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| 1 | Surface modification of biodegradable magnesium and its alloys for biomedical applications显示文摘Magnesium and its alloys are being paid much attention recently as temporary implants,such as orthopedic implants and cardiovascular stents.However,the rapid degradation of them in physiological environment is a major obstacle preventing their wide applications to date,which will result in rapid mechanical integrity loss or even collapse of magnesium-based implants before injured tissues heal.Moreover,rapid degradation of the magnesium-based implants will also cause some adverse effects to their surrounding environment,such as local gas cavity around the implant,local alkalization and magnesium ion enrichment,which will reduce the integration between implant and tissue.So,in order to obtain better performance of magnesium-based implants in clinical trials,special alloy designs and surface modifications are prerequisite.Actually,when a magnesium-based implant is inserted in vivo,corrosion firstly happens at the implant-tissue interface and the biological response to implant is also determined by the interaction at this interface.So the surface properties,such as corrosion resistance,hemocompatibility and cytocompatibility of the implant,are critical for their in vivo performance.Compared with alloy designs,surface modification is less costly,flexible to construct multi-functional surface and can prevent addition of toxic alloying elements.In this review,we would like to summarize the current investigations of surface modifications of magnesium and its alloys for biomedical application.The advantages/disadvantages of different surface modification methods are also discussed as a suggestion for their utilization. | Peng Tian Xuanyong Liu | 2015 | Regenerative Biomaterials2015，2，2： | 22 |
| 2 | Combination types between graphene oxide and substrate affect the antibacterial activity显示文摘Duo to their superior physicochemical properties,graphene and its derivatives(GDs),such as graphene oxide(GO)and reduced graphene oxide(rGO),have attracted extensive research interests around the world.In recent years,antibacterial activities of GDs have aroused wide concern and substantial works have been done.However,the underlying antibacterial mechanisms still remain controversial.Antibacterial activities of GDs vary with various factors,such as size,number of layers,oxygen-containing groups,and experimental surroundings.We assume that combination types between graphene oxide and substrate may affect the antibacterial activity.Therefore,in this work,GO was fixed on the titanium surface with three kinds of combination types including drop with gravitational effects(GO-D),electrostatic interaction(GO-APS)and electrophoretic deposition(GO-EPD),and the antibacterial activities in vitro were systematically investigated.Results showed that combination types affected the ability of GO for preventing Staphylococcus aureus(S.aureus)from gathering,sharpness of wrinkles or edges and reactive oxygen spices(ROS)levels.Once S.aureus are in the form of separation without aggregation,GO can effectively interact with them and kill them with sharp wrinkles or edges and high ROS levels.GOEPD could effectively prevent S.aureus from gathering,own sharp wrinkles or edges and could generate higher ROS levels.As a result,GO-EPD exhibited optimal antibacterial activity against S.aureus,followed by GO-APS and GO-D. | Jiajun Qiu Lu Liu Hongqin Zhu Xuanyong Liu | 2018 | Bioactive Materials2018，3，3： | 7 |
| 3 | Osteogenesis, angiogenesis and immune response of Mg-Al layered double hydroxide coating on pure Mg显示文摘Layered double hydroxides(LDHs)are widely studied to enhance corrosion resistance and biocompatibility of Mg alloys,which are promising bone implants.However,the influence of LDH coating on the osteointegration of Mg implants lacks of a systematic study.In this work,Mg-Al LDH coating was prepared on pure Mg via hydrothermal treatment.The as-prepared Mg-Al LDH coated Mg exhibited better in vitro and in vivo corrosion resistance than bare Mg and Mg(OH)2 coated Mg.In vitro culture of mouse osteoblast cell line(MC3T3-E1)suggested that Mg-Al LDH coated Mg was more favorable