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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair显示文摘间充质的干细胞(MSC ) 为治疗各种各样的疾病有大潜力,特别那些与织物有关损坏包含有免疫力的反应。各种各样的研究证明了 MSC 在 vitro 并且在 vivo 是强烈抑制免疫力的。我们的最近的研究证明了那未刺激的 MSC 确实不能免疫力的抑制;他们与 TNF- 伪, IL-1 伪或 IL-1 尾与激活的淋巴细胞的上层清液,或与 IFN- 纬的联合在刺激之上变得 potently 抑制免疫力。这观察表明在某些情形下面,煽动性的 cytokines 能实际上变得抑制免疫力。我们证明在调停 MSC 的免疫力的抑制的机制有一个种类变化:由告知 cytokine 的老鼠 MSC 的免疫力的抑制被氮的氧化物调停(没有) 而由告知 cytokine 的人的 MSC 的免疫力的抑制通过 indoleamine 被执行 2, 3-dioxygenase (伊多语) 。在有煽动性的 cytokines 的刺激之上,另外,老鼠和人的 MSC 分泌几白血球 chemokines 显然服务与 MSC 吸引有免疫力的房间进最近,在哪儿不或伊多语被预言很活跃。因此,由煽动性的刺激 cytokine 的 MSC 的免疫力的抑制经由 chemokines 的一致行动发生并且免疫者禁止没有或伊多语由 MSC 生产了。因此,我们的结果在对待有免疫力的回答导致的织物损害关于调停 MSC 的免疫力的抑制并且为 MSC 的更好的申请的机制提供新奇信息。 | Yufang Shi Gangzheng Hu Juanjuan Su Wenzhao Li Qing Chen Peishun Shou Chunliang Xu Xiaodong Chen Yin Huang Zhexin Zhu Xin Huang Xiaoyan Han Ningxia Xie Guangwen Ren | 2010 | Cell Research2010，20，5： | 72 |
| 3 | Incidence trend of nasopharyngeal carcinoma from 1987 to 2011 in Sihui County,Guangdong Province,South China:an age-period-cohort analysis显示文摘Introduction:In the past several decades,declining incidences of nasopharyngeal carcinoma(NPC) have been observed in Chinese populations in Hong Kong,Taiwan,Los Angeles,and Singapore.A previous study indicated that the incidence of NPC in Sihui County,South China remained stable until 2002,but whether age,diagnosis period,and birth cohort affect the incidence of NPC remains unknown.Methods:Age-standardized rates(ASRs) of NPC incidence based on the world standard population were examined in both males and females in Sihui County from 1987 to 2011.Joinpoint regression analysis was conducted to quantify the changes in incidence trends.A Poisson regression age-period-cohort model was used to assess the effects of age,diagnosis period,and birth cohort on the risk of NPC.Results:The ASRs of NPC incidence during the study period were 30.29/100,000 for males and 13.09/100,000 for females.The incidence of NPC remained stable at a non-significant average annual percent change of 0.2%for males and-1.6%for females throughout the entire period.A significantly increased estimated annual percent change of 6.8%(95%confidence interval,0.1%-14.0%) was observed from 2003 to 2009 for males.The relative risk of NPC increased with advancing age up to 50-59 and decreased at ages >60 years.The period effect curves on NPC were nearly flat for males and females.The birth cohort effect curve for males showed an increase from the1922 cohort to the 1957 cohort and a decrease thereafter.In females,there was an undulating increase in the relative risk from the 1922 cohort to the 1972 cohort.Conclusions:The incidence trends for NPC remained generally stable in Sihui from 1987 to 2011,with an increase from 2003 to 2009.The relative risks of NPC increased in younger females. | Li-Fang Zhang Yan-Hua Li Shang-Hang Xie Wei Ling Sui-Hong Chen Qing Liu Qi-Hong Huang Su-Mei Cao | 2015 | Chinese Journal of Cancer2015，34，8： | 47 |
| 4 | Plasma exchange-centered artificial liver support system in hepatitis B virus-related acute-onchronic liver failure:a nationwide prospective multicenter study in China显示文摘BACKGROUND:Plasma exchange(PE)-centered artificial liver support system reduced the high mortality rate of hepatitis B virus(HBV)-related acute-on-chronic liver failure(ACLF).But the data were diverse in different medical centers.The present prospective nationwide study was to evaluate the effects of PE on patients with HBV-ACLF at different stages.METHODS:From December 2009 to December 2011,we evaluated 250 patients at different stages of HBV-ACLF from 10 major medical centers in China.All the laboratory parameters were collected at admission,before and after PE.RESULTS:Among