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| 1 | Ovarian cancer classification based on dimensionality reduction for SELDI-TOF data显示文摘 | TANG Kailin LI T0nghua XIONG Wenwei | 2010 | BMC Bioinformatics2010，11，： | 1 |
| 2 | AI-aided on-chip nucleic acid assay for smart diagnosis of infectious disease显示文摘Global pandemics such as COVID-19 have resulted in significant global social and economic disruption.Although polymerase chain reaction(PCR)is recommended as the standard test for identifying the SARS-CoV-2,conventional assays are time-consuming.In parallel,although artificial intelligence(AI)has been employed to contain the disease,the implementation of AI in PCR analytics,which may enhance the cognition of diagnostics,is quite rare.The information that the amplification curve reveals can reflect the dynamics of reactions.Here,we present a novel AI-aided on-chip approach by integrating deep learning with microfluidic paper-based analytical devices(μPADs)to detect synthetic RNA templates of the SARS-CoV-2 ORF1ab gene.TheμPADs feature a multilayer structure by which the devices are compatible with conventional PCR instruments.During analysis,real-time PCR data were synchronously fed to three unsupervised learning models with deep neural networks,including RNN,LSTM,and GRU.Of these,the GRU is found to be most effective and accurate.Based on the experimentally obtained datasets,qualitative forecasting can be made as early as 13 cycles,which significantly enhances the efficiency of the PCR tests by 67.5%(∼40 min).Also,an accurate prediction of the end-point value of PCR curves can be obtained by GRU around 20 cycles.To further improve PCR testing efficiency,we also propose AI-aided dynamic evaluation criteria for determining critical cycle numbers,which enables real-time quantitative analysis of PCR tests.The presented approach is the first to integrate AI for on-chip PCR data analysis.It is capable of forecasting the final output and the trend of qPCR in addition to the conventional end-point Cq calculation.It is also capable of fully exploring the dynamics and intrinsic features of each reaction.This work leverages methodologies from diverse disciplines to provide perspectives and insights beyond the scope of a single scientific field.It is universally applicable and can be extended to multiple areas of fundamental research. | Hao Sun Linghu Xiong Yi Huang Xinkai Chen Yongjian Yu Shaozhen Ye Hui Dong Yuan Jia Wenwei Zhang | 2022 | Fundamental Research2022，2，3： | 0 |
| 3 | Cloning and Analysis of Chitinase Gene in Bacillus subtilis HAS显示文摘[Objective] The paper was to study the possible mechanism of chitinase gene. [Method] The chitinase gene with inhibitory effect against a variety of pathogenic fungi was cloned and analyzed in this study. [Result] The genome of HAS strain contained an ORF of 834 bp. The homology of coding protein corresponding to ORF with chitosanase [Bacillus subtilis subsp. subtilis str. 168](ref|NP_390566.1) was the highest of 98%(272/277).[Conclusion]Through the analysis of amino acid sequence and secondary structure of the protease, tertiary structure prediction and analysis, HAS strain may play a pivotal role in controlling Sporisorium scitaminea Syd. | Xiong Guoru Zhao Gengfeng Cai Wenwei Wang Jungang Yang Benpeng Zhang Shuzhen | 2019 | Plant Diseases and Pests2019，10，2： | 0 |
| 4 | Expert consensus on prevention and cardiopulmonary resuscitation for cardiac arrest in COVID-19显示文摘Background:Cardiopulmonary resuscitation(CPR)strategies in COVID-19 patients differ from those in patients suffering from cardiogenic cardiac arrest.During CPR,both healthcare and non-healthcare workers who provide resuscitation are at risk of infection.The Working Group for Expert Consensus on Prevention and Cardiopulmonary Resuscitation for Cardiac Arrest in COVID-19 has developed this Chinese Expert Consensus to guide clinical practice of CPR in COVID-19 patients.Main recommendations:1)A medical team should be