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| 1 | Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing显示文摘Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks(DSBs)in vivo is a prerequisite for any genome editing efforts.Several engineered nucleases including zinc finger nucleases(ZFNs)and transcriptional activator-like nucleases(TALENs)have been successfully used to edit genomes.However, | Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 2 | Experimental investigations on separation control and flow structure around a circular cylinder with synthetic jet显示文摘Circular cylinder separation control and flow structure influenced by the synthetic jet have been experimentally investigated in a water channel. The synthetic jet is- sues from a slot and ejects toward upstream from the front stagnation point of the cylinder. It has been found that, similar to the traditional synthetic jet which is po- sitioned near the separation point or inside the separation region, the present synthetic jet arrangement constitutes an efficient way to control flow separation of the circular cylinder, but with a different control mechanism. The present synthetic jet leads to an upstream displacement of the front stagnation point and the forma- tion of a vortex pair near both sides of the exit orifice. When ReU based on the synthetic jet average exit orifice velocity is about lower than 43, a closed envelope forms in front of the windward side of the cylinder during the blowing cycle of syn- thetic jet, which acts as an apparent modification for the cylinder configuration. When ReU is high enough, an open envelope forms upstream of the cylinder, and the flow around the cylinder becomes much energetic. Thus, regardless of ReU, the present synthetic jet can improve separation for flow around a circular cylinder. With regard to the leeward side, as ReU increases, the flow separation region be- hind the cylinder gradually disappears. The flow over cylinder may be fully attached when the open envelope forms upstream of the cylinder and ReU is greater than 344. Then, the flow past the cylinder will converge near the back stagnation point of the cylinder, where a new vortex pair shedding periodically is generated due to the high shear layer. | WANG JinJun FENG LiHao XU ChaoJun | 2007 | Science China(Technological Sciences)2007，50，5： | 9 |
| 3 | CO removal by two-stage methanation for polymer electrolyte fuel cell显示文摘为了移开公司完成,降低公司内容在在为聚合物电解质的 reformer 的 CO 移动步的 10 ppm 下面燃料房间(PEFC ) 合作产生系统,在各种各样的条件下面的公司优先的 methanation 在这篇论文被学习。结果证明与催化剂的一种单个类型,到达两公司移动深度和公司 2 变换比率是困难的低于 5% 。因此,使用二种催化剂的一个二阶段的 methanation 过程在这研究被建议,也就是说有相对低的活动的一个种催化剂和为在更高的温度的第一个阶段的高选择,并且有为在更低的温度的第二个阶段的相对高的活动和高选择的另一种催化剂。试验性的结果证明在第一个阶段公司,内容从 1% 被减少到低于 0.1% 在 250 300 ° C,并且在第二个阶段到在在 150 185 ° C 的 10 ppm 下面。公司 2 变换被作为不到 5% 。同时,在公司移动深度上入口公司内容和 GHSV 影响也在这篇论文被讨论。 | Zhiyuan Li Wanliang Mi Juan Gong Zhenlong Lu Lihao Xu Qingquan Su | 2008 | Journal of Natural Gas Chemistry2008，17，4： | 5 |
| 4 | Hydrogen production through diesel steam reforming over rare-earth promoted Ni/γ-Al_2O_3 catalysts显示文摘Rare-earth (La, Ce, Yb) promoted Ni/γ-Al2O3 catalysts were prepared by impregnation method. Activity and carbon formation resistance of the prepared catalysts were evaluated under various reaction conditions. Catalyst characterizations with TG, TPR and H2 chemisorption were carried out to investigate the promoting mechanism. Experimental results show that rare-earth promoters, especially Yb promoter, obviously improve the activity and carbon formation resistance of Ni/γ-Al2O3 catalyst, and Yb-Ni catalyst shows even higher performance than several commercial catalysts. According to the characterization results, Yb promoter enhances the interaction between the active metal and support, thus increasing the active metal’s dispersion and improving its performance. Furthermore, the obvious difference in diesel conversion between Yb-Ni catalyst and others was shown in the temperature range of 450-550 °C, which would be the reason for its excellent carbon resistance. | Lihao Xu Wanliang Mi Qingquan Su | 2011 | Journal of Natural Gas Chemistry2011，20，3： | 4 |
| 5 | Influence of orifice-to-wall distance on synthetic jet vortex ring simpinging on a fixed wall显示文摘Two-dimensional particle image velocimetry (PIV) is used to investigate the influence of the orifice-to-wall distance on synthetic jet vortex rings impinging on a fixed wall. Both evolutions of vortical structures and statistical characteristics of flow fields at different orifice-to-wall distances are presented. It is found that different orifice-to-wall distances have different effects in terms of the vortex strength and impinging speed when the vortex rings are approaching the wall. The secondary vortex ring can be observed within the shear layer only when the dimensionless orifice-to-wall distance is close to or less than the dimensionless stroke length. Consequently, an appropriate orifice-to-wall distance plays a vital role in the sense of impingement effect. The statistical analysis of the flow field indicates that a wall jet forms after impingement, while both the decay rate of the maximum radial velocity and the spreading rate of the half-width decrease with the increasing orifice-to-wall distance. The non-dimensional wall jet velocity profiles at different orifice-to-wall distances all exhibit self-similar behaviors, which is consistent with the theoretical solution of the laminar wall jet. | XU Yang FENG LiHao | 2013 | Science China(Technological Sciences)2013，56，7： | 3 |
