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| 1 | Differential gene expression in nontransgenic and transgenic“M.26”apple overexpressing a peach CBF gene during the transition from eco-dormancy to bud break显示文摘The CBF signal pathway is responsible for a significant portion of plant responses to low temperature and freezing.Overexpression of CBF genes in model organisms such as Arabidopsis thaliana enhances abiotic stress tolerance but also reduces growth.In addition to these effects,overexpression of the peach(Prunus persica[L.]Batsch)CBF1 gene in transgenic apple(Malus x domestica Borkh.)line T166 also results in early entry into and late exit from dormancy.Although the regulation of dormancy-induction and dormancy-release occur while the CBF regulon is operative in perennial,woody plants,how overexpression of CBF1 affects these dormancy-related changes in gene expression is incompletely understood.The objective of the present study was to characterize global changes in gene expression in peach CBF1-overexpressing and non-transformed apple bark tissues at different states of dormancy via RNA-seq.RNAseq bioinformatics data was confirmed by RT-qPCR on a number of genes.Results indicate that the greatest number of significantly differentially expressed genes(DEGs)occurred in April when dormancy release and bud break normally occur but are delayed in Line T166.Genes involved in storage and inactivation of auxin,GA,and cytokinin were generally upregulated in T166 in April,while those for biosynthesis,uptake or signal transduction were generally downregulated in T166.Genes for cell division and cambial growth were also downregulated in T166 relative to the non-transformed line.These data suggest that overexpression of the peach CBF1 gene impacts growth hormone homeostasis and as a result the activation of growth in the spring,and most likely growth cessation in the fall as well. | Timothy Artlip Adam McDermaid Qin Ma Michael Wisniewski | 2019 | Horticulture Research2019，6，1： | 6 |
| 2 | Utility of MtCOI polymerase chain reaction-restriction fragment length polymorphism in differentiating between Q and B whitefly Bemisia tabaci biotypes显示文摘侵略,杀虫药剂抵抗, Q 白苍蝇遗传因子型,逐渐地经由植物材料的调停人的运动包括 US 传播了到另外的国家。我们估计了基于 VspI 的 mtCOI 的用途(线粒体细胞色素 oxidase 我) 是的聚合酶链反应限制碎片长度多型性(PCR-RFLP ) 技术为在亚利桑那把 Q 区分开来与主导的 B 遗传因子型的一种快速、划算、可靠的选择。用定序的标准 mtCOI 基因和 mtCOI PCR-RFLP 技术,我们八个白苍蝇五个个人拉紧的 biotyped 各在亚利桑那在不同地点从一品红和棉花收集了。完全的词语索引在二个方法之间被观察,与作为象 B 遗传因子型的 Q 遗传因子型和五件样品被识别的三紧张。我们也在在 GenBank 数据库当前可得到的 B 和 Q 遗传因子型白人苍蝇为 VspI 多型性扫描了 mtCOI 基因序列。这全球屏蔽分别地为 Q 和 B 遗传因子型揭示了三和四种 VspI 多态的类型的存在。不过,所有三个 VspI 多态的 Q 遗传因子型白人苍蝇分享了能被用来把 Q 遗传因子型区分开来与识别的四个 VspI 多态的 B 遗传因子型白人苍蝇的一个普通、唯一的 VspI 地点。这些结果证明基于 VspI 的 mtCOI 基因 PCR-RFLP 为区分白色在 US 并且在其它地方飞的 Q 和 B 遗传因子型提供一个可靠诊断工具。 | Wei-Hua Ma Xian-Chun Li Timothy J. Dennehy Chao-Liang Lei Mo Wang Benjamin A. Degain Robert L. Nichols | 2009 | Insect Science2009，16，2： | 5 |
| 3 | Antiviral effect of interferon lambda against West Nile virus显示文摘 | Dongling Ma Dong Jiang Min Qing Jessica M. Weidner Xiaowang Qu Haitao Guo Jinhong Chang Baohua Gu Pei-Yong Shi Timothy M. Block Ju-Tao Guo | 2009 | Antiviral Research2009，，1： | 1 |
| 4 | Accuracy of invasive and noninvasive tests to diagnose Helicobacter pylori infection显示文摘 | Alan F. Cutler Suzanne Havstad Chen K. Ma Martin J. Blaser Guillermo I. Perez-Perez Timothy T. Schubert | 1995 | Gastroenterology1995，，1： | 1 |
| 5 | Tranexamic acid for intracerebral haemorrhage within 2 hours of onset: protocol of a phase Ⅱ randomised placebo-controlled double-blind multicentre trial显示文摘Rationale Haematoma growth is common early after intracerebral haemorrhage(ICH),and is a key determinant of outcome.Tranexamic acid,a widely available antifibrinolytic agent with an excellent safety profile,may reduce haematoma growth.Methods and design Stopping intracerebral haemorrhage with tranexamic acid for hyperacute onset presentation including mobile stroke units(STOP-MSU)is a phase Ⅱ double-blind,randomised,placebo-controlled,multicentre,international investigator-led clinical trial,conducted within the estimand statistical framework.Hypothesis In patients with spontaneous ICH,treatment with tranexamic acid within 2 hours of onset will reduce haematoma expansion compared with placebo.Sample size estimates A sample size of 180 patients(90 in each arm)would be required to detect an absolute difference in the primary outcome of 