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| 1 | Breast cancer stem-like cells can promote metastasis by activating platelets and down-regulating antitumor activity of natural killer cells显示文摘OBJECTIVE:To investigate whether cancer stem cells(CSCs) more efficiently activating platelets and evading immune surveillance than non-CSCs thus promoting metastasis.METHODS:We enriched and identified sphereforming cells(SFCs) and coincubated washed platelets with several platelet activators including collagen,4T1 and SFCs.Platelet-coating tumor cells,platelet activation and TGF-β1 release were analyzed.Then natural kell cells(NK) were incubated with supernatants of different activated platelet samples what we called sample release(SR).The degranulation assay and NKG2 D expression on NK cells were conducted by flow cytometry.Finally tissue factor(TF) expression of SFCs or 4T1 were evaluated by western blot.RESULTS:Breast cancer cell line 4T1 could form spheres in serum-free medium at low adherence.Sphere-forming cells expressed high levels of the CD24-/lowCD44 + stem cell phenotype.Both sphere-forming cells or 4T1 were coated with abundant platelets while sphere-forming cells induced significantly higher expression of platelet activating receptor CD62 p than 4T1 did(P < 0.01).And sphere-forming cells induced platelets to produce more TGF-β1 than 4T1 did(P < 0.01).Furthermore,sample releases induced by sphere-forming cells caused more vigorous inhibition of NK cells antitumor reactivity(P < 0.05) and reduced NKG2 D expression(P < 0.01).The final results showed that sphere-forming cells expressed higher levels of TF than 4T1(P < 0.05).CONCLUSION:Our findings indicate that CSCs could efficiently activate platelets,induce platelets to secrete more TGF-β1,decrease NKG2 D expression and inhibit antitumor activity of NK cell,compared with 4T1.And higher levels of TF expression of CSCs may account for this correlation of CSCs and platelets. | Wang Shuo Zhang Ying Cong Weihong Liu Jie Zhang Yuren Fan Huiting XuYonggang Lin Hongsheng | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 5 |
| 2 | Prevalence of High-Risk Human Papillomavirus in Cervical Intraepithelial Neoplasia in the Pre-Vaccine Era—China,2017–2018显示文摘What is already known about this topic?Human papillomavirus(HPV)type-specific strategies play a key role in the prevention of cervical intraepithelial neoplasia(CIN),but evidence on the HPV type-specific prevalence in CIN is limited.What is added by this report?This multicentric study estimates the prevalence of high-risk HPV types in CIN cases and the associations of HPV infection patterns with high-grade CIN in China in 2017–2018.What are the implications for public health practice?Population-based prevention strategies should give preference to HPV-16 and 18,and strategies for non-16/18 high-risk HPV are essential for the elimination of cervical cancer. | Heling Bao Weihong Chen Xiaosong Zhang Hui Bi Yun Zhao Liwen Fang Shu Cong Feng Tan Linhong Wang | 2022 | China CDC weekly2022，4，48： | 1 |
| 3 | Recommendations for the diagnosis and treatment of paroxysmal kinesigemc dyskinesia: an expert consensus in China显示文摘Paroxysmal dyskinesias are a group of neurological diseases characterized by intermittent episodes of involuntary movements with different causes.Paroxysmal kinesigenic dyskinesia(PKD)is the most common type of paroxysmal dyskinesia and can be divided into primary and secondary types based on the etiology.Clinically,PKD is characterized by recurrent and transient attacks of involuntary movements precipitated by a sudden voluntary action.The major cause of primary PKD is genetic abnormalities,and the inheritance pattern of PKD is mainly autosomal-dominant with incomplete penetrance.The proline-rich transmembrane protein 2(PRRT2)was the first identified causative gene of PKD,accounting for the majority of PKD cases worldwide.An increasing number of studies has revealed the clinical and genetic characteristics,as well as the