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| 1 | Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures显示文摘 | Bin-Kuan Chou Prashant Mali Xiaosong Huang Zhaohui Ye Sarah N Dowey Linda MS Resar Chunlin Zou Alex Zhang Jay Tong Linzhao Cheng | 2011 | Cell Research2011，21，3： | 38 |
| 2 | Development of a Chinese land data assimilation system:its progress and prospects显示文摘陆地数据吸收的目的是为改进陆地表面状态的评价把多来源观察合成陆地表面模型的动力学。我们为中国的陆地领土开发了一个陆地数据吸收系统。在这个系统,普通陆地模型和简单生物圈,模型 2 被用来模仿陆地表面过程。当转移的观察操作员为预言建模进估计的亮度温度,融化并且结冰的土壤,雪,湖,和植被的放射的转移模型被使用。一个 Monte-Carlo 顺序的过滤器,整体 Kalman 过滤器,作为数据吸收方法被实现集成当模特儿并且观察。系统能够遥远地吸收被动微波察觉到的数据象特殊传感器微波成像器那样(SSM 我) , TRMM 微波成像器(TMI ) ,和先进微波扫描辐射计为曙光女神(AMSRE ) 提高了并且在土壤和雪的 situmeasurements 常规。与 0.25 度的一个空间决定和一个小时的时间的分辨率,为土壤潮湿,土壤温度,雪和冻结的土壤的空间与时间地一致的吸收数据集被生产了。这篇论文介绍在数据吸收算法,陆地表面水文学变量的陆地表面建模微波遥感,和大气的强迫的数据的准备上做的中国陆地数据吸收系统和进步的开发。为未来开发开发陆地数据吸收系统和观点的不同特征和挑战也被讨论。 | Li Xin Huang Chunlin Che Tao Jin Rui Wang Shugong Wang Jiemin Gao Feng Zhang Shuwen Qiu Chongjian Wang Chenghai | 2007 | Progress in Natural Science:Materials International2007，17，8： | 30 |
| 3 | Modeling and Simulation on Axial Piston Pump Based on Virtual Prototype Technology显示文摘A particular emphasis is placed on the virtual prototype technology(VPT) of axial piston pump.With this technology it is convenient and flexible to build a complicated 3D virtual based on real physical model.The actual kinematics pairs of the parts were added on the model.The fluid characters were calculated by hydraulic software.The shape of the parts, the flexible body of parts, etc were improved in this prototype.So the virtual prototype of piston pump can work in computer like a real piston pump, and the flow ripple, pressure pulsation, motion principle, stress of parts, etc can be investigated.The development of the VPT is introduced at the beginning, and the modeling process of the virtual prototype is explained.Then a special emphasis is laid on the relationship between the dynamics model and the hydraulic model, and the simulations on the flow ripple, pressure pulsation, motion principle, the stress and strain distribution of the middle shaft and piston are operated.Finally, the advantages and disadvantages of the VPT are discussed.The improved virtual prototype of piston pump more tally with the real situation and the VPT has a great potential in simulation on hydraulic components. | ZHANG Bin XU Bing XIA Chunlin YANG Huayong | 2009 | Chinese Journal of Mechanical Engineering2009，22，1： | 29 |
| 4 | Neuroprotective effects of rutaecarpine on cerebral ischemia reperfusion injury显示文摘Rutaecarpine,an active component of the traditional Chinese medicine Tetradium ruticarpum,has been shown to improve myocardial ischemia reperfusion injury.Because both cardiovascular and cerebrovascular diseases are forms of ischemic vascular disease,they are closely related.We hypothesized that rutaecarpine also has neuroprotective effects on cerebral ischemia reperfusion injury.A cerebral ischemia reperfusion model was established after 84,252 and 504 μg/kg rutaecarpine were given to mice via intraperitoneal injection,daily for 7 days.Results of the step through test,2,3,5-triphenyl tetrazolium chloride dyeing and oxidative stress indicators showed that rutaecarpine could improve learning and memory ability,neurological symptoms and reduce infarction volume and cerebral water content in mice with cerebral ischemia reperfusion injury.Rutaecarpine could significantly decrease the malondialdehyde content and increase the activities of superoxide dismutase and glutathione peroxidase in mouse brain.Therefore,rutaecarpine could improve neurological function following injury induced by cerebral ischemia reperfusion,and the mechanism of this improvement may be associated with oxidative stress.These results verify that rutaecarpine has neuroprotective effects on cerebral ischemia reperfusion in mice. | Chunlin Yan Ji Zhang Shu Wang Guiping Xue Yong Hou | 2013 | Neural Regeneration Research2013，8，22： | 20 |
| 5 | Down.regulation of E.cadherin enhances prostate cancer chemoresistance via Notch signaling显示文摘Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was decreased.Interestingly,Notch-1 expression was up-regulated in PC3-Tx R and DU145-Tx R cells,whereas the E-cadherin expression was down-regulated in these cells comparing with their parental cells.The use ofγ-secretase inhibitor,a Notch signaling pathway inhibitor,significantly increased the sensitivity of chemoresistant cells to paclitaxel.Conclusion:The down-regulation of E-cadherin enhances PCa chemoresistance via Notch signaling,and inhibiting the Notch signaling pathway may reverse PCa chemoresistance. | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T. Keller Yi Lu Jian Zhang | 2017 | Chinese Journal of Cancer2017，36，3： | 16 |
