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| 1 | Effect of extrusion speed on microstructure and mechanical properties of the Mg-Ca binary alloy显示文摘This work reported the effect of extrusion speeds on the microstructures and mechanical properties of Mg-Ca binary alloy.The results showed that yield strength of the as-extruded Mg-1.2wt.%Ca alloys decrease from∼360MPa to∼258MPa as the ram speed increases from 0.4mm/s to 2.4 mm/s,and the elongation increases from∼3.9%to∼12.2%.The microstructure changes from bimodal grain feature to the complete dynamical recrystallization(DRX)with increase of the extrusion speed.The ultrafine DRXed grains in size of∼0.85μm,the numerous nano-Mg_(2)Ca particles dispersing along the grain boundaries and interiors,as well as the high density of residual dislocations,should account for the high strength.It is believed that the high degree of dynamic recrystallization and the resulting texture randomization play the critical roles in the ductility enhancement of the high-speed extruded Mg alloys. | Jingren Li Aiyue Zhang Hucheng Pan Yuping Ren Zhuoran Zeng Qiuyan Huang Changlin Yang Lifeng Ma Gaowu Qin | 2021 | Journal of Magnesium and Alloys2021，9，4： | 9 |
| 2 | Expression of Beclin1 in non small cell lung cancer and its clinical significance显示文摘Objective:To investigate the expression of autophagy related gene;Beclin1 in human non-small cell lung cancer(NSCLC)tissues.Methods:Protein expression of Beclin1 was determined by immunofluorescence staining and Western Blot,mRNA expression was analyzed by RT-PCR.Results:Immunofluorescence staining revealed that the level of Beclin1 expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%,P=0.000).The Beclin1 mRNA expression in lung cancer,adjacent noncancerous tissues and normal tissues was 1.372(±0.475)1.721(±0.521)and 1.553(±0.554)when F = 15.0,P < 0.01.Beclin1 protein expression in lung cancer,adjacent noncancerous tissues and normal tissues was 3.453(±0.852)5.423(±1.351)and 6.878(±0.997)F = 11.2,P < 0.01.Conclusion:The protein and mRNA expression of Beclin1 in lung cancer was much lower than those in and around cancer tissues and normal tissues,those differences having statistical significance.However in adjacent noncancerous tissues and normal tissues,the expression showed no difference. | Quan Liu Jianjun Wang Yongcheng Pan Lifeng Meng Xi Zhan | 2008 | Journal of Nanjing Medical University2008，22，2： | 6 |
| 3 | The Genomes of the Allohexaploid Echinochloa crus-galli and Its Progenitors Provide Insights into Polyploidization-Driven Adaptation显示文摘The hexaploid species Echinochloa crus-galli is one of the most detrimental weeds in crop fields,especially in rice paddies.Its evolutionary history is similar to that of bread wheat,arising through polyploidization after hybridization between a tetraploid and a diploid species.In this study,we generated and analyzed high-quality genome sequences of diploid(E.haploclada),tetraploid(E.oryzicola),and hexaploid(E.crus-galli)Echinochloa species.Gene family analysis showed a significant loss of disease-resistance genes such as those encoding NB-ARC domain-containing proteins during Echinochloa polyploidization,contrary to their significant expansionduring wheat polyploidization,suggesting that natural selection might favor reduced investment in resistance in this weed to maximize its growth and reproduction.In contrast to the asymmetric patterns of genome evolution observed in wheat and other crops,no significant differences in selection pressure were detected between the subgenomes in E.oryzicola and E.crus-galli.In addition,distinctive differences in subgenome transcriptome dynamics during hexaploidization were observed between E.crus-galli and bread wheat.Collectively,our study documents genomic mechanisms underlying the adaptation of a major agricultural weed during polyploidization.The genomic and transcriptomic resources of three Echinochloa species and new insights into the polyploidization-driven adaptive evolution would be useful for future breeding cereal crops. | Chu-Yu Ye Dongya Wu Lingfeng Mao Lei Jia Jie Qiu Sangting Lao Meihong Chen Bowen Jiang Wei Tang Qiong Peng Lang Pan Lifeng Wang Xiaoxiao Feng Longbiao Guo Chulong Zhang Elizabeth A.Kellogg Kenneth M.Olsen Lianyang Bai Longjiang Fan | 2020 | Molecular Plant2020，13，9： | 4 |
