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| 1 | Joint utilization of genetic analysis and semi-cloning technology reveals a digenic etiology of Müllerian anomalies显示文摘Dear Editor,Identifying pathogenic gene mutations and their combination is critical but challenging in dissecting the etiology of complex diseases when more than one gene is involved.1 The digenic/oligogenic/omnigenic models,holding that more than one gene could act synergistically,appeal to a wide range of genes responsible for complex phenotypes.1,2,3,4 These genetic models advanced our understanding of genetic factors underlying complex phenotypes,yet an accordingly rapid and efficient experimental assay for identifying pathogenic combinations of genetic variants at animal model level is lacking and urgently needed. | Lingbo Wang Ying Zhang Xiaoyi Fu Shuangshuang Dong Shuyan Tang Ning Zhang Chengcheng Song Nan Yang Lin Zhang Hongyan Wang Huijuan Shi Li Jin Feng Zhang Jinsong Li Keqin Hua | 2020 | Cell Research2020，30，1： | 8 |
| 2 | Influence of Nano-Al Concentrates on the Corrosion Resistance of Epoxy Coatings显示文摘A two-stage process was used to produce nano-composite epoxy coatings.The first step involved preparing nano-AI concentrates with high concentration and low viscosity,and the second step produced nanocomposite epoxy coatings by mixing the nano-AI concentrates and epoxy resin.Later,the coating was examined with immersion and salt spray tests.The coatings were characterized by electrochemical impedance spectroscopy(EIS),scanning electron microscopy(SEM) and X-ray photoelectron spectroscopy(XPS).The results showed that the 5%nano-AI significantly improves the corrosion resistance of the coatings.There are two effects of nano-AI on the coating.Nano-AI is corroded initially to protect the substrate from corrosion, and then the aluminum oxide and aluminum hydroxide were produced after corrosion of nano-AI,which hindered the transmission of corrosion fluid into the coatings. | Yongchun Liang Fu-Chun Liu Ming Nie Shuyan Zhao Jiedong Lin En-Hou Han | 2013 | Journal of Materials Science & Technology2013，29，4： | 4 |
| 3 | Anatomical characteristics of fusoid cells and vascular bundles in Fargesia yunnanensis leaves显示文摘As of today, the functions of fusoid cell, and the transport and loading pathways of photoassimilate in bamboo leaves are still not clear. In this paper, the leaves of Fargesia yunnanensis from a greenhouse and the wild were respectively used as samples to analyze the anatomical characteristics of fusoid cells and vascular bundles.The results showed that the bamboo leaves from greenhouse got shorter and thinner with fewer layers of palisade parenchyma cells than those from the wild. The volumes of fusoid cells were also increased. Fusoid cells originated from a huge parenchyma cell as testified by the observed nuclei. Several fusoid cells usually formed one cell complex close to the midrib. Crystals were detected in fusoid cells but no pits or plasmodesmata on their walls, suggesting that fusoid cells had the function of regulating water. The presence of fusoid cells determined the major difference between a leaf blade and sheath. There were prominent chloroplasts with simple stroma lamellae in the parenchymatous bundle sheath cells and starch grains were also observed in these chloroplast. Photoassimilates could be transported across vascular bundle sheath via symplasmic pathways for an abundant of plasmodesmata in sheath cell walls, and transported into phloem tube by apoplastic pathway as there were no pits in the walls of companion cells and phloem tubes. | Shuguang Wang Hui Zhang Shuyan Lin Chungyun Hse Yulong Ding | 2016 | Journal of Forestry Research2016，27，6： | 2 |
| 4 | Comparison of sustainability issues in two sensitive areas of China显示文摘 | Lin Zhen Shuyan Cao Yunjie Wei | 2009 | Environmental Science & Pol- icy2009，12，8： | 1 |
| 5 | Comprehensive identification and characterization of simple sequence repeats based on the whole-genome sequences of 14 forest and fruit trees显示文摘Simple sequence repeats(SSRs)are popular and important molecular markers that exist widely in plants.Here,we conducted a comprehensive identification and comparative analysis of SSRs in 14 tree species.A total of 16,298 SSRs were identified from 429,449 genes,and primers were successfully designed for 99.44% of the identified SSRs.Our analysis indicated that tri-nucleotide SSRs were the most abundant,with an average of~834 per species.Functional enrichment analysis by combining SSR-containing genes in all species,revealed 50 significantly enriched terms,with most belonging to transcription factor families associated with plant development and abiotic stresses such as Myeloblastosis_DNA-bind_4(Myb_DNA-bind_4),APETALA2(AP2),and Fantastic Four meristem regulator(FAF).Further functional enrichment analysis showed that 48 terms related to abiotic stress regulation and floral development were significantly enriched in ten species,whereas no significantly enriched terms were found in four species.Interestingly,the largest number of enriched terms was detected in Citrus sinensis(L.)Osbeck,accounting for 54.17% of all significantly enriched functional terms.Finally,we analyzed AP2 and trihelix gene families(Myb_DNA-bind_4)due to their significant enrichment in SSR-containing genes.The results indicated that whole-genome duplication(WGD)and whole genome triplication(WGT)might have played major roles in the expansion of the AP2 gene family but only slightly affected the expansion of the trihelix gene family during evolution.In conclusion,the identification and comprehensive characterization of SSR markers will greatly facilitate future comparative genomics and functional genomics studies. | Xiaoming Song Nan Li Yuanyuan Guo Yun Bai Tong Wu Tong Yu Shuyan Feng Yu Zhang Zhiyuan Wang Zhuo Liu Hao Lin | 2021 | Forestry Research2021，1，1： | 1 |
