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| 1 | Effects of dietary protein/energy ratio on growth performance,carcass trait,meat quality,and plasma metabolites in pigs of different genotypes显示文摘Background:The protein/energy ratio is important for the production performance and utilization of available feed resources by animals.Increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment.This study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance,carcass traits,meat quality,and plasma metabolites of pigs of different genotypes.Methods:Bama mini-pigs and Landrace pigs were randomly assigned to two dietary treatment groups(Chinese conventional diet with low protein/energy ratio or National Research Council diet with high protein/energy ratio;n = 24 per treatment) in a 2x2 factorial arrangement.Blood and muscle samples were collected at the end of the nursery,growing,and finishing phases.Results:We observed significant interactions(P< 0.05) between breed and diet for total fat percentage,intramuscular fat(IMF) content,protein content in biceps femoris(BF) muscle,and plasma urea nitrogen(UN) concentration in the nursery phase;for average daily gain(ADG),average daily feed intake(ADFI),dry matter,IMF content in psoas major(PM) muscle,and plasma total protein and albumin concentrations in the growing phase;and for drip loss and plasma UN concentration in the finishing phase.Breed influenced(P < 0.05) growth performance,carcass traits,and meat quality,but not plasma metabolites.Throughout the trial,Landrace pigs showed significantly higher(P < 0.05) ADG,ADFI,dressing percentage,lean mass rate,and loin-eye area than did Bama mini-pigs,but significantly lower(P< 0.05)feed/gain ratio,fat percentage,backfat thickness,and IMF content.Dietary protein/energy ratio influenced the pH value,chemical composition of BF and PM muscles,and plasma activities of glutamic-pyruvic transaminase and gamma-glutamyl transpeptidase,and plasma concentration of UN.Conclusions:Compared with Landrace pigs,Bama mini-pigs showed slower growth and lower carcass performance,but had better meat quality.Moreover,unlike Landrace pigs,the dietary protein/energy ratio did not affect the growth performance of Bama mini-pigs.These results suggest that,in swine production,low dietary protein/energy ratio may be useful for reducing feed costs and minimizing the adverse effects of ammonia release into the environment. | Yingying Liu Xiangfeng Kong Guoli Jiang Bi'e Tan Jinping Deng Xiaojian Yang Fengna Li Xia Xiong Yulong Yin | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 37 |
| 2 | Nat Genet:单细胞分析解开结直肠癌细胞的神秘面纱显示文摘结合单细胞基因组学和计算机技术,一个研究团队(包括来自杰克逊实验室(JAx)单细胞生物学主任Paul Robson博士在内)鉴定出了11种结直肠癌肿瘤的癌细胞组成及邻近的非癌细胞。这对于更好的肿瘤靶向诊断和治疗很重要。”使用单细胞测序。”JAX科学家、这篇发表在Nature Genetics上的文章共同第一作者Elise Courtois说道。”我们可以根据肿瘤中的细胞组成将结直肠癌进一步分类。因为每种亚型的肿瘤病人生存率存在差别,我们的方法将为肿瘤医生提供更多的关于肿瘤预后和治疗的信息。” | Huipeng Li, Elise T Courtois, Debarka Sengupta, Yuliana Tan Shyam Prabhakar Elise T Courtois, Yuliana Tan Paul Robson Debarka Sengupta Kok Hao Chen, Jolene Jie Lin Goh Paul Jongjoon Choi Say Li Kong, Axel M Hillmer Iain Beehuat Tan Clarinda Chua Iain Beehuat Tan Lim Kiat Hon Wah Siew Tan Mark Wong Lawrence J K Wee Iain Beehuat Tan Paul Robson Paul Robson Paul Robson | 2017 | 现代生物医学进展2017，17，15： | 37 |
| 3 | Using specific secondary metabolites as markers to evaluate allelopathic potentials of rice varieties and individual plants显示文摘It is a key of the breeding of ailelopathic rice cultivars to evaluate and screen few accessions with ailelopathic traits from numerous rice accessions and individual plants. Rice varieties and individual plants with ailelopathic traits demonstrated their ailelopathic effects by producing and releasing specific secondary metabolites into environment. Therefore, using specific secondary metabolites as markers, the ailelopathic potentials of rice varieties and individual plants could be evaluated by HPLC. The ailelopathic potentials of 3000 rice accessions and some individual plants of F3 and F4 were evaluated by this method in 1 a. If these accessions and plants were evaluated by the traditional method in the field, it would take more than 10 a, furthermore, the breeding process of ailelopathic rice cultivars would be directed and monitored by this method. Isolation and structural identification of specific secondary metabolites was done by LC/MS coupling 1H and 13C NMR. It revealed that allelochemicals | KONG Chuihua XU Xiaohua HU Fei CHEN Xionghui LING Bing TAN Zhongwen | 2002 | Chinese Science Bulletin2002，47，10： | 17 |
