|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A strategy for generating rice apomixis by gene editing显示文摘Apomixis is an asexual reproduction way of plants that can produce clonal offspring through seeds.In this study,we introduced apomixis into rice(Oryza sativa)by mutating OsSPO11-1,OsREC8,OsOSD1,and OsMATL through a CRISPR/Cas9 system.The quadruple mutant showed a transformation from meiosis to mitosis and produced clonal diploid gametes.With mutated Osmatl,which gives rise to haploid induction in plants,the quadruple mutant is expected to be able to be produced apomictic diploid offspring.We named this quadruple mutant as AOP(Apomictic Offspring Producer)for its ability to produce apomictic offspring. | En Xie Yafei Li Ding Tang Yanli Lv Yi Shen Zhukuan Cheng | 2019 | Journal of Integrative Plant Biology2019，61，8： | 6 |
| 2 | Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells显示文摘Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by their low transfection efficiency.Here,we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells.To reach such a purpose,the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli.The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.Afterwards,the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology.Finally,the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells.Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure(approximately 100 nm)and high transfection efficiency into lung cancer and cancer stem cells.The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells.In conclusion,the targeting tLyp-1 exosomes are successfully engineered,and can be used for gene therapy with a high transfection efficiency.Therefore,the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy. | Jing Bai Jialun Duan Rui Liu Yafei Du Qian Luo Yinuo Cui Zhanbo Su Jiarui Xu Ying Xie Wanliang Lu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 5 |
| 3 | A cross-linked fluorinated poly (aryl ether oxadiazole) s using a thermal cross-linking for anion exchange membranes显示文摘 | Cunpu Li Shubo Wang Wenpin Wang Xiaofeng Xie Yafei Lv Changsheng Deng | 2013 | International Journal of Hydrogen Energy2013，，： | 3 |
| 4 | Synthesis and characterization of a fluorinated cross-linked anion exchange membrane显示文摘 | Wenpin Wang Shubo Wang Weiwei Li Xiaofeng Xie Yafei lv | 2013 | International Journal of Hydrogen Energy2013，，： | 2 |
| 5 | 3-Oxodapagliflozin as a Potent and Highly Selective SGLT2 Inhibitor for the Treatment of Type 2 Diabetes显示文摘 | ZHANG Shuo WANG Yuli LIU Wei XIE Yafei LIU Yuqiang XU Weiren TANG Lida WANG Jianwua ZHAO Guilong | 2014 | Chemical Research in Chinese Universities2014，30，5： | 2 |
| 6 | Balancing sub‐reaction activity to boost electrocatalytic urea synthesis using a metal‐free electrocatalyst显示文摘Electrocatalytic urea synthesis via coupling of nitrate with CO_(2)is considered as a promising alternative to the industrial urea synthetic process.However,the requirement of sub-reaction(NO_(3)RR and CO_(2)RR)activities for efficient urea synthesis is not clear and the related reaction mechanisms remain obscure.Here,the construction,breaking,and rebuilding of the sub-reaction activity balance would be accompanied by the corresponding regulation in urea synthesis,and the balance of sub-reaction activities was proven to play a vital role in efficient urea synthesis.With rational design,a urea yield rate of 610.6 mg h−1 gcat.−1 was realized on the N-doped carbon electrocatalyst,superior to that of noble-metal electrocatalysts.Based on the operando SRFTIR measurements,we proposed that urea synthesis arises from the coupling of^(*)NO and^(*)CO to generate the key intermediate of^(*)OCNO.This work provides new insights and guidelines into urea synthesis from the aspect of activity balance. | Chen Chen Shuang Li Xiaorong Zhu Shuowen Bo Kai Cheng Nihan He Mengyi Qiu Chao Xie Dezhong Song Youzhen Liu Wei Chen Yafei Li Qinghua Liu Conggang Li Shuangyin Wang | 2023 | Carbon Energy2023，5，10： | 2 |
