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| 1 | A structural view of the antibiotic degradation enzyme NDM-1 from a superbug显示文摘Gram-negative Enterobacteriaceae with resistance to carbapenem conferred by New Delhi metallo-β-lactamase 1(NDM-1)are a type of newly discovered antibioticresistant bacteria.The rapid pandemic spread of NDM-1 bacteria worldwide(spreading to India,Pakistan,Europe,America,and Chinese Taiwan)in less than 2 months characterizes these microbes as a potentially major global health problem.The drug resistance of NDM-1 bacteria is largely due to plasmids containing the blaNDM-1 gene shuttling through bacterial populations.The NDM-1 enzyme encoded by the blaNDM-1 gene hydrolyzes β-lactam antibiotics,allowing the bacteria to escape the action of antibiotics.Although the biological functions and structural features of NDM-1 have been proposed according to results from functional and structural investigation of its homologues,the precise molecular characteristics and mechanism of action of NDM-1 have not been clarified.Here,we report the threedimensional structure of NDM-1 with two catalytic zinc ions in its active site.Biological and mass spectroscopy results revealed that D-captopril can effectively inhibit the enzymatic activity of NDM-1 by binding to its active site with high binding affinity.The unique features concerning the primary sequence and structural conformation of the active site distinguish NDM-1 from other reported metallo-β-lactamases(MBLs)and implicate its role in wide spectrum drug resistance.We also discuss the molecular mechanism of NDM-1 action and its essential role in the pandemic of drug-resistant NDM-1 bacteria.Our results will provide helpful information for future drug discovery targeting drug resistance caused by NDM-1 and related metallo-β-lactamases. | Yu Guo Jing Wang Guojun Niu Wenqing Shui Yuna Sun Honggang Zhou Yaozhou Zhang Cheng Yang Zhiyong Lou Zihe Rao | 2011 | Protein & Cell2011，2，5： | 16 |
| 2 | Molecular mechanism of SCARB2-mediatec attachment and uncoating of EV71显示文摘 | Minghao Dang Xiangxi Wang Quan Wang Yaxin Wang Jianping Lin Yuna Sun Xuemei Li Liguo Zhang Zhiyong Lou Junzhi Wang Zihe Rao | 2014 | Protein & Cell2014，5，9： | 13 |
| 3 | Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1显示文摘 | Liming Yan Yuanyuan Ma Dan Liu Xiaochao Wei Yuna Sun Xiaoyue Chen Huadong Zhao Jingwen Zhou Zhiyong Wang Wenqing Shui Zhiyong Lou | 2012 | Cell Research2012，22，8： | 10 |
| 4 | 酶解乳蛋白对初生仔猪小肠组织学结构及隐窝细胞增殖的影响(英文)显示文摘INTRODUCTIONTheintestineasthemajorinterfacebetweenanorganismanditsnutritionalenvironmentplaysacriticalroleinpostnataldevelopm... | Tian Wang 1,Ruo Jun Xu 2, Yuna Qiang Zhang 3, Chong Tian Zheng 1 and Jian Cheng Zhang 4 1 Department of Animal Nutrition and Feed, Nanjing Agricultural University,210095 2 Department of Zoology, The University of Hong Kong 3 Department | 1999 | 中国组织化学与细胞化学杂志1999，8，2： | 5 |
| 5 | Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharideinduced inflammatory responses in broilers显示文摘Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune system.However,the underlying mechanisms by which they communicate with the host have not been clearly understood.Bacterial extracellular vesicles(EVs)have been considered as important mediators of host-pathogen interactions,but their potential role in commensals-host crosstalk has not been widely studied.Here,we investigated the regulatory actions of EVs produced by L.reuteri BBC3,a gut-associated commensal bacterium of Black-Bone chicken,in the development of lipopolysaccharide(LPS)-induced intestinal inflammation in a chicken model using both in vivo and in vitro experiments.Results:L.reuteri BBC3 produced nano-scale membrane vesicles with the size range of 60-250 nm.Biochemical and proteomic analyses showed that L.reuteri BBC3-derived EVs(LrEVs)carried DNA,RNA and several bioactive proteins previously described as mediators of other probiotics’beneficial effects such as glucosyltransferase,serine protease and elongation