for its osteogenic differentiation.In vitro culture of HUVECs revealed that cells cultured in the extract of Mg-Al LDH coated Mg showed superior angiogenic behaviors.More importantly,the immune response of Mg-Al LDH coated Mg was studied by in vitro culturing murine-derived macrophage cell line(RAW264.7).The results verified that Mg-Al LDH coated Mg could induce macrophage polarize to M2 phenotype(anti-inflammatory).Furthermore,the secreted factor in the macrophageconditioned culture medium of Mg-Al LDH group was more suitable for the bone differentiation of rat bone marrow stem cells(rBMSCs)and the angiogenic behavior of human umbilical vein endothelial cells(HUVECs).Finally,the result of femoral implantation suggested that Mg-Al LDH coated Mg exhibited better osteointegration than bare Mg and Mg(OH)2 coated Mg.With favorable in vitro and in vivo performances,Mg-Al LDH is promising as protective coating on Mg for orthopedic applications. | Shi Cheng Dongdong Zhang Mei Li Xuanyong Liu Yu Zhang Shi Qian Feng Peng | 2021 | Bioactive Materials2021，6，1： | 7 |
| 4 | Regulation of extracellular bioactive cations in bone tissue microenvironment induces favorable osteoimmune conditions to accelerate in situ bone regeneration显示文摘The design of orthopedic biomaterials has gradually shifted from“immune-friendly”to“immunomodulatory,”in which the biomaterials are able to modulate the inflammatory response via macrophage polarization in a local immune microenvironment that favors osteogenesis and implant-to-bone osseointegration.Despite the well-known effects of bioactive metallic ions on osteogenesis,how extracellular metallic ions manipulate immune cells in bone tissue microenvironments toward osteogenesis and subsequent bone formation has rarely been studied.Herein,we investigate the osteoimmunomodulatory effect of an extracellular bioactive cation(Mg^(2+))in the bone tissue microenvironment using custom-made poly lactic-co-glycolic acid(PLGA)/MgO-alendronate microspheres that endow controllable release of magnesium ions.The results suggest that the Mg^(2+)-controlled tissue microenvironment can effectively induce macrophage polarization from the M0 to M2 phenotype via the enhancement of anti-inflammatory(IL-10)and pro-osteogenic(BMP-2 and TGF-β1)cytokines production.It also generates a favorable osteoimmune microenvironment that facilitates the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.The in vivo results further verify that a large amount of bony tissue,with comparable bone mineral density and mechanical properties,has been generated at an early post-surgical stage in rat intramedullary bone defect models.This study demonstrates that the concept of in situ immunomodulated osteogenesis can be realized in a controlled magnesium tissue microenvironment. | Zhengjie Lin Danni Shen Weixiao Zhou Yufeng Zheng Tiantian Kong Xuanyong Liu Shuilin Wu Paul K.Chu Ying Zhao Jun Wu Kenneth M.C.Cheung Kelvin W.K.Yeung | 2021 | Bioactive Materials2021，6，8： | 5 |
| 5 | Enhanced physicochemical and biological properties of C/Cu dual ions implanted medical titanium显示文摘It is increasingly popular for titanium and its alloys to be utilized as the medical implants.However,their bioinert nature and lack of antibacterial ability limit their applications.In this work,by utilizing plasma immersion ion implantation and deposition(PIII&D)technology,the titanium surface was modified by C/Cu co-implantation.The mechanical property,corrosion resistance,antibacterial ability and cytocompatibility of modified samples were studied.Results indicate that after C/Cu co-implantation,copper nanoparticles were observed on the surface of titanium,and titanium carbide existed on the near surface region of titanium.The modified surface displayed good mechanical property and corrosion resistance.The Cu/C galvanic corrosion existed on the titanium surface implanted by C/Cu dual ions,and release of copper ions can be effectively controlled by the galvanic corrosion effect.Moreover,improved antibacterial performance of titanium surface can be achieved without cytotoxicity. | Chao Xia Xiaohan Ma Xianming Zhang Kunqiang Li Ji Tan Yuqin Qiao Xuanyong Liu | 2020 | Bioactive Materials2020，5，2： | 5 |
