast trans-activation assay showed that CsWRKY13 had no transcriptional activity.Expression analysis showed that the CsWRKY13 gene was highly expressed in the stem.Overexpression of CsWRKY13 in Arabidopsis thaliana reduced lignin content and the expression levels of genes related to lignin biosynthesis in transgenic plants.Most flavonoids pathway related genes were significantly up-regulated.This study shows that CsWRKY13 might function as a negative regulator in regulation of lignin synthesis. | Ruimin Teng Yongxin Wang Shijia Lin Yi Chen Yazhuo Yang Ni Yang Jingwen Li Jing Zhuang | 2021 | Beverage Plant Research2021，1，1： | 1 |
| 3 | Identification of quantitative trait loci for seed storability in rice ( Oryza sativa L.)显示文摘 | LINFANG LI QIUYUN LIN SHIJIA LIU XI LIU WENYAN WANG NGO THI HANG FENG LIU ZHIGANG ZHAO LING JIANG JIANMIN WAN | 2012 | Plant Breeding2012，，6： | 1 |
| 4 | Bioleaching and biosorption behavior of vanadium-bearing stone coal by Bacillus mucilaginosus显示文摘The recovery of vanadium(V)from stone coal by bioleaching is a promising method.The bioleaching experiments and the biosorption experiments were carried out,aiming to explore the adsorption characteristics of Bacillus mucilaginosus(B.mucilaginosus)on the surface of vanadium-bearing stone coal,and the related mechanisms have been investigated.After bioleaching at 30℃ for 28 d,the cumulative leaching rate of V reached 60.2%.The biosorption of B.mucilaginosus on stone coal was affected by many factors.When the pH value of leaching system is 5.0,strong electrostatic attraction between bacteria and stone coal promoted biosorption.Bacteria in the logarithmic growth phase had mature and excellent biosorption properties.The initial bacterial concentration of 3.5×10^(8) CFU/mL was conducive to adhesion,with 38.9%adsorption rate and 3.6×10^(7) CFU/g adsorption quantity.The adsorption of B.mucilaginosus on the stone coal conformed to the Freundlich model and the pseudo-second-order kinetic model.Bacterial surface carried functional groups(-CH_(2),-CH_(3),-NH_(2),etc.),which were highly correlated with the adsorption behavior.In addition,biosorption changed the surface properties of stone coal,resulting in the isoelectric point(IEP)approaching the bacteria.The results could provide an effective reference for the adsorption laws of bacteria on minerals. | Yingbo Dong Shijia Chong Hai Lin | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，2： | 0 |
| 5 | Digestion performance improvement of tributyrin through nano-emulsion preparation technology显示文摘The current research aimed to optimize the preparation technology of tributyrin nano-emulsion(TBNE)to improve its digestion properties, which were investigated through digestion models in vitro and in vivo. The content of each component of TBNE was optimized by response surface methodology(RSM) to improve the z-average particle size and stability of TBNE. The optimized TBNE was evaluated for its digestion properties by in vitro and in vivo models. The preparation conditions of TBNE optimized by RSM were as follows: 61.3% of sorbitol, 32.7% of tributyrin, and 6.0% of modified phospholipid(MP). The predicted z-average particle size of TBNE was(246.02 ± 18.10) nm. The results of the verification test showed that the z-average particle size, zeta potential, conductivity, emulsification activity index, and emulsification stability index of TBNE were(250.02 ± 7.18) nm,(-40.23 ± 0.76) m V,(31.80 ± 2.09) μS/cm,(848.00 ± 84.53) min and(1.14 ± 0.02) m^(2)/g, respectively. The in vitro digestion experiment results showed that the TBNE remained stable in the stomach and was released in the intestine, while the size of TBNE in the gastrointestinal tact was significantly smaller than that of tributyrin(P < 0.05), which made it easier to be digested and absorbed. Compared with tributyrin treatment, TBNE significantly promoted the average body weight at the 7th day, average daily feed intake,average daily gain, feed/gain, ileum weight, and organ index of ileum of Hy-Line BROWN chicks(P < 0.05), and the butyric acid content in the ileal chyme from TBNE and tributyrin treatment were172.18 mg/m L and 100.85 mg/m L(P < 0.01). Therefore, the established TBNE technology in this study could be supposed to improve the digestion properties of tributyrin. | Feng Gao Yaoming Cui Shijia Song Jinfeng Zhu Xinyu Liu Xuyang Ji Hao Zhu Qingyu Jia Qiaohan Lin Junjun Guan | 2022 | Grain & Oil Science and Technology2022，5，3： | 0 |
