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| 1 | Reactive dividing wall column for hydrolysis of methyl acetate:Design and control显示文摘Reactive distillation and dividing wall column distillation are two kinds of effective separation technologies,and their integrated configuration,reactive dividing wall column(RDWC),presents attractive advantages.In this study,the rigorous simulation of RDWC for methyl acetate hydrolysis is performed,and sensitivity analysis is conducted to obtain the minimum reboiler duty.Then a comparison is made between the conventional process and RDWC process,and it shows that 20.1% energy savings can be achieved by RDWC process.In addition,the dynamic characteristic of RDWC is studied and an effective control strategy is proposed.The simple PI control scheme with three temperature loops can obtain reasonable control performance and maintain products at desired purities.It is proved that this RDWC process is an energy efficiency alternative with good controllability. | Lumin Li Lanyi Sun Delian Yang Wang Zhong Yi Zhu Yuanyu Tian | 2016 | Chinese Journal of Chemical Engineering2016，24，10： | 11 |
| 2 | Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle显示文摘mRNA is a novel class of therapeutic modality that holds great promise in vaccination,protein replacement therapy,cancer immunotherapy,immune cell engineering etc.However,optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application.Here we present an ionizable lipid nanoparticle(iLNP)based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs.mRNAs encoding luciferase or erythropoietin(EPO)were prepared by in vitro transcription and formulated with proposed iLNP,to form iLP171/mRNA formulations.It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes.The maximum amount of protein expression was found at 6 h post-administration.The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA.Moreover,no toxicity or immunogenicity was observed for these mRNA formulations.Therefore,our study provides a useful and promising platform for mRNA therapeutic development. | Tongren Yang Chunhui Li Xiaoxia Wang Deyao Zhao Mengjie Zhang Huiqing Cao Zicai Liang Haihua Xiao Xing-Jie Liang Yuhua Weng Yuanyu Huang | 2020 | Bioactive Materials2020，5，4： | 7 |
| 3 | Fig fruit ripening is regulated by the interaction between ethylene and abscisic acid显示文摘Fleshy fruit ripening is typically regulated by ethylene in climacteric fruits and abscisic acid(ABA)in non-climacteric fruits.Common fig(Ficus carica)shows a dual-ripening mechanism,which is not fully understood.Here,we detected separate peaks of ethylene and ABA in fig fruits at the onset-and onripening stages,in conjunction with a sharp rise in glucose and fructose contents.In a newly-designed split-fruit system,exogenous ethylene failed to rescue fluridone-inhibited fruit ripening,whereas exogenous ABA rescued 2-amino-ethoxy-vinyl glycine(AVG)-inhibited fruit ripening.Transcriptome analysis revealed changes in the expression of genes key to both ABA and ethylene biosynthesis and perception during fig fruit ripening.At the degreening stage,downregulation of FcACO2 or FcPYL8 retarded ripening,but downregulation of FcETR1/2 did not;unexpectedly,downregulation of FcAAO3 promoted ripening,but it inhibited ripening only before the de-greening stage.Furthermore,we detected an increase in ethylene emissions in the FcAAO3-RNAi ripening fruit and a decrease in ABA levels in the FcACO2-RNAi unripening fruit.Importantly,FcPYL8 can bind to ABA,suggesting that it functions as an ABA receptor.Our findings support the hypothesis that ethylene regulates the fig fruit ripening in an ABA-dependent manner.We propose a model for the role of the ABA-ethylene interaction in climacteric/non-climacteric processes. | Han Qiao Han Zhang Zhun Wang Yuanyue Shen | 2021 | Journal of Integrative Plant Biology2021，63，3： | 5 |
