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| 1 | Studies of Primary Angle Qosure Glaucoma in China显示文摘The resent researches on primary angle closure glaucoma (PACG) in China were reviewed. The prevalence of PACG (0.41%) in chinese is between the Caucasian and Eskimos, and chronic type is the dominant type in PACG. However, with the economic development and medical care improvements, the prevalence condition of PACG may change; several researches discovered that the mechanism of PACG, especially the chronic type, is not only because of pupil-blocking but also closely related to the mechanism of crowding of peripheral iris and the anterior location of the ciliary body. We supposed that PACG can be divided into several subtypes based on this machnism. Also, we believe that the use of ultrasound biomicroscopy (UBM) and ophthalmic laser in China has promoted the research of PACG and improved the level of prevention and treatment of PACG. Eye Science 1997; 13 : 120- 124. | Ningli Wang, Wenbin Zhou, Tiancai Ye, Qiang Yu, Mingying Lai, Meihua LeZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China | 1997 | 眼科学报1997，13，3： | 7 |
| 2 | Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/ Cas9-mediated knock-in显示文摘In mammalian cells,long noncoding RNAs(lncRNAs)form complexes with proteins to execute various biological functions such as gene transcription,RNA processing and other signaling activities.However,methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interacting proteins are limited.Here,we report the development of CERTIS(CRISPR-mediated Endogenous lncRNA Tracking and Immunoprecipitation System)to visualize and isolate endogenous lncRNA,by precisely inserting a 24-repeat MS2 tag into the distal end of lncRNA locus through the CRISPR7Cas9 technology.In this study,we show that CERTIS effectively labeled the paraspeckle lncRNA NEAT1 without disturbing its physiological properties and could monitor the endogenous expression variation of NEAT1.In addition,CERTIS displayed superior performance on both short-and long-term tracking of NEAT1 dynamics in live cells.We found that NEAT1 and paraspeckles were sensitive to topoisomerase I specific inhibitors.Moreover,RNA Immunoprecipitation(RIP)of the MS2-tagged NEAT1 lncRNA successfully revealed several new protein components of paraspeckle.Our results support CERTIS as a tool suitable to track both spatial and temporal lncRNA regulation in live cells as well as study the lncRNA-protein interactomes. | Bohong Chen Shengcheng Deng Tianyu Ge Miaoman Ye Jianping Yu Song Lin Wenbin Ma Zhou Songyang | 2020 | Protein & Cell2020，11，9： | 4 |
| 3 | Diagnosis and management of acquired thrombotic thrombocy- topenic purpura in southeast China: a single center experience of 60 cases显示文摘 | Xinping Zhou XingnongYe Yanling Ren Chen Mei Liya Ma Jiansong Huang Weilai Xu Juying Wei Li Ye Wenyuan Mai Wenbin Qian Haitao Meng Jie Jin Hongyan Tong | 2016 | Frontiers of Medicine2016，10，4： | 2 |
| 4 | Effect of particle size distribution on the hydration and compressive strength development ofα-calcium sulfate hemihydrate paste显示文摘 | YE Qingqing GUAN Baohong LOU Wenbin | 2011 | Power Technology2011，207，： | 1 |
| 5 | The Solar Upper Transition Region Imager(SUTRI)Onboard the SATech-01 Satellite显示文摘The Solar Upper Transition Region Imager(SUTRI)onboard the Space Advanced Technology demonstration satellite(SATech-01),which was launched to a Sun-synchronous orbit at a height of~500 km in 2022 July,aims to test the on-orbit performance of our newly developed Sc/Si multi-layer reflecting mirror and the 2k×2k EUV CMOS imaging camera and to take full-disk solar images at the Ne VII 46.5 nm spectral line with a filter width of~3 nm.SUTRI employs a Ritchey-Chrétien optical system with an aperture of 18 cm.The on-orbit observations show that SUTRI images have a field of view of~416×416 and a moderate spatial resolution of~8″without an image stabilization system.The normal