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| 1 | Identifying EGFR-Expressed Cells and Detecting EGFR Multi-Mutations at Single-Cell Level by Microfluidic Chip显示文摘EGFR mutations companion diagnostics have been proved to be crucial for the efficacy of tyrosine kinase inhibitor targeted cancer therapies. To uncover multiple mutations occurred in minority of EGFR-mutated cells,which may be covered by the noises from majority of unmutated cells, is currently becoming an urgent clinical requirement. Here we present the validation of a microfluidic-chip-based method for detecting EGFR multimutations at single-cell level. By trapping and immunofluorescently imaging single cells in specifically designed silicon microwells, the EGFR-expressed cellswere easily identified. By in situ lysing single cells, the cell lysates of EGFR-expressed cells were retrieved without cross-contamination. Benefited from excluding the noise from cells without EGFR expression, the simple and cost-effective Sanger's sequencing, but not the expensive deep sequencing of the whole cell population, was used to discover multi-mutations. We verified the new method with precisely discovering three most important EGFR drugrelated mutations from a sample in which EGFR-mutated cells only account for a small percentage of whole cell population. The microfluidic chip is capable of discovering not only the existence of specific EGFR multi-mutations,but also other valuable single-cell-level information: on which specific cells the mutations occurred, or whether different mutations coexist on the same cells. This microfluidic chip constitutes a promising method to promote simple and cost-effective Sanger's sequencing to be a routine test before performing targeted cancer therapy. | Ren Li Mingxing Zhou Jine Li Zihua Wang Weikai Zhang Chunyan Yue Yan Ma Hailin Peng Zewen Wei Zhiyuan Hu | 2018 | Nano-Micro Letters2018，10，1： | 2 |
| 2 | Bone/cartilage targeted hydrogel: Strategies and applications显示文摘The skeletal system is responsible for weight-bearing,organ protection,and movement.Bone diseases caused by trauma,infection,and aging can seriously affect a patient’s quality of life.Bone targeted biomaterials are suitable for the treatment of bone diseases.Biomaterials with bone-targeted properties can improve drug utilization and reduce side effects.A large number of bone-targeted micro-nano materials have been developed.However,only a few studies addressed bone-targeted hydrogel.The large size of hydrogel makes it difficult to achieve systematic targeting.However,local targeted hydrogel still has significant prospects.Molecules in bone/cartilage extracellular matrix and bone cells provide binding sites for bone-targeted hydrogel.Drug delivery systems featuring microgels with targeting properties is a key construction strategy for bone-targeted hydrogel.Besides,injectable hydrogel drug depot carrying bone-targeted drugs is another strategy.In this review,we summarize the bone-targeted hydrogel through application environment,construction strategies and disease applications.We hope this article will provide a reference for the development of bone-targeted hydrogels.We also hope this article could increase awareness of bone-targeted materials. | Hao Zhang Shunli Wu Weikai Chen Yan Hu Zhen Geng Jiacan Su | 2023 | Bioactive Materials2023，，5： | 2 |
| 3 | Two Ultraviolet Radiation Datasets that Cover China显示文摘Ultraviolet(UV) radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization's measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes. | Hui LIU Bo HU Yuesi WANG Guangren LIU Liqin TANG Dongsheng JI Yongfei BAI Weikai BAO Xin CHEN Yunming CHEN Weixin DING Xiaozeng HAN Fei HE Hui HUANG Zhenying HUANG Xinrong LI Yan LI Wenzhao LIU Luxiang LIN Zhu OUYANG Boqiang QIN Weijun SHEN Yanjun SHEN Hongxin SU Changchun SONG Bo SUN Song SUN Anzhi WANG Genxu WANG Huimin WANG Silong WANG Youshao WANG Wenxue WEI Ping XIE Zongqiang XIE Xiaoyuan YAN Fanjiang ZENG Fawei ZHANG Yangjian ZHANG Yiping ZHANG Chengyi ZHAO Wenzhi ZHAO Xueyong ZHAO Guoyi ZHOU Bo ZHU | 2017 | Advances in Atmospheric Sciences2017，34，7： | 2 |
