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| 1 | Effects of Salvia miltiorrhiza on intestinal microflora in rats with ischemia/reperfusion liver injury显示文摘BACKGROUND: Hepatic ischemia/reperfusion injury may induce intestinal microflora imbalance. Salvia miltiorrhiza is effective in promoting blood circulation and counteracting peroxidation in tissues. The aim of the present study was to determine the effects of Salvia miltiorrhiza on intestinal mi- croflora, endotoxemia, and bacterial translocation in rats with hepatic I/R injury. METHODS: Sprague-Dawley rats in specific pathogen free grade were divided into 3 groups: group I(n =6) for sham operation: groups ( n = 7) for liver ische- mia for 20 minutes and reperfusion for 22 hours. Group was also pretreated with 4 ml/day of Salvia miltiorrhiza solu- tion (250 mg/kg) by daily gavage for 7 days. The levels of serum alanine aminotransferase (ALT), aspartate amino- transferase (AST), malondialdehyde ( MDA) and supero- xide dismutase ( SOD ) in liver tissues, serum endotoxin, intestinal bacterial counts, intestinal mucosal histology and bacterial translocation were studied. RESULTS: The levels of ALT, AST, plasma endotoxin and MDA in liver tissues were decreased more markedly in group (57.57 ± 18.08 U/L, 147.57 ±40.84 U/L, 0.42 ± 0.144 EU/ml and 0. 52 ±0.19 nmol/mg-prot respectively) in group 295.9±216.92 U/L, 0.80± 0.262 EU/ml and 0.72±0.12 nmol/mg-prot; P <0.05-0.01 respectively). Liver SOD activity was increased more sig- nificantly in group (318.47±64.62 U/mg-prot) than in group U/mg-prot, P<0.05). The counts of Bifidobacteria and Bacteroides increased more significantly in group than in group but were similar to those in group I. Bacterial translocation to the kidney in group was 50% (5/10), whereas no bacterial translocation to the kidney occurred in the other two groups (P <0. 01). Ileal mucosal structure was markedly ameliorated in group as compared with group CONCLUSIONS: Salviae miltiorrhiza could partially restore intestinal microflora balance, improve intestinal mucosal integrity, and reduce bacterial translocation and plasma en- dotoxin in rats with hepatic ischemia/reperfusion injury. | Hui-Chun Xing, Lan-Juan Li, Kai-Jin Xu, Tian Shen, Yun-Bo Chen, Yu Chen, Su-Zhen Fu, Ji-Fang Sheng, Chun-Lei Chen, Jian-Guo Wang, Dong Yan, Fang-Wei Dai and Xiao-Ying Sha Hangzhou, China Key Laboratory of Infectious Diseases, Ministry of Public Health of China, and Institute of Infectious Diseases, Department of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Zhejiang Academy of Medical Sciences, Hangzhou 310012, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 14 |
| 2 | TNF-α suppresses sweat gland differentiation of MSCs by reducing FTO-mediated m^6 A-demethylation of Nanog mRNA显示文摘An effect of inhibition of tumor necrosis factor-α(TNF-α)on differentiation of mesenchymal stromal cells(MSCs)has been demonstrated,but the exact mechanisms that govern MSCs differentiation remain to be further elucidated.Here,we show that TNF-αinhibits the differentiation of MSCs to sweat glands in a specific sweat gland-inducing environment,accompanied with reduced expression of Nanog,a core pluripotency factor.We elucidated that fat mass and obesity-associated protein(FTO)-mediated m^6 A demethylation is involved in the regulation of MSCs differentiation potential.Exposure of MSCs to TNF-αreduced expression of FTO,which demethylated Nanog m RNA.Reduced expression of FTO increased Nanog m RNA methylation,decreased Nanog m RNA and protein expression,and significantly inhibited MSCs capacity for differentiation to sweat gland cells.Our finding is the first to elucidate the functional importance of m^6 A modification in MSCs,providing new insights that the microenvironment can regulate the multipotency of MSCs at the post-transcriptional level.Moreover,to maintain differentiation capacity of MSCs by regulating m^6 A modification suggested a novel potential therapeutic target for stem cellmediated regenerative medicine. | Yihui Wang Rui Wang Bin Yao Tian Hu Zhao Li Yufan Liu Xiaoli Cui Liuhanghang Cheng Wei Song Sha Huang Xiaobing Fu | 2020 | Science China(Life Sciences)2020，63，1： | 9 |
