|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Supramolecular Hydrogels of Indole-Capped Short Peptides as Vaccine Adjuvants显示文摘 | Zhongyan Wang Yanbin Cai Linan Yi Jie Gao Zhimou Yang | 2017 | Chinese Journal of Chemistry2017，35，7： | 2 |
| 2 | Tandem Molecular Self-Assembly Selectively Inhibits Lung Cancer Cells by Inducing Endoplasmic Reticulum Stress显示文摘The selective formation of nanomaterials in cancer cells and tumors holds great promise for cancer diagnostics and therapy.Until now,most strategies rely on a single trigger to control the formation of nanomaterials in situ.The combination of two or more triggers may provide for more sophisticated means of manipulation.In this study,we rationally designed a molecule(Comp.1)capable of responding to two enzymes,alkaline phosphatase(ALP),and reductase.Since the A549 lung cancer cell line showed elevated levels of extracellular ALP and intracellular reductase,we demonstrated that Comp.1 responded in a stepwise fashion to those two enzymes and displayed a tandem molecular self-assembly behavior.The selective formation of nanofibers in the mitochondria of the lung cancer cells led to the disruption of the mitochondrial membrane,resulting in an increased level of reactive oxygen species(ROS)and the release of cytochrome C(Cyt C).ROS can react with proteins,resulting in endoplasmic reticulum(ER)stress and the unfolded protein response(UPR).This severe ER stress led to disruption of the ER,formation of vacuoles,and ultimately,apoptosis of the A549 cells.Therefore,Comp.1 could selectively inhibit lung cancer cells in vitro and A549 xenograft tumors in vivo.Our study provides a novel strategy for the selective formation of nanomaterials in lung cancer cells,which is powerful and promising for the diagnosis and treatment of lung cancer. | Debin Zheng Yumiao Chen Sifan Ai Renshu Zhang Zhengfeng Gao Chunhui Liang Li Cao Yaoxia Chen Zhangyong Hong Yang Shi Ling Wang Xingyi Li Zhimou Yang | 2019 | Research2019，，1： | 1 |
| 3 | Enzyme-instructed and mitochondria-targeting peptide self-assembly to efficiently induce immunogenic cell death显示文摘Immunogenic cell death(ICD) plays a major role in cancer immunotherapy by stimulating specific T cell responses and restoring the antitumor immune system.However,effective type Ⅱ ICD inducers without biotoxicity are still very limited.Herein,a tentative drug-or photo sensitizer-free strategy was developed by employing enzymatic self-assembly of the peptide F-pY-T to induce mitochondrial oxidative stress in cancer cells.Upon dephosphorylation catalyzed by alkaline phosphatase overexpressed on cancer cells,the peptide F-pY-T self-assembled to form nanoparticles,which were subsequently internalized.These affected the morphology of mitochondria and induced serious reactive oxygen species production,causing the ICD characterized by the release of danger-associated molecular patterns(DAMPs).DAMPs enhanced specific immune responses by promoting the maturation of DCs and the intratumoral infiltration of tumor-specific T cells to eradicate tumor cells.The dramatic immunotherapeutic capacity could be enhanced further by combination therapy of F-pY-T and anti-PD-L1 agents without visible biotoxicity in the main organs.Thus,our results revealed an alternative strategy to induce efficient ICD by physically promoting mitochondrial oxidative stress. | Debin Zheng Jingfei Liu Limin Xie Yuhan Wang Yinghao Ding Rong Peng Min Cui Ling Wang Yongjie Zhang Chunqiu Zhang Zhimou Yang | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 1 |
| 4 | Enzymatic hydrogelation of small molecules 显示文摘 | Yang Zhimou Liang Gaolin Xu Bing | 2008 | Accounts of Chemical Research2008，41，： | 1 |
| 5 | Facet-Selective 2D Self-Assembly of TiO2 Nanoleaves via Supramolecular Interactions显示文摘 | Cheng Yang Zhimou Yang Hongwei Gu | 2008 | Chemistry of Materials2008，20，： | 1 |
| 6 | 显示文摘 | Zhang Yah Gu Hongwei Yang Zhimou | 2003 | Am Chem Soc2003，125，13： | 1 |