| 4 | Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors显示文摘In order to monitor the changes of Microcystis along with temporal and spatial variations, seasonal variation of Microcystis in Lake Taihu was investigated by 16S-23S rRNA internal transcribed spacer denaturing gradient gel electrophoresis (16S-23S rRNA-ITS DGGE) and microscopic evaluation. Samples were collected quarterly at four sites (River Mouth, Meiliang Bay, Cross Area, and Lake Center) from August 2006 to April 2007. Results showed that Microcystis dominated total phytoplankton abundance at the four sites in all seasons except winter. The average annual abundance of Microcystis was relatively high at River Mouth and Meiliang Bay, reaching 81.22 × 106 and 61.32 × 106 cells/L, respectively. For temporal variations, Shannon-Wiener diversity index (H ) according to DGGE profile revealed the richness of Microcystis in summer (H = 1.375 ± 0.034) and winter (H = 1.650 ± 0.032) was lower than that in spring (H = 2.078 ± 0.031) and autumn (H = 2.365 ± 0.032) (P < 0.05). While for spatial variations, the richness of Microcystis at River Mouth (H = 2.015 ± 0.074) was higher than at other sites during four seasons (P < 0.01). Very few dierences of Microcystis diversity in the same season were observed among the other three sites (P > 0.05). Canonical correspondence analysis (CCA) was performed to elucidate the relationships between Microcystis operational taxonomic units (OTUs) composition and the environmental factors. Results of CCA revealed that temperature was strongly positively correlated with the first axis (r = 0.963), while TSS was negative correlated with the second axis (r = -0.716). Phylogenetic tree based on the sequencing results of target bands on DGGE gel indicated that samples collected in summer and winter constituted two separated clusters. | TAN Xiao KONG Fanxiang ZENG Qingfei CAO Huansheng QIAN Shanqin ZHANG Min | 2009 | Journal of Environmental Sciences2009，21，7： | 16 |
| 5 | Preliminary experience with drug-coated balloon angioplasty in primary percutaneous coronary intervention显示文摘We evaluated the clinical feasibility of using drugcoated balloon(DCB) angioplasty in patients undergoingprimary percutaneous coronary intervention(PPCI). Between January 2010 to September 2014,89 STelevation myocardial infarction patients(83% male,mean age 59 ± 14 years) with a total of 89 coronary lesions were treated with DCB during PPCI. Clinical outcomes are reported at 30 d follow-up. Left anterior descending artery was the most common target vessel for PCI(37%). Twenty-eight percent of the patients had underlying diabetes mellitus. Mean left ventricular ejection fraction was 44% ± 11%. DCB-only PCI was the predominant approach(96%) with the remaining 4% of patients receiving bail-out stenting. Thrombolysis in Myocardial Infarction(TIMI) 3 flow was successfully restored in 98% of patients. An average of 1.2 ± 0.5 DCB were used per patient,with mean DCB diameter of 2.6 ± 0.5 mm and average length of 23.2 ± 10.2 mm. At 30-d follow-up,there were 4 deaths(4.5%). No patients experienced abrupt closure of the infarctrelated artery and there was no reported target-lesion failure. Our preliminary experience showed that DCB angioplasty in PPCI was feasible and associated with a high rate of TIMI 3 flow and low 30-d ischaemic event. | Hee Hwa Ho Julian Tan Yau Wei Ooi Kwok Kong Loh Than Htike Aung Nwe Tun Yin Dasdo Antonius Sinaga Fahim Haider Jafary Paul Jau Lueng Ong | 2015 | World Journal of Cardiology2015，7，6： | 14 |
