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| 1 | Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize显示文摘In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome. | WANG FengGe ZHAO JiuRan DAI JingRui YI HongMei KUANG Meng SUN YanMei YU XinYan GUO JingLun WANG Lu | 2007 | Chinese Science Bulletin2007，52，2： | 35 |
| 2 | Biosorption mechanisms involved in immobilization of soil Pb by Bacillus subtilis DBM in a multi-metal-contaminated soil显示文摘Mechanisms of soil Pb immobilization by Bacillus subtilis DBM, a bacterial strain isolated from a heavy-metal-contaminated soil, were investigated. Adsorption and desorption experiments with living bacterial cells as well as dead cells revealed that both extracellular adsorption and intracellular accumulation were involved in the Pb2+removal from the liquid phase. Of the sequestered Pb(II), 8.5% was held by physical entrapment within the cell wall, 43.3% was held by ion-exchange, 9.7% was complexed with cell surface functional groups or precipitated on the cell surface, and 38.5% was intracellularly accumulated.Complexation of Pb2+with carboxyl, hydroxyl, carbonyl, amido, and phosphate groups was demonstrated by Fourier transform infrared spectroscopic analysis. Precipitates of Pb5(PO4)3OH, Pb5(PO4)3Cl and Pb10(PO4)6(OH)2that formed on the cell surface during the biosorption process were identified by X-ray diffraction analysis. Transmission electron microscopy–energy dispersive spectroscopic analysis confirmed the presence of the Pb(II)precipitates and that Pb(II) could be sequestered both extracellularly and intracellularly.Incubation with B. subtilis DBM significantly decreased the amount of the weak-acid-soluble Pb fraction in a heavy-metal-contaminated soil, resulting in a reduction in Pb bioavailability, but increased the amount of its organic-matter-bound fraction by 71%. The ability of B.subtilis DBM to reduce the bioavailability of soil Pb makes it potentially useful for bacteria-assisted phytostabilization of multi-heavy-metal-contaminated soil. | Jun Bai Xiuhong Yang Ruiying Du Yanmei Chen Shizhong Wang Rongliang Qiu | 2014 | Journal of Environmental Sciences2014，26，10： | 22 |
| 3 | Human umbilical cord-derived mesenchymal stem cell therapy in patients with COVID-19:a phase 1 clinical trial显示文摘No effective drug treatments are available for coronavirus disease 2019(COVID-19).Host-directed therapies targeting the underlying aberrant immune responses leading to pulmonary tissue damage,death,or long-term functional disability in survivors require clinical evaluation.We performed a parallel assigned controlled,non-randomized,phase 1 clinical trial to evaluate the safety of human umbilical cord-derived mesenchymal stem cells(UC-MSCs)infusions in the treatment of patients with moderate and severe COVID-19 pulmonary disease.The study enrolled 18 hospitalized patients with COVID-19(n=9 for each group).The treatment group received three cycles of intravenous infusion of UC-MSCs(3×107 cells per infusion)on days 0,3,and 6.Both groups received standard COVID-treatment regimens.Adverse events,duration of clinical symptoms,laboratory parameters,length of hospitalization,serial chest computed tomography(CT)images,the PaO2/FiO2 ratio,dynamics of cytokines,and IgG and IgM anti-SARS-CoV-2 antibodies were analyzed.No serious UC-MSCs infusion-associated adverse events were observed.Two patients receiving UC-MSCs developed transient facial flushing and fever,and one patient developed transient hypoxia at 12 h post UC-MSCs transfusion.Mechanical ventilation was required in one patient in the treatment group compared with four in the control group.All patients recovered and were discharged.Our data show that intravenous UC-MSCs infusion in patients with moderate and severe COVID-19 is safe and well tolerated.Phase 2/3 randomized,controlled,double-blinded trials with long-term follow-up are needed to evaluate the therapeutic use of UC-MSCs to reduce deaths and improve long-term treatment outcomes in patients with serious COVID-19. | Fanping Meng Ruonan Xu Siyu Wang Zhe Xu Chao Zhang Yuanyuan Li Tao Yang Lei Shi Junliang Fu Tianjun Jiang Lei Huang Peng Zhao Xin Yuan Xing Fan Ji-Yuan Zhang Jinwen Song Dawei Zhang Yanmei Jiao Limin Liu Chunbao Zhou Markus Maeurer Alimuddin Zumla Ming Shi Fu-Sheng Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 16 |
