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| 1 | Mechanical property of artificial methane hydrate under triaxial compression显示文摘Methane production from hydrate reservoir may induce seabed slide and deformation of the hydrate-bearing strata.The research on mechanical properties of methane hydrate is considered to be important for developing an efficient methane exploitation technology.In this paper,a triaxial test system containing a pressure crystal device was developed with the conditions to stabilize the hydrate.A series of triaxial shear tests were carried out on artificial methane hydrate specimen.In addition,mechanical characteristics of methane hydrate were studied with the strain rates of 0.1 and 1.0 mm/min,respectively,under the conditions of different temperatures(T =-5,-10,and-20 ℃) and confining pressures(P = 0,5,10,15,and 20 MPa).The preliminary results show that when the confining pressure was less than 10 MPa,the increase of confining pressure leaded to the enhancement of shear strength.Furthermore,the decreasing temperature and the increasing strain rate both caused the increase in shear strength. | Yongchen Song Feng Yu Yanghui Li Weiguo Liu Jiafei Zhao | 2010 | Journal of Natural Gas Chemistry2010,19,3: | 28 |
| 2 | Difference of gene expression profiles between esophageal carcinoma and its pericancerous epithelium by gene chip显示文摘AIM: To study the difference of gene expression between esophageal carcinoma and its pericancerous epithelium and to screen novel associated genes in the early stage of esophageal carcinogenesis by cDNA microarray.METHODS: Total RNA was extracted with the original single step way from esophageal carcinoma, its pericancerous epithelial tissue and normal esophageal epithelium far from the tumor. The cDNA retro-transcribed from equal quantity of mRNA was labeled with Cy5 and Cy3 fluorescence functioning as probes. The mixed probes were hybridized with two pieces of BioDoor 4 096 double dot human whole gene chip. Fluorescence signals were scanned by ScanArray 3 000 laser scanner and farther analyzed by ImaGene 3.0software with the digital computer.RESULTS: (1) A total of 135 genes were screened out, in which 85 and 50 genes whose the gene expression levels (fluorescence intensity) in esophageal carcinoma were more than 2 times and less than 0.5 times respectively compared with the normal esophageal epithelium. (2) There were also total 31 genes, among then 27 and 4 whose expressions in pericancerous tissue were 2-fold up-regulated and 0.5-fold down-regulated respectively compared with normal esophageal epithelium. (3) There were 13 genes appeared simultaneously in both pericancerous epithelium and esophageal carcinoma, while another 18 genes existed in pericancerous epithelium only.CONCLUSION: With the parallel comparison among these three gene profiles, it was shown that (1). A total of 135genes, Whose expression difference manifested as fluorescence intensity were more than 2 times between esophageal carcinoma and normal esophageal epithelium,were probably related to the occurrence and development of the esophageal carcinoma. (2). The 31 genes showing expression difference more than 2 times between pericancerous and normal esophageal epithelium might be relate to the promotion of esophageal pericancerosis and its progress. The present study illustrated that by using the gene chip to detect the difference of gene expression profiles might be of benefit to the gene diagnosis, treatment and prevention of esophageal carcinoma. | Shen-HuaXu Li-JuanQian Han-ZhouMou Chi-HongZhu Xing-MingZhou Xiang-LinLiu YongChen Wen-YuBao | 2003 | World Journal of Gastroenterology2003,9,3: | 23 |
| 3 | Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction显示文摘 | PengXia JinhuaZhou Xiaoyu Song BingWu XingLiu Di Li Shuyuan Zhang Zhikai Wang H uijuan Yu Tarsha Ward Jiancun Zhang Yinmei Li Xiaoning Wang YongChen Zhen Guo Xuebiao Yao | 2014 | Journal of Molecular Cell Biology2014,8,3: | 9 |
| 4 | Study of the permeability characteristics of porous media with methane hydrate by pore network model显示文摘The permeability in the methane hydrate reservoir is one of the key parameters in estimating the gas production performance and the flow behavior of gas and water during dissociation.In this paper,a three-dimensional cubic pore-network model based on invasion percolation is developed to study the effect of hydrate particle formation and growth habit on the permeability.The variation of permeability in porous media with different hydrate saturation is studied by solving the network problem.The simulation results are well consistent with the experimental data.The proposed model predicts that the permeability will reduce exponentially with the increase of hydrate saturation,which is crucial in developing a deeper understanding of the mechanism of hydrate formation and dissociation in porous media. | Haifeng Liang Yongchen Song Yu Liu Mingjun Yang Xing Huang | 2010 | Journal of Natural Gas Chemistry2010,19,3: | 7 |
