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| 1 | The Arabidopsis P450 protein CYP82C2 modulates jasmonateinduced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis显示文摘Jasmonic 酸(JA ) 是对草食动物和一些微生物引起的病原体调整大量植物防卫回答的一个丰满的导出酸的发信号分子。分子的基因研究证实了 JA 也在植物开发的几个方面执行一个关键角色。这里,我们描述 Arabidopsis 变异的 jasmonic acid-hypersensitive1-1 (jah1-1 ) 的描述,它在 JA 回答的几个方面是有缺点的。尽管异种在根生长抑制展出增加的敏感到 JA,它显示出 JA 可诱导的防卫基因和减少的抵抗的减少的表示到 necrotrophic 真菌 Botrytis cinerea。基因克隆研究显示这些缺点被一个变化在细胞色素 P450 蛋白质 CYP82C2 引起。我们提供证明的证据到 B 的 jah1-1 异种的损害抵抗。cinerea 被导致 JA 的防卫基因的减少的表示伴随并且减少了导致 JA 的吲哚 glucosinolates (IG ) 的累积。相反地,提高的抵抗到 B。在 CYP82C2-overexpressing 植物的 cinerea 被导致 JA 的防卫基因的增加的表示伴随并且提高了导致 JA 的 IG 的层次。我们证明 CYP82C2 影响 IG biosynthetic 先锋的导致 JA 的累积色氨酸(Trp ) ,然而并非导致 JA 的 IAA 或导致病原体的 camalexin。一起,我们 CYP82C2 可以在导出 Trp 的第二等的代谢物的新陈代谢扮演在下面的一个假设在 JA 在哪个铺平调节的结果支持被提高。jah1-1 异种应该因此在向理解位于调停 JA 的微分回答的复杂性下面的机制的未来研究是重要的,它是重要的让植物使他们的生长适应千变万化环境。 | Fang Liu Hongling Jiang Songqing Ye Wen-Ping Chen Wenxing Liang Yingxiu Xu Bo Sun Jiaqiang Sun Qiaomei Wang Jerry D Cohen Chuanyou Li | 2010 | Cell Research2010，20，5： | 10 |
| 2 | Influence of reflow processing conditions on the uniformity of the chromium passivation film on tinplate显示文摘This study aims to systematically analyze the key parameters of the reflow process that influence the uniformity of the chromium passivation film coated on tinplate. The distribution characteristics of the chromium passivation film coated on the tinplate surface under different treatment conditions w ere systematically characterized using the scanning Kelvin probe technique,X-ray photoelectron spectroscopy,and X-ray diffraction. Results indicate that the use of flux reduces the porosity of tin coating,thereby favoring the uniform grow th of the passivation film. Furthermore,an increase in the reflow pow er and quenching temperature facilitates the homogeneous distribution of the passivation film on the tinplate surface,particularly w hen treated w ith electrolytic cathodic sodium dichromate. | XIE Long CHEN Hongxing XIE Yingxiu | 2014 | Baosteel Technical Research2014，8，2： | 5 |
| 3 | Genome-wide association mapping of vitamins B1 and B2 in common wheat显示文摘Vitamin B is essential for maintaining normal life activities in humans and animals who have to intake the microelement from the outside, especially from cereal products. In the present study 166 Chinese and foreign wheat cultivars planted in two environments were characterized for variation in vitamin B1 and B2 contents. A genome-wide association study(GWAS) using the wheat 90 K SNP assay identified 17 loci for vitamin B1 and 7 for vitamin B2 contents. Linear regression analysis showed a significantly positive correlation of the number of favorable alleles with vitamin B1 and B2 contents. Marker-trait associations(MTAs) at IWB43809(6AS, 0cM) and IWB69903(6AS, 13cM) were new and stable, and significantly associated with vitamin B1 content across two environments. The loci identified in this study and associated SNP markers could be used for improvement of vitamin B1 and B2 contents to obtain superior quality along with grain yield in wheat. | Jieyun Li Jindong Liu Wei'e Wen Pingzhi Zhang Yingxiu Wan Xianchun Xia Yan Zhang Zhonghu He | 2018 | The Crop Journal2018，6，3： | 4 |
| 4 | Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica显示文摘Genomic variants libraries are conducive to obtain dominant strains with desirable phenotypic traits.The non-homologous end joining(NHEJ),which enables foreign DNA fragments to be randomly integrated into different chromosomal sites,shows prominent capability in genomic libraries construction.In this study,we established an efficient NHEJ-mediated genomic library technology in Yarrowia lipolytica through regulation of NHEJ repair process,employment of defective Ura marker and optimization of iterative transformations,which enhanced genes integration efficiency by 4.67,22.74 and 1.87 times,respectively.We further applied this technology to create high lycopene producing strains by multi-integration of heterologous genes of CrtE,CrtB and CrtI,with 23.8 times higher production than rDNA integration through homologous recombination(HR).The NHEJ-mediated genomic library technology also achieved random and scattered integration of loxP and vox sites,with the copy number up to 65 and 53,respectively,creating potential for further application of recombinase mediated genome rearrangement in Y.lipolytica.This work provides a high-efficient NHEJ-mediated genomic library technology,which enables random and scattered genomic integration of multiple heterologous fragments and rapid generation of diverse strains with superior phenotypes within 96 h.This novel technology also lays an excellent foundation for the development of other genetic technologies in Y.lipolytica. | Qiuyan Bai Shuai Cheng Jinlai Zhang Mengxu Li Yingxiu Cao Yingjin Yuan | 2021 | Science China(Life Sciences)2021，64，12： | 4 |
