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| 1 | Evolutionary conservation of Dmrt gene family in amphibi-ans, reptiles and birds显示文摘Sex determining gene Mab-3 of C. elegans and doublesex of Drosophila contain a common DNA binding motif called a DM domain, both of which regulate similar aspects of sexual development. Human doublesex-related gene DMRT1 has been identified, which also contains the conserved DM-related DNA-binding domain and plays an essential role in gonadal differentiation. We present the amplification of a broad spectrum of DM domain sequences from phylogenetic diverse vertebrates (Cynops orientalis, Chrysemys scripta elegans and Coturnix coturnix) using degenerate PCR. Our results further reveal the unexpected complexity and the evolutionary conservation of the DM domain gene family. | REN Lili CHENG Hanhua GUO Yiqing HUANG Xiao LIU Li ZHOU Rongjia | 2001 | Chinese Science Bulletin2001，46，23： | 40 |
| 2 | Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4显示文摘最近的尖铁 glycoprotein (S) 鉴别中东呼吸症候群 coronavirus (MERS-CoV ) 指向细胞的受体, dipeptidyl peptidase 4 (DPP4 ) 。顺序比较和当模特儿的分析在病毒的尖铁上揭示了一个通常认为的受体绑定领域(RBD ) ,它调停这个相互作用。我们报导 3.0 Å MERS-CoV RBD 的决定水晶结构跳了到人的 DPP4 的细胞外的领域。我们的结果证明 MERS-CoV RBD 由一个核心和受体绑定子域组成。受体绑定子域与 DPP4 β 交往;推进器然而并非它的内在的 hydrolase 领域。MERS-CoV RBD 和相关 SARS-CoV RBD 分享他们的核心子域的结构的类似的高度,但是在受体绑定子域是尤其是分叉的。Mutagenesis 研究在为到 DPP4 和入口的病毒的绑定是批评的进目标房间的受体绑定子域识别了几关键残余。在在 MERS-CoV RBD 和 DPP4 之间的接口的原子细节提供病毒和受体相互作用的结构的理解,它能对 MERS-CoV 感染指导治疗学和疫苗的开发。 | Nianshuang Wang Xuanling Shi Liwei Jiang Senyan Zhang Dongli Wang Pei Tong Dongxing Guo Lili Fu Ye Cui Xi Liu Kelly C Arledge Ying-Hua Chen Linqi Zhang Xinquan Wang | 2013 | Cell Research2013，23，8： | 26 |
| 3 | Characterization of drug responses of mini patient-derived xenografts in mice for predicting cancer patient clinical therapeutic response显示文摘Background:Patient-derived organoids and xenografts(PDXs)have emerged as powerful models in functional diag-nostics with high predictive power for anticancer drug response.However,limitations such as engraftment failure and time-consuming for establishing and expanding PDX models followed by testing drug efficacy,and inability to subject to systemic drug administration for ex vivo organoid culture hinder realistic and fast decision-making in selecting the right therapeutics in the clinic.The present study aimed to develop an advanced PDX model,namely MiniPDX,for rapidly testing drug efficacy to strengthen its value in personalized cancer treatment.Methods:We developed a rapid in vivo drug sensitivity assay,OncoVee®MiniPDX,for screening clinically relevant regimens for cancer.In this model,patient-derived tumor cells were arrayed within hollow fiber capsules,implanted subcutaneously into mice and cultured for 7 days.The cellular activity morphology and pharmacokinetics were systematically evaluated.MiniPDX performance(sensitivity,specificity,positive and negative predictive values)was examined using PDX as the reference.Drug responses were examined by tumor cell growth inhibition rate and tumor growth inhibition rate in PDX models and MiniPDX assays respectively.The results from MiniPDX were also used to evaluate its predictive power for clinical outcomes.Results:Morphological and histopathological features of tumor cells within the MiniPDX capsules matched those both in PDX models and in original tumors.Drug responses in the PDX tumor graft assays correlated well with those in the corresponding MiniPDX assays using 26 PDX models generated from patients,including 14 gastric cancer,10 lung cancer and 2 pancreatic cancer.The positive predictive value of MiniPDX was 92%,and the negative predictive value was 81%with a sensitivity of 80%and a specificity of 93%.Through expanding to clinical tumor samples,Min-iPDX assay showed potential of wide clinical application.Conclusions:Fast in vivo MiniPDX assay based on capsule implantation was developed-to assess drug responses of both PDX tumor grafts and clinical cancer specimens.The high correlation between drug responses of paired MiniPDX and PDX tumor graft assay,as well as translational data suggest that MiniPDX assay is an advanced tool for personalized cancer treatment. | Feifei Zhang Wenjie Wang Yuan Long Hui Liu Jijun Cheng Lin Guo Rongyu Li Chao Meng Shan Yu Qingchuan Zhao Shun Lu Lili Wang Haitao Wang Danyi Wen | 2018 | Cancer Communications2018，38，1： | 20 |