factor Tu.In vivo broiler experiments showed that administration of LrEVs exerted similar effects as L.reuteri BBC3 in attenuating LPS-induced inflammation by improving growth performance,reducing mortality and decreasing intestinal injury.LrEVs suppressed the LPS-induced expression of pro-inflammatory genes(TNF-α,IL-1β,IL-6,IL-17 and IL-8),and improved the expression of anti-inflammatory genes(IL-10 and TGF-β)in the jejunum.LrEVs could be internalized by chicken macrophages.In vitro pretreatment with LrEVs reduced the gene expression of TNF-α,IL-1βand IL-6 by suppressing the NF-κB activity,and enhanced the gene expression of IL-10 and TGF-βin LPS-activated chicken macrophages.Additionally,LrEVs could inhibit Th1-and Th17-mediated inflammatory responses and enhance the immunoregulatory cells-mediated immunosuppression in splenic lymphocytes of LPS-challenged chickens through the activation of macrophages.Finally,we revealed that the reduced content of both vesicular proteins and nucleic acids attenuated the suppression of LrEVs on LPS-induced inflammatory responses in ex vivo experiments,suggesting that they are essential for the LrEVs-mediated immunoregulation.Conclusions:We revealed that LrEVs participated in maintaining intestinal immune homeostasis against LPSinduced inflammatory responses in a chicken model.Our findings provide mechanistic insight into how commensal and probiotic Lactobacillus species modulate the host’s immune system in pathogens-induced inflammation. | Rujiu Hu Hua Lin Mimi Wang Yuezhen Zhao Haojing Liu Yuna Min Xiaojun Yang Yupeng Gao Mingming Yang | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 5 |
| 6 | The nucleoprotein of severe fever with thrombocytopenia syndrome virus processes a stable hexameric ring to facilitate RNA encapsidation显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),a member of the Phlebovirus genus from the Bunyaviridae family endemic to China,is the causative agent of life-threatening severe fever with thrombocyto-penia syndrome(SFTS),which features high fever and hemorrhage.Similar to other negative-sense RNA viruses,SFTSV encodes a nucleocapsid protein(NP)that is essen-tial for viral replication.NP facilitates viral RNA encapsida-tion and is responsible for the formation of ribonucleopro-tein complex.However,recent studies have indicated that NP from Phlebovirus members behaves in inhomogene-ous oligomerization states.In the present study,we report the crystal structure of SFTSV NP at 2.8Åresolution and demonstrate the mechanism by which it processes a ring-shaped hexameric form to accomplish RNA encapsida-tion.Key residues essential for oligomerization are identi-fi ed through mutational analysis and identifi ed to have a signifi cant impact on RNA binding,which suggests that correct formation of highly ordered oligomers is a criti-cal step in RNA encapsidation.The fi ndings of this work provide new insights into the discovery of new antiviral reagents for Phlebovirus infection. | Honggang Zhou Yuna Sun Ying Wang Min Liu Chao Liu Wenming Wang Xiang Liu Le Li Fei Deng Hualin Wang Yu Guo Zhiyong Lou | 2013 | Protein & Cell2013，4，6： | 4 |
| 7 | Synthesis,structure and luminescence properties of metal-organic frameworks based on benzo-bis(imidazole)显示文摘Metal-organic frameworks(MOFs)constructed from conjugated organic ligands are candidates for hybrid photoactive materials with potential applications.Compared to that from the ligands only,the intensity and wavelength of the luminescence could be tuned after they were incorporated in extended framework.In this report,by using an organic ligand with azolate moiety,benzo-bis(imidazole)(H2BBI),we synthesized two new MOF structures.Framework 1([Co(H2BBI)(DMSO)2Cl2]n,DMSO=dimethyl sulfoxide),constructed from tetrahedral Co(II)and H2BBI,exhibits zigzag 1D structure.Meanwhile,framework 2([Cu2(H2BBI)3(DMSO)6(NO3)4]n),a layered structure with hcb topology,was assembled from tetragonal pyramidal Cu(II)and H2BBI.Furthermore,2 exhibits strong luminescence emission(ex=280 nm).A blue shift of 40 nm(from 359 nm to 319 nm)was observed in framework 2 compared to the free ligand,which could be explained by the ligand-to-metal charge transfer in the network. | YIN XinBo SONG YuNa WANG Yang ZHANG LiJuan LI QiaoWei | 2014 | Science China Chemistry2014，57，1： | 4 |
