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| 1 | Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping显示文摘Two microsatellite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial insemination. Eleven alleles were acquired at two microsatellite DNA loci (locus RS0622 and locus EN0033) by investigating 145 offsprings and 11 parents. Five alleles were acquired from locus RS0622 and six from locus EN0033. As analyzed, the gene frequencies were between 0.024 1 and 0.493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content (PIC) was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively. Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred. The pedigrees of three F1 and three F2 generations were determined by matching the genotype at each locus. | DONG Shirui KONG Jie ZHANG Qingwem LIU Ping MENG Xianhong WANG Rucai | 2006 | Acta Oceanologica Sinica2006，25，5： | 5 |
| 2 | Simulated effect of soil freeze-thaw process on surface hydrologic and thermal fluxes in frozen ground region of the Northern Hemisphere显示文摘Soil freeze-thaw process is closely related to surface energy budget,hydrological activity,and terrestrial ecosystems.In this study,two numerical experiments(including and excluding soil freeze-thaw process)were designed to examine the effect of soil freeze-thaw process on surface hydrologic and thermal fluxes in frozen ground region in the Northern Hemisphere based on the state-of-the-art Community Earth System Model version 1.0.5.Results show that in response to soil freeze-thaw process,the area averaged soil temperature in the shallow layer(0.0175−0.0451 m)decreases by 0.35℃in the TP(Tibetan Plateau),0.69℃in CES(Central and Eastern Siberia),and 0.6℃in NA(North America)during summer,and increases by 1.93℃in the TP,2.28℃in CES and 1.61℃in NA during winter,respectively.Meanwhile,in response to soil freeze-thaw process,the area averaged soil liquid water content increases in summer and decrease in winter.For surface heat flux components,the ground heat flux is most significantly affected by the freeze-thaw process in both summer and winter,followed by sensible heat flux and latent heat flux in summer.In the TP area,the ground heat flux increases by 2.82 W/m2(28.5%)in summer and decreases by 3.63 W/m2(40%)in winter.Meanwhile,in CES,the ground heat flux increases by 1.89 W/m2(11.3%)in summer and decreases by 1.41 W/m2(18.6%)in winter.The heat fluxes in the Tibetan Plateau are more susceptible to the freeze-thaw process compared with the high-latitude frozen soil regions.Soil freeze-thaw process can induce significant warming in the Tibetan Plateau in winter.Also,this process induces significant cooling in high-latitude regions in summer.The frozen ground can prevent soil liquid water from infiltrating to deep soil layers at the beginning of thawing;however,as the frozen ground thaws continuously,the infiltration of the liquid water increases and the deep soil can store water like a sponge,accompanied by decreasing surface runoff.The influence of the soil freeze-thaw process on surface hydrologic and thermal fluxes varies seasonally and spatially. | Di Ma SiQiong Luo DongLin Guo ShiHua Lyu XianHong Meng BoLi Chen LiHui Luo | 2021 | Research in Cold and Arid Regions2021，13，1： | 4 |
| 3 | Heterosis and heritability estimates for the survival of the Pacific white shrimp(Litopenaeus vannamei) under the commercial scale ponds显示文摘The aim of the present study is to detect the potential of the base population from diallel crosses of eight introduced strains of the Pacific white shrimp(Litopenaeus vannamei) for improving the yield. Heterosis and heritability were estimated for pond survival at commercial farm conditions for the base population that included 207 full-sib families from a nested mating design by artificial insemination. Among all the hybrids,the heterosis ranged from –11.37%(UA1×UA2) to 20.53%(UA3×SIN) with an average of 0.953%. The results showed that more than half of the hybrids(51.85%) have negative heterosis for survival rate, but most of the hybrids with positive heterosis have high estimates. The high proportion of