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| 1 | The culture and establishment of embryonic germ(EG)cell lines from Chinese mini swine显示文摘As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation ofembryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishmentof two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 anda 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, markercharacterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentiallyuseful for further basic studies. | HSlAOCHIENTSUNG ZHONGWEIDU RONGRUI XIULANLI LINPINGBAO JUNWU SHIMINBAO ZHENYAO | 2003 | Cell Research2003，13，3： | 17 |
| 2 | Isolation and characterization of the murine Nanog gene promoter显示文摘Nanog protein is expressed in the interior cells of compacted morulae and maintained till epiblasts but downregulated by implantation stage. It is also expressed in embryonic stem cells, embryonic carcinoma cells and embryonic germ cells but disappeared in differentiated ES cells. In this study, we have isolated, sequenced, and performed the first characterization of the Nanog promoter. The transcription start sites were mapped by primer extension analysis. Two promoter regions were found upstream the transcription start sites and the expression of major Nanog promoter/ reporter gene construct is abolished in differentiated F9 EC cells as compared to the undifferentiated counterpart. We also showed that a putative octamer motif (ATGCAAAA) is necessary for the major promoter activity. Gel shift and supershift assays showed that Oct-1, Oct-4 and Oct-6 protein selectively bind to the octamer motif. | DaYongWU ZhenYAO | 2005 | Cell Research2005，15，5： | 13 |
| 3 | The role of sediment resuspension duration in releaseof PAHs显示文摘Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants. Due to their low water solubility and high hydrophobicity, PAHs are rapidly sorbed onto particles and subsequently deposit in sediments once introduced into aquatic environment. In such a way, sediments become a huge sink for PAHs. During sediment resuspension, the potential exists for PAHs to be released from sediments into water. Sediment resuspension plays an important role in the transportation and fate of PAHs in the aquatic environment. In this study, release behavior of PAHs on Yangtze River sediment during resuspension was investigated using a particle entrainment simulator (PES). The role of re- suspension duration on release of 16 PAHs was measured by resuspending sediment for 12 h at 0.2 and 0.5 N/m2, respectively. Results indicated that PAH concentrations in TSS increase over time with more increase of phenanthrene and 4-ring PAHs. Comparing with 0.2 N/m2 (30%), ∑PAHs concentra- tions in TSS demonstrated remarkable increase during 0.5 N/m2 resuspension (37%). Dissolved PAH concentrations increased throughout the duration with more increase of 2―3 ring PAHs (50%―88%). Dissolved PAH concentrations showed remarkable increase during 0.5 N/m2 resuspension (50%). Moreover, PAH concentrations in overlying water throughout the duration of resuspension were higher than toxic effects threshold values in drinking water developed by WHO, which may cause toxic effect on ecosystem. | FENG JingLan SHEN ZhenYao NIU JunFeng YANG ZhiFeng | 2008 | Chinese Science Bulletin2008，53，18： | 9 |
| 4 | Is urban development an urban river killer? A case study of Yongding Diversion Channel in Beijing, China显示文摘The high population and concrete environment alter urban areas by changing temperature, rainfall runoff, and water resource utilization activities. This study was conducted to investigate the water quality features of the Yongding Diversion Channel in Beijing, China, and its relationship with rainfall and urban development. Monthly water quality data were obtained from April to October of 2004 at monitoring sites of Sanjiadian, Gaojing, Luodaozhuang, and Yuyuangtan. The monthly water quality grades from 2007 to 2011 were also investigated and compared with those of other rivers. Dissolved oxygen and pH showed greater decreases after one or two moderate rainfall events than several light rainfall events. The potassium permanganate index(CODMn), ammonia nitrogen(NH3-N) and total phosphorus(TP) increased more after several light rainfall events than after one or two moderate or heavy rainfall events. Pollutant concentrations(CODMn, NH3-N, TP) in downstream regions showed greater changes than those in upstream areas after heavy rainfall events. Intense human activities around the channel greatly influenced the water quality of the channel in rainy season because of runoff pollution; however, heavy rainfall had a strong dilution effect on the pollutant concentrations in rivers. Overall, urban development has obviously deteriorated the water quality of the Yongding Diversion Channel as indicated by an increase in the water quality index from 3.22 in 2008 to 4.55 in2010. The Pearson correlation between monthly rainfall and water quality indices from 2007 to 2011ranged from 0.1286 to 0.6968, generally becoming weaker as rainfall and rainfall runoff became more random and extreme. | Xi Wang Junqi Li Yingxia Li Zhenyao Shen Xuan Wang Zhifeng Yang Inchio Lou | 2014 | Journal of Environmental Sciences2014，26，6： | 8 |