| 4 | Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome显示文摘Hutchinson-Gilford 早衰症候群(HGPS ) 和沃纳症候群(WS ) 是二最好描绘的人的 progeroid 症候群。HGPS 被一个点变化在 lamin A (LMNA ) 基因引起,导致截断的蛋白质 productprogerin 的生产。WS 被变化在 WRN 基因引起,编码 loss-of-function RecQ DNA helicase。这里,由基因编辑,我们创造了 isogenic 人的胚胎的干细胞(转换字符) 与异质接合(G608G/+) 或同型结合(G608G/G608G ) LMNA 变化和 biallelic WRN 大美人为为 HGPS 和 WS 致病建模,分别地。当转换字符和 endothelial 房间(EC ) 没介绍早熟的老朽的任何特征时, HGPS 间充质、 WS 间充质的干细胞(MSC ) 与不同动力学显示出联系老化的显型。当 HGPS-MSCs 展出了迟了发作的尖锐早衰 characterisitcs 时, WS-MSCs 有早发作的温和早衰显型。一起拿,我们的学习比较并且形成对照不同病理 underpinning 二早衰混乱,并且提供可靠干细胞为病理学、生理的老化识别新治疗学的策略的基于的模型。 | Zeming Wu Weiqi Zhang Moshi Song Wei Wang Gang Wei Wei Li Jinghui Lei Yu Huang Yanmei Sang Piu Chan Chang Chen Jing Qu Keiichiro Suzuki Juan Carlos Izpisua Belmonte Guang-Hui Liu | 2018 | Protein & Cell2018，9，4： | 15 |
| 5 | Boceprevir,GC-376,and calpain inhibitors Ⅱ,Ⅻ inhibit SARS-CoV-2 viral replication by targeting the viral main protease显示文摘A new coronavirus SARS-CoV-2,also called novel coronavirus 2019(2019-nCoV),started to circulate among humans around December 2019,and it is now widespread as a global pandemic.The disease caused by SARS-CoV-2 virus is called COVID-19,which is highly contagious and has an overall mortality rate of 6.35%as of May 26,2020.There is no vaccine or antiviral available for SARS-CoV-2.In this study,we report our discovery of inhibitors targeting the SARS-CoV-2 main protease(Mpro).Using the FRET-based enzymatic assay,several inhibitors including boceprevir,GC-376,and calpain inhibitorsⅡ,andⅫwere identified to have potent activity with single-digit to submicromolar ICs0 values in the enzymatic assay.The mechanism of action of the hits was further characterized using enzyme kinetic studies,thermal shift binding assays,and native mass spectrometry.Significantly,four compounds(boceprevir,GC-376,calpain inhibitorsⅡandⅫ)inhibit SARS-CoV-2 viral replication in cell culture with EC50 values ranging from 0.49 to 3.37μM.Notably,boceprevir,calpain inhibitorsⅡandⅫrepresent novel chemotypes that are distinct from known substrate-based peptidomimetic Mpro inhibitors.A complex crystal structure of SARS-CoV-2 Mpro with GC-376,determined at 2.15(A)resolution with three protomers per asymmetric unit,revealed two unique binding configurations,shedding light on the molecular interactions and protein conformational flexibility underlying substrate and inhibitor binding by Mpro.Overall,the compounds identified herein provide promising starting points for the further development of SARS-CoV-2 therapeutics. | Chunlong Ma Michael Dominic Sacco Brett Hurst Julia Alma Townsend Yanmei Hu Tommy Szeto Xiujun Zhang Bart Tarbet Michael Thomas Marty Yu Chen Jun Wang | 2020 | Cell Research2020，30，8： | 13 |
| 6 | Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer显示文摘Metastasis,the development of secondary malignant growths at a distance from a primary tumor,is the cause of death for 90%of cancer patients,but little is known about how metastatic cancer cells adapt to and colonize new tissue environments.Here,using clinical samples,patient-derived xenograft(PDX)samples,PDX cells,and primary/metastatic cell lines,we discovered that liver metastatic colorectal cancer(CRC)cells lose their colon-specific gene transcription program yet gain a liver-specific gene transcription program.We showed that this transcription reprogramming is driven by a reshaped epigenetic landscape of both typical enhancers and super-enhancers.Further,we identified that the liver-specific transcription factors FOXA2 and HNF1A can bind to the gained enhancers and activate the liver-specific gene transcription,thereby driving CRC liver metastasis.Importantly,similar transcription reprogramming can be observed in multiple cancer types.Our data suggest that reprogrammed tissue-specific transcription promotes metastasis and should be targeted therapeutically. | Shuaishuai Teng Yang Eric Li Ming Yang Rui Qi Yiming Huang Qianyu Wang Yanmei Zhang Shanwen Chen Shasha Li Kequan Lin Yang Cao Qunsheng Ji Qingyang Gu Yujing Cheng Zai Chang Wei Guo Pengyuan Wang Ivan Garcia-Bassets Zhi John Lu Dong Wang | 2020 | Cell Research2020，30，1： | 11 |
