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| 1 | Dynamic Expression of mi RNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings显示文摘Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. Micro RNAs(miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber(Cucumis sativus) scions onto pumpkin(Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated graftinginduced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target m RNAs were detected by quantitative real-time PCR(q RT-PCR) in leaves and roots of hetero-grafted cucumber seedlings(cucumber as scion and pumpkin as rootstock) and autografted cucumber seedlings(cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3 h of drought stress, and then induced after 8 h and 24 h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1 h, but induced after 3 h and 8 h, then reduced significant y after24 h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance. | LI Chaohan LI Yansu BAI Longqiang HE Chaoxing YU Xianchang | 2016 | Horticultural Plant Journal2016，2，1： | 5 |
| 2 | Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings显示文摘The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic stress.However,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber seedlings.Our results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT plants.Physiological studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme activities.qRT-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT plants.Our results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants. | Yumei Liu Qinghua Du Longqiang Bai Mintao Sun Yansu Li Chaoxing He Jun Wang Xianchang Yu Yan Yan | 2021 | Horticultural Plant Journal2021，7，3： | 3 |
| 3 | Sugars promote graft union development in the heterograft of cucumber onto pumpkin显示文摘The use of heterografts is widely applied for the production of several important commercial crops,but the molecular mechanism of graft union formation remains poorly understood.Here,cucumber grafted onto pumpkin was used to study graft union development,and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated.Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection,and the scion showed evident callus production compared with the rootstock 3 days after grafting.Consistently,transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development,the cell cycle,and sugar metabolism during both vascular reconnection and healing,indicating distinct mechanisms.Additionally,lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection.Next,we found that the healing process of grafted etiolated seedlings was significantly delayed,and graft success,xylem reconnection,and the growth of grafted plants were enhanced by exogenous glucose.This demonstrates that graft union formation requires the correct sugar content.Furthermore,we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin(TOR)inhibitor AZD-8055,and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment.Taken together,our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future. | Li Miao Qing Li Tian-shu Sun Sen Chai Changlin Wang Longqiang Bai Mintao Sun Yansu Li Xing Qin Zhonghua Zhang Xianchang Yu | 2021 | Horticulture Research2021，8，1： | 3 |
| 4 | FTIR spectroscopic characterization of soy proteins obtained through AOT reverse micelles显示文摘 | Xiangyan Chen Yi Ru Fengliang Chen Xianchang Wang Xiaoyan Zhao Qiang Ao | 2013 | Food Hydrocolloids2013，，2： | 2 |
| 5 | Preparation of water soluble Am–AA–SSS copolymers by inverse microemulsion polymerization显示文摘 | Tao Wan TianShun Zang YunCheng Wang Rui Zhang XianChang Sun | 2010 | Polymer Bulletin2010，，6： | 1 |
| 6 | Vibrational spectral investigation of four second order nonlinear optical azobenzenecontaining materials:A combination of experimental and density functional theoretical(DFT)study显示文摘 | Li Xianchang Li Wei Li Zhong’an | 2011 | Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy2011，79，5： | 1 |
| 7 | Improved fluid search optimization-based real-time weed mapping显示文摘In the field of agriculture,variable-rate herbicide spraying(VRHS)technology has been used to solve the low efficiency of pesticides and crop chemical residues.The key of VRHS is the quick and precise identification of weeds from field images,which forms a weed map.Fluid search optimization(FSO)was able to simplify the threshold optimization process to create a weed map,which simulated the fluid flowing from high pressure to low pressure,but it is time consuming and often converges prematurely.So,an explosion mechanism and a twophase optimization were introduced to improve the FSO-based segmentation algorithm.Experiments of segmentation weeds from a corn field at seedling growth stage showed that the IFSO algorithm obtained the best accuracy of 93.3%and the least running time of 0.019 s,compared with the standard PSO,GA,and FSO algorithms. | Chengcheng Chen Shengsheng Wang Xianchang Wang Helong Yu Ruyi Dong | 2020 | Information Processing in Agriculture2020，7，3： | 1 |
| 8 | Numerial analysis of the performance of a thermal ejector in a steam evaporator显示文摘 | Li Xianchang Wang Ting Day Benjamin | 2010 | Applied Thermal Engineering2010，30，17： | 1 |
| 9 | Combustion mechanism development and CFD simulation for the prediction of soot emission during flaring显示文摘 | Anan Wang Helen H. Lou Daniel Chen Anfeng Yu Wenyi Dang Xianchang Li Christopher Martin Vijaya Damodara Ajit Patki | 2016 | Frontiers of Chemical Science and Engineering2016，10，4： | 1 |
| 10 | Natural killer cells promote immune tolerance by regulating inflammatory TH17 cells at the human maternal-fetal interface显示文摘 | Binqing Fu Xianchang Li Rui Sun | 2012 | PNAS2012，，12： | 1 |
| 11 | Effect of low temperature stress on the protective-enzyme activity of grafted cucumber seedling and own-root cucumber seedling显示文摘 | Feng Xin Yu Xianchang Guo Hengjun | 2002 | Journal of Shan Dong Agricultural University(Natural Science)2002，33，3： | 1 |
