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| 1 | The calcium-dependent protein kinase ZmCDPK7 functions in heat-stress tolerance in maize显示文摘Global warming poses a serious threat to crops.Calcium-dependent protein kinases(CDPKs)/CPKs play vital roles in plant stress responses,but their exact roles in plant thermotolerance remains elusive.Here,we explored the roles of heat-induced ZmCDPK7 in thermotolerance in maize.ZmCDPK7-overexpressing maize plants displayed higher thermotolerance,photosynthetic rates,and antioxidant enzyme activity but lower H2 O2 and malondialdehyde(MDA)contents than wild-type plants under heat stress.ZmCDPK7-knockdown plants displayed the opposite patterns.ZmCDPK7 is attached to the plasma membrane but can translocate to the cytosol under heat stress.ZmCDPK7 interacts with the small heat shock protein sHSP17.4,phosphorylates sHSP17.4 at Ser-44 and the respiratory burst oxidase homolog RBOHB at Ser-99,and up regulates their expression.Site-directed mutagenesis of sHSP17.4 to generate a Ser-44-Ala substitution reduced ZmCDPK7’s enhancement of catalase activity but enhanced ZmCDPK7’s suppression of MDA accumulation in heat-stressed maize protoplasts.sHSP17.4,ZmCDPK7,and RBOHB were less strongly upregulated in response to heat stress in the abscisic acid-deficient mutant vp5 versus the wild type.Pretreatment with an RBOH inhibitor suppressed sHSP17.4 and ZmCDPK7 expression.Therefore,abscisic acid-induced ZmCDPK7 functions both upstream and downstream of RBOH and participates in thermotolerance in maize by mediating the phosphorylation of sHSP17.4,which might be essential for its chaperone function. | Yulong Zhao Hanwei Du Yankai Wang Huali Wang Shaoyu Yang Chaohai Li Ning Chen Hao Yang Yihao Zhang Yulin Zhu Luyao Yang Xiuli Hu | 2021 | Journal of Integrative Plant Biology2021，63，3： | 6 |
| 2 | Park7 interacts with p47phox to direct NADPH oxidase- dependent ROS production and protect against sepsis显示文摘 | Wenjun Liu Hailong Wu Lili Chen Yankai Wen Xiaoni Kong Wei-Qiang Gao | 2015 | Cell Research2015，25，6： | 5 |
| 3 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 4 | Population Genetics of SARS-CoV-2: Disentangling Effects of Sampling Bias and Infection Clusters显示文摘A novel RNA virus,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is responsible for the ongoing outbreak of coronavirus disease 2019(COVID-19).Population genetic analysis could be useful for investigating the origin and evolutionary dynamics of COVID-19.However,due to extensive sampling bias and existence of infection clusters during the epidemic spread,direct applications of existing approaches can lead to biased parameter estimations and data misinterpretation.In this study,we first present robust estimator for the time to the most recent common ancestor(TMRCA)and the mutation rate,and then apply the approach to analyze 12,909 genomic sequences of SARS-CoV-2.The mutation rate is inferred to be 8.69×10^(−4) per site per year with a 95%confidence interval(CI)of[8.61×10^(−4),8.77×10^(−4)],and the TMRCA of the samples inferred to be Nov 28,2019 with a 95%CI of[Oct 20,2019,Dec 9,2019].The results indicate that COVID-19 might originate earlier than and outside of Wuhan Seafood Market.We further demonstrate that genetic polymorphism patterns,including the enrichment of specific haplotypes and the temporal allele frequency trajectories generated from infection clusters,are similar to those caused by evolutionary forces such as natural selection.Our results show that population genetic methods need to be developed to efficiently detangle the effects of sampling bias and infection clusters to gain insights into the evolutionary mechanism of SARS-CoV-2.Software for implementing VirusMuT can be downloaded at http://gffzzdbc7b6aaae734bddsxvo9bvfkb5pp69no.ffgz.tsg.suse.edu.cn/biocode/tools/BT007081. | Qi Liu Shilei Zhao Cheng-Min Shi Shuhui Song Sihui Zhu Yankai Su Wenming Zhao Mingkun Li Yiming Bao Yongbiao Xue Hua Chen | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 1 |
| 5 | The fusion protein of HSP65 with tandem repeats of β-hCG acting as a potent tumor vaccine in suppressing hepatocarcinoma显示文摘 | Hu Xiangbing Zhang Yankai Lin Ming Lu Yong Zhang Yu Zhang Huiyong Chen Yingying Hou Jing Xing Yun Jin Liang Cao Rongyue Liu Jingjing | 2009 | International Immunopharmacology2009，，： | 1 |