| 6 | Effects of dietary protein restriction on muscle fiber characteristics and m TORC1 pathway in the skeletal muscle of growing-finishing pigs显示文摘Background: To investigate the effects of dietary crude protein(CP) restriction on muscle fiber characteristics and key regulators related to protein deposition in skeletal muscle, a total of 18 growing-finishing pigs(62.30 ± 0.88 kg)were allotted to 3 groups and fed with the recommended adequate protein(AP, 16 % CP) diet, moderately restricted protein(MP, 13 % CP) diet and low protein(LP, 10 % CP) diet, respectively. The skeletal muscle of different locations in pigs, including longissimus dorsi muscle(LDM), psoas major muscle(PMM) and biceps femoris muscle(BFM) were collected and analyzed.Results: Results showed that growing-finishing pigs fed the MP or AP diet improved(P < 0.01) the average daily gain and feed: gain ratio compared with those fed the LP diet, and the MP diet tended to increase(P = 0.09) the weight of LDM. Moreover, the ATP content and energy charge value were varied among muscle samples from different locations of pigs fed the reduced protein diets. We also observed that pigs fed the MP diet up-regulated(P < 0.05) muscular m RNA expression of all the selected key genes, except that myosin heavy chain(My HC) IIb,My HC IIx, while m RNA expression of ubiquitin ligases genes was not affected by dietary CP level. Additionally, the activation of mammalian target of rapamycin complex 1(m TORC1) pathway was stimulated(P < 0.05) in skeletal muscle of the pigs fed the MP or AP diet compared with those fed the LP diet.Conclusion: The results suggest that the pigs fed the MP diet could catch up to the growth performance and the LDM weight of the pigs fed the AP diet, and the underlying mechanism may be partly due to the alteration in energy status, modulation of muscle fiber characteristics and m TORC1 activation as well as its downstream effectors in skeletal muscle of different locations in growing-finishing pigs. | Yinghui Li Fengna Li Li Wu Hongkui Wei Yingying Liu Tiejun Li Bie Tan Xiangfeng Kong Kang Yao Shuai Chen Fei Wu Yehui Duan Yulong Yin | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 13 |
| 7 | Cell-SELEX-based aptamer-conjugated nanomaterials for enhanced targeting of cancer cells显示文摘Early detection and treatment of cancer depends on developing highly sensitive and specific methods for targeting cancer cells. To do this, aptamers, which are generated by a novel technique called cell-SELEX (systematic evolution of ligands by exponential enrichment), have been widely applied in cancer cell targeting based on such merits as high target affinity and specificity, small size, minimal immunogenicity, and ease of chemical modification. Furthermore, aptamers can gain more flexibility as cancer cell targeting tools when conjugated to nanomaterials, including metallic nanoparticles, quantum dots, silica nanoparticles, and carbon nanomaterials, among others. In this review, we discuss the use of cell-SELEX-based aptamer-nanomaterials conjugates as novel molecular tools for enhanced targeting of cancer cells. | KONG RongMei, CHEN Zhuo, YE Mao, ZHANG XiaoBing & TAN WeiHong State Key Laboratory for Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China | 2011 | Science China Chemistry2011，54，8： | 13 |
| 8 | Dyslipidemia and Outcome in Patients with Acute Ischemic Stroke显示文摘Objective To study the relationship between dyslipidemia and outcome in patients with acute ischemic stroke. Methods Data about 1 568 patients with acute ischemic stroke were collected from 4 hospitals in Shandong Province from January 2006 to December 2008. National Institute of Health Stroke Scale(NIHSS) >10 at discharge or death was defined as the outcome. Effect of dyslipidemia on outcome in patients with acute ischemic stroke was analyzed by multivariate logistic regression analysis and propensity score-adjusted analysis, respectively. Results The serum levels of TC, LDL-C, and HDL-C were significantly associated with the outcome in patients with acute ischemic stroke. Multivariate logistic regression analysis and propensity score-adjusted analysis showed that the ORs and 95% CIs were 3.013(1.259, 7.214)/2.655(1.298, 5.43), 3.157(1.306, 7.631)/3.405(1.621, 7.154), and 0.482(0.245, 0.946)/0.51(0.282, 0.921), respectively, for patients with acute ischemic stroke. Hosmer-Lemeshow goodness-of-fit test showed no significant difference in observed and predicted risk in patients with acute ischemic stroke(chi-square=8.235, P=0.411). Conclusion Serum levels of TC, LDL-C, and HDL-C are positively related with the outcome in patients with acute ischemic stroke. | XU Tian ZHANG Jin Tao YANG Mei ZHANG Huan LIU Wen Qing KONG Yan XU Tan ZHANG Yong Hong | 2014 | Biomedical and Environmental Sciences2014，27，2： | 12 |
