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| 1 | High-Resolution Genome-wide Association Study Identifies Genomic Regions and Candidate Genes for Important Agronomic Traits in Wheat显示文摘Wheat(Triticum aestivum)is a major staple food crop worldwide.Genetic dissection of important agronomic traits is essential for continuous improvement of wheat yield to meet the demand of the world's growing population.We conducted a large-scale genome-wide association study(GWAS)using a panel of 768 wheat cultivars that were genotyped with 327609 single-nucleotide polymorphisms generated by genotyping-by-sequencing and detected 395 quantitative trait loci(QTLs)for 12 traits under 7 environments.Among them,273 QTLs were delimited to≤1.0-Mb intervals and 7 of them are either known genes(Rht-D,Vrn-B1,and Vrn-D1)that have been cloned or known QTLs(TaGA2ox8,APO1,TaSus1-7B,and Rht12)that were previously mapped.Eight putative candidate genes were identified for three QTLs that enhance spike seed setting and grain size using gene expression data and were validated in three bi-parental populations.Protein sequence analysis identified 33 putative wheat orthologs that have high identity with rice genes in QTLs affecting similar traits.Large r^2 values for additive effects observed among the QTLs for most traits indicated that the phenotypes of these identified QTLs were highly predictable.Results from this study demonstrated that significantly increasing GWAS population size and marker density greatly improves detection and identification of candidate genes underlying a QTL,solidifying the foundation for large-scale QTL fine mapping,candidate gene validation,and developing functional markers for genomics-based breeding in wheat. | Yunlong Pang Chunxia Liu Danfeng Wang Paul St.Amand Amy Bernardo Wenhui Li Fang He Linzhi Li Liming Wang Xiufang Yuan Lei Dong Yu Su Huirui Zhang Meng Zhao Yunlong Liangi Hongze Jia Xitong Shen Yue Lu Hongming Jiang Yuye Wu Anfei Li Honggang Wang Lingrang Kong Guihua Bai Shubing Liu | 2020 | Molecular Plant2020，13，9： | 8 |
| 2 | Comparison of nonhuman primates identified the suitable model for COVID-19显示文摘Identification of a suitable nonhuman primate(NHP)model of COVID-19 remains challenging.Here,we characterized severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in three NHP species:Old World monkeys Macaca mulatta(M.mulatta)and Macaca fascicularis(M.fascicularis)and New World monkey Callithrix jacchus(C.jacchus).Infected M.mulatta and M.fascicularis showed abnormal chest radiographs,an increased body temperature and a decreased body weight.Viral genomes were detected in swab and blood samples from all animals.Viral load was detected in the pulmonary tissues of M.mulatta and M.fascicularis but not C.jacchus.Furthermore,among the three animal species,M.mulatta showed the strongest response to SARS-CoV-2,including increased inflammatory cytokine expression and pathological changes in the pulmonary tissues.Collectively,these data revealed the different susceptibilities of Old World and New World monkeys to SARS-CoV-2 and identified M.mulatta as the most suitable for modeling COVID-19. | Shuaiyao Lu Yuan Zhao Wenhai Yu Yun Yang Jiahong Gao Junbin Wang Dexuan Kuang Mengli Yang Jing Yang Chunxia Ma Jingwen Xu Xingli Qian Haiyan Li Siwen Zhao Jingmei Li Haixuan Wang Haiting Long Jingxian Zhou Fangyu Luo Kaiyun Ding Daoju Wu Yong Zhang Yinliang Dong Yuqin Liu Yinqiu Zheng Xiaochen Lin Li Jiao Huanying Zheng Qing Dai Qiangming Sun Yunzhang Hu Changwen Ke Hongqi Liu Xiaozhong Peng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 3 | Single copper sites dispersed on hierarchically porous carbon for improving oxygen reduction reaction towards zinc-air battery显示文摘The demand for high-performance non-precious-metal electrocatalysts to replace the noble metal-based catalysts for oxygen reduction reaction(ORR)is intensively increasing.Herein,single-atomic copper sites supported on N-doped three-dimensional hierarchically porous carbon catalyst(Cu_(1)/NC)was prepared by coordination