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1Integrated analysis of transcriptomic and proteomic data from tree peony(P.ostii)seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism显示文摘Tree peony(Paeonia section Moutan DC.)seeds are an excellent source of beneficial natural compounds that promote health,and they contain high levels of alpha-linolenic acid(ALA).In recent years,tree peony has been emerging as an oil crop.Therefore,combined analysis of the transcriptome and proteome of tree peony(P.ostii)seeds at 25,32,39,53,67,81,88,95,and 109 days after pollination(DAP)was conducted to better understand the transcriptional and translational regulation of seed development and oil biosynthesis.A total of 38,482 unigenes and 2841 proteins were identified.A total of 26,912 differentially expressed genes(DEGs)and 592 differentially expressed proteins(DEPs)were clustered into three groups corresponding to the rapid growth,seed inclusion enrichment and conversion,and late dehydration and mature stages of seed development.Fifteen lipid metabolism pathways were identified at both the transcriptome and proteome levels.Pathway enrichment analysis revealed that a period of rapid fatty acid biosynthesis occurred at 53–88 DAP.Furthermore,211 genes and 35 proteins associated with the fatty acid metabolism pathway,63 genes and 11 proteins associated with the biosynthesis of unsaturated fatty acids(UFAs),and 115 genes and 24 proteins associated with ALA metabolism were identified.Phylogenetic analysis revealed that 16 putative fatty acid desaturase(FAD)-encoding genes clustered into four FAD groups,eight of which exhibited the highest expression at 53 DAP,suggesting that they play an important role in ALA accumulation.RT-qPCR analysis indicated that the temporal expression patterns of oil biosynthesis genes were largely similar to the RNA-seq results.The expression patterns of fatty acid metabolism-and seed development-related proteins determined by MRM were also highly consistent with the results obtained in the proteomic analysis.Correlation analysis indicated significant differences in the number and abundance of DEGs and DEPs but a high level of consistency in expression patterns and metabolic pathways.The results of the present study represent the first combined transcriptomic and proteomic analysis of tree peony seeds and provide insight into tree peony seed development and oil accumulation.Xiaojing Wang Haiying Liang Dalong Guo Lili Guo Xiangguang Duan Qishi Jia Xiaogai Hou 2019Horticulture Research2019,6,1:9
2A novel PdNi/Al_2O_3 catalyst prepared by galvanic deposition for low temperature methane combustion显示文摘Galvanic deposition method was used to prepare the Pd/Ni-Al2O3-GD catalyst for the combustion of methane under lean conditions. The new catalyst and compared catalysts (Pd/Al2O3-IW, Pd-Ni/Al2O3-IW, Pd/Ni-Al2O3-IW) prepared by incipient wetness impregnation were characterized by N2-physisorption, XRD and TEM to clarify particle size and size distribution of palladium species. Combined O2-TPD and XPS results with the catalytic data, it shows that the surface palladium species with low valence exhibits better combustion performance due to their stronger interaction with support. The results indicate that the galvanic deposition method is an effective route to prepare efficient catalyst for methane combustion, and it also provides useful information for improving the present commercial catalyst.Xiqiang Pan Yibo Zhang Zhenzhen Miao Xiangguang Yang 2013Journal of Energy Chemistry2013,22,4:7
