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| 1 | Double role of Fas ligand in the apoptosis of intervertebral disc cells in vitro显示文摘船边交货 ligand (FasL ) 可以在维持 intervertebral 圆盘(IVD ) 的有免疫力的特权起一个重要作用。而且,它是仔细与退化圆盘房间的 apoptosis 有关。现在,大量报告关于 FasL 的矛盾的效果描述了,尽管 IVD 房间上的 FasL 的效果仍然在争论下面。在这研究,我们试着在船边交货表示上并且在原子核 pulposus (NP ) 的 apoptosis 上调查 FasL 的效果在 SpragueDawley 老鼠的房间。结果证明在 NP 房间的船边交货的表示被 recombinant FasL 显著地增加。同时, NP 房间的 apoptosis 以一种 FasL 剂量依赖者方式显著地增加了。有趣地, RNA 干扰结果显示船边交货表示和以前描述的 NP 房间 apoptosis 的增加被船边交货 siRNA 禁止,建议 RNA 干扰可能是阻止 IVD 房间 apoptosis 的新奇策略之一。 | Dunfu Han Yue Ding Shang-Li Liu Gang Wang In-Cheong Si Xinguang Wang Liyang Cui Dongshang Huang | 2009 | Acta Biochimica et Biophysica Sinica2009，41，11： | 14 |
| 2 | Anlotinib has good efficacy and low toxicity: a phase Ⅱ study of anlotinib in pre-treated HER-2 negative metastatic breast cancer显示文摘Objective:Anlotinib is a novel tyrosine kinase inhibitor blocking angiogenesis.This study was performed to assess the efficacy and safety of anlotinib in patients with metastatic breast cancer.Methods:Patients with HER2-negative breast cancer,who were pre-treated with anthracycline or taxanes in a neoadjuvant,adjuvant,or metastatic setting,and had treatment failure after at least one prior chemotherapy regimen in the metastatic setting were enrolled.Anlotinib was administered at 12 mg daily for 14 days in a 21-day cycle until disease progression or unacceptable toxicity occurred.Simultaneously,5–10 m L of venous blood was collected to perform circulating tumor DNA(ct DNA)testing every 2 treatment cycles.The primary endpoint was the objective response rate(ORR).Secondary endpoints included the disease control rate(DCR),progression-free survival(PFS),overall survival,safety,and biomarkers.Results:Twenty-six eligible patients were enrolled,with a median age of 56(30–75)years.The median follow-up time was 10.5 months.The ORR was 15.4%,the DCR was 80.8%,and the median PFS was 5.22 months(95%confidence interval 2.86–6.24).Fourteen(53.8%)patients survived for more than 10 months.The changes in the detectable ct DNA variant allele frequency were consistent with the tumor response.The most common treatment-related adverse events were hypertension(57.7%),thyroidstimulating hormone elevation(34.6%),and hand-foot syndrome(23.1%).Conclusion:Anlotinib showed objective efficacy with tolerable toxicity in heavily pre-treated,metastatic HER2-negative breast cancer.The dynamic changes in the ct DNA variant allele fraction may be predictive of the tumor response. | Nanlin Hu Yiran Si Jian Yue Tingting Sun Xue Wang Zhuqing Jia Songlin Gao Qiao Li Yang Shao Jiayu Wang Yang Luo Fei Ma Binghe Xu Peng Yuan | 2021 | Cancer Biology & Medicine2021，18，3： | 11 |
| 3 | Recursive super-convergence computation for multi-dimensional problems via one-dimensional element energy pro jection technique显示文摘This paper presents a strategy for computation of super-convergent solutions of multi-dimensional problems in the finite element method(FEM) by recursive application of the one-dimensional(1D) element energy projection(EEP) technique. The main idea is to conceptually treat multi-dimensional problems as generalized 1D problems,based on which the concepts of generalized 1D FEM and its consequent EEP formulae have been developed in a unified manner. Equipped with these concepts, multi-dimensional problems can be recursively discretized in one dimension at each step, until a fully discretized standard finite element(FE) model is reached. This conceptual dimension-bydimension(D-by-D) discretization procedure is entirely equivalent to a full FE discretization. As a reverseD-by-D recovery procedure, by using the unified EEP formulae together with proper extraction of the generalized nodal solutions, super-convergent displacements and first derivatives for two-dimensional(2D) and three-dimensional(3D) problems can be obtained over the domain. Numerical examples of 3D Poisson's equation and elasticity problem are given to verify the feasibility and effectiveness of the proposed strategy. | Si YUAN Yue WU Qinyan XING | 2018 | Applied Mathematics and Mechanics(English Edition)2018，39，7： | 11 |
