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| 1 | Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template显示文摘 | Zhili Rong Shengyun Zhu Yang Xu Xuemei Fu | 2014 | Protein & Cell2014，5，4： | 9 |
| 2 | The immunogenicity of cells derived from induced pluripotent stem cells显示文摘与他们在文化经历无限的自强并且在身体区分进所有房间类型的能力,人的胚胎的干细胞(hESCs ) 为治疗保持大潜力当前不治之症。为针的绳索损害和有斑点的退化的二基于 hESC 的房间治疗被推进了进人的临床的试用。尽管有这快速的进步,基于 hESC 的房间治疗的一关键挑战是由接受者的导出 hESC 的房间的 allogeneic 免疫者拒绝。这个问题能被病人特定的体的房间的原子 reprogramming 被最近的突破与定义因素在导致的 pluripotent 干细胞(iPSCs ) 的技术减轻它能为房间治疗成为自体同源的房间的可更新的来源。然而,揭示反常 epigenetics, genomic 稳定性和 iPSCs 的 immunogenicity 的最近的研究在基于 iPSC 的治疗上提起了安全担心。与 iPSC 衍生物的 immunogenicity 有关的最近的调查结果将在这评论被总结。 | Xuemei Fu | 2014 | Cellular & Molecular Immunology2014，11，1： | 9 |
| 3 | CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway显示文摘With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9(CAS9)system has become the most widely used gene editing technology in biomedical research.In addition,significant progress has been made for the clinical development of CRISPR/CAS9 based gene therapies of human diseases,several of which are entering clinical trials.Here we report that CAS9 protein can function as a genome mutator independent of any exogenous guide RNA(gRNA)in human cells,promoting genomic DNA double-stranded break(DSB)damage and genomic instability.CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase(DNA-PK)complex and disrupts the interaction between KU86 and its kinase subunit,leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining(NHEJ)pathway.XCAS9 is a CAS9 variant with potentially higher fidelity and broader compatibility,and dCAS9 is a CAS9 variant without nuclease activity.We show that XCAS9 and dCAS9 also interact with KU86 and disrupt DNA DSB repair.Considering the critical roles of DNA-PK in maintaining genomic stability and the pleiotropic impact of DNA DSB damage responses on cellular proliferation and survival,our findings caution the interpretation of data involving CRISPR/CAS9-based gene editing and raise serious safety concerns of CRISPR/CAS9 system in clinical application. | Shuxiang Xu Jinchul Kim Qingshuang Tang Qu Chen Jingfeng Liu Yang Xu Xuemei Fu | 2020 | Protein & Cell2020，11，5： | 8 |
| 4 | Comprehensive Analysis of 9-year Running Data of A^2/O-MBR in Xi an Siyuan University显示文摘Since September 2011,A 2/O-MBR wastewater treatment plant in Xi'an Siyuan University has been successfully running without replacing any of membrane modules.A total of 6.661 million m^3 of campus wastewater has been treated,and 4.405 million m^3 of water reaching the national urban miscellaneous water standard has been reclaimed and reused.A total of 25 km pipeline,5 pits,scenery fountains,lakes,and 12 pump stations have been constructed for flushing toilets,irrigating green grass lawn and afforest,and cleaning hardened pavement.The design and construction of 5 pits,scenery fountains,and lakes are important to meet the national urban miscellaneous water standard under occasional phosphorus and/or nitrogen exceed concentration.The unit water cost is 4.19 yuan/m^3,including membrane modules depreciation and amortization.After the A 2/O-MBR system passes the membrane modules depreciation and amortization period,the unit water cost is 2.82 yuan/m^3.The water productivity of the membrane stack rises first at the beginning of operation,reaches the maximum value,and then decreases.The average annual transmembrane pressure difference increases by 1.12 kPa.The average VMD of industrial permeability decreases annually by 0.45 m^3. | Xuemei ZHANG Jingyuan HAO Qinghua MA Dong LI Bo FU Baolan QIN | 2020 | Meteorological and Environmental Research2020，11，5： | 6 |