3A transient expression system in soybean mesophyll protoplasts reveals the formation of cytoplasmic GmCRY1 photobody-like structures显示文摘Soybean(Glycine max(L.) Merr.), grown for its plant oils and proteins, is one of the most important crops throughout the world.Generating stable and heritable transgenic soybeans is relatively inefficient;therefore, there is an urgent need for a simple and high-efficient transient transformation method by which to enable the investigation of gene functions in soybeans, which will facilitate the elucidation and improvement of the molecular mechanisms regulating the associated agronomic traits. We established a system of transient expression in soybean mesophyll protoplasts and obtained a high level of protoplast transfection efficiency(up to 83.5%). The subcellular activity of the protoplasts was well preserved, as demonstrated by the dynamic formation of GmCRY nucleus photobodies(NPs) and/or cytoplasmic photobody-like structures(CPs) in response to blue light.In addition, we showed that GmCRY1b CPs colocalized with GmCOP1b, a co-ortholog of Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC 1(COP1), which provided new insight into the potential roles of GmCRY1s in the cytoplasm.Lu Xiong Cong Li Hongyu Li Xiangguang Lyu Tao Zhao Jun Liu Zecheng Zuo Bin Liu 2019Science China(Life Sciences)2019,62,8:6
4Comparison of mixing layer height inversion algorithms using lidar and a pollution case study in Baoding, China显示文摘Beijing–Tianjin–Hebei area is suffering from atmospheric pollution from a long time. The understanding of the air pollution mechanism is of great importance for officials to design strategies for the environmental governance. Mixing layer height(MLH) is a key factor influencing the diffusion of air pollutants. It plays an important role on the evolution of heavy pollution events. Light detection and ranging(lidar), is an effective remote-sensing tool, which can retrieve high spatial and temporal evolution process within mixing layer(ML), especially the variation of MLH. There are many methods to retrieve MLH, but each method has its own applicable limitations. The Mie-lidar data in Beijing was firstly used to compare three different algorithms which are widely used under different pollution levels.We find that the multi-layer structure near surface may cause errors in the detection of mixing layer. The MLH retrieved based on image edge detection was better than another two methods especially under heavy polluted episode. Then we applied this method to investigate the evolution of the mixing layer height during a pollution episode in December2016. MLH at Gucheng county showed the positive correlation with the concentration of particulate matters during the start of this pollution episode. The elevated pollution level in Gucheng was not associated with MLH's decrease, and the significantly increased particulate matters raised the boundary layer, which trapped the pollutants near the surface.Xiangguang Ji Cheng Liu Zhouqing Xie Qihou Hu Yunsheng Dong Guangqiang Fan Tianshu Zhang Chengzhi Xing Zhuang Wang Zeeshan Javed Jianguo Liu 2019Journal of Environmental Sciences2019,31,5:5
5Precipitation transformation of rare earth sulfate into chloride with p-dodecylphenoxy carboxylic acids显示文摘Methods for transforming rare earth(RE)sulfate into chloride mainly include extraction process with organophosphonic mono-acids or aliphatic acids and precipitation process with ammonium bicarbonate(NH4 HCO3).In this paper,alkylphenoxy carboxylic acids(HAs)ofp-dodecylphenoxy acetic acid(HA-Ⅰ),pdodecylphenoxypropanoic acid(HA-Ⅱ)and p-dodecylphenoxybutyric acid(HA-Ⅲ),which were liquid at room temperature were synthesized and characterized.The precipitation mechanisms of RE elements with the HAs were investigated and the HA/RE molar ratios of the solid complexes were determined as3:1 by equi-molar series method which accord with the principle of charge balance.Applicability of HAs for the transformation of RE sulfate from concentrated sulfuric acid roasted RE concentrate into chloride via precipitation method was discussed.100%HA-Ⅱwas selected as the liquid organic precipitant without dilution of volatile solvent soracceleration of phase separation by phase-modifiers.The RE sulfate solution can be precipitated by HA-Ⅱafter neutralization with liquid NaOH and stripped with concentrated HCl at room temperature.High concentration of RE chloride of 218.1 g/L with low residue of sulfate radical of 0.536 g/L was obtained.The residual organic precipitant in the raffinate solution was tested to be lower than 8 mg/L at 25℃and the chemical oxygen demand(COD)in wastewater was less than 50 mg/L.Yanliang Wang Jia Su Xiangguang Guo Jianguo Cui Yan Bai Fujian Li Xiaoqi Sun 2019Journal of Rare Earths2019,37,12:5