| 6 | Simulation and study on mitigation measures of frequent subsynchronous oscillation with low amplitude at multi-power plants显示文摘Subsynchronous oscillation (SSO) with low amplitude that exceeds cumulative fatigue threshold of the generator shaft frequently could significantly reduce the shaft's service life, which is a new SSO problem that emerges in recent years. According to the real recording oscillograph, the basic reason for frequently over-threshold SSO with low amplitude at multi-power plants was analyzed based on Hulunbuir League system. The sensitivities of the electrical damping to the main electrical parameters in the contributing loop of subsynchronous torsional interaction were calculated. Based on the sensitivities, a simulation method was presented, which was used to excite the same oscillation as the actual case by exerting disturbance on the firing angle. The limitation of wide-band and narrow-band supplementary subsynchronous damping controller (SSDC) for mitigating this kind of SSO was analyzed based on the electromagnetic transient simulation model of Hulunbuir League system. The difference of supplementary excitation damping controller (SEDC) and parallel-form FACTS connected to the generator terminal was compared from the aspects of response time and the ability of damping torque supplying. The analysis indicates that their response time is similar but FACTS has stronger ability of damping torque supplying than SEDC. Time-domain simulation method was used to compare the mitigation effects of SEDC, static var compensator (SVC) and static synchronous compensator (STATCOM). Considering the mitigation effect, the floor space limit of the power plant and so on, STATCOM was considered as the best mitigation measure. A control strategy of cascaded STATCOM for engineering application was presented and the capacity for SSO mitigation as well as output characteristics was analyzed. The analysis indicates that STATCOM using the proposed control strategy has better mitigation effect and output characteristics with smaller capacity. | XIAO XiangNing ZHANG Jian GAO BenFeng WU YunSheng LUO Chao GUO ChunLin YANG Lin | 2013 | Science China(Technological Sciences)2013，56，6： | 13 |
| 7 | Simultaneous Screening of 733 Pesticide Residues in Fruits and Vegetables by a GC/LC-Q-TOFMS Combination Technique显示文摘In this paper,we report the construction of two accurate mass databases and the development of a combination detection method that simultaneously screens for 733 pesticide and chemical contaminant multi-residues via high-throughput liquid chromatography(LC)-and gas chromatography(GC)-quadru pole-time-of-flight mass spectrometry(Q-TOFMS).This work demonstrates that electronic mass spectral standards may replace chemical-source standard materials as references through one sample preparation and the combination of GC/LC-Q-TOFMS screening.This cutting-edge technique has also replaced multiresidue determination using targeted detection with non-targeted screening.The pesticide residue types,sensitivity,recovery,and reproducibility of this combination technique are evaluated in eight fruit and vegetable matrices.This technique shows three advantages:①In comparison with the discovery capability of a single technique,the combination technique shows an improvement of 51.1%(GC-QTOFMS)and 39.6%(LC-Q-TOFMS),respectively;②the combination technique can satisfy a screening limit lower than 10μg·kg^-1 and meet the requirements of“uniform standards,”although some of the pesticide residues could be optimized to further improve screening sensitivity;③over 488 pesticides with recoveries between 60%-120%and relative standard deviation(RSD)<20%at a spiked level of 10μg·kg^-1 were detected with the combination technique in eight different matrices.From 2012 to 2017,this combination technique was applied in an investigation to screen pesticide residues from 1384 sampling locations for 38138 batched samples covering 18 categories and 134 types of fruits and vegetables obtained from across the mainland of China.After statistical analysis,533 pesticides in 115891 determinations were detected,and the regularity of pesticides in the fruits and vegetables sold on the Chinese market was shown. | Guofang Pang Qiaoying Chang Ruobin Bai Chunlin Fan Zijuan Zhang Hongyuan Yan Xingqiang Wu | 2020 | Engineering2020，6，4： | 11 |