assigned to evaluate severe and critical COVID-19 for early monitoring of cardiac-arrest warning signs.2)Psychological counseling and treatment are highly recommended,since sympathetic and vagal abnormalities induced by psychological stress from the COVID-19 pandemic can induce cardiac arrest.3)Healthcare workers should wear personal protective equipment(PPE).4)Mouth-to-mouth ventilation should be avoided on patients suspected of having or diagnosed with COVID-19.5)Hands-only chest compression and mechanical chest compression are recommended.6)Tracheal-intubation procedures should be optimized and tracheal-intubation strategies should be implemented early.7)CPR should be provided for 20-30 min.8)Various factors should be taken into consideration such as the interests of patients and family members,ethics,transmission risks,and laws and regulations governing infectious disease control.Changes in management:The following changes or modifications to CPR strategy in COVID-19 patients are proposed:1)Healthcare workers should wear PPE.2)Hands-only chest compression and mechanical chest compression can be implemented to reduce or avoid the spread of viruses by aerosols.3)Both the benefits to patients and the risk of infection should be considered.4)Hhealthcare workers should be fully aware of and trained in CPR strategies and procedures specifically for patients with COVID-19. | Wei Song Jie Wei Xiangdong Jian Deren Wang Yanhong Ouyang Yuanshui Liu Xianjin Du Ying Chen Yingqi Zhang Heping Xu Shuming Xianyu Qiong Ning Xiang Li Xiaotong Han Feng Zhan Tao Yu Wenteng Chen Jun Zhang Wenwei Cai Sheng’ang Zhou Shengyang Yi Yu Cao Xiaobei Chen Shunjiang Xu Zong’an Liang Duohu Wu Fen Ai Zhong Wang Qingyi Meng Yuhong Mi Sisen Zhang Rongjia Yang Shouchun Yan Wenbin Han Yong Lin Chuanyun Qian Wenwu Zhang Yan Xiong Jun Lv Baochi Liu Yan Cao Xiaojun He Xuelian Sun Yufang Cao Tian’en Zhou | 2021 | Asian Pacific Journal of Tropical Medicine2021，14，6： | 0 |
| 5 | Expression of Invertase Genes in Virus-free Sugarcane显示文摘Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings,and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation.In this study,the differences in the expression levels of 3 invertase genes,CWI,SAI and NI,between virusfree and ordinary sugarcane seedlings were analyzed.Compared with ordinary sugarcane plants,the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage,and especially,greatly up-regulated in immature internodes at maturation stage of virus-free plants.The expression of SAI and NI were mainly up-regulated in leaves and immature internodes of virus-free plants at maturation stage,which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants.It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period,and might facilitate the increase of plant biomass. | Jungang WANG Tingting ZHAO Benpeng YANG Wenwei CAI Cuilian FENG Jun ZENG Guoru XIONG Shuzhen ZHANG | 2017 | Agricultural Biotechnology2017，6，4： | 0 |
| 6 | Pathway for high-energy density LiMnFePO_(4) cathodes显示文摘Polyanion cathodes are credited for its thermal stability and better safety,no matter in lithium ion batteries or sodium ion batteries.Polyanion oxides with phosphate groups came to the public's attention in 1997,and the representative material is LiFePO_(4),which has been widely applied and plays a huge role in the field of powder batteries and energy storage system.However,owing to the low lithiation potentials and storage sites,the energy densities of polyanion cathodes have been restricted,resulting of low-endurance and limited application sce-narios.Accordingly,here,we use cheap and environmental friendly raw materials as precursors to synthesis high energy density LiMn_(0.6)Fe_(0.4)PO_(4)@C cathode by a simple spray-drying and high temperature calcination process.The self-designed liquid polyacrylonitrile(LPAN)is added for the intention of nanoparticle coating,conductive network construction and particle granulation.The low-cost and carbon-coated