| 4 | Advances of nanoparticles as drug delivery systems for disease diagnosis and treatment显示文摘Decades have passed since the first nanoparticles-base medicine was approved for human cancer treatment, and the research and development of nanoparticles for drug delivery are always undergoing.Nowadays, the significant advances complicate nanoparticles’ branches, including liposomes, solid lipid nanoparticles, inorganic nanoparticles, micelles, nanovaccines and nano-antibodies, etc. These nanoparticles show numerous capabilities in treatment and diagnosis of stubborn diseases like cancer and neurodegenerative diseases, emerging as novel drug carriers or therapeutic agents in future. In this review, the complicated branches of nanoparticles are classified and summarized, with their property and functions concluded. Besides, there are also some delivery strategies that make nanoparticles smarter and more efficient in drug delivery, and frontiers in these strategies are also summarized in this review. Except these excellent works in newly-produced drug delivery nanoparticles, some points of view and future expectations are made in the end. | Rui Liu Cong Luo Zhiqing Pang Jinming Zhang Shaobo Ruan Meiying Wu Lei Wang Tao Sun Nan Li Liang Han Jinjin Shi Yuanyu Huang Weisheng Guo Shaojun Peng Wenhu Zhou Huile Gao | 2023 | Chinese Chemical Letters2023，34，2： | 2 |
| 5 | Ionizable lipid-assisted efficient hepatic delivery of gene editing elements for oncotherapy显示文摘CRISPR/Cas9-based gene editing has emerged as a powerful biotechnological tool,that relies on Cas9 protein and single guided RNA(sgRNA)to edit target DNA.However,the lack of safe and efficient delivery carrier is one of the crucial factors restricting its clinical transformation.Here,we report an ionizable lipid nanoparticle(iLP181,pKa=6.43)based on iLY1809 lipid enabling robust gene editing in vitro and in vivo.The iLP181 effectively encapsulate psgPLK1,the best-performing plasmid expressing for both Cas9 protein and sgRNA targeting Polo-like kinase 1(PLK1).The iLP181/psgPLK1 nanoformulation showed uniformity in size,regular nanostructure and nearly neutral zeta potential at pH 7.4.The nanoformulation effectively triggered editing of PLK1 gene with more than 30%efficiency in HepG2-Luc cells.iLP181/psgPLK1 significantly accumulated in the tumor for more than 5 days after a single intravenous injection.In addition,it also achieved excellent tumor growth suppression compared to other nucleic acid modalities such as siRNA,without inducing adverse effects to the main organs including the liver and kidneys.This study not only provides a clinically-applicable lipid nanocarrier for delivering CRISPR/Cas system(even other bioactive molecules),but also constitutes a potential cancer treatment regimen base on DNA editing of oncogenes. | Chunhui Li Tongren Yang Yuhua Weng Mengjie Zhang Deyao Zhao Shuai Guo Bo Hu Wanxuan Shao Xiaoxia Wang Abid Hussain Xing-Jie Liang Yuanyu Huang | 2022 | Bioactive Materials2022，7，3： | 2 |
| 6 | 显示文摘 | Wang yuanyu Wu Jiagang Xiao Dingquan | 2007 | J Alloys Compd2007，08，： | 1 |
| 7 | Electrical properties and temperature stability of a new kind of lead-free piezoelectric ceramics 显示文摘 | WANG Yuanyu WU Jiagang XIAO Dingquan | 2008 | J Phys D: Appl Phys2008，41，24： | 1 |
| 8 | 显示文摘 | Wu Jiagang Xiao Dingquan Wang Yuanyu | 2007 | Appl Phys Lett2007，91，25： | 1 |
| 9 | Effects of Ag content on the phase structure niobium lead-free ceramics显示文摘 | Wu Jiagang Xiao Dingquan Wang Yuanyu | 2007 | Journal of Applied Physics2007，102，05： | 1 |
| 10 | 显示文摘 | Wang Yuanyu Wu Jiagang Xiao Dingquan | | J Alloy Compd0，，： | 1 |
| 11 | The Mg-chelatase H subunit is an abscisic acid receptor显示文摘 | SHEN Yuanyue WANG Xiaofang WU Fuqing | 2006 | Nature2006，443，： | 1 |