the 250 patients who underwent 661 rounds of PE,one-month survival rate was 61.6%; 141(56.4%) showed improvement after PE.Variables such as age(P=0.000),levels of total bilirubin(TB,P=0.000),direct bilirubin(P=0.000),total triglycerides(P=0.000),low-density lipoprotein(P=0.022),Na+(P=0.014),Cl–(P=0.038),creatinine(Cr,P=0.007),fibrinogen(P=0.000),prothrombin time(PT,P=0.000),white blood cell(P=0.000),platelet(P=0.003) and MELD(P=0.000) were significantly related to prognosis.Multivariate logistic regression analysis showed that age,disease stage,TB,Cr and PT levels were independent risk factors of mortality among HBV-ACLF patients.CONCLUSIONS:PE can improve the clinical outcome of patients with HBV-ACLF.Levels of TB,Cr and PT,age and disease stage help to predict prognosis. | Jia-Jia Chen Jian-Rong Huang Qian Yang Xiao-Wei Xu Xiao-Li Liu Shao-Rui Hao Hui-Fen Wang Tao Han Jing Zhang Jian-He Gan Zhi-Liang Gao Yu-Ming Wang Shu-Mei Lin Qing Xie Chen Pan Lan-Juan Li | 2016 | Hepatobiliary & Pancreatic Diseases International2016，15，3： | 48 |
| 5 | Effect of hepatocyte apoptosis induced by TNF-α on acute severe hepatitis in mouse models显示文摘AIM To study the effect of hepatocyteapoptosis and necrosis induced by TNF-α on thepathogenesis of acute severe hepatitis(ASH).METHODS The model of ASH was prepared inD-galactosamine(GAIN)sensitized BALB/c miceby injection of either endotoxin(ET)or tumornecrosis factor-α(TNF-α).Morphologicalchanges of apoptotic hepatocytes were studiedby both light and electron microscope and in siteend labeling method(ISEL).Molecular biologicalchanges of DNA ladder were observed byelectrophoresis of extract from liver tissues.Biochemical changes were measured by alanineaminotransferase(ALT),asparticaminotransferase(AST)and TNF-α.The relationbetween apoptosis and necrosis was evaluatedsimultaneously.RESULTS The sequence of hepatocyteapoptosis,necrosis,and final death from ASHwas observed both in GAIN/ET and GAIN/TNF-agroup.Apoptosis was prominent at 3.5 h and 5 hafter injection of inducer,while necrosis becamedominant at 9 h after challenge.The appearanceof apoptosis was earlier in GAIN/TNF-α groupthan that in GAIN/ ET group.Pretreatment ofmice with antiTNF IgG1 may completely preventthe liver injury induced by GalN/ET.CONCLUSION TNF-α can cause liver damageby inducing hepatic apoptosis and necrosis inmice with endotoxemia. | Guo Qing Zang Xia Qiu Zhou Hong Yu Qing Xie Guo Ming Zhao Bin Wang Qing Guo Yue Qin Xiang Dan Liao Department of Infectious Diseases,Rujin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2000 | World Journal of Gastroenterology2000，6，5： | 30 |
| 6 | From concept to reality-A review to the primary test stand and its preliminary application in high energy density physics显示文摘Pulsed power technology,whereas the electrical energy stored in a relative long period is released in much shorter timescale,is an efficient method to create high energy density physics(HEDP)conditions in laboratory.Around the beginning of this century,China Academy of Engineering Physics(CAEP)began to build some experimental facilities for HEDP investigations,among which the Primary Test Stand(PTS),a multi-module pulsed power facility with a nominal current of 10 MA and a current rising time~90 ns,is an important achievement on the roadmap of the electro-magnetically driven inertial confinement fusion(ICF)researches.PTS is the first pulsed power facility beyond 10 TW in China.Therefore,all the technologies have to be demonstrated,and all the engineering issues have to be overcome.In this article,the research outline,key technologies and the preliminary HEDP experiments are reviewed.Prospects on HEDP research on PTS and pulsed power development for the next step are also discussed. | Jianjun Deng Weiping Xie Shuping Feng Meng Wang Hongtao Li Shengyi Song Minghe Xia Ji Ce An He Qing Tian Yuanchao Gu Yongchao Guan Bin Wei Xianbin Huang Xiaodong Ren Jiakun Dan Jing Li Shaotong Zhou Hongchun Cai Siqun Zhang Kunlun Wang Qiang Xu Yujuan Wang Zhaohui Zhang Guilin Wang Shuai Guo Yi He Yiwei Zhou Zhanji Zhang Libing Yang Wenkang Zou | 2016 | Matter and Radiation at Extremes2016，1，1： | 19 |