| 5 | Magnetically Driven Superhydrophobic Polyurethane Sponge for High Efficiency Oil/Water Mixtures Separation显示文摘Magnetically driven super-hydrophobic materials were prepared by Fe3O4 nanoparticles and stearic acid,which were deposited on the surface of polyurethane sponges.The presence of the Fe3O4 nanoparticles makes the sponge have the magnetic,and the micro-nano hierarchical structure and hydrophobic functional groups lead to the sponge have excellent superhydrophobicity.The as-prepared sponge exhibited excellent absorption capacities for various oils and organic solvents ranging from 23.8 times to 86.7 times of its own weight.Moreover,the oil separation capacities still keep a high value after 50 cycles of squeezing the saturated absorbed as-prepared sponge.All of these satisfactory properties make the as-prepared sponge as a candidate of ideal absorbents for oily industrial wastewater and oil spills in oceans. | Xuemei Zhang Feng Fu Xiaoming Gao Xiufang Hou | 2019 | Journal of Bionic Engineering2019，16，1： | 6 |
| 6 | Effect of Carbon Addition on Microstructure and Properties of WC-Co Cemented Carbides显示文摘Based on a unique method to synthesize WC-Co composite powder by insitu reactions of metal oxides and carbon, the effects of the carbon addition in the initial powders on the phase constitution, microstructure and mechanical properties of the cemented carbides were investigated. It is found that with a suitable carbon addition the pure phase constitution can be obtained in the sintered bulk from the composite powder. The mechanical properties of the cemented carbides depend on the phase constitution and the WC grain structure. To obtain the excellent properties of the WC-Co bulk, it is important to obtain the pure phase constitution from the appropriate carbon addition in the initial powders and a suitable grain size. | Chongbin Wei Xiaoyan Song Jun Fu Xiaosen Lv Haibin Wang Yang Cao ShixianZhao Xuemei Liu | 2012 | Journal of Materials Science & Technology2012，28，9： | 5 |
| 7 | Functions of p53 in pluripotent stem cells显示文摘Pluripotent stem cells(PSCs)are capable of unlimited self-renewal in culture and differentiation into all functional cell types in the body,and thus hold great promise for regenerative medicine.To achieve their clinical potential,it is critical for PSCs to maintain genomic stability during the extended proliferation.The critical tumor suppressor p53 is required to maintain genomic stability of mammalian cells.In response to DNA damage or oncogenic stress,p53 plays multiple roles in maintaining genomic stability of somatic cells by inducing cell cycle arrest,apoptosis,and senescence to prevent the passage of genetic mutations to the daughter cells.p53 is also required to maintain the genomic stability of PSCs.However,in response to the genotoxic stresses,a primary role of p53 in PSCs is to induce the differentiation of PSCs and inhibit pluripotency,providing mechanisms to maintain the genomic stability of the self-renewing PSCs.In addition,the roles of p53 in cellular metabolism might also contribute to genomic stability of PSCs by limiting oxidative stress.In summary,the elucidation of the roles of p53 in PSCs will be a prerequisite for developing safe PSC-based cell therapy. | Xuemei Fu Shouhai Wu Bo Li Yang Xu Jingfeng Liu | 2020 | Protein & Cell2020，11，1： | 4 |
