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| 1 | Establishment and Preliminary Application of a Rapid Fluorescent Focus Inhibition Test (RFFIT) for Rabies Virus显示文摘The World Health Organization (WHO) standard assay for determining levels of the rabies virus neutralization antibody (RVNA) is the rapid fluorescent focus inhibition test (RFFIT), which is used to evaluate the immunity effect after vaccination against rabies. For RFFIT, CVS-11 was used as the challenge virus, BSR cells as the adapted cells, and WHO rabies immunoglobulin (WHO STD) as the reference serum in this study. With reference to WHO and Pasteur RFFIT procedures, a micro-RFFIT procedure adapted to our laboratory was produced, and its specificity and reproducibility were tested. We tested levels of RVNA in human serum samples after immunization with different human rabies vaccines (domestic purified Vero cell rabies vaccine (PVRV) and imported purified chick embryo cell vaccine (PCECV)) using different regimens (Zagreb regimen and Essen regimen). We analyzed the levels of RVNA, and compared the immune efficacy of domestic PVRV and imported PCECV using different immunization regimens. The results showed that the immune efficacy of domestic PVRV using the Zagreb regimen was as good as that of the imported PCECV, but virus antibodies were generated more rapidly with the Zagreb regimen than with the Essen regimen. The RFFIT procedure established in our laboratory will enhance the comprehensive detection ability of institutions involved in rabies surveillance in China. | Pengcheng Yu Xinjun Lv Xinxin Shen Qing Tang Guodong Liang | 2013 | Virologica Sinica2013，28，4： | 11 |
| 2 | Development and validation of an endoscopic images-based deep learning model for detection with nasopharyngeal malignancies显示文摘Background:Due to the occult anatomic location of the nasopharynx and frequent presence of adenoid hyperpla-sia,the positive rate for malignancy identification during biopsy is low,thus leading to delayed or missed diagnosis for nasopharyngeal malignancies upon initial attempt.Here,we aimed to develop an artificial intelligence tool to detect nasopharyngeal malignancies under endoscopic examination based on deep learning.Methods:An endoscopic images-based nasopharyngeal malignancy detection model(eNPM-DM)consisting of a fully convolutional network based on the inception architecture was developed and fine-tuned using separate training and validation sets for both classification and segmentation.Briefly,a total of 28,966 qualified images were collected.Among these images,27,536 biopsy-proven images from 7951 individuals obtained from January 1st,2008,to December 31st,2016,were split into the training,validation and test sets at a ratio of 7:1:2 using simple randomiza-tion.Additionally,1430 images obtained from January 1st,2017,to March 31st,2017,were used as a prospective test set to compare the performance of the established model against oncologist evaluation.The dice similarity coef-ficient(DSC)was used to evaluate the efficiency of eNPM-DM in automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images,by comparing automatic segmentation with manual segmenta-tion performed by the experts.Results:All images were histopathologically confirmed,and included 5713(19.7%)normal control,19,107(66.0%)nasopharyngeal carcinoma(NPC),335(1.2%)NPC and 3811(13.2%)benign diseases.The eNPM-DM attained an overall accuracy of 88.7%(95%confidence interval(CI)87.8%-89.5%)in detecting malignancies in the test set.In the prospective comparison phase,eNPM-DM outperformed the experts:the overall accuracy was 88.0%(95%CI 86.1%-89.6%)vs.80.5%(95%CI 77.0%-84.0%).The eNPM-DM required less time(40 s vs.110.0±5.8 min)and exhibited encouraging performance in automatic segmentation of nasopharyngeal malignant area from the background,with an average DSC of 0.78±0.24 and 0.75±0.26 in the test and prospective test sets,respectively.Conclusions:The eNPM-DM outperformed oncologist evaluation in diagnostic classification of nasopharyngeal mass into benign versus malignant,and realized automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images. | Chaofeng Li Bingzhong Jing Liangru Ke Bin Li Weixiong Xia Caisheng He Chaonan Qian Chong Zhao Haiqiang Mai Mingyuan Chen Kajia Cao Haoyuan Mo Ling Guo Qiuyan Chen Linquan Tang Wenze Qiu Yahui Yu Hu Liang Xinjun Huang Guoying Liu Wangzhong Li Lin Wang Rui Sun Xiong Zou Shanshan Guo Peiyu Huang Donghua Luo Fang Qiu Yishan Wu Yijun Hua Kuiyuan Liu Shuhui Lv Jingjing Miao Yanqun Xiang Ying Sun Xiang Guo Xing Lv | 2018 | Cancer Communications2018，38，1： | 9 |
