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| 1 | Characterization and source analysis of indoor/outdoor culturable airborne bacteria in a municipal wastewater treatment plant显示文摘The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor(i.e., fine screen room and sludge dewatering house) and two outdoor(i.e., aeration tank and control site) sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely,Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria. | Guangsu Xu Yunping Han Lin Li Junxin Liu | 2018 | Journal of Environmental Sciences2018，30，12： | 12 |
| 2 | Characterization of the airborne bacteria community at different distances from the rotating brushes in a wastewater treatment plant by 16S rRNA gene clone libraries显示文摘Biological risks of bioaerosols emitted from wastewater treatment processes have attracted wide attention in the recent years. However, the culture-based analysis method has been mostly adopted for detecting the bacterial community in bioaerosols, which may result in the underestimation of total microorganism concentration as not all microorganisms are cultivable. In this study, oligonucleotide fingerprinting of 16S rRNA genes was applied to reveal the composition and structure of the bacterial community in bioaerosols from an Orbal oxidation ditch in a Beijing wastewater treatment plant (WWTP). Bioaerosols were collected at different distances from the aerosol source, rotating brushes, and the sampling height was 1.5 m which is the common respiratory height of a human being. The bacterial communities of bioaerosols were diverse, and the lowest bacterial diversity was found at the sampling site just after the rotating brush rotating brush. A large proportion of bacteria in bioaerosols were affiliated with Proteobacteria and Bacteroidetes. Numerous bacteria present in the bioaerosols also emerged in water, indicating that the bacterial community in the bioaerosols was related to that of the aerosols' sources. The forced aeration of rotating brushes brought about observably distinct bacterial communities between sampling sites situated before and after the rotating brush. Isolation sources of closest relatives in bioaerosols clone libraries were associated with the aqueous environment in the WWTP. Common potential pathogens in bioaerosols as well as those not reported in previous research were also analyzed in this study. Measures should be adopted to reduce the emission of bioaerosols and prevent their exposure to workers. | Yunping Han Lin Li Junxin Liu | 2013 | Journal of Environmental Sciences2013，25，1： | 12 |
| 3 | Sulfur dioxide and o-xylene co-treatment in biofilter:Performance, bacterial populations and bioaerosols emissions显示文摘Sulfur dioxide(SO_2) and benzene homologs are frequently present in the off-gas during the process of sewage sludge drying. A laboratory scale biofilter was set up to co-treat SO_2 and o-xylene in the present study. SO_2 and o-xylene could be removed simultaneously in a single biofilter. Their concentration ratio in the inlet stream influenced the removal efficiencies. It is worth noting that the removal of SO_2 could be enhanced when low concentrations of o-xylene were introduced into the biofilter. Pseudomonas sp., Paenibacillus sp., and Bacillus sp. were the main functional bacteria groups in the biofilter. Sulfur-oxidizing bacteria(SOB) and o-xylene-degrading bacteria(XB) thrived in the biofilter and their counts as well as their growth rate increased with the increase in amount of SO_2 and o-xylene supplied. The microbial populations differed in counts and species due to the properties and components of the compounds being treated in the biofilter. The presence of mixed substrates enhanced the diversity of the microbial population. During the treatment process, bioaerosols including potentially pathogenic bacteria, e.g., Acinetobacter lwoffii and Aeromonas sp., were emitted from the biofilter. Further investigation is needed to focus on the potential hazards caused by the bioaerosols emitted from waste gas treatment bioreactors. | Yongli Sun Song Xue Lin Li Wenjie Ding Junxin Liu Yunping Han | 2018 | Journal of Environmental Sciences2018，30，7： | 7 |
