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| 1 | Bone marrow-derived mesenchymal stem cells migrate to healthy and damaged salivary glands following stem cell infusion显示文摘Xerostomia is a severe side effect of radiation therapy in head and neck cancer patients. To date, no satisfactory treatment option has been established. Because mesenchymal stem cells(MSCs) have been identified as a potential treatment modality, we aimed to evaluate stem cell distribution following intravenous and intraglandular injections using a surgical model of salivary gland damage and to analyse the effects of MSC injections on the recruitment of immune cells. The submandibular gland ducts of rats were surgically ligated. Syngeneic adult MSCs were isolated, immortalised by simian virus 40(SV40) large T antigen and characterized by flow cytometry. MSCs were injected intravenously and intraglandularly. After 1, 3 and 7 days, the organs of interest were analysed for stem cell recruitment. Inflammation was analysed by immunohistochemical staining. We were able to demonstrate that, after intravenous injection, MSCs were recruited to normal and damaged submandibular glands on days 1, 3 and 7. Unexpectedly, stem cells were recruited to ligated and non-ligated glands in a comparable manner. After intraglandular injection of MSCs into ligated glands, the presence of MSCs, leucocytes and macrophages was enhanced, compared to intravenous injection of stem cells. Our data suggest that injected MSCs were retained within the inflamed glands, could become activated and subsequently recruited leucocytes to the sites of tissue damage. | Silke Schwarz Ralf Huss Michaela Schulz-Siegmund Breda Vogel Sven Brandau Stephan Lang Nicole Rotter | 2014 | International Journal of Oral Science2014，6，3： | 5 |
| 2 | Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype. | C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf | 2014 | World Journal of Cardiology2014，6，4： | 3 |
| 3 | Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression显示文摘 | Magis Mandapathil Stephan Lang Elieser Gorelik Theresa L. Whiteside | 2009 | Journal of Immunological Methods2009，，1： | 1 |
| 4 | Elevated Serum Calcium and Parathormone levels in HIV-afflicted female heroine addicts显示文摘 | TEICHMANN J STEPHAN E LANGE V | 1997 | Eur J Med Res1997，2，： | 1 |
| 5 | Simultaneous quantitative analysis of ternary mixtures of d -mannitol polymorphs by FT-Raman spectroscopy and multivariate calibration models显示文摘 | Doris E. Braun Stephan G. Maas Neslihan Zencirci Christoph Langes Nora A. Urbanetz Ulrich J. Griesser | 2009 | International Journal of Pharmaceutics2009，，1： | 1 |
| 6 | Sufentanil does not block sympathetic responses to surgical stimuli in patients having coronary artery revascularization surgery显示文摘 | Sonntag H Stephan H Lange H | 1989 | Anesth Analg1989，68，： | 1 |
| 7 | Phenotypic and functional characteristics of CD4<sup>+</sup>CD39<sup>+</sup> FOXP3<sup>+</sup> and CD4<sup>+</sup>CD39<sup>+</sup>FOXP3<sup>neg</sup> T‐cell subsets in cancer patients显示文摘 | Patrick J. Schuler Bastian Schilling Malgorzata Harasymczuk Thomas K. Hoffmann Jonas Johnson Stephan Lang Theresa L. Whiteside | 2012 | Eur J Immunol2012，，7： | 1 |
| 8 | Expansion and characteristics of human T regulatory type 1 cells in co-cultures simulating tumor microenvironment显示文摘 | Christoph Bergmann Laura Strauss Reinhard Zeidler Stephan Lang Theresa L. Whiteside | 2007 | Cancer Immunology Immunotherapy2007，，9： | 1 |
| 9 | The WNT1^(G177C) mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV显示文摘The recent identification of homozygous WNT1 mutations in individuals with osteogenesis imperfecta type XV(OI-XV)has suggested that WNT1 is a key ligand promoting the differentiation and function of bone-forming osteoblasts.Although such aninfluence was supported by subsequent studies,a mouse model of OI-XV remained to be established.Therefore,we introduced a previously identified disease-causing mutation(G177C)into the murine Wnt1 gene.Homozygous Wnt1^(G177C/G177C)mice were viable and did not display defects in brain development,but the majority of 24-week-old Wnt1^(G177C/G177C)mice had skeletal fractures.This increased bone