| 3 | A β-glucosidase-producing M-2 strain: Isolation from cow dung and fermentation parameter optimization for flaxseed cake显示文摘Flaxseed cake contains cyanogenic glucosides, which can be metabolized into hydrocyanic acid in an animal's body, leading to asphyxia poisoning in cells. Beta-glucosidase is highly efficient in degrading cyanogenic glucosides. The Cattle may have b-glucosidase-producing strains in the intestinal tract after eating small amounts of flaxseed cake for a long time. This study aimed to isolate of a strain from cow dung that produces b-glucosidase with high activity and can significantly reduce the amount of cyanogenic glucosides. We used cow dung as the microflora source and an esculin agar as the selective medium. After screening with 0.05% esculin and 0.01% ferric citrate, we isolated 5 strains producing high amounts of b-glucosidase. In vitro flaxseed cake fermentation was fermented by these 5 strains, in which the strain M-2 exerted the best effect(P < 0.05). The strain M-2 was identified as Lichtheimia ramosa and used as the fermentation strain to optimize the fermentation parameters by a single factor analysis and orthogonal experimental design. The optimum condition was as follows: inoculum size3%, water content 60%, time 144 h, and temperature 32℃. Under this condition, the removal rate of cyanogenic glucosides reached 89%, and crude protein increment reached 44%. These results provided a theoretical basis for the removal of cyanogenic glucosides in flaxseed and the comprehensive utilization of flaxseed cake. | Chenhui Li Junshu Wei Yaping Jing Baoxia Teng Pingrong Yang Xinjun Chen Haiying Huang Tang Zhao Tuanjie Che Chunjiang Zhang | 2019 | Animal Nutrition2019，，1： | 5 |
| 4 | Operational-space wrench and acceleration capability analysis for multi-link cable-driven robots显示文摘Multi-link cable-driven robots(MCDRs)have potential advantages in confined spaces exploration because of their redundancy and flexibility.Operational space wrench and acceleration capability analysis of MCDRs is important for their design,manipulability optimization,and motion planning.However,existing works mainly focus on capability analysis in the joint space.In this paper,we present a zonotope-based iterative method and a simplified capability zonotope to analyze the operational-space wrench and acceleration capability of MCDRs.In the iterative method,the capability generated by some cables can be iteratively added to the initial capability zonotope based on the Minkowski sum.In the simplified zonotope capability representation,a threshold is put forward to reduce redundant vertices and faces with little volume loss.Finally,simulations on a 24 DOFs MCDR are performed to verify the effectiveness of the developed method.The results demonstrate that our iterative algorithm can easily generate the capability zonotope with a few MB ROM,while traditional operational wrench capability evaluation without our iterative algorithm needs 18432 GB ROM.Furthermore,our simplified representation reduces the vertices and faces from 1260 and 2516 to 88 and 172,respectively,but with only 3.3%volume loss,which decreases the constraints of the robot and is conducive to manipulability optimization and motion planning. | SHENG XinJun TANG Lei HUANG XinJia ZHU LiMin ZHU XiangYang GU GuoYing | 2020 | Science China(Technological Sciences)2020，63，10： | 1 |
| 5 | Generation and characterization of neutralizing human recombinant antibodies against antigenic site II of rabies virus glycoprotein显示文摘 | Lina Sun Zhe Chen Li Yu Jingshuang Wei Chuan Li Jing Jin Xinxin Shen Xinjun Lv Qing Tang Dexin Li Mifang Liang | 2012 | Applied Microbiology and Biotechnology2012，，2： | 1 |