| 4 | PM_(2.5), PM_(10) and health risk assessment of heavy metals in a typical printed circuit noards manufacturing workshop显示文摘A typical Printed Circuit Board(PCB) manufacturer was chosen as the object of this study.During PCB processing, fine particulate matter and heavy metals(Cu, Zn, Pb, Cr, Cd and Ni)will be released into the air and dust, which then impact workers' health and the environment. The concentrations of total suspended particle(TSP), PM10 and PM2.5in the off-site were 106.3, 90.0 and 50.2 μg/m3, respectively, while the concentrations of TSP, PM10 and PM2.5in the workshops ranged from 36.1 to 365.3, from 27.1 to 289.8 and from 22.1 to212.3 μg/m3, respectively. Almost all six of the heavy metals were detected in all of the particle samples except Cd. For each workshop, it was obvious that Zn was the most enriched metal in TSP, followed by Cu > Pb(Cr) > Ni > Cd, and the same trend was found for PM10 and PM2.5. In the dust samples, Cu(which ranged from 4.02 to 56.31 mg/g) was the most enriched metal, followed by Zn, Cr, Pb, Ni and Cd, and the corresponding concentrations ranged from 0.77 to 4.47, 0.37 to 1.59, 0.26 to 0.84, 0.13 to 0.44 and nd to0.078 mg/g, respectively. The health risk assessment showed that noncancerous effects are unlikely for Zn, Pb, Cr, Cu, Cd and Ni. The carcinogenic risks for Cd and Ni were all lower than 10-6, except for Cr. This result indicates that carcinogenic risks for workers are relatively possible in the workshops. These findings suggest that this technology is advanced from the perspective of environmental protection in the waste PCB's recycling industry. | Peng Zhou Jie Guo Xiaoyu Zhou Wei Zhang Lili Liu Yangcheng Liu Kuangfei Lin | 2014 | Journal of Environmental Sciences2014，26，10： | 18 |
| 5 | Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application显示文摘The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application.Early angiogenesis is important for scaffold survival.It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties.In this study,a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold.For osseointegration and osteogenesis analysis,in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation,adhesion,extracellular matrix(ECM)mineralization and ALP activity compared with bare Ti6Al4V cocultivation.Additionally,MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesisrelated genes expression.In vivo studies including fluorochrome labeling,micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation.For angiogenesis studies,magnesium-coated Ti6Al4V improve HUVECs proliferation,adhesion,tube formation,wound-healing and Transwell abilities.HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes(HIF-1αand VEGF)expression.Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation.This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications. | Peng Gao Bo Fan Xiaoming Yu Wenwen Liu Jie Wu Lei Shi Di Yang Lili Tan Peng Wan Yulin Hao Shujun Li Wentao Hou Ke Yang Xiaokang Li Zheng Guo | 2020 | Bioactive Materials2020，5，3： | 15 |
