|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model显示文摘Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by itself was difficult to meet the need of SCI functional recovery.The basic fibroblast growth factor(bFGF)administration significantly promotes functional recovery after organ injuries.Here,using a rat model of T9 hemisected SCI,we aimed at assessing the repair capacity of implantation of collagen scaffold(CS)modified by collagen binding bFGF(CBD-bFGF).The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI.The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group,when evaluated by the 21-point Basso-Beattie-Bresnahan(BBB)score and footprint analysis.Both hematoxylin and eosin(H&E)and immunohistochemical staining of neurofilament(NF)and glial fibrillary acidic protein(GFAP)demonstrated that fibers were guided to grow through the implants.These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery. | SHI Qin GAO Wei HAN XingLong ZHU XueSong SUN Jie XIE Fang HOU XiangLin YANG HuiLin DAI JianWu CHEN Liang | 2014 | Science China(Life Sciences)2014，57，2： | 23 |
| 2 | NUMERICAL SIMULATION OF TWO-POINT CONTACT BETWEEN WHEEL AND RAIL显示文摘有卷车轮栏杆的磨擦的有弹性塑料的接触问题用 FE 参量的二次的编程方法被解决。因此,复杂有弹性塑料的接触问题能与高精确性和效率是计算的,当赫的假设和有弹性的半空间假设被避免时。基于车轮栏杆的‘ o ne-point' 接触计算,‘ t wo-point' 接触的计算模型被证实并且推测车轮凸缘是否接近栏杆。在‘ t wo-point' 接触的情况中,车轮的变化法律 -- 栏杆接触在各种各样的负担诉讼中被介绍并且联系力量小心地被分析。车轮凸缘的主要原因穿并且责骂方面穿被发现。车轮凸缘的润滑油计算模型被构造。没有润滑油,在车轮凸缘之间的接触力量和栏杆减少,与结果作比较,它为减少车轮栏杆的 wear 是有益的。 | Jun Zhang Shouguang Sun Xuesong Jin | 2009 | Acta Mechanica Solida Sinica2009，22，4： | 8 |
| 3 | Emission factors, ozone and secondary organic aerosol formation potential of volatile organic compounds emitted from industrial biomass boilers显示文摘To evaluate the potential benefits of biomass use for air pollution control, this paper identified and quantified the emissions of major reactive organic compounds anticipated from biomass-fired industrial boilers. Wood pellets(WP) and straw pellets(SP) were burned to determine the volatile organic compound emission profiles for each biomass-boiler combination. More than 100 types of volatile organic compounds(VOCs) were measured from the two biomass boilers. The measured VOC species included alkanes, alkenes and acetylenes, aromatics, halocarbons and carbonyls. A single coal-fired boiler(CB) was also studied to provide a basis for comparison. Biomass boiler 1(BB1) emitted relatively high proportions of alkanes(28.9%–38.1% by mass) and alkenes and acetylenes(23.4%–40.8%),while biomass boiler 2(BB2) emitted relatively high proportions of aromatics(27.9%–29.2%)and oxygenated VOCs(33.0%–44.8%). The total VOC(TVOC) emission factors from BB1(128.59–146.16 mg/kg) were higher than those from BB2(41.26–85.29 mg/kg). The total ozone formation potential(OFP) ranged from 6.26 to 81.75 mg/m^3 with an average of 33.66 mg/m^3 for the two biomass boilers. The total secondary organic aerosol potential(SOAP) ranged from 61.56 to 211.67 mg/m^3 with an average of 142.27 mg/m^3 for the two biomass boilers.The emission factors(EFs) of TVOCs from biomass boilers in this study were similar to those for industrial coal-fired boilers with the same thermal power. These data can supplement existing VOC emission factors for biomass combustion and thus enrich the VOC emission inventory. | Chunmei Geng Wen Yang Xuesong Sun Xinhu Wang Zhipeng Bai Xia Zhang | 2019 | Journal of Environmental Sciences2019，31，9： | 8 |