pyrolysis strategy.Remarkably,the Cu_(1)/NC-900 catalyst not only exhibits excellent ORR performance with a half-wave potential of 0.894 V(vs.RHE)in alkaline media,outperforming those of commercial Pt/C(0.851 V)and Cu nanoparticles anchored on N-doped porous carbon(CuNPs/NC-900),but also demonstrates high stability and methanol tolerance.Moreover,the Cu_(1)/NC-900 based Zn-air battery exhibits higher power density,rechargeability and cyclic stability than the one based on Pt/C.Both experimental and theoretical investigations demonstrated that the excellent performance of the as-obtained Cu_(1)/NC-900 could be attributed to the synergistic effect between copper coordinated by three N atoms active sites and the neighbouring carbon defect,resulting in elevated Cu d-band centers of Cu atoms and facilitating intermediate desorption for ORR process.This study may lead towards the development of highly efficient non-noble metal catalysts for applications in electrochemical energy conversion. | Wenjie Wu Yan Liu Dong Liu Wenxing Chen Zhaoyi Song Ximin Wang Yamin Zheng Ning Lu Chunxia Wang Junjie Mao Yadong Li | 2021 | Nano Research2021，14，4： | 4 |
| 4 | The tea plant CsLHT1 and CsLHT6 transporters take up amino acids, as a nitrogen source, from the soil of organic tea plantations显示文摘Organic tea is more popular than conventional tea that originates from fertilized plants.Amino acids inorganic soils constitute a substantial pool nitrogen(N)available for plants.However,the amino-acid contents in soils of tea plantations and how tea plants take up these amino acids remain largely unknown.In this study,we show that the amino-acid content in the soil of an organic tea plantation is significantly higher than that of a conventional tea plantation.Glutamate,alanine,valine,and leucine were the most abundant amino acids in the soil of this tea plantation.When 15 N-glutamate was fed to tea plants,it was efficiently absorbed and significantly increased the contents of other amino acids in the roots.We cloned seven CsLHT genes encoding amino-acid transporters and found that the expression of CsLHT1,CsLHT2,and CsLHT6 in the roots significantly increased upon glutamate feeding.Moreover,the expression of CsLHT1 or CsLHT6 in a yeast amino-acid uptake-defective mutant,22Δ10α,enabled growth on media with amino acids constituting the sole N source.Amino-acid uptake assays indicated that CsLHT1 and CsLHT6 are H^(+)-dependent high-and low-affinity amino-acid transporters,respectively.We further demonstrated that CsLHT1 and CsLHT6 are highly expressed in the roots and are localized to the plasma membrane.Moreover,overexpression of CsLHT1 and CsLHT6 in Arabidopsis significantly improved the uptake of exogenously supplied 15 N-glutamate and 15 N-glutamine.Taken together,our findings are consistent with the involvement of CsLHT1 and CsLHT6 in amino-acid uptake from the soil,which is particularly important for tea plants grown inorganic tea plantations. | Fang Li Chunxia Dong Tianyuan Yang Shilai Bao Wanping Fang William J.Lucas Zhaoliang Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 5 | Zein as a structural protein in gluten-free systems:an overview显示文摘Zein,a class of alcohol-soluble prolamines in maize endosperm,is mainly composed of α-zein,β-zein,andγ-zein.It has been recognized as a structural protein for various gluten-free systems since it can form glutenlike viscoelastic network.The formation of viscoelastic zein network can make up for the structural defect of gluten-free doughs caused by the lack of gluten.To make the most of structural functionality of zein in glutenfree foods,it is important to clearly elucidate the fundamental properties of zein network.In this article,these properties have been discussed,analyzed and summarized from the relationship between protein network and structural functionality of zein,the feature and formation mechanism of zein network,factors affecting zein network and the applications of zein network in improving the quality of gluten-free food.In addition,this article also looks forward to potential research areas on zein network. | Xinrui Zhang Chunxia Dong Yayun Hu Mengnan Gao Guangzhong Luan | 2021 | Food Science and Human Wellness2021，10，3： | 3 |