| 12 | Propagation of gravity wave packet near critical level显示文摘A couple of two-dimensional linear and fully nonlinear numerical models for compressible atmosphere are used to numerically study the propagation of the gravity wave packet into a mean wind shear. For a linear propagation wave packet, the critical level interactions are in good agreement with the linear critical level theory. The dynamically and convectively unstable regions are formed due to the critical level interaction of a finite-amplitude wave packet, but they would not break. The free exchange of potential energy with kinetic energy in the background atmosphere at rest ceases after entering the mean wind shear. However, it still goes on in the nonlinear propagation. It is shown that the nonlinear effects modify the mean flow markedly, reduce the momentum and energy propagation velocity and drop the elevation of the critical level. The gravity wave packet becomes unstable and breaks down into smaller scales in some regions. It expends much more kinetic energy than potential energy in the early phase of the breakdown. This means that the wave breakdown sets up due to the action of the shear instability rather than a convective one. | YUE Xianchang YI Fan | 2005 | Science China(Technological Sciences)2005，48，5： | 1 |
| 13 | Joint observations of sporadic sodium and sporadic E layers at middle and low latitudes in China显示文摘Sporadic sodium layers(SSLs)were studied with sodium(Na)lidar at Beijing(40.5°N,116.0°E)and Haikou(19.5°N,109.1°E)in China.The altitude distribution,strength factors,and occurrence time of SSL and sporadic E(Es)layer events were statistically analyzed at both observing sites.SSL occurrence had a maximum near22:00 local time,and Eslayer occurrence had a maximum before midnight.The altitude distributions of SSL and Es layer events were better correlated at Haikou than at Beijing.All the average values of height and strength factor for SSL and Eslayer events,as well as the average of the maximum frequency that can be reflected by the Eslayer(foEs),at Beijing were higher than those at Haikou.A better correlation between SSL and Eslayer events was also found at Haikou,and the formation of SSLs was considered to likely depend on the seasonal variability of chemical and dynamical processes. | Jing Jiao Guotao Yang Xu Zou Shaohua Gong Jihong Wang Dawei Tian Wenzhen Zhang Tiemin Zhang Xianchang Yang Jun Fu Xuewu Cheng | 2014 | Chinese Science Bulletin2014，59，29： | 1 |
| 14 | Pre-treatment for diamond coatings on free-shape WC-Co tools显示文摘 | ZHAGN Zhiming HE Xianchang SHEN Hesheng | 2000 | Diamond and Related Materials2000，9，910： | 1 |
| 15 | Spectrometer-based combustion monitoring or flame stoichiometry and temperature control 显示文摘 | Romero Carlos Li Xianchang Keyvan Shahla | 2005 | Applied Thermal Engineering2005，25，56： | 1 |
| 16 | Thermal/structural analysis of radiator for heavyduty trucks显示文摘 | Mao Shaolin Cheng Changrui Li Xianchang | 2010 | Applied Thermal Engineering2010，，30： | 1 |
| 17 | Observation of the Double Sodium Layer Over Haikou,China by Lidar显示文摘A Double Sodium Layer(DSL) structure was observed during the night of August 22, 2011 over Haikou,China(20°N,110°E) by Na lidar.This DSL comprised a typical sodium layer at altitudes of 80~105km and a higher sodium layer at altitudes of 105~115km in about 0.5 h.A wavelength of 589 nm dye laser pumped by a Nd:YAG laser was used to make the measurement. The backscattered fluorescence photons from the sodium layer were collected by a telescope with a primary mirror of 1000 mm in diameter.The sodium density of these layers during the nighttime observation in the Mesosphere and Lower-Thermosphere(MLT) was studied. | ZHANG Tiemin WANG Jihong FU Jun YANG Dali YANG Xianchang WU Wei YANG Guotao | 2013 | 空间科学学报2013，33，4： | 1 |
| 18 | Effects of cold rolling on precipitates in inconel 718 显示文摘 | Li Rongbin He Xianchang Yao Mei | 2002 | Materials Engineering and Performance2002，11，5： | 1 |
| 19 | Fluoride passivation of ZnO electron transport layers for efcient PbSe colloidal quantum dot photovoltaics显示文摘Lead selenide(PbSe)colloidal quantum dots(CQDs)are suitable for the development of the next-generation of photovoltaics(PVs)because of efcient multiple-exciton generation and strong charge coupling ability.To date,the reported high-efcient PbSe CQD PVs use spin-coated zinc oxide(ZnO)as the electron transport layer(ETL).However,it is found that the surface defects of ZnO present a difculty in completion of passivation,and this impedes the continuous progress of devices.To address this disadvantage,fuoride(F)anions are employed for the surface passivation of ZnO through a chemical bath deposition method(CBD).The F-passivated ZnO ETL possesses decreased densities of oxygen vacancy and a favorable band alignment.Benefting from these improvements,PbSe CQD PVs report an efciency of 10.04%,comparatively 9.4%higher than that of devices using sol-gel(SG)ZnO as ETL.We are optimistic that this interface passivation strategy has great potential in the development of solution-processed CQD optoelectronic devices. | Jungang He You Ge Ya Wang Mohan Yuan Hang Xia Xingchen Zhang Xiao Chen Xia Wang Xianchang Zhou Kanghua Li Chao Chen Jiang Tang | 2023 | Frontiers of Optoelectronics2023，16，3： | 0 |
| 20 | Cross-immune tolerance:conception and its potential significance on transplantation tolerance显示文摘The diversity of alloreactive T cells in graft rejection and the presence of extensive crossreactivity among alloreactive T cells indicate thatthe induction oftransplantation tolerancemay fundamentally alter the size ofhost T-cell repertoireinvolved in protective immunity and immune surveillance,especially those that are crossreactive to conventional antigens.We herein highlight the crossreactive nature of alloreactive T cells and the potential risks of altered T-cell repertoire associated with the induction of transplantation tolerance.The possibility that T-cell tolerance to one set of antigens results in their tolerance to other unrelated antigens due to T-cell crossreactivity and/or heterogeneity is defined as‘cross-immune tolerance’.The definition and significance of this concept were discussed in details. | Yong Zhao Xianchang Li | 2010 | Cellular & Molecular Immunology2010，7，1： | 0 |
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