negative heterosis for survival rate reminders us that the survival trait also should be considered in the crossbreeding program to avoid yield decrease. However, high positive heterosis manifested in most of the hybrids for survival indicates the usefulness of these hybrids for improving the survival to obtain higher yield by crossbreeding in this breeding program. The heritability estimate for pond survival was 0.092±0.043 when genetic groups were included in the pedigree, and it was significantly different from zero(P<0.05). The results from this study also indicated that significant improvement for survival is possible through selection in L. vannamei. | LU Xia LUAN Sheng CAO Baoxiang SUI Juan DAI Ping MENG Xianhong LUO Kun KONG Jie | 2017 | Acta Oceanologica Sinica2017，36，2： | 4 |
| 4 | Establishment of microsatellite-based triplex PCR for parentage analysis of Chinese shrimp Fenneropenaeus chinensis显示文摘Through exploring the microsatellite primers from the random genome sequences of Chinese shrimp (Fenneropenaeus chinensis), some microsatellite primers were obtained with rich polymorphic genetic information, and a triplex PCR was established using three primers (RS1101, RS0683 and H081 primers). By adjusting the final concentration of Mg2+, dNTP and primers, and using a touch-town PCR program, the optimum amplification parameters of PCR system were obtained, which could successfully amplify the three primers in a PCR reaction. In the denatured PAGE gel, the amplified DNA fragments of three primers RS1101, RS0683 and H081 could be easily identified each other. For the triplex PCR system, the PPE (probabilities of paternity exclusion) is 0.967 9, and the DP (discrimination power) is 0.999 327. Using the triplex PCR to test ten individuals of a parentage and their parents, an individual was excluded from the parentage in all of the three microsatellite loci, which might be mixed into the parentage for some unknown reason such as factitious misplay. The triplex PCR will be of great practical value in identifying the parentages of F. chinensis. | GAO Huan KONG Jie HU Ping MENG Xianhong LUAN Sheng ZHANG Tianshi | 2007 | Acta Oceanologica Sinica2007，26，2： | 4 |
| 5 | An improvement of soil temperature simulations on the Tibetan Plateau显示文摘The simulation of soil temperature on the Tibetan Plateau(TP) plays a dominant role in the performance of both global climate and numerical weather forecast models. To improve the simulation of soil temperature on the TP, the Johansen soil thermal conductivity parameterization scheme was introduced into Community Land Model 3.5(CLM3.5) and Regional Climatic Model 4(Reg CM4). The improved CLM3.5 and Reg CM4-CLM were utilized to conduct offline and regional simulation experiments on the TP. Comparison of the new and old schemes revealed that CLM3.5 provides high thermal conductivity parameters of mineral soil solid on the TP. The Johansen scheme is more practical for the TP than the soil thermal conductivity parameterization in CLM3.5. The simulation of soil temperature and liquid water content was improved in offline experiment. The improved parameterization scheme can also reduce the simulation error of soil temperature in winter throughout the entire TP. | SiQiong Luo BoLi Chen ShiHua Lyu XueWei Fang JingYuan Wang XianHong Meng LunYu Shang ShaoYing Wang Di Ma | 2018 | Research in Cold and Arid Regions2018，10，1： | 2 |
| 6 | A PIECEWISE LINEAR MODEL FOR ANALYZING THIN FILM/SUBSTRATE STRUCTURE IN FLEXIBLE ELECTRONICS显示文摘The conventional analytical method of predicting strain in a thin film under bending is restricted to the uniform material assumption, while in flexible electronics, the film/substrate structure is widely used with mismatched material properties taken into account. In this paper,a piecewise model is proposed to analyze the axial strain in a thin film of flexible electronics with the shear modification factor and principle of virtual work. The excellent agreement between analytical prediction and finite element results indicates that the model is capable of predicting the strain of the film/substrate structure in flexible electronics, whose mechanical stability and electrical performance is dependent on the strain state in the thin film. | Xianhong Meng Ming Li Yilin Xing Haijun Wang | 2014 | Acta Mechanica Solida Sinica2014，27，4： | 2 |