| 6 | Theanine, a tea-plant-specific non-proteinogenic amino acid, is involved in the regulation of lateral root development in response to nitrogen status显示文摘Glutamine synthetase type I(GSI)-like proteins are proposed to mediate nitrogen signaling and developmental fate by synthesizing yet unidentified metabolites.Theanine,the most abundant non-proteinogenic amino acid in tea plants,is the first identified metabolite synthesized by a GSI-like protein(CsTSI)in a living system.However,the roles of theanine in nitrogen signaling and development are little understood.In this study we found that nitrogen deficiency significantly reduced theanine accumulation and increased lateral root development in tea plant seedlings.Exogenous theanine feeding significantly repressed lateral root development of seedlings of tea plants and the model plant Arabidopsis.The transcriptomic analysis revealed that the differentially expressed genes in the roots under theanine feeding were enriched in the apoplastic pathway and H2O_(2) metabolism.Consistently,theanine feeding reduced H2O_(2) levels in the roots.Importantly,when co-treated with H2O_(2),theanine abolished the promoting effect of H2O_(2) on lateral root development in both tea plant and Arabidopsis seedlings.The results of histochemical assays confirmed that theanine inhibited reactive oxygen species accumulation in the roots.Further transcriptomic analyses suggested the expression of genes encoding enzymes involved in H2O_(2) generation and scavenging was down-and upregulated by theanine,respectively.Moreover,the expression of genes involved in auxin metabolism and signaling,cell division,and cell expansion was also regulated by theanine.Collectively,these results suggested that CsTSI-synthesized theanine is likely involved in the regulation of lateral root development,via modulating H2O_(2) accumulation,in response to nitrogen levels in tea plants.This study also implied that the module consisting of GSI-like protein and theanine-like metabolite is probably conserved in regulating development in response to nitrogen status in plant species. | Tingting Chen Shijia Lin Ziping Chen Tianyuan Yang Shupei Zhang Jinsong Zhang Guohua Xu Xiaochun Wan Zhaoliang Zhang | 2023 | Horticulture Research2023，10，2： | 0 |
| 7 | Haem Oxygenase 1 is a potential target for creating etiolated/albino tea plants (Camellia sinensis) with high theanine accumulation显示文摘Theanine content is highly correlated with sensory quality and health benefits of tea infusion.The tender shoots of etiolated and albino tea plants contain higher theanine than the normal green tea plants and are valuable materials for high quality green tea processing.However,why these etiolated or albino tea plants can highly accumulate theanine is largely unknown.In this study,we observed an Arabidopsis etiolated mutant hy1–100(mutation in Haem Oxygenase 1,HO1)that accumulated higher levels of glutamine(an analog of theanine).We therefore identified CsHO1 in tea plants and found CsHO1 is conserved in amino acid sequences and subcellular localization with its homologs in other plants.Importantly,CsHO1 expression in the new shoots was much lower in an etiolated tea plants‘Huangkui’and an albino tea plant‘Huangshan Baicha’than that in normal green tea plants.The expression levels of CsHO1 were negatively correlated with theanine contents in these green,etiolated and albino shoots.Moreover,CsHO1 expression levels in various organs and different time points were also negatively correlated with theanine accumulation.The hy1–100 was hypersensitive to high levels of theanine and accumulated more theanine under theanine feeding,and these phenotypes were rescued by the expression of CsHO1 in this mutant.Transient knockdown CsHO1 expression in the new shoots of tea plant using antisense oligonucleotides(asODN)increased theanine accumulation.Collectively,these results demonstrated CsHO1 negatively regulates theanine accumulation in tea plants,and that low expression CsHO1 likely contributes to the theanine accumulation in etiolated/albino tea plants. | Ziping Chen Shijia Lin Tingting Chen Mengxue Han Tianyuan Yang Yan Wang Shilai Bao Zhougao Shen Xiaochun Wan Zhaoliang Zhang | 2023 | Horticulture Research2023，10，2： | 0 |
