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| 1 | Clinical application of a new device for minimally invasive circumcision显示文摘瞄准:与 phimosis 或过量包皮在不同年龄的男病人的治疗学习一台可处理的割礼设备的临床的效果。方法:在 5 和 95 年的年龄之间的 1200 个病人在在 2005 年 10 月和 2007 年 9 月之间的 2 年的时期用这个过程经历了割礼。这些盒子, 904 有过量包皮, 296 是 phimosis 的盒子。结果:在 96.33% 盒子中,切口愈合了,离开没有使好化妆结果留下疤痕并且生产的内部包皮的最小的数量。没有到系带的设备脱臼或损坏的事件。平均起作用的时间为 phimosis 是为过量包皮,和 3.5 min 的 2.5 min。在穿设备的 7 天期间,对中等浮肿温和与 phimosis 与过量包皮并且在那些的 2.58 % 发生在盒子的 10.08 % 。在系带的浮肿在 1.67% 病人被看见,并且仅仅, 0.67% 有切口的感染。86.25% 病人的一个总数由于阴茎勃起报导了疼痛。在设备的移动以后, 0.58% 案例有在切口附近的最小的流血,并且 2.42% 弯屈裂开。结论:新设备能与 phimosis 和过量包皮被用于病人的一个压到优势的多数。这种技术相对简单表现,并且经历了这个手术的病人有很少的复杂并发症。抗菌素没比用常规方法被割除的那些被要求,病人们报导了更少的疼痛。有这台设备的割礼要求最小的组织操作,并且比用常规技术的割礼更快、安全。 | Yi-Feng Peng Yue Cheng Guo-Yao Wang Suo-Qun Wang Chao Jia Ben-Hai Yang Ru Zhu Shu-Chuan Jian Qing-Wen Li Da-Wei Geng | 2008 | Asian Journal of Andrology2008，10，3： | 105 |
| 2 | Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase显示文摘包含 methyltransferase 建筑群的象 3 一样的 methyltransferase (METTL3 ) 催化 N6-methyladenosine (m6A ) 形成,一个新奇 epitranscriptomic 标记;然而,这建筑群的性质仍然保持大部分未知。这里,我们报导人的 m6A methyltransferase 建筑群, Wilm 的肿瘤 1 伙伴蛋白质(WTAP ) 和象 14 一样的 methyltransferase (METTL14 ) 的二个新部件。WTAP 与 METTL3 和 METTL14 交往,并且为他们的本地化被要求进在 vivo 与处理因素的 pre-mRNA 并且为 m6A methyltransferase 的催化活动充实的原子点缀。RNA 的多数在 vivo 由 WTAP 和 METTL3 跳了代表包含一致 m6A 主题的 mRNAs。当 WTAP 不在时, METTL3 的 RNA 有约束力的能力强烈被减少,建议 WTAP 可以工作调整到 mRNA 目标的 m6A methyltransferase 建筑群的招募。而且,在有 photoactivatable-ribonucleoside-enhanced crosslinking 和 immunoprecipitation (同等片断) 的联合的 transcriptomic 分析说明那 WTAP 和 METTL3 调整涉及抄写并且 RNA 处理的基因拼接的表示和选择。在 zebrafish 胚胎的调停 Morpholino 的击倒的指向 WTAP 或 METTL3 引起了织物区别缺点并且增加了 apoptosis。这些调查结果提供 WTAP 可以在 m6A methyltransferase 建筑群作为一个规章的子单元工作并且在 RNA 的 epitranscriptomic 规定起一个关键作用的充分证据新陈代谢。 | Xiao-Li Ping Bao-Fa Sun Lu Wang Wen Xiao Xin Yang Wen-Jia Wang Samir Adhikari Yue Shi Ying Lv Yu-Sheng Chen Xu Zhao Ang Li Ying Yang Ujwal Dahal Xiao-Min Lou Xi Liu Jun Huang Wei-Ping Yuan Xiao-Fan Zhu Tao Cheng Yong-Liang Zhao Xinquan Wang Jannie M Rendtlew Danielsen Feng Liu Yun-Gui Yang | 2014 | Cell Research2014，24，2： | 244 |
| 3 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 4 | Resveratrol provides neuroprotection by regulating the JAK2/STAT3/PI3K/AKT/mTOR pathway after stroke in rats显示文摘Ischemic stroke is a common disease with high mortality and morbidity worldwide.One of the important pathophysiological effects of ischemic stroke is apoptosis.A neuroprotective effect is defined as the inhibition of neuronal apoptosis to rescue or delay the infarction in the surviving ischemic penumbra.Resveratrol is a natural polyphenol that reportedly prevents cerebral ischemia injury by regulating the expression of PI3K/AKT/mTOR.Therefore,this study aimed to elucidate the neuroprotective effect of resveratrol on cerebral ischemia/reperfusion injury and to investigate the signaling pathways and mechanisms through which resveratrol regulates apoptosis in the ischemic penumbra.Rats were subjected to middle cerebral artery occlusion for 2 h followed by 24 h reperfusion.Cerebral infarct volume was measured using 2%TTC staining.TUNEL staining was conducted to evaluate neuronal apoptosis.Western blotting and immunohistochemistry were used to detect the proteins involved in the JAK2/STAT3/PI3K/AKT/mTOR