| 8 | A pH-sensitive supramolecular nanosystem with chlorin e6 and triptolide co-delivery for chemo-photodynamic combination therapy显示文摘The combination of Ce6,an acknowledged photosensitizer,and TPL,a natural anticancer agent,has been demonstrated as a useful strategy to reinforce the tumor growth suppression,as well as decrease the systemic side effects compared with their monotherapy.However,in view of the optimal chemo-photodynamic combination efficiency,there is still short of the feasible nanovehicle to steadily co-deliver Ce6 and TPL,and stimuli-responsively burst release drugs in tumor site.Herein,we described the synergistic antitumor performance of a pH-sensitive supramolecular nanosystem,mediated by the host–guest complexing betweenβ-CD and acid pH-responsive amphiphilic co-polymer mPEG-PBAE-mPEG,showing the shell–core structural micelles with the tightβ-CD layer coating.Both Ce6 and TPLwere facilely co-loaded into the spherical supramolecular NPs(TPL+Ce6/NPs)by one-step nanoprecipitation method,with an ideal particle size(156.0 nm),acid pH-responsive drug release profile,and enhanced cellular internalization capacity.In view of the combination benefit of photodynamic therapy and chemotherapy,as well as co-encapsulation in the fabricated pH-sensitive supramolecular NPs,TPL+Ce6/NPs exhibited significant efficacy to suppress cellular proliferation,boost ROS level,lower MMP,and promote cellular apoptosis in vitro.Particularly,fluorescence imaging revealed that TPL+Ce6/NPs preferentially accumulated in the tumor tissue area,with higher intensity than that of free Ce6.As expected,upon 650-nm laser irradiation,TPL+Ce6/NPs exhibited a cascade of amplified synergistic chemo-photodynamic therapeutic benefits to suppress tumor progression in both hepatoma H22 tumor-bearingmice and B16 tumor-bearingmice.More importantly,lower systemic toxicitywas found in the tumor-bearingmice treated with TPL+Ce6/NPs.Overall,the designed supramolecular TPL+Ce6/NPs provided a promising alternative approach for chemo-photodynamic therapy in tumor treatment. | Yihan Wu Jingjing Li Xuemei Zhong Jinfeng Shi Yanfen Cheng Chenglin He Jiaxin Li Liang Zou Chaomei Fu Meiwan Chen Jinming Zhang Huile Gao | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，2： | 4 |
| 9 | Therapeutic potential of bone marrow-derived mononuclear cells for experimental cerebral ischemia in mice显示文摘Bone marrow mononuclear cells (BMMCs) can be directly harvested from the donor, allowing for easier application compared with bone marrow mesenchymal stem cells. The present study hypothesized that BMMC transplantation could ameliorate cerebral ischemia in a mouse model. BMMCs were double-labeled with PKH26 and 4', 6-diamidino-2-phenylindole (DAPI), followed by infusion into mice via the tail vein to induce focal cerebral ischemia. At 14 days after transplantation, morphological and neurofunctional recovery were analyzed. PKH26 and DAPI double-positive BMMCs were detected in the cerebral hemisphere of all transplantation mice. Following BMMC administration, there was significant difference in neurofunctional recovery, but no significant difference in survival rates between BMMC-treated mice and other mice. These results demonstrate that transplanted BMMCs migrate to brain tissue and | Xuemei Zhang Fang Du Dan Yang Chunjiang Yu Xiangnan Huang Wei Liu Jin Fu | 2010 | Neural Regeneration Research2010，5，23： | 3 |
| 10 | Mercury in soil,vegetable and human hair in a typical mining area in China:Implication for human exposure显示文摘Concentrations of total mercury(T-Hg) and methylmercury(MeHg) in soil, vegetables, and human hair were measured in a mercury mining area in central China. T-Hg and MeHg concentrations in soil ranged from 1.53 to 1054.97 mg/kg and 0.88 to 46.52 μg/kg, respectively.T-Hg concentrations was correlated with total organic carbon(TOC) content(R^2= 0.50, p<0.01)and pH values(R^2= 0.21, p<0.05). A significant linear relationship was observed between MeHg concentrations and the abundance of sulfate-reducing bacteria(SRB)(R^2= 0.39, p<0.05) in soil.Soil incubation experiments amended with specific microbial stimulants and inhibitors showed that Hg methylation was derived from SRB