6Novel resonant pressure sensor based on piezoresistive detection and symmetrical in-plane mode vibration显示文摘In this paper,a novel resonant pressure sensor is developed based on electrostatic excitation and piezoresistive detection.The measured pressure applied to the diaphragm will cause the resonant frequency shift of the resonator.The working mode stress–frequency theory of a double-ended tuning fork with an enhanced coupling beam is proposed,which is compatible with the simulation and experiment.A unique piezoresistive detection method based on small axially deformed beams with a resonant status is proposed,and other adjacent mode outputs are easily shielded.According to the structure design,high-vacuum wafer-level packaging with different doping in the anodic bonding interface is fabricated to ensure the high quality of the resonator.The pressure sensor chip is fabricated by dry/wet etching,high-temperature silicon bonding,ion implantation,and wafer-level anodic bonding.The results show that the fabricated sensor has a measuring sensitivity of~19 Hz/kPa and a nonlinearity of 0.02%full scale in the pressure range of 0–200 kPa at a full temperature range of−40 to 80°C.The sensor also shows a good quality factor>25,000,which demonstrates the good vacuum performance.Thus,the feasibility of the design is a commendable solution for high-accuracy pressure measurements.Xiangguang Han Qi Mao Libo Zhao Xuejiao Li Li Wang Ping Yang Dejiang Lu Yonglu Wang Xin Yan Songli Wang Nan Zhu Zhuangde Jiang 2020Microsystems & Nanoengineering2020,6,1:4
7Effect of strontium substitution on the activity of La_(2-x)Sr_xNiO_4(x=0.0-1.2)in NO decomposition显示文摘The aim of this work is to study the effect of Sr substitution on the redox properties and catalytic ac- tivity of La2-xSrxNiO4(x=0.0-1.2)for NO decomposition.Results suggest that the x=0.6 sample shows the highest activity.The characterization(TPD,TPR,etc.)of samples indicates that the x=0.6 sample possesses suitable abilities in both oxidation and reduction,which facilitates the proceeding of oxygen desorption and NO adsorption.At temperature below 700℃,the oxygen desorption is difficult, and is the rate-determining step of NO decomposition.With the increase of reaction temperature(T> 700℃),the oxygen desorption is favorable and,the active adsorption of NO on the active site(NO+Vo+ Ni2+→NO^--Ni3+)turns out to be the rate-determining step.The existence of oxygen vacancy is the prerequisite condition for NO decomposition,but its quantity does not relate much to the activity.ZHU JunJiang 1 ,YANG XiangGuang 2 ,XU XueLian 3 &WEI KeMei 1 1 National Research Center of Chemical Fertilizer Catalysts,Fuzhou University,Fuzhou 350002,China 2 Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China 3College of Chemistry&Chemical Engineering,Fuzhou University,Fuzhou 350002,China 2007Science China Chemistry2007,50,1:4
