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| 1 | Molecular Epidemiological Analysis of Echovirus 19 Isolated From an Outbreak Associated With Hand, Foot, and Mouth Disease (HFMD) in Shandong Province of China显示文摘Objective To elucidate the genetic characterization and molecular epidemiological features of Echovirus 19(E19)isolates collected from an outbreak associated with hand,foot and mouth disease(HFMD)in Tai'an city of Shandong Province of China from July to September,2003.Methods Thirty seven Echovirus 19 isolates were isolated from stool specimens and throat swabs collected during the outbreak,then major capsid(VP1)genomic sequence was determined,and phylogenetic tree was done based on the VP1 sequences among these 37 and other E19 viruses deposited in the Genbank.Also a representative strain named CHN-SD03-TN12 was selected for sequencing of 5'-untranslated regions(5'-UTR).Results The identity rate was about 98.9%-100% among all these 37 E19 viruses.The genetic relationships between these 37 E19 isolates and other strains reported were also depicted.The identity rate was about 78.4%-78.9% compared with E19 reference strain Burke.The substitutions in the sequence of 5'-UTR resulted in changes in the conjectural properties of 5'-UTR of E19 viruses.Conclusion The genetic features of E19 viruses isolated during the outbreak in Shandong Province in 2003 may be associated with a genetic and antigenic drift that changes the virulence of the Shandong isolates,but the molecular changes in Shandong E19 viruses contributing to their phenotype remain to be further illuminated.However,the sequences described in this paper substantiate the changes taken place in capsid VP1 and 5'UTR regions.These substitutions may contribute to their tropism and virulence,and play a significant role in pathogenesis and clinical manifestations of the disease. | ZHEN ZHU WEN-BO XU AI-QIANG XU HAI-YAN WANG YONG ZHANG LI-ZHI SONG Hui-LiYANG YANLI FENGJI | 2007 | Biomedical and Environmental Sciences2007，20，4： | 22 |
| 2 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 3 | Genetic Variation of the VP1 Gene of the Virulent Duck Hepatitis A Virus Type 1(DHAV-1) Isolates in Shandong Province of China显示文摘To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change. | Jiming Gao Junhao Chen Xingkui Si Zhijing Xie Yanli Zhu Xingxiao Zhang Shujing Wang Shijin Jiang | 2012 | Virologica Sinica2012，27，4： | 10 |
| 4 | Clinical applications of MARSALA for preimplantation genetic diagnosis of spinal muscular atrophy显示文摘Conventional PCR methods combined with linkage analysis based on short tandem repeats(STRs) or Karyomapping with single nucleotide polymorphism(SNP) arrays, have been applied to preimplantation genetic diagnosis(PGD) for spinal muscular atrophy(SMA), an autosome recessive disorder. However, it has limitations in SMA diagnosis by Karyomapping, and these methods are unable to distinguish wildtype embryos with carriers effectively. Mutated allele revealed by sequencing with aneuploidy and linkage analyses(MARSALA) is a new method allowing embryo selection by a one-step next-generation sequencing(NGS) procedure, which has been applied in PGD for both autosome dominant and X-linked diseases in our group previously. In this study, we carried out PGD based on MARSALA for two carrier families with SMA affected children. As a result, one of the couples has given birth to a healthy baby free of mutations in SMA-causing gene. It is the first time that MARSALA was applied to PGD for SMA, and we can distinguish the embryos with heterozygous deletion(carriers) from the wild-type(normal) ones accurately through this NGS-based method. In addition, direct mutation detection allows us to identify the affected embryos(homozygous deletion), which can be regarded as probands for linkage analysis, in case that the affected family member is absent. In the future, the NGS-based MARSALA method is expected to be used in PGD for all monogenetic disorders with known pathogenic gene mutation. | Yixin Ren Xu Zhi Xiaohui Zhu Jin Huang Ying Lian Rong Li Hongyan Jin Yan Zhang Wenxin Zhang Yanli Nie Yuan Wei Zhaohui Liu Donghong Song Ping Liu Jie Qiao Liying Yan | 2016 | Journal of Genetics and Genomics2016，43，9： | 9 |