20%(placebo 39%vs treatment 19%)under a two-tailed significance level of 0.05.An adaptive sample size re-estimation based on the outcomes of 144 patients will allow a possible increase to a prespecified maximum of 326 patients.Intervention Participants will receive 1 g intravenous tranexamic acid over 10 min,followed by 1 g intravenous tranexamic acid over 8 hours;or matching placebo.Primary efficacy measure The primary efficacy measure is the proportion of patients with haematoma growth by 24±6 hours,defined as either≥33%relative increase or≥6 mL absolute increase in haematoma volume between baseline and follow-up CT scan.Discussion We describe the rationale and protocol of STOP-MSU,a phase Ⅱ trial of tranexamic acid in patients with ICH within 2 hours from onset,based in participating mobile stroke units and emergency departments. | Nawaf Yassi Henry Zhao Leonid Churilov Bruce C V Campbell Teddy Wu Henry Ma Andrew Cheung Timothy Kleinig Helen Brown Philip Choi Jiann-Shing Jeng Annemarei Ranta Hao-Kuang Wang Geoffrey C Cloud Rohan Grimley Darshan Shah Neil Spratt Der-Yang Cho Karim Mahawish Lauren Sanders John Worthington Ben Clissold Atte Meretoja Vignan Yogendrakumar Mai Duy Ton Duc Phuc Dang Nguyen Thai My Phuong Huy-Thang Nguyen Chung Y Hsu Gagan Sharma Peter J Mitchell Bernard Yan Mark W Parsons Christopher Levi Geoffrey A Donnan Stephen M Davis | 2022 | Stroke & Vascular Neurology2022，7，2： | 1 |
| 6 | A phase II study of UCN-01 in combination with irinotecan in patients with metastatic triple negative breast cancer显示文摘 | Cynthia X. Ma Matthew J. C. Ellis Gina R. Petroni Zhanfang Guo Shi-rong Cai Christine E. Ryan A. Craig Lockhart Michael J. Naughton Timothy J. Pluard Christiana M. Brenin Joel Picus Allison N. Creekmore Tibu Mwandoro Erin R. Yarde Jerry Reed Mark Ebbert P | 2013 | Breast Cancer Research and Treatment2013，，2： | 1 |
| 7 | Evaluation of the association of C5 with neovascular age-related macular degeneration and polypoidal choroidal vasculopathy显示文摘Background:Neovascular age-related macular degeneration(AMD)and polypoidal choroidal vasculopathy(PCV)are sight-threatening maculopathies with both environmental and genetic risk factors.We have previously shown relative risks posed by genes of the complement pathways to neovascular AMD and PCV.Methods:In this study,we investigated the haplotype-tagging single nucleotide polymorphisms(SNPs)in the complement component 5(C5)gene in 708 unrelated Chinese individuals:200 neovascular AMD patients,233 PCV patients and 275 controls.Six tagging SNPs in C5 were genotyped.Univariate single SNP association analysis,haplotype-based association analysis and gene-gene interaction analysis between C5 and other AMD-associated genes were performed.Results:The results revealed none of the six tagging SNPs of the C5 gene had a significant association with neovascular AMD or PCV(P>0.05).We also found insignificant haplotype-based association,and no significant SNPSNP interaction between C5 and other genes(including C2-CFB-RDBP-SKIV2L,SERPING1,CETP,ABCG1,PGF,ANGPT2,CFH and HTRA1)for neovascular AMD and PCV.Conclusions:This study showed no statistical significance in the genetic association of C5 with neovascular AMD or PCV in a Hong Kong Chinese population.Further studies in large samples from different populations are warranted to elucidate the role of C5 in the genetic susceptibility of AMD and PCV. | Ke Liu Li Ma Timothy Y.Y.Lai Marten E.Brelen Pancy O.S.Tam Clement C.Tham Chi Pui Pang Li Jia Chen | 2019 | Eye and Vision2019，6，1： | 1 |
| 8 | Finger clubbing and a lung mass 显示文摘 | John DU Timothy MA Brian PW | 1999 | Chest1999，115，6： | 1 |
| 9 | Friction-based riveting technique for AZ31 magnesium alloy显示文摘A new friction-based riveting technique, Rotating Hammer Riveting(RHR), is demonstrated to fully form AZ31 Mg rivet heads in a mere 0.23 s. Heat and pressure generated through severe plastic deformation during the process was sufficient to form the Mg rivet head without the need for a pre-heating operation. Due to preliminary twinning and followed by dynamic recrystallization, AZ31 Mg grains in the rivet head were refined during RHR, which enhance the formability of Mg rivets by triggering grain boundary sliding and reducing plastic anisotropy of Mg. In addition, RHR joints showed a metallurgical bond between the rivet head and top AZ31 Mg sheet, which eliminates a significant pathway for corrosion. | Tianhao Wang Scott Whalen Xiaolong Ma Joshua Silverstein Hrishikesh Das Madhusudhan R.Pallaka Angel Ortiz Timothy Roosendaal Piyush Upadhyay Keerti S Kappagantula | 2022 | Journal of Magnesium and Alloys2022，10，1： | 1 |
