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| 1 | Homocysteine-mediated cholesterol efflux via ABCA1 and ACAT1 DNA methylation in THP-1 monocyte-derived foam cells显示文摘Homocysteine (Hcy ) 为心血管的事件作为一个流行风险因素被认出了。装载胆固醇的泡沫房间是动脉粥样硬化患者损害的一个中央部件。ATP 有约束力的盒子 transporter A1 (ABCA1 ) 它调停细胞的胆固醇和 phospholipids 的流出,是在类脂化合物的限制率的步新陈代谢。酰辅酶 A:cholesterol acyltransferase-1 (ACAT1 ) 在巨噬细胞支持胆固醇酉旨的累积,从而导致泡沫房间形成,在动脉粥样硬化的早舞台的一个特点。在这研究,有教养的导出单核白血球的泡沫房间为 24 h 与 Hcy 的临床的相关集中被孵化。胆固醇的房间和累积被发现的泡沫的两个都增加的数字,和 ABCA1 的 mRNA 和蛋白质表示层次被减少,当 ACAT1 表示面对 Hcy 被增加时。而且,而 ACAT1 DNA methylation 被使用 Hcy 的不同集中减少, ABCA1 基因的 DNA methylation 水平被增加。而且,我们的结果显示出那项 DNA methyltransferase (DNMT ) 活动和 DNA methyltransferase 1 (DNMT1 ) mRNA 表示被 Hcy 增加。DNA methylation 有功能经由 DNMT 调整 ABCA1 和 ACAT1 的表示,这被显示。在结论,这些结果建议 Hcy 导致的 ABCA1 和 ACAT1 DNA methylation 可以在导出单核白血球的泡沫房间在 ABCA1 和 ACAT1 表示和胆固醇的累积起一个潜在的作用。 | Yu Liang Xiaoling Yang Linna Ma Xin Cai Lei Wang Cheng Yang Guizhong Li Minghao Zhang Weiwei Sun Yideng Jiang | 2013 | Acta Biochimica et Biophysica Sinica2013，45，3： | 26 |
| 2 | Endoplasmic reticulum oxidoreductin 1α mediates hepatic endoplasmic reticulum stress in homocysteine-induced atherosclerosis显示文摘Endoplasmic 蜂窝胃(嗯) 应力作为不同病理学的过程的一个重要调节的人并且作为贡献导致的 homocysteine (Hcy ) 的机制正在出现 hepar 损害。然而,分子的事件导致 Hcy 嗯在在动脉粥样硬化背景下面的 hepar 的应力当前是未知的。Endoplasmic 蜂窝胃 oxidoreductin (ERO1 ) 1 在维持起一个关键作用嗯压力功能。在这研究,我们在 hepacytes 在 hyperhomocysteinemia 和 ERO1 的效果在 hepar 决定了 ERO1 的表示嗯应力面对 Hcy。HHcy 模型被在 apolipoprotein-E-deficient (ApoE-/-) 喂蛋氨酸食谱建立老鼠,和 hepatocytes 与 Hcy 的叶酸、不同的集中被孵化。我们的结果证明 Hcy 被触发嗯应力描绘了由一调整葡萄糖的蛋白质的增加的内容 78 (GRP78 ) ,蛋白质 kinase 象 RNA 一样嗯 kinase (津贴) ,激活抄写因素(ATF ) 6 并且 X 盒子有约束力的 protein-1 (XBP-1 ) 。在 HHcy 老鼠和对待 Hcy 的 hepatocytes 的 ERO1 表情在 ApoE-/- 组和控制 hepacytes 与那些相比被减少(P < 0.05 ) 分别地。有小介入的 RNA 的 ERO1 的表示显著地扩充了的敲门下面导致 Hcy 嗯应力。同时,表情嗯压力相关的因素包括 GRP78,津贴, ATF6 和 XBP-1,当 ERO1 基因在 hepacytes 是过去表示的时,显著地被减少。我们的结果建议 ERO1 可以涉及导致 Hcy 的 hepar 嗯 ERO1 表示的应力,和抑制能加速这进程。 | Xiaoling Yang Hua Xu Yinju Hao Li Zhao Xin Cai Jue Tian Minghao Zhang Xuebo Han Shengchao Ma Jun Cao Yideng Jiang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，10： | 14 |
| 3 | Degradation of bisphenol-A using ultrasonic irradiation assisted by low-concentration hydrogen peroxide显示文摘This study investigated the degradation of bisphenol-A (BPA) by ultrasonic irradiation in the presence of different additives (H2O2,air bubbles and humic acid) under various operating conditions,i.e.,ultrasonic frequency,power intensity and power density.The results demonstrated that the BPA degradation followed pseudo first-order kinetics under different experimental conditions.The optimum power intensities were 0.9,1.8,and 3.0W/cm 2 at the frequencies of 400,670,and 800 kHz,respectively.At the fixed frequency (800 kHz),the degradation rate of BPA was shown proportional to the increase of power density applied.With this manner,the BPA sonolysis could be facilitated at H2O2 dosage being lower than 0.1 mmol/L;while BPA degradation was hindered at H2O2 concentration in excess of 1 mmol/L.Additionally,BPA removal was shown to be inhibited by the presence of aeration and humic acid during ultrasonic irradiation.The present study suggested that the degradation rate of BPA assisted by ultrasonic irradiation was influenced by a variety of factors,and high BPA removal rate could be achieved under appropriate conditions. | Kejia Zhang Naiyun Gao Yang Deng Tsair Fuh Lin Yan Ma Lei Li Minghao Sui | 2011 | Journal of Environmental Sciences2011，23，1： | 9 |