| 4 | Cultivar evaluation and mega environment investigation based on the GGE biplot显示文摘 | Yan Weikai Hunt L A Sheng Qinglai | 2000 | Crop Science2000，40，3： | 1 |
| 5 | A heritability-adjusted GGE biplot for test environment evaluation显示文摘 | Weikai Yan James B. Holland | 2010 | Euphytica2010，，3： | 1 |
| 6 | An integrated biplot analysis system for displaying, interpreting and exploring genotype×environment interaction 显示文摘 | Yan Weikai Nicholas A T | 2005 | Crop Science2005，45，： | 1 |
| 7 | Associations among oat traits and their responses to the environment in North Ameriea显示文摘 | Yan Weikai Molnar S J Fregeau-Reid J | 2007 | J Crop Improve2007，20，： | 1 |
| 8 | A Heritability-adjusted GGE Bip- lot for test environment evaluation 显示文摘 | Yan Weikai Holland J B | 2010 | Euphytica2010，171，： | 1 |
| 9 | Non-structural carbohydrate levels of three co-occurring understory plants and their responses to forest thinning by gap creation in a dense pine plantation显示文摘We investigated non-structural carbohydrates(NSC) levels and components(starch,glucose,fructose and sucrose) in the leaves of three typical co-occurring forestfloor plants,moss Eurhynchium savatieri(ES),fern Parathelypteris nipponica(PN) and forb Aruncus sylvester(AS) in a 30-year-old Chinese pine(Pinus tabulaeformis)plantation forest on the eastern Tibetan Plateau.We also explored their responses to three gap creation treatments(control and two gap creations of 80 and 110 m2) based on NSC levels.PN had the highest leaf NSC level of the three plants,with AS second and ES lowest.Starch was the predominant component of NSC and the contents of glucose were higher than those of fructose or sucrose for all three species.The NSC level of ES in intermediate gaps was significantly higher than at control sites.PN also had higher NSC levels in both small and intermediate gaps than in control sites.But the differences between treatments were not obvious for AS.Our results suggest that ES and PN benefit from gap formation while the two species have different NSC response sensitivities to gap size,but the leaf NSC level of AS is less sensitive to the disturbance. | Zhe Wang Weikai Bao Xiaoli Yan | 2015 | Journal of Forestry Research2015，26，2： | 0 |
| 10 | Overview of the LAMOST survey in the first decade显示文摘The Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),also known as the Guoshoujing Telescope,is a major national scientific facility for astronomical research located in Xinglong,China.Beginning with a pilot survey in 2011,LAMOST has been surveying the night sky for more than 10 years.The LAMOST survey covers various objects in the Universe,from normal stars to peculiar ones. | Hongliang Yan Haining Li Song Wang Weikai Zong Haibo Yuan Maosheng Xiang Yang Huang Jiwei Xie Subo Dong Hailong Yuan Shaolan Bi Yaoquan Chu Xiangqun Cui Licai Deng Jianning Fu Zhanwen Han Jinliang Hou Guoping Li Chao Liu Jifeng Liu Xiaowei Liu Ali Luo Jianrong Shi Xuebing Wu Haotong Zhang Gang Zhao Yongheng Zhao | 2022 | The Innovation2022，3，2： | 0 |
| 11 | Similarity evaluation of stratum anti-drilling ability and a new method of drill bit selection显示文摘Considering the stratum anti-drilling ability,drill bit working conditions,drill bit application effect and drill bit economic benefits,the similarity of stratum anti-drilling ability was evaluated by grey relational analysis theory to screen out candidate drill bits with reference values.A new comprehensive performance evaluation model of drill bit was established by constructing the absolute ideal solution,changing the relative distance measurement method,and introducing entropy weight to work out the closeness between the candidate drill bits and ideal drill bits and select the reasonable drill bit.Through the construction of absolute ideal solution,improvement of relative