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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | Phylogenetic and genetic characterization of a 2017 clinical isolate of H7N9 virus in Guangzhou,China during the fifth epidemic wave显示文摘Pathogenic H7N9 influenza viruses continue to pose a public health concern. The H7N9 virus has caused five outbreak waves of human infections in China since 2013. In the present study, a novel H7N9 strain(A/Guangdong/8 H324/2017) was isolated from a female patient with severe respiratory illness during the fifth wave of the 2017 H7N9 epidemic. Phylogenetic analysis showed that the H7N9 viruses collected during the fifth wave belong to two different lineages: the Pearl River Delta lineage and the Yangtze River Delta lineage. The novel isolate is closely related to the Pearl River Delta H7N9 viruses, which were isolated from patients in Guangdong Province. The novel H7N9 isolate has an insertion of three basic amino acids in the cleavage site of hemagglutinin(HA), which may enhance virulence in poultry. The 2017 isolate also possesses an R292 K substitution in the neuraminidase(NA)protein, which confers oseltamivir resistance. This study highlights the pandemic potential of the novel H7N9 virus in mammals;thus, future characterization and surveillance is warranted. | Yongqiang Deng Chunlin Li Jianfeng Han Yingfen Wen Jian Wang Wenxing Hong Xiaofeng Li Zhongyu Liu Qing Ye Jing Li Changshuai Zhou Lei Yu Chengfeng Qin Fuchun Zhang Tao Jiang | 2017 | Science China(Life Sciences)2017，60，12： | 9 |
| 3 | 寨卡病毒引起小鼠睾丸损伤并导致雄性不育显示文摘虽然寨卡病毒与其他黄病毒属成员,如登革热病毒、乙型脑炎病毒和两尼罗病毒都是通过蚊媒传播,但是前人的研究发现寨卡病毒在精液中存在数月之久,暗示其性接触传播的可能性。本研究表明寨卡病毒影响雄性生殖系统,提示了其对男性健康的潜在危害。 | Wenqiang Ma Shihua Li Shuoqian Ma Lina Jia Fuchun Zhang Yong Zhang Jingyuan Zhang Gary Wong Shanshan Zhang Xuancheng Lu Mei Liu Jinghua Yan Wei Li Chuan Qin Daishu Han Chengfeng Qin Na Wang 李向东 高福 | 2017 | 科学新闻2017，19，4： | 5 |
| 4 | Classification of CBERS-2 Imagery with Fuzzy ARTMAP Classifier显示文摘A fuzzy ARTMAP classifier is adopted for a classification experiment of CBERS-2 imagery. The fundamental theory and processing about the algorithm are first introduced, followed with a land-use classification experiment in Shihezi County on CBERS-2 high resolution imagery. Three classifiers are compared: maximum likelihood classifier (MLC), error back propagation (BP) classifier, and fuzzy ARTMAP classifier. The comparison shows comparably better results for the fuzzy ARTMAP classifier, with overall classification accuracy of 9.9% and 4.6% higher than that of MLC and BP. The results also prove that the fuzzy ARTMAP classifier has better discernment in identifying bare soil on CBERS-2 imagery. | LUO Chengfeng LIU Zhengjun YAN Qin | 2007 | Geo-Spatial Information Science2007，10，2： | 3 |
| 5 | Real-timecontrol of 3D virtual human motion using adepth-sensing camera for agricultural machinery training显示文摘 | Wang Chengfeng Ma Qin Zhu Dehai | 2013 | Mathematical and Computer Modelling (S0895-7177)2013，58，： | 1 |
| 6 | Structures and Receptor Binding of Hemagglutinins from Human-Infecting H7N9 Influenza Viruses显示文摘 | Yi Shi Wei Zhang Fei Wang Jianxun Qi Ying Wu Hao Song Feng Gao Yuhai Bi Yanfang Zhang Zheng Fan Chengfeng Qin Honglei Sun Jinhua Liu Joel Haywood Wenjun Liu Weimin Gong Dayan Wang Yuelong Shu Yu Wang Jinghua Yan George F. Gao | 2013 | Science2013，，6155： | 1 |
| 7 | Guidelines for the Diagnosis and Treatment of Dengue in China显示文摘Dengue is the most prevalent and rapidly spreading mosquito-borne viral disease.As a dengue non-endemic country,China has experienced several dengue outbreaks in recent years.However,dengue patients in China display clinical characteristics that are distinct from those of patients in dengue-endemic countries.To standardize the diagnosis and treatment of dengue fever,the experts at the Society of Infectious Diseases,Society of Tropical Medicine and Parasitology of the Chinese Medical Association,and the Society of Emergency Medicine of China Association of Chinese Medicine have developed a guideline after reviewing the following guidelines and data:guidelines for diagnosis,treatment,prevention,and control of dengue(World Health Organization,2009);the guidelines for diagnosis and treatment of dengue(National Health and Family Planning Commission of the People’s Republic of China,2014,Edition 2);health industry standard of the People’s Republic of China“diagnosis for dengue fever(WS216-2018)”;and systemic reports on dengue.The new guideline includes eight aspects:introduction,terminology,epidemiology and prevention,etiology and pathogenesis,clinical features,diagnosis,treatment,and problems to be solved. | Fuchun Zhang Jianfeng He Jie Peng Xiaoping Tang Chengfeng Qin Hongzhou Lu Xingwang Li Qingquan Liu Hong Zhao Jifang Sheng Guiqiang Wang Society of Infectious Diseases,Society of Tropical Medicine and Parasitology,Chinese Medical Association Society of Emergency Medicine,China Association of Chinese Medicine | 2021 | Infectious Diseases & Immunity2021，1，3： | 1 |