pathway.The results suggested that resveratrol significantly improved neurological function,reduced cerebral infarct volume,decreased neuronal damage,and markedly attenuated neuronal apoptosis;these effects were attenuated by the inhibition of PI3K/AKT with LY294002 and JAK2/STAT3 with AG490.We also found that resveratrol significantly upregulated the expression of p-JAK2,p-STAT3,p-AKT,p-mTOR,and BCL-2 and downregulated expression of cleaved caspase-3 and BAX,which was partially reversed by LY294002 and AG490.These results suggested that resveratrol provides a neuroprotective effect against cerebral ischemia/reperfusion injury,which is partially mediated by the activation of JAK2/STAT3 and PI3K/AKT/mTOR.Resveratrol may indirectly upregulate the PI3K/AKT/mTOR pathway by activating JAK2/STAT3. | Yongying Hou Ke Wang Weijun Wan Yue Cheng Xia Pu Xiufeng Ye | 2018 | Genes & Diseases2018，5，3： | 60 |
| 5 | Efects of a bioartificial liver support system on a cetaminophen-induced acute liver failure canines显示文摘Seeinvitedcommentaryonpage286Subjectheadingsliversupportsystem;acuteliverfailure;canines;porcinehepatocytes;bioartificiallive... | XUE Yi Long 1, ZHAO Shi Feng 2, ZHANG Zuo Yun 1, WANG Yue Feng 1, LI Xin Jian 1, HUANG Xiao Qiang 3, LUO Yun 1, HUANG Ying Cai 4 and LIU Cheng Gui 1 | 1999 | World Journal of Gastroenterology1999，5，4： | 19 |
| 6 | Precise Large Deviations for Sums of Negatively Associated Random Variables with Common Dominatedly Varying Tails显示文摘在这篇论文,我们在精确大偏差上获得结果因为非鈥搑a ndom 和随机与普通 dominatedly 变化的尾巴分发功能否定地联系的 nonnegative 随机变量加。我们发现在某些条件下面,有不同集中的数字的三精确大鈥揹e viation 可能性等价于对方。而且,我们与某些否定地依赖的出现为否定地联系的 nonnegative 随机变量的和调查精确大偏差。获得的结果扩大并且改善 Ng,唐, Yan 和杨的相应结果(J。Appl。Prob, 41, 93 鈥 ? 07, 2004 ) 。否定地联系的关键词 - dominatedly 变化的尾巴 - 精确大偏差先生(2000 ) 题目分类 60F10 - 60F05 - 60G50 研究由中国(号码 10271087 ) | Yue Bao WANG Kai Yong WANG Dong Ya CHENG | 2006 | Acta Mathematica Sinica,English Series2006，22，6： | 19 |
| 7 | Neuroprotective effects of bloodletting at Jing points combined with mild induced hypothermia in acute severe traumatic brain injury显示文摘Bloodletting at Jing points has been used to treat coma in traditional Chinese medicine. Mild induced hypothermia has also been shown to have neuroprotective effects. However, the therapeutic effects of bloodletting at Jing points and mild induced hypothermia alone are limited. Therefore, we investigated whether combined treatment might have clinical effectiveness for the treatment of acute severe traumatic brain injury. Using a rat model of traumatic brain injury, combined treatment substantially alleviated cerebral edema and bloodbrain barrier dysfunction. Furthermore, neurological function was ameliorated, and cellular necrosis and the inflammatory response were lessened. These findings suggest that the combined effects of bloodletting at Jing points(20 μL, twice a day, for 2 days) and mild induced hypothermia(6 hours) are better than their individual effects alone. Their combined application may have marked neuroprotective effects in the clinical treatment of acute severe traumatic brain injury. | Yue Tu Xiao-mei Miao Tai-long Yi Xu-yi Chen Hong-tao Sun Shi-xiang Cheng Sai Zhang | 2016 | Neural Regeneration Research2016，11，6： | 17 |