| 4 | Famitinib in combination with concurrent chemoradiotherapy in patients with locoregionally advanced nasopharyngeal carcinoma: a phase 1, open-label, dose-escalation Study显示文摘Background:Famitinib is a tyrosine kinase inhibitor against multiple targets,including vascular endothelial growth factor receptor 2/3,platelet-derived growth factor receptor,and stem cell factor receptor(c-kit).Previous studies have demonstrated anti-tumour activities of famitinib against a wide variety of advanced-stage solid cancers.We aimed to determine the safety and efficacy of famitinib with concurrent chemoradiotherapy(CCRT)in patients with locoregionally advanced nasopharyngeal carcinoma(NPC).We also evaluated the feasibility of contrast-enhanced ultrasound(D-CEUS)as a predictor of early tumour response to famitinib and to correlate functional parameters with clinical efficacy.Methods:The trial was conducted in subjects with stage III or IVa-b NPC using a 3+3 design of escalating fami-tinib doses.Briefly,subjects received 2 weeks of famitinib monotherapy followed by 7 weeks of famitinib plus CCRT.D-CEUS of the neck lymph nodes was performed at day 0,8 and 15 after famitinib was administered before starting concurrent chemoradiotherapy.End points included safety,tolerability and anti-tumour activity.Results:Twenty patients were enrolled(six each for 12.5,16.5 and 20 mg and two for 25 mg).Two patients in the 25 mg cohort developed dose-limiting toxicities,including grade 4 thrombocytopenia and grade 3 hypertension.The most common grade 3/4 adverse events were leukopenia,neutropenia and radiation mucositis.D-CEUS tests showed that more than 60%of patients achieved a perfusion parameter response after 2 weeks taking famitinib alone,and the parameter response was associated with disease improvement.In the famitinib monotherapy stage,three patients(15%)showed partial responses.The complete response rate was 65%at the completion of treatment and 95%3 months after the treatment ended.After a median follow-up of 44 months,the 3-year progression-free survival(PFS)and distant metastasis-free survival were 70%and 75%,respectively.Subjects with a decrease of perfusion parameter response,such as peak intensity decreased at least 30%after 1 week of famitinib treatment,had higher 3-year PFS(90.9%vs.44.4%,95%CI 73.7%-100%vs.11.9%-76.9%,P<0.001)than those with an increase or a reduction of less than 30%.Conclusions:The recommended famitinib dose for phase II trial is 20 mg with CCRT for patients with local advanced NPC.D-CEUS is a reliable and early measure of efficacy for famitinib therapies.Further investigation is required to confirm the effects of famitinib plus chemoradiotherapy. | Qiuyan Chen Linquan Tang Na Liu Feng Han Ling Guo Shanshan Guo Jianwei Wang Huai Liu Yanfang Ye Lu Zhang Liting Liu Pan Wang Yingqin Li Qingmei He Xiaoqun Yang Qingnan Tang Yang Li YuJing Liang XueSong Sun Chuanmiao Xie Yunxian Mo Ying Guo Rui Sun Haoyuan Mo Kajia Cao Xiang Guo Musheng Zeng Haiqiang Mai Jun Ma | 2018 | Cancer Communications2018，38，1： | 6 |
| 5 | Knockdown of S-phase Kinase-associated Protein-2 Expression in MCF-7 Inhibits Cell Growth and Enhances the Cytotoxic Effects of Epirubicin显示文摘S 阶段联系 kinase 的 protein-2 (Skp2 ) 在调停 ubiquitin 的解朊作用起一个关键作用,导致房间从的前进一对一个 proliferative 状态静止。Skp2 完了在许多肿瘤表示了并且相反地与 p27 表示相关,包括乳癌。在这研究,我们使用了小介入的 RNA (siRNAs ) 在人的乳癌 MCF-7 细胞禁止 Skp2 表示并且如果击倒,从而由 RNA 干扰(RNAi ) Skp2 调查将禁止乳癌细胞生长并且在 MCF-7 细胞上影响 epirubicin 的效果。三 Skp2 siRNA 构造被重新结合并且短暂地 transfected 人乳癌 MCF-7 房间。Skp2 的下面规定被反向的抄写聚合酶链反应和蛋白质印迹分析证实。我们证明 Skp2 siRNA 转染减少了 Skp2 蛋白质并且在 MCF-7 房间导致了 p27 蛋白质的累积。Skp2 siRNA 在 vitro 并且在 vivo 禁止了房间增长。由 RNAi 的 Skp2 击倒在 vitro 的增加的细胞的 apoptosis。有治疗进一步与 epirubicin 跟随的 Skp2 siRNA 的治疗禁止了癌症房间线的增长,与 epirubicin 跟随的控制 siRNA 相比。因此, Skp2 击倒由 RNAi 的表示禁止乳癌细胞生长并且提高 epirubicin 的效果。Skp2 的调停 siRNA 的基因 silencing 能是为在规定下面的 p27 的抑制的新奇癌症基因治疗。 | Lichun SUN Li CAI Yan YU Qingwei MENG Xuesong CHENG Yanbin ZHAO Guangjie SUI Fengmin ZHANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，12： | 6 |