| 10 | Teleoperation of a Robot Manipulator Using a Vision Based Human Robot Interface 显示文摘 | Jonathan Kofman Xianghai Wu Timothy J Luu Siddharth Ver- ma | 2005 | IEEE Transactions On Industrial E- lectronics2005，52，5： | 1 |
| 11 | The Chemokine Receptor CXCR4 Is Required for the Retention of B Lineage and Granulocytic Precursors within the Bone Marrow Microenvironment显示文摘 | Qing Ma Dan Jones Timothy A Springer | 1999 | Immunity1999，，4： | 1 |
| 12 | Use of basis images for detection and classification of celiac disease显示文摘 | Feng Liu Ying Wang Timothy A. Burkhart Manuel Francisco Gonzá lez Penedo Shao-dong Ma Edward J. Ciaccio Christina A. Tennyson Govind Bhagat Suzanne K. Lewis Peter H. Green | 2014 | Bio-Medical Materials and Engineering2014，，6： | 1 |
| 13 | The Chemokine Receptor CXCR4 Is Required for the Retention of B Lineage and Granulocytic Precursors within the Bone Marrow Microenvironment显示文摘 | Qing Ma Dan Jones Timothy A Springer | 1999 | Immunity1999，，4： | 1 |
| 14 | Roles of a metastasis-associated molecule, RTVP-1, in cancer immunosurveillance显示文摘Objective: To elucidate distinct functions of a recently identified cancer metastasis-associated molecule, related to testes-specific, vespid, and pathogenesis protein-1 (RTVP-1) in the mammalian immune system. Methods: Immunohistochemical assays and functional analysis on the immune system were performed on RTVP-/- mice and RTVP-1+/+ mice. Protein-protein interaction (PPI) was used to predict the functions of RTVP-1. Results: Abnormal lymphocyte growth kinetics and reduced numbers of CD8+ T cells were revealed in lymph nodes of RTVP-1-/- mice. Expression of phenotypic markers of maturation in bone marrow-derived dendritic cells (DC) was impaired in RTVP-1-/- DC following antigenic stimulation in vitro and RTVP-1-/- DC failed to provide normal CD4+ T cell stimulatory activities in vivo. RTVP-1-/- mice failed to generate normal CTL or antibody responses in vivo after vaccination. In vivo tumor challenge experiments using a mouse cancer cell line demonstrated that the growth rate of subcutaneous tumors in RTVP-1-/- mice was significantly increased and CD8+ T cell infiltration significantly reduced compared to RTVP-1+/+ mice. PPI showed that RTVP-1 protein closely interacted with molecules associated with immune response and cancer metastasis. Conclusion: RTVP-1 might function as a tumor metastasis suppressor and immunosurveillance molecule in cancer. | Sun Junwen Chang Wenjun Yang Guang Hou Jianguo Ma Liye Zhang Hongwei Tan Xiaojie He Xiang Timothy C. Thompson Cao Guangwen | 2008 | Journal of Medical Colleges of PLA(China)2008，23，4： | 0 |
| 15 | Nanoengineering Metal–Organic Frameworks and Derivatives for Electrosynthesis of Ammonia显示文摘Electrocatalytic synthesis under mild conditions has become increasingly important as one of the practical alternatives for industrial applications,especially for the green ammonia(NH_(3))industry.A properly engineered electrocatalyst plays a vital role in the realization of superior catalytic performance.Among various types of promising nanomaterials,metal–organic frameworks(MOFs)are competitive candidates for developing efficient electrocatalytic NH_(3) synthesis from simple nitrogen-containing molecules or ions,such as N_(2) and NO_(3)^(−).In this review,recent advances in the development of electrocatalysts derived from MOFs for the electrosynthesis of NH_(3) are collected,categorized,and discussed,including their application in the N_(2) reduction reaction(NRR)and the NO_(3)^(−)reduction reaction(NO3RR).Firstly,the fundamental principles are illustrated,such as plausible mechanisms of NH_(3) generation from N_(2) and NO_(3)^(−),the apparatus of corresponding electrocatalysis,parameters for evaluation of reaction efficiency,and detection methods of yielding NH_(3).Then,the electrocatalysts for NRR processes are discussed in detail,including pristine MOFs,MOF-hybrids,MOF-derived N-doped porous carbons,single atomic catalysts from pyrolysis of MOFs,and other MOF-related materials.Subsequently,MOF-related NO3RR processes are also listed and discussed.Finally,the existing challenges and prospects for the rational design and fabrication of electrocatalysts from MOFs for electrochemical NH_(3) synthesis are presented,such as the evolution of investigation methods with artificial intelligence,innovation in synthetic methods of MOF-related catalysts,advancement of characterization techniques,and extended electrocatalytic reactions. | Daming Feng Lixue Zhou Timothy J.White Anthony K.Cheetham Tianyi Ma Fengxia Wei | 2023 | Nano-Micro Letters2023，15，11： | 0 |