| 7 | Daclatasvir plus asunaprevir in treatment-na?ve patients with hepatitis C virus genotype 1b infection显示文摘AIM To assess daclatasvir plus asunaprevir(d UAL) in treatment-na?ve patients from China's Mainland, Russia and South Korea with hepatitis C virus(HCV) genotype 1 b infection. METHODS Patients were randomly assigned(3:1) to receive 24 wk of treatment with d UAL(daclatasvir 60 mg once daily and asunaprevir 100 mg twice daily) beginning on day 1 of the treatment period(immediate treatment arm) or following 12 wk of matching placebo(placebodeferred treatment arm). The primary endpoint was a comparison of sustained virologic response at posttreatment week 12(SVR12) compared with the historical SVR rate for peg-interferon plus ribavirin(70%) among patients in the immediate treatment arm. The first 12 wk of the study were blinded. Safety was assessed in d UAL-treated patients compared with placebo patients during the first 12 wk(doubleblind phase), and during 24 wk of d UAL in both arms combined.RESULTS In total, 207 patients were randomly assigned to immediate(n = 155) or placebo-deferred(n = 52) treatment. Most patients were Asian(86%), female(59%) and aged < 65 years(90%). Among them, 13% had cirrhosis, 32% had IL28 B non-CC genotypes and 53% had baseline HCV RNA levels of ≥ 6 million IU/m L. Among patients in the immediate treatment arm, SVR12 was achieved by 92%(95% confidence interval: 87.2-96.0), which was significantly higher than the historical comparator rate(70%). SVR12 was largely unaffected by cirrhosis(89%), age ≥ 65 years(92%), male sex(90%), baseline HCV RNA ≥ 6 million(89%) or IL28 B non-CC genotypes(96%), although SVR12 was higher among patients without(96%) than among those with(53%) baseline NS5 A resistanceassociated polymorphisms(at L31 or Y93 H). during the double-blind phase, aminotransferase elevations were more common among placebo recipients than among patients receiving d UAL. during 24 wk of d UAL therapy(combined arms), the most common adverse events(≥ 10%) were elevated alanine aminotransferase and upper respiratory tract infection; emergent grade 3-4 laboratory abnormalities were infrequently observed, and all grade 3-4 aminotransferase abnormalities(alanine aminotransferase, n = 9; aspartate transaminase, n = 6) reversed within 8-11 d. Two patients discontinued d UAL treatment; one due to aminotransferase elevations, nausea, and jaundice and the other due to a fatal adverse event unrelated to treatment. There were no treatment-related deaths.CONCLUSION d UAL was well-tolerated during this phase 3 study, and SVR12 with d UAL treatment(92%) exceeded thehistorical SVR rate for peg-interferon plus ribavirin of 70%. | Lai Wei Fu-Sheng Wang Ming-Xiang Zhang Ji-Dong Jia Alexey A Yakovlev Wen Xie Eduard Burnevich Jun-Qi Niu Yong Jin Jung Xiang-Jun Jiang Min Xu Xin-Yue Chen Qing Xie Jun Li Jin-Lin Hou Hong Tang Xiao-guang Dou Yash Gandhi Wen-Hua Hu Fiona McPhee Stephanie Noviello Michelle Treitel Ling Mo Jun Deng | 2018 | World Journal of Gastroenterology2018，24，12： | 17 |
