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| 1 | Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research. | LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER | 2008 | Chinese Science Bulletin2008，53，7： | 8 |
| 2 | Hydrogen generation from decomposition of hydrous hydrazine over Ni-Ir/CeO_2 catalyst显示文摘The synthesis of highly active and selective catalysts is the central issue in the development of hydrous hydrazine(N_2H_4·H_2O) as a viable hydrogen carrier. Herein, we report the synthesis of bimetallic Ni-Ir nanocatalyts supported on CeO_2 using a one-pot coprecipitation method. A combination of XRD, HRTEM and XPS analyses indicate that the Ni-Ir/CeO_2 catalyst is composed of tiny Ni-Ir alloy nanoparticles with an average size of around 4 nm and crystalline CeO_2 matrix. The Ni-Ir/CeO_2 catalyst exhibits high catalytic activity and excellent selectivity towards hydrogen generation from N_2H_4·H_2O at mild temperatures. Furthermore, in contrast to previously reported Ni-Pt catalysts, the Ni-Ir/CeO_2 catalyst shows an alleviated requirement on alkali promoter to achieve its optimal catalytic performance. | Hongbin Dai Yujie Zhong Ping Wang | 2017 | Progress in Natural Science:Materials International2017，27，1： | 2 |
| 3 | Effect of river plume on phytoplankton community structure in Zhujiang River estuary显示文摘To examine the phytoplankton assemblages and the effect of diluted waters on them,a research cruise was conducted from July 19 to August 7,2015 in the Zhujiang(Pearl)River estuary in the northern South China Sea(21°N–23.5°N,111°E–117°E).Samples were collected from 65 stations including one for time-series sampling.A total of 212 phytoplankton taxa were identified from 61 genera belonging to 4 phyla.Among them,122 species identified from 42 genera of Bacillariophyta and 83 species from 15 genera of Pyrrophyta.Chain-forming diatoms dominated the phytoplankton community where Pseudonitzschia delicatissima,Guinardia striata,Thalassionema nitzschioides,and P.pungens comprised about 52%of the total abundance.However,higher cell abundances concentrated on both sides of the estuary,because of low salinity and high nutrients brought by diluted water.In addition,Canonical Correspondence Analysis revealed that salinity and dissolved inorganic nitrogen shaped the species composition in the study area.Furthermore,the Jaccard similarity index showed prevailing high similarity in the distribution of species in low-salinity diluted waters,and the Bray-Curtis similarity depicted distinguished grouping for phytoplankton assemblages along the salinity gradient.However,phytoplankton diel vertical cycles showed maximum abundance occurred at 2:00 am,which was mainly contributed by benthic phytoplankton species Pseudo-nitzschia spp.and T.nitzschioides. | Qi ZHONG Bing XUE Md Abu NOMAN Yuqiu WEI Haijiao LIU Hongbin LIU Liping ZHENG Hongmei JING Jun SUN | 2021 | Journal of Oceanology and Limnology2021，39，2： | 2 |
| 4 | Optimal time for subarachnoid transplantation of neural progenitor cells in the treatment of contusive spinal cord injury显示文摘This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour (acute stage), 7 days (subacute stage) and 28 days (chronic stage) after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. O4- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for O4 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages. | Yan Liu Ying Zhou Chunli Zhang Feng Zhang Shuxun Hou Hongbin Zhong Hongyun Huang | 2013 | Neural Regeneration Research2013，8，5： | 2 |
| 5 | 显示文摘 | Zhong Jiuping Liang Hongbin Han Bing | 2008 | Opt Expr- ess2008，16，10： | 1 |
| 6 | Chemical signals based on multi-channel wavelet denoising method 显示文摘 | ZHONG Hongbin ZHOU Changli LI Guanbin | 2006 | Computer and Applied Chemistry2006，23，10： | 1 |
| 7 | Reconstructed bladder innervation below the level of spinal cord injury:the knee-tendon to bladder artificial reflex arc显示文摘 | Zheng Xianyou Hou Chunlin Zhong Hongbin | | 0，，01： | 1 |
| 8 | Product platform commonization: platform construction and platform elements capture显示文摘 | Hongbin Qin Yifang Zhong Renbin Xiao Weiguo Zhang | 2005 | The International Journal of Advanced Manufacturing Technology (-)2005，，11： | 1 |
| 9 | A wide band- width circularly polarized microstrip antenna array using se- quentially rotated feeding technique 显示文摘 | Yu Tongbin Li Hongbin Zhong Xinjian | 2013 | IEEE Transactions on Antennas Propagram2013，，2： | 1 |
| 10 | Product platform commonization: platform construction and platform elements capture显示文摘 | Hongbin Qin Yifang Zhong Renbin Xiao Weiguo Zhang | 2005 | The International Journal of Advanced Manufacturing Technology (-)2005，，11： | 1 |
| 11 | Simultaneous Enhancement of Open‐Circuit Voltage, Short‐Circuit Current Density, and Fill Factor in Polymer Solar Cells显示文摘 | Zhicai He Chengmei Zhong Xun Huang Wai‐Yeung Wong Hongbin Wu Liwei Chen Shijian Su Yong Cao | 2011 | Adv Mater2011，，40： | 1 |
| 12 | Improving photovoltaic parameters of all-polymer solar cells through integrating two polymeric donors显示文摘The incorporation of an additional component into the bulk-heterojunction light-harvesting layer of polymer solar cells has been considered as an effective strategy to enhance photovoltaic performance.Here we demonstrated that the photovoltaic parameters of all-polymer solar cells could be enhanced upon replacing a certain ratio of electron-donating polymer PTz BI-o F with a widely used wide-bandgap polymer donor PM6.The photoluminescent characterizations confirmed the F?rster resonance energy transfer from incorporated PM6 to PTz BI-o F.Moreover,the combination of Fourier-transform photocurrent spectroscopy and electroluminescence external quantum efficiencies measurements demonstrated reduced non-radiative recombination energy loss upon the incorporation of PM6,resulting in a slightly enhanced open-circuit voltage of 0.88 V of the ternary cell regarding the binary PTz BI-o F:PFA1 device.The optimized ternary blend devices comprising of PTz BI-o F:PM6:PFA1 presented an impressively high power conversion efficiency of 16.3%,and the efficiency remains 15%on a device with an enlarged effective area of 1 cm^(2),demonstrating the great potential of these all-PSCs for potential applications. | Kang An Feng Peng Wenkai Zhong Wanyuan Deng Difei Zhang Lei Ying Hongbin Wu Fei Huang Yong Cao | 2021 | Science China Chemistry2021，64，11： | 1 |