| 6 | S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer显示文摘Background:The transforming growth factor-β(TGF-β)pathway plays a pivotal role in inducing epithelial-mesenchymal transition(EMT),which is a key step in cancer invasion and metastasis.However,the regulatory mechanism of TGF-βin inducing EMT in colorectal cancer(CRC)has not been fully elucidated.In previous studies,it was found that S100A8 may regulate EMT.This study aimed to clarify the role of S100A8 in TGF-β-induced EMT and explore the underlying mechanism in CRC.Methods:S100A8 and upstream transcription factor 2(USF2)expression was detected by immunohistochemistry in 412 CRC tissues.Kaplan-Meier survival analysis was performed.In vitro,Western blot,and migration and invasion assays were performed to investigate the effects of S100A8 and USF2 on TGF-β-induced EMT.Mouse metastasis models were used to determine in vivo metastasis ability.Luciferase reporter and chromatin immunoprecipitation assay were used to explore the role of USF2 on S100A8 transcription.Results:During TGF-β-induced EMT in CRC cells,S100A8 and the transcription factor USF2 were upregulated.S100A8 promoted cell migration and invasion and EMT.USF2 transcriptionally regulated S100A8 expression by directly binding to its promoter region.Furthermore,TGF-βenhanced the USF2/S100A8 signaling axis of CRC cells whereas extracellular S100A8 inhibited the USF2/S100A8 axis of CRC cells.S100A8 expression in tumor cells was associated with poor overall survival in CRC.USF2 expression was positively related to S100A8 expression in tumor cells but negatively related to S100A8-positive stromal cells.Conclusions:TGF-βwas found to promote EMT and metastasis through the USF2/S100A8 axis in CRC while extracellular S100A8 suppressed the USF2/S100A8 axis.USF2 was identified as an important switch on the intracellular and extracellular S100A8 feedback loop. | Si Li Jun Zhang Senmi Qian Xuesong Wu Liang Sun Tianyi Ling Yao Jin Wenxiao Li Lichao Sun Maode Lai Fangying Xu | 2021 | Cancer Communications2021，41，2： | 5 |
| 7 | Characteristics of particulate-bound polycyclic aromatic hydrocarbons emitted from industrial grade biomass boilers显示文摘Polycyclic aromatic hydrocarbons(PAHs) are carcinogenic or mutagenic and are important toxic pollutants in the flue gas of boilers. Two industrial grade biomass boilers were selected to investigate the characteristics of particulate-bound PAHs: one biomass boiler retro-fitted from an oil boiler(BB1) and one specially designed(BB2) biomass boiler. One coal-fired boiler was also selected for comparison. By using a dilution tunnel system, particulate samples from boilers were collected and 10 PAH species were analyzed by gas chromatography–mass spectrometry(GC–MS). The total emission factors(EFs) of PAHs ranged from 0.0064 to0.0380 mg/kg, with an average of 0.0225 mg/kg, for the biomass boiler emission samples. The total PAH EFs for the tested coal-fired boiler were 1.8 times lower than the average value of the biomass boilers. The PAH diagnostic ratios for wood pellets and straw pellets were similar.The ratio of indeno(1,2,3-cd)pyrene/[indeno(1,2,3-cd)pyrene + benzo(g,h,i)perylene] for the two biomass boilers was lower than those of the reference data for other burning devices, which can probably be used as an indicator to distinguish the emission of biomass boilers from that of industrial coal-fired boilers and residential stoves. The toxic potential of the emission from wood pellet burning was higher than that from straw pellet burning, however both of them were much lower than residential stove exhausts. | Xiaoyang Yang Chunmei Geng Xuesong Sun Wen Yang Xinhua Wang Jianhua Chen | 2016 | Journal of Environmental Sciences2016，28，2： | 4 |