| 4 | Scalable microfabrication of three-dimensional porous interconnected graphene scaffolds with carbon spheres for high-performance all carbon-based micro-supercapacitors显示文摘As one of the most important micro energy storage devices(MESDs),graphene-based micro-supercapacitors(G-MSCs)possess the advantages of excellent flexibility,long cycle life,affordability and high reliability.In most cases,constructing three-dimensional(3D)graphene networks is widely utilized to promote the permeation of electrolyte and enhance the utilization of active materials.In this work,conventional freeze-drying process is utilized in the fabrication of G-MSCs to constitute 3D interconnected networks micro-electrodes,and further by regulating the composition of inks,carbon spheres(CSs)at different mass loadings are introduced into the graphene scaffolds to further increase the active sites of the micro-electrodes.The fabricated all carbon-based MSC with the optimal mass loading of CSs(0.406 mg cm^(-2))exhibits a high specific areal capacitance of 17.01mF cm^(-2)at the scan rate of 10mV s^(-1)and a capacitance retention of 93.14%after 10000 cycles at the scan rate of 500 mV s^(-1).The proposed microfabrication process is facile and fully compatible with modern microtechnologies and will be highly suitable for large-scale production and integration. | Yiming Chen Minghao Guo Liang He Wei Yang Lin Xu Jiashen Meng Xiaocong Tian Xinyu Ma Qiang Yu Kaichun Yang Xufeng Hong Liqiang Mai | 2019 | Journal of Materiomics2019，5，2： | 6 |
| 5 | Large-scale blow spinning of heat-resistant nanofibrous air filters显示文摘Particulate matter(PM)pollution has become a serious problem worldwide and various kinds of nanofibrous filters aiming to solve the problem have been developed.It is urgent to remove PM from high-temperature pollution sources,such as industrial emissions,coal furnaces,and automobile exhaust gases.However,filtration at pollution sources remains challenging because most existing air filters are not resistant to high temperature.Herein,heat-resistant polyimide(PI)nanofibrous air filters are fabricated via a simple and scalable solution blow-spinning method.These air filters show excellent thermal stability at high temperature up to 420℃.They exhibit a filtration efficiency as high as 99.73%at ambient temperature and over 97%at 300℃.In addition,a field test shows that the filters remove>97%of PM from the car exhaust fumes.Hence,the blow-spun PI nanofibrous membranes combined with the facile preparation strategy have great potential in high temperature air filtration fields and other similar applications such as water purification and protein separation. | Ziwei Li Jianan Song Yuanzheng Long Chao Jia Zhenglian Liu Lei Li Cheng Yang Junchen Liu Sen Lin Haiyang Wang Yibo Liu Minghao Fang Hui Wu | 2020 | Nano Research2020，13，3： | 6 |
| 6 | Porous Si/C composite as anode materials for high-performance rechargeable lithium-ion battery显示文摘A novel porous silicon was synthesized through a magnesiothermic reduction method of molecular sieve for the first time, Si/C composite was synthesized by using pitch as carbon source. The porous Si/C composite shows a high initial specific capacity of 2018.5 mAh/g with current density of 0.1 A/g. When the current density increases to 2 A/g, it still exhibits high average specific capacity of 640.3 mAh/g. The porous structure can remit the Si particle pulverization during the lithiation/delithiation process. This article can provide a reference for the research of the porous Si anode for the high performance rechargeable lithium-ion battery. | Bingbing Deng Lian Shen Yangai Liu Tao Yang Manshu Zhang Renjie Liu Zhaohui Huang Minghao Fang Xiaowen Wu | 2017 | Chinese Chemical Letters2017，28，12： | 4 |