| 7 | Organelle-inspired supramolecular nanomedicine to precisely abolish liver tumor growth and metastasis显示文摘Organelles are responsible for the efficient storage and transport of substances in living systems.A myriad of extracellular vesicles(EVs)acts as a bridge to exchange signaling molecules in cell-cell communication,and the highly dynamic tubulins and actins contribute to efficient intracellular substance transport.The inexhaustible cues of natural cargo delivery by organelles inspire researchers to explore the construction of biomimetic architectures for“smart”delivery carriers.Herein,we report a 10-hydroxycamptothecin(HCPT)-peptide conjugate HpYss that simulates the artificial EV-to-filament transformation process for precise liver cancer therapy.Under the sequential stimulus of extracellular alkaline phosphatase(ALP)and intracellular glutathione(GSH),HpYss proceeds via tandem self-assembly with a morphological transformation from nanoparticles to nanofibers.The experimental phase diagram elucidates the influence of ALP and GSH contents on the self-assembled nanostructures.In addition,the dynamic transformation of organelle-mimetic architectures that are formed by HpYss in HepG2 cells enables the efficient delivery of the anticancer drug HCPT to the nucleus,and the size-shape change from extracellular nanoparticles(50-100 nm)to intracellular nanofibers(4-9 nm)is verified to be of key importance for nuclear delivery.Nuclear targeting of HpYss amplifies apoptosis,thus significantly enhancing the inhibitory effect of HCPT(>10-fold)to HepG2 cells.Benefitting from the spatiotemporally controlled nanostructures,HpYss exhibited deep penetration,enhanced accumulation,and long-term retention in multicellular spheroid and xenograft models,potently abolishing liver tumor growth and preventing lung metastasis.We envision that our organelle-mimicking delivery strategy provides a novel paradigm for designing nanomedicine to cancer therapy. | Jie Zhan Yuhan Wang Shaodan Ma Qin Qin Ling Wang Yanbin Cai Zhimou Yang | 2022 | Bioactive Materials2022，7，3： | 1 |
| 8 | 显示文摘 | Yang Zhimou Wang Ling Xu Bing | 2010 | Matter Chem2010，20，11： | 1 |
| 9 | Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets显示文摘This paper was to determine the effects of dietary Litsea cubeba essential oil(LEO)supplementation on growth performance,immune function,antioxidant level,intestinal morphology and microbial composition in weaned piglets.One hundred and ninety-two piglets(Duroc×[Large White×Landrace])with 6.85±0.22 kg mean body weight weaned at 21 d of age were randomly assigned to 4 treatment groups with 8 replicates and were fed with a basal diet(CON)or CON diet containing 100(LLEO),200(MLEO)and 400(HLEO)mg/kg LEO.The results revealed that HLEO supplementation(P<0.05)increased the average daily gain on d 28 compared with CON.MLEO and HLEO supplementation decreased(P<0.05)feed conversion ratio.LEO-containing diets had a lower(P<0.05)diarrhea rate.Supple-mentation with HLEO increased(P<0.05)total antioxidant capacity(T-AOC)both in the serum and liver.Meanwhile,the supplementation of MLEO and HLEO resulted in higher(P<0.05)glutathione peroxidase(GPx)activities both in serum and liver.Supplementation of HLEO increased(P<0.05)serum immu-noglobulin A,immunoglobulin G and interleukin-10,whereas supplementation with MLEO and HLEO decreased(P<0.05)tumor necrosis factor-α.Villus height in the duodenum or jejunum was increased(P<0.05)in the HLEO group,and the villus height to crypt depth ratio in the jejunum was also improved(P<0.05)in the MLEO group.The addition of LEO increased(P<0.05)the richness and diversity of the microbial community in the cecum,which mainly increased the relative abundance of Oscillospir-aceae_UCG-005,Faecalibacterium,Blautia and Coprococcus.Piglets supplemented with HLEO increased(P<0.05)the concentration of short chain fatty acids(SCFA),including acetic acid in the cecum and propionic acid in the colon.In conclusion,these findings indicated that LEO supplementation improved growth performance and intestinal health in weaned piglets. | Zhe Yang Fang Wang Yexin Yin Peng Huang Qian Jiang Zhimou Liu Yulong Yin Jiashun Chen | 2023 | Animal Nutrition2023，，2： | 1 |