| 6 | Development and validation of an HPLC–MS/MS method to determine clopidogrel in human plasma显示文摘A quantitative method for clopidogrel using online-SPE tandem LC–MS/MS was developed and fully validated according to the well-established FDA guidelines.The method achieves adequate sensitivity for pharmacokinetic studies,with lower limit of quantifications(LLOQs)as low as 10 pg/m L.Chromatographic separations were performed on reversed phase columns Kromasil Eternity-2.5-C18-UHPLC for both methods.Positive electrospray ionization in multiple reaction monitoring(MRM)mode was employed for signal detection and a deuterated analogue(clopidogrel-d_4)was used as internal standard(IS).Adjustments in sample preparation,including introduction of an online-SPE system proved to be the most effective method to solve the analyte back-conversion in clinical samples.Pooled clinical samples(two levels)were prepared and successfully used as real-sample quality control(QC)in the validation of back-conversion testing under different conditions.The result showed that the real samples were stable in room temperature for 24 h.Linearity,precision,extraction recovery,matrix effect on spiked QC samples and stability tests on both spiked QCs and real sample QCs stored in different conditions met the acceptance criteria.This online-SPE method was successfully applied to a bioequivalence study of75 mg single dose clopidogrel tablets in 48 healthy male subjects. | Gangyi Liu Chunxia Dong Weiwei Shen Xiaopei Lu Mengqi Zhang Yuzhou Gui Qinyi Zhou Chen Yu | 2016 | Acta Pharmaceutica Sinica B2016，6，1： | 3 |
| 7 | Reduced graphene oxide supported Pd-Cu-Co trimetallic catalyst:synthesis,characterization and methanol electrooxidation properties显示文摘Trimetallic palladium-copper-cobalt nanoparticles supported on reduced graphene oxide(PdCuCo/RGO)with different molar ratios of Pd,Cu and Co can be synthesized by facile chemical reduction with NaBH_4 as reductant and cetrimonium bromide as stabilizer.The morphology,structure and composition of the as-synthesized catalysts are characterized by transmission electron microscopy,X-ray diffraction and Xray photoelectron spectroscopy.The cyclic voltammetry and chronoamperometry are utilized to investigate the electrochemical activities and stabilities of the as-obtained catalysts.The results demonstrate that the PdCuCo/RGO catalyst shows superior catalytic activity and stability for methanol electrooxidation in alkaline media compared with PdCu/RGO,PdCo/RGO,and Pd/RGO catalysts.These findings suggest that the PdCuCo/RGO catalyst possesses a great potential as a promising anode catalyst for direct methanol fuel cells. | Fan Yang Bing Zhang Sen Dong Chunxia Wang Andong Feng Xiaoxu Fan Yongfeng Li | 2019 | Journal of Energy Chemistry2019，28，2： | 2 |
| 8 | ApcD is required for state transition but not involved in blue-light induced quenching in the cyanobacterium Anabaena sp.PCC7120显示文摘Pbycobilisomes(PBS) are able to transfer absorbed energy to photosystem I and Ⅱ,and the distribution of light energy between two photosystems is regulated by state transitions.In this study we show that energy transfer from PBS to photosystem I(PSI) requires ApcD.Cells were unable to perform state transitions in the absence of ApcD.The apcD mutant grows more slowly in light mainly absorbed by PBS,indicating that ApcD-dependent energy transfer to PSI is required for optimal growth under this condition.The apcD mutant showed normal blue-light induced quenching,suggesting that ApcD is not required for this process and state transitions are independent of blue-light induced quenching.Under nitrogen fixing condition,the growth rates of the wild type and the mutant were the same,indicating that energy transfer from PBS to PSI in heterocysts was not required for nitrogen fixation. | DONG ChunXia ZHAO JinDong | 2008 | Chinese Science Bulletin2008，53，21： | 1 |