| 7 | Enhanced production of seed oil with improved fatty acid composition by overexpressing NAD^(+)-dependent glycerol-3-phosphate dehydrogenase in soybean显示文摘There is growing interest in expanding the production of soybean oils(mainly triacylglycerol, or TAG) to meet rising feed demand and address global energy concerns. We report that a plastidlocalized glycerol-3-phosphate dehydrogenase(GPDH), encoded by GmGPDHp1 gene, catalyzes the formation of glycerol-3-phosphate(G3 P), an obligate substrate required for TAG biosynthesis.Overexpression of GmGPDHp1 increases soybean seed oil content with high levels of unsaturated fatty acids(FAs), especially oleic acid(C18:1), without detectably affecting growth or seed protein content or seed weight. Based on the lipidomic analyses, we found that the increase in G3 P content led to an elevated diacylglycerol(DAG) pool, in which the Kennedy pathwayderived DAG was mostly increased, followed by PC-derived DAG, thereby promoting the synthesis of TAG containing relatively high proportion of C18:1. The increased G3 P levels induced several transcriptional alterations of genes involved in the glycerolipid pathways. In particular, genes encoding the enzymes responsible for de novo glycerolipid synthesis were largely upregulated in the transgenic lines, in-line with the identified biochemical phenotype. These results reveal a key role for GmGPDHp1-mediated G3 P metabolism in enhancing TAG synthesis and demonstrate a strategy to modify the FA compositions of soybean oils for improved nutrition and biofuel. | Ying Zhao Pan Cao Yifan Cui Dongxu Liu Jiapeng Li Yabin Zhao Siqi Yang Bo Zhang Runnan Zhou Minghao Sun Xuetian Guo Mingliang Yang Dawei Xin Zhanguo Zhang Xin Li Chen Lv Chunyan Liu Zhaoming Qi Jingyu Xu Xiaoxia Wu Qingshan Chen | 2021 | Journal of Integrative Plant Biology2021，63，6： | 4 |
| 8 | Photosynthetic acclimation to long-term high temperature and soil drought stress in two spruce species (Picea crassifolia and P. wilsonii) used for afforestation显示文摘Picea crassifolia and P.wilsonii,commonly used for afforestation in northern China,are increasingly likely to be subjected to high temperatures and soil drought stress as a result of global warming.However,little is known about the effects of these stresses on foliar photosynthesis in the two species.To investigate how photosynthetic characteristics and sensitivity respond to prolonged high temperatures and soil drought,foliar gas exchange and other closely related parameters were recorded from four-year-old seedlings of both species.Seedlings were grown under two temperature treatments(25/15 and 35/25 °C) and four soil water regimes [80,60,40 and 20% of maximum field capacity(FC)] for 4 months.Although all treatments significantly reduced photosynthetic rates(P_n) of both species,P.crassifolia exhibited greater photosynthetic acclimation than P.wilsonii.Differences in photosynthetic acclimation were mainly related to variations in stomatal conductance(Cond) and the maximum quantum yield of PSII(F_v/F_m) between treatments.Indeed,higher Cond and F_v/F_m in all treatments were shown for P.crassifolia than for P.wilsonii.Moreover,photosynthesis in P.crassifolia exhibited inherently lower temperature sensitivities(broader span for the temperature response curves; lower b) and higher thermostability(invariable b between treatments).Further,severe drought stress(20% FC) limited the survival of P.wilsonii.Our results indicate that P.wilsonii is more susceptible to high temperatures and soil drought stress.Planting P.crassifolia would be more expected to survive these conditions and hence be of greater benefit to forest stability if predicted increases in drought and temperature in northern China occur. | Xiaowei Zhang Litong Chen Jingru Wang Minghao Wang Shuli Yang Changming Zhao | 2018 | Journal of Forestry Research2018，29，2： | 4 |