| 8 | Kinetics of recovering germanium from lignite ash with chlorinating roasting methods显示文摘由使氯化烤恢复 Ge 的一个过程被提出。因为它的低沸腾点, GeCl4 与褐煤灰被分开并且恢复。运动分析显示使氯化烤过程与缩小模型的 unreacted 核心适合,反应率方程对应于 1 吗?2a/3?( 1?一) 2/3 = kt。明显的激活精力 Ea 被计算是在 pH = 的媒介的 22.36 kJ D301R 树脂 3.47。静态地浸透的吸附能力是 410 mg/g。在 D301R 树脂上吸附的磅(IV ) 能是由 1.0-2.0 mol/L NaOH 的 eluted。率常数是 k298 = 5. | ZHU Guocai WANG Jingyan CHENG Zhuo ZHAO Yuna | 2008 | Rare Metals2008，27，3： | 3 |
| 9 | Photonic integrated spiking neuron chip based on a self-pulsating DFB laser with a saturable absorber显示文摘We proposed and experimentally demonstrated a simple and novel photonic spiking neuron based on a distributed feedback(DFB)laser chip with an intracavity saturable absorber(SA).The DFB laser with an intracavity SA(DFBSA)contains a gain region and an SA region.The gain region is designed and fabricated by the asymmetric equivalentπ-phase shift based on the reconstruction-equivalent-chirp technique.Under properly injected current in the gain region and reversely biased voltage in the SA region,periodic self-pulsation was experimentally observed due to the Q-switching effect.The self-pulsation frequency increases with the increase of the bias current and is within the range of several gigahertz.When the bias current is below the self-pulsation threshold,neuronlike spiking responses appear when external optical stimulus pulses are injected.Experimental results show that the spike threshold,temporal integration,and refractory period can all be observed in the fabricated DFB-SA chip.To numerically verify the experimental findings,a time-dependent coupled-wave equation model was developed,which described the physics processes inside the gain and SA regions.The numerical results agree well with the experimental measurements.We further experimentally demonstrated that the weighted sum output can readily be encoded into the self-pulsation frequency of the DFB-SA neuron.We also benchmarked the handwritten digit classification task with a simple single-layer fully connected neural network.By using the experimentally measured dependence of the self-pulsation frequency on the bias current in the gain region as an activation function,we can achieve a recognition accuracy of 92.2%,which bridges the gap between the continuous valued artificial neural networks and spike-based neuromorphic networks.To the best of our knowledge,this is the first experimental demonstration of a photonic integrated spiking neuron based on a DFB-SA,which shows great potential to realizing large-scale multiwavelength photonic spiking neural network chips. | YUECHUN SHI SHUIYING XIANG XINGXING GUO YAHUI ZHANG HONGJI WANG DIANZHUANG ZHENG YUNA ZHANG YANAN HAN YONG ZHAO XIAOJUN ZHU XIANGFEI CHEN XUN LI YUE HAO | 2023 | Photonics Research2023，11，8： | 2 |
| 10 | An investigation on stability of biomass reduced manganese dioxide ore显示文摘In this study,how to improve the stability of reduced manganese oxide ore was discussed by investigating reoxidation conditions and kinetics mechanism in the cooling process of manganese dioxide ore reduced by biomass.The effects of the temperature and time,chip size of biomass,raw materials thickness and different additives on stability of the products were determined.The valence variation of manganese in ore and the reoxidation kinetics of reduced products were studied.The results show that decrease of reduction temperature and time,and increase of raw materials thickness and little H2SO4 additive are favorable for the stability of the reduced products.The kinetics mechanism of the reoxidation is controlled by diffusion with dynamic apparent activation energy of E1=25.10 kJ·mol-1,and conformation of manganese in the process is changed from MnO to Mn3O4. | Honglei ZHANG Guocai ZHU Hong YAN Yuna ZHAO Fangpu CHEN Wenjuan WANG | 2012 | Acta Metallurgica Sinica(English Letters)2012，25，6： | 2 |