| 6 | Influence of rolling parameters on dynamically recrystallized microstructures in AZ31 magnesium alloy sheets显示文摘Conventional rolling experiments via the embedded pin in rolling sheet method were carried out at different reduction rates,starting rolling temperatures,and rolling speeds,and the effects of rolling parameters(i.e.,temperature,equivalent strain,and rolling time)on dynamically recrystallized(DRX)microstructures of AZ31 alloy during hot rolling were studied quantitatively.The temperature-strain dependence of the high-angle grain boundary fraction(HAGB%)was examined through electron backscattered diffraction.Results showed that as-rolled microstructures with high HAGB%may be obtained under average rolling temperatures of 270-320℃,equivalent strains higher than 0.8,and a rolling speed of 246 mm/s.These results may be related to the DRX kinetics and dynamic recovery which are controlled by deformation temperature and strain.HAGB%decreased with increasing rolling time(decreasing rolling speed),which is attributed to dynamic recovery,and the recrystallized grain size decreased as rolling time increased.However,further increases in rolling time increased average grain sizes but decreased mean subgrain sizes;these results are attributed to increases in the low-angle grain boundary(LAGB)length per unit area with rolling time.LAGB formation was controlled by dynamic recovery,which consistently follows polygonization or formation of new subgrains inside larger grains;hence,average subgrain sizes decreased with the rolling time.The effect of dynamic recovery on HAGB and LAGB formation and their related mechanisms over a wide range of strains and temperatures were discussed in detail. | Lili Guo Fumio Fujita | 2015 | Journal of Magnesium and Alloys2015，3，2： | 11 |
| 7 | Efficacy and safety of Shaoyang Xibi decoction in patients with knee osteoarthritis:a multi-center,single-blind,randomized controlled trial显示文摘PURPOSE: To observe the efficacy and safety of Shaoyang Xibi decoction(SYXBD) in patients with knee osteoarthritis(KOA), and to verify that the theory of 'Shaoyang dominating bone' in Traditional Chinese Medicine(TCM) can be applied to KOA treatment.METHODS: Participants were randomly allocated to two groups: SYXBD(treatment group, n = 66)and Meloxicam(control group, n = 66). Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC) and 36-Item Short Form Health Survey(SF-36) were used to assess efficacy before the treatment and 8 weeks after the treatment.RESULTS: Baseline data before the treatment between the two groups were similar. The WOMAC scores significantly decreased and the SF-36 scores significantly increased after 8-week treatment in both groups compared with before the treatment(P < 0.05). SYXBD significantly decreased pain scores(P < 0.001), physical function scores(P <0.001) and the total scores(P < 0.001) in WOMAC compared to Meloxicam. SYXBD significantly improved physical function(P = 0.021), bodily pain(P = 0.002) and general health(P = 0.014), with no significant difference in role emotional(P = 0.053),role physical(P = 0.517), vitality(P = 0.241), social function(P = 0.712) and mental health(P = 0.800)in SF-36 compared to Meloxicam. No adverse events were reported in the treatment group while13 adverse events happened in the control group during the study.CONCLUSION: SYXBD, prepared based on the theory of 'Shaoyang dominating bone', has a better curative efficay and safety in patients with KOA compared with Meloxicam. The TCM theory of 'Shaoyang dominating bone' may be useful in KOA treatment. | Si Yuhao Ma Yong Guo Yang Pan Yalan Zheng Suyang Xu Guihua Da Weiwei Xu Lili | 2018 | Journal of Traditional Chinese Medicine2018，38，5： | 11 |
| 8 | Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer显示文摘MicroRNAs (miRNAs ) 玩在在各种各样的癌症的发展和前进的关键角色。不正常的 miR-9 表示仍然保持模糊,并且卵巢的癌症的变形前进上的一致都没被到达。在这研究,从生物信息学分析的结果证明 E-cadherin mRNA 的 3-UTR 被 miR-9 直接调整。酶记者试金结果证实 miR-9 能直接指向这 3-UTR。在卵巢的癌症织物的 miR-9 和 E-cadherin 表示被 qRT-PCR 确定。移植和侵略被在 SKOV3 和 A2780 愈合弯屈和 Transwell 系统试金检测。qRT-PCR 和西方的污点被执行检测上皮 ? 间充质的联系转变的 mRNA 和蛋白质。Immunofluorescence 技术被用来分析表示和 E-cadherin, N-cadherin,和 vimentin 的 subcellular 本地化。结果证明 miR-9 经常与配对的主要的相比是在变形浆液的卵巢的癌症织物的 upregulated。miR-9 的 Upregulation 能 downregulate E-cadherin 的表示但是 upregulate 间充质的标记(N-cadherin 和 vimentin ) 的表示。miR-9 的 Overexpression 能在卵巢的癌症支持房间移植和侵略,并且这些进程能有效地经由 miR-9 禁止者被禁止。因此,我们的学习证明 miR-9 可以经由指向 E-cadherin 和一条新奇潜在的治疗学的途径控制卵巢的癌症的转移支持卵巢的癌症转移。 | Bo Zhou Hongbin Xu Meng Xia Chaoyang Sun Na Li Ensong Guo Lili Guo Wanying Shan Hao Lu Yifan Wu Yuan Li Degui Yang Danhui Weng Li Meng Junbo Hu Ding Ma Gang Chen Kezhen Li | 2017 | Frontiers of Medicine2017，11，2： | 10 |