| 8 | Coercivity,microstructure,and thermal stability of sintered Nd-Fe-B magnets by grain boundary diffusion with TbH3 nanoparticles显示文摘Grain boundary diffusion technique with TbH_3 nanoparticles was applied to fabricate Tb-less sintered NdFe-B permanent magnets with high coercivity. The magnetic properties and microstructure of magnets were systematically studied. The coercivity and remanence of grain boundary diffusion magnet are improved by 112% and reduced by 26% compared with those of the original magnet, respectively. Meanwhile, both the remanence temperature coefficient(α) and the coercivity temperature coefficient(β) of the magnets are improved after diffusion treatment. Microstructure shows that Tb element enriches in the surface region of Nd_2Fe_(14)B grains and is expected to exist as(Nd,Tb)_2Fe_(14)B phase. Thus, the magneto-crystalline anisotropy field of the magnet improves remarkably. As a result, the sintered Nd-FeB magnets by grain boundary diffusion with TbH_3 nanoparticles exhibit enhanced coercivity. | Wei-Qiang Liu Cheng Chang Ming Yue Jing-Shan Yang Dong-Tao Zhang Jiu-Xing Zhang Yan-Qin Liu | 2017 | Rare Metals2017，36，9： | 17 |
| 9 | Contrast-enhanced ultrasound improved performance of breast imaging reporting and data system evaluation of critical breast lesions显示文摘AIM: To determine whether contrast-enhanced ultrasound(CEUS) can improve the precision of breast imaging reporting and data system(BI-RADS) categorization. METHODS: A total of 230 patients with 235 solid breast lesions classified as BI-RADS 4 on conventional ultrasound were evaluated. CEUS was performed within one week before core needle biopsy or surgical resection and a revised BI-RADS classification was assigned based on 10 CEUS imaging characteristics. Receiver operating characteristic curve analysis was then conducted to evaluate the diagnostic performance of CEUS-based BI-RADS assignment with pathological examination as reference criteria. RESULTS: The CEUS-based BI-RADS evaluation classified 116/235(49.36%) lesions into category 3, 20(8.51%), 13(5.53%) and 12(5.11%) lesions into categories 4A, 4B and 4C, respectively, and 74(31.49%) into category 5. Selecting CEUS-based BI-RADS category 4A as an appropriate cut-off gave sensitivity and specificity values of 85.4% and 87.8%, respectively, for the diagnosisof malignant disease. The cancer-to-biopsy yield was 73.11% with CEUS-based BI-RADS 4A selected as the biopsy threshold compared with 40.85% otherwise, while the biopsy rate was only 42.13% compared with 100% otherwise. Overall, only 4.68% of invasive cancers were misdiagnosed.CONCLUSION: This pilot study suggests that evaluation of BI-RADS 4 breast lesions with CEUS results in reduced biopsy rates and increased cancer-to-biopsy yields. | Jun Luo Ji-Dong Chen Qing Chen Lin-Xian Yue Guo Zhou Cheng Lan Yi Li Chi-Hua Wu Jing-Qiao Lu | 2016 | World Journal of Radiology2016，8，6： | 17 |
| 10 | Effects of buried penis on the structure and function of corpus cavernosum in a rat model显示文摘 | CHENG Fan YU Wei-min XIA Yue ZHANG Xiao-bin YANG Si-xing GE Ming-huan | 2010 | Chinese Medical Journal2010，，13： | 17 |
| 11 | Effects of Lower Limb Alignment and Tibial Component Inclination on the Biomechanics of Lateral Compartment in Unicompartmental Knee Arthroplasty显示文摘 | Peng-Fei Wen Wan-Shou Guo Fu-Qiang Gao Qi-Dong Zhang Ju-An Yue Li-Ming Cheng Guang-Duo Zhu | 2017 | Chinese Medical Journal2017，，21： | 16 |