| 11 | The Investigation of Argon Plasma Surface Modification to Polyethylene:Quantitative ATR-FTIR Spectroscopic Analysis显示文摘 | Shi Laishun Wang Luyan Wang Yuna | 2006 | European Polymer Journal2006，42，： | 1 |
| 12 | Effect of Sr content on porosity formation in directionally solidified Al-12.3wt.%Si alloy显示文摘The influence of Sr addition on pore formation in directionally solidified Al-12.3wt.% alloy was investigated using X-ray detection, optical microscope, and SEM-EDX. Results indicate that addition of Sr significantly increases the number density and volume fraction of porosity. The considerable rise in volume fraction of porosity is attributed to the remarkable increase in the numbers of pores formed. It is found that Sr solute in liquid Al-Si alloy can diffuse into the oxide inclusions to form loose oxide aggregations which have more activity as the nucleation sites for porosity. Adding more Sr considerably increases the numbers of active nucleation sites. There is an obvious fluctuation of pore number density during steady state solidification, which is believed to be related to a fluctuation of local hydrogen supersaturation induced by the competition of pore nucleation and growth for hydrogen solute supplement. | Liao Hengcheng Wu Yuna Fan Ran Wang Qigui | 2014 | China Foundry2014，11，5： | 1 |
| 13 | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl(BSN-GelMA)hydrogel显示文摘Autologous mosaicplasty is a common approach used to treat osteochondral defects in clinical practice.Gap integration between host and transplanted plugs requires bone tissue reservation and hyaline cartilage regeneration without uneven surface,graft necrosis and sclerosis.However,poor gap integration is a serious concern,which eventually leads to deterioration of joint function.To deal with such complications,this study has developed a strategy to effectively enhance integration of the gap region following mosaicplasty by applying injectable bioactive supramolecular nanofiber-enabled gelatin methacryloyl(GelMA)hydrogel(BSN-GelMA).A rabbit osteochondral defect model demonstrated that BSN-GelMA achieved seamless osteochondral healing in the gap region between plugs of osteochondral defects following mosaicplasty,as early as six weeks.Moreover,the International Cartilage Repair Society score,histology score,glycosaminoglycan content,subchondral bone volume,and collagen II expression were observed to be the highest in the gap region of BSN-GelMA treated group.This improved outcome was due to bio-interactive materials,which acted as tissue fillers to bridge the gap,prevent cartilage degeneration,and promote graft survival and migration of bone marrow mesenchymal stem cells by releasing bioactive supramolecular nanofibers from the GelMA hydrogel.This study provides a powerful and applicable approach to improve gap integration after autologous mosaicplasty.It is also a promising off-the-shelf bioactive material for cell-free in situ tissue regeneration. | Hongwei Wu Yuna Shang Wei Sun Xinyi Ouyang Wenyan Zhou Jieji Lu Shuhui Yang Wei Wei Xudong Yao g Xiaozhao Wang Xianzhu Zhang Yishan Chen Qiulin He Zhimou Yang Hongwei Ouyang | 2023 | Bioactive Materials2023，，1： | 1 |
| 14 | Uterine inflammation status modulates eggshell mineralization via calcium transport and matrix protein synthesis in laying hens显示文摘This study explored the effects of uterine inflammation on eggshell mineralization,ultrastructure and mechanical properties in laying hens modified by a lipopolysaccharide(LPS)challenge or dietary essential oil(EO)addition.In trial 1,a total of 72 Hy-line Brown layers at 36 wk of age were randomly assigned to 3 treatment groups(n=8),where they were intravenously injected with phosphate buffered saline,LPS at 1 mg/kg body weight,or LPS 3 times at 24-h intervals.In trial 2,a total of 288 Hy-line Brown layers at 60 wk of age were