| 7 | Inconsistencies of precipitation in the eastern and central Tibetan Plateau between surface adjusted data and reanalysis显示文摘 | Qinglong You Klaus Fraedrich Guoyu Ren Baisheng Ye Xianhong Meng Shichang Kang | 2012 | Theoretical and Applied Climatology2012，，3： | 1 |
| 8 | Transport of Schistosoma japonicum cercariae and the feasibility of niclosamide for cercariae control显示文摘 | Debbie Lowe Jinyu Xi Xianhong Meng etal | 2005 | Parasitology International2005，54，1： | 1 |
| 9 | The evapotranspiration and environmental controls of typical underlying surfaces on the Qinghai-Tibetan Plateau显示文摘To reveal the characteristics of evapotranspiration and environmental control factors of typical underlying surfaces(alpine wetland and alpine meadow)on the Qinghai-Tibetan Plateau,a comprehensive study was performed via in situ observations and remote sensing data in the growing season and non-growing season.Evapotranspiration was positively correlated with precipitation,the decoupling coefficient,and the enhanced vegetation index,but was energy-limited and mainly controlled by the vapor pressure deficit and solar radiation at an annual scale and growing season scale,respectively.Compared with the non-growing season,monthly evapotranspiration,equilibrium evaporation,and decoupling coefficient were greater in the growing season due to lower vegetation resistance and considerable precipitation.However,these factors were restricted in the alpine meadow.The decoupling factor was more sensitive to changes of conductance in the alpine wetland.This study is of great significance for understanding hydro-meteorological processes on the Qinghai-Tibetan Plateau. | JinLei Chen Jun Wen ShiChang Kang XianHong Meng XianYu Yang | 2021 | Research in Cold and Arid Regions2021，13，1： | 1 |
| 10 | An evaluation of soil moisture from AMSR-E over source area of the Yellow River, China显示文摘In this study,in-situ soil moisture measurements are used to evaluate the accuracy of three AMSR-E soil moisture prod ucts from NASA(National Aeronautics and Space Administration),JAXA(Japanese Aerospace Exploration Agency)and VUA(Vrije University Amsterdam and NASA)over Maqu County,Source Area of the Yellow River(SAYR),China.Re sults show that the VUA soil moisture product performs the best among the three AMSR-E soil moisture products in the study area,with a minimum RMSE(root mean square error)of 0.08(0.10)m3/m3 and smallest absolute error of 0.07(0.08)m3/m3 at the grassland area with ascending(descending)data.Therefore,the VUA soil moisture product is used to describe the spatial variation of soil moisture during the 2010 growing season over SAYR.The VUA soil moisture product shows that soil moisture presents a declining trend from east south(0.42 m3/m3)to west north(0.23 m3/m3),with good agreement with a general precipitation distribution.The center of SAYR presents extreme wetness(0.60 m3/m3)dur ing the whole study period,especially in July,while the head of SAYR presents a high level soil moisture(0.23 m3/m3)in July,August and September. | TangTang Zhang Mekonnen Gebremichael Akash Koppa XianHong Meng Qun Du Jun Wen | 2019 | Research in Cold and Arid Regions2019，11，6： | 1 |
| 11 | Screening White Spot Syndrome Virus(WSSV)-Resistant Molecular Markers from Fenneropenaeus chinensis显示文摘White spot syndrome virus(WSSV)-resistant molecular markers were screened from the selectively bred new variety ‘Huanghai No. 2' of Fenneropenaeus chinensis using unlabeled-probe high-resolution melting(HRM) technique. After the artificial infection with WSSV, the first 96 dead shrimps and the last 96 surviving shrimps were collected, representing WSSV-susceptible and-resistant populations, respectively. The genotypes at well-developed 39 single nucleotide polymorphisms(SNPs) loci were obtained. As revealed in the Chi-square test, 3 SNPs, genotype A/A of contig C364-89 AT, genotype A/A of