| 12 | Effects of long-term,low-dose sex hormone replacement therapy on hippocampus and cognition of postmenopausal women of different apoE genotypes显示文摘瞄准:学习效果长期,体积和在带 apolipoprotein E ( apoE )的绝经后的女人的马头鱼尾的怪兽的生物化学的变化上的低剂量的性荷尔蒙代替治疗( HRT )基因 epsilon 3 或 epsilon 4 .Methods :使用 HRT 的低剂量超过 4 年了的 83 个绝经后的女人作为 HRT 组被选择,并且当控制 group.ApoE 等位基因被 PCR.Magnetic 回声成像分析,有匹配的年龄和教育的 99postmenopausal 女人被注册被执行决定 brainhippocampus.Proton 的卷磁性的回声光谱学被用来在 apoE epsilon 在前面的 cingulate 外皮和马头鱼尾的怪兽检测生物化学的变化 4 并且 epsilon 3 个搬运人。六普通认知测试被用来做认知 function.Results 的全面评估: 有 4 个搬运人显示出的 apoE epsilon 的分析控制组的马头鱼尾的怪兽的卷是比 HRT 组的那些显著地低的。生物化学的分析证明有 N-acetylaspartate (NAA )/total 肌酸(tCr ) 和 myoinositol (mI ) 的减少的增加在在 HRT 的 4 个搬运人组织的 apoE epsilon 的马头鱼尾的怪兽的 /tCr,与控制组相比。为 apoEepsilon 3 个搬运人, HRT 组的最少的广场工具(LSMEAN ) 比控制 group.Conclusion 的高: 这研究证明长期的、低剂量 HRT 可能为在脆弱的绝经后的女人减少广告开发的风险是有益的。同时, HRT 能增加 apoE epsilon 的 LSMEAN 3 个搬运人。 | Yun YUE Ling HU Qin-jie TIAN Jing-mei JIANG Yi-long DONG Zheng-yu JIN yu-hang CHENG Xia HONG Qin-sheng GE Ping-ping ZUO | 2007 | Acta Pharmacologica Sinica2007，28，8： | 15 |
| 13 | Dual Phosphorylation of Suppressor of Fused (Sufu) by PKA and GSK3 beta Regulates Its Stability and Localization in the Primary Cilium显示文摘 | Chen, Yon Yue, Shen Xie, Lu Pu, Xiao-hong Jin, Tion Cheng, Steven Y. | 2011 | 南京医科大学学报(自然科学版)2011，31，6： | 15 |
| 14 | Research and Prediction of Ecological Security in Jiangsu Province Based on the Ecological Footprint显示文摘According to the 2000-2010 Jiangsu Statistical Yearbook,per capita ecological footprint and per capita ecological carrying capacity in Jiangsu Province in the years 2001-2009 are analyzed starting from the ecological footprint model.Per capita ecological deficit is measured,as well as the pressure index of ecological footprint in order to judge the grade of ecological security and to objectively evaluate the ecological security of Jiangsu Province.GM(1,1) grey forecasting model is used to forecast the ecological security of Jiangsu Province in the years 2010-2014.Research shows that in the next 5 years,both the per capita ecological footprint and the pressure index of ecological footprint will increase by 4% and 3.7% each year,respectively.And the ecological status in Jiangsu Province will be extremely unsafe.To achieve the coordinated development of ecological security and economy of Jiangsu Province,we should strictly control the population growth,rationally utilize the land resources,and strengthen the ecological restoration and construction. | CHENG Ge1,YUE Xian-ping2 1.College of Environmental Sciences,Nanjing University of Information Science & Technology,Nanjing 210044,China,2.College of Economics and Management,Nanjing University of Information Science & Technology,Nanjing 210044,China | 2011 | Asian Agricultural Research2011，3，4： | 15 |