| 5 | Status and development of high-power laser facilities at the NLHPLP显示文摘In this paper, we review the status of the multifunctional experimental platform at the National Laboratory of High Power Laser and Physics(NLHPLP). The platform, including the SG-II laser facility, SG-II 9th beam, SG-II upgrade(SG-II UP) facility, and SG-II 5 PW facility, is operational and available for interested scientists studying inertial confinement fusion(ICF) and a broad range of high-energy-density physics. These facilities can provide important experimental capabilities by combining different pulse widths of nanosecond, picosecond, and femtosecond scales. In addition, the SG-II UP facility, consisting of a single petawatt system and an eight-beam nanosecond system, is introduced including several laser technologies that have been developed to ensure the performance of the facility. Recent developments of the SG-II 5 PW facility are also presented. | Jianqiang Zhu Jian Zhu Xuechun Li Baoqiang Zhu Weixin Ma Xingqiang Lu Wei Fan Zhigang Liu Shenlei Zhou Guang Xu Guowen Zhang Xinglong Xie Lin Yang Jiangfeng Wang Xiaoping Ouyang Li Wang Dawei Li Pengqian Yang Quantang Fan Mingying Sun Chong Liu Dean Liu Yanli Zhang Hua Tao Meizhi Sun Ping Zhu Bingyan Wang Zhaoyang Jiao Lei Ren Daizhong Liu Xiang Jiao Hongbiao Huang Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，4： | 8 |
| 6 | Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses显示文摘Human skeletal stem cells(SSCs)have been discovered in fetal and adult long bones.However,the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive.Here we map the transcriptional landscape of human limb buds and embryonic long bones at single-cell resolution to address this fundamental question.We found remarkable heterogeneity within human limb bud mesenchyme and epithelium,and aligned them along the proximal-distal and anterior-posterior axes using known marker genes.Osteo-chondrogenic progenitors first appeared in the core limb bud mesenchyme,which give rise to multiple populations of stem/progenitor cells in embryonic long bones undergoing endochondral ossification.Importantly,a perichondrial embryonic skeletal stem/progenitor cell(eSSPC)subset was identified,which could self-renew and generate the osteochondral lineage cells,but not adipocytes or hematopoietic stroma.eSSPCs are marked by the adhesion molecule CADM1 and highly enriched with FOXP1/2 transcriptional network.Interestingly,neural crest-derived cells with similar phenotypic markers and transcriptional networks were also found in the sagittal suture of human embryonic calvaria.Taken together,this study revealed the cellular heterogeneity and lineage hierarchy during human embryonic skeletogenesis,and identified distinct skeletal stem/progenitor cells that orchestrate endochondral and intramembranous ossification. | Jian He Jing Yan Jianfang Wang Liangyu Zhao Qian Xin Yang Zeng Yuxi Sun Han Zhang Zhijie Bai Zongcheng Li Yanli Ni Yandong Gong Yunqiao Li Han He Zhilei Bian Yu Lan Chunyu Ma Lihong Bian Heng Zhu Bing Liu Rui Yue | 2021 | Cell Research2021，31，7： | 5 |
| 7 | The Bethesda System for Reporting Thyroid Cytopathology(TBSRTC):A report of 2,781 cases in a Chinese population显示文摘Objective:To evaluate the diagnostic utility of The Bethesda System for Reporting Thyroid Cytology(TBSRTC)at Peking University Cancer Hospital,the incidence of noninvasive follicular thyroid neoplasms with papillary-like nuclear features(NIFTP),and the impact of reclassification on cytopathological outcomes.Methods:We performed a retrospective study of thyroid fine-needle aspiration(FNA)cases between April 2014 and March 2019.The FNA results were classified according to TBSRTC.Post-surgery histological findings were followed up.Results:A total of 2,781 thyroid FNAs were performed.The incidences of the 6 diagnostic categories(DCs I-VI)were 14.8%,17.1%,15.8%,2.3%,11.6%and 38.5%,respectively.A total of 1,122 patients(40.3%)had corresponding histological results.NIFTP accounted for 0.4%of papillary thyroid carcinoma(PTC)cases,and there was no significant difference in the risk of malignancy(ROM)for each TBSRTC DC between'NIFTP=carcinoma(Ca)'and'NIFTP≠Ca'.When'NIFTP=Ca',the sensitivity,specificity,positive predictive value(PPV),negative predictive value(NPV),and accuracy of TBSRTC were 98.0%,84.0%,99.4%,58.3%,and97.5%,respectively.When'NIFTP≠Ca',the sensitivity,specificity,PPV,NPV and accuracy of the TBSRTC were 98.1%,81.5%,99.3%,61.1%,and 97.5%,respectively.Conclusions:TBSRTC is effective in the preoperative diagnosis of thyroid nodules in Peking University Cancer Hospital.The impact of the reclassification of NIFTP on cytopathological outcomes is limited because of its low incidence,and the revised ROMs are not suitable for Asian patients. | Yanli Zhu Yuntao Song Guohui Xu Zhihui Fan Wenhao Ren | 2020 | Chinese Journal of Cancer Research2020，32，2： | 4 |