| 8 | CsGDH2.1 negatively regulates theanine accumulation in late-spring tea plants (Camellia sinensis var. sinensis)显示文摘Theanine,a unique and the most abundant non-proteinogenic amino acid in tea plants,endows tea infusion with the umami taste and anti-stress effects.Its content in tea correlates highly with green tea quality.Theanine content in new shoots of tea plants is high in mid-spring and greatly decreases in late spring.However,how the decrease is regulated is largely unknown.In a genetic screening,we observed that a yeast mutant,glutamate dehydrolase 2(gdh2),was hypersensitive to 40 mM theanine and accumulated more theanine.This result implied a role of CsGDH2s in theanine accumulation in tea plants.Therefore,we identified the two homologs of GDH2,CsGDH2.1 and CsGDH2.2,in tea plants.Yeast complementation assay showed that the expression of CsGDH2.1 in yeast gdh2 mutant rescued the theanine hypersensitivity and hyperaccumulation of this mutant.Subcellular localization and tissue-specific expression showed CsGDH2.1 localized in the mitochondria and highly expressed in young tissues.Importantly,CsGDH2.1 expression was low in early spring,and increased significantly in late spring,in the new shoots of tea plants.These results all support the idea that CsGDH2.1 regulates theanine accumulation in the new shoots.Moreover,the in vitro enzyme assay showed that CsGDH2.1 had glutamate catabolic activity,and knockdown of CsGDH2.1 expression increased glutamate and theanine accumulation in the new shoots of tea plants.These findings suggested that CsGDH2.1-mediated glutamate catabolism negatively regulates theanine accumulation in the new shoots in late spring,and provides a functional gene for improving late-spring green tea quality. | Tingting Chen Jingzhen Ma Huiping Li Shijia Lin Chunxia Dong Yunxia Xie Xiaomei Yan Shupei Zhang Tianyuan Yang Xiaochun Wan Zhaoliang Zhang | 2023 | Horticulture Research2023，10，1： | 0 |
| 9 | Numerical studies on the radiation uniformity of Z-pinch dynamic hohlraum显示文摘Radiation uniformity is important for Z-pinch dynamic hohlraum driven fusion. In order to understand the radiation uniformity of Z-pinchdynamic hohlraum, the code MULTI-2D with a new developed magnetic field package is employed to investigate the related physical processeson Julong-I facility with drive current about 7e8 MA. Numerical simulations suggest that Z-pinch dynamic hohlraum with radiation temperaturemore than 100 eV can be created on Julong-I facility. Although some X-rays can escape out of the hohlraum from Z-pinch plasma and electrodes, the radiation field near the foam center is quite uniform after a transition time. For the load parameters used in this paper, the transitiontime for the thermal wave transports from r = 1 mm to r = 0 mm is about 2.0 ns. Implosion of a testing pellet driven by cylindrical dynamichohlraum shows that symmetrical implosion is hard to achieve due to the relatively slow propagation speed of thermal wave and the compressionof cylindrical shock in the foam. With the help of quasi-spherical implosion, the hohlraum radiation uniformity and corresponding pelletimplosion symmetry can be significantly improved thanks to the shape modulation of thermal wave front and shock wave front. | Fuyuan Wu Yanyun Chu Rafael Ramis Zhenghong Li Yanyun Ma Jianlun Yang Zhen Wang Fan Ye Zhanchang Huang Jianmin Qi Lin Zhou Chuan Liang Shijia Chen Zheyi Ge Xiaohu Yang Shangwu Wang | 2018 | Matter and Radiation at Extremes2018，3，5： | 0 |
| 10 | Discovery of CECR2 Bromodomain Inhibitors with High Selectivities over BPTF Bromodomain显示文摘Cat's eye syndrome chromosome candidate 2 bromodomain(CECR2 BRD)and Bromodomain PHD transcription factor bromodomain(BPTF BRD)are the same subfamily proteins,both of which are highly conserved in sequence and binding pockets.Challenges remain in the development of small molecules to inhibit one of the two bromodomains(BRDs),in view of each subtype may possess unique physiological and pathological functions.There is still a lack of effective selective inhibitors of CECR2 BRD,which makes it difficult to fully understand the pathogenesis of CECR2-BRD in diseases,especially cancers.Herein,we report our efforts to discover a series of highly selective CECR2 BRD inhibitors over BPTF BRD based on TP-248.Structure-based molecular optimization led to the discovery of DC-CEi-26,whose IC_(50) for CECR2 BRD was 96.7±14.9 nmol/L and selectivity was up to 590×over BPTF BRD.DC-CEi-26 showed weak potencies for other classic BRDs in different subfamily,which may serve as a chemical probe for CECR2 BRD biological research. | Haibo Lu Shijia Zu Zhe Duan Yueyao Feng Jie Wang Jingyi Ma Qi Li Dongying Chen Bo Li Kaixian Chen Cheng Luo Jin Lin Tian Lu Hua Lin | 2022 | Chinese Journal of Chemistry2022，40，17： | 0 |