| 9 | Executive Function Training for Children with Attention Deficit Hyperactivity Disorder显示文摘 | Lan Shuai David Daley Yu-Feng Wang Jin-Song Zhang Yan-Ting Kong Xin Tan Ning Ji | 2017 | Chinese Medical Journal2017，，5： | 10 |
| 10 | An extrapolation approach for aeroengine's transient control law design显示文摘Transient control law ensures that the aeroengine transits to the command operating state rapidly and reliably.Most of the existing approaches for transient control law design have complicated principle and arithmetic.As a result,those approaches are not convenient for application.This paper proposes an extrapolation approach based on the set-point parameters to construct the transient control law,which has a good practicability.In this approach,the transient main fuel control law for acceleration and deceleration process is designed based on the main fuel flow on steady operating state.In order to analyze the designing feature of the extrapolation approach,the simulation results of several different transient control laws designed by the same approach are compared together.The analysis indicates that the aeroengine has a good performance in the transient process and the designing feature of the extrapolation approach conforms to the elements of the turbofan aeroengine. | Kong Xiangxing Wang Xi Tan Daoliang He Ai Liu Yue | 2013 | Chinese Journal of Aeronautics2013，26，5： | 8 |
| 11 | Coral reef-like polyanaline nanotubes prepared by a reactive template of manganese oxide for supercapacitor electrode显示文摘由 nanopaticles 组成的珊瑚像礁石的 PANI nanotubes 被锰氧化物的一个反应模板成功地综合。结构被使用 SEM, TEM,和英尺红外描绘,并且这些 nanotubes 的 supercapacitive 行为与周期的 voltammetry (CV ) 被调查,并且 chargedischarge 分别地测试。533 F/g 的一个最大的特定的电容能在 1 mol/L 被完成有 0.2 ~ 0.8 V 的潜在的范围的水的 H2SO4 (对浸透的甘汞电极) 在为 PANI 电极的一种一半房间安装配置,为电气化学的电容器在电极材料建议它的潜在的申请。 | Ling Ren Wang Fen Ran Yong Tao Tan Lei Zhao Ling Bin Kong Long Kang | 2011 | Chinese Chemical Letters2011，22，8： | 8 |
| 12 | Do major customers promote firms’ innovation?显示文摘This study examines whether and how a concentrated supply chain relationship affects a firm’s innovation decisions. Using data from Chinese listed firms in the manufacturing industry, we find that a concentrated customer base constrains a firm’s R&D investment, where a 1% increase in customer concentration is associated with a 0.011% decrease in R&D investment. To establish causality, we use the instrumental variable method, the reverse causality model, and the Granger causality test to re-examine the relationship and arrive at a consistent conclusion. Results from mechanism analysis suggest that a concentrated customer base constrains the internal fund availability and that the negative relationship between customer concentration and firms’ innovation is less pronounced for firms with more external financial support. Additional analysis reveals that the negative effect of customer concentration mainly affects R&D investment expenditure and that customer concentration also constrains innovation output in China. Overall, our paper reveals the dark side of close customer-supplier relationships and provides new insights into how supply chain relationships affect firms’ innovation decisions. | Jinsong Tan Huijuan Cao Xiangting Kong | 2019 | China Journal of Accounting Research2019，，2： | 8 |