| 6 | Multi-objective simulation-based evolutionary algorithm for an aircraft spare parts allocation problem显示文摘 | Loo Hay Lee Ek Peng Chew Suyan Teng Yankai Chen | 2007 | European Journal of Operational Research2007，，2： | 1 |
| 7 | Abnormal miR-214/A20 expression might play a role in T cell activation in patients with aplastic anemia显示文摘Aberrant T cell activation is a major cause of aplastic anemia(AA)pathogenesis.Recent studies have shown that miRNAs regulate T cell activation and are involved in AA.A previous study found that miR-214 was significantly up-regulated upon T cell activation in a CD28-dependent fashion by targeting PTEN.However,the expression characteristics of miR-214 and its target genes in AA have not been defined.In this study,target genes for miR-214 were predicted and confirmed by bioinformatics and luciferase reporter assays.The expression levels of miR-214 and target genes were detected in 36 healthy individuals and 35 patients with AA in peripheral blood mononuclear cells by real-time quantitative reverse transcriptase-polymerase chain reaction.Bioinformatics and luciferase reporter assays identified that miR-214 could bind to the A2030 untranslated regions.Significantly increased miR-214 and the decreased A20 expression level were detected in the AA patients compared with the healthy group.In addition,significantly increased miR-214 was found in non-severe aplastic anemia compared with severe aplastic anemia patients.These results suggested that the A20 gene was a potential target of miR-214,and elevated miR-214 might medicate T cell activation at least in part by regulating A20 expression in AA.We firstly confirmed that miR-214 regulated A20 expression,and aberrant miR-214/A20 expression might contribute to immunopathology in AA.The miR-214 expression might be used as a potential biomarker that assisted in diagnosing AA severity. | Zhi Yu Cunte Chen Yankai Xiao Xiaohui Chen Lixing Guo Guangxiao Tan Guixuan Huang Weifeng Luo Ming Zhou Yumiao Li Chen Lin Qi Shen Yuping Zhang Bo Li | 2020 | Blood Science2020，2，3： | 1 |
| 8 | Enantioselective direct aldol-type reaction of 4-hydroxycoumarin with ethyl trifluoropyruvate catalyzed by modified cinchona alkaloids显示文摘A new enantioselective aldol-type reaction of 4-hydroxycoumarins and ethyl trifluoropyruvate has been developed. In the presence of (DHQD)2PHAL (5 mol%), ethyl trifluoropyruvate could react with various 4-hydroxycoumarins to afford novel 4-hydroxycoumarin derivatives containing a tertiary trifluoromethyl alcohol center in good yields with moderate enantioselectivities. | CUI HaiLei JIANG Kun LIU YanKai Du Wei CHEN YingChun | 2010 | Chinese Science Bulletin2010，55，17： | 0 |
| 9 | Research on the Hotspots and Development of“Internet+Nursing Services”in China:A Bibliometric Analysis Based on CiteSpace显示文摘Objective:This study aims to explore the current research status,hotspots,and trends of the emerging nursing model of“Internet+nursing service”in China,and to provide theoretical references for further research and development.Methods:The literature related to“Internet+nursing service”was retrieved from the China National Knowledge Infrastructure(CNKI),Wanfang database,VIP database,and SinoMed.Subsequently,visual analysis was performed using the CiteSpace scientific knowledge mapping software.Results:A total of 1223 articles were included.The number of papers published in this field has been increasing yearly,and a core group of authors has been formed.Research institutions are mainly comprised of universities and hospitals and a high percentage of projects were funded provincially and municipally,while the least percentage of projects were funded nationally.The main research hotspots include the nurse standardized management for online appointments,the current application situation for the nursing model,and factors influencing its application amongst the elderly and also in women and children’s groups.Conclusion:Although the“Internet+nursing service”in China is still in its early stages,considerable progress has been made in relevant research.Future research should focus on identifying and omitting factors that hinder the industry’s growth,thereby establishing an effective long-term model of care. | Yankai Shi Shihua Cao Chen Zhu | 2024 | Journal of Clinical and Nursing Research2024，8，2： | 0 |