| 8 | Latest Zircon U-Pb Geochronology of the Huoshiling Formation Volcanic Rocks in the Southeastern Margin of the Songliao Basin显示文摘Objective The Huoshiling Formation is the earliest volcanic stratum in the Songliao Basin,composed mainly of intermediate-basic volcanic rocks,with rare fossils.The geological age of this formation has been controversial for long.The accurate dating is not only the basis | WANG Chenglong ZHANG Meisheng SUN Kai WANG Yini LI Xiaobo LIU Xuesong | 2017 | Acta Geologica Sinica(English Edition)2017，91，5： | 4 |
| 9 | Recent movement changes of main fault zones in the Sichuan-Yunnan region and their relevance to seismic activity显示文摘The Wenchuan earthquake occurred near the 'triple junction' linking the Bayan Har block, the South China block, and the Sichuan-Yunnan rhombic block, and its influences on the surrounding blocks and the main fault zones in the Sichuan-Yunnan region, i.e., the block boundary zone, cannot be ignored. In this paper, changes of movement and stress of the fault zones before and after a strong earthquake were simulated based on the GPS repetition survey results recently obtained during 1999–2007, 2009–2011, and 2011–2013 with a two-dimensional finite-element contact model and the 'block- loading' method. The results show that, before the Wenchuan earthquake, the movement of the Longmenshan fault zone was very slow and its compressive stress accumulated rapidly; after the Wenchuan earthquake, movements toward the E-SSE direction of the Bayan Har, southwestern Yunnan, and rhombic blocks were enhanced, and the dextral and horizontal compressive speeds and annual accumulative compressive stress of the Longmenshan fault zone increased markedly by factors of 4.5, 2.1, and 2.5, respectively. The southern Xianshuihe, Anninghe, Zemuhe, Daliangshan, and Lijiang-Xiaojinhe fault zones accumulated compressive stress rapidly, forming enhanced compressive stress zones along a NE strike crossing the central part of the Sichuan-Yunnan region. The tensional movement of the Xianshuihe fault zone was enhanced and the slip movement in the central part of the zone was reversed in a short time. The changes are tightly related to the medium-intensity earthquakes that occurred during the same period in this region, revealing that the spatial migration of seismic activity is related to changes of movement of the blocks. | LIU Xia MA Jin DU XueSong ZHU Shuang LI LaYue SUN DongYing | 2016 | Science China Earth Sciences2016，59，6： | 4 |
| 10 | Identification of Overlapping Protein Complexes Using Structural and Functional Information of PPI Network显示文摘An identification algorithm of overlapping protein complexes is put forward by simultaneously considering the topological structural and biological functional information of Protein-protein interaction(PPI) network.Main works include: constructing the edge weight of weighted PPI network on the basis of structural and functional information of PPI network to more accurately describe the correlation between protein vertices; improving the Newman algorithm to make it applicable to weighted PPI network and thus to identify overlapping protein complexes; and providing the denoising criteria based on the structural and function information of PPI network: connections which have no contribution to the high aggregation of PPI network or which are among proteins of independent functions are judged to be false positive connections. The experimental results on the dataset of saccharomyces cerevisiae PPI network show that the proposed algorithm has higher identification accuracy and matching rate when compared with the current representative identification algorithms of protein complexes. | WANG Xuesong CHENG Yuhu SUN Weifang | 2015 | Chinese Journal of Electronics2015，24，3： | 4 |