C2635-527 CA and genotype C/T of contig C12355-592 CT, were positively correlated with disease-resistance traits. Other 2 SNPs, genotype G/G of contig C283-145 AG and genotype C/C of contig C12355-592 CT, were negatively correlated. Moreover, analysis with Blast X program for disease-resistant SNPs indicated that 3 contigs, Contig283, Contig364 and Contig12355, matched to the functional genes of effector caspase of Penaeus monodon, peptide transporter family 1-like protein, and 40 S ribosomal protein S2 of Perca flavescens with high sequence similarity. The results will be helpful to provide theoretical and technical supports for molecular marker-assisted selective breeding of F. chinensis. | WU Yingying MENG Xianhong KONG Jie LUAN Sheng LUO Kun WANG Qingyin ZHENG Yongyun | 2017 | Journal of Ocean University of China2017，16，1： | 1 |
| 12 | Genetic Parameter Estimation of Reproductive Traits of Litopenaeus vannamei显示文摘In this study, the heritability, repeatability, phenotypic correlation, and genetic correlation of the reproductive and growth traits of L. vannamei were investigated and estimated. Eight traits of 385 shrimps from forty-two families, including the number of eggs(EN), number of nauplii(NN), egg diameter(ED), spawning frequency(SF), spawning success(SS), female body weight(BW) and body length(BL) at insemination, and condition factor(K), were measured,. A total of 519 spawning records including multiple spawning and 91 no spawning records were collected. The genetic parameters were estimated using an animal model, a multinomial logit model(for SF), and a sire-dam and probit model(for SS). Because there were repeated records, permanent environmental effects were included in the models. The heritability estimates for BW, BL, EN, NN, ED, SF, SS, and K were 0.49 ± 0.14, 0.51 ± 0.14, 0.12 ± 0.08, 0, 0.01 ± 0.04, 0.06 ± 0.06, 0.18 ± 0.07, and 0.10 ± 0.06, respectively. The genetic correlation was 0.99 ± 0.01 between BW and BL, 0.90 ± 0.19 between BW and EN, 0.22 ± 0.97 between BW and ED,-0.77 ± 1.14 between EN and ED, and-0.27 ± 0.36 between BW and K. The heritability of EN estimated without a covariate was 0.12 ± 0.08, and the genetic correlation was 0.90 ± 0.19 between BW and EN, indicating that improving BW may be used in selection programs to genetically improve the reproductive output of L. vannamei during the breeding. For EN, the data were also analyzed using body weight as a covariate(EN-2). The heritability of EN-2 was 0.03 ± 0.05, indicating that it is difficult to improve the reproductive output by genetic improvement. Furthermore, excessive pursuit of this selection is often at the expense of growth speed. Therefore, the selection of high-performance spawners using BW and SS may be an important strategy to improve nauplii production. | TAN Jian KONG Jie CAO Baoxiang LUO Kun LIU Ning MENG Xianhong XU Shengyu GUO Zhaojia CHEN Guoliang LUAN Sheng | 2017 | Journal of Ocean University of China2017，16，1： | 1 |
| 13 | Numerical simulations of the atmospheric and land conditions over the Jinta Oasis in Northwestern China with satellite-derived land surface parameters显示文摘 | Meng Xianhong L Shihua Zhang Tangtang | 2009 | J Geophys Res2009，114，： | 1 |
| 14 | Influence of White Spot Syndrome Virus Infection on Hepatopancreas Gene Expression of ‘Huanghai No.2' Shrimp(Fenneropenaeus chinensis)显示文摘To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus(WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of ‘Huanghai No. 2'(Fenneropenaeus chinensis). A total of 59137 unigenes were designed onto a custom-made 60 K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early(48–96 h), peak(168–192 h) and late(264–288 h) infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes(1916 annotated) expressed at all three phases, and most of the annotated were either up-or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including β-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp's response to WSSV. | MENG Xianhong SHI Xiaoli KONG Jie LUAN Sheng LUO Kun CAO Baoxiang LIU Ning LU Xia LI Xupeng DENG Kangyu CAO Jiawang ZHANG Yingxue ZHANG Hengheng | 2017 | Journal of Ocean University of China2017，16，5： | 1 |