| 7 | A comparative study of MEA and DEA for post-combustion CO2 capture with different process configurations显示文摘 | Boyang Xue Yanmei Yu Jian Chen Xiaobo Luo Meihong Wang | 2017 | International Journal of Coal Science & Technology2017，4，1： | 11 |
| 8 | Solar flare forecasting using learning vector quantity and unsupervised clustering techniques显示文摘In this paper, a combined method of unsupervised clustering and learning vector quantity (LVQ) is presented to forecast the occurrence of solar flare. Three magnetic parameters including the maximum horizontal gradient, the length of the neutral line, and the number of singular points are extracted from SOHO/MDI longitudinal magnetograms as measures. Based on these pa- rameters, the sliding-window method is used to form the sequential data by adding three days evolutionary information. Con- sidering the imbalanced problem in dataset, the K-means clustering, as an unsupervised clustering algorithm, is used to convert imbalanced data to balanced ones. Finally, the learning vector quantity is employed to predict the flares level within 48 hours. Experimental results indicate that the performance of the proposed flare forecasting model with sequential data is improved. | LI Rong WANG HuaNing CUI YanMei HUANG Xin | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，8： | 10 |
| 9 | Influence of Nitrogen Flow Ratio on the Microstructure, Composition, and Mechanical Properties of DC Magnetron Sputtered Zr-B-O-N Films显示文摘Nanocrystalline ZrB 2 film and nanocomposite Zr-B-O-N films were prepared by non-reactive as well as reactive magnetron sputtering techniques, respectively. By means of X-ray diffraction analysis, electron probe microanalysis, X-ray photoelectron spectroscopy, and scanning electron microscopy, the influence of nitrogen flow ratio on the film microstructure and characteristics were investigated systematically, including the deposition rate, chemical compositions, phase constituents, grain size, chemical bonding, as well as cross-sectional morphologies. Meanwhile, the hardness and adhesion of above films were also evaluated by micro-indentation method and a scratch tester. With increasing the nitrogen flow ratio, the deposition rate of above films decreased approximately linearly, whereas the contents of N and O in the films increased gradually and tended to saturation. Moreover, the film microstructure was also altered gradually from a fine columnar microstructure to a featureless glass-structure. As the nitrogen flow ratio was 11.7%, the Zr-B-O-N film possessed an typical nanocomposite structure and presented good mechanical properties. During the process of reactive sputtering of metal borides, the introduction of nitrogen can show a pronounced suppression of columnar grain growth and strong nanocomposite structure forming ability. | Tie-Gang Wang Yanmei Liu Tengfei Zhang Doo-In Kim KwanKHo Kim | 2012 | Journal of Materials Science & Technology2012，28，11： | 10 |
