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| 1 | Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo显示文摘BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor metastasis.However,its role in hepatocellular carcinoma(HCC) cells remains unknown.METHODS:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical staining.Apoptosis was evaluated by TUNEL assay.Cell viability and apoptosis were determined by MTT assay and flow cytometry,respectively.Apoptosis-related proteins in SMMC-7721 and HepG2 cells were detected by Western blotting.RESULTS:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of 12-LOX.Baicalein inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent manner.Our in vivo study demonstrated that baicalein also reduced neoplastic nodules.Mechanistically,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of caspase-3.Furthermore,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of 12-LOX.CONCLUSIONS:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment. | Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，2： | 20 |
| 2 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 3 | Interferon-c and interleukin-4 detected in serum and saliva from patients with oral lichen planus显示文摘Our previous salivary study had demonstrated an apparent T helper 2(Th2)-predominance in saliva of oral lichen planus(OLP) patients and suggested a potential of salivary interleukin-4(IL-4) as a biomarker for monitoring disease severity. To further determine the consistency of Th1/Th2 bias of OLP, this study investigated the expression profile of interferon-c(IFN-c) and IL-4 in serum and the relationship of the serum levels of these cytokines with their saliva partners. Sixty ethnic Chinese patients with OLP(40 of the erythematous/ulcerative form and 20 of the reticular form) were recruited for this study, with 40 age–sex-matched healthy volunteers as control group. IFN-c and IL-4 levels in serum and paired saliva samples were screened by enzyme-linked immunosorbent assay. OLP patient showed a low-level IFN-c but high-level IL-4 expression profile in both serum and saliva, with a lower IFN-c/IL-4 ratio. Serum IL-4 level in the erythematous/ulcerative group was significantly higher than that in the reticular group. Serum levels of IFN-c and IL-4 were significantly and positively correlated with their saliva partners. These results provided more evidence for Th2 cytokinepredominant immune imbalance in OLP, as well as the potential of IL-4 as the biomarker for monitoring severity of OLP. | Wen-Zhao Liu Ming-Jing He Long Long Dong-Liang Mu Ming-Shu Xu Xue Xing Xin Zeng Ga Liao Hong-Xia Dan Qian-Ming Chen | 2014 | International Journal of Oral Science2014，6，1： | 10 |
| 4 | The Oral Microbiome Bank of China显示文摘The human microbiome project(HMP) promoted further understanding of human oral microbes. However, research on the human oral microbiota has not made as much progress as research on the gut microbiota. Currently, the causal relationship between the oral microbiota and oral diseases remains unclear, and little is known about the link between the oral microbiota and human systemic diseases. To further understand the contribution of the oral microbiota in oral diseases and systemic diseases, a Human Oral Microbiome Database(HOMD) was established in the US. The HOMD includes 619 taxa in 13 phyla, and most of the microorganisms are from American populations. Due to individual differences in the microbiome, the HOMD does not reflect the Chinese oral microbial status. Herein, we established a new oral microbiome database—the Oral Microbiome Bank of China(OMBC,http://gffzz1f92d53390b34408hpqoqfv5v0qqb6ocn.ffgz.tsg.suse.edu.cn/ombc). Currently, the OMBC includes information on 289 bacterial strains and 720 clinical samples from the Chinese population, along with lab and clinical information. The OMBC is the first curated description of a Chinese-associated microbiome; it provides tools for use in investigating the role of the oral microbiome in health and diseases, and will give the community abundant data and strain information for future oral microbial studies. | Xian Peng Xuedong Zhou Xin Xu Yuqing Li Yan Li Jiyao Li Xiaoquan Su Shi Huang Jian Xu Ga Liao | 2018 | International Journal of Oral Science2018，10，2： | 8 |