8GmCRY1s modulate gibberellin metabolism to regulate soybean shade avoidance in response to reduced blue light显示文摘Soybean is an important legume crop that displays the classic shade avoidance syndrome(SAS),including exaggerated stem elongation,which leads to lodging and yield reduction under density farming conditions.Here,we compared the effects of two shade signals,low red light to far-red light ratio(R:FR)and low blue light(LBL),on soybean status and revealed that LBL predominantly induces excessive stem elongation.We used CRISPR-Cas9-engineered Gmcry mutants to investigate the functions of seven cryptochromes(GmCRYs)in soybean and found that the four GmCRY1s overlap in mediating LBL-induced SAS.Lightactivated GmCRY1s increase the abundance of the bZlP transcription factors STF1 and STF2,which directly upregulate the expression of genes encoding GA2 oxidases to deactivate GA1 and repress stem elongation.Notably,GmCRY1b overexpression lines displayed multiple agronomic advantages over the wild-type control under both dense planting and intercropping conditions.Our study demonstrates the integration of GmCRY1-mediated signals with the GA metabolic pathway in the regulation of LBL-induced SAS in soybean.It also provides a promising option for breeding lodging-resistant,high-yield soybean cultivars in the future.Xiangguang Lyu Qican Cheng Chao Qin Yinghui Li Xinying Xu Ronghuan Ji Ruolan Mu Hongyu Li Tao Zhao Jun Liu Yonggang Zhou Haiyan Li Guodong Yang Qingshan Chen Bin Liu 2021Molecular Plant2021,14,2:4
9The development of a new real-time subsurface mooring显示文摘Subsurface mooring allows researchers to measure the ocean properties such as water temperature,salinity,and velocity at several depths of the water column for a long period.Traditional subsurface mooring can release data only after recovered,which constrains the usage of the subsurface and deep layer data in the ocean and climate predictions.Recently,we developed a new real-time subsurface mooring(RTSM).Velocity profiles over upper 1000 m depth and layered data from sensors up to 5000 m depth can be realtime transmitted to the small surface buoy through underwater acoustic communication and then to the office through Beidou or Iridium satellite.To verify and refine their design and data transmission process,we deployed more than 30 sets of RTSMs in the western Pacific to do a 1-year continuous run during 2016–2018.The continuous running period of RTSM in a 1-year cycle can reach more than 260 days on average,and more than 95%of observed data can be successfully transmitted back to the office.Compared to the widely-used inductive coupling communication,wireless acoustic communication has been shown more applicable to the underwater sensor network with large depth intervals and long transmission distance to the surface.WANG Fan WANG Jianing XU Lijun ZHANG Xiangguang YAN Shefeng CHEN Yonghua 2020Journal of Oceanology and Limnology2020,38,4:2
10Validation results of NmF2 and hmF2 derived from ionospheric density profiles of GNOS on FY-3C satellite显示文摘The first Global Navigation Satellite System Occultation Sounder(GNOS) which is compatible of both Bei Dou System(BDS)and Global Positioning System(GPS) was successfully launched into orbit onboard the Feng Yun 3 C satellite(FY-3 C) on September 23, 2013, and it has already gathered a large amount of ionosphere radio occultation(RO) data so far. However, the detailed analysis and validation of GPS ionosphere RO data have not been done up to now. Therefore, this paper discusses the configuration of the FY-3 C GNOS, the methods and results of GPS ionosphere occultation processment, the quality analysis of the GPS ionosphere RO products, and the precision consistency between the GNOS GPS ionosphere RO product and ionosonde data. The peak electron density(Nm F2) correlation coefficient is 0.97, the corresponding standard deviation is 16.08%. The peak value altitude(hm F2) correlation coefficient is 0.89, and the corresponding standard deviation is 23.79 km. The in-orbit operation of GNOS provides a basis data set for the monitoring, forecasting and research of ionosphere's space weather.YANG GuangLin SUN YueQiang BAI WeiHua ZHANG XiaoXin LIU CongLiang MENG XiangGuang BI YanMeng WANG DongWei ZHAO DanYang 2018Science China(Technological Sciences)2018,61,9:2