| 4 | Spinal Cord Stimulation Frequency Influences the Hemodynamic Response in Patients with Disorders of Consciousness显示文摘Spinal cord stimulation(SCS) is a promising technique for treating disorders of consciousness(DOCs).However, differences in the spatio-temporal responsiveness of the brain under varied SCS parameters remain unclear. In this pilot study, functional near-infrared spectroscopy was used to measure the hemodynamic responses of 10 DOC patients to different SCS frequencies(5 Hz, 10 Hz, 50 Hz, 70 Hz, and 100 Hz). In the prefrontal cortex, a key area in consciousness circuits, we found significantly increased hemodynamic responses at 70 Hz and 100 Hz, and significantly different hemodynamic responses between 50 Hz and 70 Hz/100 Hz. In addition, the functional connectivity between prefrontal and occipital areas was significantly improved with SCS at 70 Hz. These results demonstrated that SCS modulates the hemodynamic responses and long-range connectivity in a frequency-specific manner(with 70 Hz apparently better), perhaps by improving the cerebral blood volume and information transmission through the reticular formation-thalamus-cortex pathway. | Juanning Si Yuanyuan Dang Yujin Zhang Yaxin Li Wenyue Zhang Yi Yang Yue Cui Xiaoping Lou Jianghong He Tianzi Jiang | 2018 | Neuroscience Bulletin2018，34，4： | 10 |
| 5 | The Status and Associated Factors of Successful Aging among Older Adults Residing in Longevity Areas in China显示文摘Objective This study aims to assess the status of successful aging(SA) in longevity areas in China and explore multiple factors associated with SA among the young-old and oldest-old. Methods A total of 2296 elderly people aged 65 and older were interviewed in the longevity areas sub-sample of the Chinese Longitudinal Healthy Longevity Survey(CLHLS) in 2012. Baseline assessments included a researcher-administered questionnaire, physical examination, and laboratory testing. A logistic regression model was used to identify factors associated with SA. Results The prevalence of SA was 38.81% in the CLHLS in 2012. There were significant differences between ages groups, with SA compromising 56.85% among ≥65 years group and 20.31% among ≥100 years group(χtrend2=126.73, P<0.01). The prevalence of SA among females was 33.59%, which was significantly lower than that among males(45.58%)(χ2gender=33.65, P<0.05). In the regression analysis, having anemia(OR=0.744, 95% CI: 0.609-0.910), poor lifestyle(OR=0.697, 95% CI: 0.568-0.854), poor sleep quality(OR=0.558, 95% CI: 0.456-0.682), and central obesity(OR=0.684, 95% CI: 0.556-0.841) were the main factors associated with SA. The promoting SA rate decreased as age increased, and the group of 65-79 years had higher odds than the other age group. Conclusion Preventing central obesity, improving sleep quality and promoting healthy lifestyle may contribute to achieve SA among the elderly. | SHI Wen Hui ZHANG Hong Yan ZHANG Juan LYU Yue Bin Melanie Sereny Brasher YIN Zhao Xue LUO Jie Si HU Dong Sheng FEN Lei SHI Xiao Ming | 2016 | Biomedical and Environmental Sciences2016，29，5： | 10 |