| 8 | Neural Networks Combined with Importance Sampling Techniques for Reliability Evaluation of Explosive Initiating Device显示文摘Concerning the issue of high-dimensions and low-failure probabilities including implicit and highly nonlinear limit state function, reliability analysis based on the directional importance sampling in combination with the radial basis function (RBF) neural network is used, and the RBF neural network based on first-order reliability method (FORM) is to approximate the unknown implicit limit state functions and calculate the most probable point (MPP) with iterative algorithm. For good efficiency, based on the ideas that directional sampling reduces dimensionality and importance sampling focuses on the domain contributing to failure probability, the joint probability density function of importance sampling is constructed, and the sampling center is moved to MPP to ensure that more random sample points draw belong to the failure domain and the simulation efficiency is improved. Then the numerical example of initiating explosive devices for rocket booster explosive bolts demonstrates the applicability, versatility and accuracy of the approach compared with other reliability simulation algorithm. | GONG Qi ZHANG Jianguo TAN Chunlin WANG Cancan | 2012 | Chinese Journal of Aeronautics2012，25，2： | 10 |
| 9 | Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging显示文摘Recently,transplantation of allogeneic and autologous cells has been used for regenerative medicine.A critical issue is monitoring migration and homing of transplanted cells,as well as engraftment efficiency and functional capability in vivo.Monitoring of superparamagnetic iron oxide(SPIO) particles by magnetic resonance imaging(MRI) has been used in animal models and clinical settings to track labeled cells.A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in vivo.Bone marrow mesenchymal stem cells(MSCs) have been extensively investigated for their various therapeutic properties,and exhibit the potential to differentiate into cells of diverse lineages.In this study,cynomolgus monkey MSCs(cMSCs) were labeled with Molday ION Rhodamine-BTM(MIRB),a new SPIO agent,to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in vivo.The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging.Our method is helpful for detection of transplanted stem cells in vivo,which is required for understanding mechanisms of cell therapy. | REN ZhenHua WANG JiaYin ZOU ChunLin GUAN YunQian ZHANG Yu Alex | 2011 | Science China(Life Sciences)2011，54，11： | 9 |
| 10 | 2,5-Furandicarboxylic acid production via catalytic oxidation of 5-hydroxymethylfurfural:Catalysts,processes and reaction mechanism显示文摘Biomass conversion to value-added chemicals has received tremendous attention for solving global warming issues and fossil fuel depletion.5-Hydroxymethylfurfural(HMF)is a key bio-based platform molecule to produce many useful organic chemicals by oxidation,hydrogenation,polymerization,and ring-opening reactions.Among all derivatives,the oxidation product 2,5-furandicarboxylic acid(FDCA)is a promising alternative to petroleum-based terephthalic acid for the synthesis of biodegradable plastics.This review analytically discusses the recent progress in the thermocatalytic,electrocatalytic,and photocatalytic oxidation of HMF into FDCA,including catalyst screening,synthesis processes,and reaction mechanism.Rapid fundamental advances may be possible in non-precious metal and metal-free catalysts that are highly efficient under the base-free conditions,and external field-assisted processes like electrochemical or photoelectrochemical cells. | Chunlin Chen Lingchen Wang Bin Zhu Zhenqiang Zhou Soliman I.El-Hout Jie Yang Jian Zhang | 2021 | Journal of Energy Chemistry2021，30，3： | 9 |
| 11 | Phylogenetic and genetic characterization of a 2017 clinical isolate of H7N9 virus in Guangzhou,China during the fifth epidemic wave显示文摘Pathogenic H7N9 influenza viruses continue to pose a public health concern. The H7N9 virus has caused five outbreak waves of human infections in China since 2013. In the present study, a novel H7N9 strain(A/Guangdong/8 H324/2017) was isolated from a female patient with severe respiratory illness during the fifth wave of the 2017 H7N9 epidemic. Phylogenetic analysis showed that the H7N9 viruses collected during the fifth wave belong to two different lineages: the Pearl River Delta lineage and the Yangtze River Delta lineage. The novel isolate is closely related to the Pearl River Delta H7N9 