| 9 | Flexible polyimide-based hybrid optoelectric neural interface with 16 channels of micro-LEDs and electrodes显示文摘In this paper,a polyimide-based flexible device that integrates 16 micro-LEDs and 16 IrO_(x)-modified microelectrodes for synchronous photostimulation and neural signal recording is presented.The 4×4 micro-LEDs(dimensions of 220×270×50μm^(3),700μm pitch)are fixed in the SU-8 fence structure on a polyimide substrate and connected to the leads via a wire-bonding method.The recording electrodes share a similar fabrication process on the polyimide with 16 microelectrode sites(200μm in diameter and 700μm in pitch)modified by iridium oxide(IrO_(x)).These two subparts can be aligned with alignment holes and glued back-to-back by epoxy,which ensures that the light from the LEDs passes through the corresponding holes that are evenly distributed around the recording sites.The long-term electrical and optical stabilities of the device are verified using a soaking test for 3 months,and the thermal property is specifically studied with different duty cycles,voltages,and frequencies.Additionally,the electrochemical results prove the reliability of the IrO_(x)-modified microelectrodes after repeated pressing or friction.To evaluate the tradeoff between flexibility and strength,two microelectrode arrays with thicknesses of 5 and 10μm are evaluated through simulation and experiment.The proposed device can be a useful mapping optogenetics tool for neuroscience studies in small(rats and mice)and large animal subjects and ultimately in nonhuman primates. | Bowen Ji Zhejun Guo Minghao Wang Bin Yang Xiaolin Wang Wen Li Jingquan Liu | 2018 | Microsystems & Nanoengineering2018，4，1： | 3 |
| 10 | Effects of Angular Fillers on Thermal Runaway of Lithium-Ion Battery显示文摘By adding 1 wt% damage homogenizer(DH), i.e. carbon black microparticles, into the electrodes of lithiumion batteries, thermal runaway can be mitigated as the battery cells are subjected to impact loadings. In a drop tower test, the generated heat of the modified cells is reduced by nearly 40%, compared with the reference cells. This phenomenon may be attributed to the weakening effect of the carbon black fillers.The shape of the filler grains does not have a pronounced influence on the temperature profile. | Meng Wang Anh V.Le Yang Shi Daniel J.Noelle Hyojung Yoon Minghao Zhang Y.Shirley Meng Yu Qiao | 2016 | Journal of Materials Science & Technology2016，32，11： | 3 |
| 11 | Important population viability analysis parameters for giant pandas(Aliuropoda melanoleuca)显示文摘Population viability analysis(PVA) is a tool to evaluate the risk of extinction for endangered species and aid conservation decision-making.The quality of PVA output is dependent on parameters related to population dynamics and life-history;however,it has been difficult to collect this information for the giant panda(Aliuropoda melanoleuca),a rare and endangered mammal native to China,confined to some 30 fragmented habitat patches.Since giant pandas are long-lived,mature late,have lower reproductive rates,and show little sexual dimorphism,obtaining data to perform adequate PVA has been difficult.Here,we develop a parameter sensitivity index by modeling the dynamics of six giant panda populations in the Minshan Mountains,in order to determine the parameters most influential to giant panda populations.Our data shows that the giant panda populations are most sensitive to changes in four female parameters:initial breeding age,reproductive rate,mortality rate between age 0 and 1,and mortality rate of adults.The parameter sensitivity index strongly correlated with initial population size,as smaller populations were more sensitive to changes in these four variables.This model suggests that demographic parameters of females have more influence on the results of PVA,indicating that females may play a more important role in giant panda population dynamics than males.Consequently,reintroduction of female individuals to a small giant panda population should be a high priority for conservation efforts.Our findings form a technical basis for the coming program of giant panda reintroduction,and inform which parameters are crucial to successfully and feasibly monitoring wild giant panda populations. | Minghao GONG Yanling SONG Zhisong YANG Chen LIN | 2012 | Zoological Research2012，33，3： | 3 |