| 4 | Effects of tetrakis (hydroxymethyl) phosphonium sulfate pretreatment on characteristics of sewage sludge显示文摘Recently, tetrakis(hydroxymethyl)phosphonium sulfate(THPS) was found to play an important role in the sludge pretreatment process. However, the effects of THPS pretreatment on the characteristics of sewage sludge are still insufficiently understood.The properties of sludge after pretreatment with different concentrations of THPS were investigated in this study. The results showed that pH, dewatering ability, and particle size of sludge decreased with increase in THPS concentration. The volatile suspended solids(VSS) and total suspended solids(TSS) of sludge also decreased slightly with increase in THPS concentration. The specific oxygen uptake rate(SOUR) results suggested that lower THPS concentrations(≤ 1.87 mg/g VSS) enhanced the activity of sludge, but higher concentrations(≥ 1.87 mg/g VSS) inhibited it. Gram-negative bacteria with peritrichous flagellation(such as Pseudomonas, Escherichia, and Faecalibacterium) were extremely sensitive to THPS. The decrease in specific most probable numbers(MPNs) of pathogens(total coliforms and Escherichia coli) with the increase in THPS concentration also proved the sterilization ability of THPS in the sludge pretreatment process. Pretreatment of sludge with concentrations of THPS higher than 37.41 mg/g VSS would meet the pathogen requirements for land application of Class A biosolids. | Yunping Han Wenzhe Zhang Xiao Yu Panfen Yu Benyi Xiao Hao Yi | 2019 | Journal of Environmental Sciences2019，31，4： | 5 |
| 5 | Enhancing nitrogen removal in an Orbal oxidation ditch by optimization of oxygen supply: practice in a full-scale municipal wastewater treatment plant显示文摘 | Xin Zhou Xuesong Guo Yunping Han Junxin Liu Jincheng Ren Yu Wang Yantao Guo | 2012 | Bioprocess and Biosystems Engineering2012，，7： | 1 |
| 6 | Study of a Novel Vertical Flow Constructed Wetland System with Drop Aeration for Rural Wastewater Treatment 显示文摘 | ZOU Juan GUO Xuesong HAN Yunping | 2012 | Water Air Soil Pollut2012，223，2： | 1 |
| 7 | Evaluation of bio- hydrogen production from glucose and protein at neutral initial pH 显示文摘 | Xiao Benyi Han Yunping Liu Junxin | 2010 | International Journal of Hydrogen Energy2010，35，12： | 1 |
| 8 | Evaluation of bio- hydrogen production from glucose and protein at neutral initial pH 显示文摘 | Xiao Benyi Han Yunping Liu Junxin | 2010 | International Journal of Hydrogen Energy2010，35，12： | 1 |
| 9 | Reversal of Multidrug Resistance of Adriamycin-resistant Gastric Adenocarcinoma Cells Through the Up-regulation of DARPP-32显示文摘 | Liu Hong Yunping Zhao Jin Wang Ying Han Wei Guo Haifeng Jin Huihong Zhai Feihu Bai Xiaoyin Zhang Taidong Qiao Zhen Chen Daiming Fan | 2008 | Digestive Diseases and Sciences2008，，1： | 1 |
| 10 | Micro-environment characteristics and microbial communities in activated sludge flocs of different particle size显示文摘 | Yunping Han Junxin Liu Xuesong Guo Lin Li | 2012 | Bioresource Technology2012，，： | 1 |
| 11 | Enhanced total nitrogen removal performance in a modified Orbal oxidation ditch system with internal nitrate recycle 显示文摘 | ZHOU Xin HAN Yunping GUO Xuesong | 2013 | Chemical Engineering Journal2013，228，： | 1 |
| 12 | Asse- ssing impact of irrigation water on groundwater recharge and quality in arid environment using CFCs, tritium and stable isotopes, in the Zhangye Basin, Northwest China 显示文摘 | Qin Dajun Qian Yunping Han Liangfeng | 2011 | Journal of Hydrology2011，405，12： | 1 |
| 13 | H2S removal and bacterial structure along a full-scale biofilter bed packed with polyurethane foam in a landfill site显示文摘 | Li Lin Han Yunping Yan Xu | 2013 | Bioresource Technology2013，147，17： | 1 |
| 14 | A broadly neutralizing antibody against SARS-CoV-2 Omicron variant infection exhibiting a novel trimer dimer conformation in spike protein binding显示文摘Dear Editor,The constant evolution of SARS-CoV-2 has resulted in the emergence of circulating variants during the COVID-19 pandemic.Recently,a newly emerged B.1.1.529(Omicron)variant raised global concern.Compared with the original SARS-CoV-2 strain and other variants of concern(VOCs),the Omicron variant has more than 30 mutations on its spike(S)protein,of which 16 mutations are located in the receptor-binding domain(RBD). | Yingdan Wang Wuqiang Zhan Jiangyan Liu Yanqun Wang Xiang Zhang Meng Zhang Lin Han Yunping Ma Lu Lu Yumei Wen Zhenguo Chen Jincun Zhao Fan Wu Lei Sun Jinghe Huang | 2022 | Cell Research2022，32，9： | 1 |