| 6 | Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts显示文摘Soft tissue sealing around implants acts as a barrier between the alveolar bone and oral environment,protecting implants from the invasion of bacteria or external stimuli.In this work,magnesium(Mg)and zinc(Zn)are introduced into titanium by plasma immersed ion implantation technology,and their effects on the behaviors of human gingival fibroblasts(HGFs)as well as the underlying mechanisms are investigated.Surface characterization confirms Mg and Zn exist on the surface in metallic and oxidized states.Contact angle test suggests that surface wettability of titanium changes after ion implantation and thus influences protein adsorption of surfaces.In vitro studies disclose that HGFs on Mg ion-implanted samples exhibit better adhesion and migration while cells on Zn ion-implanted samples have higher proliferation rate and amounts.The results of immunofluorescence staining and real-time reverse-transcriptase polymerase chain reaction(RT-PCR)suggest that Mg mainly regulates the motility and adhesion of HGFs through activating the MAPK signal pathway whereas Zn influences HGFs proliferation by triggering the TGF-βsignal pathway.The synergistic effect of Mg and Zn ions ensure that HGFs cultured on co-implanted samples possessed both high proliferation rate and motility,which are critical to soft tissue sealing of implants. | Lanyu Wang Qiming Luo Xianming Zhang Jiajun Qiu Shi Qian Xuanyong Liu | 2021 | Bioactive Materials2021，6，1： | 4 |
| 7 | Surface modification of titanium, titanium alloys, and related materials for biomedical applications显示文摘 | Xuanyong Liu Paul K. Chu Chuanxian Ding | 2004 | Materials Science & Engineering R2004，，3： | 3 |
| 8 | Mg-Fe LDH sealed PEO coating on magnesium for biodegradation control,antibacteria and osteogenesis显示文摘Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the present work,Mg-Fe LDH flms with different Fe contents were fabricated on PEO coating via a novel two-step method:frstly,the Fe OOH flms were prepared by immersing PEO sample in Fe^(2+)-containing solution,and then Mg-Fe LDH flms were formed by transforming the Fe OOH flms via a hydrothermal treatment in water.The highly-oriented LDH nano-sheets could enhance the anti-corrosion performance of PEO coating,which was proved by the results of electrochemical test,hydrogen evolution and corroded morphology.PEO/Mg-Fe LDH coatings showed a low hemolysis rate(less5%)than PEO coating.In addition,PEO/Mg-Fe LDH coatings were more favorable for cell adhesion and proliferation than PEO coating.Moreover,PEO/Mg-Fe LDH coatings showed good photothermal conversion property,which demonstrated the excellent rapid antibacterial effect under NIR light.In vitro culture of rat bone marrow stem cell(r BMSC)suggested that cells cultured in the extract of PEO/Mg-Fe LDH coatings had a better osteogenic activity.In vivo subcutaneous implantation test revealed that PEO/Mg-Fe LDH coatings exhibited good anti-corrosion and histocompatibility. | Dongdong Zhang Jielong Zhou Feng Peng Ji Tan Xianming Zhang Shi Qian Yuqin Qiao Yu Zhang Xuanyong Liu | 2022 | Journal of Materials Science & Technology2022，，10： | 3 |
| 9 | Synergistic effects of immunoregulation and osteoinduction of ds-block elements on titanium surface显示文摘Ds-block elements have been gaining increasing attention in the field of biomaterials modification,owing to their excellent biological properties,such as antibiosis,osteogenesis,etc.However,their function mechanisms are not well understood and conflicting conclusions were drawn by previous studies on this issue,which are mainly resulted from the inconsistent experimental conditions.In this work,three most widely used ds-block elements,copper,zinc,and silver were introduced on titanium substrate by plasma immersion ion implantation method to investigate the rule of ds-block elements in the immune responses.Results showed that the implanted samples could decrease the inflammatory responses compared with Ti sample.The trend of anti-inflammatory effects of macrophages on samples was in correlation with cellular ROS levels,which was induced by the implanted biomaterials and positively correlated with the number of valence electrons of ds-block elements.The co-culture experiments