| 10 | Effect of Cyclocarya paliurus polysaccharides on streptozotocin-induced diabetic nephropathy in rats显示文摘OBJEVTIVE:To investigate the efficacy of Cyclocarya paliurus(C.paliurus)polysaccharides on streptozotocin-induced diabetic nephropathy in rats.METHODS:Rats were divided into 6 groups,including group of normal control,group of diabetic control,group of metformin treatment,low-dose group of C.paliurus polysaccharides treatment,middle-dose group of C.paliurus polysaccharides treatment and high-dose group of C.paliurus polysaccharides treatment.Histological analysis of kidney was analyzed using hematoxilin and eosin.Levels of blood glucose,creatinine,urea,uric acid were determined by spectrophotometry.Anti-oxidative enzymes were measured by real-time polymerase chain reaction(PCR)and enzyme-linked immunosorbent assay(ELISA).Advanced glycation end products(AGEs)and transforming growth factor-β1(TGF-β1)level was measured by ELISA.RESULTS:Abnormal changes were observed in the group of diabetic control characterized by atrophy of the renal glomeruli with hypercellularity,congestion of glomerular tufts,dilation of the renal spaces,and degeneration of renal tubule.Compared with that of normal group,blood glucose,creatinine,urea,uric acid level was significantly increased in the group of diabetic control.Superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase level was significantly decreased,but AGEs and TGF-β1 level was significantly increased.By contrast,administration of C.paliurus polysaccharides and metformin could reverse the above-mentioned results of the group of diabetic control,especially in the high-dose group of C.paliurus polysaccharides.CONCLUSION:Our findings suggest that C.paliurus polysaccharides may play a protecting role for nephropathy of diabetic rats by lowering glucose,creatinine,urea,uric acid level,enhancing the antioxidative ability,and reducing AGEs and TGF-β1 expression. | Xia Xichao Mao Dongxue Dai Hongmei Wu Xi Zhang Zuyuan Wang Huaying Zhou Wenwen Dong Yanmei Wang Mengqi Li Yuan Shao Xiangyang Ouyang Jingfeng | 2020 | Journal of Traditional Chinese Medicine2020，40，6： | 9 |
| 11 | 3D C_f/SiBCN composites prepared by an improved polymer infiltration and pyrolysis显示文摘Using liquid poly(methylvinyl)borosilazanes(PMVBSZ)as precursor,carbon fiber reinforced SiBCN matrix composites(C_f/SiBCN)were fabricated by a modified polymer infiltration and pyrolysis(PIP)process.With dicumyl peroxide added as cross-linking agent,the PMVBSZ could be solidified at a low temperature of 120℃,leading to a high ceramic yield of~70%.The cross-linking mechanism and ceramization processes of the precursor were investigated in detail.Moreover,a modified infiltration technology was developed,which improved the efficiency and protected the precursor against moist air during PIP.Consequently,the obtained C_f/SiBCN composites had an oxygen content of around 1.22 wt%.Benefiting from the high ceramic yield and high efficiency of the modified PIP,C_f/SiBCN composites with an open porosity of~10%and uniform microstructure were obtained after only 7 cycles of PIP.The flexural strength and fracture toughness of the derived C_f/SiBCN composites were 371 MPa and 12.9 MPa·m^(1/2),respectively.This work provides a potential route for the fabrication of high performance C_f/SiBCN composites. | Qi DING Dewei NI Zhen WANG Hao ZHONG Hongda WANG Haijun ZHOU Yanmei KAN Shaoming DONG | 2018 | Journal of Advanced Ceramics2018，7，3： | 9 |
| 12 | Depositional architecture of the late Ordovician drowned carbonate platform margin and its responses to sea-level fluctuation in the northern slope of the Tazhong region, Tarim Basin显示文摘The Tazhong Uplift of the late Ordovician is a drowned rimmed carbonate platform. The carbonate rock of the late Ordovician Lianglitage Formation in the northern slope of the Tazhong region is one of the significant petroliferous intervals. Based on petrofacies, depositional cycles, natural gammaray spectrometry and carbon/oxygen isotope data from the Lianglitage Formation, one 2nd-order, three 3rd-order and several 4th-order sequences have been recognized, and the late Ordovician relative sealevel fluctuation curve has been established. The sequences O 3 l-1 and O 3 l-2 on the platform are composed of highstand and transgressive systems tracts, but lack the lowstand systems tract. The sequence O 3 l-3 is a drowning sequence. The sequence O 3 l-1 overlapped the eroded slope and pinched out to the northwest and