| 5 | Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites显示文摘MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions. | WANG Ketao SU Xiaomei CUI Xia DU Yongchen ZHANG Shuaibin GAO Jianchang | 2018 | Horticultural Plant Journal2018,4,2: | 6 |
| 6 | Identification of Regulatory DNA Elements Using Genome-wide Mapping of DNase I Hypersensitive Sites during Tomato Fruit Development显示文摘开发并且西红柿水果成熟被 transcriptional 规定精确控制,它取决于规章的蛋白质的安排可接近性到倡导者和另外的 cis 规章的 DNA 元素。基因表示上的这可接近性和它的效果在定义发展进程起一个主要作用。为了理解规章的机制和词法、解剖的功能的元素 modulating,在水果开发期间变化,我们产生了 DNase 的染色体宽的高分辨率的地图我从西红柿栽培变种 “Moneymaker” 的水果纸巾的过分敏感的地点(DHS )在 20 点,天张贴开花期以及裂缝舞台。由越过水果发展阶段探索 DHS 的变化,我们指出了与发展特定的基因有关的最可能的过分敏感的地点。由在维生素酸 biosynthetic 小径在这些发展特定的基因或基因的 DHS 上检测有约束力的主题,我们揭示了贡献协调植物成熟的基因抄写的普通规章的元素并且专业化新陈代谢的小径。我们的结果贡献一更好涉及西红柿水果开发的基因的规章的动力学理解并且成熟。 | Zhengkun Qiu Ren Li Shuaibin Zhang Ketao Wang Meng Xu Jiayang Li Yongchen Du Hong YU Xia Cui | 2016 | Molecular Plant2016,9,8: | 6 |
| 7 | In-situ observation for formation and dissociation of carbon dioxide hydrate in porous media by magnetic resonance imaging显示文摘The study of formation and dissociation of CO 2 hydrate in porous media was characterized by magnetic resonance imaging (MRI) system in in situ conditions. This work simulated porous media by using glass beads of uniform size. The growth and dissociation habit of CO2 hydrate was observed under different temperature and pressure conditions. The induction time and the hydrate saturation during the growth and dissociation process in different sizes of porous media were obtained by using the MRI signal intensity. The results indicate that hydrate growth rate and the induction time are affected by the size of porous media, pressure, and degree of supercooling. There are three hydrate growth stages, i.e., initial growth stage, rapid growth stage and steady stage. In this study,the CO2 hydrate forms preferentially at the surface of vessel and then gradually grows inward. The hydrate tends to cement the glass beads together and occupies the pore gradually. As the hydrate decomposes gradually, the dissociation rate increases to the maximum and then decreases to zero. | CHENG ChuanXiao ZHAO JiaFei SONG YongChen ZHU ZiHao LIU WeiGuo ZHANG Yi YANG MingJun YU XiChong | 2013 | Science China Earth Sciences2013,56,4: | 5 |
| 8 | Sediment Transport in the Nearshore Area of Phoenix Island显示文摘Based on the measured data, suspended sediment concentration, surface sediment grain size, current and waves, the sediment transport mechanisms and pathways in the Phoenix Island area were analyzed using methods of flux decomposition and Grain Size Trend Analysis(GSTA). The results show that net suspended sediment is mainly transported by average current, Stokes drift, and gravitational circulation. The transport direction of suspended sediment is varying and basically following the direction of residual tidal currents. Surface sediment transport pathways are primarily parallel to the coastline along with two convergent centers. Waves and longshore currents have a significant influence on sediment transport, but the influence is limited due to a steep and deep underwater bank. Tidal current is the main controlling factor for sediment transport, especially in the deep water area. Neither suspended nor surface sediment is transported towards the southwest. The South Shandong Coastal Current(SSCC) has little effect on sediment transport processes in the nearshore area of Phoenix Island. | HU Rijun MA Fang WU Jianzheng ZHANG Wei JIANG Shenghui XU Yongchen ZHU Longhai WANG Nan LIU Aijiang | 2016 | Journal of Ocean University of China2016,15,5: | 4 |
| 9 | Forced Convection Heat Transfer in Porous Structure: Effect of Morphology on Pressure Drop and Heat Transfer Coefficient显示文摘A light-weight structure with sufficient mechanical strength and heat transfer performance is increasingly required for some thermal management issues.The porous structure with the skeleton supporting the ambient stress and the pores holding the flowing fluid is considered very promising,attracting significant scientific and industrial interest over the past few decades.However,due to complicated morphology of the porous matrices and thereby various performance of the pressure drop and heat transfer coefficients (HTC),the comprehensive comparison and evaluation between different structures are largely unclear.In this work,recent researches on the efforts of forced convection heat transfer in light-weight porous structure are reviewed;special interest is placed in the open-cell foam,lattice-frame,structured packed bed,and wire-woven structures.Their experimental apparatus,morphological of the porous structures,effect of morphology on pressure drop and HTC,and further applications are discussed.The new method which measure morphology accurately should be paid more attention to develop more accuracy correlation.Also,the most research focused on low Reynolds number and existing structure,while very few researchers investigated the property of forced convection heat transfer in high velocity region and developed new porous structure. | ZHAO Jiafei SUN Mingrui ZHANG Lunxiang HU Chengzhi TANG Dawei YANG Lei SONG Yongchen | 2021 | Journal of Thermal Science2021,30,2: | 4 |