| 15 | Using Composite Phenotypes to Reveal Hidden Physiological Heterogeneity in High-Altitude Acclimatization in a Chinese Han Longitudinal Cohort显示文摘Altitude acclimatization is a human physiological process of adjusting to the decreased oxygen availability.Since several physiological processes are involved and their correlations are complicated,the analyses of single traits are insufficient in revealing the complex mechanism of high-altitude acclimatization.In this study,we examined these physiological responses as the composite phenotypes that are represented by a linear combination of physiological traits.We developed a strategy that combines both spectral clustering and partial least squares path modeling(PLSPM)to define composite phenotypes based on a cohort study of 883 Chinese Han males.In addition,we captured 14 composite phenotypes from 28 physiological traits of high-altitude acclimatization.Using these composite phenotypes,we applied k-means clustering to reveal hidden population physiological heterogeneity in high-altitude acclimatization.Furthermore,we employed multivariate linear regression to systematically model(Models 1 and 2)oxygen saturation(SpO_(2))changes in high-altitude acclimatization and evaluated model fitness performance.Composite phenotypes based on Model 2 fit better than single trait-based Model 1 in all measurement indices.This new strategy of using composite phenotypes may be potentially employed as a general strategy for complex traits research such as genetic loci discovery and analyses of phenomics. | Yi Li Yanyun Ma Kun Wang Menghan Zhang Yi Wang Xiaoyu Liu Meng Hao Xianhong Yin Meng Liang Hui Zhang Xiaofeng Wang Xingdong Chen Yao Zhang Wenyuan Duan Longli Kang Bin Qiao Jiucun Wang Li Jin | 2021 | Phenomics2021，1，1： | 1 |
| 16 | Effect of Poly-β-hydroxybutyrate on Reproductive Performance and Larval Development of Fenneropenaeus chinensis显示文摘In the present study, Fenneropenaeus chinensis individuals were assigned into six groups, and fed with a diet supplemented with 0(Con-trol), 0.5%(Group E0.5), 1.0%(Group E1.0), 2.0%(Group E2.0), 3.0%(Group E3.0) or 5.0%(Group E5.0) of poly-β-hydroxybutyrate(PHB) during their growth and spawning periods, to explore the effect of PHB on the reproductive performance of parents and the development of larvae. The experi-ment was lasted for 192 d to measure the mortality rate, relative percentage of survival(RPS), weight gain rate(WGR), specific growth rate(SGR) and cycle of gonadal development of parental shrimps, number of eggs, number of nauplii, stages of larval development and metamorphosis. The results indicated that PHB had an obvious effect on the fecundity of parental shrimps and growth performance of larva. Compared with control group, the mortality rate of broodstock in experimental groups decreased initially and increased subsequently with the increase of PHB concentration, while RPS showed an opposite pattern. The mortality rate of group E2.0 was the lowest among all groups, showing significant difference from that of other groups(P﹤0.05) except group E1.0(P﹥0.05). WGR of all experimental groups was higher than that of the control group, among which, group E2.0 and group E5.0 had significantly higher WGR than the control(P<0.05). However, there was no significant difference in the cycle of gonadal development between the experimental groups and the control group(P ﹥ 0.05). The numbers of eggs and nauplii in the experimental groups were higher than those of the control group. And the numbers of eggs and nauplii in group E2.0 were significantly higher than those of the control(P ﹤ 0.05), bud showed no significant difference from those of other groups(P﹥0.05). However, there was no significant difference in the duration of larval devel-opment and metamorphosis among all the groups. In summary, PHB is capable of improving the reproductive performance of parents and the survival and development of larva in F. chinensis, and 2.0% is the optimal concentration of PHB supplemented to the diet. | Zhang Hengheng Meng Xianhong Kong Jie Luo Kun Luan Sheng Cao Baoxiang Cao Jiawang Zhang Yingxue | 2018 | Animal Husbandry and Feed Science2018，10，3： | 0 |