underlying mechanisms of PKD.By seeking the views of domestic experts,we propose an expert consensus regarding the diagnosis and treatment of PKD to help establish standardized clinical evaluation and therapies for PKD.In this consensus,we review the clinical manifestations,etiology,clinical diagnostic criteria and therapeutic recommendations for PKD,and results of genetic analyses in PKD patients performed in domestic hospitals. | Li Cao Xiaojun Huang Ning Wang Zhiying Wu Cheng Zhang Weihong Gu Shuyan Cong Jianhua Ma Ling Wei Yanchun Deng Qi Fang Qi Niu Jin Wang Zhaoxia Wang You Yin Jinyong Tian Shufen Tian Hongyan Bi Hong Jiang Xiaorong Liu Yang Lu Meizhen Sun Jianjun Wu Erhe Xu Tao Chen Tao Chen Xu Chen Wei Li Shujian Li Qinghua Li Xiaonan Song Ying Tang Ping Yang Yun Yang Min Zhang Xiong Zhang Yuhu Zhang Ruxu Zhang Yi Ouyang Jintai Yu Quanzhong Hu Qing Ke Yuanrong Yao Zhe Zhao Xiuhe Zhao Guohua Zhao Furu Liang Nan Cheng Jianhong Han Rong Peng Shengdi Chen Beisha Tang | 2021 | Translational Neurodegeneration2021，10，1： | 1 |
| 4 | The investigation of miscibility in blends of ENR/AO-80 by DMA and FTIR显示文摘 | Li Cong Cao Deming Guo Weihong | 2008 | Journal of Macromolecular Science(part B):Physics2008，47，1： | 1 |
| 5 | Physical and degradation properties of binary or ternary blends composed of poly (lactic acid), thermoplastic starch and GMA grafted POE显示文摘 | Qingfeng Shi Cong Chen Lei Gao Lei Jiao Haiyan Xu Weihong Guo | 2010 | Polymer Degradation and Stability2010，，1： | 1 |
| 6 | Protective effects of oleic acid and polyphenols in extra virgin olive oil on cardiovascular diseases显示文摘The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which have been shown to have noteworthy nutritional value and positive impact on human health.It is worth noting that EVOO owes its superior nutritional value to its bioactive composition.The main component of EVOO is monounsaturated fatty acids(MUFAs)in the form of oleic acid.Oleic acid accounts for up to 70%-80%of EVOO.Secondly,EVOO contains approximately more than 30 phenolic compounds,of which HT is essential for the protection against cardiovascular diseases.In this review,we focused on the potential mechanisms of oleic acid and polyphenols combat cardiovascular diseases risk in terms of oxidative stress,inflammation,blood pressure,endothelial function and cholesterol.This review might provide a reference for the studies on cardiovascular protective effects of EVOO. | Yan Lu Jun Zhao Qiqi Xin Rong Yuan Yu Miao Manli Yang Hui Mo Keji Chen Weihong Cong | 2024 | Food Science and Human Wellness2024，13，2： | 0 |
| 7 | AC vector magnetometer for space-based applications using low-resource magneto-impedance sensor显示文摘This study proposes a novel AC vector magnetometer developed using a low-resource magneto-impedance sensor for China’s Feng-Yun meteorological satellite(FY-3E).It was calibrated and characterized to determine its performance parameters.The total weight of the AC vector magnetometer is 51 g(the aluminum box excluded),while the total power consumption is 310 m W.The proposed AC vector magnetometer can detect magnetic field variations in the range of±1000 nT and noise power spectral density of≤50 pT/Hz^(1/2)@1 Hz.Furthermore,the proposed device has a maximum nonlinearity of≤0.71‰over the entire range and a nonorthogonality error of 3.07 nT or 0.15%(root mean square).The total dose hardness of the sensor is≥30 krad(Si).Furthermore,we propose the first survey results of a magnetometer equipped aboard a Chinese FY-3E satellite in a Sunsynchronous orbit.The data revealed that the AC vector magnetometer can detect transient physical signals such as quasistatic field-aligned currents(~50 nT)and waves at the auroral latitudes.These features render the proposed AC vector magnetometer suitable for space-based applications,particularly those involving the study of geomagnetic activity. | YU XiangQian HUANG Cong XIAO ChiJie LI JiaWei LIU Si WANG JingDong LI YunPeng QU YaNan WANG YongFu CHEN HongFei ZOU Hong SHI WeiHong ZONG QiuGang CHEN XiaoFei ZHANG XiaoXin ZONG WeiGuo WANG JingSong | 2023 | Science China(Technological Sciences)2023，66，12： | 0 |