| 4 | Combination of NMR spectroscopy and X-ray crystallography offers unique advantages for elucidation of the structural basis of protein complex assembly显示文摘NMR spectroscopy and X-ray crystallography are two premium methods for determining the atomic structures of macro-biomolecular complexes.Each method has unique strengths and weaknesses.While the two techniques are highly complementary,they have generally been used separately to address the structure and functions of biomolecular complexes.In this review,we emphasize that the combination of NMR spectroscopy and X-ray crystallography offers unique power for elucidating the structures of complicated protein assemblies.We demonstrate,using several recent examples from our own laboratory,that the exquisite sensitivity of NMR spectroscopy in detecting the conformational properties of individual atoms in proteins and their complexes,without any prior knowledge of conformation,is highly valuable for obtaining the high quality crystals necessary for structure determination by X-ray crystallography.Thus NMR spectroscopy,in addition to answering many unique structural biology questions that can be addressed specifically by that technique,can be exceedingly powerful in modern structural biology when combined with other techniques including X-ray crystallography and cryo-electron microscopy. | FENG Wei PAN LiFeng ZHANG MingJie | 2011 | Science China(Life Sciences)2011，54，2： | 3 |
| 5 | Wheat MADS-box gene TaSEP3-D1 negatively regulates heading date显示文摘The MADS-box gene plays an important role in regulating plant growth and development.In this study,a SEP3-like MADS-box gene TaSEP3-1 was isolated from bread wheat.The expression patterns of the three homoeologs TaSEP3-A1,TaSEP3-B1,and TaSEP3-D1 were similar,and higher expression levels were detected in floral organs and developing kernels.TaSEP3-D1 was located in the nucleus and cytoplasm and possessed transactivation activity in yeast.Homoeolog sequence polymorphism analysis identified four,three,and four haplotypes of TaSEP3-A1,TaSEP3-B1,and TaSEP3-D1,respectively,and the haplotypes of TaSEP3-D1 had larger effects on agronomic traits than those of TaSEP3-A1 and TaSEP3-B1.D1_h4,significantly associated with heading date,plant height,and other yield-related traits,was the favored haplotype of TaSEP3-D1.Transgenic wheat genotypes overexpressing TaSEP3-D1 exhibited delayed heading and reduced plant height,indicating a role in regulating heading date and plant development.These results shed light on the role of TaSEP3-D1 in wheat plant development.The favored haplotype of TaSEP3-D1 can be applied in breeding to improve plant architecture and yield in wheat. | Lei Zhang Hao Zhang Linyi Qiao Lingfeng Miao Dong Yan Pan Liu Guangyao Zhao Jizeng Jia Lifeng Gao | 2021 | The Crop Journal2021，9，5： | 2 |
| 6 | Estrogen upregulates DNA2 expression through the PI3K-AKT pathway in endometrial carcinoma显示文摘Endometrial cancer is the most common gynecological malignancy,affecting up to 3%of women at some point during their lifetime(Morice et al.,2016;Li and Wang,2021).Based on the pathogenesis and bio‑logical behavioral characteristics,endometrial cancer can be divided into estrogen-dependent(I)and non-estrogen-dependent(II)types(Ulrich,2011).Type I accounts for approximately 80%of cases,of which the majority are endometrioid carcinomas,and the remaining are mucinous adenocarcinomas(Setiawan et al.,2013). | Xinyan WANG Xiuling XU Ting ZHANG Yang JIN Sheng XU Lifeng CHEN Yucheng LAI Ling ZHANG Ruolang PAN Yan YU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，3： | 2 |
| 7 | Improved strength and ductility of AZ31B Mg alloy sheets processed by accumulated extrusion bonding with artificial cooling显示文摘In this study,accumulated extrusion bonding(AEB)process with application of artificial water cooling was successfully performed to fabricate fined-grains AZ31B Mg alloy sheets at 150℃,200℃ and 250℃.The resultant microstructure and mechanical properties are systematically investigated.It reveals that the processing temperature has an important effect on the microstructural evolution during extrusion.During AEB process at 150℃ and 200℃,{10-12}tensile twinning was activated at early stage of extrusion,and subsequently continuous dynamic recrystallization(CDRX)occurred and dominated the further deformation.However,for the sample extruded at 250℃,hardly any twins can