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| 1 | Diverse modes of clonal evolution in HBV-related hepatocellular carcinoma revealed by single-cell genome sequencing显示文摘Hepatocellular 癌(HCC ) 是癌症实质词法,基因并且 phenotypic 差异。然而,我们不理解在 intratumor 异质和肿瘤的联系词法 / 组织学的特征之间的关系。用介绍 96 个肿瘤房间(30-36 各个) 和 15 个正常的肝房间(5 各个) 的单个房间的整个染色体的定序,与联系 HBV 的 HCC 从三个男病人收集了,我们证实拷贝数字变化在 hepatocarcinogenesis 早发生,但是此后在整个肿瘤仍然保持相对稳定前进。重要地,我们证明特定的 HCC 能具有 monoclonal 或 polyclonal 起源。有汇合的 multinodular 形态学的肿瘤是典型 polyclonal 肿瘤并且显示最高的 intratumor 异质。除了 mutational 和拷贝数字侧面,我们把了用导出 HBV 的外国 genomic 标记的 HCC 的同种细胞的起源。在 monoclonal HCC,所有肿瘤单身者房间展出一样的 HBV 集成,显示 HBV 集成是一个早司机事件并且在肿瘤前进期间仍然保持极其稳定。另外,我们的结果显示那两个都转移,迟了的传播并且早播种当模特儿,在 HCC 有一个角色前进。尤其是,开始的克隆传播的早 intrahepatic 导致同步 multifocal 肿瘤的形成。同时,我们在 HCC 识别了潜在的司机基因 ZNF717,它展出变化的高频率在单个房间并且人口铺平,,通过调整 IL-6/STAT3 小径行动的肿瘤 suppressor。这些调查结果热点多重不同肿瘤在 HCC 的进化机制,它为特定的处理策略建议需要。 | Meng Duan, Shu Zhang, Zhichao Wang, Jieyi Shi, Longzi Liu, Xiaoying Wang, Aiwu Ke, Jian Zhou, Jia Fan, Qiang Gao Junfeng Hao, Chong Li Sijia Cui Daniel L Worthley Ya Cao Ruibin Xi Xiaoming Zhang Jian Zhou, Jia Fan Qiang Gao | 2018 | Cell Research2018，28，3： | 21 |
| 2 | Kinetic hydrate inhibitor performance of new copolymer poly(N-vinyl-2-pyrrolidone-co-2-vinyl pyridine)s with TBAB显示文摘In oil and gas field, the application of kinetic hydrate inhibitors (KHIs) independently has remained problematic in high subcooling and high water-cut situation. One feasible method to resolve this problem is the combined use of KHIs and some synergists, which would enhance KHIs’ inhibitory effect on both hydrate nucleation and hydrate crystal growth. In this study, a novel kind of KHI copolymer poly(N-vinyl-2-pyrrolidone-co-2-vinyl pyridine)s (HGs) is used in conjunction with TBAB to show its high performance on hydrate inhibition. The performance of HGs with different monomer ratios in structure II tetrahydrofuran (THF) hydrate is investigated using kinetic hydrate inhibitor evaluation apparatus by step-cooling method and isothermal cooling method. With the combined gas hydrate inhibitor at the concentration of 1.0 wt%, the induction time of 19 wt% THF solution could be prolonged to 8.5 h at a high subcooling of 6℃. Finally, the mechanism of HGs inhibiting the formation of gas hydrate is proposed. | Jun Hu Sijia Li Yanhong Wang Xuemei Lang Qingping Li Shuanshi Fan | 2012 | Journal of Natural Gas Chemistry2012，21，2： | 8 |
| 3 | Brain-wide Mapping of Mono-synaptic Afferents to Different Cell Types in the Laterodorsal Tegmentum显示文摘The laterodorsal tegmentum(LDT) is a brain structure involved in distinct behaviors including arousal,reward, and innate fear. How environmental stimuli and top-down control from high-order sensory and limbic cortical areas converge and coordinate in this region to modulate diverse behavioral outputs remains unclear.Using a modified rabies virus, we applied monosynaptic retrograde tracing to the whole brain to examine the LDT cell type specific upstream nuclei. The LDT received very strong midbrain and hindbrain afferents and moderate cortical and hypothalamic innervation but weak connections to the thalamus. The main projection neurons from cortical areas were restricted to the limbic lobe, including the ventral orbital cortex(VO), prelimbic, and cingulate cortices. Although different cell populations received qualitatively similar inputs, primarily via afferents from the periaqueductal gray area, superior colliculus, and the LDT itself, parvalbumin-positive(PV?) GABAergic cells received preferential projections from local LDT neurons.With regard to the different subtypes of GABAergic cells, aconsiderable number of nuclei, including those of the ventral tegmental area, central amygdaloid nucleus, and VO, made significantly greater inputs to somatostatinpositive cells than to PV?cells. Diverse inputs to the LDT on a system-wide level were revealed. | Xiaomeng Wang Hongbin Yang Libiao Pan Sijia Hao Xiaotong Wu Li Zhan Yijun Liu Fan Meng Huifang Lou Ying Shen Shumin Duan Hao Wang | 2019 | Neuroscience Bulletin2019，35，5： | 3 |