11Recovery of thorium and rare earths from leachate of ion-absorbed rare earth radioactive residues with N1923 and Cyanex■572显示文摘Ion-absorbed rare earth ores radioactive residues(IREORR)are a class of waste residue from the production of rare earth elements(REEs).Because of its radioactive dose,IREORR are usually stored in waste warehouses.IREORR are difficult to be disposed of.However,it contains relatively high concentrations of REEs,which can be considered as a valuable secondary resource.In this paper,a novel process is developed for the separation of thorium(Th)and recovery of REEs from IREORR hydrochloric acid leachate with primary amine N1923 and Cyanex?572,respectively.The effects of sulfate concentration,extractant concentration and pH on N1923 extraction in hydrochloric acid solution were investigated in detail.The results show that the extraction capacity of N1923 can be improved by adding sulfate to the solution and increasing the concentration of N1923.Acidity has little effect on the extraction of Th when pH is higher than 1.As for the stripping,REEs are more easily stripped from loaded organic phase than Th,and nitric acid is a better stripping agent than hydrochloric acid.Combined with the extraction of Cyanex■572 for REEs,a fractional extraction experiment for separating Th and enriching of REEs was performed.The yield of Th is higher than 99.9%and the concentration of REEs is enriched to 183.84 g/L.Jia Su Xiangguang Guo Yun Gao Shan Wu Ruigao Xu Xiaoqi Sun 2021Journal of Rare Earths2021,39,10:2
12A Novel Nano-sized Catalyst CeO2-CuO/Hollow ZSM-5 for NOx Reduction with NH3显示文摘YI Ting LI Jingwei ZHANG Yibo YANG Xiangguang 2018Chemical Research in Chinese Universities2018,34,4:2
13Immobilization of the heteropoly acid (HPA) H4 SiW12O40 (SiW12) on mesoporous molecular sieves (HMS and MCM-41) and their catalytic behavior 显示文摘Chu Wenling Yang Xiangguang Shan Yongkui 1996Catalysis Letters1996,42,:1
14Brominelpara-Toluenesulfonic Acid-Catalyzed Synthesis of 3,3-Bis(indole-3-yl)indoline-2-(1H)-ones by Condensing Indoles with Isatins显示文摘在仅仅在房间温度的 Br 2 的 3 mol% 的催化作用下面,吲哚与靛红很快反应了形成生物学上重要的 3,3 二度(indole-3-yl ) 有高效率和宽底层范围的 indoline-2-(1H ) 。而且,我们证明 p-toluenesulfonic 酸(TsOH ) 能用作一个代理人催化这转变。Wenzhong Huang Lingli Nang Xiangguang Li Lin Yuan Yinhai Ma Deqiang Liang 2015Chinese Journal of Chemistry2015,33,10:1
15Layered La-Ba-Cu mixed oxides with perovskite structure as catalyst for NO reduction by CO显示文摘The mixed oxides, including LaBa\-2Cu\-3O\-7, LaBaCu\-2O\-5, La\-4BaCu\-5O 12 with perovskite structure, were prepared. The catalysts were characterized by means of chemical analysis, XRD, H\-2_TPR. It was found that their structures were layered ABO\-3 perovskite structure and they were the active catalysts for the NO reduction by CO. The existance of Cu 3+ is an important factor to give the catalysts a high activity for the NO reduction by CO.Yu Liu Xiangguang Yang Zhen Zhao Yue Wu 1998Chinese Science Bulletin1998,43,1:1
16Direct decomposition of NO over the mixed catalysts Nd2-xSrxNiO4 + &显示文摘Zhao Zhen Yang Xiangguang Wu Yue 1996Chinese Science Bulletin1996,41,11:1
17Cryptochrome 1 Inhibits Shoot Branching by Repressing the Self-Activated Transciption Loop of PIF4 in Arabidopsis显示文摘Cryptochrome 1(CRY1)is an important light receptor essential for de-etiolation of Arabidopsis seedlings.However,its function in regulating plant architecture remains unclear.Here,we show that mutation in CRY1 resulted in increased branching of Arabidopsis plants.To investigate the underlying mechanism,we analyzed the expression profiles of branching-related genes and found that the mRNA levels of Phytochrome Interaction Factor 4(PIF4)and PIF5 are significantly increased in the cry1 mutant.Genetic analysis showed that the pif4 or pif4pif5 mutant is epistatic to the cry1 mutant,and overexpression of PIF4 conferred increased branching.Moreover,we demonstrated that PIF4 proteins physically associate with the G-box motif within the PIF4 promoter to form a self-activated transcriptional feedback loop,while CRY1 represses this process in response to blue light.Taken together,this study suggests that the CRY1–PIF4 module regulates gene expression via forming a regulatory loop and shoot branching in response to ambient light conditions.Huawei Zhai Lu Xiong Hongyu Li Xiangguang Lyu Guodong Yang Tao Zhao Jun Liu Bin Liu 2020Plant Communications2020,1,3:1