LiMn_(0.6)Fe_(0.4)PO_(4) cathode exhibits excellent reversible capacity,low electrochemical polarization and excellent rate capacity,which maintains 93.5%capacity retention after cycling 1000 times at 5C.The work introduces a new avenue to fabricate olivine structure cathodes with outstanding electrochemical performance for the high energy density lithium ion batteries. | Shaoluan Huang Wenzhong Lin Liewu Li Pei Liu Tao Huang Zhencheng Huang Jinli kong Wei Xiong Wenwei Yu Shenghua Ye Jiangtao Hu Qianling Zhang Jianhong Liu | 2023 | Progress in Natural Science:Materials International2023，33，1： | 0 |
| 7 | Cloning and Analysis of TasA Gene in Bacillus subtilis HAS显示文摘In order to clarify the mechanism for the inhibitory action of the Bacillus subtilis strain HAS on Sporisorium scitamineum(Syd.),which can cause sugarcane smut,the full-length TasA gene which encodes a protein with broad-spectrum antimicrobial activity,was PCR-amplified from HAS,and cloned into pMD18-T vector.Sequence analysis indicated that the full-length Tas A cloned from HAS consisted of 786 nucleotides,and shared 99% homology in nucleotide sequence with the TasA gene sequence published in Genbank(AJ871386.1).It was predicted that the molecular weight of Tas A protein was about 28 kD.Base transitions or transversions occurred at positions 104,164,169,250,399,623 and 627,at the 2^(nd),2^(nd),3^(rd),1^(st),3^(rd),2^(nd) and 3^(rd) bases of TasA codons.The mutations in the seven bases may cause the missense mutations of the polypeptide chain.Compared with the amino acid sequences of the TasA protein encoded by Bacillus subtilis subsp.subtilis str.168,mutations in two amino acids at positions 150 and 209of the protein encoded by the cloned TasA gene were found,and as a result,an alanine was replaced with a threonine,and a glutamic acid was replaced with an asparagine. | Guoruo XIONG Gengfeng ZHAO Wenwei CAI Suran WU Benpeng YANG Shuzhen ZHANG | 2017 | Agricultural Biotechnology2017，6，2： | 0 |
| 8 | Resistance Evaluation of 23 Sugarcane Varieties to Smut显示文摘[Objective] The paper aimed to screen sugarcane varieties and species layout suitable for Hainan sugarcane area.[Method] Using mixed spores of Ustilago scitaminea Sydow,smut resistance of 21 major sugarcane varieties in national sugarcane regional trial as well as ROC22 and ROC16 were identified through artificial inoculation test and field natural incidence.[Result] Yunzhe 99-596,Yuegan 42,Guitang 29 and Funong 39 reached highly resistant and resistant levels to smut in artificial inoculation test.[Conclusion] Most of the varieties showed better resistance to smut in newly planted sugarcane and ratoon sugarcane except Yunzhe 03-103,Liucheng 03-1137 and ROC22. | Xiong Guoru Cai Wenwei Wang Wenzhi Wu Suran Yang Benpeng Zhang Shuzhen | 2017 | Plant Diseases and Pests2017，8，3： | 0 |
| 9 | Screening and Identification of Antagonistic Strain HAS against Sporisorium scitaminea Sydow显示文摘The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showed that 16 S rDNA gene fragment with the size of 1 533 bp was obtained,and its sequence had the homology of 99% with the sequence of Bacillus subtilis. The morphological characteristics of the strain were as follows: the mycelium of strain HAS was short-rod shape,and could move; Gram staining was positive; 3% KOH solubility test was negative; flagellum was grown around the cells; spores were grown in the middle,elliptic,and cysts were slightly swelled. The strain was preliminarily identified as B. subtilis. Dual culture with pathogenic fungi showed that the strain had good inhibitory effect against various pathogenic fungi causing diseases on sugarcane and other crops. Therefore,strain HAS isolated in the study was identified to be B.subtilis,which had good control effect against S. scitaminea. | Xiong Guoru Zhao Gengfeng Wu Suran Feng Cuilian Cai Wenwei Zhang Shuzhen | 2014 | Plant Diseases and Pests2014，5，3： | 0 |
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