| 12 | Different Impacts of Madden–Julian Oscillation on Winter Rainfall over Southern China显示文摘Winter rainfall over southern China is usually enhanced when Madden–Julian oscillation(MJO) is active over the Indian Ocean, but it can be weakened under certain conditions. Here, the diversity of MJO impacts on winter rainfall and its mechanisms are explored by using scenarios of enhanced and suppressed rainfall anomalies over southern China when MJO is active over the Indian Ocean. The combined effects of low-frequency background moisture and intraseasonal winds are the major contributors to the different rainfall anomalies. Anomalous circulation in mid–high latitudes, especially on intraseasonal timescales, is almost opposite in the two scenarios, which can modulate the response of extratropical atmosphere to MJO heating and then induces the different circulations over southern China. In the enhanced scenario, mid–high latitudes of Eurasia and southern China are dominated by positive and negative sea level pressure anomalies, respectively. The southerly over southern China and the South China Sea induced by MJO heating promotes the anomalous moisture convergence and ascending motion over southern China, resulting in the enhanced rainfall. In the suppressed scenario, however, the circulation in mid–high latitudes does not favor rainfall over southern China and leads to the northerly response to MJO heating over southern China, which enhances moisture divergence and weakens rainfall over southern China. | Xiong CHEN Jian LING Chongyin LI Lifeng LI Minghao YANG | 2021 | Journal of Meteorological Research2021，35，2： | 2 |
| 13 | Individual aortic baroreceptors are sensitive to different ranges of blood pressures显示文摘Many receptors,including thermal receptors and mechanical receptors,are only activated by stimuli within a clearly defined range of intensities.Differences in the receptive ranges enable individual receptors and their sensory centers to precisely detect the intensity of the stimulus and changes in intensity.Baroreceptors are the sensory terminals of the baroreflex.It is well understood that an increasing number of baroreceptors are recruited to produce afferent action potentials as the blood pressure increases,indicating that individual baroreceptors have different pressure thresholds.The present study revealed that individual baroreceptors could stop their afferent signals when the blood pressure exceeds a certain level,indicating that individual baroreceptors are sensitive to a specific range of blood pressure.The receptive ranges of individual baroreceptors differ in terms of the total range,the lower threshold,and the upper threshold.Of 85 baroreceptors examined in this study,the upper thresholds for about half were within the physiological blood pressure range.These results indicate that supraphysiological blood pressure is unlikely to be encoded by the recruitment of more baroreceptors.Instead,supraphysiological blood pressure levels might be signaled by an increase in the frequency of action potentials or by other mechanisms.In conclusion,our results indicate that rabbit baroreceptors are activated by blood pressure levels within specific receptive ranges.These findings should encourage further studies to examine the role of population coding of blood pressure by baroreceptors in the baroreflex. | CHEN MengJiao YANG MingHao HAN WenJuan AN ShuCheng LIU YiHui LIU ZhiQiang REN Wei | 2014 | Science China(Life Sciences)2014，57，5： | 2 |