| 9 | Microstructure,texture evolution and mechanical properties of cold rolled Ti-32.5Nb-6.8Zr-2.7Sn biomedical beta titanium alloy显示文摘Ti-32.5 Nb-6.8 Zr-2.7 Sn(TNZS,wt%) alloy was produced by using vacuum arc melting method,followed by solution treatment and cold rolling with the area reductions of 50% and 90%.The effects of cold rolling on the microstructure,texture evolution and mechanical properties of the experimental alloy were investigated by optical microscopy,X-ray diffraction,transmission electron microscopy and universal material testing machine.The results showed that the grains of the alloy were elongated along rolling direction and stress-induced α'' martensite was not detected in the deformed samples.The plastic deformation mechanisms of the alloy were related to {112} 111 type deformation twinning and dislocation slipping.Meanwhile,the transition from γ-fiber texture to α-fiber texture took place during cold rolling and a dominant {001} 110_(α-fiber) texture was obtained after 90% cold deformation.With the increase of cold deformation degree,the strength increased owing to the increase of microstrain,dislocation density and grain refinement,and the elastic modulus decreased owing to the increase of dislocation density as well as an enhanced intensity of {001} 110_(α-fiber)texture and a weakened intensity of {111} 112_(γ-fiber)texture.The 90% cold rolled alloy exhibited a great potential to become a new candidate for biomedical applications,since it possesses low elastic modulus(47.1 GPa),moderate strength(883 MPa) and high elastic admissible strain(1.87%),which are superior than those of Ti-6 Al-4 V alloy. | Chunbo Lan Yu Wu Lili Guo Huijuan Chen Feng Chen | 2018 | Journal of Materials Science & Technology2018，34，5： | 10 |
| 10 | Characteristics of fecal microbiota in non-alcoholic fatty liver disease patients显示文摘This study was designed to investigate the gut microbiota of patients with non-alcoholic fatty liver disease. The inclusive and exclusive criteria for NAFLD patients and healthy subjects were formulated, and detailed clinical data were collected. The genomic DNA of stool samples were extracted for 16 S r DNA sequencing, and the amplified V4-region was sequenced on the Illumina Miseq platform. Metastats analysis was performed to identify the differential taxa between the groups. Redundancy analysis was used to evaluate the association between gut microbial structure and clinical variables. Thirty NAFLD patients and37 healthy controls were involved. The 16 S r DNA sequencing showed that there was a dramatic variability of the fecal microbiota among all the individuals. Metastats analysis identified eight families and 12 genera with significant differences between the two groups. When some clinical parameters, such as waist-to-hip ratio(WHR) and homeostasis model assessment of insulin resistance(HOMA-IR), were enrolled in Redundancy analysis, the distribution of the two group of samples was obviously changed. The compositional shifts in fecal bacterial communities of NAFLD patients from the healthy controls were mainly at family or genus levels. According to our Redundancy analysis, insulin resistance and obesity might be closely related to both NAFLD phenotype and intestinal microecology. | Fan Li Gang Sun Zikai Wang Wenming Wu He Guo Lihua Peng Lili Wu Xu Guo Yunsheng Yang | 2018 | Science China(Life Sciences)2018，61，7： | 9 |