| 6 | Review of control and guidance technology on hypersonic vehicle显示文摘Air-breathing hypersonic vehicle has great military and potential economic value due to its characteristics:high velocity,long range,quick response.Therefore,the development of hypersonic vehicle and its guidance and control technology are reviewed in this paper.Firstly,the development and classification of hypersonic vehicles around the world are summarized,and the geometric configuration and mission profile of typical air-breathing hypersonic vehicle are given.Secondly,the control difficulties of air-breathing hypersonic vehicle are introduced,including integrated design of engine and fuselage,static instability,strong nonlinearity,uncertain aerodynamic parameters,etc.According to its control requirements,the control methods considering external disturbance,fault-tolerant control methods,anti-saturation methods,and prescribed performance control methods considering transient performance constraints are summarized respectively.The classification and comparison of various control methods are given,and the frontiers of theoretical development are analyzed.Finally,considering the effects of composite disturbances,the design of terminal guidance law under multiple constraints is overviewed,including guidance law with angle constraint,velocity constraint,acceleration constraint and time constraint.Similarly,the classification of guidance law design methods under different constraints,their advantages as well as the future development trend and requirements are introduced. | Yibo DING Xiaokui YUE Guangshan CHEN Jiashun SI | 2022 | Chinese Journal of Aeronautics2022，35，7： | 9 |
| 7 | Clean Energy Consumption of Power Systems Towards Smart Agriculture: Roadmap, Bottlenecks and Technologies显示文摘Over the past decades,both agriculture and power systems have faced serious problems,such as the power supply shortage in agriculture,and difficulties of clean energy consump-tion in the power system.To address and overcome these issues,this paper proposes an idea to combine smart agriculture and clean energy consumption,use surplus clean energy to supply agriculture production,and utilize smart agriculture to support power system with clean energy penetration.A comprehensive review has been conducted to first depict the roadmap of coupling a agriculture-clean energy system,analyze their feasibilities and advantages.The recent technologies and bottlenecks are summa-rized and evaluated for the development of a combined system consisting of smart agriculture production and clean energy consumption.Several case studies are introduced to explore the mutual benefits of agriculture-clean energy systems in both the energy and food industries. | Junyong Liu Yanxin Chai Yue Xiang Xin Zhang Si Gou Youbo Liu | 2018 | CSEE Journal of Power and Energy Systems2018，4，3： | 9 |
| 8 | Diallyl trisulfide induces apoptosis and inhibits proliferation of A549 cells in vitro and in vivo显示文摘肺癌症是在整个世界的癌症相关的死亡的领先的原因。在最近的年里,肺的腺癌在频率超过了有鳞的房间癌并且是在许多国家的肺癌症的占优势的形式。流行病学的调查显示出在消费和死亡从许多癌症评估的大蒜(瞀或蒜之类 sativum ) 之间的一种反的关系。Diallyl trisulfide (DATS ) 是导出大蒜的混合物之一(是也知道:organosulfer 混合物,振荡) 。DATS 能导致 apoptosis 并且禁止许多癌症细胞线的生长。我们的学习证明 DATS 导致的 apoptotic 事件是通过导致了 Bax/Bcl-2 和 caspase-3, -8, 和 -9 的活动的比率的起来规定的 anti-apoptotic Bcl-2 的重要减少的线粒体依赖者 caspase 串联。最后, DATS 导致了 apoptosis 并且在一个集中依赖者和时间依赖者举止禁止了增长。由与 A549 异种皮移植建立女 BALB/c 裸体老鼠的一个动物模特儿,没有与控制组相比引起重量损失或任何另外的副作用,而且,我们在裸体老鼠发现 DATS 的那口头的 gavage A549 异种皮移植的显著地延迟的生长。在 vitro 并且在 vivo 的所有证据建议 DATS 能是理想的反癌症药。 | Wenjun Li Hui Tian Lin Li Shuhai Li Weiming Yue Zhitao Chen Lei Qi Wensi Hu Yingchao Zhu Bin Hao Cun Gao Libo Si Fei Gao | 2012 | Acta Biochimica et Biophysica Sinica2012，44，7： | 6 |