| 14 | Multi-objective optimization of operation loop recommendation for kill web显示文摘In order to improve our military ’s level of intelligent accusation decision-making in future intelligent joint warfare, this paper studies operation loop recommendation methods for kill web based on the fundamental combat form of the future, i.e.,“web-based kill,” and the operation loop theory. Firstly, we pioneer the operation loop recommendation problem with operation ring quality as the objective and closed-loop time as the constraint, and construct the corresponding planning model.Secondly, considering the case where there are multiple decision objectives for the combat ring recommendation problem,we propose for the first time a multi-objective optimization algorithm, the multi-objective ant colony evolutionary algorithm based on decomposition(MOACEA/D), which integrates the multi-objective evolutionary algorithm based on decomposition(MOEA/D) with the ant colony algorithm. The MOACEA/D can converge the optimal solutions of multiple single objectives nondominated solution set for the multi-objective problem. Finally,compared with other classical multi-objective optimization algorithms, the MOACEA/D is superior to other algorithms superior in terms of the hyper volume(HV), which verifies the effectiveness of the method and greatly improves the quality and efficiency of commanders’ decision-making. | YANG Kewei XIA Boyuan CHEN Gang YANG Zhiwei LI Minghao | 2022 | Journal of Systems Engineering and Electronics2022，33，4： | 1 |
| 15 | Longwall mining under gateroads and gobs of abandoned small mine显示文摘Due to the use of outdated mining technology or room and pillar mining process in small coal mines, the coal recovery ratio is only 10–25%. In many regions of China, the damage area caused by the small coal mines amounted to nearly one hundred square kilometers. Therefore, special mining techniques must be taken to reclaim the wasted resource in disturbed coal areas. This paper focuses on the different mining methods by analyzing the longwall panel layout and abandoned gateroad(AG) distribution in the abandoned area of Cuijiazhai coal mine in northwestern China. On the basis of three-dimensional geological model, FLAC3 D numerical simulation was employed. The abutment pressure distribution was simulated when the panel face passed through the disturbed areas. The proper angle of the inclined face was analyzed when the panel face passed through the abandoned gateroads. The results show that the head end of the face should be 13–20 m ahead of the tail end. The pillars on both sides of abandoned gateroads had not been damaged at the same time, and no large-area stress concentration occured above the main roof.Therefore, the coal reserves of disturbed areas can be successfully recovered by using underground longwall mining. | Li Yang Zhu Enguang Zhang Kangning Li Minghao Wang Jiaxing Li Chengkun | 2017 | International Journal of Mining Science and Technology2017，27，1： | 1 |
| 16 | MHC-mismatched mice liver transplantation promotes tumor growth in liver graft显示文摘 | Sheng Yan Yuan Ding Yang Tian Zhongjie Lu Yan Wang Qiyi Zhang Yufu Ye Lin Zhou Haiyang Xie Hui Chen Minghao Zheng Shusen Zheng | 2014 | Cancer Letters2014，，： | 1 |
| 17 | Review of micro-expression spotting and recognition in video sequences显示文摘Facial micro-expressions are short and imperceptible expressions that involuntarily reveal the true emotions that a person may be attempting to suppress,hide,disguise,or conceal.Such expressions can reflect a person's real emotions and have a wide range of application in public safety and clinical diagnosis.The analysis of facial micro-expressions in video sequences through computer vision is still relatively recent.In this research,a comprehensive review on the topic of spotting and recognition used in micro expression analysis databases and methods,is conducted,and advanced technologies in this area are summarized.In addition,we discuss challenges that remain unresolved alongside future work to be completed in the field of micro-expression analysis. | Hang PAN Lun XIE Zhiliang WANG Bin LIU Minghao YANG Jianhua TAO | 2021 | Virtual Reality & Intelligent Hardware2021，3，1： | 1 |