be observed,and new fined dynamic recrystallized grains were found along grain boundaries.Artificial cooling was utilized to reduce the rate of grain growth out of the extrusion die,resulting the grains significantly refined from 11μm to 2.5μm.Local high dislocation density region was also observed in the microstructure of sample processed at 150℃ in artificial cooling condition,and the degree decreased with the processing temperature increase.The results summarized from tensile tests indicated that due to the grain refinement the strength and ductility was significantly enhanced(YS of 186 MPa vs.145 MPa,UTS of 391 MPa vs.336 MPa and FE of 31.5%vs.24.5%compared with the as-received sample).Subsequently,annealing treatment at different temperatures was applied to eliminate the high dislocation density.The sample annealed at 200℃ exhibited the best comprehensive mechanical property with YS of 179 MPa,UTS of 390 MPa and FE of 33.0%.As the annealing temperature increasing,the dislocation density was reduced by static recrystallization(SRX)and grain growth,leading to a decreased strength and ductility. | Tingzhuang Han Jingfeng Zou Guangsheng Huang Lifeng Ma Chaojie Che Weitao Jia Lifei Wang Fusheng Pan | 2021 | Journal of Magnesium and Alloys2021，9，5： | 2 |
| 8 | Statistical Analysis of Thunderstorms on the Eastern Tibetan Plateau Based on Modified Thunderstorm Indices显示文摘The Tibetan Plateau, with an average altitude above 4000 m, is the highest and largest plateau in the world. The frequency of thunderstorms in this region is extremely high. Many indices are used in operational forecasting to assess the stability of the atmosphere and predict the probability of severe thunderstorm development. One of the disadvantages of many of these indices is that they are mainly based on observations from plains. However, considering the Plateau's high elevation, most convective parameters cannot be applied directly, or their application is ineffective. The pre-convective environment on thunderstorm days in this region is investigated based on sounding data obtained throughout a five-year period(2006–10).Thunderstorms occur over the Tibetan Plateau under conditions that differ strikingly from those in plains. On this basis,stability indices, such as the Showalter index(including SI and SICCL), and the K index are improved to better assess the thunderstorm environments on the Plateau. Verification parameters, such as the true-skill statistic(TSS) and Heidke skill score(HSS), are adopted to evaluate the optimal thresholds and relative forecast skill for each modified index. Lastly, the modified indices are verified with a two-year independent dataset(2011–12), showing satisfactory results for the modified indices. For determining whether or not a thunderstorm day is likely to occur, we recommend the modified SICCLindex. | YOU Wei ZANG Zengliang PAN Xiaobin ZHANG Lifeng LI Yi | 2015 | Advances in Atmospheric Sciences2015，32，4： | 2 |
| 9 | Single-machine-based production scheduling model integrated preventive maintenance planning显示文摘 | PAN Ershun LIAO Wenzhu XI Lifeng | 2010 | The International Journal of Advanced Manufacturing Technology2010，50，14： | 1 |
| 10 | Extensions of PDZ domains as important structural and functional elements显示文摘‘Divide and conquer’has been the guiding strategy for the study of protein structure and function.Proteins are divided into domains with each domain having a canonical structural definition depending on its type.In this review,we push forward with the interesting observation that many domains have regions outside of their canonical definition that affect their structure and function;we call these regions‘extensions’.We focus on the highly abundant PDZ(PSD-95,DLG1 and ZO-1)domain.Using bioinformatics,we find that many PDZ domains have potential extensions and we developed an openly-accessible website to display our results(http://gffzzef4e62913434470fhovcwuonu6vuo6c0v.ffgz.tsg.suse.edu.cn/pdzex/).We propose,using well-studied PDZ domains as illustrative examples,that the roles of PDZ extensions can be classified into at least four categories:1)protein dynamics-based modulation of target binding affinity,2)provision of binding sites for macro-molecular assembly,3)structural integration of multi-domain modules,and 4)expansion of the target ligand-binding pocket.Our review highlights the potential structural and functional importance of domain extensions,highlighting the significance of looking beyond the canonical boundaries of protein domains in general. | Conan K.Wang Lifeng Pan Jia Chen Mingjie Zhang | 2010 | Protein & Cell2010，1，8： | 1 |