randomly divided into 4 groups(n=8),where they were fed basal diets supplemented with EO at 0,50,100 and 200 mg/kg for 12 wk.A uterine inflammation model was constructed with LPS treatment,indicated by the elevated expression of IL-1β and TNF-α(P<0.05)and lymphocyte infiltration.Uterine inflammation caused remarkable decreases in eggshell thickness and mechanical properties with structure deteriorations(P<0.05).Uterine inflammation stimulated the expression of matrix proteins ovotransferrin(TF)and ovalbumin(OVAL),while depressing the mRNA levels of calbindin-1(CALB1)and osteopontin in uterine mucosa(P<0.05).In contrast,EO addition alleviated uterine inflammation,evidenced by depressed levels of IL-1b and IL-6(P<0.05).There was a significant elevation in shell thickness and breaking strength following EO intervention(P<0.05),and these effects were maximized at addition of 100 mg/kg.Further,EO improved shell ultrastructure including more early fusion,less type B mammillae,and increased effective thickness(P<0.05).The alleviated inflammation decreased the expression of OVAL and TF,whereas ion transport genes like CALB1 and solute carrier family 26 member 9 were upregulated(P<0.05).Our findings suggest that inflam-matory status can impact uterine functions in calcium transport and the synthesis of matrix proteins especially such as OVAL and TF,which in turn modulates calcium precipitation and ultrastructure for-mation,thereby determining eggshell mechanical properties.These findings provide a novel insight into the uterine inflammation-mediated modifications of eggshell quality. | Jia Feng Mingyuan Lu Lingling Ma Haijun Zhang Shugeng Wu Kai Qiu Yuna Min Guanghai Qi Jing Wang | 2023 | Animal Nutrition2023，，2： | 1 |
| 15 | Electrochemical corrosion performance of Cr and Al alloy steels using a J55 carbon steel as base alloy显示文摘 | Rong Wang Sheji Luo Ming Liu Yuna Xue | 2014 | Corrosion Science2014，，： | 1 |
| 16 | Overexpression of CaWRKY27 , a subgroup IIe WRKY transcription factor of Capsicum annuum , positively regulates tobacco resistance to Ralstonia solanacearum infection显示文摘 | Fengfeng Dang Yuna Wang Jianju She Yufen Lei Zhiqin Liu Thomas Eulgem Yan Lai Jing Lin Lu Yu Dan Lei Deyi Guan Xia Li Qian Yuan Shuilin He | 2014 | Physiol Plantarum2014，，3： | 1 |
| 17 | The Investiga- tion of Argon Plasma Surface Modification to Polyethy- lene: Quantitative ATR-FTIR Spectroscopic Analysis 显示文摘 | Shi Laishun Wang Luyan Wang Yuna | 2006 | European Polymer Journal2006，42，7： | 1 |
| 18 | Photonic integrated neuro-synaptic core for convolutional spiking neural network显示文摘Neuromorphic photonic computing has emerged as a competitive computing paradigm to overcome the bottlenecks of the von-Neumann architecture.Linear weighting and nonlinear spike activation are two fundamental functions of a photonic spiking neural network(PSNN).However,they are separately implemented with different photonic materials and devices,hindering the large-scale integration of PSNN.Here,we propose,fabricate and experimentally demonstrate a photonic neuro-synaptic chip enabling the simultaneous implementation of linear weighting and nonlinear spike activation based on a distributed feedback(DFB)laser with a saturable absorber(DFB-SA).A prototypical system is experimentally constructed to demonstrate the parallel weighted function and nonlinear spike activation.Furthermore,a fourchannel DFB-SA laser array is fabricated for realizing matrix convolution of a spiking convolutional neural network,achieving a recognition accuracy of 87%for the MNIST dataset.The fabricated neuro-synaptic chip offers a fundamental building block to construct the large-scale integrated PSNN chip. | Shuiying Xiang Yuechun Shi Yahui Zhang Xingxing Guo Ling Zheng Yanan Han Yuna Zhang Ziwei Song Dianzhuang Zheng Tao Zhang Hailing Wang Xiaojun Zhu Xiangfei Chen Min Qiu Yichen Shen Wanhua Zheng Yue Hao | 2023 | Opto-Electronic Advances2023，6，11： | 1 |