18One-Pot Three-Dimensional Printing Robust Self-Supporting MnO_(x)/Cu-SSZ-13 Zeolite Monolithic Catalysts for NH_(3)-SCR显示文摘Honeycomb cordierite coated with Cu-SSZ-13 zeolite is widely used for the selective catalytic reduction of NO_(x) with NH_(3)(NH_(3)-SCR)to reduce pollutants from vehicle emissions.However,conventional honeycomb catalysts fabricated via coating techniques are limited by low zeolite loadings,loss of the deposited zeolites,and complicated preparation processes.Herein,a facile,one-step three-dimensional(3D)printing strategy is developed to construct MnO_(x)/Cu-SSZ-13 monolithic catalysts with excellent catalytic performance for NH_(3)-SCR.Iron-containing halloysite nanotubes(Fe-HNTs)are introduced as printing ink additives to ensure mechanical stability and modulate the NH_(3)-SCR performance of monolithic catalysts in high temperature conditions.In situ incorporation of Mn into the Cu-SSZ-13 zeolite monoliths during the 3D printing process boosts the mechanical strength of the monolithic structures from 2.54 MPa to 4.33 MPa as well as broadens the temperature window(165-550℃)of the catalysts for NH_(3)-SCR with NO_(x) conversion of above 80%.Such robust multicomponent-integrated 3D-printed selfsupporting catalysts not only possess high zeolite loading and excellent catalytic activity,but also avoid complicated manufacturing processes,which contrasts with conventional honeycomb catalysts fabricated by extrusion coupled with coating.Yingzhen Wei Mengyang Chen Xiaoyu Ren Qifei Wang Jinfeng Han Wenzheng Wu Xiangguang Yang Shuang Wang Jihong Yu 2022CCS Chemistry2022,4,5:1
19CRISPR/Cas9-engineered mutation to identify the roles of phytochromes in regulating photomorphogenesis and flowering time in soybean显示文摘Soybean(Glycine max)responds to ambient light variation by undergoing multiform morphological alterations,influencing its yield potential and stability in the field.Phytochromes(PHYs)are plant-specific red(R)and far-red(FR)light photoreceptors mediating photomorphogenesis and photoperiodic flowering.As an ancient tetraploid,soybean harbors four PHYA,two PHYB,and two PHYE paralogs.Except for GmPHYA2/E4 and GmPHYA3/E3,which have been identified as photoperiod-dependent flowering repressors,the functions of GmPHYs are still largely unclear.We generated a series of individual or combined mutations targeting the GmPHYA or GmPHYB genes using CRISPR/Cas9 technology.Phenotypic analysis revealed that GmPHYB1 mediates predominantly R-light induced photomorphogenesis,whereas GmPHYA2/E4 and GmPHYA3/E3,followed by GmPHYA1 and GmPHYB2,function redundantly and additively in mediating FR light responses in seedling stage.GmPHYA2/E4 and GmPHYA3/E3,with weak influence from GmPHYA1 and GmPHYA4,delay flowering time under natural long-day conditions.This study has demonstrated the diversified functions of GmPHYAs and GmPHYBs in regulating light response,and provides a core set of phytochrome mutant alleles for characterization of their functional mechanisms in regulating agronomic traits of soybean.Fen Zhao Xiangguang Lyu Ronghuan Ji Jun Liu Tao Zhao Hongyu Li Bin Liu Yanxi Pei 2022The Crop Journal2022,10,6:1
20En- hanced Performance in Vapor o-Methylation of Hydroxyben- zene over a Noval Kind of Mesoporous Rare Earth Phosphate 显示文摘Zhang Zhen Liu Qingbo Yang Xiangguang 2010ZPhys Chem2010,224,6:1
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