landward. The highstand systems tract in the sequence O 3 l-2 consists of low-angle sigmoid and high-angle shingled progradation configuration. Major sedimentary facies of the Lianglitage Formation include reef and shoal in the platform margin and lagoon, which can be subdivided into coral-sponge-stromatoporoid reef complex, sand shoal, lime mud mound, and intershoal sea. Reefs, sand shoals and their complex are potential reservoir facies. The reefs and sand shoals in the sequence O 3 l-1 developed in the upper of its highstand systems tract. In the sequence O 3 l-2, the highstand systems tract with an internal prograding configuration is a response to the lateral shifting of the complex of reef and sand shoal. The transgressive systems tract, in particular the sand shoals, developed widely on the slope of the platform margin and interior. The reefs in the sequence O 3 l-3 migrated towards high positions and formed retrograding reefs in the western platform and low relief in the platform interior. Basinward lateral migration of the reefs and pure carbonate rock both characterize highstand systems tract and show that the rise of the relative sea-level was very slow. Shingled prograding stacking pattern of the 4th-order sequences and reefs grow horizontally, which represents the late stage of highstand systems tract and implies relative sealevel stillstand. Reefs migrating towards high land and impure carbonate rock both indicate transgressive systems tract and suggest that the relative sea-level rose fast. Erosional truncation and epidiagenetic karstification represent a falling relative sea-level. The relative sea-level fluctuation and antecedent palaeotopography control the development and distribution of reef complexes and unconformity karst zones. Currently, the composite zone of epidiagenetic karstic intervals and high-energy complexes of reefs and sand shoals with prograding configuration is an important oil and gas reservoir in the northern slope of the Tazhong carbonate platform. | Yang Xiaofa Lin Changsong Yang Haijun Han Jianfa Liu Jingyan Zhang Yanmei Peng Li Jing Bing Tong Jianyu Wang Haiping Li Huanpu | 2010 | Petroleum Science2010，7，3： | 8 |
| 13 | Formation of disinfection byproducts in typical Chinese drinking water显示文摘Eight typical drinking water supplies in China were selected in this study.Both source and tap water were used to investigate the occurrence of chlorinated disinfection byproducts(DBPs),and seasonal variation in the concentrations of trihalomethanes(THMs) of seven water sources was compared.The results showed that the pollution level for source water in China,as shown by DBP formation potential,was low.The most encountered DBPs were chloroform,dichloroacetic acid,trichloroacetic acid,and chlorodibromoacetic acid.The concentration of every THMs and haloacetic acid(HAA) compound was under the limit of standards for drinking water quality.The highest total THMs concentrations were detected in spring. | Wenbo Liu Yanmei Zhao Christopher WK Chow Dongsheng Wang | 2011 | Journal of Environmental Sciences2011，23，6： | 7 |
| 14 | Disinfection byproduct precursor removal by enhanced coagulation and their distribution in chemical fractions显示文摘Raw water from the Songhua River was treated by four types of coagulants, ferric chloride(FeCl3), aluminum sulfate(Al2(SO4)3),polyaluminum chloride(PACl) and composite polyaluminum(HPAC), in order to remove dissolved organic matter(DOM). Considering the disinfection byproduct(DBP) precursor treatability, DOM was divided into five chemical fractions based on resin adsorption.Trihalomethane formation potential(THMFP) and haloacetic acid formation potential(HAAFP) were measured for each fraction. The results showed that hydrophobic acids(HoA), hydrophilic matter(HiM) and hydrophobic neutral(HoN) were the dominant fractions.Although both HoN and HoA were the main THM precursors, the contribution for THMFP changed after coagulation. Additionally,HoA and HiM were the main HAA