| 11 | Improving the accuracy of image-based forest fire recognition and spatial positioning显示文摘Forest fires are frequent natural disasters.It is necessary to explore advanced means to monitor,recognize and locate forest fires so as to establish a scientific system for the early detection,real-time positioning and quick fighting of forest fires.This paper mainly expounds methods and algorithms for improving accuracy and removing uncertainty in image-based forest fire recognition and spatial positioning.Firstly,we discuss a method of forest fire recognition in visible-light imagery.There are four aspects to improve accuracy and remove uncertainty in fire recognition:(1)eliminating factors of interference such as road and sky with high brightness,red leaves,other colored objects and objects that are lit up at night,(2)excluding imaging for specific periods and azimuth angles for which interference phenomena repeatedly occur,(3)improving the thresholding method for determining the flame border in image processing by adjusting the threshold to the season,weather and region,and (4)integrating the visible-light image method with infrared image technology.Secondly,we examine infrared-image-based methods and approaches of improving the accuracy of forest fire recognition by combining the spectrum threshold with an object feature value such as the normalized difference vegetation index and excluding factors of disturbance such as interference signals,extreme weather and high-temperature animals.Thirdly,a method of visible analysis to enhance the accuracy of forest fire positioning is examined and realized;the method includes decreasing the visual angle,selecting central points,selecting the largest spots,and judging the selection of fire spots according to the central distance.Case studies are examined and the results are found to be satisfactory. | JIANG LiLi 1,QI QingWen 1,ZHANG An 1,GUO ChaoHui 2 & CHENG Xi 1,3 1 Institute of Geographical Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing 100101,China 2 China Center for Resources Satellite Data and Applications,Beijing 100094,China 3Graduate University of Chinese Academy of Sciences,Beijing 100049,China | 2010 | Science China(Technological Sciences)2010，53，S1： | 9 |
| 12 | Breeding strategy of Acrossocheilus fasciatus in the Puxi Stream of the Huangshan Mountain显示文摘Breeding strategies of Acrossocheilus fasciatus were studied from 592 specimens collected monthly during May 2007 and April 2008 in the Puxi Stream of the Huangshan Mountain.Sex ratio of the studied population was 0.90∶1(♀∶♂),not significantly different from 1∶1.Both sexes reached their first sexual maturity at age 2(the second calendar year of their birth).Fifty percent of females and males reached maturity at a total length of 69.75 mm and 69.36 mm respectively,and the minimum total length was 61.54 mm and 58.96 mm,respectively.Based on the monthly changes in gonado-somatic index and egg-development process,the breeding season of the population was from May to August,with one obvious interval(in June)occurring in the breeding activity for the females.The nonsynchronous development of oocytes observed in mature ovaries indicated that A.fasciatus is a batch spawner.Absolute fecundity of A.fasciatus ranged from 308 to 2002 eggs with a mean of 857 eggs,increased significantly with total length,and was significantly different among three age groups.Relative fecundity ranged from 38.63 to 71.70 egg/g with a mean of 53.29 egg/g,and was not significantly different among the three age groups.It was suggested that these reproductive characteristics were adaptive strategies for A.fasciatus to acclimatize to lotic water where environmental factors were unstable but predictable. | Yunzhi YAN Lili GUO Xiuying XIANG Yilong XI Yifeng CHEN | 2009 | Current Zoology2009，55，5： | 9 |
