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| 1 | Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro显示文摘Dear Editor,In December 2019,a novel pneumonia caused by a previously unknown pathogen emerged in Wuhan,a city of 11 million people in central China.The initial cases were linked to exposures in a seafood market in Wuhan.1 As of January 27,2020,the Chinese authorities reported 2835 confirmed cases in China's Mainland,including 81 deaths.Additionally,19 confirmed cases were identified in Hong Kong,Macao and Taiwan,and 39 imported cases were identified in Thailand,Japan,South Korea,United States,Vietnam,Singapore,Nepal,France,Australia and Canada.The pathogen was soon identified as a novel coronavirus(2019-nCoV),which is closely related to sever acute respiratory syndrome CoV(SARS-CoV).2 Currently,there is no specific treatment against the new virus.Therefore,identifying effective antiviral agents to combat the disease is urgently needed. | Manli Wang Ruiyuan Cao Leike Zhang Xinglou Yang Jia Liu Mingyue Xu Zhengli Shi Zhihong Hu Wu Zhong Gengfu Xiao | 2020 | Cell Research2020，30，3： | 563 |
| 2 | Fe_3O_4@C nanoparticles as high-performance Fenton-like catalyst for dye decoloration显示文摘Water pollution has become serious environmental problem nowadays. Advanced oxidation processes(AOP) have been widely applied in water treatment.However, traditional Fenton reaction based on Fe2﹢-H2O2 system has obvious drawbacks, which limit its applications In this study, magnetic Fe3O4core-C shell nanoparticles(Fe3O4@C NPs) were prepared for the decoloration of methylene blue(MB) via the co-precipitation followed by the hydrothermal dehydrogenation of glucose. Fe3O4@C NPs showed high catalytic activity of the decoloration of MB through the decomposition of H2O2 in Fenton-like reactions. Fe3O4@C NPs had much higher activity than bare Fe3O4 cores, suggesting the coating of carbon enhanced the catalytic activity. The performance of Fe3O4@C NPs was better at lower pH and higher temperature, but was significantly inhibited in the presence of radical scavenger tertiary butanol. Fe3O4@C NPs could be magnetic separated and regenerated, and maintained with very good catalytic activity. The implication for the applications of Fe3O4@C NP-catalyzed Fenton-like reactions in water treatment was discussed. | Xiaoliang Zhang Manli He Jia-Hui Liu Rong Liao Lianqin Zhao Jingru Xie Ruijue Wang Sheng-Tao Yang Haifang Wang Yuanfang Liu | 2014 | Chinese Science Bulletin2014，59，27： | 8 |
| 3 | Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization显示文摘Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization (M-FISH) for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermedi- ate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chro- mosomes in the whole genome of human tumors. | Yiling Yang Jiayou Chu Yupeng Wu Manli Luo Xin Xu Yaling Han Yan Cai Qimin Zhan Mingrong Wang | 2008 | Journal of Genetics and Genomics2008，35，1： | 6 |
| 4 | Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres显示文摘The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide(PLGA) microsphere using synchrotron radiation–based Fourier-transform infrared spectromicroscopy(SR-FTIR).The representative infrared wavenumbers specific for protein/peptide(Exenatide) and excipient(PLGA) were identified and chemical maps at the single microsphere level were generated by measuring and plotting the intensity of these specific bands.For quantitative analysis of the distribution within microspheres,Matlab software was used to transform the map file into a 3D matrix and the matrix values specific for the drug and excipient were extracted.Comparison of the normalized SR-FTIR maps of PLGA and Exenatide indicated that PLGA was uniformly distributed,while Exenatide was relatively non-uniformly distributed in the microspheres.In conclusion,SR-FTIR is a rapid,nondestructive and sensitive detection technology to provide the distribution of chemical constituents and functional groups in microparticles and microspheres. | Manli Wang Xiaolong Lu Xianzhen Yin Yajun Tong Weiwei Peng Li Wu Haiyan Li Yan Yang Jingkai Gu Tiqiao Xiao Min Chen Jiwen Zhang | 2015 | Acta Pharmaceutica Sinica B2015，5，3： | 5 |
