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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 3 | Landscape of emerging and re-emerging infectious diseases in China: impact of ecology, climate, and behavior显示文摘为过去的几十年,在中国的传染疾病侧面在社会、经济的方面,环境,食物的质量,水,住房,和公共健康基础结构与快速的开发一直在变。尤其是, 5 应具报的传染疾病几乎被根除了,并且 18 另外的应具报的传染疾病的发生显著地被减少了。出人意料地,包括 HIV,普鲁斯病,梅毒,和登革热发烧,超过 10 应具报的传染疾病的发生一直在增加。不过,经常的传染疾病爆发 / 事件每年几乎被报导了,并且进口了传染疾病自从 2015,增加了。已知的病原体的新病原体和超过 100 新遗传型或 serotypes 被识别了。一些传染疾病似乎被各种各样的因素加重,包括快速的都市化,大量候鸟工人,在气候的变化,生态学,和政策,例如把农田还给森林。这评论在设法出现和 re-emerging 从中国总结当前的经验和课传染疾病,特别生态学的效果,气候,和行为,它应该在帮助另外的国家控制并且阻止传染疾病有优点。 | Qiyong Liu Wenbo Xu Shan Lu Jiafu Jiang Jieping Zhou Zhujun Shao Xiaobo Liu Lei Xu Yanwen Xiong Han Zheng Sun Jin Hai Jiang Wuchun Cao Jianguo Xu | 2018 | Frontiers of Medicine2018，12，1： | 23 |
| 4 | Human mesenchymal stromal cells enhance the immunomodulatory function of CD8+CD28- regulatory T cells显示文摘 | Qiuli Liu Haiqing Zheng Xiaoyong Chen Yanwen Peng Weijun Huang Xiaobo Li Gang Li Wenjie Xia Qiquan Sun an Andy Peng Xiang | 2015 | Cellular & Molecular Immunology2015，12，6： | 12 |
| 5 | PREPARATION OF Al-Sc ALLOY IN CHLORIDE SYSTEM WITH MOLTEN SALT ELECTROLYSIS显示文摘INTRODUCTIONScandiumposesesexcelentphysicochemicalproperties.WhenalitleofScisaddedintoAlaloy,thestrengthandhardnesofAlaloyca... | Tian Yanwen, Sun Benliang and Zhai Yuchun School of Materials & Metallurgy, Northeastern University, Shenyang 110006, P.R.China | 1998 | 中国有色金属学会会刊:英文版1998，8，4： | 3 |
| 6 | Fabrication of Periodic Nanostructures Using AFM Tip-Based Nanomachining:Combining Groove and Material Pile-Up Topographies显示文摘This paper presents an atomic force microscopy(AFM)tip-based nanomachining method to fabricate periodic nanostructures.This method relies on combining the topography generated by machined grooves with the topography resulting from accumulated pile-up material on the side of these grooves.It is shown that controlling the distance between adjacent and parallel grooves is the key factor in ensuring the quality of the resulting nanostructures.The presented experimental data show that periodic patterns with good quality can be achieved when the feed value between adjacent scratching paths is equal to the width between the two peaks of material pile-up on the sides of a single groove.The quality of the periodicity of the obtained nanostructures is evaluated by applying one-and two-dimensional fast Fourier transform(FFT)algorithms.The ratio of the area of the peak part to the total area in the normalized amplitude–frequency characteristic diagram of the cross-section of the measured AFM image is employed to quantitatively analyze the periodic nanostructures.Finally,the optical effect induced by the use of machined periodic nanostructures for surface colorization is investigated for potential applications in the fields of anti-counterfeiting and metal sensing. | Yanquan Geng Yongda Yan Jiqiang Wang Emmanuel Brousseau Yanwen Sun Yazhou Sun | 2018 | Engineering2018，4，6： | 3 |
| 7 | Energy measurement system of a large-aperture high power laser experiment platform显示文摘An energy measurement system in a large-aperture high power laser experiment platform is introduced. The entire measurement system includes five calorimeters, which carry out the energy measurement of the fundamental frequency before the frequency conversion unit, remaining fundamental frequency, remaining second-harmonics, third-harmonics,as well as the energy balance measurement after the frequency conversion unit. Combinational indirect calibration and direct calibration are employed to calibrate the sampling coefficients of the calorimeters. The analysis of the data showed that, regarding the energy balance coefficients, combinational calibration approach gives a higher precision, and leads to an energy balance with 1%; and regarding the energy sampling coefficients for the various wavelengths after the frequency conversion, the results from direct and combinational calibration are consistent. The uncertainties for all energy sampling coefficients are within 3%, which guarantees the reliability of the energy measurement for the laser facility. | Yanwen Xia Yue Liang Sen Li Junpu Zhao Zhitao Peng Hongguang Li Hua Liu Zhihong Sun Kuixing Zheng Xiaofeng Wei | 2013 | High Power Laser Science and Engineering2013，1，Z1： | 2 |