| 9 | Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice显示文摘Ulcerative colitis(UC)is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.Previous studies have indicated that celastrol(CSR)has strong anti-inflammatory and immune-inhibitory effects.Here,we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model,and addressed the mechanism by which CSR exerts the protective effects.We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining,intestinal permeability assay,cytokine assay,flow cytometry,fecal microbiota transplantation(FMT),16S rRNA sequencing,untargeted metabolomics,and cell differentiation.CSR administration significantly ameliorated the dextran sodium sulfate(DSS)-induced colitis in mice,which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index(DAI)score and intestinal permeability.Meanwhile,CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels,and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.Notably,all the therapeutic effects were exerted in a gut microbiota-dependent manner.Furthermore,CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites,which is probably associated with the gut microbiota-mediated protective effects.In conclusion,this study provides the strong evidence that CSR may be a promising therapeutic drug for UC. | Mingyue Li Weina Guo Yalan Dong Wenzhu Wang Chunxia Tian Zili Zhang Ting Yu Haifeng Zhou Yang Gui Kaming Xue Junyi Li Feng Jiang Alexey Sarapultsev Huafang Wang Ge Zhang Shanshan Luo Heng Fan Desheng Hu | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 1 |
| 10 | Studies on blended cement with a large amount of fly ash显示文摘 | Xinghua Fu Zhi Wang Wenhong Tao Chunxia Yang Wenping Hou Youjun Dong Xuequan Wu | 2002 | Cement and Concrete Research2002，，7： | 1 |
| 11 | Formation of β-Lactones by [2+2] Cycloaddition of Dichloroketene with Unreactive Carbonyl Compounds显示文摘 | DONG Xu ZHANG Chunxia XU Jian ZHANG Yue ZHAO Ying CHEN Li | 2018 | Chemical Research in Chinese Universities2018，34，5： | 0 |
| 12 | FMO3-TMAO axis modulates the clinical outcome in chronic heart-failure patients with reduced ejection fraction:evidence from an Asian population显示文摘The association among plasma trimethylamine-N-oxide(TMAO),FMO3 polymorphisms,and chronic heart failure(CHF)remains to be elucidated.TMAO is a microbiota-dependent metabolite from dietary choline and carnitine.A prospective study was performed including 955 consecutively diagnosed CHF patients with reduced ejection fraction,with the longest follow-up of 7 years.The concentrations of plasma TMAO and its precursors,namely,choline and carnitine,were determined by liquid chromatography-mass spectrometry,and the FMO3 E158K polymorphisms(rs2266782)were genotyped.The top tertile of plasma TMAO was associated with a significant increment in hazard ratio(HR)for the composite outcome of cardiovascular death or heart transplantation(HR=1.47,95%CI=1.13-1.91,P=0.004)compared with the lowest tertile.After adjustments of the potential confounders,higher TMAO could still be used to predict the risk of the primary endpoint(adjusted HR=1.33,95%CI=1.01-1.74,P=0.039).This result was also obtained after further adjustment for carnitine(adjusted HR=1.33,95%CI=1.01-1.74,P=0.039).The FM03 rs2266782 polymorphism was associated with the plasma TMAO concentrations in our cohort,and lower TMAO levels were found in the AA-genotype.Thus,higher plasma TMAO levels indicated increased risk of the composite outcome of cardiovascular death or heart transplantation independent of potential confounders,and the FMO3 AA-genotype in rs2266782 was related to lower plasma TMAO levels. | Haoran Wei Mingming Zhao Man Huang Chenze Li Jianing Gao Ting Yu Qi Zhang Xiaoqing Shen Liang Ji Li Ni Chunxia Zhao Zeneng Wang Erdan Dong Lemin Zheng Dao Wen Wang | 2022 | Frontiers of Medicine2022，16，2： | 0 |