| 5 | Inhibition of cyclooxygenase-2 activity in subchondral bone modifies a subtype of osteoarthritis显示文摘Osteoarthritis(OA) causes the destruction of joints. Its pathogenesis is still under investigation, and there is no effective diseasemodifying therapy. Here, we report that elevated cyclooxygenase-2(COX-2) expression in the osteocytes of subchondral bone causes both spontaneous OA and rheumatoid arthritis(RA). The knockout of COX-2 in osteocytes or treatment with a COX-2 inhibitor effectively rescues the structure of subchondral bone and attenuates cartilage degeneration in spontaneous OA(STR/Ort)mice and tumor necrosis factor-α transgenic RA mice. Thus, elevated COX-2 expression in subchondral bone induces both OAassociated and RA-associated joint cartilage degeneration. The inhibition of COX-2 expression can potentially modify joint destruction in patients with arthritis. | Manli Tu Mi Yang Nanxi Yu Gehua Zhen Mei Wan Wenlong Liu Baochao Ji Hairong Ma Qiaoyue Guo Peijian Tong Li Cao Xianghang Luo Xu Cao | 2019 | Bone Research2019，7，3： | 5 |
| 6 | Bamboo charcoal enhances cellulase and urease activities during chicken manure composting:Roles of the bacterial community and metabolic functions显示文摘Microbial enzymes are crucial for material biotransformation during the composting process.In this study,we investigated the effects of adding bamboo charcoal(BC)(i.e.,at 5%,10%,and 20%corresponding to BC5,BC10,and BC20,respectively)on the enzyme activity levels during chicken manure composting.The results showed that BC10 could increase the cellulose and urease activities by 56%and 96%,respectively.The bacterial community structure in BC10 differed from those in the other treatments,and Luteivirga,Lactobacillus,Paenalcaligenes,Ulvibacter,Bacillus,Facklamia,Pelagibacterium,Sporosarcina,Cellvibrio,and Corynebacterium had the most important roles in composting.Compared with other treatments,BC10 significantly enhanced the average rates of degradation of carbohydrates(Dxylose(40%)andα-D-lactose(44%))and amino acids(L-arginine(16%),L-asparagine(14%),and L-threonine(52%)).We also explored the associations among the bacterial community and their metabolic functions with the changes in the activities of enzymes.Network analysis demonstrated that BC10 altered the co-occurrence patterns of the bacterial communities,where Ulvibacter and class Bacilli were the keystone bacterial taxa with high capacities for degrading carbon source,and they were related to increases in the activities of cellulase and urease,respectively.The results obtained in this study may help to further enhance the efficiency of composting. | Yanan Yin Chao Yang Jingrui Tang Jie Gu Haichao Li Manli Duan Xiaochang Wang Rong Chen | 2021 | Journal of Environmental Sciences2021，33，10： | 4 |
| 7 | Novel chemical permeation enhancers for transdermal drug delivery显示文摘Transdermal drug delivery has been accepted as a potential non-invasive route of drug administration,with advantages of prolonged therapeutic action,decreased side effect,easy use and better patient compliance.However,development of transdermal products is primarily hindered by the low permeability of the skin.To overcome this barrier effect,numerous new chemicals have been synthesized as potential permeation enhancers for transdermal drug delivery.In this review,we presented an overview of the investigations in this field,and further implications on selection or design of suitable permeation enhancers for transdermal drug delivery were also discussed. | Yang Chen Peng Quan Xiaochang Liu Manli Wang Liang Fang | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，2： | 3 |
| 8 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 9 | An initial-boundary value problem for the Maxwell equations显示文摘 | YANG PIWEN YANG SOU LI MANLI | 2010 | J Differential Equations2010，249，： | 1 |
| 10 | An initial–boundary value problem for the Maxwell equations显示文摘 | Piwen Yang Shuo Yang Manli Li | 2010 | Journal of Differential Equations2010，，12： | 1 |