activity. T-Hg and MeHg concentrations in vegetables were 24.79 – 781.02 μg/kg and 0.01 – 0.18 μg/kg, respectively; levels in the edible parts were significantly higher than in the roots(T-Hg: p<0.05; MeHg: p<0.01). Hg species concentrations in rhizosphere soil were positively correlated to those in vegetables(p<0.01), indicating that soil was an important source of Hg in vegetables. Risk assessment indicated that the consumption of vegetables could result in higher probable daily intake(PDI) of T-Hg than the provisional tolerable daily intake(PTDI) for both adults and children. In contrast, the PDI of MeHg was lower than the reference dose. T-Hg and MeHg concentrations in hair samples ranged from 1.57 to 12.61 mg/kg and 0.04 to 0.94 mg/kg, respectively, and MeHg concentration in hair positively related to PDI of MeHg via vegetable consumption(R^2= 0.39, p<0.05), suggesting that vegetable may pose health risk to local residents. | Qin Jia Xuemei Zhu Yaqiong Hao Ziliang Yang Qi Wang Haihui Fu Hongjin Yu | 2018 | Journal of Environmental Sciences2018，30，6： | 3 |
| 11 | QTL analysis across multiple environments reveals promising chromosome regions associated with yield-related traits in maize under drought conditions显示文摘Drought is one of the most critical abiotic stresses influencing maize yield. Improving maize cultivars with drought tolerance using marker-assisted selection requires a better understanding of its genetic basis. In this study, a doubled haploid(DH) population consisting of 217 lines was created by crossing the inbred lines Han 21(drought-tolerant) and Ye 478(drought-sensitive). The population was genotyped with a 6 K SNP assay and 756 SNP(single nucleotide polymorphism) markers were used to construct a linkage map with a length of 1344 c M. Grain yield(GY), ear setting percentage(ESP), and anthesis–silking interval(ASI) were recorded in seven environments under well-watered(WW) and water-stressed(WS) regimes. High phenotypic variation was observed for all traits under both water regimes. Using the LSMEAN(least-squares mean) values from all environments for each trait, 18 QTL were detected, with 9 associated with the WW and 9 with the WS regime. Four chromosome regions,Chr. 3: 219.8–223.7 Mb, Chr. 5: 191.5–194.7 Mb, Chr. 7: 132.2–135.6 Mb, and Chr. 10: 88.2–89.4 Mb, harbored at least 2 QTL in each region, and QTL co-located in a region inherited favorable alleles from the same parent. A set of 64 drought-tolerant BC_3F_6 lines showed preferential accumulation of the favorable alleles in these four regions, supporting an association between the four regions and maize drought tolerance. QTL-by-environment interaction analysis revealed 28 ed QTL(environment-dependent QTL) associated with the WS regime and 22 associated with the WW regime for GY, ESP, and ASI. All WS QTL and55.6% of WW QTL were located in the ed QTL regions. The hotspot genomic regions identified in this work will support further fine mapping and marker-assisted breeding of drought-tolerant maize. | Xinmin Hu Guihua Wang Xuemei Du Hongwei Zhang Zhenxiang Xu Jie Wang Guo Chen Bo Wang Xuhui Li Xunji Chen Junjie Fu Jun Zheng Jianhua Wang Riliang Gu Guoying Wang | 2021 | The Crop Journal2021，9，4： | 3 |