cadence of SUTRI images is 30 s and the solar observation time is about16 hr each day because the earth eclipse time accounts for about 1/3 of SATech-01's orbit period.Approximately15 GB data is acquired each day and made available online after processing.SUTRI images are valuable as the Ne VII 46.5 nm line is formed at a temperature regime of~0.5 MK in the solar atmosphere,which has rarely been sampled by existing solar imagers.SUTRI observations will establish connections between structures in the lower solar atmosphere and corona,and advance our understanding of various types of solar activity such as flares,filament eruptions,coronal jets and coronal mass ejections. | Xianyong Bai Hui Tian Yuanyong Deng Zhanshan Wang Jianfeng Yang Xiaofeng Zhang Yonghe Zhang Runze Qi Nange Wang Yang Gao Jun Yu Chunling He Zhengxiang Shen Lun Shen Song Guo Zhenyong Hou Kaifan Ji Xingzi Bi Wei Duan Xiao Yang Jiaben Lin Ziyao Hu Qian Song Zihao Yang Yajie Chen Weidong Qiao Wei Ge Fu Li Lei Jin Jiawei He Xiaobo Chen Xiaocheng Zhu Junwang He Qi Shi Liu Liu Jinsong Li Dongxiao Xu Rui Liu Taijie Li Zhenggong Feng Yamin Wang Chengcheng Fan Shuo Liu Sifan Guo Zheng Sun Yuchuan Wu Haiyu Li Qi Yang Yuyang Ye Weichen Gu Jiali Wu Zhe Zhang Yue Yu Zeyi Ye Pengfeng Sheng Yifan Wang Wenbin Li Qiushi Huang Zhong Zhang | 2023 | Research in Astronomy and Astrophysics2023，23，6： | 1 |
| 6 | Interaction between α-calcium sulfate hemihydrate and superplasticizer from the point of adsorption characteristics, hydration and hardening process显示文摘 | Baohong Guan Qingqing Ye Jiali Zhang Wenbin Lou Zhongbiao Wu | 2009 | Cement and Concrete Research2009，，2： | 1 |
| 7 | Boundary value problem for a coupled system of fractional differential equations with p-Laplacian operator at resonance显示文摘 | Cheng Lingling Liu Wenbin Ye Qingqing | 2014 | Electron J Diff Equ2014，60，： | 1 |
| 8 | Experiment studies of bottom influence in shallow layer water explosion显示文摘 | Gu Wenbin Ye Xushuang Zhang Pengxiang | 2001 | Journal of PLA University of Science and Technology2001，2，2： | 1 |
| 9 | Preoperative chemoradiotherapy for low rectal cancer, protecting anal surgery clinical study 显示文摘 | gandofyan Ye Tao Yao Zeming Shen Wenbin | 2009 | Journal of xuzhou medical college2009，，10： | 1 |
| 10 | JaponiconeA induces apoptosis of bortezomib-sensitive and -resistant myeloma cells in vitro and in vivo by targeting IKKβ显示文摘Objective:Multiple myeloma(MM)remains incurable with high rates of relapse.New therapeutic drugs are therefore urgently needed to improve the prognosis.JaponiconeA(JA),a natural product isolated from Inula japonica Thunb,has shown good anti-MM potential.A comprehensive study should therefore be conducted to identify both the in vitro and in vivo mechanisms of the anti-MM effects of JA.Methods:CCK8 assays and flow cytometry were used to detect the proliferation,apoptosis,and cell cycle of MM cell lines when treated with JA.In vivo experiments were conducted using subcutaneous xenograft mouse models.We also identified possible targets and the mechanism of JA using RNA-seq and c-Map databases,and identified the specific targets of JA in bortezomib-sensitive and-resistant MM cell lines using CETSA,DARTS,and rescue experiments.Furthermore,JA and bortezomib were used separately or together to characterize their possible synergistic effects.Results:In vitro,JA inhibited proliferation,and induced apoptosis and G2/M phase arrest in MM cell lines,and selectively killed primary CD138+MM cells.In vivo,JA also demonstrated a strong anti-tumor effect with no observable toxicity.In addition,JA showed synergetic effects in combination with bortezomib,and enhanced the anti-tumor effect of bortezomib in bortezomibresistant cells.CETSA and DARTS confirmed direct binding of JA to NF-κB inhibitor kinase beta(IKKβ),and overexpression of IKKβor knockdown of IκBαpartially rescued the apoptosis induced by JA.Conclusions:JA exhibited strong anti-tumor effects in MM.It sensitized myeloma cells to bortezomib and overcame NF-κB-induced drug resistance by inhibiting IKKβ,providing a new treatment strategy for MM patients. | Zilu Zhang Chenjing Ye Jia Liu Wenbin Xu Chao Wu Qing Yu Xiaoguang Xu Xinyi Zeng Huizi Jin Yingli Wu Hua Yan | 2022 | Cancer Biology & Medicine2022，19，5： | 1 |