| 8 | Genetic testing and PGD for unexplained recurrent fetal malformations with MAGEL2 gene mutation显示文摘Birth defects are caused by multiple factors,such as chromosome abnormality,environmental factors,and maternal factors.In this study,we focused on exploring the genetic causes of a non-consanguineous couple who suffered from four times of unsuccessful pregnancy due to unexplained recurrent fetal malformations with similar symptoms and normal chromosome copy number variations.Using trio-whole exome sequencing(trio-WES) for this couple and one of the affected fetuses,we found a mutation,c.1996 delC on the maternal imprinted gene MAGEL2 that was carried by the affected fetus and husband,leading to Schaaf-Yang syndrome.To screen this mutation,we further performed preimplantation genetic diagnosis(PGD) strategy followed by a gene pedigree validation and pathogenicity analysis.After the transfer of a PGD-screened embryo,a normal newborn without previous abnormal symptoms was born(February 15,2019).We present the first data that identified a pathogenic gene(MAGEL2 c.1996 delC) in a fetus with Schaaf-Yang syndrome in the EAS(East Asian) database and overcame this genetic defect by using processed PGD for this couple based on the WES results. | Wei Guo Yanli Nie Zhiqiang Yan Xiaohui Zhu Yuqian Wang Shuo Guan Ying Kuo Wenxin Zhang Xu Zhi Yuan Wei Liying Yan Jie Qiao | 2019 | Science China(Life Sciences)2019，62，7： | 4 |
| 9 | Identifying normal embryos from reciprocal translocation carriers by whole chromosome haplotyping显示文摘Reciprocal translocation is a chromosomal structural abnormality that arises when two non-homologous chromosomes rearrange and attach with each other, an incidence that occurs in about 1/500to 1/625 newborns (Mackie and Scriven, 2002). This event typically does not lead to any significant loss of genetic material, thus reciprocal translocation carriers do not exhibit any severe | Zhiqiang Yan Yuqian Wang Yanli Nie Xu Zhi Xiaohui Zhu Meng Qin Shuo Guan Yixin Ren Ying Kuo Di Chang Wei Chen Peng Yuan Liying Yan Jie Qiao | 2018 | Journal of Genetics and Genomics2018，45，9： | 4 |
| 10 | Anti-tumor effect of the extract from Qingyihuaji formula on pancreatic cancer by down-regulating Notch-4 and Jagged-1显示文摘OBJECTIVE: To investigate, in terms of Notch signaling pathway, the effect on pancreatic cancer of the extract of an anti-tumor prescription — Qingyihuaji formula(QYHJ) — from Traditional Chinese Medicine(TCM).METHODS: Nude mice were implanted subcutaneously with human pancreatic cancer cell line SW1990 and then randomly divided into four groups: Control, QYHJ extract, Gemcitabine, and Combination of QYHJ extract and gemcitabine.Treatments were given for 21 days and tumor growth was evaluated simultaneously. Then, expression of Notch receptors(Notch-1, Notch-2,Notch-3, and Notch-4) and their Jagged ligands(Jagged-1 and Jagged-2) in dissected tumor tissuewere detected by real-time quantitative reverse transcription-polymerase chain reaction and Western blot. Finally, immunohistochemistry was performed to detect CD133, a marker of pancreatic cancer stem cells(CSCs), to evaluate the impact of QYHJ extract on pancreatic CSCs.RESULTS: QYHJ extract treatment effectively inhibited the tumor growth in nude mice. The expression of both Notch-4 and Jagged-1 were decreased significantly in QYHJ treatment groups(P < 0.05), while gemcitabine alone had no significant effect in down-regulating Jagged-1(P > 0.05).No significant difference was observed in the expression of Notch-1, Notch-2, Notch-3, and Jagged-2 between three treatment groups and control group(P > 0.05). Moreover, immunohistochemical analysis showed that the number of CD133 positive cells was significantly reduced by QYHJ treatment(P < 0.05), and the combined treatment was more effective than gemcitabine alone(P < 0.05).CONCLUSION: The role of the extract in pancreatic cancer treatment was associated with down-regulation of Notch-4 and Jagged-1 in Notch signaling pathway. The extract could enhance the antitumor activity of gemcitabine and was more effective than gemcitabine in regulating Notch signaling pathway to some extent. | Xu Yanli Zhu Feiye Xu Shan Liu Luming | 2015 | Journal of Traditional Chinese Medicine2015，35，1： | 4 |