| 12 | The deubiquitinase OTUD1 inhibits colonic inflammation by suppressing RIPK1-mediated NF-κB signaling显示文摘The E3 ubiquitin ligase(E3)-mediated ubiquitination and deubiquitinase(DUB)-mediated deubiquitination processes are closely associated with the occurrence and development of colonic inflammation.Ovarian tumor deubiquitinase 1(OTUD1)is involved in immunoregulatory functions linked to infectious diseases.However,the effect of OTUD1 on intestinal immune responses during colonic inflammatory disorders such as inflammatory bowel disease(IBD)remains unclear.Here,we show that loss of OTUD1 in mice contributes to the pathogenesis of dextran sulfate sodium(DSS)-induced colitis via excessive release of proinflammatory cytokines.In addition,bone marrow transplantation experiments revealed that OTUD1 in hematopoietic cells plays a dominant role in protection against colitis.Mechanistically,OTUD1 physically interacts with receptor-interacting serine/threonine-protein kinase 1(RIPK1)and selectively cleaves K63-linked polyubiquitin chains from RIPK1 to inhibit the recruitment of NF-κB essential modulator(NEMO).Moreover,the expression of OTUD1 in mucosa samples from ulcerative colitis(UC)patients was lower than that in mucosa samples from healthy controls.Furthermore,we demonstrate that the UC-associated OTUD1 G430V mutation abolishes the ability of OTUD1 to inhibit RIPK1-mediated NF-κB activation and intestinal inflammation.Taken together,our study unveils a previously unexplored role of OTUD1 in moderating intestinal inflammation by inhibiting RIPK1-mediated NF-κB activation,suggesting that the OTUD1-RIPK1 axis could be a potential target for the treatment of IBD. | Bo Wu Lihua Qiang Yong Zhang Yesheng Fu Mengyuan Zhao Zehui Lei Zhe Lu Yan-Ge Wei Hongmiao Dai Yingwei Ge Mingqiu Liu Xuemei Zhou Zhiqiang Peng Hongchang Li Chun-Ping Cui Jing Wang Hui Zheng Cui Hua Liu Lingqiang Zhang | 2022 | Cellular & Molecular Immunology2022，19，2： | 3 |
| 13 | Recent Progress in Atmospheric Chemistry Research in China: Establishing a Theoretical Framework for the “Air Pollution Complex”显示文摘Atmospheric chemistry research has been growing rapidly in China in the last 25 years since the concept of the“air pollution complex”was first proposed by Professor Xiaoyan TANG in 1997.For papers published in 2021 on air pollution(only papers included in the Web of Science Core Collection database were considered),more than 24000 papers were authored or co-authored by scientists working in China.In this paper,we review a limited number of representative and significant studies on atmospheric chemistry in China in the last few years,including studies on(1)sources and emission inventories,(2)atmospheric chemical processes,(3)interactions of air pollution with meteorology,weather and climate,(4)interactions between the biosphere and atmosphere,and(5)data assimilation.The intention was not to provide a complete review of all progress made in the last few years,but rather to serve as a starting point for learning more about atmospheric chemistry research in China.The advances reviewed in this paper have enabled a theoretical framework for the air pollution complex to be established,provided robust scientific support to highly successful air pollution control policies in China,and created great opportunities in education,training,and career development for many graduate students and young scientists.This paper further highlights that developing and low-income countries that are heavily affected by air pollution can benefit from these research advances,whilst at the same time acknowledging that many challenges and opportunities still remain in atmospheric chemistry research in China,to hopefully be addressed over the next few decades. | Tong ZHU Mingjin TANG Meng GAO Xinhui BI Junji CAO Huizheng CHE Jianmin CHEN Aijun DING Pingqing FU Jian GAO Yang GAO Maofa GE Xinlei GE Zhiwei HAN Hong HE Ru-Jin HUANG Xin HUANG Hong LIAO Cheng LIU Huan LIU Jianguo LIU Shaw Chen LIU Keding LU Qingxin MA Wei NIE Min SHAO Yu SONG Yele SUN Xiao TANG Tao WANG Tijian WANG Weigang WANG Xuemei WANG Zifa WANG Yan YIN Qiang ZHANG Weijun ZHANG Yanlin ZHANG Yunhong ZHANG Yu ZHAO Mei ZHENG Bin ZHU Jiang ZHU | 2023 | Advances in Atmospheric Sciences2023，40，8： | 2 |