| 5 | The electron structure and photocatalytic activity of Ti(IV) doped Bi_2O_3显示文摘Density functional theory (DFT) plays a significant role in the development of visible light responsive photocatalysts.Based on the first-principles plane-wave ultrasoft pseudopotential (USPP) method,the crystal structures of α,β,γ,and δ-Bi2O3 were optimally calculated for the total density of states (TDOS) and the partial density of states (PDOS) of Bi,O atoms.The calculation for Ti(IV) doped Bi2O3 supercell was carried out.The effects of Ti(IV)-doping on the electron structures and light absorption properties of various Bi2O3 were analyzed.The results showed that Ti 3d orbital appeared in the forbidden band of Bi2O3 and hybridized with O 2p,Bi 6p orbitals.The narrowed band gap (Eg) and red-shift of light absorption edge are responsible for the enhanced photocatalytic activity of Bi2O3.The β-Bi2O3 and Ti-doped β-Bi2O3 were prepared by a hydrothermal synthesis method.The improvement of the photocatalytic activity of Bi2O3 has also been verified by the characteristics of the UV-vis diffuse reflection spectrum and the experimental evaluation of the photocatalytic degradation of crystal violet in aqueous solution. | YIN LiFeng NIU JunFeng SHEN ZhenYao SUN Ying | 2011 | Science China Chemistry2011，54，1： | 7 |
| 6 | Atmospheric Corrosion of Copper Exposed in a Simulated Coastal-Industrial Atmosphere显示文摘The corrosion behavior of copper exposed in a simulated coastal-industrial atmosphere has been investigated using weight loss measurement, scanning electron microscopy, X-ray diffraction, potentiodynamic polarization and in-situ electrochemical impedance spectroscopy(EIS) with micro-distance electrodes.The results show that corrosion kinetics follows the empirical equation D = Atn. The main corrosion products are composed of Cu_2O, Cu_2Cl(OH)_3 and Cu_4Cl_2(OH)_6. A two-layer structure comprising a loose outer layer and a compact inner layer forms the corrosion products during corrosion process. SO_2 has been found to promote the formation of Cu_4Cl_2(OH)_6. | Chen Pan Wangyan Lv Zhenyao Wang Wei Su Chuan Wang Shinian Liu | 2017 | Journal of Materials Science & Technology2017，33，6： | 6 |
| 7 | An overview of research on agricultural non-point source pollution modelling in China显示文摘 | Zhenyao Shen Qian Liao Qian Hong Yongwei Gong | 2011 | Separation and Purification Technology2011，，： | 5 |
| 8 | Corrosion behavior of copper T2 and brass H62 in simulated Nansha marine atmosphere显示文摘The accelerated corrosion behavior of copper T2 and brass H62 exposed in simulated Nansha marine atmosphere for different periods were investigated by weight loss method, SEM, XRD and potentiodynamic polarization measurements. The results indicate that copper T2 and brass H62 underwent severe corrosion, and the final corrosion rates at 32 days of exposure were 0.24 (μm/d and 0.10 (xm/d, respectively. Moreover, the overall corrosion type of copper T2 was uniform and the corrosion products Cu2O and Cu2C1(OH)3 played a vital role in the corrosion rate of copper. While the dezincification corrosion with zinc preferential dissolution was obvious in brass H62. The predominant phases were the zinc-rich compounds Zn5(OH)8Cl2 H2O, Zni2(SO4)3Cl3(OH)15 5H20 and NaZn4(SO4)Cl(OH)6.6H2O. There existed a large number of copper-rich holes with 20-50μm depth beneath the corrosion product layer. | Xiao Lu Yuwei Liu Miaoran Liu Zhenyao Wang | 2019 | Journal of Materials Science & Technology2019，35，9： | 5 |
| 9 | Effect of sulfate-reducing bacteria on corrosion of X80 pipeline steel under disbonded coating in a red soil solution显示文摘Effect of sulfate-reducing bacteria on the corrosion behaviors of API-X80 pipeline steel has been studied in crevices under simulated disbonded coatings in red soil solution.The results show that there are amounts of SRB in the crevice under the simulated disbonded coating in the red soil solution during the whole period of experiment.The electrochemical impedance spectroscopy shows that SRB can enhance the corrosion of the steel in the crevice under the simulated disbonded coating,and the thermodynamic analyses indicate that SRB can accelerate the corrosion of steel using not only sulfate but also ferric hydroxide as electron acceptors. | Boxin Wei Jin Xu Qi Fua Qingyu Qin Yunlong Bai Cheng Sun Chuan Wang Zhenyao Wang Wei Ke | 2021 | Journal of Materials Science & Technology2021，，28： | 4 |
| 10 | Assessment of effects of best management practices on agricultural non-point source pollution in Xiangxi River watershed显示文摘 | Ruimin Liu Peipei Zhang Xiujuan Wang Yaxin Chen Zhenyao Shen | 2013 | Agricultural Water Management2013，，： | 3 |