| 13 | Protective effects of Jiayan Kangtai granules on autoimmune thyroiditis in a rat model by modulating Th17/Treg cell balance显示文摘OBJECTIVE: To investigate the protective effects of Jiayan Kangtai(JYKT) granules, consisting of 9 Chinese herbs, in a rat model of autoimmune thyroiditis(AIT), and the possible underlying mechanism.METHODS: Female Lewis rats(6-8 weeks) were randomly apportioned to 5 groups of 10, including a normal control. AIT was induced in the untreated AIT-model group, and rats treated subsequently with daily low, medium, or high dose JYKT granules. After 12 weeks, plasma levels of thyroid autoantibodies and morphological changes in the thyroid were detected by enzyme-linked immunosorbent assay and histological examination, respectively. The presence of interleukin(IL)-6,IL23 p19, and IL-2 in thyroid tissue was assessed by immunohistochemical staining. The percentages of T helper(Th)17 cells and regulatory T cells(Tregs) in the peripheral blood were analyzed by flow cytometry. Relevant levels of cytokines and proteins were examined via bead-based multiplex flow cytometry and ELISA, respectively. Expressions of genes and proteins regulated by Th17 cells and Tregs were shown by real-time PCR and Western blot.RESULTS: Compared to the control, AIT-model rats had higher plasma concentrations of thyroid autoantibodies. The high-dose JYKT rats showed significantly lower levels of thyroid autoantibodies compared with the AIT model group. Rats in the AIT-JYKT groups also had fewer thyroid lesions and less lymphocytic infiltration, a lower percentage of Th17 cells, and a higher percentage of Tregs, compared with the AIT-model. Rats given high-dose JYKT had a significantly lower Th17/Treg ratio compared with the AIT model. Differences in plasma cytokine concentrations and relevant gene and protein expressions in the spleens of JYKT-treated rats and the AIT group suggested an association between JYKT treatment and lower Th17 cell percentage and higher Treg activity.CONCLUSION: JYKT treatment appeared to be protective against AIT in rats, possibly via the regulation of the Th17 cell/Treg imbalance in AIT. | Hou Yi Wang Tieshan Guo Xiangyu Sun Wen Guo Xuan Wu Lili Qin Lingling Zhang Chengfei Liu Tonghua | 2018 | Journal of Traditional Chinese Medicine2018，38，3： | 9 |
| 14 | Integrated analysis of transcriptomic and proteomic data from tree peony(P.ostii)seeds reveals key developmental stages and candidate genes related to oil biosynthesis and fatty acid metabolism显示文摘Tree peony(Paeonia section Moutan DC.)seeds are an excellent source of beneficial natural compounds that promote health,and they contain high levels of alpha-linolenic acid(ALA).In recent years,tree peony has been emerging as an oil crop.Therefore,combined analysis of the transcriptome and proteome of tree peony(P.ostii)seeds at 25,32,39,53,67,81,88,95,and 109 days after pollination(DAP)was conducted to better understand the transcriptional and translational regulation of seed development and oil biosynthesis.A total of 38,482 unigenes and 2841 proteins were identified.A total of 26,912 differentially expressed genes(DEGs)and 592 differentially expressed proteins(DEPs)were clustered into three groups corresponding to the rapid growth,seed inclusion enrichment and conversion,and late dehydration and mature stages of seed development.Fifteen lipid metabolism pathways were identified at both the transcriptome and proteome levels.Pathway enrichment analysis revealed that a period of rapid fatty acid biosynthesis occurred at 53–88 DAP.Furthermore,211 genes and 35 proteins associated with the fatty acid metabolism pathway,63 genes and 11 proteins associated with the biosynthesis of unsaturated fatty acids(UFAs),and 115 genes and 24 proteins associated with ALA metabolism were identified.Phylogenetic analysis revealed that 16 putative fatty acid desaturase(FAD)-encoding genes clustered into four FAD groups,eight of which exhibited the highest expression at 53 DAP,suggesting that they play an important role in ALA accumulation.RT-qPCR analysis indicated that the temporal expression patterns of oil biosynthesis genes were largely similar to the RNA-seq