| 7 | Design, Synthesis and Biological Activity of Tetrazole-bearing Uric Acid Transporter 1 Inhibitors显示文摘 | CAI Wenqing LIU Wei XIE Yafei WU Jingwei LIU Yuqiang LIU Changying XU Weiren TANG Lida WANG Jianwu ZHAO Guilong | 2017 | Chemical Research in Chinese Universities2017，33，1： | 2 |
| 8 | Flat gain spectrum design of Raman fiber amplifiers based on particle swarm optimization and average power analysis technique显示文摘 | Haiming Jiang Kang Xie Yafei Wang | 2012 | Optics and Lasers in Engineering2012，50，2： | 1 |
| 9 | Combined effect of eypennethrin and copper on activity in soil显示文摘 | Liu Jun Xie Jimin Chu Yafei | 2008 | Journal of Soil Sediments2008，8，5： | 1 |
| 10 | Integrated behavior simulation and verification for a MEMS vibratory gyroscope using parametric model order reduction 显示文摘 | Chang Honglong Zhang Yafei Xie Jianbing | 2010 | Microelectromechanical Systems2010，19，2： | 1 |
| 11 | Homotypic clustering of L1 and B1/Alu repeats compartmentalizes the 3D genome显示文摘Organization of the genome into euchromatin and heterochromatin appears to be evolutionarily conserved and relatively stable during lineage differentiation.In an effort to unravel the basic principle underlying genome folding,here we focus on the genome itself and report a fundamental role for L1(LINE1 or LINE-1)and B1/Alu retrotransposons,the most abundant subclasses of repetitive sequences,in chromatin compartmentalization.We find that homotypic clustering of L1 and B1/Alu demarcates the genome into grossly exclusive domains,and characterizes and predicts Hi-C compartments.Spatial segregation of L1-rich sequences in the nuclear and nucleolar peripheries and B1/Alu-rich sequences in the nuclear interior is conserved in mouse and human cells and occurs dynamically during the cell cycle.In addition,de novo establishment of L1 and B1 nuclear segregation is coincident with the formation of higher-order chromatin structures during early embryogenesis and appears to be critically regulated by L1 and B1 transcripts.Importantly,depletion of L1 transcripts in embryonic stem cells drastically weakens homotypic repeat contacts and compartmental strength,and disrupts the nuclear segregation of L1-or B1-rich chromosomal sequences at genome-wide and individual sites.Mechanistically,nuclear co-localization and liquid droplet formation of L1 repeat DNA and RNA with heterochromatin protein HP1αsuggest a phase-separation mechanism by which L1 promotes heterochromatin compartmentalization.Taken together,we propose a genetically encoded model in which L1 and B1/Alu repeats blueprint chromatin macrostructure.Our model explains the robustness of genome folding into a common conserved core,on which dynamic gene regulation is overlaid across cells. | J.Yuyang Lu Lei Chang Tong Li Ting Wang Yafei Yin Ge Zhan Xue Han Ke Zhang Yibing Tao Michelle Percharde Liang Wang Qi Peng Pixi Yan Hui Zhang Xianju Bi Wen Shao Yantao Hong Zhongyang Wu Runze Ma Peizhe Wang Wenzhi Li Jing Zhang Zai Chang Yingping Hou Bing Zhu Miguel Ramalho-Santos Pilong Li Wei Xie Jie Na Yujie Sun Xiaohua Shen | 2021 | Cell Research2021，31，6： | 1 |
| 12 | Combined effect of cypermethrin and copper on catalase activity in soil显示文摘 | Jun Liu Jimin Xie Yafei Chu Cheng Sun Chuanxiang Chen Qian Wang | 2008 | Journal of Soils and Sediments2008，，5： | 1 |
| 13 | Density Functional Theory Study of Hydroxide-ion Induced Degradation of Imidazolium Cations显示文摘 | Wenpin Wang Shubo Wang Xiaofeng Xie Yafei lv Vijay Ramani | 2014 | International Journal of Hydrogen Energy2014，，： | 1 |
| 14 | Efficient extraction of input pump powers from pump power integrals in the optimization of Raman fiber amplifiers显示文摘 | Haiming Jiang Kang Xie Yafei Wang | 2010 | J Lightwave Technol2010，28，13： | 1 |