| 15 | Mouse macrophage specific knockout of SIRT1 influences macrophage polarization and promotes angiotensin Ⅱ-induced abdominal aortic aneurysm formation显示文摘Abdominal aortic aneurysm(AAA) is a vascular degenerative disease. Macrophage polarization and the balance between classically activated macrophages(M1) and alternatively activated macrophages(M2)are crucial for AAA pathogenesis. The present study aims to investigate the roles of macrophage SIRT1 in AAA formation and macrophage polarization. We found that in mouse peritoneal macrophages, SIRT1 expression was decreased after M1 stimulation, but was enhanced after M2 stimulation. Results from SIRT1^(flox/flox) mice and macrophage specific SIRT1 knockout mice with treatment of angiotensin II(Ang II)for 4 weeks showed that macrophage specific deficiency of SIRT1 increased the incidence of AAA and exacerbated the severity, including more severe aneurysm types, enlarged diameter of the aneurysm and increased degradation of elastin. In mouse aortas, SIRT1 deficiency increased the proinflammatory M1 molecule inducible nitric oxide synthase(i NOS), and decreased M2 molecules such as arginase 1(Arg1) and mannose receptor(MR). Furthermore, in peritoneal macrophages, SIRT1 deficiency increased the expression of M1 inflammatory molecules, but decreased the expression of M2 molecules. Overexpression of SIRT1 had the opposite effects. Thus, macrophage specific knockout of SIRT1 influences macrophage polarization and accelerates Ang II-induced AAA formation. | Zhuqin Zhang Jing Xu Yue Liu Tingting Wang Jianfei Pei Liqin Cheng Delong Hao Xiang Zhao Hou-Zao Chen De-Pei Liu | 2018 | Journal of Genetics and Genomics2018，45，1： | 15 |
| 16 | The ceramide pathway is involved in the survival, apoptosis and exosome functions of human multiple myeloma cells in vitro显示文摘多重骨髓瘤(公里) 被恶意的血浆房间和倔强的同种细胞的增长描绘到传统的治疗。exosomes 涉及 modulating,这被显示出前进和通过 microRNAs (米尔) 的癌症的转移。Ceramide 是 sphingolipid 的一种类型;exosomal 分泌物的 ceramide 小径被显示了影响癌症房间的 apoptosis。但是在公里房间功能, exosome 功能和米尔规定的这条小径的角色仍然保持未知。在这研究,我们显示出那 C6 ceramide (外长的 ceramide 补充, 1.25-40 mol/L ) dose-dependently 禁止了增长并且在人的公里 OPM2 房间线支持了 apoptosis,它与提高的 caspase 3/9 和 PARP 劈开被联系。我们也发现 C6 ceramide (5-20 mol/L ) dose-dependently 刺激了 exosome 分泌物并且增加了肿瘤镇压的米尔的 exosomal 层次(米尔 202,米尔 16,米尔 29b 和米尔 15a ) 。笔记,从 C6 对待 ceramide 的 OPM2 房间的 exosomes 能影响接受者 OPM2 房间的增长和 apoptosis,它与增加的肿瘤镇压的 exosomal 米尔相关。相反, GW4869 (一个 ceramide 禁止者, 5-20 mol/L ) 在公里 exosomes 在公里功能, exosome 分泌物和米尔层次的规定上施加了相反的效果。然而,从对待 GW4869 的 OPM2 房间的 exosomes 没在这些米尔和指向的 OPM2 房间的幸存上有效果。一起拿,我们的调查结果表明 ceramide 小径调制公里幸存,可能直接经由 caspase 小径并且间接地经由 exosomal 米尔机制。 | Qian CHENG Xin LI Yue WANG Min DONG Feng-huang ZHAN Jing LIU | 2018 | Acta Pharmacologica Sinica2018，39，4： | 15 |
| 17 | Rapid report of the 8 January 2022 M_(S)6.9 Menyuan earthquake,Qinghai,China显示文摘The M_(S)6.9 Menyuan earthquake in Qinghai Province,west China is the largest earthquake by far in 2022.The earthquake occurs in a tectonically active region,with a background b-value of 0.87 within 100 km of the epicenter that we derived from the unified catalog produced by China Earthquake Networks Center since late 2008.Field surveys have revealed surface ruptures extending 22 km along strike,with a maximum ground displacement of 2.1 m.We construct a finite fault model with constraints from In SAR observations,which showed multiple fault segments during the Menyuan earthquake.The major slip asperity is confined within 10 km at depth,with the maximum slip of 3.5 m.Near real-time back-projection results of coseismic radiation indicate a northwest propagating rupture that lasted for~10 s.Intensity estimates from the back-projection results show up to a Mercalli scale of IX near the ruptured area,consistent with