results.The expression patterns of fatty acid metabolism-and seed development-related proteins determined by MRM were also highly consistent with the results obtained in the proteomic analysis.Correlation analysis indicated significant differences in the number and abundance of DEGs and DEPs but a high level of consistency in expression patterns and metabolic pathways.The results of the present study represent the first combined transcriptomic and proteomic analysis of tree peony seeds and provide insight into tree peony seed development and oil accumulation. | Xiaojing Wang Haiying Liang Dalong Guo Lili Guo Xiangguang Duan Qishi Jia Xiaogai Hou | 2019 | Horticulture Research2019，6，1： | 9 |
| 15 | LHPP inhibits cell growth and migration and triggers autophagy in papillary thyroid cancer by regulating the AKT/AMPK/mTOR signaling pathway显示文摘In recent decades,the incidence rate of papillary thyroid carcinoma(PTC)has been rapidly increasing.However,the molecular mechanism of the physiological and pathological processes of PTC is still largely unknown.Phospholysine phosphohistidine inorganic pyrophosphate phosphatase(LHPP)is a tum or suppressor and exerts anti-tumor effect in several human cancers,while the role and underlying mechanism of LHPP in PTC remain vague.In this study,we firstly evaluated the roles of LHPP in PTC and explored its underlying mechanism.Using clinical tissue samples,we detected the level of LHPP in PTC tissues and in matched adjacent normal tissues.Lower level of LHPP was found in PTC tissues than in matched adjacent normal tissues.Similar LHPP expression pattern was found in PTC cell lines when compared with that in normal human thyroid follicular epithelial cells.Then,we over-expressed LHPP in three PTC cell lines and results showed that ectopic LHPP expression consistently reduced cell viability,proliferation,and migration and triggered cell autophagy.Furthermore,over-expression of LHPP inhibited the activation of the AKT/mTOR pathway and promoted the AMPK signaling pathway.In addition,the activation of AKT/mTOR and inhibition of AMPK signaling pathways restored the role of ectopic LHPP expression in PTC cell lines,indicating that LHPP exerts its anti-tumor activity through regulating the AKT/AMPK/mTOR pathway.Ultimately,we illustrated that ectopic LHPP expression inhibited PTC tumor growth in vivo.In conclusion,we revealed that LHPP has an anti-tumor effect in PTC and indicated that LHPP might serve as an effective diagnostic and therapeutic target for PTC. | Wenyu Sun Kai Qian Kai Guo Lili Chen Jun Xiang Duanshu Li Yi Wu Qinghai Ji Tuanqi Sun Zhuoying Wang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，4： | 8 |
| 16 | Linear FM Signal Parameter Estimation Using STFT and FRFT显示文摘 | SHEN Liran YIN Qingbo LU Mingyu ZHANG Qi GUO Lili SHEN Tianen ZHAO Guoku NING Shushi | 2013 | Chinese Journal of Electronics2013，22，2： | 8 |
| 17 | Preparation of cationic polyacrylamide microsphere emulsion and its performance for permeability reduction显示文摘In this paper, cationic polyacrylamide microspheres(CPAM) were synthesized using acrylamide(AM) and methacryloyloxyethyl trimethyl ammonium chloride(TMAEMC) as monomers, ammonium sulfate as dispersant, poly(acryloyloxyethyl trimethyl ammonium chloride)(PAETAC) as dispersion stabilizer, and ammonium persulfate as initiator. The synthetic method was dispersion polymerization. The effects of monomer ratio(AM/TMAEMC), dispersant concentration, and dispersion stabilizer dosage on dispersion polymerization were systematically studied to determine the optimal preparation conditions. The structure and viscosity of the synthesized polymer were characterized by FTIR and capillary viscometry, respectively, and the particle sizes and distribution of the polymer microspheres were characterized by microscopy and dynamic light scattering, respectively. Finally, flow tests were conducted to measure the permeability reduction performance of the microspheres at various concentrations in sand packs with different permeability. Results show that CPAM emulsion of a solids content of 1wt% has excellent performance in low-to-medium permeability formations(< 1,000 mD), and the efficiency may reach above 90%. | Guo Aijun Geng Yiran Zhao Lili Li Jun Liu Dong Li Peng | 2014 | Petroleum Science2014，11，3： | 8 |