| 8 | Dihydromyricetin alleviates carbon tetrachloride-induced acute liver injury via JNK-dependent mechanism in mice显示文摘AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mice were used in this study. Mice were orally administered with DHM(150 mg/kg) for 4 d after CCl4 treatment. Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment. The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin, and superoxide dismutase(SOD). Inflammatory cytokines, such as interleukin(IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α), were detected using ELISA kits. Proliferating cell nuclear antigen(PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation. Hepatocyte apoptosis wasmeasured by TUNEL assay.Furthermore,apoptosis proteins Caspases-3,6,8,and 9 were detected by Western blot.SP600125 were used to confirm whether DHM regulated liver regeneration through JNK/TNF-αpathways.RESULTS:DHM showed a strong anti-inflammatory effect on CCl4-induced liver injury in mice.DHM could significantly decrease serum ALT,AST,IL-1β,IL-6 and TNF-αand increase serum albumin,SOD and liver SOD compared to the control group after CCl4 treatment(P<0.05).PCNA results indicated that DHM could significantly increase the number of PCNA positive cells compared to the control(348.9±56.0 vs 107.1±31.4,P<0.01).TUNEL assay showed that DHM dramatically reduced the number of apoptotic cells after CCl4 treatment compared to the control(365.4±99.4 vs 90.5±13.8,P<0.01).Caspase activity detection showed that DHM could reduce the activities of Caspases-8,3,6 and 9 compared to the control(P<0.05).The results of Western blot showed that DHM increased the expression of JNK and decreased TNF-αexpression.However,DHM could not affect TNF-αexpression after SP600125 treatment.Furthermore,DHM could significantly improve the survival rate of acute liver failure(ALF)mice(73.3%vs 20.0%,P<0.0001),and SP600125 could inhibit the effect of DHM.CONCLUSION:These findings demonstrate that DHM alleviates CCl4-induced liver injury,suggesting that DHM is a promising candidate for reversing liver injury and ALF. | Jun Xie Jie Liu Tu-Ming Chen Qing Lan Qing-Yu Zhang Bin Liu Dong Dai Wei-Dong Zhang Li-Ping Hu Run-Zhi Zhu | 2015 | World Journal of Gastroenterology2015，21，18： | 17 |
| 9 | Detection,isolation,and identification of cadmium-resistant bacteria based on PCR-DGGE显示文摘这研究集中于从跟踪的 Pb-Zn 屏蔽镉抵抗的菌株。我们调查了微生物引起的社区住在 Dong-san-cha Pb-Zn 在中国北京跟踪的差异,由聚合酶链菌株的 16S rRNA 基因的使中毒反应的坡度胶化电气泳动(DGGE ) ,并且在 DGGE 侧面发现二主导的紧张。用特殊文化媒介,我们孤立二强壮的镉抵抗的菌株。根据定序的词法、生理、生物化学的特征, BIOLOG,和 16S rDNA,二紧张作为杆菌仙影拳和 Enterobacter 下水道被识别。为细菌的重金属的最小的禁止的集中(MIC ) 是坚定的。E。下水道比 B 为重金属和抗菌素抵抗的一个更大的范围显示出更高的 MIC 价值。仙影拳。 | HU Qing DOU Min-na QI Hong-yan XIE Xiang-ming ZHUANG Guo-qiang YANG Min | 2007 | Journal of Environmental Sciences2007，19，9： | 16 |
| 10 | Edaravone (MC1-186), a free radical scavenger, attenuates retinal ischemia/reperfusion injury in rats显示文摘瞄准:调查 edaravone (MCI-186 ) 的效果,自由基 scavenger,对 ischemia/reperfusion (I/R ) 在老鼠视网膜的损害。方法:网膜的局部缺血被为 60 min 提高 intraocular 压力到 110 毫米汞柱在男 Sprague-Dawley 老鼠导致。老鼠 intraperitoneally 在局部缺血前在 30 min 在 3 mg/kg 的剂量与 edaravone 被注射,然后与 edaravone 对待(3 mg/kg, ip ) 为在 I/R 以后的 1 或 5 d 两次每日。malondialdehyde (MDA ) 和在网膜的纸巾的过氧化物歧化酶(草皮) 的层次在 d 上被决定 1 在 I/R 损害以后。网膜的神经原的 apoptosis 在 d 上被检测 1 在由终端 deoxynucleotidyl 的 I/R 损害以后标记染色的调停 transferase 的 digoxigenin-dUTP 刻痕结束。网膜电图(尔格) 在 d 上被记录 5 在灌注以后。结果:Edaravone 降低了 MDA 层次,提起的草皮活动,和在内的网膜的神经原的稀释 I/R-induced apoptosis 内部原子,老鼠视网膜的中心房间,和外胞核层。而且, edaravone 在一波浪和尔格的 b 波浪振幅压制了 I/R-induced 减小。结论:Edaravone 能通过减少氧化应力在老鼠保护视网膜免于 I/R 损害,禁止网膜的神经原的 apoptosis,它建议那 edaravone 可能是为 I/R-induced 眼睛混乱的处理的一种潜在的选择。 | Yi SONG Yuan-yuan GONG Zheng-gao XIE Cai-hong LI Qing GU Xing-wei WU | 2008 | Acta Pharmacologica Sinica2008，29，7： | 15 |