| 5 | Effects of salinity on methane gas hydrate system显示文摘Using an approximately analytical formation, we extend the steady state model of the pure methane hydrate system to include the salinity based on the dynamic model of the methane hydrate system. The top and bottom boundaries of the methane hydrate stability zone (MHSZ) and the actual methane hy-drate zone (MHZ), and the top of free gas occurrence are determined by using numerical methods and the new steady state model developed in this paper. Numerical results show that the MHZ thickness becomes thinner with increasing the salinity, and the stability is lowered and the base of the MHSZ is shifted toward the seafloor in the presence of salts. As a result, the thickness of actual hydrate occur-rence becomes thinner compared with that of the pure water case. On the other hand, since lower solubility reduces the amount of gas needed to form methane hydrate, the existence of salts in sea-water can actually promote methane gas hydrate formation in the hydrate stability zone. Numerical modeling also demonstrates that for the salt-water case the presence of methane within the field of methane hydrate stability is not sufficient to ensure the occurrence of gas hydrate, which can only form when the methane concentration dissolved in solution with salts exceeds the local methane solubility in salt water and if the methane flux exceeds a critical value corresponding to the rate of diffusive methane transport. In order to maintain gas hydrate or to form methane gas hydrate in marine sedi-ments, a persistent supplied methane probably from biogenic or thermogenic processes, is required to overcome losses due to diffusion and advection. | YANG DingHui1 & XU WenYue2 1 Department of Mathematical Sciences, Tsinghua University, Beijing 100084, China 2 School of Earth & Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA | 2007 | Science China Earth Sciences2007，50，11： | 5 |
| 6 | Biofilm resistance to antimicrobial agents显示文摘 | Xu KD McFeters GA Stewart PS | 2000 | Microbiology2000，146，3： | 1 |
| 7 | Fuzzy Fault Diagnosis of a Diesel Engine Non-start显示文摘The diesel locomotive plays an important role in the field of transport,and the engine maintenance work is the prerequisite and guarantee for the locomotive normal working.In this paper,we first establish the fault tree model of locomotive engine 16V240ZJ on the basis of engine non-start as the top event.Then we combines the fuzzy mathematics theory and fault tree analysis method for failure diagnosis of 16V240ZJ engine's abnormal start-up.We obtained the fuzzy probability curve and top events probability confidence interval by analyzing the fuzzy fault tree qualitatively and quantitatively.It provides a fuzzy analysis basis for solving the problem of 16V240ZJ engine's abnormal start-up. | LIU Ke-ming YANG Wei-hong XU Guang-ming XU Wei-guo GA O Lei-fu | 2009 | International Journal of Plant Engineering and Management2009，14，3： | 1 |
| 8 | Outcome after portoenterostomy in biliary atresia:pivotal role of degree of liver fibrosis and intensity of stellate cell activation显示文摘 | Ramm GA Xu C | 2006 | J Pediatr Gastroenterol Nutr2006，42，： | 1 |
| 9 | Biofilm resistance to antimicrobial agents显示文摘 | Xu KD McFeters GA Stewart PS | 2000 | Microbiology2000，146，3： | 1 |
| 10 | Allergic asthma accelerates atherosclerosis dependent on Th2 and Thl7 in apolipoprotein E de- ficient mice 显示文摘 | Li jun Wang Shah shan Ga Wei Xu | 2014 | Journal of Molecular and Cellular Cardiology2014，，： | 1 |
| 11 | Effect of different whiskers on the physical and trihologieal properties of non-metallic friction mate- rials 显示文摘 | Zhu ZC Xu L Chen GA | 2011 | Materials and Design2011，32，1： | 1 |