| 6 | Detection of genomic signatures for pig hairlessness using high-density SNP data显示文摘Hair provides thermal regulation for mammals and protects the skin from wounds,bites and ultraviolet(UV)radiation,and is important in adaptation to volatile environments.Pigs in nature are divided into hairy and hairless,which provide a good model for deciphering the molecular mechanisms of hairlessness.We conducted a genomic scan for genetically differentiated regions between hairy and hairless pigs using 60K SNP data,with the aim to better understand the genetic basis for the hairless phenotype in pigs.A total of 38405 SNPs in 498 animals from 36 diverse breeds were used to detect genomic signatures for pig hairlessness by estimating between-population(FST)values.Seven diversifying signatures between Yucatan hairless pig and hairy pigs were identified on pig chromosomes(SSC)1,3,7,8,10,11 and 16,and the biological functions of two notable genes,RGS17 and RB1,were revealed.When Mexican hairless pigs were contrasted with hairypigs,strong signatures were detected on SSC1 and SSC10,which harbor two functionally plausible genes,REV3L and BAMBI.KEGG pathway analysis showed a subset of overrepresented genes involved in the T cell receptor signaling pathway,MAPK signaling pathway and the tight junction pathways.All of these pathways may be important in local adaptability of hairless pigs.The potential mechanisms underlying the hairless phenotype in pigs are reported for the first time.RB1 and BAMBI are interesting candidate genes for the hairless phenotype in Yucatan hairless and Mexico hairless pigs,respectively.RGS17,REV3L,ICOS and RASGRP1 as well as other genes involved in the MAPK and T cell receptor signaling pathways may be important in environmental adaption by improved tolerance to UV damage in hairless pigs.These findings improve our understanding of the genetic basis for inherited hairlessness in pigs. | Ying SU Yi LONG Xinjun LIAO Huashui AI Zhiyan ZHANG Bin YANG Shijun XIAO Jianhong TANG Wenshui XIN Lusheng HUANG Jun REN Nengshui DING | 2014 | Frontiers of Agricultural Science and Engineering2014，1，4： | 0 |
| 7 | Gradient defects mediate negative thermal quenching in phosphors显示文摘Luminescent materials often suffer from thermal quenching(TQ),limiting the continuation of their applications under high temperatures up to 473 K.The formation of defect levels could suppress TQ,but rational synthesis and deep understanding of multiple defects-regulated luminescent materials working in such a wide temperature range still remain challenging.Here,we prepare a negative thermal quenching(NTQ)phosphor LiTaO_(3)∶Tb^(3+)by introducing gradient defects V_(Ta)^(5−),Tb_(Li)^(2+),and(V_(Ta)Tb_(Li))^(3−)as identified by advanced experimental and theoretical studies.Its photoluminescence significantly becomes intense with rising temperatures and then slowly increases at 373 to 473 K.The mechanism studies reveal that gradient defects with varied trapping depths could act as energy buffer layers to effectively capture the carriers.Under thermal disturbance,the stored carriers could successively migrate to the activators in consecutive and wide temperature zones,compensating for TQ to enhance luminescence emission.This study initiates the synthesis of multi-defect NTQ phosphors for temperature-dependent applications. | Mingxue Deng Xingzhong Cao Yangmin Tang Zhenzhen Zhou Lijia Liu Xiaofeng Liu Peng Zhang Lo-Yueh Chang Hao Ruan Xinjun Guo Jiacheng Wang Qian Liu | 2023 | Advanced Photonics2023，5，2： | 0 |
| 8 | 基于内镜图像深度学习的鼻咽恶性肿瘤检测模型的建立与验证显示文摘背景与目的由于鼻咽部解剖位置隐匿且腺体增生频发,活检时恶性肿瘤的阳性率较低,从而导致初诊时鼻咽恶性肿瘤确诊延时或漏诊。本文旨在建立一种人工智能工具——基于深度学习的内镜检查,来检测鼻咽恶性肿瘤。方法建立了一种基于内镜图像的鼻咽恶性肿瘤检测模型(endoscopic imagesbased nasopharyngeal malignancies detection model,eNPM-DM),该模型由基于空间结构的全卷积网络构成,采用单独训练集和验证集对分类和分割进行微调。总共收集了28,966张合格图像。其中,自2008年1月1日至2016年12月31日,从7951例个体中获得了27,536张经活检证实的图像,按照7∶1∶2的比例随机分为训练、验证和测试集。此外,将2017年1月1日到2017年3月31日获得的1430张图像纳入预测集,用以对建立模型的性能与肿瘤专家的评价进行比较。以鼻咽镜图像为背景,对自动分割和专家手工分割进行比较,采用dice相似系数(dice similarity coefficient,DSC)评价eNPM-DM从鼻咽部内镜图像的背景中自动分割出恶性肿瘤区域的效率。结果所有图像经过病理组织学验证,包括正常对照5713(19.7%)例、鼻咽癌(nasopharyngeal carcinoma,NPC)19,107(66.0%)例、其他恶性肿瘤335(1.2%)例和3811(13.2%)例良性病变。在测试集中,eNPM-DM检测恶性肿瘤的总准确率达88.7%[95%置信区间(confidence interval,CI):87.8%–89.5%]。在预测比较阶段,eNPM-DM表现优于专家:总准确率分别为88.0%(95%CI:86.1%–89.6%)和80.5%(95%CI:77.0%–84.0%)。eNPM-DM耗时更短(40 s vs. 110.0±5.8 min),且从背景中自动分割出鼻咽恶性肿瘤区域方面表现优秀,测试集和预测集中的平均DSC分别为0.78±0.24和0.75±0.26。结论 eNPM-DM在鼻咽肿块良性/恶性诊断分类方面优于肿瘤学家评估,并且实现了从鼻咽内镜图像背景中对恶性区域自动分割。 | Chaofeng Li Bingzhong Jing Liangru Ke Bin Li Weixiong Xia Caisheng He Chaonan Qian Chong Zhao Haiqiang Mai Mingyuan Chen Kajia Cao Haoyuan Mo Ling Guo Qiuyan Chen Linquan Tang Wenze Qiu Yahui Yu Hu Liang Xinjun Huang Guoying Liu Wangzhong Li Lin Wang Rui Sun Xiong Zou Shanshan Guo Peiyu Huang Donghua Luo Fang Qiu Yishan Wu Yijun Hua Kuiyuan Liu Shuhui Lv Jingjing Miao Yanqun Xiang Ying Sun Xiang Guo Xing Lv | 2019 | 癌症2019，38，7： | 0 |