| 11 | A Cross-sectional Survey Assessing Carriage of Streptococcus pneumoniae in a Healthy Population in Xinjiang Uygur Autonomous Region of China显示文摘链球菌 pneumoniae 的车厢率和 serotype 分发(S。pneumoniae ) 在在中国遗体的一张健康人口不清楚。在这研究,我们在 Xinjiang 从 513 个个人收集了 oropharyngeal 拖把,中国。指向 lytA 基因和 12 serotypes 的实时 PCR 被估计识别 S。pneumoniae 马车。S 的全部的车厢率。pneumoniae 是 70.4%(361/513 ) 。最流行的 serotypes 是 19B/F, 18B/C, 5,并且 6A/B。S 的最高的车厢率。pneumoniae 6-10 年(88.6%) 在孩子被注意,它应得进一步的注意。二的合作殖民率或更多的 S。pneumoniae serotypes 是 79.8%(264/331 ) 。这研究试图在中国在健康个人之中调查基线 pneumococcal 马车率改进我们 S 的传染病学的理解。pneumoniae。 | XIE Na CHEN Zhao Yun CHEN Tao ZHU Bing Qing XU Li GAO Yuan ZHANG Ai Yu ZHAO Pan LIU Ji Wen SHAO Zhu Jun | 2018 | Biomedical and Environmental Sciences2018，31，3： | 15 |
| 12 | Principles and technology for stepwise utilization of resources for mitigating deep mine heat hazards显示文摘As is well known, deep mines are hot. As mining depth increases, the temperature of the surrounding rock also increases. This seriously affects mine safety and production and has restricted the exploitation of deep coal resources. Therefore, reducing the working face temperature to improve working conditions by controlling these heat hazards is an urgent problem. Considering problems in cooling deep mines both domestically and abroad along with the actual conditions of the Zhangshuanglou coal mine, we propose a HEMS technology that uses heat resources from deep mines in a stepwise manner. HEMS means a high temperature ex-change machinery system. Mine inrush-water is used as a source of cooling. Twice the energy is extracted from the mine inrush water. Heat is used for building heating in the winter and cold water is used for cooling buildings in the summer. This opens a new technology for stepwise utilization of heat energy in deep mines. Energy conservation and reduced pollution, an improved environment and sustainable economic development are realized by this technique. The economic and social effects are obvious and illustrate a good prospect for the application and extension of the method. | HE Manchao CAO Xiuling XIE Qiao YANG Jiahua QI Ping YANG Qing CHEN Xueqian | 2010 | Mining Science and Technology2010，20，1： | 15 |
| 13 | Bacterial infection triggers and complicates acute-on-chronic liver failure in patients with hepatitis B virus-decompensated cirrhosis: A retrospective cohort study显示文摘BACKGROUND Reports on bacterial infection(BI)in decompensated cirrhosis(DC)is mainly from alcoholic cirrhosis.The role of BI as a trigger or complication of acute-onchronic liver failure(ACLF)in patients with hepatitis B virus decompensated cirrhosis(HBV-DC)remains to be investigated.AIM To investigate the impact of BI on the outcomes of the patients with HBV-DC admitted into the hospital with or without ACLF.METHODS This retrospective study included patients with HBV-DC admitted to two tertiary centers in China.In-hospital overall survival,90-d transplant-free survival,5-year post-discharge survival,and cumulative incidence of ACLF were evaluated.Risk factors for death were analyzed considering liver transplantation as a competing event.RESULTS A total of 1281 hospitalized HBV-DC patients were included;284 had ACLF at admission.The overall prevalence of BI was 28.1%.The patients with BI had a significantly lower in-hospital survival and transplant-free 90-d survival than those without,in both the patients admitted with and without ACLF.The presence of BI significantly increased the risk of developing ACLF[subdistribution hazard ratio(sHR)=2.52,95%CI:1.75-3.61,P<0.001]in the patients without ACLF.In the patients discharged alive,those who had an episode of BI had a significantly lower 5-year transplant-free survival.BI was an independent risk factor for death in the patients admitted without ACLF(sHR=3.28,95%CI:1.93-5.57),while in ACLF admissions,the presence of pneumonia,but not other type of BI,independently increased the risk of death(sHR=1.87,95%CI:1.24-2.82).CONCLUSION BI triggers ACLF in patients with HBV-DC and significantly impairs short-term survival.HBV-DC patients should be monitored carefully for the development of BI,especially pneumonia,to avoid an adverse outcome. | Zhu-Jun Cao Yu-Han Liu Chuan-Wu Zhu Shan Yin Wei-Jing Wang Wei-Liang Tang Gang-De Zhao Yu-Min Xu Lu Chen Tian-Hui Zhou Ming-Hao Cai Hui Wang Wei Cai Shi-San Bao Hai Li Qing Xie | 2020 | World Journal of Gastroenterology2020，26，6： | 15 |