viruses, which were isolated from patients in Guangdong Province. The novel H7N9 isolate has an insertion of three basic amino acids in the cleavage site of hemagglutinin(HA), which may enhance virulence in poultry. The 2017 isolate also possesses an R292 K substitution in the neuraminidase(NA)protein, which confers oseltamivir resistance. This study highlights the pandemic potential of the novel H7N9 virus in mammals;thus, future characterization and surveillance is warranted. | Yongqiang Deng Chunlin Li Jianfeng Han Yingfen Wen Jian Wang Wenxing Hong Xiaofeng Li Zhongyu Liu Qing Ye Jing Li Changshuai Zhou Lei Yu Chengfeng Qin Fuchun Zhang Tao Jiang | 2017 | Science China(Life Sciences)2017，60，12： | 9 |
| 12 | Speciation analysis of metals (Tl,Cd and Pb) in Tl-containing pyrite and its cinder from Yunfu Mine,China,by ICP-MS with sequential extraction显示文摘The speciation of heavy metals such as thallium,cadmium and lead existing in pyrite and pyrite cinder was analyzed by ICP-MS with a sequential extraction procedure.The distribution patterns of these metals including exchangeable,reducible,oxidizable and residual fractions were obtained.Tl,Cd and Pb in pyrite and pyrite cinder samples from each extraction step were determined by inductively coupled plasma mass spectrometry(ICP-MS).Under the optimized instrumental conditions,detection limits of Tl,Cd and Pb in different matrices were within the range of 0.006-0.07 μg/L,and the relative standard deviations ranged from 0.8% to 1.2%.The accuracy of Tl,Cd and Pb determination was checked by analyzing two certified reference materials.The results demonstrate that trace Tl,Cd and Pb in the samples can be accurately determined.The sequential extraction results revealed that the percent contents of Tl,Cd and Pb in exchangeable,reducible and oxidizable fractions in pyrite cinder are different from those in pyrite and in pyrite cinder.Tl,Cd and Pb mostly are distributed in residual fraction.Therefore,the mobility of metals in pyrite is higher than that in pyrite cinder.Although distributions of Tl,Cd and Pb in the non-residual fraction are not dominant in pyrite cinder,the total concentrations of them could not be ignored.Consequently,attention must be paid to the risk of potential pollution by pyrite cinder. | WANG Chunlin CHEN Yongheng PAN Jianyang ZHANG Ping QI Jianying LIU Juan LI Xiangping WANG Jin | 2010 | Chinese Journal Of Geochemistry2010，29，1： | 9 |
| 13 | Pore throat characteristics of tight sandstone of Yanchang Formation in eastern Gansu,Ordos Basin显示文摘An important factor to evaluate reservoir quality is the pore-throat size.However,the strong heterogeneity makes it difficult to characterize the pore-throat distribution in tight reservoirs.The field emission scanning electron microscope(FESEM),high pressure mercury injection and rate-controlled mercury injection are used to investigate the pore-throat size distribution in tight sandstone reservoirs of Member 7 of the Yanchang Formation in eastern Gansu,Ordos Basin,and studies of the pore throat size controlling on physical property of the tight sandstone reservoirs are also carried out.The result shows that the pore type is mainly dominated by the residual intergranular pore,dissolution pore,micropore and a few micro-fractures;the high-pressure mercury injection experiment indicates that the pore-throat size ranges from 0.0148 μm to 40mm,the pore throat more than 1 mm is less;the ratecontrolled mercury injection experiment reveals that for samples with different physical properties,the pore radius mainly varies from 80 μm to 350 μm;the throat radius exhibits the strong heterogeneity,and is from 0.12 μm to 30μm;the pore-throat size can be effectively characterized by combination of high-pressure and rate-controlled mercury injections,and it varies from 0.0148 μm to 350 μm.The permeability is mainly controlled by the large pore throat(>R_(50))which accounts for a small proportion;in the tight sandstone with the permeability greater than 0.1 mD,the permeability is mainly controlled by the micropore and mesopore;in the tight sandstone with the permeability smaller than 0.1 mD,the permeability is mainly controlled by the nanopore and micropore;the proportion of small pore throat increases with reduction of permeability,it is important that the small pore throat influences the reservoir storage property though its effect on permeability are small. | Hao Wu Chunlin Zhang Youliang Ji Rui'e Liu Shang Cao Sheng Chen Yunzhao Zhang Ye Wang Wei Du Gang Liu | 2018 | Petroleum Research2018，3，1： | 7 |