| 6 | Comparison of sustainability issues in two sensitive areas of China显示文摘 | Lin Zhen Shuyan Cao Yunjie Wei Oliver Dilly Xuelin Liu Fen Li Hannes Koenig Karen Tscherning Katharina Helming | 2009 | Environmental Science and Policy2009，，8： | 1 |
| 7 | Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense显示文摘This research reports on an efficient shoot proliferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense(Yi et R. S. Wang) Keng f.were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog(MS) medium containing 3.0 mg L-16-benzyladenine(BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L-1 BA, 0.5 mg L-1 kinetin(Kin), and 1.0 mg L-1 naphthaleneacetic acid(NAA). The highest frequency of callus formation(65.6%) was on MS medium containing 4.0 mg L-12,4-dichlorophenoxyacetic acid(2, 4-D), 0.5 mg L-1 NAA, and 0.1 mg L-1 thidiazuron(TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L-12,4-D, 0.5 mg L-1 TDZ and 0.5 mg L-1 NAA, and the optimum hormone combination was 4 mg L-1 BA ? 0.5 mg L-1 NAA for callus redifferentiation(up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L-1 NAA and 0.5 mg L-13-indole butyric acid(IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil ? perlite(1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication. | Shuyan Lin Guohua Liu Tingting Guo Li Zhang Shuguang Wang Yulong Ding | 2019 | Journal of Forestry Research2019，30，6： | 1 |
| 8 | Two Methods of Eval- uating Human Thermal Comfort Based on Non - uniform Envi- ronment 显示文摘 | Wang Baoguo Liu Shuyan Lin Huan | 2007 | International Journal of Man - Machine - Envi- ronment System Engineering2007，1，2： | 1 |
| 9 | Effect of NOx and SO2 on the photooxidation of methylglyoxal:Implications in secondary aerosol formation显示文摘Methylglyoxal(CH3COCHO,MG),which is one of the most abundant α-dicarbonyl compounds in the atmosphere,has been reported as a major source of secondary organic aerosol(SOA).In this work,the reaction of MG with hydroxyl radicals was studied in a 500 L smog chamber at(293±3)K,atmospheric pressure,(18±2)%relative humidity,and under different NOx and SO2.Particle size distribution was measured by using a scanning mobility particle sizer(SMPS)and the results showed that the addition of SO2 can promote SOA formation,while different NOx concentrations have different influences on SOA production.High NOx suppressed the SOA formation,whereas the particle mass concentration,particle number concentration and particle geometric mean diameter increased with the increasing NOx concentration at low NOx concentration in the presence of SO2.In addition,the products of the OH-initiated oxidation of MG and the functional groups of the particle phase in the MG/OH/SO2 and MG/OH/NOx/SO2 reaction systems were detected by gas chromatography mass spectrometry(GC-MS)and attenuated total reflection fourier transformed infrared spectroscopy(ATR-FTIR)analysis.Two products,glyoxylic acid and oxalic acid,were detected by GC-MS.The mechanism of the reaction of MG and OH radicals that follows two main pathways,H atom abstraction and hydration,is proposed.Evidence is provided for the formation of organic nitrates and organic sulfate in particle phase from IR spectra.Incorporation of NOx and SO2 influence suggested that SOA formation from anthropogenic hydrocarbons may be more efficient in polluted environment. | Shuyan Wang Lin Du Narcisse T.Tsona Xiaotong Jiang Bo You Li Xu Zhaomin Yang Wenxing Wang | 2020 | Journal of Environmental Sciences2020，32，6： | 1 |
| 10 | Arable land requirements based on food consumption patterns: Case study in rural Guyuan District, Western China显示文摘 | Lin Zhen Shuyan Cao Shengkui Cheng Gaodi Xie Yunjie Wei Xuelin Liu Fen Li | | Ecological Economics0，，： | 1 |
| 11 | Technologies for Asphalt Pavement Surface Testing in Road and Bridge Construction显示文摘Asphalt pavement is currently one of the main components in the construction of roads and bridges.However,from a practical point of view,various quality problems are prone to occur in the surface layer of asphalt pavement,which will lead to the poor overall quality of road and bridge projects.Therefore,it should be applied reasonably.Advanced testing technologies are used to test the mixture quality,compaction,segregation,thickness,and other aspects of the asphalt pavement surface layer,so as to improve the quality of the asphalt pavement surface layer,and then improve the overall quality of road and bridge construction.Therefore,this paper mainly analyzes the technologies for asphalt pavement surface layer testing in road and bridge engineering construction. | Lu Zhang Guangjun Li Lin Nan Shuyan Liu | 2023 | Journal of Architectural Research and Development2023，7，2： | 0 |