| 9 | Large-area growth of ultra-high-density single-walled carbon nanotube arrays on sapphire surface显示文摘获得高密度的、大区域的围单人赛的碳 nanotube (SWNT ) 的可伸缩的途径穿的 A 为在实际电子设备认识到 SWNT 的完整的潜力是必要的;这仍然是大挑战。这里,我们由联合特洛伊催化剂在蓝宝石表面上为 ultra-high-density SWNT 数组的大区域的生长报导一个改进合成方法(免除了底层,保证超离频密度) 与瞬间 nanoparticles (在表面上装载了,稳定释放特洛伊催化剂) 作为合作催化剂。稠密、非常排列的 SWNT 盖住全部底层,本地密度象 160 tubes/m 一样高。在如此的数组上造的地效果晶体管(联邦货物税) 在排水管来源电压给了 488 A/m 的产量电流密度(V ds )= 门来源电压(V gs )=2 V,相应于在传导力上每 244 S/m 的宽度。这些结果在 SWNT 的控制结构的生长证实特洛伊瞬间催化剂的潜在的应用的宽范围。 | Lixing Kang Yue Hu Hua Zhong Jia Si Shuchen Zhang Qiuchen Zhao Jingjing Lin Qingwen Li Zhiyong Zhang Lianmao Peng Jin Zhang | 2015 | Nano Research2015，8，11： | 5 |
| 10 | Optimizing the crystallinity and acidity of H-SAPO-34 by fluoride for synthesizing Cu/SAPO-34 NH_3-SCR catalyst显示文摘A series of H-SAPO-34 zeolites were synthesized by a hydrothermal method in fluoride media.The as-synthesized H-SAPO-34 zeolites were characterized by X-ray diffraction(XRD),scanning electron microscopy(SEM),N_2 physisorption,temperature-programmed desorption of NH_3(NH_3-TPD) and nuclear magnetic resonance(NMR) measurements.The results showed that a certain concentration of F- anions promoted the nucleation and crystallization of H-SAPO-34.The H-SAPO-34 synthesized in the fluoride media showed high crystallinity,uniform particle size distribution,large specific surface area and pore volume,and enhanced acidity.Therefore,Cu/SAPO-34 based on the fluoride-assisted zeolite showed a broadened temperature window for the selective catalytic reduction of NO by NH_3(NH_3-SCR) reaction due to the enhanced acidity of the zeolite and the improved dispersion of copper species. | Jing Ma Zhichun Si Xiaodong Wu Duan Weng Yue Ma | 2016 | Journal of Environmental Sciences2016，28，3： | 5 |
| 11 | One-Step High-Temperature-Synthesized Single-Atom Platinum Catalyst for Efficient Selective Hydrogenation显示文摘Although single-atom catalysts significantly improve the atom utilization efficiency,the multistep preparation procedures are complicated and difficult to control.Herein,we demonstrate that one-step in situ synthesis of the single-atom Pt anchored in single-crystal MoC(Pt_(1)/MoC)by using facile and controllable arc-discharge strategy under extreme conditions.The high temperature(up to 4000℃)provides the sufficient energy for atom dispersion and overall stability by forming thermodynamically favourable metal-support interactions.The high-temperature-stabilized Pt1/MoC exhibits outstanding performance and excellent thermal stability as durable catalyst for selective quinoline hydrogenation.The initial turnover frequency of 3710 h^(-1)is greater than those of previously reported samples by an order of magnitude under 2 MPa H_(2)at 100℃.The catalyst also shows broad scope activity toward hydrogenation containing unsaturated groups of C=C,C=N,and C=O.The facile,one-step,and fast arc-discharge method provides an effective avenue for single-atom catalyst fabrication that is conventionally challenging. | Qingyuan Bi Xiaotao Yuan Yue Lu Dong Wang Jian Huang Rui Si Manling Sui Fuqiang Huang | 2020 | Research2020，，1： | 5 |
| 12 | The influence of various frequency chorus waves on electron dynamics in radiation belts显示文摘Non-adiabatic behaviour induced by the chorus-electron interaction is an important contributor to the radiation belt dynamics,and largely relies on wave frequency distribution.During the geomagnetic storm on August 2,2016,upper-band,lower-band and extremely low frequency(ELF)chorus waves were simultaneously observed within one orbit period of the Van Allen Probe B.Numerical simulations are performed to investigate the electron evolution by the observed chorus with different frequencies.The results show that various frequency chorus waves have different effects on electron dynamics.For chorus in the range f≈0.3 fce–0.7 fce,energy diffusion is the dominant process in electron evolutions.For chorus in the range 0.1 fce≤f≤0.25 fce,the pitch angle diffusion tends to be comparable to energy diffusion for Ek>0.5 MeV.For ELF chorus below 0.1 fce,the pitch angle diffusion rate is much above the energy diffusion rate,leading to potential scattering losses. | HE JiaBei JIN Yu Yue XIAO FuLiang HE ZhaoGuo YANG Chang XIE YanQiong HE Qian WANG ChengZhi SHANG XiongJun LIU Si ZHOU QingHua ZHANG Sai | 2021 | Science China(Technological Sciences)2021，64，4： | 3 |