| 3 | Numerical Analysis of Space Charge Characteristics in Low-density Polyethylene Nanocomposite Under External DC Electric Field显示文摘The dynamic characteristics of space charge in nanocomposite of low-density polyethylene (LDPE) mixed with inorganic nano- fillers. Different from previous qualitatively theoretical works, we investigated the influence of trap depth, trap volume charge density and injection barrier height on the space charge and the electric field distribution in the nanocomposite under a DC external electric field (100 MV/m) systematically and quantificationally, through numerical simulations based on a bipolar charge transport model. The simulation re- sults showed that, the increase of trap depth would severely distort the balanced electric field distribution with the highest and the lowest electric field of 119 MV/m and 47 MV/m, respectively. It is concluded that the introduction of nanofillers creates more deep traps, which would block space charges by capturing most of them in the vicinity of electrode and hence reduce the local electric field largely. Further theoretical analysis of the simulation indicates that both the high permittivity and the low local electric field of the nanocomposite contri- buted to the increase of injection barrier height, and almost no charge could overcome an injection barrier higher than 1.25 eV. At last, a mechanism of space charge suppression in the LDPE nanocomposite was presented. | ZHOU Yuanxiang ZHANG Ling SHA Yanchao TIAN Jihuan | 2013 | 高电压技术2013，39，8： | 6 |
| 4 | In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids显示文摘Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients. | Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha | 2020 | Cell Research2020，30，3： | 6 |
| 5 | A fully automatic registration approach based on contour and SIFT for HJ-1 images显示文摘To achieve a fully automatic registration between HJ-1 CCD images and HJ-1 infrared images is a difficult task as it must deal with the varying illuminations and resolutions of the images,different perspectives,and the local deformations within the images.In this paper,aimed at those registration issues,a fully automatic registration approach based on contour and SIFT is proposed.The registration technique performs a pre-registration process using contour feature matching algorithm that decides the overlapping region between a reference image and an input image.Once the coarse regions are obtained,it performs a fine registration process based on SIFT detector and a local adaptive matching strategy.In the fine registration process,image blocking theory is used,which not only speeds up the features extraction and matching,but also makes the matching point pairs distributed uniformly in images,and further improves the accuracy of input image rectification.Experiments with visible images and infrared images from HJ-1A/B demonstrate the efficiency and the accuracy of the proposed technique for multisource remote sensing images registration. | NI XiLiang CAO ChunXiang DING Lin JIANG Tao ZHANG Hao JIA HuiCong LI GuangHe ZHAO Jian CHEN Wei JI Wei XU Min GAO MengXu ZHENG Sheng TIAN Rong LIU Cheng LI Sha | 2012 | Science China Earth Sciences2012，55，10： | 5 |
| 6 | Systematic analyses of glutamine and glutamate metabolisms across different cancer types显示文摘Background: Glutamine and glutamate are known to play important roles in cancer biology. However, no detailed information is available in terms of their levels of involvement in various biological processes across different cancer types, whereas such knowledge could be critical for understanding the distinct characteristics of different cancer types. Our computational study aimed to examine the functional roles of glutamine and glutamate across different cancer types.Methods: We conducted a comparative analysis of gene expression data of cancer tissues versus normal control tissues of 11 cancer types to understand glutamine and glutamate metabolisms in cancer. Specifically, we developed a linear regression model to assess differential contributions by glutamine and/or glutamate to each of seven biological processes in cancer versus control tissues.Results: While our computational predictions were consistent with some of the previous observations, multiple novel predictions were made:(1) glutamine is generally not involved in purine synthesis in cancer except for breast cancer, and is similarly not involved in pyridine synthesis except for kidney cancer;(2) glutamine is generally not involved in ATP production in cancer;(3) glutamine's contribution to nucleotide synthesis is minimal if any in cancer;(4) glutamine is not involved in asparagine synthesis in cancer except for bladder and lung cancers; and(5) glutamate does not contribute to serine synthesis except for bladder cancer.Conclusions: We comprehensively predicted the roles of glutamine and glutamate metabolisms in selected metabolic pathways in cancer tissues versus control tissues, which may lead to novel approaches to therapeutic development targeted at glutamine and/or glutamate metabolism. However, our predictions need further functional validation. | Yuan Tian Wei Du Sha Cao Yue Wu Ning Dong Yan Wang Ying Xu | 2017 | Chinese Journal of Cancer2017，36，12： | 5 |