of macrophages and bone marrow mesenchymal stem cells showed that these two kinds of cells could enhance the anti-inflammation and osteogenesis of samples by the paracrine manner of PGE2.In general,in their steady states on titanium substrate(Cu2+,Zn2+,Ag),the ds-block elements with more valence electrons exhibit better anti-inflammatory and osteogenic effects.Moreover,molecular biology experiments indicate that the PGE2-related signaling pathway may contribute to the desired immunoregulation and osteoinduction capability of ds-block elements.These findings suggest a correlation between the number of valence electrons of ds-block elements and the relevant biological responses,which provides new insight into the selection of implanted ions and surface design of biomaterials. | Lan Chen Donghui Wang Jiajun Qiu Xianming Zhang Xingdan Liu Yuqin Qiao Xuanyong Liu | 2021 | Bioactive Materials2021，6，1： | 3 |
| 10 | Recent progress in superhydrophobic coating on Mg alloys:A general review显示文摘Magnesium(Mg)is a vital engineering material owing to its light weight and excellent mechanical properties.However,poor corrosion resistance limits its widely applications as well as its economic value.Hence,surface modification is essential for Mg and its alloys.Among the various coatings,superhydrophobic coating,which is inspired by nature,has received increasing attentions in the past decade.With a water contact angle larger than 150°,superhydrophobic coating can provide sufficient protection for Mg-based substrates.The model of superhydrophobic states and the protection mechanism of superhydrophobic coating are discussed in this review.Especially,the methods for fabricating superhydrophobic coatings on Mg alloys are reviewed.Meanwhile,some functional superhydrophobic coatings on Mg alloys are summarized.Finally,the challenges and future directions are proposed.We hope that this paper will provide a serviceable review for future research on superhydrophobic coatings on Mg alloys. | Feng Peng Dongdong Zhang Xuanyong Liu Yu Zhang | 2021 | Journal of Magnesium and Alloys2021，9，5： | 3 |
| 11 | FeeNeC single atom nanozymes with dual enzyme-mimicking activities for colorimetric detection of hydrogen peroxide and glutathione显示文摘H_(2)O_(2) and glutathione(GSH)are critical redox molecules in the organism.Abnormal levels of cellular H_(2)O_(2) and GSH are closely related to some diseases.Thus,it is imperative to detect H_(2)O_(2) and GSH effi-ciently.In this work,FeeNeC single atom nanozymes(SANs)with both peroxidase and oxidasemimicking activities were successfully prepared with the help of formamide condensation by one-step hydrothermal method.The FeeNeC SANs show excellent peroxidase-like activity,which possess a higher affinity for H_(2)O_(2) and 3,30,5,50-tetramethylbenzidine(TMB)than horseradish peroxidase(HRP).Then,based on the chromogenic reaction of TMB,a colorimetric biosensor to detect H_(2)O_(2) and GSH was developed.This biosensor has the linear ranges of 10-600 mmol/L for H_(2)O_(2) with a low detection limit of 4.360 mmol/L and 100-400 mmol/L for GSH with a low detection limit of 78.33 mmol/L.Besides,this colorimetric biosensor exhibited a good recovery of H_(2)O_(2) and GSH in diluted human serum.Finally,the FeeNeC SANs were encapsulated into agar gel to self-quantitatively detect GSH by naked eyes.This work provides a non-pyrolytic way to prepare SANs,which broadens the synthetic method of SANs and may promote the development of SANs for biosensor application. | Wei Lu Shuhan Chen Haifeng Zhang Jiajun Qiu Xuanyong Liu | 2022 | Journal of Materiomics2022，8，6： | 2 |
| 12 | Surface alloyed Ti–Zr layer constructed on titanium by Zr ion implantation for improving physicochemical and osteogenic properties显示文摘Titanium has been widely used as dental and orthopedic implant, while its corrosion resistance and osteogenic properties need to be further improved. Ti–Zr alloy is considered as one of promising titanium alloy with good corrosion resistance and biocompatibility. In this work, zirconium ions are implanted into the titanium surface by plasma immersion ion implantation(PIII) technology to construct Ti–Zr alloy layer on titanium surface with different proportions of Ti/Zr. The mechanical