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您的检索式：作者名="XuefengWang"
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| 1 | Land use effects on soil organic carbon, nitrogen and salinity in saline-alkaline wetland显示文摘Land-use and soil management affects soil organic carbon (SOC) pools, nitrogen, salinity and the depth distribution. The objective of this study was to estimate land-use effects on the distribution of SOC, labile fractions C, nitrogen (N) and salinity in saline-alkaline wetlands in the middle reaches of the Heihe River Basin. Three land-use types were selected: intact saline-alkaline meadow wetland, artificial shrubbery (planting Tamarix) and farmland (cultivated for 18 years) of soils previously under meadow wetland. SOC, easily oxidized carbon, microbial biomass carbon, total N, NO3--N and salinity concentrations were measured. The results show that SOC and labile fraction carbon contents decreased significantly with increasing soil depth in the three land-use wetlands. The labile fraction carbon contents in the topsoil (0-20cm) in cultivated soils were significantly higher than that in intact meadow wetland and artificial shrubbery soil. The aboveground biomass and soil permeability were the primary influencing factors on the contents of SOC and the labile carbon in the intact meadow wetland and artificial shrubbery soil, however, the farming practice was a factor in cultivated soil. Agricultural measures can effectively reduce the salinity contents; however, it caused a significant increase of NO 3--N concentrations which posed a threat to groundwater quality in the study area. | WenJie Liu YongZhong Su Rong Yang XueFengWang XiaoYang | 2010 | Research in Cold and Arid Regions2010，2，3： | 6 |
| 2 | Development of a pyrF-based counterselectable system for targeted gene deletion in Streptomyces rimosus显示文摘Streptomyces produces many valuable and important biomolecules with clinical and pharmaceutical applications.The development of simple and highly efficient gene editing tools for genetic modification of Streptomyces is highly desirable.In this study,we developed a screening system for targeted gene knockout using a uracil auxotrophic host(ΔpyrF)resistant to the highly toxic uracil analog of 5-fluoroorotic acid(5-FOA)converted by PyrF,and a non-replicative vector pKC1132-pyrF carrying the complemented pyrF gene coding for orotidine-5'-phosphate decarboxylase.The pyrF gene acts as a positive selection and counterselection marker for recombinants during genetic modifications.Single-crossover homologous integration mutants were selected on minimal medium without uracil by reintroducing pyrF along with pKC1132-pyrF into the genome of the mutantΔpyrF at the targeted locus.Double-crossover recombinants were generated,from which the pyrF gene,plasmid backbone,and targeted gene were excised through homologous recombination exchange.These recombinants were rapidly screened by the counterselection agent,5-FOA.We demonstrated the feasibility and advantage of using this pyrF-based screening system through deleting the otcR gene,which encodes the cluster-situated regulator that directly activates oxytetracycline biosynthesis in Streptomyces rimosus M4018.This system provides a new genetic tool for investigating the genetic characteristics of Streptomyces species. | Yiying YANG Qingqing SUN Yang LIU Hanzhi YIN Wenping YANG Yang WANG Ying LIU Yuxian LI Shen PANG Wenxi LIU Qian ZHANG Fang YUAN Shiwen QIU Jiong LI XuefengWANG Keqiang FAN Weishan WANG Zilong LI Shouliang YIN | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，5： | 0 |
| 3 | In vitro studies on enrichment of dendritic cells and its role in antitumor immunity显示文摘 | LiZhang XuefengWang | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
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