instrumental measurements and the observations from the field surveys.Aftershock locations(up to January 21,2022)exhibit two segments,extending to~20 km in depth.The largest one reaches M_(S)5.3,locating near the eastern end of the aftershock zone.Although the location and the approximate magnitude of the mainshock had been indicated by previous studies based on paleoearthquake records and seismic gap,as well as estimated stressing rate on faults,significant surfacebreaching rupture leads to severe damage of the high-speed railway system,which poses a challenge in accurately assessing earthquake hazards and risks,and thus demands further investigations of the rupture behaviors for crustal earthquakes. | Hongfeng Yang Dun Wang Rumeng Guo Mengyu Xie Yang Zang Yue Wang Qiang Yao Chuang Cheng Yanru An Yingying Zhang | 2022 | Earthquake Research Advances2022，2，1： | 14 |
| 18 | Antigen-specific tolerance induced by IL-10 gene modified immature dendritic cells in experimental autoimmune myocarditis in rats显示文摘Background Experimental autoimmune myocarditis (EAM) in rats is a T-cell-mediated disorder. The initiation and maintenance of autoimmune responses in EAM depend on the maturation state of dendritic cells. IL-10 is a pleiotrophic immunomodulatory cytokine that functions at different levels of the immune response, so it has emerged as a promising therapeutic factor for the treatment of autoimmune/inflammatory diseases. This study was designed to test the hypothesis that IL-10 gene modified bone marrow-derived immature dendritic cells (iDCs) ameliorate EAM and to explore the underlying mechanisms. Methods EAM was induced using the methods of cardiac myosin immunization on day 0 and day 7. Immature and mature bone marrow-derived dendritic cells (BMDCs) were generated without or with the stimulation by lipopolysaccharide (LPS) and the phenotype was analyzed by flow cytometry. Some of the iDCs were transfected by pcDNA3-IL-10 plasmid. 2×106/per rat mature DC (mDC), immature DC (iDC), pcDNA3 transfected iDC, pcDNA3-IL-10 transfected iDC or phosphate buffered saline (PBS) were injected intravenously for treatment 5 days after the first immunization. On day 21, HE staining was performed to detect the myocardial inflammation and T lymphocyte proliferation assay was used to determine the effects of IL-10 gene transfected iDC on autoreactive T cell proliferation. Expression of IκB, the inhibitor of NF-κB pathway, was determined by Western blot. Results BMDCs generated in a medium supplemented with granulocyte-macrophage-colony-stimulating factor (GM-CSF) were relatively immature, as determined by flow cytometry. However, stimulation with LPS induced these cells to become mature (m)DCs with higher levels of surface major histocompatibility complex (MHC)-II and costimulatory molecules. Intravenous administration of iDCs, especially pcDNA3-IL-10 transfected iDC, ameliorated the histopathological severity of the myosin induced-EAM, and the effect was lost after the DCs underwent maturation induced by in vitro exposure to LPS. IL-10 gene modified iDC inhibited the antigen specific T cell responses towards cardiac myosin. IκB protein was up-regulated significantly in the IL-10 gene modified iDC group. Conclusions IL-10 gene modified iDC induced antigen-specific tolerance in EAM. The underlying mechanisms may be related to costimulatory molecules down-regulation and NF-κB pathway inhibition. | LI Wei-min LIU Wei GAO Cheng ZHOU Bao-guo YANG Shu-sen WANG Zheng ZHANG Rui-hong GAN Run-tao KONG Yi-hui LI Yue | 2006 | Chinese Medical Journal2006，，19： | 13 |