| 10 | Numerical studies of hydrate dissociation and gas production behavior in porous media during depressurization process显示文摘In this study,a numerical model is developed to investigate the hydrate dissociation and gas production in porous media by depressurization.A series of simulation runs are conducted to study the impacts of permeability characteristics,including permeability reduction exponent,absolute permeability,hydrate accumulation habits and hydrate saturation,sand average grain size and irreducible water saturation.The effects of the distribution of hydrate in porous media are examined by adapting conceptual models of hydrate accumulation habits into simulations to govern the evolution of permeability with hydrate decomposition,which is also compared with the conventional reservoir permeability model,i.e.Corey model.The simulations show that the hydrate dissociation rate increases with the decrease of permeability reduction exponent,hydrate saturation and the sand average grain size.Compared with the conceptual models of hydrate accumulation habits,our simulations indicate that Corey model overpredicts the gas production and the performance of hydrate coating models is superior to that of hydrate filling models in gas production,which behavior does follow by the order of capillary coating>pore coating>pore filling>capillary filling.From the analysis of t1/2,some interesting results are suggested as follows:(1) there is a 'switch' value(the'switch'absolute permeability) for laboratory-scale hydrate dissociation in porous media,the absolute permeability has almost no influence on the gas production behavior when the permeability exceeds the 'switch' value.In this study,the 'switch' value of absolute permeability can be estimated to be between 10 and 50 md.(2) An optimum value of initial effective water saturation Sw,e exists where hydrate dissociation rate reaches the maximum and the optimum value largely coincides with the value of irreducible water saturation S wr,e.For the case of Sw,Swr,e,there are different control mechanisms dominating the process of hydrate dissociation and gas production. | Xuke Ruan Mingjun Yang Yongchen Song Haifeng Liang Yanghui Li | 2012 | Journal of Natural Gas Chemistry2012,21,4: | 3 |
| 11 | Shaped binderless ZSM-11 zeolite catalyst prepared via a dry-gel conversion method:Characterization and application for alkylation of benzene with dimethyl ether显示文摘Shaped binderless ZSM-11 zeolite catalysts were synthesized via a dry-gel conversion technique from 70ZSM-11/30 SiO_2mix extrudates. 1,6-hexanediamine combined with tetrabutylammonium bromide was proved to be the best structure directing agent for the synthesis of the binderless ZSM-11 catalyst, without adding other alkaline materials. The 70HZSM-11/30 SiO_2mix serials materials crystallized for different times were detected by X-ray diffraction(XRD), nuclear magnetic resonance(NMR), scanning electron microscopy(SEM), transmission electron microscopy(TEM), scanning transmission electron microscopy–energy dispersive spectroscopy(STEM–EDS) techniques, and so on. In order to investigate the possible crystallization mechanism, the textural and structural properties of 70HZSM-11/30 SiO_2mix serials samples were further characterized by N_2 adsorption–desorption. Acid properties were determined by temperature-programed desorption of NH_3(NH_3-TPD) and pyridine adsorption-infrared(Py-IR) measurements. In the alkylation of benzene with dimethyl ether, the serials catalysts exhibited different benzene conversions. 70HZSM-11/30 SiO_2mix showed the lowest benzene conversion while sample 70HZSM-11/30 SiO_2mix-6.5h synthesized only for 6.5h displayed a higher benzene conversion, even higher than the value over 70HZSM-11/30Al_2O_3mix. Extending the crystallization time, the obtained samples displayed the increased benzene conversion in general under the same reaction conditions. In the end, the relation of physicochemical properties with the reaction performance was investigated. | Wanshuo Zhang Shuang Zhang Wenjie Xin Hui Liu Yongchen Shang Xiangxue Zhu Shenglin Liu Longya Xu | 2017 | Journal of Energy Chemistry2017,26,3: | 3 |