| 11 | Numerical simulation of the behaviors of test square for prehistoric earthen sites during archaeological excavation显示文摘During the process of archaeological excavation in the regions of Southeast China, collapse of test square usually occurs due to poor site-specific conditions. In this paper, the fast Lagrangian analysis of continua in three dimensions(FLAC3D) is employed to reveal the behaviors of test square. Taking the archaeological works in Liangzhu prehistoric earthen sites as the research background, the paper first introduces the geological setting, excavation procedure and monitoring scheme of the studied test square. Then, the deformation of four sides of the test square is modeled using FLAC3D. By comparison, it shows that the numerical results are consistent with the results from field monitoring. The result suggests that the numerical simulation can be effectively applied to representing the actual behaviors of the test square,which is helpful for determination of excavation scheme and stability evaluation of the test square during archaeological excavation. | Xudong Wang Zongren Yu Jingke Zhang Qinglin Guo Shanlong Yang Manli Sun | 2018 | Journal of Rock Mechanics and Geotechnical Engineering2018，10，3： | 1 |
| 12 | The Wound-Inducible Lls1 Gene from Maize is an Orthologue of the Arabidopsis Acd1 Gene, and the LLS1 Protein is Present in Non-Photosynthetic Tissues显示文摘 | Manli Yang Ellen Wardzala Gurmukh S. Johal John Gray | 2004 | Plant Molecular Biology2004，，2： | 1 |
| 13 | Temperature and refractive index sensing characteristics of an MZI-based multimode fiber–dispersion compensation fiber–multimode fiber structure显示文摘 | Hao Sun Shen Yang Jing Zhang Qiangzhou Rong Lei Liang Qinfang Xu Guanghua Xiang Dingyi Feng Yanying Du Zhongyao Feng Xueguang Qiao Manli Hu | 2012 | Optical Fiber Technology2012，，6： | 1 |
| 14 | Activation of Th17/Th1 and Th1, but not Th17, is associated with the acute cardiac event in patients with acute coronary syndrome显示文摘 | Zhao Zhao Yue Wu Manli Cheng Yuqiang Ji Xiaobo Yang Ping Liu Shaobin Jia Zuyi Yuan | 2011 | Atherosclerosis2011，，2： | 1 |
| 15 | Construction of Ad-EGFP-BDNF vector and its expression in neural stem cells显示文摘BACKGROUND:Brain-derived neurotrophic factor(BDNF) provides nourishment to injured neurons.Neural stem cells can differentiate into neurons to repair neuronal injury in vivo.It has been hypothesized that continuous secretion of BDNF from neural stem cells could benefit brain injury repair.OBJECTIVE:To transfect BDNF and enhanced green fluorescent protein(EGFP) into neural stem cells with adenovirus vector and to observe expression of BDNF and EGFP in transfected neural stem cells.DESIGN,TIME AND SETTING:Observational,cellular,molecular study was performed at the Biochemistry Laboratory,Tongji University School of Medicine,China from July 2004 to September 2006.MATERIALS:Neural stem cells were provided by the Anatomy and Histoembryology Laboratory of Fudan University Medical School,China.METHODS:BDNF cDNA was extracted by reverse transcription polymerase chain reaction from the rat hippocampus.Following gene cloning and packaging by HEK293.BDNF,the EGFP gene was transfected into cultured neural stem cells with the Ad-EGFP-BDNF vector.BDNF-expressing neural stem cell clones were selected by G418 selection.MAIN OUTCOME MEASURES:EGFP expression and cell morphology were observed by fluorescent microscopy;neural stem cell expressing BDNF mRNA was examined by reverse transcription polymerase chain reaction;BDNF expression was detected by enzyme-linked immunosorbent assay from supernatant of infected neural stem cells.RESULTS:High transfection efficiency was obtained using 5 × 108 virus titers to transfect neural stem cells.G418-resistant neural stem cell clones integrated BDNF mRNA fragments.Enzyme-linked immunosorbent assay results showed that BDNF expression in the supernatant increased with increasing culture time and peaked at 72 hours.CONCLUSION:Adenovirus-mediated BDNF and EGFP genes were successfully transfected into neural stem cells and were expressed in neural stem cells for a long period of time. | Shaohua Li Zhenhua Li Huilong Wen Xin Gu Yinjiang Cui Yang Wang Lixia Lu Yanna Li Manli Na | 2010 | Neural Regeneration Research2010，5，13： | 1 |