| 7 | Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment显示文摘Background:Mammary progenitor cells(MPCs)maintain their reproductive potency through life,and their specific microenvironments exert a deterministic control over these cells.MPCs provides one kind of ideal tools for studying engineered microenvironmental influence because of its accessibility and continually undergoes postnatal developmental changes.The aim of our study is to explore the critical role of the engineered sweat gland(SG)microenvironment in reprogramming MPCs into functional SG cells.Methods:We have utilized a three-dimensional(3D)SG microenvironment composed of gelatin-alginate hydrogels and components from mouse SG extracellular matrix(SG-ECM)proteins to reroute the differentiation of MPCs to study the functions of this microenvironment.MPCs were encapsulated into the artificial SG microenvironment and were printed into a 3D cell-laden construct.The expression of specific markers at the protein and gene levels was detected after cultured 14 days.Results:Compared with the control group,immunofluorescence and gene expression assay demonstrated that MPCs encapsulated in the bioprinted 3D-SG microenvironment could significantly express the functional marker of mouse SG,sodium/potassium channel protein ATP1a1,and tend to express the specific marker of luminal epithelial cells,keratin-8.When the Shh pathway is inhibited,the expression of SG-associated proteins in MPCs under the same induction environment is significantly reduced.Conclusions:Our evidence proved the ability of differentiated mouse MPCs to regenerate SG cells by engineered SG microenvironment in vitro and Shh pathway was found to be correlated with the changes in the differentiation.These results provide insights into regeneration of damaged SG by MPCs and the role of the engineered microenvironment in reprogramming cell fate. | Rui Wang Yihui Wang Bin Yao Tian Hu Zhao Li Yufan Liu Xiaoli Cui Liuhanghang Cheng Wei Song Sha Huang Xiaobing Fu | 2019 | Burns & Trauma2019，7，1： | 5 |
| 8 | The potential value of microRNA-4463 in the prognosis evaluation in hepatocellular carcinoma显示文摘The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma(HCC),and to clarify the meaning of them in the prognosis evaluation in HCC.Forty-five samples from healthy people and patients,who had been diagnosed with hepatocellular carcinoma before any treatment,were collected to study respectively.Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients.The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group(P<0.05),and the expression level was independent of gender,tumor size,cell types,stages,alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and HBsAg status(P>0.05).But there was a significant difference of different level of AFP in HCC(P<0.05),and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant(P<0.05).Besides,the survival time had showed a significant difference at the high and low expression levels(P<0.05).But the expression level of miRNA-6087 was no difference in HCC and control group.The disorder of miRNA-4463 occurred in HCC,even the AFP level doesn’t rises.What’s more,patients who get the high level of miRNA-4463 seem to have a shorter survival time.And it contributes great to the prognostic evaluation.This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC. | Tian Hu Jincheng Li Chuhong Zhang Xiu lv Sai Li Sha He Hanxing Yan Yixi Tan Mingsheng Lei Meiling Wen Jianhong Zuo | 2017 | Genes & Diseases2017，4，2： | 3 |
| 9 | Rapid intelligent evaluation method and technology for determining engineering rock mass quality显示文摘The evaluation of engineering rock mass quality is fundamental work for the engineering activities of rock mass.The increasing scale of rock mass engineering necessitates higher intelligence,timeliness,and accuracy in engineering rock mass quality evaluation.As the core aspects of engineering rock mass quality evaluation,the structural characteristics,mechanical characteristics,and quality classification of rock mass have been innovated in recent years.The non-contact measurement technology for rock mass structure and rapid interpretation of rock mass structure information enables the intelligent extraction and analysis of rock mass structure parameters.The modular backpack laboratory system of rock mechanics provides an effective means to acquire rock mechanical