| 12 | Water permeability in MXene membranes: Process matters显示文摘Recent studies have shown impressive transport behaviors of water and ions within lamellar MXene membranes,which endows great promise in developing advanced separation application based high performance MXene membranes.However,most of the researches focused on modification of MXene nanoflakes and optimizing interlayer distance,leaving the impact of membrane fabrication process marginal.In this work,we studied the water flux of membranes made by vacuum filtration using delaminated MXene nanoflakes as the building-blocks.Our results show that the water permeability is extremely sensitive to the process,especially at the drying process,loading and deposit rate of nanoflakes(the feeding concentration).We find that the voids from less ordered stack rather than in-plane defects and interlayer galleries contribute to the large water permeability.The voids can be effectively avoided via deposition of MXene nanoflakes at a slow rate.Manipulating the stack of MXene nanoflakes during vacuum filtration and drying are critical for development of MXene membranes with desired performance for water permeation. | Hang Zhou Yuwei Wang Fuqiang Wang Hao Deng Yongchen Song Changping Li Zheng Ling | 2020 | Chinese Chemical Letters2020,31,6: | 3 |
| 13 | A putative bHLH transcription factor is a candidate gene for male sterile 32,a locus affecting pollen and tapetum development in tomato显示文摘The tomato(Solanum lycopersicum)male sterile 32(ms32)mutant has been used in hybrid seed breeding programs largely because it produces no pollen and has exserted stigmas.In this study,histological examination of anthers revealed dysfunctional pollen and tapetum development in the ms32 mutant.The ms32 locus was fine mapped to a 28.5 kb interval that encoded four putative genes.Solyc01g081100,a homolog of Arabidopsis bHLH10/89/90 and rice EAT1,was proposed to be the candidate gene of MS32 because it contained a single nucleotide polymorphism(SNP)that led to the formation of a premature stop codon.A codominant derived cleaved amplified polymorphic sequence(dCAPS)marker,MS32D,was developed based on the SNP.Real-time quantitative reverse-transcription PCR showed that most of the genes,which were proposed to be involved in pollen and tapetum development in tomato,were downregulated in the ms32 mutant.These findings may aid in marker-assisted selection of ms32 in hybrid breeding programs and facilitate studies on the regulatory mechanisms of pollen and tapetum development in tomato. | Xiaoyan Liu Mengxia Yang Xiaolin Liu Kai Wei Xue Cao Xiaotian Wang Xiaoxuan Wang Yanmei Guo Yongchen Du Junming Li Lei Liu Jinshuai Shu Yong Qin Zejun Huang | 2019 | Horticulture Research2019,6,1: | 3 |
| 14 | Nano functional neural interfaces显示文摘 | Yongchen Wang Hanlin Zhu Huiran Yang Aaron D. Argall Lan Luan Chong Xie Liang Guo | 2018 | Nano Research2018,11,10: | 2 |
| 15 | A method to refine the start-up scheme of compression and refrigeration systems in ethylene plants via dynamic simulation显示文摘Starting the cracking gas compressor and precooling the refrigeration system are keys to start-up of an ethylene plant and accounts for up to 50% of the total start-up time and plant flare emissions. Premature feeding of cracking furnaces can be avoided if the cracking gas compressor is started and the refrigeration system is precooled in advance using mixed gas as the start-up working medium(SWM). Start-up scenario with mixed gas as SWM could significantly reduce the emission loss and shorten the precooling time. Research shows that making appropriate start-up scheme is important not only to ensure operational safety and feasibility, but also to reduce energy consumption. In this paper, a method is proposed to select suitable start-up operational parameters of compression and refrigeration system with sufficient safe operating ranges and short precooling time. The complex interrelation among key parameters of start-up is analyzed. It is found that higher energy consumption, especially for super high-pressure steam(SS), can promote operational safety and shorten the precooling time during start-up.Based on steady-state and dynamic simulation, appropriate operating parameter ranges are determined with reasonable SS consumption. A real case study demonstrates that an appropriate start-up scheme will optimize the operation. | Shengfu Zhang Yongchen Zhao Tong Qiu Guang Song Jinsong Zhao | 2016 | Chinese Journal of Chemical Engineering2016,24,8: | 2 |