| 13 | Short-term Chronic Intermittent Hypobaric Hypoxia Alters Gut Microbiota Composition in Rats显示文摘Chronic intermittent hypobaric hypoxia (CIHH) is a treatment of moderate hypoxia that simulates high altitude interrupted by normoxia. Growing evidence shows that CIHH has multiple beneficial effects on the body[1]. Our previous studies showed that CIHH (simulating 5, 000-m altitude for 28 days, 6 hours daily) protects the heart against ischemic/reperfusion-induced arrhythmias and damage through increased coronary flow and myocardial capillary angiogenesis, enhanced cardiac anti-oxidation, induction of heat shock protein 70, inhibition of endoplasmic reticulum stress, activation of ATP-sensitive potassium channels, and inhibition of mitochondrial permeability transition pores[2]. In addition, CIHH decreases high blood pressure by facilitating the baroreceptor reflex and enhancing arterial relaxation in renal vascular hypertensive rats. CIHH also improves metabolic dysfunction in rats with diabetes and metabolic syndrome. However, the mechanism is not fully understood. | TIAN Yan Ming GUAN Yue TIAN Si Yu YUAN Fang ZHANG Li ZHANG Yi | 2018 | Biomedical and Environmental Sciences2018，31，12： | 3 |
| 14 | Factors associated with upstaging in patients preoperatively diagnosed with ductal carcinoma in situ by core needle biopsy显示文摘Objective: Patients preoperatively diagnosed with ductal carcinoma in situ(DCIS) by core needle biopsy(CNB) exhibit a significant risk for upstaging on final pathology, which leads to major concerns of whether axillary staging is required at the primary operation. The present study aimed to identify clinicopathological factors associated with upstaging in patients preoperatively diagnosed with DCIS by CNB.Methods: The present study enrolled 604 patients(cN0 M0) with a preoperative diagnosis of pure DCIS by CNB, who underwent axillary staging between August 2006 and December 2015, at Fudan University Shanghai Cancer Center(Shanghai, China).Predictive factors of upstaging were analyzed retrospectively.Results: Of the 604 patients, 20.03%(n = 121) and 31.95%(n = 193) were upstaged to DCIS with microinvasion(DCISM) and invasive breast cancer(IBC) on final pathology, respectively. Larger tumor size on ultrasonography(> 2 cm) was independently associated with upstaging [odds ratio(OR) 1.558, P = 0.014]. Additionally, patients in lower breast imaging reporting and data system(BI-RADS) categories were less likely to be upstaged(4 B vs. 5: OR 0.435, P = 0.002;4 C vs. 5: OR 0.502, P = 0.001). Overall,axillary metastasis occurred in 6.79%(n = 41) of patients. Among patients with axillary metastasis, 1.38%(4/290), 3.31%(4/121)and 17.10%(33/193) were in the DCIS, DCISM, and IBC groups, respectively.Conclusions: For patients initially diagnosed with DCIS by CNB, larger tumor size on ultrasonography(> 2 cm) and higher BIRADS category were independent predictive factors of upstaging on final pathology. Thus, axillary staging in patients with smaller tumor sizes and lower BI-RADS category may be omitted, with little downstream risk for upstaging. | Jing Si Benlong Yang Rong Guo Naisi Huang Chenlian Quan Linxiaoxi Ma Bingqiu Xiu Yun Cao Yue Tang Linxiao Shen Jiajian Chen Jiong Wu | 2019 | Cancer Biology & Medicine2019，16，2： | 3 |
| 15 | D e novo hepatitis B virus infection from anti‐HBc‐positive donors in pediatric living donor liver transplantation显示文摘 | Zhi Feng Xi Qiang Xia Jian Jun Zhang Xiao Song Chen Long Zhi Han Jian Jun Zhu Si Yue Wang De Kai Qiu | 2013 | Journal of Digestive Diseases2013，，8： | 2 |