| 14 | Baicalein ameliorates ulcerative colitis by improving intestinal epithelial barrier via AhR/IL-22 pathway in ILC3s显示文摘Ulcerative colitis(UC)is a chronic inflammatory disease of the gastrointestinal tract,which is closely related to gut barrier dysfunction.Emerging evidence shows that interleukin-22(IL-22)derived from group 3 innate lymphoid cells(ILC3s)confers benefits on intestinal barrier,and IL-22 expression is controlled by aryl hydrocarbon receptor(AhR).Previous studies show that baicalein protects the colon from inflammatory damage.In this study we elucidated the molecular mechanisms underlying the protective effect of baicalein on intestinal barrier function in colitis mice.Mice were administered baicalein(10,20,40 mg·kg^(-1)·d^(-1),i.g.)for 10 days;the mice freely drank 3%dextran sulfate sodium(DSS)on D1-D7 to induce colitis.We showed that baicalein administration simultaneously ameliorated gut inflammation,decreased intestinal permeability,restored tight junctions of colons possibly via promoting AhR/IL-22 pathway.Co-administration of AhR antagonist CH223191(10 mg/kg,i.p.)partially blocked the therapeutic effects of baicalein in colitis mice,whereas AhR agonist FICZ(1μg,i.p.)ameliorated symptoms and gut barrier function in colitis mice.In a murine lymphocyte line MNK-3,baicalein(5-20μM)dose-dependently increased the expression of AhR downstream target protein CYP1A1,and enhanced IL-22 production through facilitating AhR nuclear translocation,these effects were greatly diminished in shAhR-MNK3 cells,suggesting that baicalein induced IL-22 production in AhR-dependent manner.To further clarify that,we constructed an in vitro system consisting of MNK-3 and Caco-2 cells,in which MNK-3 cell supernatant treated with baicalein could decrease FITC-dextran permeability and promoted the expression of tight junction proteins ZO-1 and occluding in Caco-2 cells.In conclusion,this study demonstrates that baicalein ameliorates colitis by improving intestinal epithelial barrier via AhR/IL-22 pathway in ILC3s,thus providing a potential therapy for UC. | Yan-yang Li Xiao-jing Wang Yu-lin Su Qing Wang Shao-wei Huang Zeng-feng Pan Yan-ping Chen Jun-jie Liang Mei-ling Zhang Xue-qian Xie Zhi-yun Wu Jin-yan Chen Lian Zhou Xia Luo | 2022 | Acta Pharmacologica Sinica2022，43，6： | 14 |
| 15 | Effect of human umbilical cord-derived mesenchymal stem cells on lung damage in severe COVID-19 patients:a randomized,double-blind,placebo-controlled phase 2 trial显示文摘Treatment of severe Coronavirus Disease 2019(COVID-19)is challenging.We performed a phase 2 trial to assess the efficacy andsafety of human umbilical cord-mesenchymal stem cells(UC-MScs)to treat severe coViD-19 patients with lung damage,based onour phase 1 data.In this randomized,double-blind,and placebo-controlled trial,we recruited 101 severe coVID-19 patients withlung damage.They were randomly assigned at a 2:1 ratio to receive either UC-MSCs(4×10^(7)cells per infusion)or placebo on day 0,3,and 6.The primary endpoint was an altered proportion of whole lung lesion volumes from baseline to day 28.Other imagingoutcomes,6-minute walk test(6-MWT),maximum vital capacity,diffusing capacity,and adverse events were recorded and analyzed.In all,100 COVID-19 patients were finally received either UC-MSCs in=65)or placebo(n=35).UC-MSCs administrationexerted numerical improvement in whole lung lesion volume from baseline to day 28 compared with