| 13 | CsGDH2.1 negatively regulates theanine accumulation in late-spring tea plants (Camellia sinensis var. sinensis)显示文摘Theanine,a unique and the most abundant non-proteinogenic amino acid in tea plants,endows tea infusion with the umami taste and anti-stress effects.Its content in tea correlates highly with green tea quality.Theanine content in new shoots of tea plants is high in mid-spring and greatly decreases in late spring.However,how the decrease is regulated is largely unknown.In a genetic screening,we observed that a yeast mutant,glutamate dehydrolase 2(gdh2),was hypersensitive to 40 mM theanine and accumulated more theanine.This result implied a role of CsGDH2s in theanine accumulation in tea plants.Therefore,we identified the two homologs of GDH2,CsGDH2.1 and CsGDH2.2,in tea plants.Yeast complementation assay showed that the expression of CsGDH2.1 in yeast gdh2 mutant rescued the theanine hypersensitivity and hyperaccumulation of this mutant.Subcellular localization and tissue-specific expression showed CsGDH2.1 localized in the mitochondria and highly expressed in young tissues.Importantly,CsGDH2.1 expression was low in early spring,and increased significantly in late spring,in the new shoots of tea plants.These results all support the idea that CsGDH2.1 regulates theanine accumulation in the new shoots.Moreover,the in vitro enzyme assay showed that CsGDH2.1 had glutamate catabolic activity,and knockdown of CsGDH2.1 expression increased glutamate and theanine accumulation in the new shoots of tea plants.These findings suggested that CsGDH2.1-mediated glutamate catabolism negatively regulates theanine accumulation in the new shoots in late spring,and provides a functional gene for improving late-spring green tea quality. | Tingting Chen Jingzhen Ma Huiping Li Shijia Lin Chunxia Dong Yunxia Xie Xiaomei Yan Shupei Zhang Tianyuan Yang Xiaochun Wan Zhaoliang Zhang | 2023 | Horticulture Research2023，10，1： | 0 |
| 14 | A Hybrid Method for False Data Injection Attack Detection in Smart Grid Based on Variational Mode Decomposition and OS-ELM显示文摘Accurate state estimation is critical to wide-area situational awareness of smart grid.However,recent research found that power system state estimators are vulnerable to a new type of cyber-attack,called false data injection attack(FDIA).In order to ensure the security of power system operation and control,a hybrid FDIA detection mechanism utilizing temporal correlation is proposed.The proposed mechanism combines Variational Mode Decomposition(VMD)technology and machine learning.For the purpose of identifying the features of FDIA,VMD is used to decompose the system state time series into an ensemble of components with different frequencies.Furthermore,due to the lack of online model updating ability in a traditional extreme learning machine,an OS-extreme learning machine(OSELM)which has sequential learning ability is used as a detector for identifying FDIA.The proposed detection mechanism is evaluated on the IEEE-14 bus system using real load data from an independent system operator in New York.Apart from detection accuracy,the impact of attack intensity and environment noise on the performance of the proposed method are tested.The simulation results demonstrate the efficiency and robustness of our method. | Chunxia Dou Di Wu Dong Yue Bao Jin Shiyun Xu | 2022 | CSEE Journal of Power and Energy Systems2022，8，6： | 0 |
| 15 | Game and Dynamic Communication Path-Based Pricing Strategies for Microgrids Under Communication Interruption显示文摘Nowadays,the microgrid cluster is an important application scenario for energy trading.In trading,one of the most important research directions is the issue of pricing.To determine reasonable pricing for the microgrid cluster,data communication is used to create the cyber-physical system(CPS),which can improve the observability of microgrids.Then,the following works are carried out in the CPS.In the physical layer:1)Regarding trading between microgrids and the load,based on the generalized game theory,an optimal pricing strategy is proposed,which takes into account the interactive relationships among microgrids and transforms the pricing problem into a Nikaido-Isoda function to obtain the optimal