| 19 | CD38 deficiency activates ERK1/2-NF-κB signaling pathway in sepsis-associated renal injury显示文摘CD38 is known to play roles in various inflammatory pathways.However,whether it has a protective or detrimental effect during bacterial septicemia remains disputed.Herein,this study aimed to determine the potential effect of CD38 on renal injury in septicemia.Escherichia coli(E.coli)was used to induce sepsis-associated renal injury in mice.WT and CD38-/-mice were stimulated with E.coli.After three hours,the serum was collected to detect renal function.Function mRNA expressions inflammatory cytokines in kidneys were quantified by real-time PCR.Hematoxylin and eosin staining were used to observe the histomorphology of kidney.The expression of TLR4,NF-κB,MAPK and cytokines were detected by Western Blot.Our results demonstrated that 3×10^(8) cfu/mL E.coli is the appropriate dose to induce sepsis mice model.Compared to WT sepsis mice,CD38-/-mice showed aggravated kidney injuries with impaired renal function,increased inflammation and apoptosis after E.coli stimulation.Interestingly,CD38 deficiency also led to elevated expression of TLR4 and increased phosphorylation of NF-κB p65/p105 and ERK1/2.To sum up,our results suggested that CD38 deficiency could aggravate E.coli-induced renal injury through activating ERK1/2-NF-κB signaling pathway. | ZHANG Huiqing DU Yuna XIE Zhengyu WANG Zeyu LI Hua 李蓉 | 2022 | 实验与检验医学2022，40，1： | 0 |
| 20 | Multi-omics analysis reveals the molecular regulatory network underlying the prevention of Lactiplantibacillus plantarum against LPS-induced salpingitis in laying hens显示文摘Background Salpingitis is one of the common diseases in laying hen production, which greatly decreases the economic outcome of laying hen farming. Lactiplantibacillus plantarum was effective in preventing local or systemic inflammation, however rare studies were reported on its prevention against salpingitis. This study aimed to investigate the preventive molecular regulatory network of microencapsulated Lactiplantibacillus plantarum(MLP) against salpingitis through multi-omics analysis, including microbiome, transcriptome and metabolome analyses.Results The results revealed that supplementation of MLP in diet significantly alleviated the inflammation and atrophy of uterus caused by lipopolysaccharide(LPS) in hens(P < 0.05). The concentrations of plasma IL-2 and IL-10 in hens of MLP-LPS group were higher than those in hens of LPS-stimulation group(CN-LPS group)(P < 0.05). The expression levels of TLR2, MYD88, NF-κB, COX2, and TNF-α were significantly decreased in the hens fed diet supplemented with MLP and suffered with LPS stimulation(MLP-LPS group) compared with those in the hens of CN-LPS group(P < 0.05). Differentially expressed genes(DEGs) induced by MLP were involved in inflammation, reproduction, and calcium ion transport. At the genus level, the MLP supplementation significantly increased the abundance of Phascolarctobacterium, whereas decreased the abundance of Candidatus_Saccharimonas in LPS challenged hens(P < 0.05). The metabolites altered by dietary supplementation with MLP were mainly involved in galactose, uronic acid, histidine, pyruvate and primary bile acid metabolism. Dietary supplementation with MLP inversely regulates LPSinduced differential metabolites such as Lyso PA(24:0/0:0)(P < 0.05).Conclusions In summary, dietary supplementation with microencapsulated Lactiplantibacillus plantarum prevented salpingitis by modulating the abundances of Candidatus_Saccharimonas, Phascolarctobacterium, Ruminococcus_torques_group and Eubacterium_hallii_group while downregulating the levels of plasma metabolites, p-tolyl sulfate, o-cresol and N-acetylhistamine and upregulating S-lactoylglutathione, simultaneously increasing the expressions of CPNE4, CNTN3 and ACAN genes in the uterus, and ultimately inhibiting oviducal inflammation. | Dan Song Aike Li Bingxu Chen Jia Feng Tao Duan Junlin Cheng Lixian Chen Weiwei Wang Yuna Min | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
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