| 17 | Comparison of Genetic Diversity and Growth Performance between the Selected Population and Hybridized Population of Litopenaeus vannamei at Low Temperature显示文摘[Objective] This study was conducted to compare the genetic diversity and growth performance of two Litopenaeus vannamei populations at low temperature. [Method] One population(selected population, SP) was produced through inbreeding and 3 years of selection from seven popula-tions of L. vannamei introduced from the United States and Singapore. The other population(hybridized population, HP) was the F1 generation of the crosses between Population SP and some excellent populations introduced from South Korea and Singapore. The growth performance of the two populations at low temperature was compared, and the genetic diversity and genetic differentiation between the two populations were assessed using13 microsatellite loci. [Result] The shrimps of population HP had better growth performance than those of population SP. The average body weight(BW) for population HP, which was(13.18 ±3.65) g/ind., was significantly higher than that of population SP, which was(12.20 ±3.14) g/ind. The coefficient of variation in body weight for population SP was 25.74%, and that for population HP was 27.69%. The other growth indices of popula-tion HP were all higher than those of population SP. One-way analysis of variance(ANOVA) indicated that there were highly significant differences in both BW and width of third abdominal segment(WTAS) between HP and SP( P<0.001). The specific growth rate(SGR) and absolute growth rate(AGR) for population HP were(5.09±0.61) %/d and(0.26±0.60) g/d, respectively, while the SGR and AGR for population SP were(4.94 ±0.57) %/d and(0.24±0.63) g/d., both significantly lower than those of population HP(P<0.001). Genetic diversity analysis revealed that the number of alleles(Na) of population HP(Na=7.9) was slightly higher than that of population SP(Na=7.6). The average polymorphic information content(PIC) of HP and SP populations was similar 0.63 and 0.62, both indicating high level of genetic diversity. The average observed heterozygosity( Ho) of HP and SP was0.492 and 0.483, and the expected heterozygosity(He) was 0.675 and 0.663, respectively. Both Hoand Heof population HP were higher than those of population SP, suggesting that HP had higher genetic diversity than population SP. Moreover, the mean of Fs Tvalues at the 13 microsatellite loci between HP and SP was 0.155 6, suggesting there was a significant genetic differentiation between the two populations. [Conclusion] Our results provide a theoretical basis for the breeding of new L. vannamei strains that are resistant to low temperature. | Li Dongyu Meng Xianhong Kong Jie Luan Sheng Cao Baoxiang Luo Kun | 2018 | Animal Husbandry and Feed Science2018，10，3： | 0 |
| 18 | Global Land Surface Climate Analysis Based on the Calculation of a Modified Bowen Ratio显示文摘A modified Bowen ratio(BRm),the sign of which is determined by the direction of the surface sensible heat flux,was used to represent the major divisions in climate across the globe,and the usefulness of this approach was evaluated. Five reanalysis datasets and the results of an offline land surface model were investigated. We divided the global continents into five major BRm zones using the climatological means of the sensible and latent heat fluxes during the period 1980–2010:extremely cold,extremely wet,semi-wet,semi-arid and extremely arid. These zones had BRm ranges of(-∞,0),(0,0.5),(0.5,2),(2,10) and(10,+∞),respectively. The climatological mean distribution of the Bowen ratio zones corresponded well with the K ¨oppen-like climate classification,and it reflected well the seasonal variation for each subdivision of climate classification. The features of climate change over the mean climatological BRm zones were also investigated. In addition to giving a map-like classification of climate,the BRm also reflects temporal variations in different climatic zones based on land surface processes. An investigation of the coverage of the BRm zones showed that the extremely wet and extremely arid regions expanded,whereas a reduction in area was seen for the semi-wet and semi-arid regions in boreal spring during the period 1980–2010. This indicates that the arid regions may have become drier and the wet regions wetter over this period of time. | Bo HAN Shihua Lü Ruiqing LI Xin WANG Lin ZHAO Cailing ZHAO Danyun WANG Xianhong MENG | 2017 | Advances in Atmospheric Sciences2017，34，5： | 0 |