| 16 | The preliminary processing and analysis of LPR Channel-2B data from Chang'E-3显示文摘The Lunar Penetrating Radar(LPR)carried by Chang’E-3 has imaged the shallow subsurface of the landing site at the northern Mare Imbrium.The antenna B of the Channel-2 onboard the LPR(LPR Channel-2B)has collected more than 2000 traces of usable raw data.Because of the low resolution and noise of the raw data,only a few shallow geological structures are visible.To improve the resolution and the signal-to-noise ratio of the LPR data,we processed the LPR data including amplitude compensation,filtering,and deconvolution processes.The processing results reveal that the data processing in this study not only improves the signal-to-noise ratio of the LPR Channel-2B data but also makes the geological structures vivid.The processing results will lay the foundation for the subsequent geological interpretation and physical property inversion of lunar materials. | ZHAO Na ZHU Pei Min YANG Ke Si YUAN Yue Feng GUO Shi Li | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，12： | 2 |
| 17 | Trend and seasonal variations of atmospheric CH_4 in Beijing显示文摘The atmospheric CH\-4 in Beijing is still increasing, even though its increasing rate has significantly decreased from 1.76%/a during 1985—1989 to 0.50%/a during 1990—1997. The seasonal variation of CH\-4 concentration showed a double\|peak pattern, one peak appearing in winter and the other in summer. It is evident that the annually seasonal variations of atmospheric CH\-4 in Beijing are different. From 1986 to 1997, the atmospheric CH\-4 increased by 185 ppbv, 37% and 21% of which were due to the increase in winter and in summer, respectively. After 1993, the annually seasonal increasing rate of CH\-4 concentration in summer (due to emission from biogenic sources) is negative while the increasing rate in winter (due to emission from non\|biogenic sources) is positive about 25 ppbv/a. As a result, the increase of CH\-4 emission from non\|biogenic sources in winter is the major reason that caused the annually seasonal increasing rate from 1993 to 1997. The biogenic sources in Beijing are shrinking while the non\|biogenic ones (such as fossil fuel combustion) are enlarging. | WANG Yue\|si, WANG Ming\|xing, LUO Dong\|mei, ZHENG Xun\|hua, ZHOU Li (Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China) | 2000 | Journal of Environmental Sciences2000，12，3： | 2 |
| 18 | Down-regulation of MTA1 protein leads to the inhibition of migration, invasion, and angiogenesis of non-small-cell lung cancer cell line显示文摘联系转移的蛋白质 1 (MTA1 ) 高表示在许多人的好攻击的肿瘤被检测了并且在象侵略,转移,和 angiogenesis 那样的恶意的生物行为起重要作用。然而,在调整 non-small-cell 肺癌症(NSCLC ) 的恶意的行为的 MTA1 蛋白质的特定的角色和机制房间仍然保持不清楚。阐明 MTA1 蛋白质的详细函数,我们下面调整在由在 vitro 的 RNA 干扰(RNAi ) 的 NSCLC 房间线的 MTA1 蛋白质表达式,并且发现 MTA1 蛋白质的下面规定显著地禁止了 95D 房间的移植和侵略潜力。进一步的研究表明 MTA1 蛋白质的下面规定显著地减少了矩阵 metalloproteinase-9 的活动,它能是为 95D 房间移植和侵略的抑制负责的机制。另外,试管形成试金证明 MTA1-siRNA 95D 房间的调节媒介导致的完全的试管的数字比 95D 房间的显著地小。这些调查结果证明那 MTA1 蛋白质在调整 95D 房间的迁居,侵略,和 angiogenesis 潜力起重要作用,由 RNAi 建议那条 MTA1 蛋白质下面规定可能是一条新奇治疗学的途径禁止 NSCLC 的前进。 | Shuhai Li Hui Tian Weiming Yue Lin Li Cun Gao Libo Si Wenjun Li Wensi Hu Lei Qi Ming Lu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，2： | 2 |