| 4 | Multifunctional microcapsules:A theranostic agent for US/MR/PAT multi-modality imaging and synergistic chemo-photothermal osteosarcoma therapy显示文摘Development of versatile theranostic agents that simultaneously integrate therapeutic and diagnostic features remains a clinical urgent.Herein,we aimed to prepare uniform PEGylated(lactic-co-glycolic acid)(PLGA)microcapsules(PB@(Fe_(3)O_(4)@PEG-PLGA)MCs)with superparamagnetic Fe3O4 nanoparticles embedded in the shell and Prussian blue(PB)NPs inbuilt in the cavity via a premix membrane emulsification(PME)method.On account of the eligible geometry and multiple load capacity,these MCs could be used as efficient multi-modality contrast agents to simultaneously enhance the contrasts of US,MR and PAT imaging.In-built PB NPs furnished the MCs with excellent photothermal conversion property and embedded Fe_(3)O_(4)NPs endowed the magnetic location for fabrication of targeted drug delivery system.Notably,after further in-situ encapsulation of antitumor drug of DOX,(PB+DOX)@(Fe_(3)O_(4)@PEG-PLGA)MCs possessed more unique advantages on achieving near infrared(NIR)-responsive drug delivery and magnetic-guided chemo-photothermal synergistic osteosarcoma therapy.In vitro and in vivo studies revealed these biocompatible(PB+DOX)@(Fe_(3)O_(4)@PEG-PLGA)MCs could effectively target to the tumor tissue with superior therapeutic effect against the invasion of osteosarcoma and alleviation of osteolytic lesions,which will be developed as a smart platform integrating multi-modality imaging capabilities and synergistic effect with high therapy efficacy. | Hufei Wang Sijia Xu Daoyang Fan Xiaowen Geng Guang Zhi Decheng Wu Hong Shen Fei Yang Xiao Zhou Xing Wang | 2022 | Bioactive Materials2022，7，1： | 2 |
| 5 | Palmprint Phenotype Feature Extraction and Classification Based on Deep Learning显示文摘Palmprints are of long practical and cultural interest.Palmprint principal lines,also called primary palmar lines,are one of the most dominant palmprint features and do not change over the lifespan.The existing methods utilize filters and edge detection operators to get the principal lines from the palm region of interest(ROI),but can not distinguish the principal lines from fine wrinkles.This paper proposes a novel deep-learning architecture to extract palmprint principal lines,which could greatly reduce the influence of fine wrinkles,and classify palmprint phenotypes further from 2D palmprint images.This architecture includes three modules,ROI extraction module(REM)using pre-trained hand key point location model,principal line extraction module(PLEM)using deep edge detection model,and phenotype classifier(PC)based on ResNet34 network.Compared with the current ROI extraction method,our extraction is competitive with a success rate of 95.2%.For principal line extraction,the similarity score between our extracted lines and ground truth palmprint lines achieves 0.813.And the proposed architecture achieves a phenotype classification accuracy of 95.7%based on our self-built palmprint dataset CAS_Palm. | Fan Jinxi Li Shaoying Song Haiguo Zhang Sijia Wang Guangtao Zhai | 2022 | Phenomics2022，2，4： | 1 |