| 11 | Comparative transcriptome analyses shed light on carotenoid production and plastid development in melon fruit显示文摘Carotenoids,such asβ-carotene,accumulate in chromoplasts of various fleshy fruits,awarding them with colors,aromas,and nutrients.The Orange(CmOr)gene controlsβ-carotene accumulation in melon fruit by posttranslationally enhancing carotenogenesis and repressingβ-carotene turnover in chromoplasts.Carotenoid isomerase(CRTISO)isomerizes yellow prolycopene into red lycopene,a prerequisite for further metabolism intoβ-carotene.We comparatively analyzed the developing fruit transcriptomes of orange-colored melon and its two isogenic EMS-induced mutants,low-β(Cmor)and yofi(Cmcrtiso).The Cmor mutation in low-βcaused a major transcriptomic change in the mature fruit.In contrast,the Cmcrtiso mutation in yofi significantly changed the transcriptome only in early fruit developmental stages.These findings indicate that melon fruit transcriptome is primarily altered by changes in carotenoid metabolic flux and plastid conversion,but minimally by carotenoid composition in the ripe fruit.Clustering of the differentially expressed genes into functional groups revealed an association between fruit carotenoid metabolic flux with the maintenance of the photosynthetic apparatus in fruit chloroplasts.Moreover,large numbers of thylakoid localized photosynthetic genes were differentially expressed in low-β.CmOR family proteins were found to physically interact with light-harvesting chlorophyll a–b binding proteins,suggesting a new role of CmOR for chloroplast maintenance in melon fruit.This study brings more insights into the cellular and metabolic processes associated with fruit carotenoid accumulation in melon fruit and reveals a new maintenance mechanism of the photosynthetic apparatus for plastid development. | Noam Chayut Hui Yuan Yuval Saar Yi Zheng Tianhu Sun Xuesong Zhou Anna Hermanns Elad Oren Adi Faigenboim Maixia Hui Zhangjun Fei Michael Mazourek Joseph Burger Yaakov Tadmor Li Li | 2021 | Horticulture Research2021，8，1： | 3 |
| 12 | Generation and characterization of monoclonal antibodies against the NS1 protein of H17N10 bat influenza virus显示文摘 | Junna Gao Kaidi Xu Haiwei Sun Qinfang Liu Jianmei Yang Qiaoyang Teng Xuesong Li Zhaoguo Chen Zejun Li Hongjun Chen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 2 |
| 13 | Corrigendum to“Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration”[Bioact.Mater.23(2023)247-260]显示文摘This corrects the article“Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration”in volume 23 on page 247.The authors regret that the printed version of the above article contains some errors which are not identified during the proofing stage. | Junyuan Sun Fei Yang Lianlei Wang Haichao Yu Zhijie Yang Jingjing Wei Krasimir Vasilev Xuesong Zhang Xinyu Liu Yunpeng Zhao | 2023 | Bioactive Materials2023，，6： | 2 |