property, corrosion resistance and cytocompatibility of modified titanium were studied. Results indicated that Ti–Zr alloy layer on titanium exhibited good mechanical property and corrosion resistance. It could promote the cell spreading, proliferation, extracellular matrix(ECM) mineralization, and collagen secretion of r BMSCs. Besides, the zirconium ions released from different Ti–Zr alloy layers could promote the expressions of osteogenesis related genes, including alkaline phosphatase(ALP), type I collagen(Col-I), osteocalcin(OCN), osteopontin(OPN), and runt-related transcription factor 2(Runx2). The study provides reference for the design of Ti–Zr alloys and the surface modification of titanium. | Bangcheng Yan Ji Tan Donghui Wang Jiajun Qiu Xuanyong Liu | 2020 | Progress in Natural Science:Materials International2020，30，5： | 2 |
| 13 | Stepwise 3D-spatio-temporal magnesium cationic niche: Nanocomposite scaffold mediated microenvironment for modulating intramembranous ossification显示文摘The fate of cells and subsequent bone regeneration is highly correlated with temporospatial coordination of chemical,biological,or physical cues within a local tissue microenvironment.Deeper understanding of how mammalian cells react to local tissue microenvironment is paramount important when designing next generation of biomaterials for tissue engineering.This study aims to investigate that the regulation of magnesium cationic(Mg^2+)tissue microenvironment is able to convince early-stage bone regeneration and its mechanism undergoes intramembranous ossification.It was discovered that moderate Mg^2+content niche(~4.11 mM)led to superior bone regeneration,while Mg^2+-free and strong Mg^2+content(~16.44 mM)discouraged cell adhesion,proliferation and osteogenic differentiation,thereby bone formation was rarely found.When magnesium ions diffused into free Mg zone from concentrated zone in late time point,new bone formation on free Mg zone became significant through intramembranous ossification.This study successfully demonstrates that magnesium cationic microenvironment serves as an effective biochemical cue and is able to modulate the process of bony tissue regeneration.The knowledge of how a Mg^2+cationic microenvironment intertwines with cells and subsequent bone formation gained from this study may provide a new insight to develop the next generation of tissuerepairing biomaterials. | Jie Shen Bo Chen Xinyun Zhai Wei Qiao Shuilin Wu Xuanyong Liu Ying Zhao Changshun Ruan Haobo Pan Paul K.Chu Kenneth M.C.Cheung Kelvin W.K.Yeung | 2021 | Bioactive Materials2021，6，2： | 2 |
| 14 | Ferric oxide nanosheet-engineered Mg alloy for synergetic osteosarcoma photothermal/chemodynamic therapy显示文摘Osteosarcoma(OS)is a malignant tumor with a high rate of recurrence.Recently,biodegradable Mg-based implants have become a new therapeutic platform for bone-related diseases.However,poor biosafety and deficient intelligent tumor-killing ability of Mg-based implants are still the main challenges for the pre-cise treatment of OS.Herein,based on the excellent catalytic and photothermal conversion properties of nanozyme ferric oxide(Fe_(3)O_(4)),a novel two-step hydrothermal method for in situ preparation of Fe_(3)O_(4)nanosheets on the surface of plasma electrolytic oxidation(PEO)-treated Mg alloy using Mg-Fe layered double hydroxides(Mg-Fe LDH)as precursor was proposed.Compared with Mg alloy,there were no obvious corrosion cracks on the surface of Fe_(3)O_(4)nanosheets-coated Mg alloy(Fe_(3)O_(4)-NS)immersed in 0.9 wt.%NaCl for 14 days,which demonstrated the corrosion resistance of Mg alloy was significantly enhanced.Cytocompatibility experiments and hemolysis assay confirmed the great biocompatibility of Fe_(3)O_(4)-NS,especially,hemolysis ratio was lower than 1%.Meanwhile,Fe_(3)O_(4)-NS presented excellent cat-alytic oxidation capacity in the presence of H_(2)O_(2),and its temperature can significantly increase from 27℃to approximately 56℃under NIR irradiation.Therefore,intelligent responsive Fe_(3)O_(4)nanosheets-engineered Mg-based implants demonstrated excellent antitumor properties in vivo and in vitro due to their photothermal and chemodynamic synergetic effects.This study provides a novel approach for the preparation of Fe_(3)O_(4)coatings on the surface of Mg alloys and a new strategy for the treatment of OS. | Huihui Du Dongdong Zhang Ru Xu Juning Xie Shiwei Guan Shuhan Chen Feng Peng Shi Qian Xuanyong Liu | 2023 | Journal of Materials Science & Technology2023，，7： | 2 |