| 16 | Clinical efficacy and safety of mesenchymal stem cells in patients with diabetes mellitus显示文摘Diabetes mellitus(DM)as a chronic metabolic disease is threatening human health seriously.The aim of this study was to systematically review the evidence from RCTs and conduct comprehensive assessment of therapeutic efficacy and safety of mesenchymal stem cells(MSCs)in DM.PubMed,Web of Science,Ovid,the Cochrane Library and CNKI were searched until April 30,2020.Seven RCTs were eligible,including 413 participants. | He Jingjing Kong Desheng Asiamah Ernest Am ponsah Guo Ruiyun Feng Baofeng Ma Jun Timothy O'Brien Cui Huixian | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 17 | Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains显示文摘Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing animals.At present,the impact of livestock-associated CREC strains on human populations at the national level is unknown.Here,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence.We also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin.Overall,631/29799(2.1%)clinical Escherichia coli(E.coli)isolates were identified as CREC.Multivariable analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC infection.In general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),respectively.Among all the Chinese NDM-positive E.coli(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels.All the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe. | Yingbo Shen Fupin Hu Yongqiang Wang Dandan Yin Lu Yang Yiqiang Chen Chunyan Xu Jiyun Li Junyao Jiang Xueyang Wang Yulin Fu Dongyan Shao Dejun Liu Tengfei Ma Chang Cai Zhangqi Shen Shaolin Wang Juan Li Rong Zhang Yuebin Ke Congming Wu Jianzhong Shen Timothy R.Walsh Yang Wang | 2022 | Engineering2022，8，8： | 0 |
| 18 | A unique hyperdynamic dimer interface permits small molecule perturbation of the melanoma oncoprotein MITF for melanoma therapy显示文摘Microphthalmia transcription factor(MITF)regulates melanocyte development and is the“lineage-specific survival”oncogene of melanoma.MITF is essential for melanoma initiation,progression,and relapse and has been considered an important therapeutic target;however,direct inhibition of MITF through small molecules is considered impossible,due to the absence of a ligand-binding pocket for drug design.Here,our structural analyses show that the structure of MITF is hyperdynamic because of its out-of-register leucine zipper with a 3-residue insertion.The dynamic MITF is highly vulnerable to dimer-disrupting mutations,as we observed that MITF loss-of-function mutations in human Waardenburg syndrome type 2 A are frequently located on the dimer interface and disrupt the dimer forming ability accordingly.These observations suggest a unique opportunity to inhibit MITF with small molecules capable of disrupting the MITF dimer.From a high throughput screening against 654,650 compounds,we discovered compound TT-012,which specifically binds to dynamic MITF and destroys the latter’s dimer formation and DNA-binding ability.Using chromatin immunoprecipitation assay and RNA sequencing,we showed that TT-012 inhibits the transcriptional activity of MITF in B16F10 melanoma cells.In addition,TT-012 inhibits the growth of high-MITF melanoma cells,and inhibits the tumor growth and metastasis with tolerable toxicity to liver and immune cells in animal models.Together,this study demonstrates a unique hyperdynamic dimer interface in melanoma oncoprotein MITF,and reveals a novel approach to therapeutically suppress MITF activity. | Zaizhou Liu Kaige Chen Jun Dai Peng Xu Wei Sun Wanlin Liu Zhixin Zhao Steven P.Bennett Peifeng Li Tiancheng Ma Yuqi Lin Akinori Kawakami Jing Yu Fei Wang Chunxi Wang Miao Li Peter Chase Peter Hodder Timothy P.Spicer Louis Scampavia Chunyang Cao Lifeng Pan Jiajia Dong Yong Chen Biao Yu Min Guo Pengfei Fang David E.Fisher Jing Wang | 2023 | Cell Research2023，33，1： | 0 |
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