| 11 | Comparison of the thermal properties between composites reinforced by raw and amino-functionalized carbon materials 显示文摘 | Hu Yizhe Shen Jianfeng Li Na Hongwei Ma Minshi Yan Bo Huang Weishi Wang Wenbin Ye Mingxin | 2010 | Composite Science and Technology2010，70，15： | 1 |
| 12 | An adiabatic low-temperature calorimeter for heat capacity measurement of small samples显示文摘 | Zhicheng Tan Guanyu Sun Yi Sun Anxue Yin Wenbin Wang Jinchun Ye Lixing Zhou | 1995 | Journal of Thermal Analysis (-)1995，，1： | 1 |
| 13 | Effect of particle size distribution on the hydration and compressive strength development of α-calcium sulfate hemihydrate paste显示文摘 | Ye Qingqing Guan Baohong Lou Wenbin | | 0，，： | 1 |
| 14 | An adiabatic low-temperature calorimeter for heat capacity measurement of small samples显示文摘 | Zhicheng Tan Guanyu Sun Yi Sun Anxue Yin Wenbin Wang Jinchun Ye Lixing Zhou | 1995 | Journal of Thermal Analysis (-)1995，，1： | 1 |
| 15 | Identification and phylogenetic analysis of two canine coronavirus strains显示文摘Canine coronavirus(CCoV),a member of the genus Alphocoronavirus,is an enveloped,single-stranded positive-sense RNA virus that responsible for gastroenteritis in dogs.In this study,two CCoV isolates were successfully propagated from 53 CCoV-positive clinical specimens by serial passaging in A-72 cells.These two strains,CCoV JS1706 and CCoV JS1712,caused cytopathic effects in A-72 cells.The sizes of virus plaque formed by them differed in early passages.Electron microscopy revealed a large quantity of typical coronavirus particles with 80-120 nm in diameter in cell culture media and cytoplasm of infected cells,in which they appeared as inclusion bodies.RT-PCR analysis of 5 gene indicated that these two isolates were belonged to CCoV lla subtype.Homology of RdRp,S,M and N proteins between the two strains were 100,99.6,99.2 and 100.0%,respectively,whereas they were 99.4-100%,83.1-95.2%,88.5-99.2% and 91.9-99.7%identity compared to CCoV II reference strains.Phylogenetic analysis of RdRp,S,M and N protein showed that they were closely related to CCoV II strains.These two subtype lla isolates will be useful for evaluating the pathogenesis and evolution of CCoV and for developing diagnostic reagents and vaccines. | Junji Gan Ye Tang Haifeng Lv Wenbin Xiong Xiaoyan Tian | 2021 | Animal Diseases2021，1，2： | 1 |
| 16 | Multi-chain Fudan-CCDC model for COVID-19—a revisit to Singapore's case显示文摘Background:COVID-19 has been impacting on the whole world critically and constantly since late December 2019.Rapidly increasing infections has raised intense worldwide attention.How to model the evolution of COVID-19 effectively and efficiently is of great significance for prevention and control.Methods:We propose the multi-chain Fudan-CCDC model based on the original single-chain model in[Shao et al.2020]to describe the evolution of COVID-19 in Singapore.Multi-chains can be considered as the superposition of several single chains with different characteristics.We identify the parameters of models by minimizing the penalty function.Results:The numerical simulation results exhibit the multi-chain model performs well on data fitting.Though unsteady the increments are,they could still fall within the range of±30%fluctuation from simulation results.Conclusion:The multi-chain Fudan-CCDC model provides an effective way to early detect the appearance of imported infectors and super spreaders and forecast a second outbreak.It can also explain the data from those countries where the single-chain model shows deviation from the data. | Hanshuang Pan Nian Shao Yue Yan Xinyue Luo Shufen Wang Ling Ye Jin Cheng Wenbin Chen | 2020 | Quantitative Biology2020，8，4： | 1 |