| 5 | Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice显示文摘Drought is a major factor restricting the production of rice(Oryza sativa L.).The identification of natural variants for drought stress-related genes is an important step toward developing genetically improved rice varieties.Here,we characterized a member of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)family,OsSPL10,as a transcription factor involved in the regulation of drought tolerance in rice.OsSPL10 appears to play a vital role in drought tolerance by controlling reactive oxygen species(ROS)production and stomatal movements.Haplotype and allele frequency analyses of OsSPL10 indicated that most upland rice and improved lowland rice varieties harbor the OsSPL10Hap1allele,whereas the OsSPL10Hap2allele was mainly present in lowland and landrace rice varieties.Importantly,we demonstrated that the varieties with the OsSPL10Hap1allele showed low expression levels of OsSPL10 and its downstream gene,OsNAC2,which decreases the expression of OsAP37 and increases the expression of OsCOX11,thus preventing ROS accumulation and programmed cell death(PCD).Furthermore,the knockdown or knockout of OsSPL10 induced fast stomatal closure and prevented water loss,thereby improving drought tolerance in rice.Based on these observations,we propose that OsSPL10 confers drought tolerance by regulating OsNAC2 expression and that OsSPL10Hap1could be a valuable haplotype for the genetic improvement of drought tolerance in rice. | Yingxiu Li Shichen Han Xingming Sun Najeeb Ullah Khan Qun Zhong Zhanying Zhang Hongliang Zhang Feng Ming Zichao Li Jinjie Li | 2023 | Journal of Integrative Plant Biology2023，65，4： | 2 |
| 6 | Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes显示文摘Trichomes function in plant defenses against biotic and abiotic stresses;examination of glabrous lines,which lack trichomes,has revealed key aspects of trichome development and function.Tests of allelism in 51 glabrous rice(Oryza sativa)accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice.Here,we report that OsSPL10 acts as a transcriptional regulator controlling trichome development.Haplotype and transient expression analyses revealed that variation in the approximately 700-bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD-17993.Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL-HL6 background.Plants with genotype OsSPL10^(WD-17993)/HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the glumes.HAIRY LEAF6(HL6)encodes another transcription factor that regulates trichome initiation and elongation,and OsSPL10 directly binds to the HL6 promoter to regulate its expression.Moreover,the transcript levels of auxin-related genes,such as OsYUCCA5 and OsPIN-FORMED1b,were altered in OsSPL10 overexpression and RNAi transgenic lines.Feeding tests using locusts(Locusta migratoria)demonstrated that non-glandular trichomes affect feeding by this herbivore.Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions. | Jinjie Li Bo Tang Yingxiu Li Chenguang Li Minjie Guo Haiyang Chen Shichen Han Jin Li Qijin Lou Wenqiang Sun Peng Wang Haifeng Guo Wei Ye Zhanying Zhang Hongliang Zhang Sibin Yu Long Zhang Zichao Li | 2021 | Journal of Integrative Plant Biology2021，63，8： | 2 |
| 7 | Analysis and Evaluation of Grain Quality in Main Wheat Production Areas of Anhui Province显示文摘In order to clarify the status of grain quality in the main wheat production areas of Anhui Province,wheat quality sampling and testing were carried out in the main wheat production areas of Anhui Province in 2015,26 cultivars and 62 samples were collected,and grain traits,quality indexes and quality traits were analyzed and evaluated.The average bulk density of the sample was 796 g/L; the average hardness index was 49.2; the average 1 000-grain weight was 41.6 g; the average grain moisture content was 11.8%; the average crude protein content was 11.7%; the average wet gluten content was 31.1%; the average Zeleny sedimentation value was 24 m L; the average water absorption was 54.9%; the average stability time was 5.6 min.The crude protein content of 35.5% samples reached the standard of high quality weak gluten; the wet gluten content of 40.3% samples was above the second grade of high quality strong gluten; the falling number of 83.9% of the samples reached the national standard of high quality wheat; the stability time of 27.4% of the samples reached higher than the second grade of high quality gluten,and the stability time of 17.7% samples reached the standard of high quality weak gluten wheat. | Qiqi ZHANG Yingxiu WAN Wenxin CAO Yan LI Yao LI Pingzhi ZHANG | 2018 | Asian Agricultural Research2018，10，7： | 1 |