| 14 | Melatonin antagonizes ovarian aging via YTHDF2-MAPK-NF-κB pathway显示文摘Cellular senescence is closely associated with age-related diseases.Ovarian aging,a special type of organ senescence,is the pathophysiological foundation of the diseases of the reproductive system.It is characterized by the loss of integrity of the surface epithelium and a gradual decrease in the number of human ovarian surface epithelial cells(HOSEpiCs).To contribute to the research on delaying ovarian aging,we aimed to investigate the novel epigenetic mechanism of melatonin in protecting HOSEpiCs.We discovered that melatonin has antagonistic effects against the oncogene-induced senescence(OIS)of HOSEpiCs.Mechanistically,the oncogene Ras decreased the expression of YTHDF2,which is the reader of RNA-m6A,by stimulating the generation of reactive oxygen species(ROS).Moreover,we found that the suppression of YTHDF2 increased the expression of MAP2K4 and MAP4K4 by enhancing the stability of the transcription of their mRNAs,thereby upregulating the expression of the senescence-associated secretory phenotype(SASP)through the activation of the MAP2K4 and MAP4K4-dependent nuclear factor-κB(NF-κB)signaling pathways.We further determined that melatonin has antagonistic effects against the OIS of HOSEpiCs by inhibiting the ROS-YTHDF2-MAPK-NF-κB pathway.These findings provide key insights into the potential avenues for preventing and treating ovarian aging. | Ruigong Zhu Xian Ji Xuan Wu Jiajing Chen Xuesong Li Hong Jiang Haiping Fu Hui Wang Zhe Lin Xin Tang Shixiu Sun Qingguo Li Bingjian Wang Hongshan Chen | 2022 | Genes & Diseases2022，9，2： | 2 |
| 15 | Plant carotenoids: recent advances and future perspectives显示文摘Carotenoids are isoprenoid metabolites synthesized de novo in all photosynthetic organisms.Carotenoids are essential for plants with diverse functions in photosynthesis,photoprotection,pigmentation,phytohormone synthesis,and signaling.They are also critically important for humans as precursors of vitamin A synthesis and as dietary antioxidants.The vital roles of carotenoids to plants and humans have prompted significant progress toward our understanding of carotenoid metabolism and regulation.New regulators and novel roles of carotenoid metabolites are continuously revealed.This review focuses on current status of carotenoid metabolism and highlights recent advances in comprehension of the intrinsic and multi-dimensional regulation of carotenoid accumulation.We also discuss the functional evolution of carotenoids,the agricultural and horticultural application,and some key areas for future research. | Tianhu Sun Sombir Rao Xuesong Zhou Li Li | 2022 | Molecular Horticulture2022，2，1： | 2 |
| 16 | Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population显示文摘Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. | Liqun Zhou Kaiwei Yang Xuesong Li Yi Ding Dawei Mu Hanzhong Li Yong Yan Jinyi Li Dongwen Wang Wei Li Yulong Cong Jiangping Gao Kewei Ma Yajun Xiao Sheng Zhang Hongyi Jiang Weilie Hu Qiang Wei Xunbo Jin Zhichen Guan Qingyong Liu Danfeng Xu Xin Gao Yongguang Jiang Weimin Gan Guang Sun Qing Wang Yanhui Liu Jianquan Hou Liping Xie Xishuang Song Fengshuo Jin Jiafu Feng Ming Cai Zhaozhao Liang Jie Zhang Dingwei Ye Lin Qi Lulin Ma Jianzhong Shou Yuping Dai Jianyong Shao Ye Tian Shizhe Hong Tao Xu Chuize Kong Zefeng Kang Yuexin Liu Xun Qu Benkang Shi Shaobin Zheng Yi Lin Shujie Xia Dong Wei Jianbo Wu Weiling Fu Zhiping Wang Jianbo Liang | 2019 | Asian Journal of Urology2019，6，1： | 2 |
| 17 | A hybrid wavelet analysis and support vector machines in forecasting development of manufacturing显示文摘 | Guo Xuesong Sun Linyan Li Gang | 2008 | Expert Systems with Applications2008，35，12： | 1 |