| 12 | Analysis of New Materials Application in Municipal Road Construction显示文摘In order to promote the development of road traffic in our country,it is necessary to strengthen the research on municipal road construction technology,constantly innovate construction technology and construction technique,and then effectively ensure the rapid development of urban traffic.This paper mainly elaborates on asphalt road surface regeneration technology,modified asphalt concrete,concrete road surface anti-cracking technology,three-dimensional printing technology,composite material road surface,polymer composite materials,etc.,to ensure the development of urban transportation and the quality of municipal road projects. | Guangjun Li Lu Zhang Lin Nan Manman Feng Shuyan Liu | 2023 | Journal of Architectural Research and Development2023，7，3： | 0 |
| 13 | Comparative analysis of long noncoding RNAs in angiosperms and characterization of long noncoding RNAs in response to heat stress in Chinese cabbage显示文摘Long noncoding RNAs(lncRNAs)are widely present in different species and play critical roles in response to abiotic stresses.However,the functions of lncRNAs in Chinese cabbage under heat stress remain unknown.Here,we first conducted a global comparative analysis of 247,242 lncRNAs among 37 species.The results indicated that lncRNAs were poorly conserved among different species,and only 960 lncRNAs were homologous to 524 miRNA precursors.We then carried out lncRNA sequencing for a genome-wide analysis of lncRNAs and their target genes in Chinese cabbage at different stages of heat treatment.In total,18,253 lncRNAs were identified,of which 1229 differentially expressed(DE)lncRNAs were characterized as being heat-responsive.The ceRNA network revealed that 38 lncRNAs,16 miRNAs,and 167 mRNAs were involved in the heat response in Chinese cabbage.Combined analysis of the cis-and trans-regulated genes indicated that the targets of DE lncRNAs were significantly enriched in the“protein processing in endoplasmic reticulum”and“plant hormone signal transduction”pathways.Furthermore,the majority of HSP and PYL genes involved in these two pathways exhibited similar expression patterns and responded to heat stress rapidly.Based on the networks of DE lncRNA-mRNAs,29 and 22 lncRNAs were found to interact with HSP and PYL genes,respectively.Finally,the expression of several critical lncRNAs and their targets was verified by qRT-PCR.Overall,we conducted a comparative analysis of lncRNAs among 37 species and performed a comprehensive analysis of lncRNAs in Chinese cabbage.Our findings expand the knowledge of lncRNAs involved in the heat stress response in Chinese cabbage,and the identified lncRNAs provide an abundance of resources for future comparative and functional studies. | Xiaoming Song Jingjing Hu Tong Wu Qihang Yang Xuehuan Feng Hao Lin Shuyan Feng Chunlin Cui Ying Yu Rong Zhou Ke Gong Tong Yu Qiaoying Pei Nan Li | 2021 | Horticulture Research2021，8，1： | 0 |
| 14 | The structures of floral organs and reproductive characteristics of an ornamental bamboo species,Pleioblastus pygmaeus显示文摘As a woody grass,bamboo has special reproductive habits with an extremely long vegetative phase.Pleioblastus pygmaeus is a kind of ornamental dwarf bamboo species with high ecological and economic value in China.There was no documentary record of the flowering of P.pygmaeus until 2015,which bloomed in the Bamboo Garden of Nanjing Forestry University.Generally,bamboo plants rarely bear fruit or have a low seed setting rate,but this species has a high seed production.This study explores developmental reasons using anatomical methods.The process of flower bud differentiation and a series of important reproductive development processes including anther wall development,microsporogenesis,megasporogenesis,male and female gametophyte development,and embryonic development were investigated sequentially.Each stamen contained three tetrasporangiate anthers and the development of anther wall was of Monocotyledonous type.The microspores finally matured into 3-celled pollen grains by two successive mitoses.The pistil was composed of three carpels,with an anatropous,tenuinucellate and bitegmic ovule in a single ovary.The formation of embryo sac belonged to Polygonum type.With three mitoses,functional megaspore developed into the embryo sac with eight nuclei within seven cells.The development of stamen and pistil was synchronous in the same floret.The development of embryo followed Grass type and the development of endosperm belonged to Nuclear type.The development of endosperm was earlier than that of embryo.The study enriches basic knowledge of reproductive biology of bamboo plants and is of great fundamental significance to furtherly explore flowering mechanism of P.pygmaeus and to cultivate bamboo plants sustainably. | Wenjing Yao Chuanzhe Li Shuyan Lin Jianping Wang Tingting Fan Wanqi Zhao | 2023 | Horticultural Plant Journal2023，9，3： | 0 |
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