precursors, while the contribution of HoN to HAAFP significantly increased after coagulation.HoM was more easily removed than HiM, no matter which coagulant was used, especially under enhanced coagulation conditions.DOC removal was highest for enhanced coagulation using FeCl3 while DBPFP was lowest using PACl. This could indicate that not all DOC fractions contained the precursors of DBPs. Reduction of THMFP and HAAFP by PACl under enhanced coagulation could reach51% and 59% respectively. | Yanmei Zhao Feng Xiao Dongsheng Wang Mingquan Yan Zhe Bi | 2013 | Journal of Environmental Sciences2013，25，11： | 7 |
| 15 | Genome-wide identification and characterization of phospholipase C gene family in cotton (Gossypium spp.)显示文摘Phospholipase C (PLC) are important regulatory enzymes involved in several lipid and Ca2+-dependent signaling pathways.Previous studies have elucidated the versatile roles of PLC genes in growth, development and stress responses of many plants, however, the systematic analyses of PLC genes in the important fiber-producing plant, cotton, are still deficient. In this study,through genome-wide survey, we identified twelve phosphatidylinositol-specific PLC (PI-PLC) and nine non-specific PLC (NPC) genes in the allotetraploid upland cotton Gossypium hirsutum and nine PI-PLC and six NPC genes in two diploid cotton G. arboretum and G.raimondii, respectively. The PI-PLC and NPC genes of G. hirsutum showed close phylogenetic relationship with their homologous genes in the diploid cottons and Arabidopsis. Segmental and tandem duplication contributed greatly to the formation of the gene family. Expression profiling indicated that few of the PLC genes are constitutely expressed, whereas most of the PLC genes are preferentially expressed in specific tissues and abiotic stress conditions. Promoter analyses further implied that the expression of these PLC genes might be regulated by MYB transcription factors and different phytohormones.These results not only suggest an important role of phospholipase C members in cotton plant development and abiotic stress response but also provide good candidate targets for future molecular breeding of superior cotton cultivars. | Bing Zhang Yanmei Wang Jin-Yuan Liu | 2018 | Science China(Life Sciences)2018，61，1： | 7 |
| 16 | High-throughput sequencing-based genome-wide identification of micro RNAs expressed in developing cotton seeds显示文摘Micro RNAs(mi RNAs) have been shown to play critical regulatory roles in gene expression in cotton. Although a large number of mi RNAs have been identified in cotton fibers, the functions of mi RNAs in seed development remain unexplored. In this study, a small RNA library was constructed from cotton seeds sampled at 15 days post-anthesis(DPA) and was subjected to high-throughput sequencing. A total of 95 known mi RNAs were detected to be expressed in cotton seeds. The expression pattern of these identified mi RNAs was profiled and 48 known mi RNAs were differentially expressed between cotton seeds and fibers at 15 DPA. In addition, 23 novel mi RNA candidates were identified in 15-DPA seeds. Putative targets for 21 novel and 87 known mi RNAs were successfully predicted and 900 expressed sequence tag(EST) sequences were proposed to be candidate target genes, which are involved in various metabolic and biological processes, suggesting a complex regulatory network in developing cotton seeds. Furthermore, mi RNA-mediated cleavage of three important transcripts in vivo was validated by RLM-5′ RACE. This study is the first to show the regulatory network of mi RNAs that are involved in developing cotton seeds and provides a foundation for future studies on the specific functions of these mi RNAs in seed development. | WANG YanMei DING Yan YU DingWei XUE Wei LIU JinYuan | 2015 | Science China(Life Sciences)2015，58，8： | 7 |