| 14 | Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury显示文摘In the present study, rabbits were treated with hyperbaric oxygen for 1 hour after detonator-blast-induced craniocerebral injury. Immunohistochemistry showed significantly reduced aquaporin 4 expression and adrenocorticotropic hormone expression in the pituitary gland of rabbits with craniocerebral injury. Aquaporin 4 expression was positively correlated with adrenocorticotropic hormone expression. These findings indicate that early hyperbaric oxygen therapy may suppress adrenocorticotropic hormone secretion by inhibiting aquaporin 4 expression. | Jian Huo Jiachuan Liu Jinbiao Wang Yongming Zhang Chunlin Wang Yanyan Yang WenjiangSun Shaonian Xu | 2012 | Neural Regeneration Research2012，7，22： | 6 |
| 15 | Crack location identification of rotating rotor systems using operating deflection shape data显示文摘Crack location identification, as one key destination of structural health monitoring, is still a challenge for operating rotor systems. The operating deflection shape (ODS), which represents a visual description of the structural vibration patterns under operating conditions, has been gaining importance for structure damage detection in recent years. The ODS carries damage information of a structure, however, it is also difficult to detect weak cracks of rotor directly. The approximate waveform capacity dimension (AWCD) method was successfully applied to damage detection of plates and beam-like structures. In this paper,a strategic approach that combines ODS and weighted AWCD is proposed for crack location identification of the rotating rotor.To eliminate the false peaks of AWCD and obtain desirable results, a weight factor and ODS curvature data are introduced to the expression of the weighted AWCD. The effectiveness of the proposed method is validated by numerical simulation and experimental investigation in a cracked rotor system. The results indicate that the proposed approach not only provides good identifying performance for incipient rotor cracks, but also effectively eliminates the fault peaks introduced by the inflexion locations of ODSs. Moreover, the proposed approach proves promising in detecting crack locations of rotating rotor systems. | ZHANG ChunLin LI Bing YANG ZhiBo XIAO WenRong HE ZhengJia | 2013 | Science China(Technological Sciences)2013，56，7： | 6 |
| 16 | Inducible regulation of GDNF expression in human neural stem cells显示文摘Glial cell derived neurotrophic factor (GDNF) holds promises for treating neurodegenerative diseases such as Parkinson's disease. Human neural stem cells (hNSCs) have proved to be a suitable cell delivery vehicle for the safe and efficient introduction of GDNF into the brain. In this study, we used hNSCs-infected with a lentivirus encoding GDNF and the hygromycin resistance gene as such vehicles. A modified tetracycline operator 7 (tetO7) was inserted into a region upstream of the EF1- promoter to drive GDNF expression. After hygromycin selection, hNSCs were infected with a lentivirus encoding a KRAB-tetracycline repressor fusion protein (TTS). TTS bound to tetO7 and suppressed the expression of GDNF in hNSCs. Upon administration of doxycycline (Dox) the TTS-tetO7 complex separated and the expression of GDNF resumed. The hNSCs infected with GDNF expressed the neural stem cell specific markers, nestin and sox2, and exhibited no significant change in proliferation rate. However, the rate of apoptosis in hNSCs expressing GDNF was lower compared with normal NSCs in response to actinomycin treatment. Furthermore, a higher percentage of Tuj-1 positive cells were obtained from GDNF-producing NSCs under conditions that induced differentiation compared to control NSCs. The inducible expression of GDNF in hNSCs may provide a system for the controllable delivery of GDNF in patients with neurodegenerative diseases. | WANG ShuYan REN Ping GUAN YunQian ZOU ChunLin FU LinLin ZHANG Yu | 2013 | Science China(Life Sciences)2013，56，1： | 5 |