the placebo(the mediandifference was-13.31%,95%Cl-29.14%,2.13%,P=0.08).UC-MSCs significanty reduced the proportions of solid componentlesion volume compared with the placebo(median difference:-15.45%;95%CI-30.82%,-0.39%;P=0.043).The 6-MWT showedan increased distance in patients treated with UC-MSCs(difference:27.00 m;95%CI 0.00,57.00;P=0.057).The incidence of adverseevents was similar in the two groups.These results suggest that UC-MSCs treatment is a safe and potentially effective therapeuticapproach for COVID-19 patients with lung damage.A phase 3 trial is required to evaluate effects on reducing mortality andpreventing long-term pulmonary disability. | Lei Shi Hai Huang Xuechun Lu Xiaoyan Yan Xiaojing Jiang Ruonan Xu Siyu Wang Chao Zhang Xin Yuan Zhe Xu Lei Huang Jun-Liang Fu Yuanyuan Li Yu Zhang Wei-Qi Yao Tianyi Liu Jinwen Song Liangliang Sun Fan Yang Xin Zhang Bo Zhang Ming Shi Fanping Meng Yanning Song Yongpei Yu Jiqiu Wen Qi Li Qing Mao Markus Maeurer Alimuddin Zumla Chen Yao Wei-Fen Xie Fu-Sheng Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 13 |
| 16 | Isolation, identification and genomic characterization of the Asian lineage Zika virus imported to China显示文摘Dear Editor,Zika virus(ZIKV)used to be an unknown mosquito-borne flavivirus,and maintained its limited sylvatic circulation in a few African and Asian countries(Enfissi et al.,2016).Based on available clinical data,the symptoms in human infections with ZIKV are supposed to be similar to other arbovirus infections such as dengue,and characterized by fever,skin rashes,conjunctivitis,muscle and joint pain,malaise,and headache(Duffy et al.,2009).However。 | Yong-Qiang Deng Hui Zhao Xiao-Feng Li Na-Na Zhang Zhong-Yu Liu Tao Jiang Da-Yong Gu Lei Shi Jian-An He Hong-Jiang Wang Zhao-Zeng Sun Qing Ye Dong-Yang Xie Wu-Chun Cao Cheng-Feng Qin | 2016 | Science China(Life Sciences)2016，59，4： | 13 |
| 17 | Combined effect of repetitive transcranial magnetic stimulation and physical exercise on cortical plasticity显示文摘Physical exercise can minimize dysfunction and optimize functional motor recovery after stroke by modulating cortical plasticity.However,the limitation of physical exercise is that large amounts of time and effort are necessary to significantly improve motor function,and even then,substantial exercise may not be sufficient to normalize the observed improvements.Thus,interventions that could be used to strengthen physical exercise-induced neuroplasticity may be valuable in treating hemiplegia after stroke.Repetitive transcranial magnetic stimulation seems to be a viable strategy for enhancing such plasticity.As a non-invasive cortical stimulation technique,repetitive transcranial magnetic stimulation is able to induce longterm plastic changes in the motor system.Recently,repetitive transcranial magnetic stimulation was found to optimize the plastic changes caused by motor training,thereby enhancing the long-term effects of physical exercise in stroke patients.Therefore,it is believed that the combination of repetitive transcranial magnetic stimulation and physical exercise may represent a superior method for restoring motor function after stroke. | Ya-Wen Yang Wen-Xiu Pan Qing Xie | 2020 | Neural Regeneration Research2020，15，11： | 13 |
| 18 | Assessment of left ventricular torsion in patients with anterior wall myocardial infarction before and after revascularization using speckle tracking imaging显示文摘左的背景旋转室( LV )顶到底,或 LV 扭转,与心肌的收缩性和结构有关并且最近作为心脏的性能的敏感指示物被认出了,但是估计 LV torsion.This 的一个强大的工具学习的点缀追踪成像( STI )可以提供的 2-dimensional ( 2D )的最近的开发被进行与前面的墙在病人评估全球、地区性的 LV 扭曲,对 measure.The 困难心肌的梗塞( AMI ) | HAN Wei XIE Ming-xing WANG Xin-fang LU Qing WANG Jing ZHANG li ZHANG Jing | 2008 | Chinese Medical Journal2008，，16： | 12 |