| 11 | Spatio-temporal evolution of the optical field on a hohlraum wall at the rising edge of a flat-topped pulse显示文摘Considering the time delay in different hohlraum wall positions caused by oblique incidence,the spatio-temporal optical field distribution characteristics of a hohlraum wall,especially during the rising edge of a flat-topped pulse,is simulated by a fast Fourier transform method together with chromatography.Results demonstrate that beam propagation along the hohlraum wall is a push-broom process with complex dynamic spatial–temporal evolution.In the first few picoseconds,the optical intensity of the front position increases rapidly,while that of the rear position is relatively weak.The ratio R of the optical intensity during the rising edge is smaller than that of the steady state.R gradually increases and finally tends to the value of the steady state with time.Calculation also shows that,with shorter total width of the rising edge,R of the optical field decreases and the difference compared to the steady state becomes larger.The evolution is more severe with smaller angle of inclination. | Zhaoyang Jiao Yanli Zhang Junyong Zhang Jianqiang Zhu | 2013 | High Power Laser Science and Engineering2013，1，2： | 3 |
| 12 | Antifungal properties and mechanisms of three volatile aldehydes(octanal,nonanal and decanal)on Aspergillus flavus显示文摘The harmof Aspergillus flavus(A.flavus)and aflatoxin is a severe food safety problemworldwide,which causes huge economic losses every year.Therefore,it is urgent to control the growth of A.flavus and the biosynthesis of aflatoxin.Plant-derived natural compounds are superior to synthetic fungicide in inhibiting the growth of A.flavus benefiting from their high safety to the environment,humans and stock,and low cost.This study aimed to evaluate the antifungal effects and potential antifungal mechanisms of three plant-derived compounds(octanal,nonanal and decanal)against A.flavus.We determined the minimum inhibitory concentrations(MICs)and action mechanism of the three volatile aldehydes on A.flavus and also performed calcofluor white(CW)staining for visualizing the distribution of septa.Cell respiration metabolism and the pathogenicity on maize kernels were also carried out to evaluate the efficacy of the three volatile aldehydes on the growth of A.flavus.The results showed that the three volatile aldehydes could inhibit the germination of spores and mycelial growth of A.flavus,the MICs on spores and mycelia were:octanal(1.0 and 0.5μL/mL),nonanal(0.5 and 2.0μL/mL),and decanal(1 and 5μL/mL).The three volatile aldehydes could strongly damage the integrity of both the cell wall and the cell membrane of A.flavus.Meanwhile,they could decrease the content of total lipid and inhibit respiration metabolism of A.flavus cell.Results of in vitro antifungal test showed that all the three volatile aldehydes could effectively prevent the growth of A.flavus on maize kernels.The study revealed that octanal,nonanal and decanal could effectively inhibit the growth of A.flavus both in culture medium and on maize kernels to different extent.The results confirmed that the plant-derived compounds could be developed into promising antifungal agents applied in the preservation of grains.This study provides a theoretical basis for the research and application of potential antifungal agents. | Qian Li Xiaoman Zhu Yanli Xie Jingmeng Liang | 2021 | Grain & Oil Science and Technology2021，4，3： | 2 |