| 8 | Tetramethylpyrazine and paeoniflorin combination(TMP-PF)inhibits angiogenesis in atherosclerosis via miR-126/VEGF/VEGFR2 signaling pathway显示文摘Angiogenesis in atherosclerosis(AS)promotes plaque destabilization.miR-126 has a significant role in angiogenesis.Tetramethylpyrazine(TMP)and paeoniflorin(PF)have anti-atherosclerotic effects.However,the miR-126-related mechanisms of TMP and PF combination(TMP-PF)on angiogenesis in AS have not been understood.To explore the mechanism of TMP-PF on angiogenesis in AS targeting miR-126.Human umbilical vein endothelial cells(HUVECs)were assigned into the control,model,TMP-PF,TMP-PF+miR-126 inhibitor,and simvastatin groups.HUVECs were transfected with miR-126 inhibitor or negative control,incubated with oxidized low-density lipoprotein(ox-LDL)to establish AS model,and then treated with TMP-PF or simvastatin.Cell proliferation,migration,and tube formation assays are conducted,and the expression of angiogenesis-related factors were detected by enzyme-linked immunosorbent assay(ELISA)and Western blotting.The expression level of miR-126 was confirmed by polymerase chain reaction(PCR).0x-LDL promoted HUVECs proliferation,migration,and tube formation,downregulated miR-126 expression,and increased the expression of VEGF,VEGFR2,bFGF,and FGFR1.TMP-PF inhibited proliferation,migration,and tube formation,upregulated miR-126 expression and decreased the expression of VEGF,VEGFR2,bFGF,and FGFR1 in ox-LDL-induced HUVECs.However,the effects of TMP-PF on angiogenesis and the expression of miR-126,VEGF,VEGFR2,and FGFR1 were abolished by miR-126 inhibitor.TMP-PF suppressed angiogenesis in AS by regulating miR-126/VEGF/VEGFR2 pathway,which might elucidate the underlying mechanism of TMP-PF in alleviating AS. | Yahui Yuan Rong Yuan Qiqi Xin Yu Miao Ying Chen Rui Gao Weihong Cong | 2024 | Journal of Future Foods2024，4，3： | 0 |
| 9 | Expansion and adaptive evolution of the WRKY transcription factor family in Avicennia mangrove trees显示文摘Mangroves are adapted to intertidal zones,which present extreme environmental conditions.WRKYs are among the most prominent transcription factors(TFs)in higher plants and act through various interconnected networks to regulate responses to multiple abiotic stressors.Here,based on omic data,we investigated the landscape and evolutionary patterns of WRKYs in the main mangrove genus Avicennia.We found that both the number and the proportion of TFs and WRKYs in Avicennia species exceeded their inland relatives,indicating a signifcant expansion of WRKYs in Avicennia.We identifed 109 WRKY genes in the representative species Avicennia marina.Comparative genomic analysis showed that two recent wholegenome duplication(WGD)events played a critical role in the expansion of WRKYs,and 88%of Avicennia marina WRKYs(AmWRKYs)have been retained following these WGDs.Applying comparative transcriptomics on roots under experimental salt gradients,we inferred that there is high divergence in the expression of WGD-retained AmWRKYs.Moreover,we found that the expression of 16 AmWRKYs was stable between freshwater and moderately saline water but increased when the trees were exposed to high salinity.In particular,14 duplicates were retained following the two recent WGD events,indicating potential neo-and sub-functionalization.We also found that WRKYs could interact with other upregulated genes involved in signalling pathways and natural antioxidant biosynthesis to enhance salt tolerance,contributing to the adaptation to intertidal zones.Our omic data of the WRKY family in A.marina broadens the understanding of how a TF family relates to the adaptive evolution of mangroves. | Xiao Feng Guohong Li Weihong Wu Haomin Lyu Jiexin Wang Cong Liu Cairong Zhong Suhua Shi Ziwen He | 2023 | Marine Life Science & Technology2023，5，2： | 0 |
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