| 13 | Investigation of lateral spreading current in the 4H-SiC Schottky barrier diode chip显示文摘Lateral current spreading in the 4H-SiC Schottky barrier diode(SBD)chip is investigated.The 4H-SiC SBD chips with the same vertical parameters are simulated and fabricated.The results indicate that there is a fixed spreading resistance at on-state in current spreading region for a specific chip.The linear specific spreading resistance at the on-state is calculated to be 8.6Ω/cm in the fabricated chips.The proportion of the lateral spreading current in total forward current(Psp)is related to anode voltage and the chip area.Psp is increased with the increase in the anode voltage during initial on-state and then tends to a stable value.The stable values of Psp of the two fabricated chips are 32%and 54%.Combined with theoretical analysis,the proportion of the terminal region and scribing trench in a whole chip(Ksp)is also calculated and compared with Psp.The Ksp values of the two fabricated chips are calculated to be 31.94%and 57.75%.The values of Ksp and Psp are close with each other in a specific chip.The calculated Ksp can be used to predict that when the chip area of SiC SBD becomes larger than 0.5 cm2,the value of Psp would be lower than 10%. | Xi Wang Yiwen Zhong Hongbin Pu Jichao Hu Xianfeng Feng Guowen Yang | 2021 | Journal of Semiconductors2021，42，11： | 1 |
| 14 | Near field acoustic solution of a rotating point source in frequency domain显示文摘On the basis of the frequency domain acoustic solation of sources in arbitrary motion, in this paper an approximate acoustic solution of a rotating point source in near field is given, which is the superposition of the well-Known far field solution and three near field modification items on the condition that the rotating radius is acoustically compact compared with the field sound wavelength. Accordingly the near field Green function in free space is obtained. The characteristic directionality of the sound field induced by source rotating is discussed, and the influences of source position, source frequency and rotating frequency are studied in detail. | JU Hongbin SHEN Mengyu ZHONG Fangyuan(Tsinghua Uaiversity Beijng 100084) (Sbanghai Jiaotong University Shanghai 200031) | 1996 | Chinese Journal of Acoustics1996，15，3： | 0 |
| 15 | Hybrid Network Model Based on Data Enhancement for Short-term Power Prediction of New PV Plants显示文摘This study proposes a hybrid network model based on data enhancement to address the problem of low accuracy in photovoltaic(PV)power prediction that arises due to insuffi cient data samples for new PV plants.First,a time-series gener ative adversarial network(TimeGAN)is used to learn the distri bution law of the original PV data samples and the temporal correlations between their features,and these are then used to generate new samples to enhance the training set.Subsequently,a hybrid network model that fuses bi-directional long-short term memory(BiLSTM)network with attention mechanism(AM)in the framework of deep&cross network(DCN)is con structed to effectively extract deep information from the origi nal features while enhancing the impact of important informa tion on the prediction results.Finally,the hyperparameters in the hybrid network model are optimized using the whale optimi zation algorithm(WOA),which prevents the network model from falling into a local optimum and gives the best prediction results.The simulation results show that after data enhance ment by TimeGAN,the hybrid prediction model proposed in this paper can effectively improve the accuracy of short-term PV power prediction and has wide applicability. | Shangpeng Zhong Xiaoming Wang Bin Xu Hongbin Wu Ming Ding | 2024 | Journal of Modern Power Systems and Clean Energy2024，12，1： | 0 |
| 16 | Biliary candidiasis mimicking IgG4-related disease in a patient with normal immunity显示文摘Purpose:To report a rare case of biliary candidiasis in a host with normal immunity.Methods:Laboratory tests indicated an elevated G level of 1000 pg/ml.Microscopic examination of pathological sections demonstrated multiple,narrow base,budding yeast cells following Grocott's methenamine silver staining and periodic acid-Schiff staining.Subsequent sequence analysis supported the diagnosis of biliary candidiasis.Results:A 31-year-old previously healthy man developed obstructive jaundice under no obvious inducement.He experienced cholecystectomy and biliary drainage.Surgical specimens revealed granulomatous chronic inflammation in the gallbladder,liver,bile ducts and mesenteric lymph nodes.He was misdiagnosed with tuberculosis and received antituberculosis treatment.However,his condition did not improve.He was finally diagnosed with biliary candidiasis and administered the antifungal treatment with fluconazole.His symptoms improved after such treatment.Conclusions:We reported a case of biliary candidiasis that mimicked IgG4-RD in a host with normal immunity.Our findings highlighted the need for pathological diagnosis in patients with symptoms resembling IgG4-RD. | Wenjie Bian Yuchao Zhong Xia Zhang Hongbin Chen Ruie Feng Xinyu Zhang Danhua Shen Yanying Liu | 2022 | Rheumatology & Autoimmunity2022，2，3： | 0 |
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