| 17 | Identification of differentially expressed proteins in poplar leaves in-duced by Marssonina brunnea f. sp. Multigermtubi显示文摘Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar (M. brunnea) interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone 'NL895' (P. euramericana CL'NL895'), which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. mul- tigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE. About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Ma- trix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and un- classified proteins. | Kun Yuan Bo Zhang Yanmei Zhang Qiang Cheng Mingxiu Wang Minren Huang | 2008 | Journal of Genetics and Genomics2008，35，1： | 6 |
| 18 | Characterization of nanoscale iron and its degradation of 2,4-dichlorophenol显示文摘Nanoscale iron was detected by TEM, X-ray diffraction and X-ray photoelectron spectroscopy. It was found that the size of the nanoscale iron particles is in the range of 30?40 nm according to TEM image, and it contains abundant Fe3O4 as passivating layers on the surface of the core-shell structure. To improve its performance, dilute HCl was used for the removal of the passivating layers, and the degradation of 2,4-dichlorophenol (2,4-DCP) was measured for the nanoscale iron samples treated and untreated. Experimental results demonstrated that the removal of 2,4-DCP by untreated nanoscale iron is mainly due to the adsorption of 2, 4-DCP by nanoscale iron, and there are no degradation products detected by HPLC in the process. However, excellent dechlorination of 2,4-DCP was gained by HCl-treated nanoscale iron, and 2-chlorophenol, 4-chlorophenol and phenol were detected during the process. It was concluded that dechlorination is the key reaction pathway for the degradation of 2,4-DCP by activated nanoscale iron, and phenol is found to be the main product. | HOU ChunFeng GE XiaoPeng ZHOU YanMei LI Yi WANG DongSheng TANG HongXiaoI | 2010 | Chinese Science Bulletin2010，55，4： | 6 |
| 19 | pH modeling for maximum dissolved organic matter removal by enhanced coagulation显示文摘Correlations between raw water characteristics and pH after enhanced coagulation to maximize dissolved organic matter(DOM) removal using four typical coagulants(FeCl3,Al2(SO4)3,polyaluminum chloride(PACl) and high performance polyaluminum chloride(HPAC)) without pH control were investigated.These correlations were analyzed on the basis of the raw water quality and the chemical and physical fractionations of DOM of thirteen Chinese source waters over three seasons.It was found that the final pH after enhanced coagulation for each of the four coagulants was in?uenced by the content of removable DOM(i.e.hydrophobic and higher apparent molecular weight(AMW) DOM),the alkalinity and the initial pH of raw water.A set of feed-forward semi-empirical models relating the final pH after enhanced coagulation for each of the four coagulants with the raw water characteristics were developed and optimized based on correlation analysis.The established models were preliminarily validated for prediction purposes,and it was found that the deviation between the predicted data and actual data was low.This result demonstrated the potential for the application of these models in practical operation of drinking water treatment plants. | Jiankun Xie Dongsheng Wang John van Leeuwen Yanmei Zhao Linan Xing Christopher W. K. Chow | 2012 | Journal of Environmental Sciences2012，24，2： | 5 |
| 20 | Design and fabrication of a SiN-Si dua卜layer optical phased array chip显示文摘A SiN-Si dual-layer optical phased array(OPA)chip is designed and fabricated.It combines the low loss of SiN with the excellent modulation performance of Si,which improves the performance of Si single-layer OPA.A novel optical antenna and an improved phase modulation method are also proposed,and a two dimensional scanning range of 96°×14°is achieved,which makes the OPA chip more practical. | PENGFEI WANG GUANGZHEN LUO YANG XU YAJIE LI YANMEI SU JIANBIN MA RUITING WANG ZHENGXIA YANG XULIANG ZHOU YEJIN ZHANG JIAOQING PAN | 2020 | Photonics Research2020，8，6： | 5 |
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