| 17 | Analysis of vegetation changes and dominant factors on the Qinghai-Tibet Plateau, China显示文摘This research was undertaken to clarify the characteristics of vegetation change and its main influencing factors on the Qinghai-Tibet Plateau. Using the greenness rate of change(GRC) and correlation factors, we analyzed the trend of vegetation change and its dominant factors from 2000 to 2015. The results indicate that the vegetation tended to improve from 2000 to 2015 on the Qinghai-Tibet Plateau, with the improved area accounting for 39.93% of the total; and the degraded area accounting for 19.32%. The areas of degraded vegetation are mainly concentrated in the low-relief and intermediate-relief mountains of the high-altitude and extremely high-altitude areas on the Qinghai-Tibet Plateau, as the vegetation characteristics are impacted by the terrain. Temperature and precipitation have obvious response mechanisms to vegetation growth, but the effects of precipitation and temperature on vegetation degradation are not significant over a short time frame. Overgrazing and population growth are the dominant factors of vegetation degradation on the Qinghai-Tibet Plateau. | HongWei Wang Yuan Qi ChunLin Huang XiaoYing Li XiaoHong Deng JinLong Zhang | 2019 | Research in Cold and Arid Regions2019，11，2： | 5 |
| 18 | Optimization of working parameters for puddling and flatting machine in paddy field显示文摘In order to improve the soil preparation quality,a wide puddling and flatting machine for paddy field was designed with the functionality of tillage,soil crushing,puddling,burying stubble and flatting in one-time operation,which can be equipped on tractors with high power.This study focused on the design and analysis of key components of the machine including puddling equipment,flat shovel and balance structure.Optimal results with field trials and response surface analysis showed that the machine can operate optimally when the working speed,puddling depth and angle of flat shovel were 1.05 km/h,16 cm and 31.30°,respectively.The surface flatness standard deviation was 3.7 cm,and slurry degree was 1.03 g/cm3.Field validation test results were consistent with the optimal parameters,which was able to meet agronomic requirements of mechanical planting in China. | Xu Chunlin Zhang Chengliang Li Lianhao Li Mingjin | 2016 | International Journal of Agricultural and Biological Engineering2016，9，3： | 5 |
| 19 | A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice,rabbits and NHPs显示文摘The emergence of SARS-CoV-2 variants and potentially other highly pathogenic sarbecoviruses in the future highlights the need for pan-sarbecovirus vaccines.Here,we discovered a new STING agonist,CF501,and found that CF501-adjuvanted RBD-FC vaccine(CF501/RBD-FC)elicited significantly stronger neutralizing antibody(nAb)and T cell responses than Alum-and cGAMP-adjuvanted RBD-FC in mice.Vaccination of rabbits and rhesus macaques(nonhuman primates,NHPs)with CF501/RBD-FC elicited exceptionally potent nAb responses against SARS-CoV-2 and its nine variants and 41 S-mutants,SARS-CoV and bat SARSr-CoVs.CF501/RBD-FC-immunized hACE2-transgenic mice were almost completely protected against SARS-CoV-2 challenge,even 6 months after the initial immunization.NHPs immunized with a single dose of CF501/RBD-FC produced high titers of nAbs.The immunized macaques also exhibited durable humoral and cellular immune responses and showed remarkably reduced viral load in the upper and lower airways upon SARS-CoV-2 challenge even at 108 days post the final immunization.Thus,CF501/RBD-Fc can be further developed as a novel pan-sarbecovirus vaccine to combat current and future outbreaks of sarbecovirus diseases. | Zezhong Liu Jie Zhou Wei Xu Wei Deng Yanqun Wang Meiyu Wang Qian Wang Ming Hsieh Jingming Dong Xinling Wang Weijin Huang Lixiao Xing Miaoling He Chunlin Tao Youhua Xie Yilong Zhang Youchun Wang Jincun Zhao Zhenghong Yuan Chuan Qin Shibo Jiang Lu Lu | 2022 | Cell Research2022，32，3： | 5 |
| 20 | A new species of the genus Crocidura from China based on molecular and morphological data(Eulipotyphla: Soricidae)显示文摘A new species of white-toothed shrew,Crocidura anhuiensis Zhang,Zhang&Li,sp.nov.is described from Mount Huang,China.Genetic sequence(mt DNA Cyt b gene)and morphological(external and skull)data are used to distinguish this newly discovered species.The phylogenetic analysis shows that the materials of this work are monophyletic and allied to C.attenuata by the uncorrected genetic distance 4.9–5.1%,which suggests a species-level divergence.Morphologically,the materials are different from C.attenuata by presenting a greater proportion of tail-to-body ratio and the presence of an obvious tooth root in the mandible.Based on those data,the unnamed species is described as a new species which is currently known only from the Wild Monkey Valley,Mount Huang,Anhui Province,China. | Heng Zhang Guiyou Wu Yanqing Wu Jianfei Yao Shuo You Chencheng Wang Feng Cheng Jingjing Chen Mingxia Tang Chunlin Li Baowei Zhang | 2019 | Zoological Systematics2019，44，4： | 5 |
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