| 19 | Trabecular-like Ti-6Al-4V scaffolds for orthopedic: fabrication by selective laser melting and in vitro biocompatibility显示文摘Porous metal scaffolds play an important role in the orthopedic field, due to their wide applications in prostheses implantation. Some previous studies showed that the scaffolds with trabecular bone structure reconstructed via computed tomography had satisfactory biocompatibility. However, the reverse modeling scaffolds were inflexible for customized design. Therefore, a top-down designing biomimetic bone scaffold with favorable mechanical performances and cytocompatibility is urgently demanded for orthopedic implants. An emerging additive manufacturing technique, selective laser melting, was employed to fabricate the trabecular-like porous Ti-6Al-4 V scaffolds with varying irregularities(0.05-0.5) and porosities(48.83%–74.28%) designed through a novel Voronoi-Tessellation based method. Micro-computed tomography and scanning electron microscopy were used to characterize the scaffolds’ morphology.Quasi-static compression tests were performed to evaluate the scaffolds’ mechanical properties. The MG63 cells culture in vitro experiments, including adhesion, proliferation, and differentiation, were conducted to study the cytocompatibility of scaffolds. Compressive tests of scaffolds revealed an apparent elastic modulus range of 1.93–5.24 GPa and an ultimate strength ranging within 44.9–237.5 MPa, which were influenced by irregularity and porosity, and improved by heat treatment. Furthermore, the in vitro assay suggested that the original surface of the SLM-fabricated scaffolds was favorable for osteoblasts adhesion and migration because of micro scale pores and ravines. The trabecular-like porous scaffolds with full irregularity and higher porosity exhibited enhanced cells proliferation and osteoblast differentiation at earlier time, due to their preferable combination of small and large pores with various shapes. This study suggested that selective laser melting-derived Ti-6Al-4 V scaffold with the trabecular-like porous structure designed through Voronoi-Tessellation method, favorable mechanical performance, and good cytocompatibility was a potential biomaterial for orthopedic implants. | Huixin Liang Youwen Yang Deqiao Xie Lan Li Ning Mao Changjiang Wang Zongjun Tian Qing jiang Lida Shen | 2019 | Journal of Materials Science & Technology2019，35，7： | 12 |
| 20 | A serum metabonomic study on the difference between alcoholand HBV-induced liver cirrhosis by ultraperformance liquid chromatography coupled to mass spectrometry plus quadrupole time-of-flight mass spectrometry显示文摘背景肝肝硬化是长期的肝炎的致命的后果,使肝肝硬化的早诊断批评。肝活体检视仍然是为肝肝硬化的标准诊断方法,尽管它在有酒精中毒或感染仍然是的肝炎 B 病毒(HBV ) 的一张宽广人口的使用困难。在这研究,我们使用了一条 metabonomic 途径检测潜在的 biomarkers 因为肝 cirrhosis.Methods 浆液标本的早诊断与含酒精的肝硬化(n=18 ) 或导致 HBV 的肝硬化(n=19 ) 来自正常控制题目(n=22 ) 和病人有希望地是镇定的。浆液 metabonome 用 ultraperformance 液体被分析层析(LC ) Rime-of-flight 集体 spectrometry (MS ) 与 chemometrics 综合。获得的最小公倍数数据被使正常化并且处理了使用主要部件分析(PCA ) 和部分最少的广场辨别分析(PLS-DA ).Results 在在三个组之中的 metabonomics 的重要差别被观察。 Lysophosphatidyl 胆碱( LPC )( LPC C16:0 , LPC C18:0 , LPC C18:2 , LPC C18:3 , LPC C20:3 , LPC C20:5 )与肝的肝硬化在病人的浆液被减少胆汁酸( glycocholic 酸, glycochenodeoxycholic 酸), hypoxanthine ,并且 stearamide 与肝的肝硬化在病人的浆液被增加。这些代谢物为肝的肝硬化被认为 biomarkers “普通”。Oleamide 和 myristamide 与含酒精的肝硬化在病人的浆液被增加,但是与导致 HBV 的肝硬化在那些减少了。这些能是特定的 biomarkers 因为在在那里的导致白酒、导致 HBV 的肝的 cirrhosis.Conclusions 之间的微分诊断是导致白酒、导致 HBV 的肝 cirrhosis.Metabonomics 之间的重要 metabonomic 差别是为在临床的问题上进行研究的一个自顶向下的系统生物学工具。 | LIAN Jiang-shan LIU Wei HAO Shao-rui GUO Yong-zheng HUANG Hai-jun CHEN De-ying XIE Qing PAN Xiao-ping XU Wei YUAN Wen-xia LI Lan-juan HUANG Jian-rong | 2011 | Chinese Medical Journal2011，，9： | 11 |
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