| 13 | Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods显示文摘Background: The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium(Kv) channels in the intestine of piglets during the suckling and post-weaning periods.Results: Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen(PCNA)-positive cells and alkaline phosphatase(AKP) activity, as well as the abundances of E-cadherin,occludin, and Kv1.5 m RNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1to d 21 during the suckling period(P < 0.05). Weaning induced decreases in the percentage of PCNA-positive cells,AKP activity and the abundances of E-cadherin, occludin and zonula occludens(ZO)-1 m RNA or protein in the jejunum on d 1, 3 and 5 post-weaning(P < 0.05). There were lower abundances of E-cadherin, occludin and ZO-1m RNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets(P < 0.05). The abundances of E-cadherin, occludin, ZO-1 and integrin m RNA were positively related to the percentage of PCNA-positive cells.Conclusion: Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels. | Jing Wang Liming Zeng Bie Tan Guangran Li Bo Huang Xia Xiong Fengna Li Xiangfeng Kong Gang Liu Yulong Yin | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 8 |
| 14 | Switching off IMMP2L signaling drives senescence via simultaneous metabolic alteration and blockage of cell death显示文摘 | Lifeng Yuan Linhui Zhai Lili Qian De Huang Yi Ding Handan Xiang Xiaojing Liu J. Will Thompson Juan Liu Yong-Han He Xiao-Qiong Chen Jing Hu Qing-Peng Kong Minjia Tan Xiao-Fan Wang | 2018 | Cell Research2018，28，6： | 7 |
| 15 | Harnessing Vis-NIR broad spectrum for photocatalytic CO2 reduction over carbon quantum dots-decorated ultrathin Bi2WO6 nanosheets显示文摘到充满精力的烃燃料的公司 2 的 photocatalytic 减小是探讨附随地的全球温暖和逼近的精力危机的一个有希望、持续的方法。然而,存在光催化剂的一个广阔多数仅仅能够利用紫外(紫外) 或 / 并且可见的光(力) ,而在红外线附近(NIR ) 区域仍然保持未经勘探。在这研究,碳量点(CQD ) 装饰了 ultrathin 双性人 2 WO 6 nanosheets (UBW ) 被表明是为在 Vis-NIR 宽广光谱上的公司 2 光致还原的有效光催化剂。CQDs/UBW 混合 nanocomposites 的合成过程是高度灵巧的,是引人注目的,包含帮助的一个壶 hexadecyltrimethylammonium 溴化物(CTAB ) 热水的过程。在可见轻照耀下面,优化 1CQDs/UBW (1 个 wt.% CQD 内容) 在太古的双性人 24 生产的显著 9.5 褶层和 3.1 褶层改进 > WO 6 nanoplatelets (PBW ) 和赤裸的 UBW 分别地。更重要地, CQDs/UBW 的 photocatalytic 应答成功地被扩大到 NIR 区域,没有包含任何稀土元素或高贵金属,它被完成。减小能被归因于 synergistic 的 驾驶NIR 的公司2的实现(i)完成 ultrathin nanostructures 并且高度暴露了{ 001 } UBW 的活跃方面,( ii ) UBW 和 CQD 的优秀光谱的联合,在 UBW 能由 CQD 的起来变换的光致发光是激动的的地方,并且( iii ) CQD 到的撤退电子的性质套住 photogenerated 电子并且延迟充电搬运人的再结合。 | Xin Ying Kong Wen Liang Tan Boon-Junn Ng Siang-Piao Chai Abdul Rahman Mohamed | 2017 | Nano Research2017，10，5： | 7 |
| 16 | Complement C7 is a novel risk gene for Alzheimer's disease in Han Chinese显示文摘Alzheimer's disease is the most common neurodegenerative disease,and has a high level of genetic heritability and population heterogeneity.In this study,we performed the whole-exome sequencing of Han Chinese patients with familial and/or early-onset Alzheimer's disease,followed by independent validation,imaging analysis and function characterization.We identified an exome-wide significant rare missense variant rs3792646(p.K420Q)in the C7 gene in the discovery stage(P=1.09×10^(-6),odds ratio=7.853)and confirmed the association in different cohorts and a combined sample(1615 cases and 2832 controls,P_(combined)=2.99×10^(-7),odds ratio=1.930).The risk allele was associated with decreased hippocampal volume and poorer working memory performance in early adulthood,thus resulting in an earlier age of disease onset.Overexpression of the mutant p.K420Q disturbed cell viability,immune activation and β-amyloid processing.Electrophysiological analyses showed that the mutant p.K420Q impairs the inhibitory effect of wild type C7 on the excitatory synaptic transmission in pyramidal neurons.These findings suggested that C7 is a novel risk gene for Alzheimer’s disease in Han Chinese. | Deng-Feng Zhang Yu Fan Min Xu Guihong Wang Dong Wang Jin Li Li-Li Kong Hejiang Zhou Rongcan Luo Rui Bi Yong Wu Guo-Dong Li Ming Li Xiong-Jian Luo Hong-Yan Jiang Liwen Tan Chunjiu Zhong Yiru Fang Chen Zhang Nengyin Sheng Tianzi Jiang Yong-Gang Yao | 2019 | National Science Review2019，6，2： | 6 |