| 9 | A dual-targeted multifunctional nanoformulation for potential prevention and therapy of Alzheimer’s disease显示文摘Antioxidation and adjustable treatment strategies are critical for the effective treatment of Alzheimer’s disease(AD).Here,we design a dual-targeted Prussian blue nanoformulation(PTCN)that can cross the blood-brain barrier and target amyloid beta aggregates further exert antioxidant effects.An adjustable gradient dosing strategy with PTCN is used for the first time to design the preventive and therapeutic trials based on the severity of oxidative stress at different AD stages.The results show that PTCN could effectively ameliorate AD-related pathological processes,improve the cognitive decline,and rescue hippocampal atrophy of APP/PS1 mice in both preventive and therapeutic trials.Altogether,PTCN provided here is a successful combination of three traditional biomaterials with good biosafety,which has broad prospects for the early prevention,mild remission,and late treatment of AD,and is expected to be developed into personalized therapeutic drugs and healthcare products for clinical AD in the future. | Dongju Zhao Yuqing Tang Xinjun Suo Chaonan Zhang Yan Dou Jin Chang | 2021 | The Innovation2021，2，4： | 0 |
| 10 | Xuebijing alleviates LPS-induced acute lung injury by downregulating pro-inflammatory cytokine production and inhibiting gasdermin-E-mediated pyroptosis of alveolar epithelial cells显示文摘Acute lung injury/acute respiratory distress syndrome(ALI/ARDS)is characterized by diffuse alveolar injury primarily caused by an excessive inflammatory response.Regrettably,the lack of effective pharmacotherapy currently available contributes to the high mortality rate in patients with this condition.Xuebijing(XBJ),a traditional Chinese medicine recognized for its potent anti-inflammatory properties,exhibits promise as a potential therapeutic agent for ALI/ARDS.This study aimed to explore the preventive effects of XBJ on ALI and its underlying mechanism.To this end,we established an LPS-induced ALI model and treated ALI mice with XBJ.Our results demonstrated that pre-treatment with XBJ significantly alleviated lung inflammation and increased the survival rate of ALI mice by 37.5%.Moreover,XBJ substantially suppressed the production of TNF-α,IL-6,and IL-1βin the lung tissue.Subsequently,we performed a network pharmacology analysis and identified identified 109 potential target genes of XBJ that were mainly involved in multiple signaling pathways related to programmed cell death and anti-inflammatory responses.Furthermore,we found that XBJ exerted its inhibitory effect on gasdermin-E-mediated pyroptosis of lung cells by suppressing TNF-αproduction.Therefore,this study not only establishes the preventive efficacy of XBJ in ALI but also reveals its role in protecting alveolar epithelial cells against gasdermin-E-mediated pyroptosis by reducing TNF-αrelease. | ZHANG Cuiping CHEN Xiaoyan WEI Tianchang SONG Juan TANG Xinjun BI Jing CHEN Cuicui ZHOU Jian SU Xiao SONG Yuanlin | 2023 | Chinese Journal of Natural Medicines2023，21，8： | 0 |
| 11 | High-performance nonfused ring electron acceptor with a steric hindrance induced planar molecular backbone显示文摘Three nonfused ring electron acceptors (NFREAs) TTC6,TT-C8T and TT-TC8 were purposefully designed and synthesized.The molecular geometry can be adjusted by the steric hindrance of lateral substituents.According to the DFT calculations,from TTC6 to TT-C8T and TT-TC8,planarity of the molecular backbone is gradually improved,accompanying with the enhancing of intramolecular charge transfer effect.As for TT-TC8,the two phenyl substituents are almost perpendicular to the molecular backbone,which endues the acceptor with good solubility and suppresses it to form over-aggregation.Multidirectional regular molecular orientation and closer molecular stacking are formed in TT-TC8 film.As a result,TT-TC8 based devices afford the highest PCE of 13.13%,which is much higher than that of TTC6 (4.41%) and TT-C8T (10.42%) and among the highest PCE values based on NFREAs. | Hao Lu Xiaodong Wang Hang Wang Andong Zhang Xinming Zheng Na Yu Zheng Tang Xinjun Xu Yahui Liu Ya-Nan Chen Zhishan Bo | 2022 | Science China Chemistry2022，65，3： | 0 |
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