parameters on-site conveniently.The theory of statistical mechanics of rock mass(SMRM)integrates various factors such as the rock mass properties,geological environment,and engineering disturbance,providing a theoretical basis for accurately evaluating the weakening and anisotropy of rock mass.The cloud computing platform established based on SMRM can provide technical support for the rapid calculation of rock mass parameters and instant evaluation of the rock mass quality.The development of intelligent evaluation method and technology is altering the conventional technical state of qualitative and semi-quantitative evaluation of engineering rock mass quality,supporting the realization of rock mass engineering construction with intellectualization and informatization. | Faquan Wu Jie Wu Han Bao Zhongxi Bai Lei Qiao Fang Zhang Bo Li Fuan Si Lei Yu Shenggong Guan Peng Sha Deheng Kong Zhenzhong Dai Kun Chen Yun Tian Changqing Liu | 2023 | Rock Mechanics Bulletin2023，2，2： | 3 |
| 10 | Investigation of Coal Fueled Chemical Looping Combustion Using Fe_3O_4 as Oxygen Carrier:Influence of Variables显示文摘Chemical-looping combustion (CLC) is a novel combustion technique with inherent CO2 separation.Magnetite (Fe3O4) was selected as the oxygen carrier.Shenhua coal (Inner Mongolia,China),straw coke and natural coke were used as fuels for this study.Influences of operation temperatures,coal to Fe3O4 mass ratios,and different kinds of fuels on the reduction characteristics of the oxygen carrier were investigated using an atmosphere thermogravimetric analyzer (TGA).Scanning electron microscopy (SEM) was used to analyse the characteristic of the solid residues.Experimental results shown that the reaction between the coal and the oxygen carrier become strong at a temperature of higher than 800℃.As the operation temperature rises,the reduction conversion rate increases.At the temperatures of 850oС,900℃,and 950℃,the reduction conversion rates were 37.1%,46.5%,and 54.1% respectively.However,SEM images show that at the temperature of higher than 950℃,the iron oxides become melted and sintered.The possible operation temperature should be kept around 900℃.When the mass ratios of coal to Fe3O4 were 5/95,10/90,15/85,and 20/80,the reduction conversion rates were 29.5%,40.8%,46.5%,and 46.6% respectively.With the increase of coal,the conversion rate goes up.But there exist an optimal ratio around 15/85.Comparisons based on different kinds of fuels show that the solid fuel with a higher volatile and a more developed pore structure is conducive to the reduction reactivity of the oxygen carrier. | Xiaoyan Sun Wenguo Xiang Sha Wang Wendong Tian Xiang Xu Yanji Xu Yunhan Xiao | 2010 | Journal of Thermal Science2010，19，3： | 3 |
| 11 | 3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking显示文摘3-dimensional(3D)bioprinting technology provides promising strategy in the fabrication of artificial tissues and organs.As the fundamental element in bioprinting process,preparation of bioink with ideal mechanical properties without sacrifice of biocompatibility is a great challenge.In this study,a supramolecular hydrogel-based bioink is prepared by polyethylene glycol(PEG)grafted chitosan,α-cyclodextrin(α-CD)and gelatin.It has a primary crosslinking structure through the aggregation of the pseudo-polyrotaxane-like side chains,which are formed from the host-guest interactions betweenα-CD and PEG side chain.Apparent viscosity measurement shows the shear-shinning property of this bioink,which might be due to the reversibility of the physical crosslinking.Moreover,withβ-glycerophosphate at different concentrations as the secondary crosslinking agent,the printed constructs demonstrate different Young's modulus(p<0.001).They could also maintain the Young's modulus in cell culture condition for at least 21 days(p<0.05).By co-culturing each component with fibroblasts,CCK-8 assay demonstrate cellular viability is higher than 80%.After bioprinting and culturing,immunofluorescence staining with quantification indicate the expression of Ki-67,Paxillin,and N-cadherin is higher in day 14 than those in day 3(p<0.05).Oil red O and Nissl body specific staining reflect strength tunable bioink may have impact on the cell fate of mesenchymal stem cells(p<0.05).This work might provide new idea for advanced bioink in the application of re-establishing complicated tissues and organs. | Tian Hu Xiaoliang Cui Meng Zhu Man Wu Ye Tian Bin Yao Wei Song Zhongwei Niu Sha Huang Xiaobing Fu | 2020 | Bioactive Materials2020，5，4： | 3 |