| 19 | Effect of Different Diet Combinations on WSSV Load and Survival Rate of Fenneropenaeus chinensis Larvae显示文摘Using microalgae( SA),artificial feed( AF),Brachionus plicatilis( BP) and brine shrimp larvae( BS),four diets including SA + AF,SA + AF + BP,SA + AF + BS and SA + AF + BP + BS were formulated and used to rear Fenneropenaeus chinensis larvae. The fertilized eggs fed with SA + AF were divided into disinfection group with iodophor and untreated group,and the fertilized eggs fed with other diets were all disinfected with iodophor. The survival rates,body weight and WSSV load of post larvae at each stage were compared. The results showed that there was no significant difference in the survival rate of prawns at the N-Z stage by feeding with different diets( P > 0. 05),but the survival rate of prawns fed with BP was higher than others( P < 0. 01) at the Z-P stage. The body weight of post larvae( P10) fed with SA + AF + BP was less than that fed with SA + AF + BP + BS( P <0. 01). The WSSV load of prawns( 10. 52 ±3. 3 copies/ng DNA) fed with SA + AF were significantly lower than that of other treatments( P < 0. 05) at the P10 stage. During the P11- P60 stage,four kinds of diets,including Ruditapes philippinarum foot muscle( CF) + artificial feed( AF),R. philippinarum foot muscle radiated by60 Co γ-ray( RCF) + artificial feed( AF),potassium permanganate-disinfected R. philippinarum foot muscle( DCF) + artificial feed( AF) and artificial feed( AF),were used to feed prawns. The prawns fed with CF + AF gained the greatest increase of body weight and body length,but had no significant difference with that fed with AF( P > 0. 05); prawns fed with CF + AF and AF had significant difference with the other two groups( P < 0. 05). The survival rates of prawns fed with four different diets had no significant difference with each other( P > 0. 05). WSSV artificial infection test showed that the accumulated mortality of prawns fed with four diets were higher than 90%,and the difference was not significant( P > 0. 05). | Xiao Guangxia Meng Xianhong Kong Jie Cao Baoxiang Liu Ning Zhang Qingwen Luo Kun Li Zhanjun | 2015 | Animal Husbandry and Feed Science2015，7，6： | 0 |
| 20 | Analytical study of wrinkling in thin-film-on-elastomer system with finite substrate thickness显示文摘Surface wrinkling, a film bonded on a pre-strained elastomeric substrate can form periodic wrinkling patterns, is a common phenomenon in daily life. In existing theoretical models, the film is much thinner than the substrate so that the substrate can be considered to be elastomeric with infinite thickness. In this paper, the effect of finite substrate thickness is analyzed theoretically for free boundary condition cases. Based on the minimum potential energy principle, a theoretical model is established, and the wave length and amplitude of the wrinkling pattern are obtained. When the thickness of the substrate is more than 200 times larger than the thickness of the film, the results of this study agree well with the results obtained from the previous models for infinite substrate thickness. However, for thin substrates, the effect of finite substrate thickness becomes significant. The model given in this paper accurately describes the effect of finite substrate thickness, providing important design guidelines for future thin-film-on-substrate systems such as stretchable electronic devices. | Xianhong MENG Guanyu LIU Zihao WANG Shuodao WANG | 2017 | Applied Mathematics and Mechanics(English Edition)2017，38，4： | 0 |
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