| 11 | Development and test of the Export Coefficient Model in the Upper Reach of the Yangtze River显示文摘 | Xiaowen Ding Zhenyao Shen Qian Hong Zhifeng Yang Xing Wu Ruimin Liu | 2009 | Journal of Hydrology2009，，3： | 3 |
| 12 | Corrosion Behavior of 316L Stainless Steels Exposed to Salt Lake Atmosphere of Western China for 8 years显示文摘The evolution of corrosion behavior of 316 L stainless steels exposed to salt lake atmosphere for 8 years was investigated.The results showed that the stainless steel in salt lake atmosphere had a greater corrosion rate during the initial exposure time and relatively lower corrosion rate during the subsequent exposure time.Dust depositions accumulated on the downward surface caused severe local corrosion of stainless steel.As exposure time prolonged,the relative amount of Croxide and Feoxidein the corrosion products gradually increased,which may directly affect the corrosion rate of stainless steels.Moreover,the maximum pit depth followed a power function with respect to exposure time. | Mingxiao Guo Junrong Tang Tianzhen Gu Can Peng Qiaoxia Li Chen Pan Zhenyao Wang | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，4： | 3 |
| 13 | Effect of Holding Time on the Microstructure and Mechanical Properties of Dual-Phase Steel during Intercritical Annealing显示文摘Continuous annealing simulation tests were conducted by using a continuous annealing thermomechanical simulator. Holding times of 5, 60, 180, and 480 seconds for an intercritical annealing temperature of 820 ℃ were adopted to investigate the evolution of the microstructure and mechanical properties of ferrite-bainite dual-phase steel. The ferrite-bainite dual-phase steel was characterized by high strength and low yield ratio due to the presence of the constituents(polygonal ferrite, bainite, martensite and retained austenite) of the steel microstructure. Specimen 3 exhibits the highest value of A50(7.67%) and a product of Rm × A50(10453MPa%) after a 180 s holding. This is likely attributed to the presence of a C-enriched retained austenite in the microstructure. And the effect of martensite islands and carbide precipitate is thought to be able to contribute in strengthening the present steel. It is expected that equilibrium of austenite fraction would be reached for reasonable intercritical holding period, regardless of the heating temperature. The results suggest that long increasing holding times may not be needed because the major phase of the microstructure does not change very significantly. It is favorable for industrial production of DP steels to shorten holding times. | 李壮 WU Di Lü Wei YU Huanhuan SHAO Zhenyao LUO Lei | 2015 | Journal of Wuhan University of Technology(Materials Science)2015，30，1： | 3 |
| 14 | Distribution and sources of polycyclic aromatic hydrocarbons in Wuhan section of the Yangtze River, China显示文摘 | Chenglian Feng Xinghui Xia Zhenyao Shen Zhui Zhou | 2007 | Environmental Monitoring and Assessment (-)2007，，1： | 2 |
| 15 | Integrated assessment and changes of ecological environment in the Daning River Watershed显示文摘Based on Remote Sensing(RS),Geographical Information Systems(GIS),and the Spatial Principal Compo-nent Analysis(SPCA)method,the integrated assessment and changes in the ecological environment of Daning River Watershed are studied in this paper.The watershed is located in the Three Gorge Area in China.The result of the integrated assessment showed that level 9 had the biggest proportion in the year 1990,which was about 40%.In the year 2000,however,there were no levels with a proportion significantly bigger than the others.By comparing the assessment results in 1990 and 2000,it is discovered that the ecological environ-ment in Daning River Watershed in 1990 was better than that in 2000. | LIU Ruimin SHEN Zhenyao | 2007 | Frontiers in Biology2007，2，4： | 2 |
| 16 | Release of polycyclic aromatic hydrocarbons from Yangtze River sediment cores during periods of simulated resuspension显示文摘 | Zhifeng Yang Jinglan Feng Junfeng Niu Zhenyao Shen | 2007 | Environmental Pollution2007，，2： | 2 |
| 17 | Experimental and numerical investigation of threedimensional vortex structures of a pitching airfoil at a transitional Reynolds number显示文摘This research examines the vortex behaviors and aerodynamic forces in dynamic stall phenomena at a transitional Reynolds number(Re=90000)using experimental and numerical approaches.Periodic sinusoidal pitching motion at two different reduced frequencies is used to achieve the dynamic stall of a NACA 0012 airfoil.Several leading edge vortices form and detach in the dynamic stall stage.The flow then quickly transitions to a full separation zone in the stall stage when the angle of attack starts to decrease.There is discrepancy between the phaseaveraged and instantaneous flow field in that the small flow structures increased with angle of attack,which is a characteristic of the flow field at the transitional Reynolds number.The interaction between the streamwise vortices in the three-dimensional numerical results and the leading edge vortex are the main contribution to the turbulent flow.In addition,the leading edge vortex that supplies vortex lift is more stable at higher reduced frequency,which decreases the lift fluctuation in the dynamic stall stage.The leading edge vortex at higher reduced frequency is strong enough to stabilize the flow,even when the airfoil is in the down-stroke phase. | Zhenyao LI Lihao FENG Hamid Reza KARBASIAN Jinjun WANG Kyung Chun KIM | 2019 | Chinese Journal of Aeronautics2019，32，10： | 2 |