| 8 | The bHLH-type transcription factor AtAIB positively regulates ABA response in Arabidopsis显示文摘 | Hongmei Li Jiaqiang Sun Yingxiu Xu Hongling Jiang Xiaoyan Wu Chuanyou Li | 2007 | Plant Molecular Biology2007，，5： | 1 |
| 9 | Flow injection determination of dopamine based on inhibited electrochemiluminescence of luminol显示文摘 | ZHU Liande LI Yingxiu ZHU Guoyi | 2002 | Analytical Letters2002，35，15： | 1 |
| 10 | Young but not relatively old retrotransposons are preferentially located in gene‐rich euchromatic regions in tomato ( Solanum lycopersicum ) plants显示文摘 | Yingxiu Xu Jianchang Du | 2014 | Plant J2014，，4： | 1 |
| 11 | Highly efficient multiplex base editing:One-shot deactivation of eight genes in Shewanella oneidensis MR-1显示文摘Obtaining electroactive microbes capable of efficient extracellular electron transfer is a large undertaking for the scalability of bio-electrochemical systems.Inevitably,researchers need to pursue the co-modification of multiple genes rather than expecting that modification of a single gene would make a significant contribution to improving extracellular electron transfer rates.Base editing has enabled highly-efficient gene deactivation in model electroactive microbe Shewanella oneidensis MR-1.Since multiplexed application of base editing is still limited by its low throughput procedure,we thus here develop a rapid and efficient multiplex base editing system in S.oneidensis.Four approaches to express multiple gRNAs were assessed firstly,and transcription of each gRNA cassette into a monocistronic unit was validated as a more favorable option than transcription of multiple gRNAs into a polycistronic cluster.Then,a smart scheme was designed to deliver one-pot assembly of multiple gRNAs.3,5,and 8 genes were deactivated using this system with editing efficiency of 83.3%,100%and 12.5%,respectively.To offer some nonrepetitive components as alternatives genetic parts of sgRNA cassette,different promoters,handles,and terminators were screened.This multiplex base editing tool was finally adopted to simultaneously deactivate eight genes that were identified as significantly downregulated targets in transcriptome analysis of riboflavin-overproducing strain and control strain.The maximum power density of the multiplex engineered strain HRF(8BE)in microbial fuel cells was 1108.1 mW/m2,which was 21.67 times higher than that of the wild-type strain.This highly efficient multiplexed base editing tool elevates our ability of genome manipulation and combinatorial engineering in Shewanella,and may provide valuable insights in fundamental and applied research of extracellular electron transfer. | Yaru Chen Meijie Cheng Yan Li Lin Wang Lixia Fang Yingxiu Cao Hao Song | 2023 | Synthetic and Systems Biotechnology2023，8，1： | 1 |
| 12 | Electrochemiluminescent Determination of L - Cysteine with a Flow - Injection Analysis System 显示文摘 | Liande Zhu Yingxiu Li Guoyi Zhu | 2003 | Analytical Sciences2003，19，4： | 1 |
| 13 | The bHLH-type transcription factor AtAIB positively regulates ABA response in Arabidopsis显示文摘 | Hongmei Li Jiaqiang Sun Yingxiu Xu Hongling Jiang Xiaoyan Wu Chuanyou Li | 2007 | Plant Molecular Biology2007，，5： | 1 |
| 14 | A novel renewable plant tissue-based electrochemiluminescent biosensor for glycolic acid显示文摘 | Zhu Liande Li Yingxiu Zhu Guoyi | 2004 | Sensors and Actuators B2004，98，： | 1 |
| 15 | The bHLH-type transcription factor AtAIB positively regulates ABA response in Arabidopsis显示文摘 | Hongmei Li Jiaqiang Sun Yingxiu Xu Hongling Jiang Xiaoyan Wu Chuanyou Li | 2007 | Plant Molecular Biology2007，，5： | 1 |