| 12 | Post-initiation effects of chlo- rophyllin and indole-3-carbinol in rats given 1, 2-dimethyl- hydrazine or 2-amino-3-methyl-imidazo 4,5-f quinoline显示文摘 | Xu M Orner GA Bailey GS | 2001 | Carci- nogenesis2001，22，2： | 1 |
| 13 | Differential roles for disulfide bonds in the structural integrity and biological activity of к-bugatotoxin,a neuronal nicotinic acetylcholine receptor antagonist显示文摘 | Grara GA Luetje CW Summers R Xu XL | 1998 | Biochemistry1998，37，12： | 1 |
| 14 | Outcome after portoenterostomy in biliary atresia:Pivotal role of degree of liver fibrosis and intensity of stellate cell activation显示文摘 | Shteyer E Ramm GA Xu C | 2006 | J Pediatr Gastroenterol Nutr2006，42，1： | 1 |
| 15 | Postural sway in idiopathic rapid eye movement sleep behavior disorder:a potential marker of prodromal Par- kinson's disease 显示文摘 | Chen TZ Xu GJ Zhou GA | 2014 | Brain Res2014，1559，： | 1 |
| 16 | Enhanced expression of neuronal proteins in idiopathic frozen shoulder显示文摘 | Xu Y Bonar F Murrell GA | 2012 | J Shoulder Elbow Surg2012，21，10： | 1 |
| 17 | Enhanced expression of neuronal proteins inidiopathic frozen shoulder显示文摘 | Xu Y Bonar F Murrell GA | 2012 | J Shoulder Elbow Surg2012，21，10： | 1 |
| 18 | Controlled formation of volatile components in cider making Saccharomyces cerevisiae using a combination of and Hanseniaspora valbyensisyeast species 显示文摘 | XU Y ZHAO GA WANG LP | 2006 | J Ind Microbiol Blot2006，33，3： | 1 |
| 19 | The osmium-catalyzed asymmetric dihydroxylatiom: A new ligand class and a process improvement 显示文摘 | Amberg W Bennani YL Crispino GA Hartung J Jeong KS Kwong HL Morikawa K Wang ZM Xu DQ Zhang XL | 1992 | J Org Chem1992，57，10： | 1 |
| 20 | Organising a juvenile ratio monitoring programme for 10 key waterbird species in the Yangtze River floodplain:analysis and proposals显示文摘Background:In the face of continued degradation and loss of wetlands in the Yangtze River floodplain(YRF),there is an urgent need to monitor the abundance and distribution of wintering waterbirds.To understand fully observed annual changes,we need to monitor demographic rates to understand factors affecting global population size.Annual reproduction success contributes to dynamic changes in population size and age structure,so an assessment of the juvenile ratio(i.e.first winter birds as a proportion of total number aged)of overwintering waterbirds can be an important indicator of the reproductive success in the preceding breeding season.Methods:During 2016-2019,we sampled juvenile ratios among 10 key waterbird species from the wetlands in the YRF.Based on these data,we here attempt to establish a simple,efficient,focused and reliable juvenile ratio monitoring scheme,to assess consistently and accurately relative annual breeding success and its contribution to the age structure among these waterbird species.Results:We compared juvenile ratio data collected throughout the winter and found that the optimal time for undertaking these samples was in the early stages of arrival for migratory waterbirds reaching their wintering area(early to mid-December).We recommend counting consistently at key points(i.e.those where>1%biogeographical flyway population were counted)at sites of major flyway importance(Poyang Lake,East Dongting Lake,Shengjin Lake,Caizi Lake,Longgan Lake and Chen Lake).Based on this,the error rate of the programme(155 planned points,the count of 10 waterbird species is 826-8955)is less than 5%.Conclusions:We established a juvenile ratio monitoring programme for 10 key waterbird species in the wetlands of the YRF,and discuss the feasibility and necessity of implementing such a future programme,and how to use these data in our monitoring and understanding of the population dynamics of these waterbird populations. | Yuxi Wang Iderbat Damba Qingshan Zhao Yanbo Xie Xueqing Deng Rdi Ga Guanhua Liu Zhiwen Xu Yue Li Dali Gao Wenbin Xu Guoxun Chen Lei Cao | 2021 | Avian Research2021，12，4： | 1 |
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