| 13 | The open-close mechanism of M2 channel protein in influenza A virus:A computational study on the hydrogen bonds and cation-π interactions among His37 and Trp41显示文摘The M2 protein from influenza A virus is a tetrameric ion channel. It was reported that the permeation of the ion channel is correlated with the hydrogen bond network among His37 residues and the cation-π interactions between His37 and Trp41. In the present study,the hydrogen bonding network of 4-methyl-imidazoles was built to mimic the hydrogen bonds between His37 residues,and the cation-π interactions between 4-methyl-imidazolium and indole systems were selected to represent the interac-tions between His37 and Trp41. Then,quantum chemistry calculations at the MP2/6-311G level were carried out to explore the properties of the hydrogen bonds and the cation-π interactions. The calcula-tion results indicate that the binding strength of the N-H···N hydrogen bond between imidazole rings is up to -6.22 kcal·mol-1,and the binding strength of the strongest cation-π interaction is up to -18.8 kcal·mol-1(T-shaped interaction) or -12.3 kcal·mol-1(parallel stacking interaction). Thus,the calcu-lated binding energies indicate that it is possible to control the permeation of the M2 ion channel through the hydrogen bond network and the cation-π interactions by altering the pH values. | CHENG JiaGao ZHU WeiLiang WANG YanLi YAN XiuHua LI Zhong TANG Yun JIANG HuaLiang | 2008 | Science China Chemistry2008，51，8： | 2 |
| 14 | A novel pathogenic mutation in FBN2 associated with congenital contractural arachnodactyly for preimplantation genetic diagnosis显示文摘Congenital contractural arachnodactyly(CCA,OMIM:121050),also known as Beals syndrome,belongs to a group of rare autosomal dominant(AD)diseases of connective tissue(Maslen et al.,1997).People with CCA share many distinguishing features,such as arachnodactyly,camp tod actyly,multiple joint contractures(especially finger,elbow,and knee joints),crumpled ears,scoliosis,pectus deformities,and muscular hypoplasia(Jurko et al.,2013).It exhibits no specific geographic or ethnic predilection(Frederic et al.,2009). | Jiaxin Li Yuqian Wang Xiaohui Zhu Yanli Nie Ying Kuo Shuo Guan Jin Huang Ying Lian Yangyu Zhao Rong Li Yuan Wei Jie Qiao Liying Yan | 2020 | Journal of Genetics and Genomics2020，47，5： | 2 |
| 15 | Evolution of low-frequency noise passing through a spatial filter in a high power laser system显示文摘The theoretical model of spatial noise passing through a spatial filter is established in high power laser system under the small signal approximation. The transmission characteristic for a noise signal passing through spatial filters with different magnifications is analyzed by numerical simulation, according to the actual structure of the high power laser system. The results show that the spatial modulation period of low-frequency noise getting through the pinhole will be proportional to the magnification of the spatial filter. When the magnification is less than 1, the safe low-frequency noise will be extruded into the high-frequency region, which is the fast increasing part, and finally develops into the most dangerous part which can damage the optical devices. The conclusion of this research improves the relay imaging theory of a spatial filter and provides an important theoretical basis for a general design of high power laser systems. | SUN PingPing LIU DeAn ZHANG YanLi LI XiaoYan ZHANG Yan ZHU JianQiang | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，3： | 2 |
| 16 | Impact of long-term chemical fertilizer and organic amendment to Fusarium root rot of soybean显示文摘Soil suppressiveness to Fusarium root rot of soybean had been observed in a black soil field after a long-term fertilization with nitrogen(N)and phosphorus(P)fertilizer combined with pig manure as organic amendment(NPM),rather than that with only nitrogen and phosphorus fertilizer(NP)or no fertilizer(NF).To determine the microbial role on this suppressiveness,fungal and bacterial community characteristics in NPM,NP and NF treatments were investigated by q PCR and DGGE.Compared with the similar bacterial community characteristics among 3 treatments,fungal community,especially Fusarium population size and community composition in NPM treatment were different with those of NP and NF groups.Based on the isolation and pathogenicity test,pathogenic F.oxysporum,F.graminearum,F.verticillioide and F.lateritium absolutely dominated Fusarium community in NF and NP groups.Nonpathogenic F.avenaceum,F.equiseti,F.culmorum,F.redolens,F.solani and F.tricinctum dominated Fusarium community in NPM group.Isolation rate of pathogenic Fusarium in NPM reduced from 100%to 38%in NF.These results suggested that the dominance of soil non-pathogenic Fusarium population induced by organic amendment might play an important role on suppressing Fusarium root rot in the tested field. | Kaili Wang Xinyu Hu Sai Yang Kaiyan Xing Xin Zhang Lin Zhu Xiaozeng Han Yanli Xu Wei Wei | 2020 | Oil Crop Science2020，5，1： | 2 |