| 17 | A Study of Ore-forming Fluids in the Shimensi Tungsten Deposit,Dahutang Tungsten Polymetallic Ore Field,Jiangxi Province,China显示文摘The Dahutang tungsten polymetallic ore field is located north of the Nanling W-Sn polymetallic metallogenic belt and south of the Middle—Lower Yangtze River Valley Cu-Mo-Au-Fe porphyry-skarn belt.It is a newly discovered ore field,and probably represents the largest tungsten mineralization district in the world.The Shimensi deposit is one of the mineral deposits in the Dahutang ore field,and is associated with Yanshanian granites intruding into a Neoproterozoic granodiorite batholith.On the basis of geologic studies,this paper presents new petrographic,microthermometric,laser Raman spectroscopic and hydrogen and oxygen isotopic studies of fluid inclusions from the Shimensi deposit.The results show that there are three types of fluid inclusions in quartz from various mineralization stages:liquid-rich two-phase fluid inclusions,vapor-rich two-phase fluid inclusions,and three-phase fluid inclusions containing a solid crystal,with the vast majority being liquid-rich two-phase fluid inclusions.In addition,melt and melt-fluid inclusions were also found in quartz from pegmatoid bodies in the margin of the Yanshanian intrusion.The homogenization temperatures of liquid-rich two-phase fluid inclusions in quartz range from 162 to 363℃ and salinities are 0.5wt%-9.5wt%NaCI equivalent.From the early to late mineralization stages,with the decreasing of the homogenization temperature,the salinity also shows a decreasing trend.The ore-forming fluids can be approximated by a NaCl-H_2O fluid system,with small amounts of volatile components including CO_2,CH_4 and N_2,as suggested by Laser Raman spectroscopic analyses.The hydrogen and oxygen isotope data show that δ5D_(V-smow) values of bulk fluid inclusions in quartz from various mineralization stages vary from-63.8‰ to-108.4‰,and the δ^(18)O_(H2O) values calculated from the δ^(18)O_(V-)smow values of quartz vary from-2.28‰ to 7.21‰.These H-O isotopic data are interpreted to indicate that the ore-forming fluids are mainly composed of magmatic water in the early stage,and meteoric water was added and participated in mineralization in the late stage.Integrating the geological characteristics and analytical data,we propose that the ore-forming fluids of the Shimensi deposit were mainly derived from Yanshanian granitic magma,the evolution of which resulted in highly differentiated melt,as recorded by melt and melt-fluid inclusions in pegmatoid quartz,and high concentrations of metals in the fluids.Cooling of the ore-forming fluids and mixing with meteoric water may be the key factors that led to mineralization in the Dahutang tungsten polymetallic ore field. | GONG Xiaodong YAN Guangsheng YE Tianzhu ZHU Xinyou LI Yongsheng ZHANG Zhihui JIA Wenbin YAO Xiaofeng | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 1 |
| 18 | Two-dimensional material-based virus detection显示文摘Cost-effective, rapid, and accurate virus detection technologies play key roles in reducing viral transmission. Prompt and accurate virus detection enables timely treatment and effective quarantine of virus carrier, and therefore effectively reduces the possibility of large-scale spread. However, conventional virus detection techniques often suffer from slow response, high cost or sophisticated procedures. Recently, two-dimensional(2D) materials have been used as promising sensing platforms for the highperformance detection of a variety of chemical and biological substances. The unique properties of 2D materials, such as large specific area, active surface interaction with biomolecules and facile surface functionalization, provide advantages in developing novel virus detection technologies with fast response and high sensitivity. Furthermore, 2D materials possess versatile and tunable electronic, electrochemical and