| 11 | Global multifaceted biodiversity patterns,centers,and conservation needs in angiosperms显示文摘The Convention on Biological Diversity seeks to conserve at least 30%of global land and water areas by 2030,which is a challenge but also an opportunity to better preserve biodiversity,including flowering plants(angiosperms).Herein,we compiled a large database on distributions of over 300,000 angiosperm species and the key functional traits of 67,024 species.Using this database,we constructed biodiversity-environment models to predict global patterns of taxonomic,phylogenetic,and functional diversity in terrestrial angiosperms and provide a comprehensive mapping of the three diversity facets.We further evaluated the current protection status of the biodiversity centers of these diversity facets.Our results showed that geographical patterns of the three facets of plant diversity exhibited substantial spatial mismatches and nonoverlapping conservation priorities.Idiosyncratic centers of functional diversity,particularly of herbaceous species,were primarily distributed in temperate regions and under weaker protection compared with other biodiversity centers of taxonomic and phylogenetic facets.Our global assessment of multifaceted biodiversity patterns and centers highlights the insufficiency and unbalanced conservation among the three diversity facets and the two growth forms(woody vs.herbaceous),thus providing directions for guiding the future conservation of global plant diversity. | Ao Luo Yaoqi Li Nawal Shrestha Xiaoting Xu Xiangyan Su Yichao Li Tong Lyu Kilara Waris Zhiyao Tang Xiaojuan Liu Luxiang Lin Yongsheng Chen Kuiling Zu Wenqi Song Shijia Peng Niklaus E.Zimmermann Loïc Pellissier Zhiheng Wang | 2024 | Science China(Life Sciences)2024，67，4： | 0 |
| 12 | Young Leaf White Stripe encodes a P-type PPR protein required for chloroplast development显示文摘Pentatricopeptide repeat(PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice(Oryza sativa), the detailed molecular functions of many PPR proteins remain unclear.Here, we characterized a rice young leaf white stripe(ylws) mutant, which has defective chloroplast development during early seedling growth.Map-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear-and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2,and rps12, and editing of ndhA, ndhB, and rps14transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development. | Jie Lan Qibing Lin Chunlei Zhou Xi Liu Rong Miao Tengfei Ma Yaping Chen Changling Mou Ruonan Jing Miao Feng Thanhliem Nguyen Yulong Ren Zhijun Cheng Xin Zhang Shijia Liu Ling Jiang Jianmin Wan | 2023 | Journal of Integrative Plant Biology2023，65，7： | 0 |
| 13 | The role of phosphatidylcholine 34:1 in the occurrence,development and treatment of ulcerative colitis显示文摘Lipid homeostasis is considered to be related to intestinal metabolic balance,while its role in the pathogenesis and treatment of ulcerative colitis(UC)remains largely unexplored.The present study aimed to identify the target lipids related to the occurrence,development and treatment of UC by comparing the lipidomics of UC patients,mice and colonic organoids with the corresponding healthy controls.Here,multi-dimensional lipidomics based on LC–QTOF/MS,LC–MS/MS and iMScope systems were constructed and used to decipher the alteration of lipidomic profiles.The results indicated that UC patients and mice were often accompanied by dysregulation of lipid homeostasis,in which triglycerides and phosphatidylcholines were significantly reduced.Notably,phosphatidylcholine 34:1(PC34:1)was characterized by high abundance and closely correlation with UC disease.Our results also revealed that down-regulation of PC synthase PCYT1 α and Pemt caused by UC modeling was the main factor leading to the reduction of PC34:1,and exogenous PC34:1 could greatly enhance the fumarate level via inhibiting the transformation of glutamate to N-acetylglutamate,thus exerting an anti-UC effect.Collectively,our study not only supplies common technologies and strategies for exploring lipid metabolism in mammals,but also provides opportunities for the discovery of therapeutic agents and biomarkers of UC. | Tengjie Yu Zhihao Zhou Shijia Liu Changjian Li Zhi-Wei Zhang Yong Zhang Wei Jin Keanqi Liu Shuying Mao Lei Zhu Lin Xie Guangji Wang Yan Liang | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员