| 6 | Metal-organic frameworks-derived hierarchical ZnO structures as efficient sensing materials for formaldehyde detection显示文摘Semiconducting metal oxides have been considered as effective approach for designing highperformance chemical sensing materials.In this paper,a kind of metal-organic frameworks ZIF-8 was used as sacrificed template to prepare porous ZnO hollow nanocubes for the application in gas sensing.It is found that changing calcination temperature and solvent can greatly influence the morphology of the material,which finally affects the gas sensing performance.Acetylene-sensing properties of the sensors were investigated in detail.It can be clearly seen that the material used methanol as reaction solvent with the decomposition at 350℃for 2 h(ZnO-350-M)showed the optimal formaldehyde-sensing behaviors compared with other materials prepared in this experiment.The dynamic transients of the ZnO-350-M-based sensors demonstrated a high response value(about 10),fast response and recovery rate(4 s and 4 s,respectively)and good selectivity towards 100 ppm(part per million)formaldehyde as well as a low detectable limit(1 ppm).As exemplified for the sensing inve stigation towards formaldehyde,the porous ZnO hollow nanocubes showed a significantly improved chemical sensitivity due to the highly syne rgistic effects from the well exposed surfaces,defect states and the robust ZnO. | Nan Zhang Luming Yan Yang Lu Yizhuo Fan Sijia Guo Samira Adimi Dali Liu Shengping Ruan | 2020 | Chinese Chemical Letters2020，31，8： | 1 |
| 7 | Effect of anionic PAMAM with amido groups starburst dendrimers on the crystallization of Ca10(PO4)6(OH)2 by hydrothermal method 显示文摘 | YAN Sijia ZHOU Zhuohua ZHANG Fan | 2006 | Mater Chem Phys2006，99，1： | 1 |
| 8 | The Integrative Studies on the Functional A-to-I RNA Editing Events in Human Cancers显示文摘Adenosine-to-inosine(A-to-I)RNA editing,constituting nearly 90%of all RNA editing events in humans,has been reported to contribute to the tumorigenesis in diverse cancers.However,the comprehensive map for functional A-to-I RNA editing events in cancers is still insufficient.To fill this gap,we systematically and intensively analyzed multiple tumorigenic mechanisms of A-to-I RNA editing events in samples across 33 cancer types from The Cancer Genome Atlas.For individual candidate among1,500,000 quantified RNA editing events,we performed diverse types of downstream functional annotations.Finally,we identified 24,236 potentially functional A-to-I RNA editing events,including the cases in APOL1,IGFBP3,GRIA2,BLCAP,and miR-589-3p.These events might play crucial roles in the scenarios of tumorigenesis,due to their tumor-related editing frequencies or probable effects on altered expression profiles,protein functions,splicing patterns,and microRNA regulations of tumor genes.Our functional A-to-I RNA editing events(http://gffzz53f2f6c9ddf14a6bsbwx0wcnnfok966n0.ffgz.tsg.suse.edu.cn/CAeditome/)will help better understand the cancer pathology from the A-to-I RNA editing aspect. | Sijia Wu Zhiwei Fan Pora Kim Liyu Huang Xiaobo Zhou | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 9 | Jieduquyuziyin Prescription Suppresses the Inflammatory Activity of Macrophages via NOTCH1/NF-κB Pathway显示文摘Background:Jieduquyuziyin prescription(JP)is a traditional Chinese medicine(TCM)formula,which has been applied to the treatment of systemic lupus erythematosus(SLE)for decades,and its efficacy and safety have been confirmed in clinical practice.However,little is known about its molecular mechanism.Objective:To explore the effects of JP on macrophages’inflammatory activity and NOTCH1/NF-κB pathway.Methods:The JP-treated serum was prepared to determine its optimal concentration.Given the fact that active components in rats’serum might affect the results,control serum without JP components was prepared