| 10 | A systematic review of the impacts of exposure to micro-and nano-plastics on human tissue accumulation and health显示文摘Micro-and nano-plastics(MNPs)pollution has become a pressing global environmental issue,with growing concerns regarding its impact on human health.However,evidence on the effects of MNPs on human health remains limited.This paper reviews the three routes of human exposure to MNPs,which include ingestion,inhalation,and dermal contact.It further discusses the potential routes of translocation of MNPs in human lungs,intestines,and skin,analyses the potential impact of MNPs on the homeostasis of human organ systems,and provides an outlook on future research priorities for MNPs in human health.There is growing evidence that MNPs are present in human tissues or fluids.Lab studies,including in vivo animal models and in vitro human-derived cell cultures,revealed that MNPs exposure could negatively affect human health.MNPs exposure could cause oxidative stress,cytotoxicity,disruption of internal barriers like the intestinal,the air–blood and the placental barrier,tissue damage,as well as immune homeostasis imbalance,endocrine disruption,and reproductive and developmental toxicity.Limitedly available epidemiological studies suggest that disorders like lung nodules,asthma,and blood thrombus might be caused or exacerbated by MNPs exposure.However,direct evidence for the effects of MNPs on human health is still scarce,and future research in this area is needed to provide quantitative support for assessing the risk of MNPs to human health. | Yudong Feng Chen Tu Ruijie Li Di Wu Jie Yang Yankai Xia Willie J.G.M.Peijnenburg Yongming Luo | 2023 | Eco-Environment & Health2023，2，4： | 0 |
| 11 | Optimization of Markov jump linear system with controlled modes jump probabilities显示文摘The optimal control of a Markov jump linear quadratic model with controlled jump probabilities of modes is investigated.Two kinds of mode control policies,i.e.,open-loop control policy and closed-loop control policy,are considered.Using the concepts of policy iteration and performance potential,the sufficient condition needed for the optimal closed-loop control policy to perform better than the optimal open-loop control policy is proposed.The condition is helpful for the design of an optimal controller.Furthermore,an efficient algorithm to construct a closed-loop control policy,which is better than the optimal open-loop control policy,is given with policy iteration. | Yankai XU Xi CHEN | 2009 | Frontiers of Electrical and Electronic Engineering in China2009，4，1： | 0 |
| 12 | Genetic diversity,population structure and core collection analysis of Hunan tea plant germplasm through genotyping-by-sequencing显示文摘Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant germplasm.In order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of Hunan tea plant germplasm through genotyping by sequencing(GBS)technology.As a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were obtained.Population structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three groups.The genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to 0.14.Analysis of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in Hunan tea germplasms.Furthermore,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire collection.This work should be valuable for conservation and utilization of tea germplasm in Hunan。 | Feiyi Huang Jihua Duan Yu Lei Zhen Liu Yankai Kang Yi Luo Yingyu Chen Youyong Li Shuoqian Liu Saijun Li Zhonghua Liu | 2022 | Beverage Plant Research2022，2，1： | 0 |
| 13 | Cumulative live birth rates of in vitro fertilization/intracytoplasmic sperm injection after multiple complete cycles in China显示文摘There were few studies of cumulative live birth rates(CLBRs) based on multicenter reproductive clinical data from the general Chinese population.Here we report a retrospective cohort study,including 14 311 women with17 315 cycles,in three reproductive centers to evaluate two estimated parameters of CLBRs with multiple transfer cycles of in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI) in a Chinese population.We found that CLBRs were related to female age and endometrial thickness.By the fourth transfer