fragility was not fully explained by reduced bone mass but also by impaired bone matrix quality.Importantly,the homozygous presence of the G177C mutation did not interfere with the osteoanabolic influence of either parathyroid hormone injection or activating mutation of LRP5,the latter mimicking the effect of sclerostin neutralization.Finally,transcriptomic analyses revealed that short-term administration of WNT1 to osteogenic cells induced not only the expression of canonical WNT signaling targets but also the expression of genes encoding extracellular matrix modifiers.Taken together,our data demonstrate that regulating bone matrix quality is a primary function of WNT1.They further suggest that individuals with WNT1 mutations should profit from existing osteoanabolic therapies. | Nele Vollersen Wenbo Zhao Tim Rolvien Fabiola Lange Felix Nikolai Schmidt Stephan Sonntag Doron Shmerling Simon von Kroge Kilian Elia Stockhausen Ahmed Sharaf Michaela Schweizer Meliha Karsak Bjorn Busse Ernesto Bockamp Oliver Semler Michael Amling Ralf Oheim Thorsten Schinke Timur Alexander Yorgan | 2021 | Bone Research2021，9，4： | 1 |
| 10 | Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression显示文摘 | Magis Mandapathil Stephan Lang Elieser Gorelik Theresa L. Whiteside | 2009 | Journal of Immunological Methods2009，，1： | 1 |
| 11 | Anticoagulation and Survival in Pulmonary Arterial Hypertension: Results From the Comparative, Prospective Registry of Newly Initiated Therapies for Pulmonary Hypertension (COMPERA)显示文摘 | Karen M. Olsson Marion Delcroix H. Ardeschir Ghofrani Henning Tiede Doerte Huscher Rudolf Speich Ekkehard Grünig Gerd Staehler Stephan Rosenkranz Michael Halank Matthias Held Tobias J. Lange Juergen Behr Hans Klose Martin Claussen Ralf Ewert Christian F. | 2014 | Circulation2014，，1： | 1 |
| 12 | Sufentanil does not block sympa thetic responses to surgical stimuli in patients having coronary artery revascul arization surgery显示文摘 | Sonntag H Stephan H Lange H | 1989 | Anesth Analg1989，68，： | 1 |
| 13 | Recombinant alfa-interferon plus ribavirin therapy in children and adolescents with chronic hepatitis C显示文摘 | Stefan Wirth Thomas Lang Stephan Gehring Patrick Gerner | 2002 | Hepatology2002，，5： | 1 |
| 14 | Phenotypic and functional characteristics of CD4+CD39+ FOXP3+ and CD4+CD39+FOXP3neg T‐cell subsets in cancer patients显示文摘 | Patrick J. Schuler Bastian Schilling Malgorzata Harasymczuk Thomas K. Hoffmann Jonas Johnson Stephan Lang Theresa L. Whiteside | 2012 | Immunol2012，，7： | 1 |
| 15 | HMGB1 is overexpressed in tumor cells and promotes activity of regulatory T cells in patients with head and neck cancer显示文摘 | Clarissa A. Wild Sven Brandau Ramin Lotfi Stefan Mattheis Xiang Gu Stephan Lang Christoph Bergmann | 2011 | Oral Oncology2011，，5： | 1 |
| 16 | Allogenic antibody-mediated identification of head and neck cancer antigens显示文摘 | Jens Rauch Martin Ahlemann Martina Schaffrik Brigitte Mack Suna Ertongur Michaela Andratschke Reinhard Zeidler Stephan Lang Olivier Gires | 2004 | Biochemical and Biophysical Research Communications2004，，1： | 1 |
| 17 | Sufentanil does not block sympathetic responses to surreal stimuli in patients having coronary artery revaseniarization surgery显示文摘 | Sonntag H Stephan H Lange H | 2011 | Anesth Analg2011，68，7： | 1 |
| 18 | Minimally Invasive Versus Open Esophagectomy: Meta-Analysis of Outcomes显示文摘 | George Sgourakis Ines Gockel Arnold Radtke Thomas J. Musholt Stephan Timm Andreas Rink Achilleas Tsiamis Constantine Karaliotas Hauke Lang | 2010 | Digestive Diseases and Sciences2010，，11： | 1 |
| 19 | Cell Volume Regulatory Ion Channels in Cell Proliferation and Cell Death显示文摘 | Florian Lang Michael F?ller Karl Lang Philipp Lang Markus Ritter Alexey Vereninov Ildiko Szabo Stephan M. Huber Erich Gulbins | 2007 | Methods in Enzymology2007，，： | 1 |
| 20 | Clinical utility of anti‐glycan antibodies in pediatric crohn’s disease in comparison with an adult cohort显示文摘 | Florian Rieder Philipp Hahn Lydia Finsterhoelzl Stephan Schleder Alexandra Wolf Andrea Dirmeier Rocio Lopez Bo Shen Gerhard Rogler Frank Klebl Thomas Lang | 2011 | Inflamm Bowel Dis2011，，7： | 1 |
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