| 8 | Long non-coding RNAs: A new frontier in the study of human diseases显示文摘 | Xuefei Shi Ming Sun Hongbing Liu Yanwen Yao Yong Song | 2013 | Cancer Letters2013，，： | 2 |
| 9 | PM_(2.5) exposure associated with microbiota gut-brain axis:Multi-omics mechanistic implications from the BAPE study显示文摘Recent studies have shown that PM_(2.5) may activate the hypothalamus-pituitary-adrenal(HPA)axis by inducing hormonal changes,potentially explaining the increase in neurological and cardiovascular risks.In addition,an association between PM_(2.5) and gut microbiota and metabolites was established.The above evidence represents crucial parts of the gut-brain axis(GBA).In view of this evidence. | Tiantian Li Jianlong Fang Song Tang Hang Du Liang Zhao Yanwen Wang Fuchang Deng Yuanyuan Liu Yanjun Du Liangliang Cui Wanying Shi Yan Wang Jiaonan Wang Yingjian Zhang Xiaoyan Dong Ying Gao Yu Shen Li Dong Huichan Zhou Qinghua Sun Haoran Dong Xiumiao Peng Yi Zhang Meng Cao Hong Zhi Jingyang Zhou Xiaoming Shi | 2022 | The Innovation2022，3，2： | 2 |
| 10 | Fourier tranform mid - infrared (MIR) and near- infrared (NIR) spectroscopy for raid quality assessment of Chinese medicine preparation Honghua Oil显示文摘 | Wu Yanwen Sun Suqin Zhou Qun | 2008 | Journal of Pharmaceutical and Biomedical Analysis2008，46，： | 1 |
| 11 | First-principles calcu-lations of the structural,electronic,and optical properties ofLiF up to 300 GPa显示文摘 | Sun Zhihong Dong Jun Xia Yanwen | 2011 | Physica B2011，406，19： | 1 |
| 12 | Long non-coding RNAs: A new frontier in the study of human diseases显示文摘 | Xuefei Shi Ming Sun Hongbing Liu Yanwen Yao Yong Song | 2013 | Cancer Letters2013，，： | 1 |
| 13 | Chronological Constraints on Late Paleozoic Collision in the Southwest Tianshan Orogenic Belt, China: Evidence from the Baleigong Granites显示文摘The Baleigong granites, located in the western part of the southwestern Tianshan Orogen(Kokshanyan region, China), records late Paleozoic magmatism during the late stages of convergence between the Tarim Block and the Central Tianshan Arc Terrane. We performed a detailed geochronological and geochemical study of the Baleigong granites to better constrain the nature of collisional processes in the Southwest Tianshan Orogen. The LA-ICP-MS U-Pb zircon isotopic analyses indicate that magmatism commenced in the early Permian(~282 Ma). The granite samples, which are characterized by high contents of SiO2(67.68-69.77 wt%) and Al2O3(13.93-14.76 wt%), are alkali-rich and Mg-poor, corresponding to the high-K calc-alkaline series. The aluminum saturation index(A/CNK) ranges from 0.93 to 1.02, indicating a metaluminous to slightly peraluminous composition. Trace element geochemistry shows depletions in Nb, Ta, and Ti, a moderately negative Eu anomaly(δEu=0.40-0.56), enrichment in LREE, and depletion in HREE((La/Yb)N=7.46-11.78). These geochemical signatures are characteristic of an I-type granite generated from partial melting of a magmatic arc. The I-type nature of the Baleigong granites is also supported by the main mafic minerals being Fe-rich calcic hornblende and biotite. We suggest that the high-K, calc-alkaline I-type granitic magmatism was generated by partial melting of the continental crust, possibly triggered by underplating by basaltic magma. These conditions were likely achieved in a collisional tectonic setting, thus supporting the suggestion that closure of the South Tianshan Ocean was completed prior to the Permian and was followed(in the late Paleozoic) by collision between the Tarim Block and the Central Tianshan Arc Terrane. | HUO Hailong CHEN Zhengle ZHANG Qing HAN Fengbin ZHANG Wengao SUN Yue YANG Bin TANG Yanwen | 2019 | Acta Geologica Sinica(English Edition)2019，93，5： | 1 |