| 6 | STAR: an efficient coding scheme for correcting triple storage node failures 显示文摘 | Huang Cheng Xu Lihao | 2008 | IEEE Transaction on Computers2008，57，7： | 1 |
| 7 | Deterministic Voting in Distributed Systems Using Error-correcting Codes显示文摘 | Xu Lihao Bruck J | 1998 | IEEE Transactions on Parallel and Distributed Systems1998，9，8： | 1 |
| 8 | STAR: An efficient coding scheme for correcting triple storage node failures 显示文摘 | Huang Cheng Xu Lihao | 2008 | IEEE Transactions on Computers2008，57，7： | 1 |
| 9 | Preferential orientation of tracer spheroids in turbulent channel flow显示文摘Axis-symmetric spheroids, such as rod-like and disk-like particles, have been found to orient preferentially in near-wall turbulence by both experiment and numerical simulation. In current work we examined the orientation of inertialess spheroids in a turbulent channel flow at medium friction Reynolds number Reτ=100 given based on the half of channel height. Both elongated prolate spheroid and flat oblate spheroid are considered and further compared with the reference case of spherical particle. The statistical results show that in near wall region the prolate spheroids tend to align in the streamwise direction while the oblate spheroids prefer to orient in the wallnormal direction, which are consistent with earlier observation in low Reynolds number (Reτ=180)wall turbulence. Around the channel center we found that the orientation of spheroids is not fully isotropic, even though the fluid vorticity are almost isotropic. The mechanism that gives rise to such particle orientations in wall-turbulence has been found to be related to fluid Lagrangian stretching and compression (Zhao and Andersson 2016). Therefore, we computed the left Cauchy-Green strain tensor along Lagrangian trajectories of tracer spheroids in current flow field and analyzed the fluid Lagrangian stretching and compression. The results indicated that, similar to the earlier observations, the directions of the Lagrangian stretching and compression in near-wall region are in the streamwise and wall-normal directions, respectively. Furthermore, cross over the channel the prolate spheroids aligned with the direction of Lagrangian stretching but oblate spheroids oriented with the direction of Lagrangian compression. The weak anisotropy of orientations of fluid Lagrangian stretching and compression observed at the channel center could be the reason for the aforementioned modest anisotropic orientation of spheroids in channel central region. | Yucheng Jie Lihao Zhao Chunxiao Xu Helge I. Andersson | 2019 | Theoretical & Applied Mechanics Letters2019，9，3： | 1 |
| 10 | STAR:an efficient coding scheme for correcting triple storage node failure显示文摘 | Huang Cheng Xu Lihao | 2008 | IEEE Transactions on Computers2008，57，7： | 1 |
| 11 | Resource-efficient delivery of on-demand streaming data using UEP codes显示文摘 | XU Lihao | 2003 | IEEE Transactions on Communications2003，51，1： | 1 |
| 12 | X-code: MDS Array Codes with Optimal Encoding 显示文摘 | XU Lihao BRUCK J | 1999 | IEEE Transactions on Information Theory1999，45，1： | 1 |
| 13 | Low Density MDS Codes and Factors of Complete Graphs 显示文摘 | XU Lihao BOHOSSIAN V BRUCK J | 1999 | IEEE Transactions on Information Theory1999，45，6： | 1 |
| 14 | Computation-efficient multicast key distribution显示文摘 | Xu Lihao Huang Cheng | 2008 | IEEE Transactions on Parallel and Distributed Systems2008，19，5： | 1 |
| 15 | Deterministic voting in distributed systems using error-correcting codes显示文摘 | Bruck J | 1998 | IEEE Transactionson Paral-leland Distributed Systems1998，9，8： | 1 |
| 16 | Lighted up by hydrogen-bonding: luminescence behavior and applications of AIEgen-doped interpenetrating network hydrogel显示文摘The interaction between the aggregation-induced emissive(AIE) luminogens(AIEgen) and the polymer is the key scientific question in the design of functional AIE hydrogels. In this study, we report the AIE behavior of a poly(acrylic acid)(PAAc) and poly(acrylamide)(PAAm) interpenetrating network(IPN) hydrogel doped with tetra-cationic tetraphenylethene(TCTPE). The cationic AIEgen can bind with PAAc through ionic interaction, while PAAc and PAAm chains can associate with each other through hydrogen-bonds(H-bonds). These two interactions can restrict the intramolecular rotation and thus activate the luminescence of the AIEgen. The PAAc-PAAm H-bonds can be broken by increasing temperature or p H, restoring the intramolecular rotation of the AIEgen and quenching the fluorescence of the hydrogel. Therefore, the TCTPE-doped IPN hydrogel is designed as temperature-and p H-sensitive displayers which can record information imprinted by photo-printing or iono-printing with good switchability and reversibility. Another application of this TCTPE-doped hydrogel is demonstrated as a luminescent soft actuator, which has fast shape deformation and editable fluorescence pattern. The above results reveal a pathway to tune the emission behavior through tuning polymer-polymer and polymer-AIEgen interactions, which may inspire new design strategies of aggregation-induced emissive polymers and broaden their applications. | Mengdi Xu Luqin Hua Lihao Gong Jianlei Lu Jinhui Wang Chuanzhuang Zhao | 2021 | Science China Chemistry2021，64，10： | 1 |