simul-taneously.Lipopolysaccharide(LPS)was used to activate RAW264.7,and the cells were interfered with DAPT(NOTCH1 blocker),control serum,and JP-treated serum,respectively.After the above intervention,the expres-sion of NOTCH1 and RBPJ,the nuclear translocation of NF-κB,and the extracellular release of IL6,TNF��,and NO,was evaluated by real-time reverse transcription-polymerase chain reaction(RT-PCR),western blotting(WB),enzyme-linked immunosorbent assay(ELISA),and Griess method.Results:Both DAPT and JP-treated serum could significantly suppress the expression of NOTCH1 and RBPJ induced by LPS,as well as the nuclear translocation of NF-κB,leading to the decreased release of IL6,TNF��,and NO,while control serum had little effect on macrophage activity and NOTCH1/NF-κB pathway.Conclusion:These results demonstrated the effects of JP on macrophage activation and pro-inflammatory response and suggested that the molecular mechanism of JP might attribute to the inhibition of the NOTCH1/NF-κB path-way.Besides,previous studies suggested that paeoniflorin and ferulic acid are two major effective components in JP.In subsequent experiments,we would further explore the effects of these two components on MRL/lpr mice and macrophage activity. | Sijia Fang Lina Ji Shan Wu Xiaoxuan Yang Kepeng Yang Yongsheng Fan | 2022 | Clinical Complementary Medicine and Pharmacology2022，2，3： | 0 |
| 10 | Efficacy of Baishao Luoshi decoction(白芍络石方)on synaptic plasticity in rats with post stroke spasticity显示文摘OBJECTIVE:To evaluate the efficacy of Baishao Luoshi decoction(白芍络石方,BD)on synaptic plasticity in rats with post stroke spasticity(PSS),and to study the mechanism behind the action.METHODS:The PSS model of rat was established by middle cerebral artery occlusion(MCAO).The neurological deficit symptoms were evaluated by modified neurological deficit score(mN SS).Muscle tension were evaluated by Modified Ashworth score(MAS).Transmission electron microscopy(TEM)was used to observe the synaptic ultrastructure.The expression of synaptic plasticity-related protein brain derived neurotrophic factor(BDNF),growth associated protein-43(GAP43),synaptophysin(p38)and microtubule-associated protein 2(MAP2)in the brain tissue around the infarct were detected by Western blotting.RESULTS:We found that mNSS were significantly improved and limb spasticity was ameliorated treated by BD.The thickness of postsynaptic density and the synaptic curvature increased significantly.The expression of synaptic plasticity-related protein BDNF,GAP43,p38,MAP2 in the brain tissue around the infarct were raised remarkably after treated by BD.CONCLUSIONS:Alleviating PSS by BD may be related to rescuing the synaptic plasticity,which provides a probable new therapeutic method for PSS. | XIE Le MAO Guo XIE Yao CAO Sijia ZHOU Shen JIANG Junlin YAO Ting FAN Jianhu LIU Dong KANG Fuliang WU Dahua GE Jinwen | 2023 | Journal of Traditional Chinese Medicine2023，43，2： | 0 |
| 11 | Genetic evidence for facial variation being a composite phenotype of cranial variation and facial soft tissue thickness显示文摘Facial and cranial variation represent a multidimensional set of highly correlated and heritable phenotypes.Little is known about the genetic basis explaining this correlation.We develop a software package ALo SFL for simultaneous localization of facial and cranial landmarks from head computed tomography(CT)images,apply it in the analysis of head CT images of 777 Han Chinese women,and obtain a set of phenotypes representing variation in face,skull and facial soft tissue thickness(FSTT).Association analysis of 301 single nucleotide polymorphisms(SNPs)from 191 distinct genomic loci previously associated with