| 18 | Prediction of H7N9 epidemic in China显示文摘 | Zhang Zhaojie Xia Yao Lu Yi Yang Jingchao Zhang Luwen Su Hui Lin Lili Wang Guoling Wang Tongmei Lin Shao Guo Zhongmin Lu Jiahai | 2014 | Chinese Medical Journal2014，，2： | 8 |
| 19 | Bph30confers resistance to brown planthopperby fortifying sclerenchyma in rice leaf sheaths显示文摘Phloem-feeding insects cause massive losses in agriculture and horticulture.Host plant resistance to phloem-feeding insects is often mediated by changes in phloem composition,which deter insect settling and feeding and decrease viability.Here,we report that rice plant resistance to the phloem-feeding brown planthopper(BPH)is associated with fortification of the sclerenchyma tissue,which is located just beneath the epidermis and a cell layer or two away from the vascular bundle in the rice leaf sheath.We found that BPHs prefer to feed on the smooth and soft region on the surface of rice leaf sheaths called the long-cell block.We identified Bph30 as a rice BPH resistance gene that prevents BPH stylets from reaching the phloem due to the fortified sclerenchyma.Bph30 is strongly expressed in sclerenchyma cells and enhances cellulose and hemicellulose synthesis,making the cell walls stiffer and sclerenchyma thicker.The structurally fortified sclerenchyma is a formidable barrier preventing BPH stylets from penetrating the leaf sheath tissues and arriving at the phloem to feed.Bph30 belongs to a novel gene family,encoding a protein with two leucine-rich domains.Another member of the family,Bph40,also conferred resistance to BPH.Collectively,the fortified sclerenchyma-mediated resistance mechanism revealed in this study expands our understanding of plant-insect interactions and opens a new path for controlling planthoppers in rice. | Shaojie Shi Huiying Wang Lingyun Nie Di Tan Cong Zhou Qian Zhang YiLi Bo Du Jianping Guo Jin Huang DiWu Xiaohong Zheng Wei Guan Junhan Shan Lili Zhu Rongzhi Chen Longjian Xue Linda L.Walling Guangcun He | 2021 | Molecular Plant2021，14，10： | 7 |
| 20 | The effects of Haversian fluid pressure and harmonic axial loading on the poroelastic behaviors of a single osteon显示文摘In order to well understand the mechanism of the mechanotransduction in bone,we propose a new model of transverse isotropic and poroelastic osteon cylinder considering Haversian fluid pressure.The analytical pore pressure and velocity solutions are obtained to examine the fluid transport behavior and pressure distribution in a loaded osteon on two different exterior surface cases.Case I is stress free and fully permeable and case II is impermeable.The following are the results obtained.(i) The Haversian fluid may not be ignored because it can enlarge the whole osteonal fluid pressure field,and it bears the external loads together with the solid skeleton.(ii) The increase of both axial strain amplitude and frequency can result in the increase of fluid pressure and velocity amplitudes,while in case II,the frequency has little effect on the fluid pressure amplitude.(iii) Under the same loading conditions,the pressure amplitude in case II is larger than that in case I,while the velocity amplitude is smaller than that in case I.This model permits the linking of the external loads to the osteonal fluid pressure and velocity,which may be a stimulus to the mechanotransduction of bone remodeling signals. | WU XiaoGang CHEN WeiYi GAO ZhiPeng GUO HongMei WANG LiLi | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，9： | 7 |
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