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| 1 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 2 | The non-canonical NF-κB pathway promotes NPC2 expression and regulates intracellular cholesterol trafficking显示文摘Niemann-Pick type C2(NPC2) is a lysosome luminal protein that functions in concert with NPC1 to mediate egress of lowdensity lipoprotein-derived cholesterol from lysosome. The nuclear factor kappa B subunit 2(NF-κB2) protein is a component of NF-κB transcription factor complex critically implicated in immune and inflammatory responses. Here, we report that NF-κB2 regulates intracellular cholesterol transport by controlling NPC2 expression. RNAi-mediated disruption of NF-κB2, as well as other signaling members of the non-canonical NF-κB pathway, caused intracellular cholesterol accumulation. Blockage of the non-canonical NF-κB pathway suppressed NPC2 expression, whereas Lymphotoxin β receptor(LTβR) activation or Baff receptor(BaffR) stimulation up-regulated the mRNA abundance and protein level of NPC2. Further, NF-κB2 activated NPC2 transcription through direct binding to its promoter region. We also observed cholesterol accumulation in NF-κB2-deficient zebrafish embryo and NF-κB2 mutant mice. Collectively, these data identify a regulatory role for the non-canonical NF-κB pathway in intracellular cholesterol trafficking and suggest a link between cholesterol transport and immune system. | Yacheng Liao Jian Wei Juqiong Wang Xiongjie Shi Jie Luo Bao-Liang Song | 2018 | Science China(Life Sciences)2018，61，10： | 4 |
| 3 | Generative urban design using shape grammar and block morphological analysis显示文摘The significant challenges that urban design faces when moving forward into this new digital era involve the increasingly massive and complex projects that must be analyzed and developed.The need for efficient analysis tools and rational design methods represent ongoing concerns,while practicable and maneuverable applications rather than experimental studies are scarce.This research implemented a framework of digital description and generative grammar of block form from the perspective of block morphological complexity.The implemented framework was tested in an urban design practice.First,this study adapted the hierarchical structure and access structure theory to analyze the spatial form of target blocks.The characteristics of blocks case samples in Nanjing are extracted as text descriptions.Second,the relevant composition patterns and parameters were employed as features for classificati on and were converted into procedural rules.With the top-down control by rules and bottom-up generation by shape grammar in CityEngine,the texture of the block can be generated as close to the actual block as possible.Furthermore,in a real urban design case located in Nanjing,this work applies the method to construct a three-dimensional scene quickly and accurately.After integrating design factors such as an environment,transportation,and vision and summarizing the intentions of blocks and buildings in corresponding functions and control indexes,the initial generation plan was built by applying the obtained characteristics and procedural rules in specific shape grammar.Finally,designers can adjust the result in detail by employing real-time calculation and interactive operation. | Xiao Wang Yacheng Song Peng Tang | 2020 | Frontiers of Architectural Research2020，9，4： | 4 |
| 4 | Multiple dimensions of urban design development from a practice perspective:A case study of an institute in Nanjing显示文摘The rapid urbanization process in China has promoted the active state of urban design practice.In the process of solving complex problems,the practical scope of urban design has gradually expanded.This paper took a design institution in Nanjing as a case study and sorted the trends in design project types and themes over the past 20 years,which presented a partial and diverse state of evolution in urban design practice.Concept,technology and management were extracted as the three main dimensions of cognitive urban design practice development.The relative positions and relationships of different types of urban design work were interpreted through mapping analysis of these three dimensions.This study provided a foundation for the practical orientation of urban design in China and a platform for communication a mong managers,urban design practitioners,scholars of related disciplines,and the public. | Dongqing Han Yacheng Song Enqi Wang Hua Liu Rong Fang | 2021 | Frontiers of Architectural Research2021，10，1： | 2 |