| 12 | Different atomic structures observed from ternary Ni-Nb-Ta metallic glasses obtained by ion beam mixing显示文摘Four sets of ternary Ni-Nb-Ta multilayered samples with overall compositions of Ni69Nb8Ta23,Ni55Nb13Ta32,Ni42Nb16Ta42 and Ni29Nb18Ta53,respectively were prepared and subjected to 185 keV xenon ion beam mixing.The experimental results showed that in the four Ni-Nb-Ta multilayered samples,metallic glasses could all be obtained at appropriate doses,supporting the prediction directly from a proven realistic Ni-Nb-Ta interatomic potential through molecular dynamics simulations,and that two different atomic structures were observed,as in the corresponding selected area diffraction patterns,the locations of the diffused bands reflected from the metallic glass phases were observed at different angles for the Ni69Nb8Ta23 and Ni29Nb18Ta53 metallic glasses.Interestingly,Voronoi tellessation analysis indicated that the observed difference in atomic structures could be attributed to the distinct coordinate number spectra,i.e.,the spectrum of the Ni69Nb8Ta23 metallic glass has its coordinate number(CN) equal to 13 as dominating atomic configuration(with a weight of about 27%),whereas for the Ni29Nb18Ta53 metallic glass,CN=14 is the dominating atomic configuration(also about 27%).Moreover,the distinct atomic configurations of the obtained Ni-Nb-Ta metallic glasses could be correlated to the structures of the constituent metals of the ternary Ni-Nb-Ta system,as the first neighbor of fcc is 12 and the sum of the first and second neighbors of bcc is 14,implying the structural heredity did play a role in metallic glass formation. | WANG YuanYu LIU JianBo DAI Ye LIU BaiXin | 2013 | Science China(Technological Sciences)2013，56，8： | 1 |
| 13 | The Role of Surface Oxygen in the Growth of Large Single-Crystal Graphene on Copper显示文摘 | Yufeng Hao M. S. Bharathi Lei Wang Yuanyue Liu Hua Chen Shu Nie Xiaohan Wang Harry Chou Cheng Tan Babak Fallahazad H. Ramanarayan Carl W. Magnuson Emanuel Tutuc Boris I. Yakobson Kevin F. McCarty Yong-Wei Zhang Philip Kim James Hone Luigi Colombo Rodney S | 2013 | 2013 (6159)2013，，6159： | 1 |
| 14 | The microgravity enhanced polymer-mediated siRNA gene silence by improving cellular uptake显示文摘Microgravity(MG)effect is a weightlessness phenomenon caused by the distance from the ground or low gravity of other planets outside the earth's atmosphere.The various effects of MG have been corroborated in human and animal studies and modeled in cell-based analogs.However,the impact of MG on siRNA performance remains to be elucidated,which is crucial for aerospace medicine.In this study,we prepared nucleic acid nanomicelles(EAASc/siRNA)by using tri-block copolymer of PEG45-PAMA40-P(C7A36-DBA37)(EAASc)and siRNA and explored its working mechanism under simulated microgravity(SMG)condition generated by a random positioning machine(RPM).The binding ability of EAASc to siRNA and silence activity were firstly confirmed in normal gravity(NG)environment.Evaluation of PLK1 mRNA expression revealed that gene inhibition efficiencies were increased by 28.7%(HepG2)and 28.9%(A549)under SMG condition,compared with those under NG condition.In addition,mechanism exploration indicated that morphology and migration capability of cancer cells were significantly changed,the internalization of EAASc/siRNA by cells was magnified when the cells were incubated with RPM.No significant difference was observed regarding the expression profiles of genes involved in RNA interference(RNAi)pathway,including Ago2,Dicer,TRBP,and so on.Taken together,siRNA activity was elevated under SMG condition owning to increased cellular internalization.This study,for the first time to our knowledge,provides valuable theory for development and application of siRNA therapeutic in space in the future. | Tongren Yang Chanchan Yu Changrong Wang Chunhui Li Mengjie Zhang Xiaofan Luo Yuhua Weng Anjie Dong Xiaoqiong Li Yulin Deng Yuanyu Huang | 2020 | Biophysics Reports2020，6，6： | 1 |