| 15 | Assessing impact of irrigation water on groundwater recharge and quality in arid environment using CFCs, tritium and stable isotopes, in the Zhangye Basin, Northwest China显示文摘 | Dajun Qin Yunping Qian Liangfeng Han Zhimin Wang Chen Li Zhanfeng Zhao | 2011 | Journal of Hydrology2011，，1： | 1 |
| 16 | A biofilter integrated with gas membrane separation unit for the treatment of fluctuating styrene loads显示文摘 | Li Lin Lian Jing Han Yunping | 2012 | Bioresource Technology2012，111，： | 1 |
| 17 | Study of a Novel Vertical Flow Constructed Wetland System with Drop Aeration for Rural Wastewater Treatment显示文摘 | Juan Zou Xuesong Guo Yunping Han Junxin Liu Hanwen Liang | 2012 | Water Air & Soil Pollution2012，，2： | 1 |
| 18 | Inorganic hollow mesoporous spheres-based delivery for antimicrobial agents显示文摘Microorganisms coexist with human beings and have formed a complex relationship with us.However,the abnormal spread of pathogens can cause infectious diseases thus demands antibacterial agents.Currently available antimicrobials,such as silver ions,antimicrobial peptides and antibiotics,have diverse concerns in chemical stability,biocompatibility,or triggering drug resistance.The“encapsulate-and-deliver”strategy can protect antimicrobials against decomposing,so to avoid large dose release induced resistance and achieve the controlled release.Considering loading capacity,engineering feasibility,and economic viability,inorganic hollow mesoporous spheres(iHMSs)represent one kind of promising and suitable candidates for real-life antimicrobial applications.Here we reviewed the recent research progress of iHMSs-based antimicrobial delivery.We summarized the synthesis of iHMSs and the drug loading method of various antimicrobials,and discussed the future applications.To prevent and mitigate the spread of an infective disease,multilateral coordination at the national level is required.Moreover,developing effective and practicable antimicrobials is the key to enhancing our capability to eliminate pathogenic microbes.We believe that our conclusion will be beneficial for researches on the antimicrobial delivery in both lab and mass production phases. | Yunping Qiao Yanyang Han Rengui Guan Shiliang Liu Xinling Bi Shanshan Liu Wei Cui Tao Zhang Tao He | 2023 | Frontiers of Materials Science2023，17，1： | 0 |
| 19 | Composition,dispersion,and health risks of bioaerosols in wastewater treatment plants:A review显示文摘Bioaerosols are defined as airbome particles(0.05-100 um in size)of biological origin.They are considered potentially harmful to human health as they can contain pathogens such as bacteria,fungi,and viruses.This review summarizes the most recent research on the health risks of bioaerosols emitted from wastewater treatment plants(WWTPs)in order to improve the control of such bioaerosols.The concentration and size distribution of WWTP bioaerosols;their major emission sources,composition,and health risks;and considerations for future research are discussed.The major themes and findings in the literature are as follows:the major emission sources of WWTP bioaerosols include screen rooms,sludge-dewatering rooms,and acration tanks;the bioaerosol concentrations in screen and sludge-dewatering rooms are higher than those outdoors.WWTP bioacrosols contain a variety of potentially pathogenic bacteria,fungi,antibiotic resistance genes,viruses,endotoxins,and toxic metal(loid)s.These potentially,pathogenic substances spread with the bioaerosols,thereby posing health risks to workers and residents in and around the WWTP.Inhalation has been identified as the main exposure route,and children are at a higher risk of this than adults.Future studies should identify emerging contaminants,establish health risk assessments,and develop prevention and control systems. | Yunping Han Lin Li Ying Wang Jiawei Ma Pengyu Li Chao Han Junxin Liu | 2021 | Frontiers of Environmental Science & Engineering2021，15，3： | 0 |
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