| 17 | Tianwen-1 MINPA observations in the solar wind显示文摘The Mars Ion and Neutral Particle Analyzer(MINPA)is one of the three scientific instruments onboard the Tianwen-1 orbiter to investigate the Martian space environment.During Tianwen-1’s transfer orbit to Mars,the MINPA was switched on to measure the solar wind ions.Here,we present the first results of the MINPA observations in the solar wind.During cruise,nearly half of the MINPA ion field-of-view(FOV)was blocked by the lander capsule;thus only the solar-wind ions with azimuthal speeds pointing towards the unblocked FOV sectors could be detected.We perform a detailed comparison of the MINPA’s solar wind observations with data from Earth-based missions when MINPA reached its count-rate peak,finding a general consistency of the ion moments between them.The blocking effect due to the lander is evaluated quantitatively under varying solar-wind velocity conditions.Despite the blocking effect,the MINPA’s solar wind measurements during the transfer orbit suggest a good performance. | AiBing Zhang LingGao Kong WenYa Li Lei Li BinBin Tang ZhaoJin Rong Yong Wei JiJie Ma YiTeng Zhang LiangHai Xie YuXian Wang JianSen He Bin Liu WenJing Wang Bin Su JiaWei Li Xu Tan Fang Wang TaiFeng Jin FuHao Qiao Peter Wurz Yan Zhu YunFei Bai YiRen Li XinBo Zhu YueQiang Sun YongLiao Zou Chi Wang | 2022 | Earth and Planetary Physics2022，6，1： | 5 |
| 18 | Face Masks in the New COVID-19 Normal: Materials, Testing,and Perspectives显示文摘The increasing prevalence of infectious diseases in recent decades has posed a serious threat to public health.Routes of transmission differ,but the respiratory droplet or airborne route has the greatest potential to disrupt social intercourse,while being amenable to prevention by the humble facemask.Different types of masks give different levels of protection to the user.The ongoing COVID-19 pandemic has even resulted in a global shortage of face masks and the raw materials that go into them,driving individuals to self-produce masks from household items.At the same time,research has been accelerated towards improving the quality and performance of face masks,e.g.,by introducing properties such as antimicrobial activity and superhydrophobicity.This review will cover mask-wearing from the public health perspective,the technical details of commercial and home-made masks,and recent advances in mask engineering,disinfection,and materials and discuss the sustainability of mask-wearing and mask production into the future. | Ming Hui Chua Weiren Cheng Shermin Simin Goh Junhua Kong Bing Li Jason Y.CLim Lu Mao Suxi Wang Kun Xue Le Yang Enyi Ye Kangyi Zhang Wun Chet Davy Cheong Beng Hoon Tan Zibiao Li Ban Hock Tan Xian Jun Loh | 2020 | Research2020，，1： | 5 |
| 19 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 20 | Locomotor activity and anxiety status, but not spatial working memory,are affected in mice after brief exposure to cuprizone显示文摘Chronic long-term exposure to cuprizone causes severe brain demyelination in mice,which leads to changes in locomotion,working memory and anxiety.These findings suggest the importance of intact myelin for these behaviors.This study aimed to investigate the possible behavioral changes in mice with mild oligodendrocyte/myelin damage that parallels the white matter changes seen in the brains of patients with psychiatric disporders.We used the cuprizonetreated mouse model to test both tissue changes and behavioral functions(locomotor activity,anxiety status,and spatial working memory).The results showed that mice given cuprizone in their diet for 7 days had no significant myelin breakdown as evaluated by immunohistochemical staining for myelin basic protein,while the number of mature oligodendrocytes was reduced.The number and length of Caspr protein clusters,a structural marker of the node of Ranvier,did not change.The locomotor activity of the cuprizonetreated mice increased whereas their anxiety levels were lower than in normal controls;spatial working memory,however,did not change.These results,for the first time,link emotion-related behavior with mild white matter damage in cuprizone-treated mice. | Handi Zhang Yanbo Zhang Haiyun Xu Lingyan Wang Jinsong Zhao Junhui Wang Zhijun Zhang Qingrong Tan Jiming Kong Qingjun Huang Xin-Min Li | 2013 | Neuroscience Bulletin2013，29，5： | 4 |
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