| 15 | Sustainable development index of shale gas exploitation in China, the UK, and the US显示文摘While shale gas could complement the world's natural gas supply,its environmental tradeoffs and sustainability potential should be cautiously assessed before using it to satisfy future energy needs.Shale gas development in China is still in its infancy but has been progressing by the Central Government at a fast pace nowadays.Advanced experience from North America would greatly benefit sustainable design and decision-making for energy development in China.However,the lack of consistency concerning internal and external parameters among previous investigations does not allow an integrated impact comparison among shale gas-rich countries.Herein,we applied a meta-analysis to harmonize environmental tradeoff data through a comprehensive literature review.Greenhouse gas emission,water consumption,and energy demand were selected as environmental tradeoff indicators during shale gas production.Data harmonization suggested that environmental tradeoffs ranged from 5.6 to 37.4 g CO_(2)-eq,11.0-119.7 mL water,and 0.027-0.127 MJ energy to produce 1 MJ shale gas worldwide.Furthermore,sustainable development indexes(SDIs)for shale gas exploitation in China were analyzed and compared to the United States and the United Kingdom by considering environment,economy,and social demand through an analytic hierarchy process.The United States and China elicit higher SDIs than the United Kingdom,indicating higher feasibility for shale gas exploitation.Although China has relatively low scores in the environmental aspect,large reservoirs and high future market demand make Chinese shale gas favorable in the social demand aspect.Region-specific SDI characteristics identified among representative countries could improve the sustainability potential of regional development and global energy supply. | Liang Li Fan Wu Yuanyu Cao Fei Cheng Dali Wang Huizhen Li Zhiqiang Yu Jing You | 2022 | Environmental Science and Ecotechnology2022，，4： | 0 |
| 16 | Discussion and Research on Orthopedic Biomechanics Testing Technology显示文摘With the continuous development of today's science and technology,orthopedic research has also achieved continuous updates in materials and machinery.In this case,the mechanics testing technology of orthopedics also needs to be further updated and developed,so that it can effectively meet the requirement for today's orthopedic mechanical testing.Based on this,this article analyzes several advanced orthopedic mechanics testing techniques.It is hoped that this analysis can provide a reference for the good application and development of orthopedic mechanics testing technology. | Jinkui Liang Wanzong Liu Xiuxiu Wang Yuanyu Li | 2020 | Journal of Clinical and Nursing Research2020，4，6： | 0 |
| 17 | Effect of Initial Concentrations on Solidification of Ammonium Chloride Water Solution显示文摘The effect of initial concentrations on solidification of ammonium chloride water solution is numerically investigated in detail.The solidifying process.with the cold wall temperature lower than the eutectic temperature,is assumed to be one-dimensional,and controlled by heat conduction only.The simulation reveals that:(1) The solid-mush interface grows in a linear manner,while the growth rate of the mush-liquid interface decreases in a parabolic manner,with increasing initial concentrations.(2) The temperature field in the whole region has parabolic characteristics,but is shows a linear feature in the solid zone and mushy zone.(3) The concentration always has linear characteristics in the much.(4)The solid fraction distribution is strongly affected by the initial concertration.The solidification process shows quite different features,especially at small and high initial concentrations. | Yuanyue Jin (1) Qijie Wang (1) Zhongqi Chen (1) | 1992 | Journal of Thermal Science1992，1，4： | 0 |