| 19 | Construction of Salmonella Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific-pathogen-free chicks显示文摘In this study,a safety enhanced Salmonella Pullorum(S.Pullorum)ghost was constructed using an antimicrobial peptide gene,and evaluated for its potential as a Pullorum disease(PD)vaccine candidate.The antimicrobial peptide SMAP29 was co-expressed with lysis gene E to generate S.Pullorum ghosts.No viable bacteria were detectable either in the fermentation culture after induction of gene E-and SMAP29-mediated lysis for 24 h or in the lyophilized ghost products.Specific-pathogenfree(SPF)chicks were intraperitoneally immunized with ghosts at day 7 of age and no mortality,clinical symptoms or signs of PD such as anorexia,depression and diarrhea were observed.On challenge with a virulent S.Pullorum strain at 4 wk post-immunization,a comparatively higher level of protection was observed in the S.Pullorum ghost immunized chickens with a minimum of pathological lesions and bacterial loads compared to the birds in inactivated vaccine groups.In addition,immunization with the S.Pullorum ghosts induced a potent systemic Ig G response and was associated with significantly increased levels of cytokine IFN-γand IL-4 and relative percentages of CD4^+ and CD8^+ T lymphocytes.Our results indicate that SMAP29 can be employed as a new secondary lethal protein to enhance the safety of bacterial ghosts,and to prepare a non-living bacterial vaccine candidate that can prevent PD in chickens. | TIAN Qiu-feng ZHOU Wei SI Wei YI Fei HUA Xin YUE Min CHEN Li-ping LIU Si-guo YU Shen-ye | 2018 | Journal of Integrative Agriculture2018，17，1： | 2 |
| 20 | A Novel ATM Antisense Transcript ATM-AS Positively Regulates ATM Expression in Normal and Breast Cancer Cells显示文摘Objective:The ataxia telangiectasia mutated(ATM)gene is a master regulator in cellular DNA damage response.The dysregulation of ATM expression is frequent in breast cancer,and is known to be involved in the carcinogenesis and prognosis of cancer.However,the underlying mechanism remains unclear.The bioinformatic analysis predicted a potential antisense transcript ATM-antisense(AS)from the opposite strand of the ATM gene.The purpose of this study was to identify ATM-AS and investigate the possible effect of ATM-AS on the ATM gene regulation.Methods:Single strand-specific RT-PCR was performed to verify the predicted antisense transcript ATM-AS within the ATM gene locus.qRT-PCR and Western blotting were used to detect the expression levels of ATM-AS and ATM in normal and breast cancer cell lines as well as in tissue samples.Luciferase reporter gene assays,biological mass spectrometry,ChIP-qPCR and RIP were used to explore the function of ATM-AS in regulating the ATM expression.Immunofluorescence and host-cell reactivation(HCR)assay were performed to evaluate the biological significance of ATM-AS in ATM-mediated DNA damage repair.Breast cancer tissue samples were used for evaluating the correlation of the ATM-AS level with the ATM expression as well as prognosis of the patients.Results:The ATM-AS significantly upregulated the ATM gene activity by recruiting KAT5 histone acetyltransferase to the gene promoter.The reduced ATM-AS level led to the abnormal downregulation of ATM expression,and impaired the ATM-mediated DNA damage repair in normal breast cells in vitro.The ATM-AS level was positively correlated with the ATM expression in the examined breast cancer tissue samples,and the patient prognosis.Conclusion:The present study demonstrated that ATM-AS,an antisense transcript located within the ATM gene body,is an essential positive regulator of ATM expression,and functions by mediating the binding of KAT5 to the ATM promoter.These findings uncover the novel mechanism underlying the dysregulation of the ATM gene in breast cancer,and enrich our understanding of how an antisense transcript regulates its host gene. | He CHENG Er-shao ZHANG Xiao SHI Ping-ping CAO Bei-jing PAN Xin-xin SI Yue LIU Nan YANG Ying CHU Xu-chun WANG Xiao HAN Zhi-hong ZHANG Yu-jie SUN | 2022 | Current Medical Science2022，42，4： | 2 |
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