distance measurement method and introduction of entropy weight,the inherent defects of TOPSIS decision analysis method,such as non-absolute order,reverse order and unreasonable weight setting,can be overcome.Simple in calculation and easy to understand,the new bit selection method has good adaptability to drill bit selection using dynamic change drill bit database.Field application has proved that the drill bits selected by the new drill bit selection method had significant increase in average rate of penetration,low wear rate,and good compatibility with the drilled formations in actual drilling.This new method of drill bit selection can be used as a technical means to select drill bits with high efficiency,long life and good economics in oilfields. | YAN Tie XU Rui SUN Wenfeng LIU Weikai HOU Zhaokai YUAN Yuan SHAO Yang | 2021 | Petroleum Exploration and Development2021，48，2： | 0 |
| 12 | Radial velocity measurements from LAMOST medium-resolution spectroscopic observations: a pointing towards the Kepler field显示文摘Radial velocity is one of the key measurements in understanding the fundamental properties of stars, stellar clusters and the Galaxy. A plate of stars in the Kepler field was observed in May of 2018 with the medium-resolution spectrographs of LAMOST, aiming to test the performance of this new system which is the upgraded equipment of LAMOST after the first five-year regular survey. We present our analysis on the radial velocity measurements(RVs) derived from these data. The results show that slight and significant systematic errors exist among the RVs obtained from the spectra collected by different spectrographs and exposures, respectively. After correcting the systematic errors with different techniques, the precision of RVs reaches ~1.3,~1.0,~0.5 and ~0.3 km s^(-1) at S/Nr = 10, 20, 50 and 100, respectively. Comparing with the RVs of standard stars from the APOGEE survey, our RVs are calibrated with a zero-point shift of~7 km s^(-1). The results indicate that the LAMOST medium-resolution spectroscopic system may provide RVs with a reasonable accuracy and precision for the selected targets. | Nian Liu Jian-Ning Fu Weikai Zong Jian-Rong Shi A-Li Luo Hao-Tong Zhang Xiang-Qun Cui Yong-Hui Hou Yang Pan Xin-Rui Shan Jian-Jun Chen Zhong-Rui Bai Jian-Xing Chen Bing Du Wen Hou Yu-Chen Liu Hao Tian Jiang-Tao Wang Jia-Xin Wang Ke-Fei Wu Yu-Zhong Wu Hong-Liang Yan Fang Zuo | 2019 | Research in Astronomy and Astrophysics2019，19，5： | 0 |
| 13 | Fabrication of Cu/graphite film/Cu sandwich composites with ultrahigh thermal conductivity for thermal management applications显示文摘Effective thermal management of electronic integrated devices with high powder density has become a serious issue,which requires materials with high thermal conductivity(TC).In order to solve the problem of weak bonding between graphite and Cu,a novel Cu/graphite film/Cu sandwich composite(Cu/GF/Cu composite)with ultrahigh TC was fabricated by electro-deposition.The micro-riveting structure was introduced to enhance the bonding strength between graphite film and deposited Cu layers by preparing a rectangular array of micro-holes on the graphite film before electrodeposition.TC and mechanical properties of the composites with different graphite volume fractions and current densities were investigated.The results showed that the TC enhancement generated by the micro-riveting structure for Cu/GF/Cu composites at low graphite content was more effective than that at high graphite content,and the strong texture orientation of deposited Cu resulted in high TC.Under the optimizing preparing condition,the highest in-plane TC reached 824.3 W·m^-1·K^-1,while the ultimate tensile strength of this composite was about four times higher than that of the graphite film. | Rui ZHAO Weikai L Tian WANG Ke ZHAN Zheng YANG Ya YAN Bin ZHAO Junhe YANG | 2020 | Frontiers of Materials Science2020，14，2： | 0 |
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