| 18 | Effects of basic fibroblast growth factor on beta-catenin protein and mRNA expression during the proliferation of endogenous neural stem cells following focal cerebral ischemia显示文摘BACKGROUND:The Wnt/β-catenin signaling pathway plays an important role in neural development.β-catenin is an important component of the Wnt/β-catenin signaling pathway.The Wnt signaling pathway has been shown to regulate the interaction of neural stem cells with the extracellular matrix. OBJECTIVE:To investigate the effects of basic fibroblast growth factor(bFGF) onβ-catenin protein and mRNA expression,and on hippocampal neural stem cell proliferation in a rat model of cerebral ischemia/reperfusion. DESIGN,TIME AND SETTING:A randomized,controlled,neurobiology experiment was performed in Shenyang Medical College between August 2006 and August 2008. MATERIALS:A total of 72 healthy male Wistar rats,aged 3 months,were used in this study. bFGF was provided by Beijing SL Pharmaceutical Co.,Ltd.,China. METHODS:Rats were randomly divided into 3 groups:sham-operated,ischemia/reperfusion,and bFGF-treated(n=24 per group).Focal cerebral ischemia/reperfusion was induced in rats from the ischemia/reperfusion group and the bFGF-treated group by 2 hour right middle cerebral artery occlusion and 2 hour restoration of blood flow using the suture method.The ischemia/reperfusion and bFGF-treated groups were intraperitoneally administered 500 IU/mL of bFGF,or the same volume of physiological saline,once a day at postoperative days 1-3,and once every 3 days thereafter.Simultaneously,the sham-operated group underwent experimental procedures identical to the ischemia/reperfusion and bFGF-treated groups,with the exception of ischemia/reperfusion induction and drug administration.At 2 hours,2,6,13,and 20 days after ischemia/reperfusion induction,50 mg/kg bromodeoxyuridine(BrdU) was administered to each group,twice daily,to label proliferating neural stem cells. MAIN OUTCOME MEASURES:The effects of bFGF on BrdU labeling,andβ-catenin mRNA and protein expression,in neural stem cells were examined by immunohistochemistry,Western blot, RT-PCR,and in situ hybridization techniques. RESULTS:In the sham-operated group,only a few BrdU-immunoreactive neural stem cells were found.In the ischemia/reperfusion group,BrdU-immunoreactive cells began to increase from 3 days after ischemia/reperfusion induction,reached a peak level at 7 days,and gradually reduced from 21 days.At 3,7,14,and 21 days after ischemia/reperfusion induction,the numbers of BrdU-immunoreactive cells were significantly greater in the bFGF-treated group than in the ischemia/reperfusion group.The sham-operated group exhibited slight expression ofβ-catenin andβ-catenin mRNA.In the ischemia/reperfusion group,the expression ofβ-catenin andβ-catenin mRNA gradually increased with reperfusion time,peaked at 14 days after reperfusion, and gradually decreased thereafter;by 21 days,the expression was markedly lower.Following bFGF injection,the expression of hippocampal BrdU,β-catenin,andβ-catenin mRNA had apparently increased in each group. CONCLUSION:bFGF promotes neural stem cell proliferation,and the expression ofβ-catenin andβ-catenin mRNA in the ischemic brain tissue.These findings indicate that bFGF promotion of neural stem cell proliferation may be mediated by Wnt/β-catenin signaling pathway. | Xuesong Xing Weili Lü Liguang Sun | 2009 | Neural Regeneration Research2009，4，2： | 1 |
| 19 | The in vivo functions of a histidine-rich protein Hpn in Helicobacter pylori : Linking gastric and Alzheimer’s diseases together?显示文摘 | Ruiguang Ge Xuesong Sun | 2011 | Medical Hypotheses2011，，5： | 1 |
| 20 | Histidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells显示文摘 | Ruiguang Ge Xuesong Sun Dongxian Wang Qinglu Zhou Hongzhe Sun | 2011 | BBA - Molecular Cell Research2011，，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员