| 14 | Measurement on Physicochemical Properties ofNaCl-KCl-ScCl_3 System for Manufacture of Al-Sc Alloy by Molten Salt Electrolysis显示文摘The physicochemical properties of the system, such as density, surface tension, specific conductance and melting point were measured. The results were discussed. | Yanwen TIAN Benliang SUN and Yuchun ZHAI(Materials and Metallurgy School, Northeastern University, Shenyang 110006, China) | 1999 | Journal of Materials Science & Technology1999，15，2： | 1 |
| 15 | Fourier transform mid-infrared (MIR) and near-infrared (NIR)spectroscopy for rapid quality assessment of chinese medicine preparation honghua oil显示文摘 | Wu Yanwen Sun Suqin Zhou Quu | 2008 | Journal of Pharmaceutical and Biomedical Analysis2008，46，3： | 1 |
| 16 | Liuwei Dihuang Lowers Body Weight and Improves Insulin and Leptin Sensitivity in Obese Rats显示文摘 | Benjamin Perry Junzeng Zhang Changhao Sun Tarek Saleh Yanwen Wang Vitaly Napadow | 2011 | Evidence-Based Complementary and Alternative Medicine2011，，： | 1 |
| 17 | Fourier transform midinfrared (MIR) and near-infrared (NIR) spectroscopy for rapid quality assessment of Chinese medicine preparation Honghua oil显示文摘 | WU Yanwen SUN Suqin ZHOU Qun | | 0，，03： | 1 |
| 18 | Direct Electrochemistry of Horseradish Peroxidase Immobilized in a Low Molecular Weight Gel显示文摘The ternary system of dodecylpyridinium bromide(DDPB)/acetone/H2O with appropriate composition can form a gel spontaneously and the gel is stable in hydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophos-phate([Bmim]PF_(6)).Based on the gelation phenomenon we observed,the low molecular weight gelator(LMWG)was first tried to immobilize horseradish peroxidase(HRP)on glassy carbon electrode(GCE).The scanning elec-tron microscope(SEM)images,the UV-Vis spectra and the bioactivity measurement indicate that the gel is suitable for the immobilization of HRP.The direct electrochemistry of the HRP-gel modified GCE(HRP-gel/GCE)in[Bmim]PF_(6)shows a pair of well-defined and quasi-reversible redox peaks with the heterogeneous electron transfer rate constant(ks)being 14.4 s^(−1),indicating that the direct electron transfer between HRP and GCE is fast.The HRP-gel/GCE is stable and reproducible.Also the electrode exhibits good electrocatalytic effect on the reduction of trichloroacetic acid(TCA),showing good promise in bioelectrocatalysis. | Yan Hu Yanwen Sun Lu Lu Xirong Huang | 2014 | Chinese Journal of Chemistry2014，32，3： | 0 |
| 19 | Enhancement of SARS-CoV-2 vaccine-induced immunity by a Toll-like receptor 7 agonist adjuvant显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is an enveloped positive-sense single-stranded RNA(ssRNA)virus responsible for the global COVID-19 pandemic.Although vaccines for SARS-CoV-2 have been developed and applied worldwide,research on vaccine adjuvant is lacking.Viral infections normally elicit a range of innate immune responses through the activation of pattern recognition receptors(PRRs). | Gen Li Meixing Yu Qiong Ke Jing Sun Yanwen Peng Chuanfeng Xiong Olivia Monteiro COVID-Infection and Immunity Investigation Group Jincun Zhao Andy P.Xiang | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 0 |
| 20 | Establishment, characterization, and application of pAcr-SP-NTP-EGFP transgenic mice in visualizing the oviduct-migrating ability of sperm from Prss37-null mice显示文摘 | Chunling Shen Jinjin Wang Hua Zhuang Jianbing Liu Xiyi Wang Xuejiao Chen Zhuanbin Wu Wenting Wu Youbing Wu Yanwen Sun Huimin Yan Jian Fei Ying Kuang Zhugang Wang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，6： | 0 |
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