facial variation reveals an unexpected larger number of loci showing significant associations(P<1e-3)with cranial phenotypes than expected under the null(O/E=3.39),suggesting facial and cranial phenotypes share a substantial proportion of genetic components.Adding FSTT to a SNP-only model shows a large impact in explaining facial variance.A gene ontology analysis reveals that bone morphogenesis and osteoblast differentiation likely underlie our cranial-significant findings.Overall,this study simultaneously investigates the genetic effects on both facial and cranial variation of the same sample,supporting that facial variation is a composite phenotype of cranial variation and FSTT. | Wei Qian Manfei Zhang Kaiwen Wan Yunxia Xie Siyuan Du Jiarui Li Xiongzheng Mu Jiange Qiu Xiangyang Xue Xiahai Zhuang Yingzhi Wu Fan Liu Sijia Wang | 2022 | Journal of Genetics and Genomics2022，49，10： | 0 |
| 12 | Human umbilical cord blood mesenchymal stem cells conditioned media inhibits hypoxia-induced apoptosis in H9c2 cells by activation of the survival protein Akt显示文摘This work aimed to study the beneficial role of human umbilical cord blood-derived mesenchymal stem cellconditioned medium(MSC-CM)in hypoxia-induced apoptosis in H9c2 cardiomyoblasts,in which the serine/heroine kinases(Akt)pathway would be involved.For this,CM was collected by culturing MSCs in serum-free DMEM medium for 24 h,and paracrine factors were analyzed by protein chip.H9c2 cells were divided into the following groups:control group,hypoxia group,MSC-CM intervention group(CM group),MSC-CM+Akt phosphorylation inhibitor(LY294002)group(LY group).Apoptosis of the H9c2 cells was tested with chromatin dye Hoechst 33342 and FITC-conjugated Annexin V apoptosis detection kit by flow cytometer after a hypoxia/serum deprivation(H/SD)for 24 h.The apoptosis-related proteins were evaluated by Western blot.MSC-CM displayed significantly elevated levels of growth factors,anti-inflammatory,and anti-apoptosis cytokines.On Hoechst 33342 apoptosis staining,the H9c2 cell morphology displayed a lower proportion of apoptosis in the CM group than those in the hypoxia group,while apoptosis was increased in LY group.Flow cytometer analysis revealed the apoptosis ratio in the CM group was lower than the hypoxia group(12.34±2.00%vs.21.73±2.58%,p<0.05),while the LY group was significantly higher(22.54±3.89%).Active caspase-3 expression was increased in hypoxia group than control group(p<0.05),but decreased in CM group(p<0.01).Umbilical cord blood-derived mesenchymal stem cell-conditioned media secrete multiple paracrine factors that are able to inhibit hypoxia-induced H9c2 cardiomyoblasts apoptosis,and in which the activation of Akt phosphorylation is involved to achieve the protective effect. | XUHE GONG GUANGPU FAN HUI LIU SIJIA WANG RONGCHONG HUANG | 2020 | BIOCELL2020，44，3： | 0 |
| 13 | Protective mechanism of Paeoniae Radix Alba against chemical liver injury based on network pharmacology,molecular docking,and in vitro experiments显示文摘Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs. | Shuangqiao Liu Xin Liu Sijia Jiang Min Fu Jinxi Hu Jiaqi Liu Xiaoxu Fan Yingtong Feng Shujing Zhang Jingxia Wang | 2024 | Journal of Traditional Chinese Medical Sciences2024，11，1： | 0 |
| 14 | 神经细胞内新型谷氨酸生物合成通路参与日光照射改善学习记忆显示文摘文章简介日光照射可以影响情绪、学习以及认知等神经系统相关行为。然而,其分子和细胞水平的机制仍然不清楚。本研究揭示了适度的紫外线照射可提高血液中的尿刊酸水平,随后尿刊酸会透过血脑屏障进入大脑。接下来,研究人员用自主研发的单细胞质谱技术并与同位素示踪技术相结合. | 朱洪影 王宁 Lei Yao Qi Chen Ran Zhang Junchao Qian Yiwen Hou Weiwei Guo Sijia Fan Siling Liu Qiaoyun Zhao Feng Du Xin Zuo Yujun Guo Yan Xu Jiali Li Tian Xue Kai Zhong Xiaoyuan Song 黄光明 熊伟 | 2019 | 科学新闻2019，0，2： | 0 |
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