| 14 | Using a novel cellular platform to optimize CRISPR/CAS9 technology for the gene therapy of AIDS显示文摘 | Jingjin He Thanutra Zhang Xuemei Fu | 2017 | Protein & Cell2017，8，11： | 2 |
| 15 | Structural isomer and high-yield of Pt1Ag28 nanoduster via one-pot chemical wet method显示文摘In order to understand the structure-property correlation and explore the application of metal nanoclusters, it is important and intriguing to determine their crystal structure and obtain high-yield. At the same time, this is also a challenge in nanoscience and technology. Here, we report the highly efficient synthesis of Pt1Ag28 nanocluster via one-pot chemical wet method. The crystal structure of Pt1Ag28 nanocluster was determined by X-ray crystallography to be a face centered cubic (FCC) kernel. This novel structure is the structural isomerization of Pt1Ag28 nanocluster reported before. This phenomenon is first discovered in the synthesis of alloy nanoclusters. In addition, Pt1Ag28 nanocluster has high yield and exhibits potential optics in the near infrared (NIR) fluorescent imaging. The time-dependent density functional theory (TD-DFT) calculation implied that the optical property of Pt1Ag28 was sensitive to its structure. This work provides a simple method to synthesize alloy nanoclusters with structural isomerization. | Xinzhang Lin Chao Liu Keju Sun Ren'an Wu Xuemei Fu Jiahui Huang | 2019 | Nano Research2019，12，2： | 1 |
| 16 | 查看详情显示文摘 | Zhou Fu Zhao Xuemei Lu Zhonghua | | 0，，10： | 1 |
| 17 | Synthesis of c-MnOOH nanorods and their isomorphous transformation into β-MnO2 and α-Mn2O3 nanorods显示文摘 | Zhou Fu Zhao Xuemei Yuan Cunguang | 2007 | Materials Science2007，42，： | 1 |
| 18 | Study of ocular pharmacokinetics of in situ gel system for S( - ) satropane evaluated bymicrodialysis 显示文摘 | Fu Jun Feng Xuemei Yuan Haihong | 2008 | J Pharmac Biomed Anal2008，8，3： | 1 |
| 19 | OTUB1 promotes osteoblastic bone formation through stabilizing FGFR2显示文摘Bone homeostasis is maintained by the balance between osteoblastic bone formation and osteoclastic bone resorption.Dysregulation of this process leads to multiple diseases,including osteoporosis.However,the underlying molecular mechanisms are not fully understood.Here,we show that the global and conditional osteoblast knockout of a deubiquitinase Otub1 result in low bone mass and poor bone strength due to defects in osteogenic differentiation and mineralization.Mechanistically,the stability of FGFR2,a crucial regulator of osteogenesis,is maintained by OTUB1.OTUB1 attenuates the E3 ligase SMURF1-mediated FGFR2 ubiquitination by inhibiting SMURF1’s E2 binding.In the absence of OTUB1,FGFR2 is ubiquitinated excessively by SMURF1,followed by lysosomal degradation.Consistently,adeno-associated virus serotype 9(AAV9)-delivered FGFR2 in knee joints rescued the bone mass loss in osteoblast-specific Otub1-deleted mice.Moreover,Otub1 mRNA level was significantly downregulated in bones from osteoporotic mice,and restoring OTUB1 levels through an AAV9-delivered system in ovariectomy-induced osteoporotic mice attenuated osteopenia.Taken together,our results suggest that OTUB1 positively regulates osteogenic differentiation and mineralization in bone homeostasis by controlling FGFR2 stability,which provides an optical therapeutic strategy to alleviate osteoporosis. | Qiong Zhu Yesheng Fu Chun-Ping Cui Yi Ding Zhikang Deng Chao Ning Fan Hu Chen Qiu Biyue Yu Xuemei Zhou Guan Yang Jiang Peng Weiguo Zou Cui Hua Liu Lingqiang Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 1 |
| 20 | Synthesis and crystal structure of a novel charge transfer salt,(TMT-TTF), 显示文摘 | XUEMEI LIU XINZHONG LUtFENG FU? | 2005 | Journal Molecular Structure2005，751，123： | 1 |
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