| 10 | Preorganization boosts the artificial esterase activity of a self-assembling peptide显示文摘The creation of artificial enzymes to mimic natural enzymes remains a great challenge owing to the complexity of the structural arrangement of the essential amino acids in catalytic centers.In this study,we used the phosphatase-based enzyme-instructed self-assembly(EISA)to supervise artificial esterases'final structures and catalytic activities.We reported that peptide precursors containing different phosphorylation sites could preorganize into alternated nanostructures and undergo dephosphorylation in the presence of alkaline phosphatase(ALP)with variation in kinetic and thermodynamic profiles.Although identical self-assembly compositions were formed after dephosphorylation,precursors with more enhanced preorganized states tended to better promote ALP dephosphorylation,facilitate further self-assembly,and strengthen the catalytic activities of the final assemblies.We envisioned that our strategy would be useful for further construction and manipulation of various artificial enzymes with superior catalytic activities. | Yaoxia Chen Wenwen Zhang Yinghao Ding Chunhui Liang Yang Shi Zhi-Wen Hu Ling Wang Zhimou Yang | 2021 | Science China Chemistry2021，64，9： | 1 |
| 11 | Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl(BSN-GelMA)hydrogel显示文摘Autologous mosaicplasty is a common approach used to treat osteochondral defects in clinical practice.Gap integration between host and transplanted plugs requires bone tissue reservation and hyaline cartilage regeneration without uneven surface,graft necrosis and sclerosis.However,poor gap integration is a serious concern,which eventually leads to deterioration of joint function.To deal with such complications,this study has developed a strategy to effectively enhance integration of the gap region following mosaicplasty by applying injectable bioactive supramolecular nanofiber-enabled gelatin methacryloyl(GelMA)hydrogel(BSN-GelMA).A rabbit osteochondral defect model demonstrated that BSN-GelMA achieved seamless osteochondral healing in the gap region between plugs of osteochondral defects following mosaicplasty,as early as six weeks.Moreover,the International Cartilage Repair Society score,histology score,glycosaminoglycan content,subchondral bone volume,and collagen II expression were observed to be the highest in the gap region of BSN-GelMA treated group.This improved outcome was due to bio-interactive materials,which acted as tissue fillers to bridge the gap,prevent cartilage degeneration,and promote graft survival and migration of bone marrow mesenchymal stem cells by releasing bioactive supramolecular nanofibers from the GelMA hydrogel.This study provides a powerful and applicable approach to improve gap integration after autologous mosaicplasty.It is also a promising off-the-shelf bioactive material for cell-free in situ tissue regeneration. | Hongwei Wu Yuna Shang Wei Sun Xinyi Ouyang Wenyan Zhou Jieji Lu Shuhui Yang Wei Wei Xudong Yao g Xiaozhao Wang Xianzhu Zhang Yishan Chen Qiulin He Zhimou Yang Hongwei Ouyang | 2023 | Bioactive Materials2023，，1： | 1 |
| 12 | γ‐Ray‐Responsive Supramolecular Hydrogel Based on a Diselenide‐Containing Polymer and a Peptide显示文摘 | Wei Cao Xiaoli Zhang Xiaoming Miao Zhimou Yang Huaping Xu | 2013 | Angew Chem Int Ed2013，，24： | 1 |
| 13 | Organic silica/Nafion ? composite membrane for direct methanol fuel cells显示文摘 | Suzhen Ren Gongquan Sun Chennan Li Zhenxing Liang Zhimou Wu Wei Jin Xin Qin Xuefeng Yang | 2006 | Journal of Membrane Science2006，，1： | 1 |
| 14 | Heterodimers of nanoparticles:Formation at a liquid-liquid interface and particlespecific surface modification by functional molecules显示文摘 | Gu Hongwei Yang Zhimou | 2005 | J Am Chem Soc2005，127，1： | 1 |
| 15 | An amelogenin-based peptide hydrogel promoted the odontogenic differentiation of human dental pulp cells显示文摘Amelogenin can induce odontogenic differentiation of human dental pulp cells(HDPCs),which has great potential and advantages in dentine-pulp complex regeneration.However,the unstability of amelogenin limits its further application.This study constructed amelogenin self-assembling peptide hydrogels(L-gel or D-gel)by heating-cooling technique,investigated the effects of these hydrogels on the odontogenic differentiation of HDPCs and explored the underneath mechanism.The critical aggregation concentration,conformation,morphology,mechanical property and biological stability of the hydrogels were characterized,respectively.The effects of the hydrogels on the odontogenic differentiation of HDPCs were evaluated via alkaline phosphatase activity measurement,quantitative reverse transcription polymerase chain reaction,western blot,Alizarin red staining and scanning electron microscope.The mechanism was explored via signaling pathway experiments.Results showed that both the L-gel and D-gel stimulated the odontogenic differentiation of HDPCs on both Day 7 