| 17 | Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products显示文摘The mycelial bacterium Streptomyces is a workhorse for producing natural products,serving as a key source of drugs and other valuable chemicals.However,its complicated life cycle,silent biosynthetic gene clusters(BGCs),and poorly characterized metabolic mechanisms limit efficient production of natural products.There-fore,a metabolic engineering strategy,including traditional and emerging tools from different disciplines,was developed to further enhance natural product synthesis by Streptomyces.Here,current trends in systems metabolic engineering,including tools and strategies,are reviewed.Particularly,this review focuses on recent developments in the selection of methods for regulating the Streptomyces life cycle,strategies for the activation of silent gene clusters,and the exploration of regulatory mechanisms governing antibiotic production.Finally,future challenges and prospects are discussed. | Zuwei Xu Lihao Ji Wenxiu Tang Liang Guo Cong Gao Xiulai Chen Jia Liu Guipeng Hu Liming Liu | 2022 | Engineering Microbiology2022，2，2： | 0 |
| 18 | Multi-dimensional characteristic construction methods of computational materials under big data environment显示文摘Characteristic construction is an important part of material data analysis.In the big data environment,the de-velopment of material data science will have implications for multidimensional data analysis methods.Among these,the machine learning method for multidimensional data models can be widely applied to material data types,including element ratios,atomic compositions,electronic arrangements,molecular structures,and energy distributions.For high-throughput computing materials,it is recommended in material data science to judge and extract the main characteristics influencing material properties and predict novel functional materials us-ing the discovered laws.Consequently,we considere the characteristic construction,learning prediction,feature extraction,and high-order analysis of computational materials as the main research purposes,and construct a composite analysis model of the material system by combining data preprocessing,data mining,data evaluation,and knowledge representation as the main steps of data analysis.This demonstrates that a method to comprehen-sively judge the properties of materials by constructing the characteristics of materials in different dimensions is essential. | Lihao Chen Shuopu Wang Chen Zou Ben Xu Ke Bi | 2022 | ChemPhysMater2022，1，3： | 0 |
| 19 | Pepper variome reveals the history and key loci associated with fruit domestication and diversification显示文摘Pepper(Capsicum spp.)is an important vegetable crop that provides a unique pungent sensation when eaten.Through construction of a pepper variome map,we examined the main groups that emerged during domestication and breeding of C.annuum,their relationships and temporal succession,and the molecular events underlying the main transitions.The results showed that the initial differentiation in fruit shape and pungency,increase in fruit weight,and transition from erect to pendent fruits,as well as the recent appearance of large,blocky,sweet fruits(bell peppers),were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions.Furthermore,we identified Up,which encodes a BIG GRAIN protein involved in auxin transport,as a key domestication gene that controls erect vs pendent fruit orientation.The up mutation gained increased expression especially in the fruit pedicel through a 579-bp sequence deletion in its 5′upstream region,resulting in the phenotype of pendent fruit.The function of Up was confirmed by virus-induced gene silencing.Taken together,these findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop. | Yacong Cao Kang Zhang Hailong Yu Shumin Chen Donghui Xu Hong Zhao Zhenghai Zhang Yinqing Yang Xiaozhen Gu Xinyan Liu Haiping Wang Yaxin Jing Yajie Mei Xiang Wang Véronique Lefebvre Weili Zhang Yuan Jin Dongliang An Risheng Wang Paul Bosland Xixiang Li IIan Paran Baoxi Zhang Giovanni Giuliano Lihao Wang Feng Cheng | 2022 | Molecular Plant2022，15，11： | 0 |
| 20 | Relationship between wall shear stresses and streamwise vortices in turbulent flows over wavy boundaries显示文摘The relationship between wall shear stresses and near-wall streamwise vortices is investigated via a direct numerical simulation(DNS) of turbulent flows over a wavy boundary with traveling-wave motion. The results indicate that the wall shear stresses are still closely related to the near-wall streamwise vortices in the presence of a wave. The wave age and wave phase significantly affect the distribution of a two-point correlation coefficient between the wall shear stresses and streamwise vorticity. For the slow wave case of c/Um = 0.14, the correlation is attenuated above the leeward side while the distribution of correlation function is more elongated and also exhibits a larger vertical extent above the crest. With respect to the fast wave case of c/U_m=1.4, the distribution of the correlation function is recovered in a manner similar to that in the flat-wall case. In this case, the maximum correlation coefficient exhibits only slight differences at different wave phases while the vertical distribution of the correlation function depends on the wave phase. | Lihao WANG Weixi HUANG Chunxiao XU Lian SHEN Zhaoshun ZHANG | 2019 | Applied Mathematics and Mechanics(English Edition)2019，40，3： | 0 |
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