| 11 | Preventive ma- intenance scheduling for repairable system with deteriora- tion显示文摘 | LIAO Wenzhu PAN Ershun XI Lifeng | 2010 | Journal of Intelligent Manufacturing2010，21，6： | 1 |
| 12 | Design for diagnosability of multistation manufacturing systems based on sensor allocation optimization显示文摘 | SUN Jiwen XI Lifeng PAN Ershun | 2009 | Computers in Industry2009，60，7： | 1 |
| 13 | Design for diagnosability of muhistation manufacturing systems based on sensor allocation optimization 显示文摘 | SUN Jiwen XI Lifeng PAN Er'shun | 2009 | Computers in Industry2009，69，7： | 1 |
| 14 | A maximum powerpoint tracking method combined with constant voltage tracking& variable step-size perturbation显示文摘 | ZHANG Kaisheng CHENG Pan HE Lifeng | 2014 | Sensors & Transducers2014，1757，： | 1 |
| 15 | A hybrid approach to reducing computed tomography metal artifacts in intracavitary brachytherapy 显示文摘 | Dan Xia Roeske John C Yu Lifeng Pelizzari Charles A Mundt Arno J and Pan Xiaochuan | 2005 | Brachytherapy2005，4，1： | 1 |
| 16 | Study and application on fluorescent spectrum of the inclusion complex of β-cyclodextrin and medroxyprogesterone acetate显示文摘 | Ma Hongyan Yao Lifeng Luo Zhaofu Pan Zuting Xu Mianyi | 1997 | Wuhan University Journal of Natural Sciences1997，，3： | 1 |
| 17 | A robust method of X-ray source spectrum estimation from transmission measurements:Demonstrated on computer simulated,scatter-free transmission data显示文摘 | Emil Y Sidky Lifeng Yu Xiaochuan Pan Yu Zou Michael Vannier | 2005 | Journal of Applied Physics2005，97，12： | 1 |
| 18 | Rapid profiling and identification of triterpenoid saponins in crude extracts from Albizia julibrissin Durazz. by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry显示文摘 | Lifeng Han Guixiang Pan Yuefei Wang Xinbo Song Xiumei Gao Baiping Ma Liping Kang | 2011 | Journal of Pharmaceutical and Biomedical Analysis2011，，5： | 1 |
| 19 | Structure-based development of potent and selective type-II kinase inhibitors of RIPK1显示文摘Receptor-interacting serine/threonine-protein kinase 1(RIPK1)functions as a key regulator in inflammation and cell death and is involved in mediating a variety of inflammatory or degenerative diseases.A number of allosteric RIPK1 inhibitors(RIPK1i)have been developed,and some of them have already advanced into clinical evaluation.Recently,selective RIPK1i that interact with both the allosteric pocket and the ATP-binding site of RIPK1 have started to emerge.Here,we report the rational development of a new series of type-II RIPK1i based on the rediscovery of a reported but mechanistically atypical RIPK3i.We also describe the structure-guided lead optimization of a potent,selective,and orally bioavailable RIPK1i,62,which exhibits extraordinary efficacies in mouse models of acute or chronic inflammatory diseases.Collectively,62 provides a useful tool for evaluating RIPK1 in animal disease models and a promising lead for further drug development. | Ying Qin Dekang Li Chunting Qi Huaijiang Xiang Huyan Meng Jingli Liu Shaoqing Zhou Xinyu Gong Ying Li Guifang Xu Rui Zu Hang Xie Yechun Xu Gang Xu Zheng Zhang Shi Chen Lifeng Pan Ying Li Li Tan | 2024 | Acta Pharmaceutica Sinica B2024，14，1： | 0 |
| 20 | Adaptive SPC monitoring scheme for DOE-based APC显示文摘Automatic process control (APC) based on design of experiment (DOE) is a cost-efficient approach for variation reduction. The process changes both in mean and variance owing to online parameter adjustment make it hard to apply traditional SPC charts in such DOE-based APC applied process. An adaptive SPC scheme is developed, which can better track the process transitions and achieve the possible SPC run cost reduction when the process is stable. The control law of SPC parameters is designed by fully utilizing the estimation properties of the process model instead of traditionally using the data collected from the production line. An example is provided to illustrate the proposed adaptive SPC design approach. | Ye Liang Pan Ershun Xi Lifeng | 2008 | Journal of Systems Engineering and Electronics2008，19，2： | 0 |
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