| 12 | Microbiota analysis revealed vertical transmission and microbial adjusting function of symbiotic fungus in the attelabid weevil fungiculture显示文摘Dear Editor,Microbial symbionts are crucial participants in the life history of fungus-farming insects (Mueller et al., 2005;Youngsteadt, 2008). The attelabid weevil (Euops chinensis)feeding on Fallopia japonica in southern China, has evolved a special proto-farming bipartite symbiosis with the fungus(Penicillium herquei), which produces antibiotic (+)-scleroderolide in the leaf-rolls ('cradle' for offspring development) to protect the offspring from potential microbial parasites and pathogens (Sakurai, 1985;Wang et al., 2015). | Bin Lin Lin Wang Muzammi Hussain Xiaoling Zhang Jianqing Tian Sha Qi Xingzhong Liu Meichun Xiang | 2019 | Science China(Life Sciences)2019，62，12： | 3 |
| 13 | Preparation of New Cementitious System using Fly Ash and Dehydrated Autoclaved Aerated Concrete显示文摘We experimentally studied the interaction between pozzolanic material(fly ash) and dehydrated autoclaved aerated concrete(DAAC). The DAAC powder was obtained by grinding aerated concrete waste to particles fi ner than 75μm and was then heated to temperatures up to 900 ℃. New cementitious material was prepared by proportioning fly ash and DAAC, named as AF. X-ray diffraction(XRD) was employed to identify the crystalline phases of DAAC before and after rehydration. The hydration process of AF was analyzed by the heat of hydration and non-evaporable water content(Wn). The experimental results show that the highest reactivity of DAAC can be obtained by calcining the powder at 700 ℃ and the dehydrated products are mainly β-C2 S and CaO. The cumulative heat of hydration and Wn was found to be strongly dependent on the replacement level of fl y ash, increasing the replacement level of fl y ash lowered them in AF. The strength contribution rates on pozzolanic effect of fl y ash in AF are always negative, showing a contrary tendency of that of cement-fl y ash system. | 水中和 LU Jianxin TIAN Sufang SHEN Peiliang DING Sha | 2014 | Journal of Wuhan University of Technology(Materials Science)2014，29，4： | 3 |
| 14 | Establishment of a highly efficient hematopoietic differentiation model from human embryonic stem cells for functional screening显示文摘Human embryonic stem cells(hESCs)have been successfully differentiated into hematopoietic progenitor cells with colony formation capacity and further into various kinds of blood cells including erythrocytes,megakaryocytes,neutrophils,nature killer cells and T lymphocytes[1 7].Nevertheless,the differentiation efficiency is extremely low. | PANG SuLei WU QingQing TIAN Sha SU Pei BAI Yang GAO Jie YANG YiQing LIU Xin ZHU ZhengMao XU YuanFu ZHOU JiaXi | 2013 | Science China(Life Sciences)2013，56，12： | 3 |
| 15 | The role of hypoxia-inducible factors in tumor angiogenesis and cell metabolism显示文摘Hypoxia-inducible factor(HIF)is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible genes.HIF is frequently upregulated in solid tumors,and the overexpression of HIF can promote tumor progression or aggressiveness by blood vessel architecture and altering cellular metabolism.In this review,we focused on the pivotal role of HIF in tumor angiogenesis and energy metabolism.Furthermore,we also emphasized the possibility of HIF pathway as a potential therapeutic target in cancer. | Xiu Lv Jincheng Li Chuhong Zhang Tian Hu Sai Li Sha He Hanxing Yan Yixi Tan Mingsheng Lei Meiling Wen Jianhong Zuo | 2017 | Genes & Diseases2017，4，1： | 3 |
| 16 | Shear wave splitting analysis of local earthquakes from dense arrays in Shimian,Sichuan显示文摘The Shimian area of Sichuan sits at the junction of the Bayan Har block.Sichuan-Yunnan rhombic block,and Yangtze block,where several faults intersect.This region features intense tectonic activity and frequent earthquakes.In this study,we used local seismic waveform data recorded using dense arrays deployed in the Shimian area to obtain the shear wave splitting parameters at 55 seismic stations and thereby determine the crustal anisotropic characteristics of the region.We then analyzed the crustal stress pattern and tectonic setting and explored their relationship in the study area.Although some stations returned a polarization direction of NNW-SSE.a dominant polarization direction of NW-SE was obtained for the fast shear wave at most seismic stations in the study area.The polarization directions of the fast shear wave were highly consistent throughout the study-area.This orientation was in accordance with the direction of the regional principal compressive stress and parallel to the trend of the Xianshuihe and Daliangshan faults.The distribution of crustal anisotropy in this area was affected by the regional tectonic stress field and the fault structures.The mean delay time between fast and slow shear waves was 3.83 ms/km.slightly greater than the values obtained in other regions of Sichuan.This indicates that the crustal media in our study area had a high anisotropic strength and also reveals the influence of tectonic complexity resulting from the intersection of multiple faults on the strength of seismic anisotropy. | Sha Liu Baofeng Tian | 2023 | Earthquake Science2023，36，1： | 2 |