cycle,the conservative and optimal estimates of CLBRs were 52.95% and 77.30% in women under 30 years of age,and 18.17% and26.51% in those 37 years of age or older,respectively.The two estimates were 44.70% and 63.15% in women with endometrial thickness more than 7 mm,and 32.05% and 46.18% in those with less than 7 mm,respectively.In addition,body mass index(BMI),duration of infertility,and infertility diagnoses may also be related to CLBRs on certain conditions.The findings from this study on CLBRs after multiple transfer cycles of IVF/ICSI treatment on different conditions in the Chinese population should be beneficial to both infertile couples and clinicians. | Lei Huang Qun Lu Jiangbo Du Hong Lv Shiyao Tao Shiyao Chen Xiuzhu Li Xiumei Han Kun Zhou Bo Xu Xiaoyu Liu Hongxia Ma Yankai Xia Guangfu Jin Hongbing Shen Xiufeng Ling Zhibin Hu Jichun Tan Feiyang Diao | 2020 | The Journal of Biomedical Research2020，34，5： | 0 |
| 14 | Ongoing Positive Selection Drives the Evolution of SARS-CoV-2 Genomes显示文摘SARS-CoV-2 is a new RNA virus affecting humans and spreads extensively throughout the world since its first outbreak in December,2019.Whether the transmissibility and pathogenicity of SARS-CoV-2 in humans after zoonotic transfer are actively evolving,and driven by adaptation to the new host and environments is still under debate.Understanding the evolutionary mechanism underlying epidemiological and pathological characteristics of COVID-19 is essential for predicting the epidemic trend,and providing guidance for disease control and treatments.Interrogating novel strategies for identifying natural selection using within-species polymorphisms and 3,674,076 SARSCoV-2 genome sequences of 169 countries as of December 30,2021,we demonstrate with population genetic evidence that during the course of SARS-CoV-2 pandemic in humans,1)SARS-CoV-2 genomes are overall conserved under purifying selection,especially for the 14 genes related to viral RNA replication,transcription,and assembly;2)ongoing positive selection is actively driving the evolution of 6 genes(e.g.,S,ORF3a,and N)that play critical roles in molecular processes involving pathogen–host interactions,including viral invasion into and egress from host cells,and viral inhibition and evasion of host immune response,possibly leading to high transmissibility and mild symptom in SARS-CoV-2 evolution.According to an established haplotype phylogenetic relationship of 138 viral clusters,a spatial and temporal landscape of 556 critical mutations is constructed based on their divergence among viral haplotype clusters or repeatedly increase in frequency within at least 2 clusters,of which multiple mutations potentially conferring alterations in viral transmissibility,pathogenicity,and virulence of SARS-CoV-2 are highlighted,warranting attention. | Yali Hou Shilei Zhao Qi Liu Xiaolong Zhang Tong Sha Yankai Su Wenming Zhao Yiming Bao Yongbiao Xue Hua Chen | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 0 |
| 15 | On-chip ultrafast pulse generation based on graphene-silicon hybrid waveguides显示文摘On-chip ultrafast mode-locking lasers are basic building blocks for the realization of a chip-based optical frequency comb.In this paper,an ultrafast saturable absorber made up of a graphene pad on top of a silicon waveguide is applied to implement an ultrafast pulse laser.Benefiting from the small mode area of the graphene/silicon hybrid waveguide,the saturable pulse energy is reduced by two orders of magnitude compared with the fiber.A mode-locked pulse with a duration of 542 fs and a repetition rate of 54.37 MHz is realized.Pump–probe measurement shows that the carrier relaxation process of free carrier recombination with atomic-thin graphene/silicon junctions is three orders of magnitude faster than silicon,which plays a fundamental role in pulse narrowing.The chip-scale silicon ultrafast laser lays a foundation for a new class of nonlinear devices,in which a combination with multiple functional silicon photonic circuits enables efficient nonlinear interaction at the micrometer scale and less than 1 W of power consumption. | MENG DENG ZICHUN LIAO YANKAI CHEN NINGNING YANG XU YAN CHI ZHANG NENGLI DAI YI WANG | 2021 | Photonics Research2021，9，9： | 0 |
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