| 16 | Simultaneous measurement of temperature and strain or temperature and curvature based on an optical fiber Mach–Zehnder interferometer显示文摘 | Hao Sun Shen Yang Xiaolei Zhang Liutong Yuan Zaihang Yang Manli Hu | 2014 | Optics Communications2014，，： | 1 |
| 17 | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China显示文摘Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV. | Dahe Yang Jun Wang Xi Wang Fei Deng Qingyun Diao Manli Wang Zhihong Hu Chunsheng Hou | 2022 | Virologica Sinica2022，37，4： | 1 |
| 18 | The novel peptide Nb PPI1 identified from Nicotiana benthamiana triggers immune responses and enhances resistance against Phytophthora pathogens显示文摘In plants, recognition of small secreted peptides, such as damage/danger-associated molecular patterns(DAMPs), regulates diverse processes,includingstressandimmune responses. Here, we identified an SGPS(Ser-Gly-Pro-Ser) motif-containing peptide, Nicotiana tabacum NtPROPPI, and its two homologs in Nicotiana benthamiana, NbPROPPI1 and NbPROPPI2. Phytophthora parasitica infection and salicylic acid(SA) treatment induced NbPROPPI1/2 expression. Moreover, SignalP predicted that the 89-amino acid NtPROPPI includes a 24-amino acid N-terminal signal peptide and NbPROPPI1/2-GFP fusion proteins were mainly localized to the periplasm. Transient expression of NbPROPPI1/2 inhibited P.parasiticacolonization,andNbPROPPI1/2 knockdown rendered plants more susceptible to P. parasitica. An eight-amino-acid segment in the NbPROPPI1 C-terminus was essential for its immune function and a synthetic 20-residue peptide, NbPPI1, derived from the C-terminus of NbPROPPI1 provoked significant immune responses in N. benthamiana. These responses led to enhanced accumulation of reactive oxygen species, activation of mitogen-activated protein kinases, and up-regulation of the defense genes Flg22-induced receptor-like kinase(FRK) and WRKY DNA-binding protein 33(WRKY33). The NbPPI1-induced defense responses require Brassinosteroid insensitive1-associated receptor kinase 1(BAK1). These results suggest that NbPPI1 functions as a DAMP in N. benthamiana;this novel DAMP provides a potentially useful target for improving plant resistance to Pytophthora pathogens. | Qujiang Wen Manli Sun Xianglan Kong Yang Yang Qiang Zhang Guiyan Huang Wenqin Lu Wanyue Li Yuling Meng Weixing Shan | 2021 | Journal of Integrative Plant Biology2021，63，5： | 1 |
| 19 | Comparative Studies on Preparation of Large Plant DNA Fragments by Pulse Field Gel Electrophoresis显示文摘The preparation of large plant DNA fragments is extremely important to the construction of large insert DNA libraries (YAC, BAC, PAC and TAC). Although several techniques have been developed in each step of large plant DNA fragments preparation, the whole processing remains complicated and difficult. Based on authors research experience and the recent worldwide development in this field, the following aspects are discussed in this paper: techniques of plant high molecular weight (HMW) DNA purification by pre-electrophoresis, the optimal conditions for the partial digestion of the HMW DNA by HindIII, the isolation effects of of large plant DNA fragments (100~400 kb) with different parameters of pulse field gel electrophoresis (PFGE), and the recovery of large DNA fragments. Through comparative studies, the advantages and disadvantages of each technique are discussed and some recommendations are proposed for preparing high quality large plant DNA fragments. The suggested techniques have been used in preparing the large DNA fragments of maize, rice, moss, laver, sea tangle and peach,and similar results are obtained among all the materials. This paper only reports the results using maize as material. | 杨继良 王庆华 Deng Daiyong Yang Dianer JIN Demin WENG Manli ZHANG Juren Wang Bin | 2004 | High Technology Letters2004，10，1： | 1 |
| 20 | Meta-Analysis: The Efficacy and Safety of Paricalcitol for the Treatment of Secondary Hyperparathyroidism and Proteinuria in Chronic Kidney Disease显示文摘 | Tianzhao Han Gong Rong Dayong Quan Ying Shu Zhu Liang Ninglan She Manli Liu Bing Yang Gong Cheng Yongman Lv Leonard Stern Sihe Wang | 2012 | BioMed Research International2012，，： | 1 |
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