| 19 | The interplay of host genetic factors and Epstein-Barr virus in the development of nasopharyngeal carcinoma显示文摘The interplay between host cell genetics and Epstein-Barr virus(EBV) infection contributes to the development of nasopharyngeal carcinoma(NPC). Understanding the host genetic and epigenetic alterations and the influence of EBV on cell signaling and host gene regulation will aid in understanding the molecular pathogenesis of NPC and provide useful biomarkers and targets for diagnosis and therapy. In this review, we provide an update of the oncogenes and tumor suppressor genes associated with NPC, as well as genes associated with NPC risk including those involved in carcinogen detoxification and DNA repair. We also describe the importance of host genetics that govern the human leukocyte antigen(HLA) complex and immune responses, and we describe the impact of EBV infection on host cell signaling changes and epigenetic regulation of gene expression. High-power genomic sequencing approaches are needed to elucidate the genetic basis for inherited susceptibility to NPC and to identify the genes and pathways driving its molecular pathogenesis. | Maria Li Lung Arthur Kwok Leung Cheung Josephine Mun Yee Ko Hong Lok Lung Yue Cheng Wei Dai | 2014 | Chinese Journal of Cancer2014，33，11： | 13 |
| 20 | Expression of lens-related microRNAs in transparent infant lenses and congenital cataract显示文摘AIM:To identify the expression of lens-related micro RNAs(miRNAs)in the central epithelium of transparent infant lenses and congenital cataract. METHODS:Lens-related mi RNAs were retrieved from Pub Med database. The expression levels of these mi RNAs in transparent infant lenses and congenital cataract were determined by stem-loop reverse transcription-polymerase chain reaction(RT-PCR). mi Randa algorithm was used to predict the target genes of these differentially expressed mi RNAs. The target m RNA was validated.RESULTS:Six lens-related mi RNAs were retrieved from screening Pub Med database. The most abundant mi RNA in transparent infant lenses according to stem-loop RT-PCR was mi R-184. miR-182 was up-regulated in congenital cataract. Contrarily,miR-204 and miR-124 was down-regulated.mi R-204 exhibited a more significant decrease in expression than mi R-124. In addition,Meis2 was predicted to be the target of mi R-204 using mi Randa algorithm. mi R-204mimic/antagomir transfection experiments suggested the negative correlation between the expression of mi R-204 and Meis2.CONCLUSION:The expression levels of miR-182,miR-204 and mi R-124 differ between the central epithelium of transparent infant lens and congenital cataract,suggesting their involvement in the pathogenesis of congenital cataract. miR-204 may act via silencing Meis2 to regulate lens development and congenital cataract formation. | Chang-Rui Wu Min Ye Li Qin Yue Yin Cheng Pei | 2017 | International Journal of Ophthalmology(English edition)2017，10，3： | 11 |
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