| 16 | Conductive proteins-based extracellular electron transfer of electroactive microorganisms显示文摘Electroactive microorganisms(EAMs)could utilize extracellular electron transfer(EET)pathways to exchange electrons and energy with their external surroundings.Conductive cytochrome proteins and nanowires play crucial roles in controlling electron transfer rate from cytosol to extracellular electrode.Many previous studies elucidated how the c-type cytochrome proteins and conductive nanowires are synthesized,assembled,and engineered to manipulate the EET rate,and quantified the kinetic processes of electron generation and EET.Here,we firstly overview the electron transfer pathways of EAMs and quantify the kinetic parameters that dictating intracellular electron production and EET.Secondly,we systematically review the structure,conductivity mechanisms,and engineering strategies to manipulate conductive cytochromes and nanowire in EAMs.Lastly,we outlook potential directions for future research in cytochromes and conductive nanowires for enhanced electron transfer.This article reviews the quantitative kinetics of intracellular electron production and EET,and the contribution of engineered c-type cytochromes and conductive nanowire in enhancing the EET rate,which lay the foundation for enhancing electron transfer capacity of EAMs. | Junqi Zhang Zixuan You Dingyuan Liu Rui Tang Chao Zhao Yingxiu Cao Feng Li Hao Song | 2023 | Quantitative Biology2023，11，4： | 0 |
| 17 | Major Chemical Synthesis Methods and Application Progress of Sex Pheromone of Asian Corn Borer显示文摘This article reviews the main chemical synthesis methods of sex pheromone of Asian corn borer, common strategies for constructing dou-ble bond, molecular composition and field application progress of sex pheromone of Asian corn borer, which will provide reference for biological control of Asian corn borer. | Jiang Zhongping Li Jintao Wang Yingxiu Zhang Zuoshan Liu Qinsheng Wang Xia | 2020 | Plant Diseases and Pests2020，11，1： | 0 |
| 18 | Research and Application of Synchronization Update of Distributed Heterogeneous Database显示文摘 | Xin Yingxiu | 2014 | International Journal of Technology Management2014，，5： | 0 |
| 19 | A novel MYCN-YTHDF1 cascade contributes to retinoblastoma tumor growth by eliciting m^(6)A-dependent activation of multiple oncogenes显示文摘Retinoblastoma, the most prevalent primary intraocular tumor in children, leads to vision impairment, disability and even death.In addition to RB1 inactivation, MYCN activation has been documented as another common oncogenic alteration in retinoblastoma and represents one of the high-risk molecular subtypes of retinoblastoma. However, how MYCN contributes to the progression of retinoblastoma is still incompletely understood. Here, we report that MYCN upregulates YTHDF1, which encodes one of the reader proteins for N6-methyladenosine(m^(6)A) RNA modification, in retinoblastoma. We further found that this MYCN-upregulated m^(6)A reader functions to promote retinoblastoma cell proliferation and tumor growth in an m^(6)A bindingdependent manner. Mechanistically, YTHDF1 promotes the expression of multiple oncogenes by binding to their m RNAs and enhancing m RNA stability and translation in retinoblastoma cells. Taken together, our findings reveal a novel MYCN-YTHDF1regulatory cascade in controlling retinoblastoma cell proliferation and tumor growth, pinpointing an unprecedented mechanism for MYCN amplification and/or activation to promote retinoblastoma progression. | Yingxiu Luo Mengjia He Jie Yang Feifei Zhang Jie Chen Xuyang Wen Jiayan Fan Xianqun Fan Peiwei Chai Renbing Jia | 2023 | Science China(Life Sciences)2023，66，9： | 0 |
| 20 | An inspection on the Gini coefficient of the budget educational public expenditure per student for China's basic education显示文摘Using statistical data on the implementing conditions of China's educational expenditure published by the state,this paper studies the Gini coefficient of the budget educational public expenditure per student in order to examine the concentration degree of the educational expenditure for China's basic education and analyze its balanced development condition.As the research shows,China's basic education is undergoing an unbalanced development due to diversified factors,which is mainly reflected as follows:firstly,the budget educational public expenditure presents a four-tiered appearance of the strong,the less strong,the less weak and the weak,which lead to a great discrepancy between the two opposing extremes;secondly,the compulsory education in rural areas is still confronted with great difficulties;thirdly,the general senior secondary education is loaded with the crisis of unbalance.Therefore,it is necessary to construct a balanced development policy framework of the basic education and pay close attention to the benefit and effectiveness of the educational input.In addition,it is also important to clearly stipulate the criterion of the government's educational allocation and to support the disadvantaged areas in order to promote the balanced development of the basic education. | Yang Yingxiu | 2006 | Frontiers of Education in China2006，1，2： | 0 |
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