| 18 | Water Vapor Transport Related to the Interdecadal Shift of Summer Precipitation over Northern East Asia in the Late 1990s显示文摘In this study, an interdecadal shift of summer precipitation over northern East Asia(NEA) was identified, demonstrating that summer precipitation decreased abruptly after 1998/99. The synchronous shift in summer moisture budget and water vapor transport over NEA was further investigated by using the NCEP/NCAR reanalysis data. The results indicate that water vapor transported northward into NEA from three low-latitude paths was limited because most water vapor was transported eastward. Water vapor transported from the westerly path in mid–high(WMH) latitudes exhibited significant correlations with summer precipitation in NEA and experienced a significant adjustment in the late 1990s. Regarding the spatial distributions of water vapor transport, less input was found through the western boundary while more output occurred through the eastern boundary of NEA, and zonal water vapor transport fluxes mainly concentrated at the low to middle levels, which led to the summer precipitation shift in NEA around the late 1990s. Furthermore, it is also confirmed that the wind anomalies(rather than the moisture disturbance) as the dominant internal dynamic factor and Pacific Decadal Oscillation/Atlantic Multidecadal Oscillation(PDO/AMO) as possible external force played important roles in influencing the water vapor transport and causing the summer precipitation shift over NEA in the late 1990s. | Po HU Minghao WANG Liu YANG Xiaojuan WANG Guolin FENG | 2018 | Journal of Meteorological Research2018，32，5： | 1 |
| 19 | Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes显示文摘Gas vesicles are hollow, air-filled polyprotein structures that provide the buoyancy to cells. They are found in a variety of prokaryotes. In this study, we isolated a partial gas vesicle protein gene cluster containing gvp A and gvp C20Ψ from Planktothrix rubescens, and inserted it into an expression vector and expressed it in E. coli. The gas vesicle was developed in bacterial cells, which made bacterial cells to float on medium surface. We also amplified gvp A and gvp C20Ψ separately and synthesized an artificial operon by fusing these two genes with the standardized gene expression controlling elements of E. coli. The artificial operon was expressed in E. coli, forming gas vesicles and floating bacteria cells. Our findings verified that the whole set of genes and the overall structure of gas vesicle gene cluster are not necessary for developing gas vesicles in bacteria cells. Two genes, gvp A and gvp C20Ψ, of the gas vesicle gene cluster are sufficient for synthesizing an artificial operon that can develop gas vesicles in bacteria cells. Our findings provided a wide range of applications including easing the harvest of cultured microalgae and bacteria, as well as enriching and remediating aquatic pollutants by constructing gas vesicles in their cells. | WANG Tianhe KANG Li LI Jiaheng WU Wenjie ZHANG Peiran GONG Minghao LAI Weihong ZHANG Chunyan CHANG Lei PENG Yong YANG Zhongzhou LI Lian BAO Yingying XU Haowen ZHANG Xiaohua SUI Zhenghong YANG Guanpin WANG Xianghong | 2015 | Journal of Ocean University of China2015，14，1： | 1 |
| 20 | Gluconic Acid Aqueous Solution as a Sustainable and Reeyclable Promoting Medium for Organic Reaetions显示文摘 | ZHOU Binghua YANG Jie LI Minghao | 2011 | Green Chem2011，13，： | 1 |
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