optical properties, making them ideal platforms to demonstrate conceptual sensing techniques and explore complex sensing mechanisms in next-generation biosensors. In this review, we first briefly summarize the virus detection techniques with an emphasis on the current efforts in fighting again COVID-19. Then, we introduce the preparation methods and properties of 2D materials utilized in biosensors, including graphene, transition metal dichalcogenides(TMDs) and other 2D materials. Furthermore, we discuss the working principles of various virus detection technologies based on emerging 2D materials, such as field-effect transistor-based virus detection, electrochemical virus detection, optical virus detection and other virus detection techniques. Then, we elaborate on the essential works in 2D material-based high-performance virus detection. Finally, our perspective on the challenges and future research direction in this field is discussed. | Wenbin Wang Wei Zhai Ye Chen Qiyuan He Hua Zhang | 2022 | Science China Chemistry2022，65，3： | 1 |
| 19 | Hydrodearomatization Kinetics of Diesel Fraction and Catalyst Stacking Simulation显示文摘The kinetics of hydrodearomatization (HDA) is studied in an isothermal high-throughput reactor over three catalysts (CoMo, NiMo, and NiMoW) to produce clean diesel fuel according to China’s latest emission standards. The influences of reaction temperature, pressure, the ratio of H2 to oil, and space time were systematically investigated. By analyzing the reaction mechanism, a four-lump kinetic model considering the influence of competitive adsorption was proposed for the hydrodearomatization reaction, and the model parameters were optimized with good fitting. It was found that nitrogen compounds inhibited the hydrodearomatization reaction. The simulation of various catalyst stacking schemes based on the HDA kinetic model is close to the experimental data, proving the reliability of the model. The concentration of aromatic compounds of different loading sequences was predicted with the catalyst gradation model. | Wei Ye Sun Xinge LüHailong Jiang Hongbo Chen Wenbin Qin Kang Li Mingfeng Nie Hong | 2022 | China Petroleum Processing & Petrochemical Technology2022，24，3： | 0 |
| 20 | A Survey on Methods for Predicting Polyadenylation Sites from DNA Sequences,Bulk RNA-seq,and Single-cell RNA-seq显示文摘Alternative polyadenylation(APA)plays important roles in modulating mRNA stability,translation,and subcellular localization,and contributes extensively to shaping eukaryotic transcriptome complexity and proteome diversity.Identification of poly(A)sites(pAs)on a genomewide scale is a critical step toward understanding the underlying mechanism of APA-mediated gene regulation.A number of established computational tools have been proposed to predict pAs from diverse genomic data.Here we provided an exhaustive overview of computational approaches for predicting pAs from DNA sequences,bulk RNA sequencing(RNA-seq)data,and single-cell RNA sequencing(scRNA-seq)data.Particularly,we examined several representative tools using bulk RNA-seq and scRNA-seq data from peripheral blood mononuclear cells and put forward operable suggestions on how to assess the reliability of pAs predicted by different tools.We also proposed practical guidelines on choosing appropriate methods applicable to diverse scenarios.Moreover,we discussed in depth the challenges in improving the performance of pA prediction and benchmarking different methods.Additionally,we highlighted outstanding challenges and opportunities using new machine learning and integrative multi-omics techniques,and provided our perspective on how computational methodologies might evolve in the future for non-30 untranslated region,tissuespecific,cross-species,and single-cell pA prediction. | Wenbin Ye Qiwei Lian Congting Ye Xiaohui Wu | 2023 | Genomics, Proteomics & Bioinformatics2023，21，1： | 0 |
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