| 18 | Nanosecond pulsed laser-assisted modified copper surface structure:Enhanced surface microhardness and microbial corrosion resistance显示文摘Microbiologically influenced corrosion(MIC) is an unavoidable problem in several industries. Copper(Cu)and its alloys are widely used engineering materials. However, MIC of Cu remains a persistent challenge to their performance and functional lifetime under aggressive environments. This study investigated nanosecond pulsed laser processing(LP), which may enhance the corrosion resistance of Cu. The microstructural evolution and corrosion behavior of LP-Cu in the presence of sulfate-reducing bacteria(SRB) were evaluated. Typical deformation-induced microstructural features of high-density dislocations were analyzed on the top surface of LP-Cu coupon. Electrochemical measurements suggested that LP-Cu coupons exhibited better corrosion resistance in SRB-inoculated solution compared with their original counterpart. The enhanced corrosion resistance by LP primarily resulted from the combined influences of compressive residual stress and work hardening in the surface. However, overlap percentage played a key role in improving corrosion resistance. LP produced optimal corrosion resistance at 50% overlap. Therefore, this study introduces a unique and an option for anticorrosion control in manufacturing processes and potentially implements it onto other materials to improve its microbial corrosion resistance through LP. | Boxin Wei Jin Xu Liqun Gao Hui Feng Jiajun Wu Cheng Sun Zhenyao Wang Wei Ke | 2022 | Journal of Materials Science & Technology2022，，12： | 2 |
| 19 | Research on Government Compensation for Toll Road Public-Private Partnerships (PPP) Projects显示文摘With the continuous implementation of the PPP projects,due to the imperfection of relevant policies,the blindness of government subsidy is constantly emerging.Thus,it is important to put forward a practical approach for valuing the subsidies and risk.In this paper,the revenue subsidies model and traffic subsidies model are established.Then combined with practical cases,Monte Carlo simulation is used to get the value and probability of government subsidies under different compensation ways.On this basis,the influence of initial traffic volume and traffic growth rate on government subsidies and net present value of investors is analyzed.The research findings can provide theoretical guidance for the government to choose a reasonable way of subsidies,balance the risk of compensation,and formulate subsidies policies. | Zhenyao Wu Bin Shuai | 2020 | Frontiers Research of Architecture and Engineering2020，3，2： | 2 |
| 20 | Seawater hydraulics:From the sea surface to depths of 11000 meters显示文摘Using seawater as the working medium,the seawater hydraulic system has significant advantages in the deep sea,such as eliminating contamination caused by oil leaks,no oil tanks,and the ability to automatically adapt to sea depths.As working depths increase,seawater hydraulic technology faces enormous challenges.First,the physical and chemical properties of seawater,such as density,temperature,and composition,change significantly.Second,hydraulic components are subject to an environmental pressure of up to 110 MPa,which causes serious deformation or even seizure of moving pairs and significantly affects the efficiency of hydraulic components.The friction and wear characteristics between the moving pairs also significantly change with the change in sea depth,therefore developing the matching material according to depth is necessary.Finally,the cavitation characteristic of a valve port is obviously different from that on land,so special material and structure should be used to improve the service life of the valve port.These factors severely restrict the improvement of the working depths and characteristics of seawater hydraulic components.This article focuses on the abovementioned problems and provides solutions for deep-sea seawater hydraulic technology. | LIU YinShui CHENG Qian WANG ZhenYao PANG Hao DENG YiPan ZHOU XinPing LUO XiaoHui CUI Yan WU DeFa | 2022 | Science China(Technological Sciences)2022，65，9： | 2 |
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