| 16 | Adsorption isotherms and kinetic characteristics of methane on block anthracite over a wide pressure range显示文摘It is important to quantitatively understand the methane adsorption and transport mechanism in coal for an evaluation of the reserves and for its production forecast. In this work, a block coal sample was chosen to perform the CH4 adsorption experiments using the gravimetric method at temperatures of 293.60 K, 311.26 K, 332.98 K and 352.55 K and pressures up to 19 MPa. The excess adsorption capacity of CH4 in dry block anthracite increased, followed by a sequence decrease with the increasing pressure. High temperature restrained the growth of the excess adsorption due to that the adsorption is an intrinsically physical and exothermic process. The excess adsorption peak decreased slowly with the increase of temperature and intersected at a pressure of more than 18 MPa; meanwhile, the pressure at the excess adsorption peak increased.The existing correlations were examined in terms of density rather than pressure. The DR+k correlation, with an average relative deviation of ±0.51%, fitted our data better than the others, with an average relative deviation of up to 2.29%. The transportation characteristics of CH4 adsorption was also investigated in this study, including the adsorption rate and diffusion in block coal. The kinetic data could be described by a modified unipore model. The adsorption rates were found to exhibit dependence on pressure and temperature at low pressures, while the calculated diffusivities exhibited little temperature dependence. In addition, the kinetic characteristics were compared between CH4 and CO2 adsorption on the block coal. The excess adsorption ratios of CO2 to CH4obtained from the DR+k model decreased with the increasing pressure. | Wanli Xing Yongchen Song Yi Zhang Weiguo Liu Lanlan Jiang Yanghui Li Yuechao Zhao | 2015 | Journal of Energy Chemistry2015,24,2: | 2 |
| 17 | Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoning显示文摘AIM: To construct pEGFP-hepatocyte growth factor (HGF) expression vector, the to detect its expression in transfected human hepatocytes, and to investigate the influence of autocrine HGF expression on the proliferative potential and cytoprotective effects in human hepatocytes.METHODS: Human HGF cDNA was ligated to the pEGFP vector. Recombinant plasmid was transfected into human hepatocyte line QZG with liposome. Expression of HGF protein was observed by fluorescence microscopy and immunohistochemistry. Hepatic cells were collected 24, 48, and 72 h after transfection to detect the number of [^3H]-TdR uptake in DNA. DNA synthesis was observed by using PCNA stain immunohistochemistry.Acute liver cell damage was induced by carbon tetrachloride. Cytoprotective effect was observed by examining the survival rate of hepatocytes and leakage of intracellular alanine transaminase (ALT) and potassium ions.RESULTS: HGF identification of pEGFP-HGF by enzyme digestion showed that HGF fragment was cloned into BamH I and SalI sites of pEGFP-N3. Expression of GFP in transfected hepatocytes was observed with fluorescence microscopy. The [^3H]-TdR uptake became 7 times as many as in thecontrol group 96 h after transfection. After HGF transfection, the survival rate of hepatocytes poisoned by CCI4 significantly increased (83% vs 61%, P<0.05), and the leakage of intracellular alanine transaminase and potassium ions decreased (586 nkat/L vs 1089 nkat/L, P<0.01; and 5.59 mmol/L vs 6.02 mmol/L, P<0.01 respectively). Culture of transfected hepatic cells promoted the proliferation of other nontransfected cells.CONCLUSION: Transfected HGF is expressed in hepatic cells and has the activity of promoting cell division and protecting hepatic cells against poisoning. | YongHe JunZhou Ke-FengDou YongChen Qing-GuoYan Hai-lVlinLi | 2004 | World Journal of Gastroenterology2004,10,19: | 2 |
| 18 | The detection of tetrahydrofuran hydrate formation and saturation using magnetic resonance imaging technique显示文摘Tetrahydrofuran(THF) hydrate was formed in bulk as well as in glass beads pack with a mean diameter of 3.0 mm by controlling the temperature under ambient pressure.Images of THF hydrate formation procedure were obtained using the magnetic resonance imaging(MRI) technique.The experiment results showed that MRI is an effective method for the detection of hydrate formation.Saturation of hydrate formed both in bulk and glass beads can be confirmed by intensity integration of MRI images. | Yu Liu Yongchen Song Yongjun Chen Lei Yao Qingping Li | 2010 | Journal of Natural Gas Chemistry2010,19,3: | 2 |
| 19 | Acid properties and catalytic performance for phosphonic acid modified MCM-49 zeolites in skeletal isomerization of n-butene 显示文摘 | Jin Miao Shang Yongchen Yang Piaoping | 2008 | Catalysis Communications2008,10,2: | 1 |
| 20 | A long pathlength spectrophotometric pCO2 sensor using a gas-permeable liquid-core waveguide显示文摘 | Zhaohui (Aleck) Wang Yongchen Wang Wei-Jun Cai Su-Yi Liu | 2002 | Talanta2002,57,: | 1 |