| 8 | Proteome-wide epitope mapping identifies a resource of antibodies for SARS-CoV-2 detection and neutralization显示文摘Dear Editor,Since December 2019,the coronavirus disease 2019(COVID-19)caused by the severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has become a worldwide pandemic.1 Significant efforts have been made to generate antibodies to help study COVID-19 pathogenesis,perform diagnostic testing,and develop treatment to neutralize SARS-CoV-2 activity.However,the specific sequence of amino acids recognized and bound by an antibody,the'epitope'is unknown for most antibodies.In this study,we developed a high-throughput epitope mapping platform using a peptide-based SARS-CoV-2 proteome microarray.2 to detect the binding epitopes of 57 commercial antibodies to ORFlab,nudeocapsid(N);spike(S),envelop(E),membrane(M),ORF3a,ORF6,ORF7a,and ORF8 proteins(Supplementary Table S1). | Te Liang Mengli Cheng Fei Teng Hongye Wang Yongqiang Deng Jiahui Zhang Chengfeng Qin Shubin Guo Hui Zhao Xiaobo Yu | 2021 | Signal Transduction and Targeted Therapy2021，6，5： | 0 |
| 9 | Expression pattern and function of SARS-CoV-2 receptor ACE2显示文摘Since the outbreak at the end of 2019,SARS-CoV-2 has been spreading around the world for more than one year.Scientists have been intensely conducting research on this newly emerged coronavirus and the disease caused by it.Angiotensin-converting enzyme 2(ACE2),as a receptor mediating the cellular entry of SARSCoV-2,has become a hot spot for researchers.Here,we summarized the recent progresses on the function,expression and distribution characteristics of ACE2 in human body and among populations.We further discussed the interaction mechanism of ACE2 and SARS-CoV-2 S protein,focusing on key residues that effect interaction and binding ability of SARS-CoV-2 variants.This will facilitate researchers to better understand SARS-CoV-2 infection and transmission route,adaptation mechanism,and designing treatment strategies. | Ruiting Li Chengfeng Qin | 2021 | Biosafety and Health2021，3，6： | 0 |
| 10 | A redox-responsive self-assembling COA-4-arm PEG prodrug nanosystem for dual drug delivery suppresses cancer metastasis and drug resistance by downregulating hsp90 expression显示文摘Metastasis and resistance are main causes to affect the outcome of the current anticancer therapies.Heat shock protein 90(Hsp90)as an ATP-dependent molecular chaperone takes important role in the tumor metastasis and resistance.Targeting Hsp90 and downregulating its expression show promising in inhibiting tumor metastasis and resistance.In this study,a redox-responsive dual-drug nanocarrier was constructed for the effective delivery of a commonly used chemotherapeutic drug PTX,and a COAmodified 4-arm PEG polymer(4PSC)was synthesized.COA,an active component in oleanolic acid that exerts strong antitumor activity by downregulating Hsp90 expression,was used as a structural and functional element to endow 4PSC with redox responsiveness and Hsp90 inhibitory activity.Our results showed that 4PSC/PTX nanomicelles efficiently delivered PTX and COA to tumor locations without inducing systemic toxicity.By blocking the Hsp90 signaling pathway,4PSC significantly enhanced the antitumor effect of PTX,inhibiting tumor proliferation and invasiveness as well as chemotherapy-induced resistance in vitro.Remarkable results were further confirmed in vivo with two preclinical tumor models.These findings demonstrate that the COA-modified 4PSC drug delivery nanosystem provides a potential platform for enhancing the efficacy of chemotherapies. | Yi Zhou Yingling Miao Qiudi Huang Wenwen Shi Jiacui Xie Jiachang Lin Pei Huang Chengfeng Yue Yuan Qin Xiyong Yu He Wang Linghao Qin Jianhai Chen | 2023 | Acta Pharmaceutica Sinica B2023，13，7： | 0 |