| 15 | Physical Activity,Sedentary Behavior,and the Risk of Cardiovascular Disease in Type 2 Diabetes Mellitus Patients:The MIDiab Study显示文摘The aim of this study was to explore the associations of moderate-to-vigorous-intensity physical activity(MVPA)time and sedentary(SED)time with a history of cardiovascular disease(CVD)and multifactorial(i.e.,blood pressure(BP),body mass index(BMI),low-density lipoprotein cholesterol(LDL-C),and glycated hemoglobin A1c(HbA1c))control status among type 2 diabetes mellitus(T2DM)patients in China.A cross-sectional analysis of 9152 people with type 2 diabetes from the Multifactorial Intervention on Type 2 Diabetes(MIDiab)study was performed.Patients were grouped according to their self-reported MVPA time(low,<150 min·week−1;moderate,150 to<450 min·week−1;high,≥450 min·week−1)and SED time(low,<4 h·d–1;moderate,4 to<8 h·d–1;high,≥8 h·d–1).Participants who self-reported a history of CVD were identified as having a CVD risk.Odds ratios(ORs)and 95%confidence intervals(CIs)of CVD risk and multifactorial control status associated with MVPA time and SED time were estimated using mixed-effect logistic regression models,adjusting for China’s geographical region characteristics.The participants had a mean±standard deviation(SD)age of(60.87±8.44)years,44.5%were women,and 25.1%had CVD.After adjustment for potential confounding factors,an inverse association between high MVPA time and CVD risk that was independent of SED time was found,whereas this association was not observed in the moderate-MVPA group.A higher MVPA time was more likely to have a positive effect on the control of BMI.Compared with the reference group(i.e.,those with MVPA time≥450 min·week−1 and SED time<4 h·d–1),CVD risk was higher in the low-MVPA group:The OR associated with an SED time<4 h·d–1 was 1.270(95%CI,1.040–1.553)and that associated with an SED time≥8 h·d–1 was 1.499(95%CI,1.149–1.955).We found that a high MVPA time(i.e.,≥450 min·week−1)was associated with lower odds of CVD risk regardless of SED time among patients with T2DM. | Yafei Wu Guijun Qin Guixia Wang Libin Liu Bing Chen Qingbo Guan Zhongshang Yuan Xu Hou Ling Gao Chao Xu Haiqing Zhang Xu Zhang Qiu Li Yongfeng Song Fei Jing Shizhan Ma Shanshan Shao Meng Zhao Qingling Guo Nanwei Tong Hongyan Zhao Xiaomin Xie Chao Liu Zhongyan Shan Zhifeng Cheng Xuefeng Yu Shulin Chen Tao Yang Yangang Wang Dongmei Li Zhaoli Yan Lixin Guo Qiuhe Ji Wenjuan Wang Jiajun Zhao | 2023 | Engineering2023，，1： | 1 |
| 16 | Pump scheme for gain-flattened Raman fiber amplifiers using improved particle swarm optimization and modified shooting algorithm显示文摘 | Haiming Jiang Kang Xie Yafei Wang | 2010 | Opt Express2010，18，11： | 1 |
| 17 | Shooting algorithm and particle swarm optimization based Raman fiber amplifiers gain spectra design显示文摘 | Haiming Jiang Kang Xie Yafei Wang | 2010 | Oot Commun2010，283，13： | 1 |
| 18 | Optimization of pump parameters for gain flattened Raman fiber amplifiers based on artificial fish school algorithm显示文摘 | Haiming Jiang Kang Xie Yafei Wang | 2011 | Opt Commun2011，284，23： | 1 |
| 19 | Novel design of flat gain spectrum Raman fiber amplifiers based on ant colony optimization显示文摘 | Haiming Jiang Kang Xie Yafei Wang | 2011 | IEEE Phonton Technol Lett2011，23，23： | 1 |
| 20 | A model for thermal annealing on forming In—N clusters in InGaNP显示文摘We develop a model for the effect of thermal annealing on forming In-N clusters in GaInNP according to thermodynamics.The average energy variation for forming an In-N bond in the model is estimated according to the theoretical calculation.Using the model,the added number of In-N bonds per mol of InGaNP,the added number of nearest-neighbor In atoms per N atom and the average number of nearest-neighbor In atoms per N atom after annealing are calculated.The different function of In-N clusters in InGaNP and InGaN is also discussed,which is due to the different environments around the In-N clusters. | ZHAO ChuanZhen CHEN Lei LI NaNa ZHANG HuanHuan CHEN YaFei WEI Tong TANG ChunXiao XIE ZiLi | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员