prices conveniently;2)Regarding peer-to-peer trading between two microgrids,based on evolutionary game theory and the penalty mechanism,the optimal sale price of the seller is selected with boundary rationality.In the cyber layer,regarding the communication interruption issue existing in pricing(i.e.,the game process),based on the principle of matching the performance of the path with the importance degree of the data,a dynamic regulating method of paths is proposed,i.e.,adopting a new path to re-transmit the interrupted data to the destination.Finally,the effect of the proposed strategies is verified by case studies. | Bo Zhang Chunxia Dou Dong Yue Ju H.Park Yudi Zhang Zhanqiang Zhang | 2023 | IEEE/CAA Journal of Automatica Sinica2023，10，4： | 0 |
| 16 | PatU3 plays a central role in coordinating cell division and differentiation in pattern formation of filamentous cyanobacterium Nostoc sp.PCC 7120显示文摘Spatial periodic signal for cell differentiation in some multicellular organisms is generated according to Turing's principle for pattern formation.How a dividing cell responds to the signal of differentiation is addressed with the filamentous cyanobacterium Nostoc sp.PCC 7120,which forms the patterned distribution of heterocysts.We show that differentiation of a dividing cell was delayed until its division was completed and only one daughter cell became heterocyst.A mutant of patU3,which encodes an inhibitor of heterocyst formation,showed no such delay and formed heterocyst pairs from the daughter cells of cell division or dumbbell-shaped heterocysts from the cells undergoing cytokinesis.The patA mutant,which forms heterocysts only at the filament ends,restored intercalary heterocysts by a single nucleotide mutation of patU3,and double mutants of patU3/patA and patU3/hetF had the phenotypes of the patU3 mutant.We provide evidence that HetF,which can degrade PatU3,is recruited to cell divisome through its C-terminal domain.A HetF mutant with its N-terminal peptidase domain but lacking the C-terminal domain could not prevent the formation of heterocyst pairs,suggesting that the divisome recruitment of HetF is needed to sequester HetF for the delay of differentiation in dividing cells.Our study demonstrates that PatU3 plays a key role in celldivision coupled control of differentiation. | Lei Yin Zhenggao Zheng Yilin Li Xiying Li Dan Cheng Chunxia Dong Yixuan Liu Jindong Zhao | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 17 | High-performance fiber strain sensor of carbon nanotube/thermoplastic polyurethane@styrene butadiene styrene with a double percolated structure显示文摘In this work,a high-performance fiber strain sensor is fabricated by constructing a double percolated structure,consisting of carbon nanotube(CNT)/thermoplastic polyurethane(TPU)continuous phase and styrene butadiene styrene(SBS)phase,incompatible with TPU(CNT/TPU@SBS).Compared with other similar fiber strain sensor systems without double percolated structure,the CNT/TPU@SBS sensor achieves a lower percolation threshold(0.38 wt.%)and higher electrical conductivity.The conductivity of 1%-CNT/TPU@SBS(4.12×10^(-3) S·m^(-1))is two orders of magnitude higher than that of 1%-CNT/TPU(3.17×10^(-5) S·m^(-1))at the same CNT loading of 1 wt.%.Due to double percolated structure,the 1%-CNT/TPU@SBS sensor exhibits a wide strain detection range(0.2%-100%)and an ultra-high sensitivity(maximum gauge factor(GF)is 32411 at 100%strain).Besides,the 1%-CNT/TPU@SBS sensor shows a high linearity(R^(2)=0.97)at 0%-20%strain,relatively fast response time(214 ms),and stability(500 loading/unloading cycles).The designed sensor can efficiently monitor physiological signals and movements and identify load distribution after being woven into a sensor array,showing broad application prospects in wearable electronics. | Dong XIANG Libing LIU Xiaoyu CHEN Yuanpeng WU Menghan WANG Jie ZHANG Chunxia ZHAO Hui LI Zhenyu LI Ping WANG Yuntao LI | 2022 | Frontiers of Materials Science2022，16，1： | 0 |
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