| 11 | Autographa Californica Multiple Nucleopolyhedrovirus orf13 Is Required for Efficient Nuclear Egress of Nucleocapsids显示文摘Autographa californica multiple nucleopolyhedrovirus(Ac MNPV)orf13(ac13)is a conserved gene in all sequenced alphabaculoviruses.However,its function in the viral life cycle remains unknown.In this study,we found that ac13 was a late gene and that the encoded protein,bearing a putative nuclear localization signal motif,colocalized with the nuclear lamina.Deletion of ac13 did not affect viral genome replication,nucleocapsid assembly or occlusion body(OB)formation,but reduced virion budding from infected cells by approximately 400-fold compared with the wild-type virus.Deletion of ac13 substantially impaired the egress of nucleocapsids from the nucleus to the cytoplasm,while the OB morphogenesis was unaffected.Taken together,our results indicated that ac13 was required for efficient nuclear egress of nucleocapsids during virion budding,but was dispensable for OB formation. | Xingang Chen Xiaoqin Yang Chengfeng Lei Fujun Qin Xiulian Sun Jia Hu | 2021 | Virologica Sinica2021，36，5： | 0 |
| 12 | Reverse spillover of SARS-CoV-2 from human to wild animals显示文摘The heavily transmitted and mutated SARS-CoV-2 has constitutes a sustained threat to global health.A recent article published in Nature,firstly provided solid evidence confirming the cross-species transmission of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)from human to free-ranging white-tailed deer(Hale et al.,2022).More importantly,circulation of SARS-CoV-2 among deer rapidly resulted into new phylogenetic clades of deer-only viruses which were believed linking to the high infection rate of SARS-CoV-2 within white-tailed deer in northeast Ohio,USA(Hale et al.,2022). | Mingyuan Li Xiaohe Cheng Chengfeng Qin | 2022 | Science China(Life Sciences)2022，65，9： | 0 |
| 13 | Oral administration of D-glucosamine confers broad-spectrum protection against human coronaviruses including SARS-CoV-2显示文摘Dear Editor,The novel human coronavirus(HCoV)SARS-CoV-2 is the causative agent of the current pandemic COVID-19,posing a huge threat to global public health.1 Up to now,seven HCoVs have been identified.These HCoVs(including SARS-CoV,HCoV-229E,HCoV-NL63,HCoV-OC43,HCoV-HKU1,MERS-CoV,and SARS-CoV-2)cause a range of symptoms from the common cold to severe pathologies. | Qi Qi Qi Chen Yumei Dong Kun Wang Jialu Wang Guiming Jin Aiping Zheng Rong Zhang Yongqiang Deng Yuhuan Li Chengfeng Qin Xiaotao Duan | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 0 |
| 14 | Development of a rapid neutralizing antibody test for SARS-CoV-2 and its application for neutralizing antibody screening and vaccinated serum testing显示文摘Background:Since the outbreak of coronavirus disease(COVID-19),the high infection rate and mutation fre-quency of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent,have contributed to the ongoing global pandemic.Vaccination has become the most effective means of controlling COVID-19.Tra-ditional neutralizing tests of sera are complex and labor-intensive,therefore,a rapid test for detecting neutralizing antibodies and antibody status post-immunization is needed.Methods:Based on the fact that antibodies exhibit neutralizing activity by blocking the binding of the S protein receptor-binding domain(S-RBD)to ACE2,we developed a rapid neutralizing antibody test,ACE2-Block-ELISA.To evaluate the sensitivity and specificity,we used 54 positive and 84 negative serum samples.We also tested the neutralizing activities of monoclonal antibodies(mAbs)and 214 sera samples from healthy individuals im-munized with the inactivated SARS-CoV-2 vaccine.Results:The sensitivity and specificity of the ACE2-Block ELISA were 96.3%and 100%,respectively.For neu-tralizing mAb screening,ch-2C5 was selected for its ability to block the ACE2-S-RBD interaction.A plaque assay confirmed that ch-2C5 neutralized SARS-CoV-2,with NT 50 values of 4.19,10.63,and 1.074μg/mL against the SARS-CoV-2 original strain,and the Beta and Delta variants,respectively.For the immunized sera samples,the neutralizing positive rate dropped from 82.14%to 32.16%within 4 months post-vaccination.Conclusions:This study developed and validated an ACE2-Block-ELISA to test the neutralizing activities of an-tibodies.As a rapid,inexpensive and easy-to-perform method,this ACE2-Block-ELISA has potential applications in rapid neutralizing mAb screening and SARS-CoV-2 vaccine evaluation. | Yuchang Li Mingyue Wang Hongzhen Wu Hui Zhao Lei Dong Yunfei Li Xiaofeng Li Ying Tang Sen Zhang Jing Li Chengfeng Qin Tao Jiang Yongqiang Deng Xiaoping Kang | 2022 | Infectious Medicine2022，1，2： | 0 |
| 15 | RNAi can be an antiviral immunity in mammals显示文摘Subject Code:H19With the support by the National Natural Science Foundation of China,a collaborative study by the research groups led by Prof.Zhou Xi(周溪)from the State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences and Prof.Qin Chengfeng(秦成峰)from Beijing Institute | Zhou Xi (周溪) Qin Chengfeng (秦成峰) | 2017 | Science Foundation in China2017，25，3： | 0 |
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