| 5 | Light from space illuminating the polar silk road显示文摘The rapid decline of Arctic sea ice has been reminding us the significant impacts caused by global warming.However,the other side of the coin is that this opens a window to utilize the Arctic sea routes in the summer seasons,bringing remarkable economic benefits for ocean transportation between Asia and Europe.However,commercial vessels with low ice classes must tackle substantial environmental challenges in the Arctic sea routes,particularly those caused by variable sea ice,even in the melting seasons.Therefore,the science-based support for shipping safety in the Arctic sea routes is being given more prominence.Emerging satellite remote sensing technology plays a critical role in environmental monitoring in the Arctic.This paper reviews state-of-the-art satellite observations on monitoring sea ice and potential applications on supporting shipping activities in the Arctic Ocean.Moreover,we introduced a recently developed system based on satellite observations to support the safe transportation of Chinese cargo vessels in the Arctic northern sea route,demonstrating the efforts by both the science and business communities to promote the development of the polar silk road. | Xiao-Ming Li Yujia Qiu Yacheng Wang Bingqing Huang Haiming Lu Min Chu Han Fu Fengming Hui | 2022 | International Journal of Digital Earth2022，15，1： | 0 |
| 6 | Lewis acid-oxygen vacancy interfacial synergistic catalysis over SO4^2-/Ce0.84Zr0.16O2-WO3-ZrO2 for N,N-diethylation of aniline with ethanol显示文摘Herein,a new mechanism involving Lewis acid-oxygen vacancy interfacial synergistic catalysis fo r aniline N,N-diethylation with ethanol was proposed,and the SO4^2-/Ce0.84Zr0.16O2-WO3-ZrO2 catalyst(SCWZ)with both Lewis acid sites and oxygen vacancies was synthesized by the hydrothermal method,which shows better catalytic activity than the reported solid acidic catalysts.Besides,the SO4^2-/ZrO2(SZ)and SO4^2-/WO3-ZrO2(SWZ)catalysts were also prepared and compared with SCWZ to investigate the synergistic effect of each component.The SO4^2-and WO3 mainly generate Lewis acid by bonding with ZrO2,which is beneficial for the fracture of the N-H bond in aniline.The Ce0.84Zr0.16O2 solid solution mainly plays a vital role in generating the oxygen vacancies as the interface active species,which can participate in stripping-OH from ethanol,then the carbocation will also be released,which only needs1.3805 kcal/mol energy,calculated by density functional theory(DFT),to be input.In comparison,the traditional reaction mechanism needs the Br■nsted acidic sites to promote the protonation of ethanol,then dehydration and subsequent formation of carbocation followed,and 108.6846 kcal/mol energy needs to be input,which is far higher than that of the new mechanism.The apparent activation energy(Ea)over SCWZ was measured by experiment to be 34.09 kJ/mol,which is much lower than that of SWZ(47.10 kJ/mol)and SZ(54.37 kJ/mol),illustrating comparatively preferable kinetics for SCWZ than that of SWZ and SZ.Besides,the conversion of aniline and selectivity to N,N-diethylaniline over SCWZ reach almost 100%and 73%,respectively,The SCWZ can be renewed for 4 times without rapid deactivation,and the longevity of SCWZ is longer than that of SWZ and SZ,as the loaded SO4^2-and tetragonal ZrO2 are stabilized by Ce0.84Zr0.16O2 and WO3,respectively. | Yacheng Liu Qifan Mao Xiaoyan Cao Xin Huang Kaijun Wang Can Wang Shuo Li Zhenggui Gu | 2020 | Journal of Rare Earths2020，38，11： | 0 |
| 7 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping ZhuZhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，1： | 0 |
| 8 | A Comprehensive Evaluation of User Satisfaction with Industrial Heritage Reuse and the Optimization Strategies:Taking Zhonglian U Valley 2.5 Creative Industrial Park as an Example显示文摘The industrial heritage creative industrial park in Qingdao,which has been renovated and put to use,was selected as the research subject.Based on the data collected via the questionnaire survey and fuzzy comprehensive evaluation,the hierarchical analysis method was used to calculate the weights of indicators at each level and construct a comprehensive satisfaction evaluation model,aiming to ensure a comprehensive satisfaction evaluation and analysis of the use effect of industrial heritage following renovation and utilization.Based on the evaluation results,the existing problems in the renovation and reuse of industrial heritage in Qingdao were analyzed,and subsequently corresponding optimization strategies were proposed. | Ran Wang Yacheng Guo Kang Qin Hailong Dong | 2022 | Journal of World Architecture2022，6，5： | 0 |
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