| 15 | Surface modification of titanium, titanium alloys, and related materials for biomedical applications显示文摘 | Xuanyong Liu Paul K. Chu Chuanxian Ding | 2004 | Materials Science & Engineering R2004，，3： | 2 |
| 16 | Chitosan@Puerarin hydrogel for accelerated wound healing in diabetic subjects by miR-29ab1 mediated inflammatory axis suppression显示文摘Wound healing is one of the major global health concerns in patients with diabetes.Overactivation of pro-inflammatory M1 macrophages is associated with delayed wound healing in diabetes.miR-29ab1 plays a critical role in diabetes-related macrophage inflammation.Hence,inhibition of inflammation and regulation of miR-29 expression have been implicated as new points for skin wound healing.In this study,the traditional Chinese medicine,puerarin,was introduced to construct an injectable and self-healing chitosan@puerarin(C@P)hydrogel.The C@P hydrogel promoted diabetic wound healing and accelerated angiogenesis,which were related to the inhibition of the miR-29 mediated inflammation response.Compared to healthy subjects,miR-29a and miR-29b1 were ectopically increased in the skin wound of the diabetic model,accompanied by upregulated M1-polarization,and elevated levels of IL-1βand TNF-α.Further evaluations by miR-29ab1 knockout mice exhibited superior wound healing and attenuated inflammation.The present results suggested that miR-29ab1 is essential for diabetic wound healing by regulating the inflammatory response.Suppression of miR-29ab1 by the C@P hydrogel has the potential for improving medical approaches for wound repair. | Xiaoling Zeng Baohui Chen Luping Wang Yingxiao Sun Zhao Jin Xuanyong Liu Liping Ouyang Yun Liao | 2023 | Bioactive Materials2023，，1： | 2 |
| 17 | Plasma-sprayed diopside coatings for biomedical applications显示文摘 | Weichang X Xuanyong L Xuebin Z | 2004 | Surface and Coatings Technology2004，185，7： | 1 |
| 18 | Puerarin@Chitosan composite for infected bone repair through mimicking the bio-functions of antimicrobial peptides显示文摘It is important to eliminate lipopolysaccharide(LPS)along with killing bacteria in periprosthetic joint infection(PJI)therapy for promoting bone repair due to its effect to regulate macrophages response.Although natural antimicrobial peptides(AMPs)offer a good solution,the unknown toxicity,high cost and exogenetic immune response hamper their applications in clinic.In this work,we fabricated a nanowire-like composite material,named P@C,by combining chitosan and puerarin via solid-phase reaction,which can finely mimic the bio-functions of AMPs.Chitosan,serving as the bacteria membrane puncture agent,and puerarin,serving as the LPS target agent,synergistically destroy the bacterial membrane structure and inhibit its recovery,thus endowing P@C with good antibacterial property.In addition,P@C possesses good osteoimmunomodulation due to its ability of LPS elimination and macrophage differentiation modulation.The in vivo results show that P@C can inhibit the LPS induced bone destruction in the Escherichia coli infected rat.P@C exhibits superior bone regeneration in Escherichia coli infected rat due to the comprehensive functions of its superior antibacterial property,and its ability of LPS elimination and immunomodulation.P@C can well mimic the functions of AMPs,which provides a novel and effective method for treating the PJI in clinic. | Liping Ouyang Baohui Chen Xingdan Liu Donghui Wang Yang Li Yun Liao Kelvin W.K.Yeung Xuanyong Liu | 2023 | Bioactive Materials2023，，3： | 1 |
| 19 | Optical and biological sensing capabilities of Au2S/ AuAgS coated gold nanorods 显示文摘 | Haowen Huang Xuanyong Liu Yunlong Zeng | 2009 | Biomaterials2009，30，： | 1 |
| 20 | Proliferation and gene expression of osteoblasts cultured in DMEM containing the ionic products of dicalcium silicate coating显示文摘 | Junying Sun Jianyou Li Xuanyong Liu Li Wei Guocheng Wang Fanhao Meng | 2009 | Biomedicine & Pharmacotherapy2009，，9： | 1 |
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