| 18 | Production of human neurotrophin-3 in baculovirus expression system显示文摘Human NT3 gene was inserted into pPAK9 and the resultant plasmid was used to cotransfect sf21 cells with Bsu36 Ⅰ digested Bac6 genomic DNA. Recombinant baculovirus was isolated by plaque assay. Slothybridization, immunocytochemistry and serumfree culture of DRG demonstrated that NT3 had been integrated into Bac6 DNA, expressed and secreted. The yield of purified hNT3 was 200 μg from 200 mL cultural supernatant after passing CM Sepharose Fast Flow column, the concentration for its maximum bioactivity was 4 ng/mL. | Yuanyue Mu Hong Liu Jie Zhang Shuhong Liu Yan Wu Jiazheng Wang Ming Fan | 1998 | Chinese Science Bulletin1998，43，18： | 0 |
| 19 | Interactions between H_(2)O_(2)and dissolved organic matter during granular activated carbon-based residual H_(2)O_(2)quenching from the upstream UV/H_(2)O_(2)process显示文摘Granular activated carbon(GAC)filtration can be employed to synchronously quench residual H_(2)O_(2)from the upstream UV/H_(2)O_(2)process and further degrade dissolved organicmatter(DOM).In this study,rapid small-scale column tests(RSSCTs)were performed to clarify the mechanisms underlying the interactions between H_(2)O_(2)and DOM during the GAC-based H_(2)O_(2)quenching process.It was observed that GAC can catalytically decompose H_(2)O_(2),with a long-lasting high efficiency(>80%for approximately 50,000 empty-bed volumes).DOM inhibited GAC-based H_(2)O_(2)quenching via a pore-blocking effect,especially at high concentrations(10 mg/L),with the adsorbed DOM molecules being oxidized by the continuously generated·OH;this further deteriorated the H_(2)O_(2)quenching efficiency.In batch experiments,H_(2)O_(2)could enhance DOM adsorption by GAC;however,in RSSCTs,it deteriorated DOM removal.This observation could be attributed to the different·OH exposure in these two systems.It was also observed that aging with H_(2)O_(2)and DOM altered the morphology,specific surface area,pore volume,and the surface functional groups of GAC,owing to the oxidation effect of H_(2)O_(2)and·OH on the GAC surface as well as the effect of DOM.Addi-tionally,the changes in the content of persistent free radicals in the GAC samples were insignificant following different aging processes.This work contributes to enhancing understanding regarding the UV/H_(2)O_(2)-GAC filtration scheme,and promoting the application in drinking water treatment. | Yaoyao Kang Junfeng Lian Yichun Zhu Zuwen Liu Wentao Li Huiyu Dong Yuanyue Wang Jinfeng Zeng Zhimin Qiang | 2023 | Journal of Environmental Sciences2023，，6： | 0 |
| 20 | A wave-shaped electrode flexible sensor capable of sensitively responding to wrinkle excitation for a multifunctional human-computer interaction system显示文摘Human-machine interactions(HMIs)have advanced rapidly in recent decades in the fields of healthcare,work,and life.However,people with disabilities and other mobility problems do not have corresponding high-tech aids for them to enjoy the convenience of HMIs.In this paper,we propose a sensor with a wave-shaped(corrugated)electrode embedded in a friction layer,which exhibits high sensitivity to skin fold excitation and enormous potential in HMIs.Attributing to the wave-shaped electrode design,it has no built-in cavities,and its small size allows it to flexibly cope with folds at different angles.By specifying the carbon nanotube hybrid silicone film as the electrode layer material and silicone film as the friction layer,good electrical output performance,tensile properties,and biocompatibility can be achieved.Then,the sensor is tested on various joints and skin folds of the human body,the output signals of which can be distinguished between normal physiological behavior and test behavior.Based on this sensor,we designed a medical alarm system,a robotic arm assistive system,and a cell phone application control system for the disabled to help them in the fields of healthcare,work,and life.In conclusion,our research presents a feasible technology to enhance HMIs and makes a valuable contribution to the development of high-tech aids for the disabled. | Yongyang Chen Zhiyi Wu Chengcheng Han Zhi Cao Yiran Hu Ping Zhao Yuanyu Wang | 2024 | Nano Research2024，17，5： | 0 |
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