and Day 14,while the D-gel showed the highest enhancement effects.Meanwhile,the D-gel promoted calcium accumulation and mineralized matrix deposition on Day 21.The D-gel activated MAPK-ERK1/2 pathways in HDPCs and induced the odontogenic differentiation via ERK1/2 and transforming growth factor/smad pathways.Overall,our study demonstrated that the amelogenin peptide hydrogel stimulated the odontogenic differentiation and enhanced mineralization,which held big potential in the dentine-pulp complex regeneration. | Xinxin Li Zhaoxia Yu Shihui Jiang Xiaohua Dai Guanhua Wang Yue Wang Zhimou Yang Jie Gao Huiru Zou | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 16 | Imaging cellular distribution of fluorescent supramolecular nanofibers显示文摘In this study, we used the 4-nitro-2,1,3-benzoxadiazole(NBD) as an aromatic capping group for a peptide to construct the supramolecular nanofibers. Taking the advantage of the fluorescence property of NBD, we could directly observe the cellular distribution of the self-assembled nanofibers. We found that the distributions of the nanofibers of NBD-FFETIGGY are different in four mammalian cells and two plant cells. The nanofibers are mainly located at the surface of two mammalian cells and one plant cell, while in the intracellular space of other cells. Different distributions of nanofibers lead to different protein binding patterns of the nanofibers in two different cell lines. We believe that a useful and versatile platform has been offered to the image cellular distribution of nanofibers, which can provide useful information to the biological functions of the self-assembled nanostructures. | Jingyu Wang Jing Zheng Yanbin Cai Jinxin Zheng Jie Gao Qingqiu Gong Zhimou Yang | 2016 | Science China Chemistry2016，59，6： | 0 |
| 17 | The protective effects of a D-tetra-peptide hydrogel adjuvant vaccine against H7N9 influenza virus in mice显示文摘Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans,with mortality reaching approximately 40%–50%.To prevent possible pandemics,the development of highly effective vaccines against influenza virus H7N9 is highly desired.In the present study,by taking advantage of the D-tetra-peptide adjuvant(G^(D)F^(D)F^(D)Y),we reported a simple method to prepare H7N9 vaccines.Naproxen(Npx),with good anti inflammatory and broad anti-viral effects,was employed as an N-terminal capping group to construct a hydrogel precursor,Npx-G^(D)F^(D)F^(D)Y.The hydrogel adjuvant was prepared using a routine heating cooling protocol and the final vaccine was ready after mixing with the split A/Zhejiang/DTID-ZJU01/2013(H7N9)antigen by vortexing.Compared with the traditional Al(OH)_(3) adjuvant vaccine and the split vaccine,our hydrogel adjuvant vaccine showed the best preventive effects against H7N9 infection.A mechanistic study illustrated that higher antibody responses and variations in cytokine expression might account for its increased protective effects.Our strategy demonstrated the advantages of a peptide hydrogel adjuvant in the application of vaccines against H7N9 and demonstrated its potential application in vaccines against emerging threats from other viruses. | Keda Chen Xiaoxin Wu Qingjing Wang Ying Wang Haiyan Zhang Shuai Zhao Chaonan Li Zhiwen Hu Zhimou Yang Lanjuan Li | 2023 | Chinese Chemical Letters2023，34，1： | 0 |
| 18 | Supramolecular hydrogels of self-assembled zwitterionic-peptides显示文摘Zwitterionic polymer materials have been extensively studied,but zwitterionic peptides supramolecular hydrogel materials are rarely studied.In this study,the preparation of two zwitterionic hydrogels using self-assembled peptides were reported.The hydrogels could be fabricated easily by changing the temperature or enzyme catalysis in a short time.And the differences in structure and function of the zwitterion peptide hydrogels caused by the two preparation methods were also be compared.We found that the hydrogel prepared by enzyme induced self-assembly has better solubility and lower cytotoxicity than that prepared by the heating-cooling process.The result showed the enzyme induced self-assembly way to form zwitterionic peptides supramolecular hydrogel materials could have further biomedical applications. | Yiming Zhang Yinghao Ding Xinxin Li Debin Zheng Jie Gao Zhimou Yang | 2021 | Chinese Chemical Letters2021，32，11： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员