| 17 | A Method for Eliminating the Impact of Microwave Sweeper Power Fluctuation in BOTDA System显示文摘在 BOTDA 系统的微波 sweeper 电源变化的影响理论上被分析了并且试验性地测试了。并且把探查波浪电源的即时获得与一个新算法作比较为消除这影响认识到探查波浪电源正规化的一个新奇方法被建议。建议方法的原则理论上被描述。并且在我们的新方法和常规方法之间的对比测试被执行。实验结果显示温度精确性有效地被改进从 ? | Shuo Jiang Jun Chang Sasa Zhang Zongliang Wang Sha Luo Boning Sun Xiaohui Liu Yongning Liu Junqiang Tian Chuanwu Jia | 2014 | Photonic Sensors2014，4，1： | 2 |
| 18 | Results of Clinical Trial of Superficially-placed Tumor Therapy with Carbon Ions at Heavy Ion Research Facility in Lanzhou(HIRFL显示文摘 | Zhang Hong Li Sha Wang Xiaohu Li Qiang Zhao Weiping Dai Zhongying Wei Shihua Gao Liying Xiao Guoqing Xu Hushan Hu Zhengguo Mao Ruishi Yue Yangjun Ran Juntao Tian Zhongze Song Mingtao Zhao Hongwei Zhan Wenlong | 2007 | 近代物理研究所和兰州重离子加速器实验室年报:英文版2007，，1： | 2 |
| 19 | Genome-wide identification of chitin-binding proteins and characterization of BmCBP1 in the silkworm, Bombyx mori显示文摘The insect cuticle plays important roles in numerous physiological functions to protect the body from invasion of pathogens, physical injury and dehydration. In this report, we conducted a comprehensive genome-wide search for genes encoding proteins with peritrophin A-type (ChtBD2) chitin-binding domain (CBD) in the silkworm, Bombyx mori. One of these genes, which encodes the cuticle protein BmCBP1, was additionally cloned, and its expression and location during the process of development and molting in B. mori were investigated. In total, 46 protein-coding genes were identified in the silkworm genome, including those encoding 15 cuticle proteins analogous to peritrophins with one CBD (CPAP1s), nine cuticle proteins analogous to peritrophins with three CBD (CPAP3s), 15 peritrophic membrane proteins (PMPs), four chitinases, and three chitin deacetylases, which contained at least one ChtBD2 domain. Microarray analysis indicated that CPAP-encoding genes were widely expressed in various tissues, whereas PMP genes were highly expressed in the midgut. Quantitative polymerase chain reaction and western blotting showed that the cuticle protein BmCBP1 was highly expressed in the epidermis and head, particularly during molting and metamorphosis. An immunofluorescence study revealed that chitin co-localized with BmCBP1 at the epidermal surface during molting. Additionally, BmCBP1 was notably up-regulated by 20-hydroxyecdysone treatment. These results provide a genome-level view of the chitin-binding protein in silkworm and suggest that BmCBP1 participates in the formation of the new cuticle during molting. | Zhi-Lang Li Sha Tian Huan Yang Xia Zhou Shu-Ping Xu Zi-Yu Zhang Jing Gong Yong Hou Qing-You Xia | 2019 | Insect Science2019，26，3： | 2 |
| 20 | Scaled-up synthesis of defect-rich layered double hydroxide monolayers without organic species for efficient oxygen evolution reaction显示文摘The scaled-up synthesis of organic-free monolayer nanomaterials is highly desirable,especially in obtaining green energy by electrocatalysis.In this study,a method for the scaled-up synthesis of the series of monolayer layered double hydroxides(LDHs)without the addition of organic solvents is reported via the separate nucleation and aging steps process.The resulting monolayer LDHs with the thicknesses of less than 1 nm showed a narrow thickness distribution.X-ray absorption fine-structure revealed that monolayer NiFe-LDH nanosheets have a number of oxygen and metal vacancies defects.As a practical application,monolayer NiFe-LDH nanosheets containing defects showed an enhanced electrocatalytic water oxidation activity compared with that of bulk NiFe-LDH.Density functional theory calculations uncovered that excellent catalytic activity is attributed to vacancies defects.The proposed method is an economical and universally applicable strategy for the scaled-up production of monolayer LDHs. | Haoyuan Chi Jingwen Dong Tian Li Sha Bai Ling Tan Jikang Wang Tianyang Shen Guihao Liu Lihong Liu Luyi Sun Yufei Zhao Yu-Fei Song | 2022 | Green Energy & Environment2022，7，5： | 2 |
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