| 17 | Advanced glycation end-product N ? -carboxymethyl-Lysine accelerates progression of atherosclerotic calcification in diabetes显示文摘 | Zhongqun Wang Yicheng Jiang Naifeng Liu Liqun Ren Yinghong Zhu Yanli An Dengyu Chen | 2012 | Atherosclerosis2012，，2： | 2 |
| 18 | Activation of the bitter taste sensor TRPM5 prevents high saltinduced cardiovascular dysfunction显示文摘High salt intake is a known risk factor of cardiovascular diseases. Our recent study demonstrated that long-term high salt intake impairs transient receptor potential channel M5(TRPM5)-mediated aversion to high salt concentrations, consequently promoting high salt intake and hypertension;however, it remains unknown whether TRPM5 activation ameliorates cardiovascular dysfunction. Herein we found that bitter melon extract(BME) and cucurbitacin E(CuE), a major compound in BME, lowered high salt-induced hypertension. Long-term BME intake significantly enhanced the aversion to high salt concentrations by upregulating TRPM5 expression and function, eventually decreasing excessive salt consumption in mice. Moreover, dietary BME ameliorated high salt-induced cardiovascular dysfunction and angiotensin II-induced hypertension in vivo. The mechanistic evidence demonstrated that dietary BME inhibited high salt-induced RhoA/Rho kinase pathway overactivation, leading to reduced phosphorylation levels of myosin light chain kinase and myosin phosphatase targeting subunit 1. Furthermore, CuE inhibited vasoconstriction by attenuating L-type Ca^(2+) channel-induced Ca^(2+) influx in vascular smooth muscle cells. To summarize, our findings indicate that dietary BME has a beneficial role in antagonizing excessive salt consumption and thus appears promising for the prevention of high salt-induced cardiovascular dysfunction. | Hao Wu Yuanting Cui Chengkang He Peng Gao Qiang Li Hexuan Zhang Yanli Jiang Yingru Hu Xiao Wei Zongshi Lu Tianyi Ma Daoyan Liu Zhiming Zhu | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
| 19 | Fast screening compositions of PM2.5 by ATR-FTIR: Comparison with results from IC and OC/EC analyzers显示文摘Chemical speciation of fine particles or PM_(2.5) collected on filters is still a costly and timeconsuming task. In this study, filter-based PM_(2.5) samples were collected during November–December 2013 at four sites in Guangzhou, and the major components were fast screened(~7 min per filter sample) by Attenuated Total Reflectance(ATR)-Fourier Transform Infrared Spectroscopic(FTIR) in comparison with that measured by Organic carbon/Element carbon(OC/EC) analyzer and Ion Chromatography(IC). The concentrations of nitrate, ammonium, sulfate,primary organic carbon(POC) and secondary organic carbon(SOC) measured by OC/EC and IC analyzers were better correlated with their infrared absorption peak heights at 1320 cm^(-1) for nitrate, 1435, 3045 and 3215 cm^(-1) for ammonium, 615 cm^(-1) for sulfate, 690, 760 and 890 cm^(-1) for POC and 1640 and 1660 cm^(-1) for SOC respectively, during polluted days(PM_(2.5)> 75 μg/m^3) than during clean days(PM_(2.5)≤ 75 μg/m^3). With the evolution of a haze episode during our field campaign, the concentrations of the major PM_(2.5) components displayed consistent variations with their infrared absorption peak heights, suggesting ATR-FTIR could be a fast and useful technique to characterize filter-based PM_(2.5) compositions particularly during pollution events although cautions should be taken when PM_(2.5) levels are low. Notably, elevated PM_(2.5) mass concentrations occurred with enhanced ratios of [NO_(-3)]/[SO_4^(2-)] and [NH^(+4)]/[SO_4^(2-)], implying that nitrogenous components play vital roles in the PM_(2.5) pollution events in the study region. | Xu Yu Wei Song Qingqing Yu Sheng Li Ming Zhu Yanli Zhang Wei Deng Weiqiang Yang Zhonghui Huang Xinhui Bi Xinming Wang | 2018 | Journal of Environmental Sciences2018，30，9： | 2 |
| 20 | Analysis on the environmental effect of renewable energy consumption by rural residents in daily life in China-from the perspectives of carbon emissions显示文摘 | Chen Yan Zhu Yanli | 2011 | Energy Procedia2011，，： | 2 |
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